PHLEBOTOMY

Hematology 1 (Laboratory)
BS Medical Laboratory Science | Abella, Pelis Florbien A. | SEM 1 2023-2024

❖ 21: Purple
PHLEBOTOMY ❖ 22: Blue
❖ 23: Orange
❖ Act of drawing or removing blood from ❖ 25: Red
the circulatory system.
❖ Purpose:
1. Diagnostic purposes ORDER OF DRAW
2. Monitor blood components ❖ Yellow, Blue, Red, Green, Lavender,
3. Treat blood-related disorders Gray
4. Remove excess of blood due to
↑ of iron or erythrocytes LABEL OF TUBES
5. Collect blood for later uses
6. Administer therapeutic ❖ Px Name, age, sex, time, date,
Phlebotomist’s initial
treatment

PERIPHERAL BLOOD SMEAR

BLOOD COLLECTION METHOD
❖ Peripheral blood film
❖ Syringe - A thin layer of blood smear on a
❖ ETS
glass slide and then stain.
❖ Skin puncture
- Used for:
1. Morphological
PUNCTURE SITE evaluation of blood cells
❖ Median vein 2. WBC differential count
- Antecubital fossa; elbow pit 3. Platelet estimation
❖ Cephalic 4. Verifying automation
❖ Basilic results
5. At least 2 smears are
prepared
EVACUATED TUBE METHOD
❖ Method that allows multiple tube
collection with one needle puncture. 3 METHODS
❖ Pressure plays the role of blood drawing ❖ Wedge or two-slide method
❖ No air contact 1. Push type
❖ Less possibility of contamination 2. Pull type
❖ Materials: ❖ Coverslip/ cover glass method
1. Alcohol swab ❖ Automated method
2. Tourniquet ❖ Sample from:
3. 2 way needle (Multisample 1. EDTA- anticoagulated blood
needle) - Multiple slides
4. Adapter or tube holder - Made within 4 hrs
5. Evacuated tube 2. Capillary Blood
6. Gauze - Few slides
7. Micropore - Made immediately
❖ Materials:
GAUGE
1. Lead pencil
❖ 14: Olive 2. Clean glass slides
❖ 15: Amber - Film slide
❖ 16: Gray - Pusher or spreader
❖ 18: Green

1
PHLEBOTOMY
Hematology 1 (Laboratory)
BS Medical Laboratory Science | Abella, Pelis Florbien A. | SEM 1 2023-2024

FACTORS AFFECTING QUALITY OF

SMEAR

SHORT/THIC LONG THIN

K

Pressure ↓ ↑

Angle ↑ ↓

Spead ↑ ↓

Drop of blood ↑ ↓

CHARACTERISTICS OF A WELL-MADE
SMEAR
❖ ⅔ or ¾ length of slide
❖ Feathered edfe
❖ Lateral edges (margin)
❖ Smooth without waves, holes, or streaks
❖ Rainbow appearance