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L70243200 V2 Turbo Service Manual
L70243200 V2 Turbo Service Manual
VARIANT ™ II
TURBO
Hemoglobin
Testing
System
January 2004
L70243200
VARIANT ™ II TURBO Hemoglobin Testing System
Bio-Rad Laboratories
Diagnostic Group
4000 Alfred Nobel Drive
Hercules, California
94547
USA
CAUTION
Please read through and familiarize yourself with the contents of the service manual.
All personnel using the VARIANT ™ II TURBO should wear standard laboratory safety
apparatus. (e.g. safety glasses, laboratory coat and gloves)
All reference material (i.e. standards and controls) and patient samples should be
considered as biohazardous material and must be handled with caution.
Waste material contains sample waste plus reagents: treat and dispose of as directed by
your laboratory safety guidelines or by local ordinance.
Table of Contents
1 Introduction 1-1
1.1 Introduction...................................................................................................... 1-1
1.2 System Modifications ...................................................................................... 1-2
1.3 Safety Precautions............................................................................................ 1-3
1.3.1 Electrical.............................................................................................................................. 1-3
1.3.2 Mechanical .......................................................................................................................... 1-3
1.3.3 Biohazardous ....................................................................................................................... 1-3
1.3.4 Safety Procedures ................................................................................................................ 1-4
2 Specifications 2-1
2.1 Sampling Station Physical and Electrical Specifications................................. 2-1
2.2 Sampling Station Component Specifications................................................... 2-1
2.2.1 Regulatory Compliance ....................................................................................................... 2-5
2.3 Chromatographic Station Physical and Electrical Specifications.................... 2-6
2.4 Chromatographic Station Component Specifications...................................... 2-6
2.4.1 Regulatory Compliance ..................................................................................................... 2-11
2.5 Minimum Computer Specifications............................................................... 2-12
7 Maintenance 7-1
7.1 Materials .......................................................................................................... 7-1
7.2 VCS.................................................................................................................. 7-2
7.3 VSS .................................................................................................................. 7-5
7.4 Performance Verification................................................................................. 7-8
7.5 VARIANT II TURBO Maintenance Checklist................................................ 7-8
8 Troubleshooting 8-1
8.1 Chromatography Review ................................................................................. 8-1
8.2 Variant Hemoglobin Chromatography ............................................................ 8-3
8.3 Retention Times ............................................................................................... 8-9
8.4 Area Counts ................................................................................................... 8-13
8.5 Error Codes .................................................................................................... 8-15
8.5.1 VCS Error Codes ............................................................................................................... 8-15
8.5.2 VSS Error Codes ............................................................................................................... 8-18
8.5.3 General Error Codes .......................................................................................................... 8-21
8.6 Self Diagnostics ............................................................................................. 8-22
8.6.1 VCST Self Diagnostic Sequence and Flow Chart ............................................................. 8-22
8.6.2 VSST Self Diagnostics Sequence and Flow Chart ............................................................ 8-24
8.7 Timing Chart.................................................................................................. 8-27
8.8 Detector Troubleshooting .............................................................................. 8-28
Appendix A A-1
Fluidic Diagrams ....................................................................................................... A - 1
Table of Contents: ........................................................................................................................... A - 1
VCS Tubing Diagram.................................................................................................................. A - 3
VSS Tubing Diagram .................................................................................................................. A - 5
Appendix B B-1
Mechanical Drawings................................................................................................ B - 1
Table of Contents: ............................................................................................................................B - 1
VCS
VSS
Appendix C C-1
Electronic Diagrams and Layer Drawings ................................................................ C - 1
Table of Contents: ............................................................................................................................C - 1
VCS
VSS
List of Figures
Section 1
Section 3
Section 4
Section 5
Section 6
Section 8
1 Introduction
1.1 Introduction
Introduction
required for sample analysis. There are also reliability upgrades that are required on both
modules. Refer to Section 1.2 for a list of these changes. The VARIANT II TURBO is
shown in Figure 1-1.
Figure 1-1:
The VARIANT II
TURBO Hemoglobin
Testing System
The VARIANT II TURBO Service Manual is intended for Bio-Rad or other appropriately
trained service personnel, as a tool when troubleshooting or performing maintenance on
the VARIANT II TURBO Hemoglobin Testing System.
The Spare Parts section contains a pictorial list of parts required to upgrade a VARIANT II
to VARIANT II TURBO and also contains reliability parts for both systems.
Changes to the hardware in both the VSS and VCS are required for proper sample analysis
using the VARIANT II TURBO HbA1c program. These include the replacement, or
addition of the following parts:
TURBO SPECIFIC
VCS
• Column Heater Block
• Pre-heat Tubing Coil
Introduction
• Cartridge Holder
• Mixing Coil Tubing
VSS
• Sample Needle
• Needle Cover
• Dilution Chamber
• Tube Holder Driver PCB (# 293)
• XYZ Connect PCB (#294-254-1)
• Z Sensor PCB (#294-255-7)
• Z Axis PCB (#294-254-4)
• Z Axis Cable 1, 20 pin
• Z Axis Cable 2, 5 pin
RELIABILITY UPGRADES
All other remaining hardware is unchanged from the original VARIANT II system.
Instructions for upgrading to a VARIANT II TURBO can be found in the VARIANT II
TURBO Upgrade Instructions (L70242100), which is included with the upgrade kit.
1.3.1 Electrical
The main electrical hazard on the VARIANT II TURBO is the primary AC wiring. The
primary AC wiring is located at the back of the chromatographic station and on the right
side of the sampling station. This wiring is used to provide power to the DC power
supplies of each unit and carries a voltage anywhere from 100 v to 240 v. The wiring after
the power supplies carries 24 Volts DC or less and does not represent a shock hazard.
Nevertheless, always treat ANY wire as if it carried a hazardous voltage.
Introduction
1.3.2 Mechanical
1.3.3 Biohazardous
The primary function of the VARIANT II TURBO is the analysis of blood samples. Care
must be taken when the instrument is serviced to prevent exposure of service personnel to
infectious agents. The decontamination procedure, found in the VARIANT II TURBO
Hemoglobin Testing System Operation Manual, MUST be followed prior to servicing the
instrument. The Sample Needle MUST be removed to avoid puncture when servicing the
Sampling Station.
Personal Protective Equipment (PPE) is required for all personnel working on the
VARIANT II TURBO. Safety procedures include:
1. Remove wristwatches and jewelry to better facilitate hand washing and prevent the
puncture of disposable gloves. Double gloving is highly recommended when handling
infectious or potentially infectious material. Change gloves if they become cut or
punctured to prevent skin exposure to infectious material.
2. Completely protect any cut, abrasion or rash on the skin before working with potentially
infectious material. Seal with a waterproof bandage under protective clothing. Do not
Introduction
handle potentially infectious material if the exposed injury cannot be completely sealed.
3. Laboratory coats (cloth or disposable), gowns, smocks or uniforms must be worn while
in the laboratory or clean up room. Remove the protective clothing if visiting a "clean"
area of the laboratory (offices, cafeteria, etc.). Disposable lab coats should be disposed
of in a biohazard container before permanently leaving the work area.
4. Wash hands thoroughly with soap and water after working with any potentially
infectious agent or equipment before leaving the work area, and especially if skin is
exposed to an infectious agent.
5. Keep hands away from mouth, nose and eyes. Avoid touching the eyes while working,
as it is a common route for infectious agents to enter the body. Safety glasses with side
shields are required, especially for individuals who wear contact lenses. Goggles or
face shields are required if there is a potential for splashing.
6. Do not use gloved hands to write, answer the telephone, turn on a light or touch
anything that ungloved personnel may handle. Only materials needed for immediate
procedures should be in the vicinity of the work area. Do not expose books or other
materials that cannot be properly decontaminated.
2 Specifications
2.1 Sampling Station Physical and Electrical Specifications
Humidity 10 - 90%
Specifications
100 to 240 volts AC
50/60 Hz
10 sample capacity
Barcode Reader
Liquid Handling
Specifications
specific)
Secondary Diluent Volume: 431 to 470 µL (Assay
specific)
Sample Needle
Length 122 mm
Specifications
5.0 x 3.0 mm (joint through PV7 to T-
fitting)
7.0 x 4.0 mm (Joint to T-fitting)
8.0 x 5.0 mm (union to external port)
EMC
Weight - Uncrated
(approx.) 33 kg (73 lb)
Humidity 10 - 90%
50/60 Hz
Detector
Baseline Noise < 200 µV peak to peak (10 minute test period
after 30 minute warmup)
Baseline Drift < 1.0 mV per hour (one hour test period after 30
minute warmup)
Specifications
Accuracy + 2%
Precision + 1.0%
Test Condition for QC Flow Rate 2.0 mL/min with 270 µL Dynamic
Mixer and backpressure of 100 kg/cm2
Coil Mixer
Volume 360 + 10 µL
Pressure Sensor
Degasser
Vacuum Error Detector Allowable Range = + 100 hpa of the set level
Specifications
Loop Size 23 µL
Cartridge Holder
Size 18 mm O.D. x 62 mm Length (74 mm fitting
to fitting)
Accuracy + 1o C
Stability + 0.5o C
Pre Heater Tubing Coil 1/16 “ O.D. x 0.25” I.D. x 610 mm long Stainless
Steel
Specifications
port) O.D./I.D.: 1/8 inch x 3/16 inch
EMC
UL 3101-1/10.93
Memory 128 MB
USB Ports 2
Mouse PS2
1. Choose a location that is away from direct sunlight and is relatively dust free.
2. Room temperature should be between 15 and 30º C (60-85ºF).
3. The bench or table should have a flat, level surface that is free from vibrations and is
capable of supporting up to 115 kg (about 250 lb).
NOTE: All four feet of the VSS MUST be on the counter. If the surface is not
deep enough to accommodate the front feet, obtain removable footpads from a
local hardware or home improvement store. Secure them under the VSS, one
on each side, far enough back so they allow the module to be level and secure
on the counter.
4. A grounded electrical receptacle should be within 1.8 meters (6 feet) of the system.
5. The maximum power consumption is 1750 VA (VCS, VSS, CPU, monitor and
printer).
6. The Personal Computer (PC) should be located as close to the VARIANT II TURBO
as possible to provide adequate room for communication connections.
The VARIANT II TURBO will arrive in three separate boxes; one for the VSS, one for the
VCS and one for the Accessories. The PC, monitor and printer will also be in separate
boxes. A Customer Acceptance Form and System Warranty Card should be completed by
Installation
the customer and returned to Bio-Rad Laboratories after inspection of the system.
Accessories
Remove all accessories from the box and verify that all components are present. The
following is a list of all accessories supplied at installation:
VARIANT II
1. Remove the ties surrounding the two larger boxes. Lift the top box off of each base.
Installation
Figure 3-2:
Unpacking
the VCS
Figure 3-1:
Unpacking the VSS
2. Remove the protective bags from around the VCS and VSS.
3. Each module is heavy, requiring at least two people to lift. Carefully lift the VCS
onto the bench top and position it so that all of the rubber feet are squarely in
position. Repeat with the VSS, PC and printer. Remove the monitor and place it on
top of the PC.
4. Remove the needle cover and front bezel cover from the VSS. Disconnect and save
the sampling arm shipping bracket. Make sure the sample probe is connected
properly. Replace the front bezel cover and needle cover.
1. Connect one end of the reservoir cable to the Reservoir Unit and the other end to the
back of the VCS. Place the reservoir unit on the VSS behind the right belt.
2. Connect the power cords to the VSS, VCS. PC, monitor and printer. Plug all power
cords into power outlets.
Turn on the PC. Start CDM from the icon on the desktop. In the SETUP/Configuration
screen verify that the instrument type is VARIANT II. Set the communication ports for the
VCS (gradient module) and VSS, for Instrument 1, based on placement at the back of the
PC. The system must be in Inactive state to make changes to the port settings.
Installation
The HP Compaq D530 series computer requires the use of a multi-line communication
cable. This line replaces the 4 port rocket board from previous computers supplied with
the VARIANT II. The end of each cable is a DB-9 male connector requiring the use of the
RJ-45 to DB-9 cables supplied with the system. Figure 3-3 shows the communication port
numbers on the back of this PC.
4 5 6 7
1 2
1. Remove the caps from the waste tank. Remove the inner seal from the main cap.
2. Insert the terminal end of the waste tank sensor cable through the main cap. (Figure
3-4)
Installation
3. Place the waste sensor into the tank and screw the main cap over the sensor until it is
tight. Connect the waste tank signal cable to the terminal labeled WASTE on the
back of the VCS.
4. Create a small vent hole in the secondary cap and install the cap on the waste tank.
5. Remove the caps from the barbed fittings for waste at the back of the VCS and VSS.
Retain the caps.
6. Connect one silicon tube to the Waste port at the back of the VSS. Connect the other
silicon tube to the Pump Drain port at the back of the VCS. Connect both to the
waste tank inlet. The tubing should have sufficient length to reach the waste tank on
the floor. Cut if necessary.
7. Connect the remaining Tygon tube to the Drain port at the back of the VCS. Connect
the opposite end to the waste tank inlet. The tube should have sufficient length to
reach the waste tank on the floor. Cut if necessary.
8. Place the waste tank in a position so the tubing is sloped downward at all times.
1. Remove all inlet port plugs from the reagent and sample ports at the back of the VCS
and VSS and save them.
2. Connect the finger-tight fittings for Buffers A, B and Wash/Diluent tubing to the
appropriate ports on the VCS and VSS. The caps of the buffer lines are marked
accordingly. Make sure the fittings are tight and are not cross-threaded.
3. Connect one end of the sample line at the back of the VSS and the other at the back
of the VCS.
4. Unpack the buffer and wash bottles from the application to be installed. Remove the
caps and replace with the corresponding buffer and wash lines. Make sure the
solvent filter is at the bottom of the bottle and the caps are tight. DO NOT grasp the
tubing below the cap or the solvent filter with bare hands as it may cause
contamination of the buffer.
5. Place the buffer and wash bottles in their appropriate positions on the reservoir unit.
1. Open the cover to the VCS. Locate the column heater in the center of the VCS. Turn
the latch and open the door.
2. Remove the cartridge holder from the clip at the top. (Figure 3-5)
Installation
Figure 3-5:
Cartridge
Holder
3. Unscrew the top of the cartridge holder and remove the dummy cartridge. Insert a
new analytical cartridge from the reorder pack, making sure the arrow on the
cartridge is pointing in the same direction as the arrow on the cartridge holder.
Replace the top of the cartridge holder.
4. Unscrew the bottom of the cartridge holder and remove the dummy cartridge. Insert
a new guard cartridge from the reorder pack, making sure the arrow on the cartridge
is pointing in the same direction as the arrow on the cartridge holder. Replace the
bottom of the cartridge holder.
5. Place the cartridge holder back into the clip of the column heater. Close the column
heater door and secure it with the latch.
Prior to shipment the fluid lines within the VCS are filled with a very weak concentration
of phosphoric acid (0.01%). Prime the lines before the first operation to remove all fluid
and air bubbles that may be trapped.
1. Turn the VCS power on. The system will perform a short self-check.
2. Turn the VSS power on. The system will perform an initialization.
3. In CDM select the Instrument 1 icon at the top left of the screen. Select Return to
Active in the status window. When prompted "Do you want to perform the
automatic warmup operation" select No. The instrument will go into Ready state.
Close the status window by selecting OK.
NOTE: If either the VARIANT or VSS communication status, in the status window,
says "Not OK" in red, then the communication port for that module may not be set
correctly. Close the status window by selecting OK. Select MAINTAIN/Instrument
and select Make Inactive at the bottom of the screen. Select No when asked about
performing a Shutdown. Select SETUP/Configuration and set the communication
ports to the correct settings. Repeat Step 3. If there is still no communication verify
that the cable is inserted correctly.
4. Remove the 25 mL syringe from the accessories box. Place the syringe securely into
either one of the two pump ports. Open the port 1/2 turn counterclockwise.
5. Slowly pull back on the syringe plunger until it is filled to 25 mL. Close the pump
port, remove the syringe and dispose of the fluid.
6. Repeat Steps 4-5 until no bubbles are seen coming from the port.
7. Repeat Steps 4-6 for the other pump port.
1. Remove the 10 mL syringe from the accessories box. Fill the syringe with 10 mL
deionized water.
2. Securely insert the syringe into the piston/seal wash port. Slowly inject the water into
the port.
3. Remove the syringe.
System Flush
A system flush of all fluid lines is required prior to first operation. The short flush will
take 20 minutes to complete and flushes the buffer and wash lines in both the VCS and
Installation
VSS.
1. Select SETUP/Test in CDM. Select the down arrow next to the field Select New
Test and change the test to the application to be run. Acknowledge the message
about making sure the correct reagents and cartridge are in place.
2. Select either the cartridge or reagent radio button. Select Start System Flush at the
bottom of the screen. Select Short flush then OK to begin the flush.
3. When the flush is complete select the Instrument 1 icon at the top left of the screen.
Select Return to Ready. When the instrument status changes to Ready select OK to
close the status window.
NOTE: The 7 port valve will stay in purge position until the system is returned to
Ready state.
1. Open the MAINTAIN/Instruments window in CDM 3.6T. Locate the box labeled
Pump. Check the pressure on Pump A by entering 0 in the %B field. Set the Flow
Rate at the rate defined for the installed cartridge. Refer to the SETUP/Test/ Edit
Test screen for the appropriate test to find the proper flow rate. Leave the Upper and
Lower Pressure Limits at the default settings. Set the Flow Timer to 5 minutes.
Figure 3-6 shows an example of the pump settings.
2. Open the MAINTAIN/Instruments window in CDM 3.6T. Locate the box labeled
Pump. Check the pressure on Pump A by entering 0 in the %B field. Set the Flow
Rate at the rate defined for the installed cartridge. Refer to the SETUP/Test/ Edit
Test screen for the appropriate test to find the proper flow rate. Leave the Upper and
Lower Pressure Limits at the default settings. Set the Flow Timer to 5 minutes.
Figure 3-6 shows an example of the pump settings.
Figure 3-6:
Pump Settings
Installation
3. Open the front door of the VCS and the column heater.
4. Select Start Flow. Let the pump run for 2-3 minutes and then observe the pressure
reading at the bottom of the screen. The pressure should be stable with a range of +/-
5% from the mean pressure. If unstable, refer to the following section on removing
air from the pumps.
5. Check for leaks at all stainless steel fittings in the VCS, especially around the
cartridge holder during the remaining time.
6. Check Pump B by changing the %B field to 100. Repeat Step 3 and 4.
7. Close the column heater and front door of the VCS.
Unstable pressure, with no leaks in the fluid path, indicates the presence of air in the pump.
The following procedure should be used to eliminate air from all parts of the unstable
pump.
1. Using the 8 mm and 10mm wrenches, disconnect the stainless steel nuts from the
outlet check valves at the top of the pump. Use the 10 mm wrench to hold the check
valve in place while turning the nut counterclockwise with the 8 mm wrench.
Completely remove the nut and ferrule from the fitting.
2. Open the MAINTAIN/Instruments screen in CDM 3.6T. Enter the appropriate
setting for the pump in the %B field (0 for Pump A, 100 for Pump B). Set the flow
rate to 3.0. Leave the default settings for Upper and Lower Pressure Limit. Set the
timer for 1 minute.
3. Place an absorbent cloth or paper towel under the pump. Select Start Flow. Let the
pump run for 1 minute.
4. Using the wrenches reconnect the outlet lines to the check valves being careful not to
cross thread or over-tighten the fitting. Use a tissue or paper towel to remove all
fluid from around the fitting.
5. Set the flow rate to the flow rate defined for the installed cartridge. Change the timer
setting to 10 minutes. Start the flow. Check for leaks, while the pump is running, at
the outlet check valves by holding a tissue or other thin, absorbent material up to the
fitting. Tighten the fitting further if liquid is absorbed onto the tissue. Check the
pump pressure at the bottom of the screen after the pump has run almost 10 minutes.
If the pressure is still unstable, repeat steps 1-4 above.
Configuration Fields
Installation
Edit the following fields, according to user preference, in SETUP/Configuration (Figure 3-
7) while the system is in Ready state:
• System Name - usually includes the serial number of the instrument
• Date Format and Separator - default is Month/Day/Year
• Unity ID and PC Export Path - for members of the Unity program
• LIMS Communication Port - define a port other than 0 only if connected to a
LIMS system
• Auto Import from LIMS - select to change from Off to On; used for
downloading additional patient information from LIMS
• Instrument Name for Instrument 1 - usually site name
• Instrument Name for Instrument 2 - only if 2 systems connected to PC
• Auto Report Printing - check to print all data and reports during a run
Microvial Adapters
1. Remove the microvial adapters and barcodes from the accessories box.
2. Label the adapters with the appropriate barcodes for the application(s) to be used.
Hardware Checks
Some parts of the VCS and VSS may need to be checked at time of installation.. These
include:
Refer to the appropriate part of Section 5 of the VARIANT II Service Manual for details of
the procedures.
Installation
3.4 De-Installation
NOTE: Prior to shipment decontaminate the sample fluid pathway and clean the
sample needle. Refer to the VARIANT II TURBO Operation Manual for the
procedures.
Pallet
5-6 Plastic Bags (assorted sizes)
Plastic Coverings (VCS and VSS)
Package Sealing Tape
Strapping material
Plastic wrap
Boxes
VCS (with wooden pallet)
VSS (with wooden pallet)
Monitor
PC (with box for keyboard and mouse)
Printer
Accessories
Reservoir Unit (padded)
1. Remove the buffer and wash bottle lines from their respective bottles. Place the lines
into a container filled with deionized water placed on top of the VSS (not on the
bottles). Cap the buffer and wash bottles but do not remove them from the reservoir
tray.
2. Perform a Short System Flush. Refer to Section 3.3 - System Flush for the
procedure.
Installation
lines completely and blot the solvent filters on a lint free cloth. Insert the port plugs,
in place at install, into all buffer and wash ports. Place the lines in a plastic bag for
shipment.
10. Disconnect the waste lines from the back of the VCS and VSS. Drain the lines into
the waste container. Disconnect the lines from the waste container and place them
into a plastic bag for shipment. Place the waste port caps, present at install, on the
waste ports at the back of the VCS and VSS.
11. Disconnect the waste level sense cable from the back of the VCS. Remove the main
cap from the waste container and thread the cable through until it clears the cap.
Replace the main cap on the waste container. Place the cable in a plastic bag for
shipment.
12. Remove the secondary cap of the waste bottle. Empty the waste into a sink
designated for biohazard waste. Rinse the empty container with household bleach
followed by deionized water. Place the waste bottle in a plastic bag for shipment.
13. Remove the reservoir tray from the VSS and place in the foam padded shipping box.
Place the box into the accessories box.
14. Place the waste container along with all the tubing lines and cables from the VCS and
VSS into the accessories box. Secure the top of the box with package sealing tape.
15. Remove the needle cover and front bezel cover from the VSS. Re-install the
sampling arm shipping bracket that was removed at installation. Replace the covers
and secure them with tape.
16. Secure the front cover of the VCS with tape.
17. Place the VCS and VSS pallets on the main shipping pallet. The VCS and VSS
require two people to lift them from the counter. Place the VCS and VSS on their
respective pallets. Wrap each with the plastic covers then place the box covers over
the modules. Secure the top to the bottom of each with straps.
18. Disconnect the keyboard, mouse, printer and monitor cables from the back of the PC.
Disconnect the printer cable from the printer. Place all cables in a plastic bag for
shipment. If the shipment is going by air, the toner cartridge should be removed from
the printer and bagged separately.
Installation
19. Disconnect the power cords from the monitor, PC and printer. Place the cables in a
plastic bag for shipment.
20. Place the monitor, printer and PC in their respective boxes. Place the keyboard and
mouse in a box and place inside the box with the PC.
21. Place the cables and power cords from the PC, monitor and printer in the box with
the PC. Secure the top of all boxes with package sealing tape.
22. Place all boxes on top of the VCS and VSS boxes on the pallet. Secure the boxes
together by wrapping them with plastic wrap.
This section describes the changes to the existing VARIANT II Service Software.
VARIANT II and VARIANT II TURBO use the same service software, but some options
cannot be used with the VARIANT II firmware. Messages will appear on the screen when
the wrong firmware is in place.
4.1 Introduction
Service software is a separate application from CDM 3.6T. It is used to make adjustments
and alignment changes to both the VCS and VSS, and to manually control both modules.
In addition, it logs hardware error codes that indicate where a specific problem exists.
These errors may cause non-specific messages in CDM 3.6T. It is then necessary to access
the service software in order to determine the source of the problem. Refer to Section 7.0
Troubleshooting for a list of the current error codes.
Service Software
Each module has its own service software. The current versions are listed below.
VCS: 1.08
VSS: 3.01
Access either software by selecting the Start button at the bottom of the screen. Select
PROGRAMS/Variant 2 Service and then either VCS 1.08 or VSS 3.01. Both applications
are password protected. Enter the password, all in capital letters, VARIANT2QC when
prompted, after selecting the appropriate software option.
NOTE: CDM 3.6T MUST either be closed or have the instrument(s) in Inactive state
in order to use the COM ports with the service software.
In the service software select the Close button in any open window to close that option.
Multiple windows can be open at any given time. To close the software select the "x" in
the upper, right corner of the Main Menu screen.
There are no changes to the existing VCS service software for VARIANT II TURBO.
Refer to Section 4.2 of the VARIANT II Service Manual for information on the various
screen options.
There are minor changes to the existing VSS service software for VARIANT II TURBO.
Refer to Section 4.3 of the VARIANT II Service Manual for information on the remaining
screens and options.
VARIANT II TURBO
The manual screen has an added option under Nozzle Z for Sensor Position. (Figure 4-1)
Selecting this option will move the sampling arm down to the low sensor position.
VARIANT II
When connected to a VARIANT II, a warning message (Figure 4-2) will appear when the
Sensor Pos option is selected in the Manual screen. This option can only be used with
VARIANT II TURBO firmware.
Once OK is selected for the warning, the Sensor Pos option will be removed from the
Service Software
Manual screen.
Two new fields have been added to the Sensor Status screen: Detail and Z-Low pos.
(Figure 4-3)
VARIANT II TURBO
When the Check Status option is selected from the Sensor Status screen, data appears
in the Z-Low Pos field for the low position sensor.
VARIANT II
When the Check Status option is selected from the Sensor Status screen, the Z-Low
Pos field disappears. (Figure 4-4)
Service Software
A Detail option has been added to the Status Screen for use by both VARIANT II and
VARIANT II TURBO. Selecting this option shows an expanded Status screen with
binary data. (Figure 4-5)
Service Software
Figure 4-5: Sensor Status Details
5 TURBO Procedures
All procedures for the VCS remain the same as in the VARIANT II Service Manual.
There are, however, some minor changes to the Column Heater Adjustment (Section 5.2),
along with the 6 and 7 Port Valve Rotor and Seal Replacement Procedure (Section 7.1.11).
Procedures
5.1.1 Column Heater Adjustment
The following is a change to Section 5.2, Step 9 of the VARIANT II Service Manual.
Allow adequate time for equilibration. The temperature reading must be within 1
degree of the CDM display.
• The diagrams in Figures 5-3 and 5-4 show the correct tubing connections for the
6 and 7 port valves.
Port 4 of 7
Figure 5-3: 6 Port
port valve
Procedures
Valve Connections
Sample Sample
Inlet Line Loop
Waste
Port 2 of the
6 port valve
Dynamic
Mixer
Pump
Coil
Mixer Figure 5-4: 7 Port
Valve Connections
Refer to the VARIANT II Service Manual for VSS alignment procedures. The procedures
for TURBO are the same as for the VARIANT II; however, figures 6-39 and 6-64, of the
Manual screen, are screen captures from the previous version of VSS Service Software.
The change in the Manual screen for the latest version of service software does not affect
the procedures that reference these two figures.
Procedures
6 Replacement Procedures
The following procedure from the VARIANT II Service Manual has been modified for
TURBO:
Replacements
All other VCS replacement procedures remain the same as in the VARIANT II Service
Manual.
The column heater block for TURBO contains a pre-heat tubing coil.
Follow the procedure in Section 7.1.6 of the VARIANT II Service Manual for installation
of the new column heater block. Note the following changes to the procedure.
• Remove the pre-heat coil from the old column heater before removing it from the
column heater unit.
• Re-install the pre-heat coil into the new column heater after securing it to the
column heater unit.
All VSS replacement procedures remain unchanged from the VARIANT II Service
Manual, except Figure 6-1 below replaces Figure 7-73.
Replacements
Section 6.2.1 below describes the replacement of the new tube hold down assembly
sensor and wiring.
Figure 6-2 shows the components of the tube hold down sensor and wiring upgrade kit.
1. Wiring Assembly
2 2. Cable with Sensor
5
Bracket Assembly
3. Sensor Assembly
4. Screw Sets A (M3X6)
4 and B (M3X6-Stainless
Steel)
5. Tie Wraps
Replacements
1
3
Figure 6-2: VSS Tube Hold Down Sensor and Wiring Upgrade Kit
1. Turn off the power to the VSS and remove the power cord.
2. Remove the tube hold down assembly.
3. Remove the old wiring along with the photo sensor assemblies. (Figure 6-3)
4. Turn the lead screw manually until the block is at the bottom position. (Figure 6-4)
To the bottom
5. Secure the sensor bracket of the new cable assembly using the 2 stainless steel screws.
(Figure 6-5)
1. Cable
2. Sensor Bracket
3. Stainless Steel
Screws
3
1
6. Secure the sensor unit using the 2 screws (M3X6). (Figure 6-6)
1. New Sensor
Assembly
2. M3X6 Screws
Replacements
2
7. Insert the end of the cable to the connector #5322. Push the connector housing to lock
the cable in place. (Figure 6-7)
Push
Insert
Cable
Replacements
bracket
Two screws
11. Connect the new wiring assembly at #5320 on the sensor board. Place a tie wrap around
both the new wiring and the motor wiring. (Figure 6-10)
Replacements
Figure 6-10: Installing the New Wiring Assembly
7 Maintenance
This section contains the minimum requirements for performing annual preventive
maintenance on the VARIANT II TURBO. Any additional maintenance items can be
performed based on regional requirements. Refer to the VARIANT II TURBO Operation
Manual for daily, monthly and periodic maintenance procedures.
NOTE: This section assumes the system requiring maintenance has all of the
Maintenance
reliability upgrades to the hardware, current firmware and software.
7.1 Materials
Required Materials
*Refer to the VARIANT II TURBO Maintenance Report in Section 7.5 for additional parts
that may need replacement. The part numbers can be found in Section 10 of the
VARIANT II Service Manual.
7.2 VCS
Safety
Turn off the VCS and remove the power cord from the back of the unit.
Detector
1. Clean the flow cell. Follow the package insert provided with the flow cell cleaning
Maintenance
kit. Replace the flow cell only as needed. Refer to VARIANT II Service Manual
Section 7.1.7 Detector/Flow Cell for the procedure.
2. Check the inlet and outlet tubing connections for leaks or damage. Clean and repair
as needed.
3. Check the lamp alignment. Refer to VARIANT II Service Manual Section 7.1.7
Detector/Lamp Assembly for details.
4. Check the detector board. Do NOT adjust the pre-amplifier outputs unless you are
prepared to perform a detector noise and drift test. Refer to VARIANT II Service
Manual Section 5.9 Detector Board Adjustment and Section 8.8.1 of this manual for
the procedures.
Degasser
Check the tubing connections for leaks. Clean and repair as needed.
Pumps
Refer to VARIANT II Service Manual Section 7.1.5 for pump component replacement
procedures.
1. Remove the pump tray. Refer to VARIANT II Service Manual Section 7.1.5 Pump
Unit/Motor Photo Sensor for details of the procedure.
2. Replace pistons, seals and bushings. Check valves, springs and other pump
components should only be replaced if defective.
3. Remove the front U section of the pump chassis. Remove the pump cam. Remove
the top 2 set screws, loosen the bottom 2 set screws, hold the back of the motor shaft
with an adjustable wrench and twist the cam off. DO NOT PRY IT OFF. If the cam
was difficult to remove, smooth off the shaft before replacing the cam.
4. Inspect crosshead rods. Clean and lubricate (light film of lithium based grease) if
contaminated. If the crosshead is scored it must be replaced along with a new linear
bearing. DO NOT remove the guide pin holder unless absolutely necessary.
5. Clean the thrust bearing and washers. Pack the thrust bearing and coat the washers
with a molybdenum-based grease.
6. Reinstall the pump cam. Restore it to the original position so an additional pockmark
will not be placed on the shaft. Make sure that the set screws over the flat side of the
shaft are centered on the flat and that the cam is fully seated against the pump chassis
prior to tightening the set screws.
7. Reassemble the pump.
Replace the rotor and stator face. Flush out the surrounding tubing if the rotor
Maintenance
shows disintegration.
NOTE: The rotor and stator face of the 7 port valve is only replaced or cleaned as
needed.
Syringe Assembly
1. Clean and then lubricate the lead screw with molybdenum-based grease.
2. Clean and then lubricate the guide rods with a light film of lithium-based grease.
3. Clean the syringe barrel and tip. Replace the syringe plunger if the tip is damaged.
4. Inspect the drive belt and replace if damaged.
5. Clean the optosensor with compressed air.
6. Check the fittings at the 3-way valve for leaks or damage. Clean and repair as
needed.
Cartridge Holder
1. Clean the inside with deionized water and a swab. Rinse well. Sonicate in deionized
water if possible to remove all dried residue. Replace the holder if the threads are
damaged.
2. Inspect the tubing to the detector. Replace if damaged or plugged.
NOTE: The cartridge to detector tubing can also be replaced annually based on
regional requirements.
Tubing
Cleaning
Miscellaneous
The components listed below should be inspected. Clean or replace all items as needed.
Break-in
7.3 VSS
Safety
Dilution Chamber
1. Remove the dilution chamber and clean it thoroughly using household bleach. Rinse
Maintenance
thoroughly with deionized water.
2. Fill the syringe from a Flow Cell Cleaning Kit with deionized water and attach the
syringe, with the fittings, to one of the 3 threaded ports on the right side of the tower.
Push the water through the port. Repeat with the remaining ports.
3. Check all fittings for leaks and repair if needed.
4. Inspect Pinch Valves 3 through 7. Replace if damaged.
5. Inspect all tubing from the dilution chamber to the internal waste tank. Clean or
replace based on regional requirements. This includes the following:
Silicone Waste Tubing from All Drain Ports through Pinch Valves 3, 4
and 7 to the Internal Waste Tank (P/N 270-2023, 270-2024 and 270-2025)
6. If tubing in the pinch valve was not replaced, reposition the old tubing so the valve
will open and close on a different position.
7. Using the VSS Service Software and a squirt bottle filled with deionized water,
verify that all drain lines evacuate properly. Use the following settings in the Manual
screen:
Syringe Assembly
1. Turn off the power to the VSS and remove the power cord from the side.
2. Clean and then lubricate both lead screws with molybdenum-based grease.
3. Clean and then lubricate the guide rods with a light film of lithium-based grease.
4. Assess the amount of movement in the lead screw. If the movement is excessive
replace the lead screw and flange nut. Refer to the VARIANT II Service Manual
Section 7.1.10 for the procedure.
5. Inspect the drive belts. Replace if damaged.
Maintenance
Clean the internal waste chamber. Inspect the tubing and fittings. Clean or repair as
needed.
Sampling Assembly
WARNING: The sample needle is very sharp. Use caution when handling to avoid injury.
2. Clean and then lubricate the lead screw with molybdenum-based grease.
3. Clean and then lubricate the 4 guide rods with a light film of lithium-based grease.
4. Test the movement of the lead screw. If the movement is excessive replace the lead
screw and flange nut.
5. Inspect the Y and Z drive belts. Replace if damaged.
6. Clean the optosensors with compressed air.
7. Inspect the fitting and tubing at the sample needle. Repair or replace based on
regional requirements (P/N 270-2021)
Maintenance
Rack Transport
1. Clean the 2 front and 4 rear opto and tube sensors with compressed air.
2. Check the rack drives for smooth motion with the power off. Watch for loose
pulleys. Repair if damaged.
3. Clean the left and right conveyor belts with deionized water or isopropyl alcohol.
Alignments
1. Reinstall the sample probe. Attach the power cord and turn on the power.
2. VSS Alignment procedures are found in Section 6 of the VARIANT II Service
Manual. Check the following alignments and adjust if needed
1. Set up a sample run using controls and previously analyzed patient samples.
2. Assess the VSS alignments, dilution performance and pump pressure stability during
the run. Make all required adjustments if any component is not functioning properly.
3. Assess the quality of the chromatogram including sample areas, QC values and
retention times. Correct for any deficiencies.
8 Troubleshooting
This section of the VARIANT II TURBO Service Manual contains flow charts, guidelines
and error codes for troubleshooting the VARIANT II TURBO. Examples of acceptable
chromatography are included as a reference when evaluating baselines, peaks and retention
times.
Troubleshooting
The following peaks are contained in the peak table of the VARIANT II TURBO
Hemoglobin A1c test file:
• A1a
• A1b
• F
• LA1c (labile A1c)
• CHb (carbamylated hemoglobin)
• A1c
• Ao
• Variant Window (contains variant hemoglobins not S or C)
• S
• C
Figure 8-2 through 8-5 show examples of patient samples containing some common
variant hemoglobins in the heterozygous state (patient has Hb A plus the variant). These
examples are reportable Hb A1c results; however, if a Variant, S or C window is seen with
no A1c or Ao peaks present, the sample is homozygous for the variant (no Hb A present)
and the result cannot be reported.
Troubleshooting
Use caution when reviewing results with a Variant, S or C window. Their presence may
also indicate a retention time issue where all, or part, of the Ao peak is coming off too late
and is misidentified or can be carryover from a previous sample containing the variant
(Figure 8-6). If Hb A1c is present, there will always be an Ao peak also present.
True variant hemoglobins will normally be greater than 20%. Refer to Section 8.3 for
troubleshooting retention time issues.
Troubleshooting
Troubleshooting
The current retention times for each assay can be found in the Setup/Test screen in CDM
3.6T.
Troubleshooting
Use the flow chart in Figure 8-8 to troubleshoot early A1c and Ao retention times.
Troubleshooting
Use the flow charts in Figures 8-9 and 8-10 to troubleshoot late A1c and Ao retention times.
Troubleshooting
Refer to the Setup/Sample Types screen in CDM for the acceptable area count
range.
When evaluating total area count issues consider what type of sample is being
affected. Samples that are pre-diluted and mixed properly should always have an
area within the specified range. Area counts out of range consistently for pre-
diluted and primary tubes are related to issues in the common pathway for both
Troubleshooting
samples. Area counts out of range that affect only primary tubes may point to an
issue with the dilution process of patient samples.
Troubleshooting
• Inactive (stop immediately – System stops immediately
Recover: Action by user to recover the system
Troubleshooting
Detection Detector A/D converter has malfunction.
Activity Inactive (stop immediately)
Recovery Check the detector, detector board, flow cell and
perform an autozero.
0x54 Pump Internal data error
Detection Communication problem occurred between Main CPU
and VCS.
Activity Inactive (stop immediately)
Recovery Check cables. Reboot VCS and/or pc.
Troubleshooting
Detection Tube holder can not detect the tube hold position.
Activity Inactive (stop immediately)
Recovery Check the tube holder mechanically and
electronically. Reboot the VSS and verify
movement during initialization.
0x2E Rack Barcode reading error
Detection Barcode reader can not read the barcode data.
Activity Active (Warning only)
Recovery After Error message is send once, it automatically
recovers from the error.
0x2F Rack Front rack carrier home position error
Detection Front rack carrier can not detect the home position
sensor.
Activity Inactive (stop immediately)
Recovery Check the front rack carrier mechanically and
electronically. Reboot the VSS and verify
movement during initialization.
0x30 Rack Front rack carrier movement error
Detection Front rack carrier can not detect the limit position
sensor.
Activity Inactive (stop immediately)
Recovery Check the front rack carrier mechanically and
electronically. Reboot the VSS and verify
movement during initialization.
0x31 Rack Rear rack carrier home position error
Detection Rear rack carrier can not detect the home position
sensor.
Activity Inactive (stop immediately)
Recovery Check the rear rack carrier mechanically and
electronically. Reboot the VSS and verify
movement during initialization.
0x32 Rack Rear rack carrier movement error
Detection Rear rack carrier can not detect the limit position
or the stop position sensor.
Activity Inactive (stop immediately)
Recovery Check the rear rack carrier mechanically and
electronically. Reboot the VSS and verify
movement during initialization.
Troubleshooting
TET Loading Error. Invalid TET sent by CDM will cause moving
0x53 to a TET_NONE state not before sending at least one status
message with TET_LOADING state.
0x60 EEPROM load/write error
The system will go through an initialization sequence upon power up. If any part of this
self diagnostic fails, an error message will be generated in CDM.
The following sequence is performed at power up. If an error occurs, a message is sent to
Troubleshooting
1. RAM check. Data is written and read in RAM area. If an error occurs, an audible
alarm will sound. In this case, a report will be not be sent to CDM.
2. Syringe movement check. Syringe moves down and then up to the home position.
Home position sensor output is checked.
3. 6-Port valve unit movement check. 6-port valve rotates from Load to Inject, and
position sensor output is checked.
4. 7-Port valve unit movement check. 7-port valve rotates to Purge from Dynamic,
and next to T-mixer position. Position sensor output is checked.
5. FAN movement check. The moving signal from the fan is checked.
6. Pump unit movement check. Pump A and B move at flow rate of 1 mL/min for 10
seconds, and movement sensor output is checked.
7. Dynamic mixer movement check. Dynamic mixer moves, and movement sensor is
checked.
Power On
RAM Check
(Communication Begins)
Troubleshooting
(HW_BUSY)
Syringe Down (1800 pulses)
NG
NG
Syringe Home
NG
VCST sends error code to CDM
6 Port Load Position
NG
NG
Pumps Stop
NG Figure 8-11:
VCST Self
Diagnostic
Dynamic Mixer Check
Flow Chart
NG
(TET NONE)
The system will go through an initialization sequence upon power up. If any part of this
self diagnostic fails, an error message will be generated in CDM. For memory errors, the
VSST will sound an alarm and will not be able to communicate a message to CDM.
Figure 8-12 shows the flowchart for this sequence.
1. RAM check. Data is written and read in RAM area. When an error occurs, an audible
alarm will active. In this case a report will be not being sent to CDM.
Troubleshooting
2. ROM check. Checks the checksum that is stored in the EEPROM. When the stored
checksum and the recalculated checksum are different, an audible alarm will active. In
this case, a report will not be sent to CDM.
3. Spin head movement check
• The spin head goes down and up, and then the home position sensor output is
checked.
• Check if both the home sensor and the hold sensor exist in the same time.
• Check the moving pulses when it goes to home.
When a malfunction of the spin head is detected, a status will report to CDM.
4. Rack rear carrier movement check. The rear carrier goes to home, spinning, sampling
and limit positions, and then returns to home position. In each position, the sensor
output is checked. When a malfunction of the rear carrier is detected, an error will
report to CDM.
5. Rack front carrier movement check. The front carrier goes to home and to limit
position. In each position, the sensor output is checked. When a malfunction of the
front carrier is detected, an error will report to CDM.
6. Barcode reader check. The barcode reader turns itself on and then sends the result to
VSS main CPU, and then the result is checked. When a malfunction of the reader is
detected, an error will report to CDM.
7. Right and Left Conveyer, and Rack sensors check.
• Left Conveyer moves to rear and then checks Front-Left rack sensor.
• Right Conveyer moves to rear and then checks Front-Right rack sensor.
• Left Conveyer moves to front and then checks Rear-Left rack sensor.
• Right Conveyer moves to front and then checks Rear-Right rack sensor.
When a malfunction of the rack sensors or of the conveyers is detected, an error will
report to CDM computer.
8. Tube holder movement check.
• The tube holder goes down and up, and then the home position sensor output
is checked.
• Check if both the home sensor and the hold sensor exist in the same time.
• Check the moving pulses when it goes to home.
Troubleshooting
When a malfunction of the Z axis is detected, an error will report to CDM.
When a malfunction of the syringe unit is detected, an error will report to CDM.
If any check fails, the system goes back to the Restart point and begins the sequence again.
If it fails after 3 tries, the system sends an error code to CDM and a message is displayed.
Power ON
RAM Check
Error: Alarm Only
EEPROM Check
Error: Alarm Only
Troubleshooting
Restart Restart
NG NG
NG
NG NG
NG
Figure 8-13 shows a combined timing chart for the TURBO VCS and VSS.
Troubleshooting
Baselines on all chromatograms should be relatively flat and the tracing should be smooth.
If they are not, check the following:
The flow chart in Figure 8-14 can be used if neither of the above are found to be the cause
of the problem.
Troubleshooting
Section 8.8.1
Figure 8-14:
Detector Figure 8-21
Troubleshooting
Flow Chart
The Detector Noise Test is a field service diagnostic tool that is used to rule out detector
issues when troubleshooting. This procedure is also applicable to the VARIANT II
System.
system.
Required Materials
NOTE: The Noise Test Spreadsheet is available from CSD Technical Support
or your regional support group.
1. Follow the procedure in the Flow Cell Cleaning Kit to thoroughly clean the flow cell.
It is not necessary to re-clean the flow cell if it was done on the same day for a
Detector PCB adjustment.
2. Open CDM. Select SETUP/Test. Change the test to Noise for VII or Noise_T for
TURBO. Acknowledge the message about installing new buffers and cartridge.
Select the radio button for cartridge and then buffers and verify that each has a line in
use.
NOTE: If GHb cartridge and buffers are to be used, the NoiseGHb tss file must
be used. It is available from CSD Tech Support or your regional technical
support group and must be copied into the CDM database using
D:\CDM35\Copytest.exe. Make sure an unused test is selected in CDM before
copying the NoiseGHb test to avoid corrupting the current test lot number
information.
3. Install a cartridge in the cartridge holder. If running the NoiseGHb test, use an old
GHb cartridge. A new GHb cartridge may get damaged while running the noise test
due to the high flow rate.
4. Open the MAINTAIN/Instruments screen and prime Buffer B (100% B) at a flow
rate of 2.0 mL/min for 5 minutes.
5. Observe the Light Level reading in the Detector field of the screen while Pump B is
running. The reading should be approximately 0.02V. Select Zero 20mV on the
screen to zero the detector. If the light level reading drifts down from 0.02V repeat
Zero 20mV until the level stabilizes.
6. While Pump B is running, select the Monitor option at the top of the screen. Select
Start and adjust the scaling so that the screen shows the detector output voltage +
0.002 V. Monitor the baseline to verify that it is flat. Select Stop when the pump
stops. If the baseline is ramping or goes off scale when the pump is stopped, it
indicates air in the flow cell. Remove the air before proceeding to the next step.
7. Repeat Step 4 above using Buffer A (0% B).
Troubleshooting
8. Place the 5 non-barcoded microvial adapters in the sample rack. Fill 5 microvials
with Buffer A and place them in the microvial adapters. Place a stop tube after the
last adapter.
9. Select RUN/Worklist and start the run.
10. Select the Graph option. When the first sample trace starts to appear on the graph,
change the scale in order to place the trace in the middle of the graph. The scale
minimum should be 1 mV below the trace and the maximum 1 mV above the trace.
Select Set Scale on the screen to make the appropriate changes. Make the changes to
the scale permanent by selecting Yes when the screen appears. Failure to make these
changes causes the printed scaling to be off.
11. Save the Run Summary in order to obtain the injection numbers.
12. Export the raw data from the injections run to the floppy disc.
a. Place the floppy disc in the floppy drive (A:\).
b. Open Windows Explorer by right clicking on the Start button at the bottom
of the screen and selecting Explore.
c. Locate D:\CDMxx\RAWDATA.exe and start it by double clicking.
d. Enter the following information in the CDM Extract Raw Data screen
(Figure 8-15).
13. Select Extract RAW file(s). When the data is written to the floppy disc, a message
will appear indicating the process is complete. Select OK and then Exit on the
screen.
Troubleshooting
14. Remove the floppy disc from the VII computer and place it into the floppy drive of a
computer that has the Noise.xls file loaded.
15. Open the noise test spreadsheet and select Enable Macros. The file will be Read
Only.
16. Select the buffer system that was used (e.g. V2 A1c). Figure 8-16 shows the
spreadsheet screen.
25mV, either the jumper was not returned to its proper position on the board or the
detector was not zeroed prior to the test. Correct the problem and repeat the noise
test.
19. Continue analyzing the data by selecting Start Noise Test again. Select Yes to
continue and repeat Step 17 for the remaining injections. Look for consistency
between the traces. If they are not similar, or if the later tests fail, repeat the Noise
Test with fresh Buffer A. If these do not show improvement, additional repair of the
detector is required.
20. Once the noise test is complete, set the test back to the assay to be run. If GHb was
used, remove the old cartridge and replace it with a new cartridge.
Troubleshooting
8.8.1.1 Acceptable Noise Tests
Figures 8-17 through 8-19 show a variety of passed noise tests. Figure 8-19 shows
slight Fuzz (line not smooth) in the trace. This can be caused by a small bubble or
debris in the flow cell. Remove the bubble or debris and repeat the noise test.
The following messages will appear next to the tracing in the spreadsheet if a noise
test fails.
NOTE: Fuzz refers to a line that is unstable or lacks definition (not smooth).
Gradient Noise
Troubleshooting
Gradient Noise >100 mV? = wrong inj #.
Gradient Noise drops with each successive injection? = dirty dilution well or sample
line swaging issue.
Gradient Noise stable, but above limit? = try a new lamp or optic bench (in that
order), re-balance and re-test
Fuzz
Fuzz is almost always something in the flow cell, usually air. If it differs across the
trace, then there is also a pump issue (noisy 0 to 80 seconds = Pump A, 40 to 130
seconds = Pump B). Purge the flow cell and pump(s) as required and re-test.
Combo
Look for reproducible “3 hump” patterns. If trace changes markedly from injection
to injection, lamp is dying. Replace lamp, rebalance and re-test.
Drift
Refer to the Noise Test Flow Chart in Figure 8-21 for troubleshooting a failed noise
test. Once servicing is complete, repeat the Noise Test.
NOTE: An unstable lamp can adversely affect noise test results. Verify that the
lamp was powered on for the appropriate amount of time before running or
repeating a noise test. Refer to Section 8.5.1.3 Lamp Stabilization for details.
Figure 8-21:
Noise Test
Flow Chart
Troubleshooting
• Temperature at power up
• Age of the lamp
The chart in Figure 8-22 shows the differences in drift between the old lamp on a
Troubleshooting
chromatographic station and a newly installed lamp immediately, and after 2 days.
Based on this data, it is important to allow adequate time for thermal equilibration (up
to 40 minutes) from a cold start up before performing a noise test. New lamps may
require additional time.
NOTE: All other parameters (fuzz, gradient noise, combo) were stable on both
lamps from the beginning.
9 Spare Parts
9.1 TURBO Upgrade Parts
The following tables show the parts required to upgrade a VARIANT II to a VARIANT II
TURBO and also required reliability parts. All other parts in the VCS and VSS remain the
same as those listed in the VARIANT II Service Manual.
Spare Parts
Part
Number
2200247 D-10/VIIT, 2 grooves to
Piercing facilitate
Needle Piercing Needle venting
2702361 VSST
Column
Heater
Block
2702362 VSST
Cartridge
Holder
Turbo 2.0
requires
additional
parts
2702360 VSS
Conical
Dilution
Chamber
2702356 VSST Z
Sensor PCB
294-255-7
2702357 VSST Z-
Axis PCB
294-254-4
Spare Parts
2702359 VSST Z-Axis VSST Z-Axis
Cable (5 Pin) Cable 2, 5 Pin
NOTE: The
Vespel rotor
seal (7750-16)
will have VS
stamped into
the surface.
The PEEK
rotor seal will
have PK
stamped into
the surface.
NOTE: Order
2 valves for
each Variant
VSS Pinch Valve D - V3, V4, V7 II upgrade.
Spare Parts
2702353 VSS
Internal
Waste Tank
Baffle
Appendix A
Appendix A
Fluidic Diagrams
Table of Contents:
Bio-Rad P/N *
270-2136 Cartridge Holder
270-2136
270-2137
270-2180
270-2138
270-2138
270-2138
270-2138
270-2138
270-2138
270-2138
270-2138
270-2096
270-2096
270-2139
270-2139
270-2143
270-2142
270-2143
270-2139
270-2139
270-2139
**
270-2140
270-2140
270-2140
270-2141
270-2141
270-2141
270-2141
270-2363
** SL 1-4 270-2084
SL 5-8 270-2083
Pre-cut with fittings
Tubing List
Bio-Rad P/N* Part No: ID x OD x L Material (Remark)
270-2021** L1: 1 x 2 x 550 Teflon Tube
270-2023** L2: 1 x 3 x 60 Si-Tube (CKD/HYN-3-1)
270-2023** L3: 1 x 3 x 145 Si-Tube (CKD/HYN-3-1)
270-2023** L4: 1 x 3 x 45 Si-Tube (CKD/HYN-3-1)
270-2023** L5: 1 x 3 x 100 Si-Tube (CKD/HYN-3-1)
270-2023** L6,7: 1 x 3 x 20 Si-Tube (CKD/HYN-3-1)
270-2023** L8: 1 x 3 x 50 Si-Tube (CKD/HYN-3-1)
270-2024 L9: 2 x 4 x 400 Si-Tube
270-2022** L10: 0.5 x 1.58 x 700 Teflon Tube
270-2025 L11: 3 x 5 x 730 Si-Tube (CKD/HYN-5-3)
270-2025 L12: 3 x 5 x 730 Si-Tube (CKD/HYN-5-3)
270-2025 L13: 3 x 5 x 760 Si-Tube (CKD/HYN-5-3)
270-2025 L20: 3 x 5 x 40 Si-Tube
270-2025 L21: 3 x 5 x 450 Si-Tube (CKD/HYN-5-3)
270-2026 L22,23: 4 x 7 x 450 Si-Tube
270-2025 L24 -27: 3 x 5 x 30 Si-Tube
270-2023 L28,29: 1 x 3 x 70 Si-Tube (CKD/HYN-3-
1)
270-2023 L30: 1 x 3 x 110 Si-Tube (CKD/HYN-3-1)
270-2023 L31: 1 x 3 x 70 Si-Tube (CKD/HYN-3-1)
270-2025 L32 -34: 3 x 5 x 30 Si-Tube
270-2026 L35: 4 x 7 x 190 Si-Tube
270-2025 L36: 3 x 5 x 250 Si-Tube (CKD/HYN-5-3)
270-2026 L37: 4 x 7 x 80 Si-Tube
270-2026 L38,39: 4 x 7 x 360 Si-Tube
270-2026 L40: 4 x 7 x 130 Si-Tube
270-2075 L41: 5 x 8 x 30 Si-Tube
270-2027 L42: 5 x 8 x 2.5m Si-Tube
Note: L14, L15, L16, L17, L18 and L19 are NOT
P/N 270-2133
fittings
P/N 270-2204
270-2028
Tube Fitting Set
Appendix B
Appendix B
Mechanical Drawings
Table of Contents:
VCS
VSS
Guard Cartridge
Appendix B
Appendix C
Appendix C
Table of Contents:
VCS
VSS
P/N 270-2199
P/N 270-2089
P/N 270-2191
P/N 270-2011 P/N 270-2012
P/N 270-2126
(Includes Sensor) (Includes Sensor)
P/N 270-2168
P/N 270-2127
P/N 270-2125
P/N 270-2188
P/N 270-2123
P/N 270-2122
P/N 270-2093
P/N 270-2082
P/N 270-0127
P/N 270-2120
P/N 270-2092
P/N 270-2082
P/N 270-2085
P/N 270-0153
P/N 270-2188
P/N 270-2146
P/N 270-2187 P/N 270-0206 P/N * See Below
P/N 270-2207
P/N 270-2200
P/N 270-2060 P/N 270-2073
(V3 or V4)
P/N 270-2068
P/N 270-2010
P/N 270-2074
P/N 270-2235
P/N 270-2048
P/N 270-2198
P/N 270-2062
P/N 270-2050 P/N 270-2066
P/N 270-2067
(PCB 256)
P/N 270-2071
P/N 270-2073
P/N 270-2250
P/N 270-2202
P/N 270-2223
P/N 270-2037
P/N 270-2037
P/N 270-2356 P/N 270-2065
(applies to 255-1 P/N 270-2206
P/N 270-2203
P/N 270-2355
through 255-6)
P/N 270-2038
P/N 270-2247 (either)
P/N 270-2041
P/N 270-2194
P/N 270-2065
(applies to 255-1 P/N 270-2069 (for any) P/N 270-2234 P/N 270-2205
through 255-6)