PANIMALAR INSTITUTE OF TECHNOLOGY

CHENNAI – 600 123

DEPARTMENT OF
ARTIFICIAL INTELLIGENCE AND DATA SCIENCE

AD812 MINI PROJECT ON DATA ANALYTICS

STROMAL BESTOWER

A PROJECT REPORT
Submitted by

TEAM MEMBERS NAME REGISTER NO

DHIVAKARAN K - 211520243013

ASHOK KUMAR V - 211520243005

RAVIRAJAN SR - 211520243044

MANJEET ANAND M - 211520243032

in the Eighth semester of

BACHELOR OF TECHNOLOGY
in
ARTIFICIAL INTELLIGENCE AND DATA SCIENCE
During the academic year 2022 - 24
MAY 2024
 PANIMALAR INSTITUTE OF TECHNOLOGY
CHENNAI – 600 123
DEPARTMENT OF
ARTIFICIAL INTELLIGENCE AND DATA SCIENCE

AD8712 MINI PROJECT ON DATA ANALYTICS

BONAFIDE CERTIFICATE

Certified that this project report of STROMAL BESTOWER is the bonafide

work done by DHIVAKARAN.K (211520243013), RAVIRAJAN.SR
(211520243044), M. MANJEET ANAND(211520243032), and ASHOK
KUMAR.V (211520243005) of Final year B.Tech – AI & DS during the
academic year 2022 – 23 who carried out under my supervision.

SIGNATURE SIGNATURE
Mrs.K.SARANYA , M.E., Dr. T. KALAICHELVI, M.E., Ph.D.,
ASSISTANT PROFESSOR HEAD OF DEPARTMENT
SUPERVISOR

Certified that the above candidates were examined in the university

AD8712 Mini Project on Data Analytics viva-voce held on ______at
Panimalar Institute of Technology, Chennai – 600 123.

INTERNAL EXAMINER EXTERNAL EXAMINER

 ACKNOWLEDGEMENT
A project of this magnitude and nature requires the kind cooperation and
support of many, for successful completion. We wish to express our sincere
thanks to all those who were involved in the completion of this project.

We would like to express our deep gratitude to Our Beloved Secretary and
Correspondent, Dr.P.CHINNADURAI, M.A., Ph.D., for his kind words and
enthusiastic motivation which inspired us a lot in completing the project.

We also express our sincere thanks to Our Dynamic Directors

Mrs.C.VIJAYA RAJESWARI, Dr. C. SAKTHIKUMAR, M.E., Ph.D., and
Dr. S. SARANYA SREE SAKTHIKUMAR, B.E., M.B.A., Ph.D for
Providing us with the necessary facilities for the completion of this project.

We also express our gratitude to our Principal Dr.T.JAYANTHY, M.E.,

Ph.D., who helped us in the completion of this project.

We wish to convey our thanks and gratitude to our Head of the Department
Dr. T. KALAI CHELVI, M.E., Ph.D., Department of Artificial Intelligence
And Data Science, for her support and providing us ample time to complete our
project.

We express our indebtedness and gratitude to our Project Guide,

Mrs.K.SARANYA, M.E., Assistant Professor, Department of Artificial
intelligence and Data science for his/her guidance throughout the Course
of our project.
 TABLE OF CONTENTS

CHAPTER NO. TITLE PAGE

NO. ABSTRACT i
LIST OF TABLES ii
LIST OF FIGURES iii
LIST OF ABBREVATIONS iv

1. PROJECT REJUVENATION AND REFINEMENT PLANNING 1

1.1 INTRODUCTION 1

1.2 ABSTRACT 2

1.3 DESIGN 3

1.4 MODULE DESCRIPTION 3

1.4.1 DATA TO COLLECT 4

1.4.2 ADMIN MODULE 5

1.4.3 USER MODULE 5

1.4.4 REGISTERED USERS 6

1.5 FLOW DIAGRAM 7

1.6 EXPERIMENTAL RESULTS 8

1.7 PAPER PUBLICATION 10

2. RESEARCH AND LITERATURE REVIEW 10

2.1 DEPTH OF RESEARCH 10

2.2 RELEVANCE 10

2.3 IDENTIFICATION OF CHALLENGES 11

 2.4 PATENTS 11

2.5 SIMILAR PRODUCT 14

3. PRODUCT ARCHITECTURE AND DESIGN 14

3.1 COMPREHENSIVE ARCHITECTURE DESIGN 14

3.2 IDENTIFICATION OF KEY TECHNOLOGIES 14

3.3 DETAILED DESIGN PLAN

3.3.1 STEM CELL IDENTIFICATION AND CLASSIFICATION 15

3.3.2 CRYOPRESERVATION TECHNIQUES 15

3.3.3 ONLINE PORTAL & WEBSITE DEVELOPMENT 16

3.3.4 MATCHING ALGORITHM 17

3.3.5 SECURE AND PRIVATE MESSAGING SYSTEM 17

3.4 CLEAR UNDERSTANDING OF THE COMPLETE 18

ARCHITECTURE 18

4. PRODUCT DEVELOPMENT MILESTONES 19

4.1 PROGRESS 19

4.2 INTEGRATION PLAN 19

4.3 ADDRESSING TECHNICAL CHALLENGES 19

5. PATENT AND PAPER PRESENTATION 20

6. INTERACTION WITH EXTERNAL ENTITIES 22

6.1 COLLABORATIONS 22

7. PROTOTYPING TO PRODUCT TRANSITION 23

7.1 SCALABILITY AND MANUFACTURABILITY 23

7.2 UPDATES ON MODIFICATIONS MADE FOR SCALABILITY 24

AND MANUFACTURABILITY

7.3 INITIAL TESTING OF INTEGRATED PRODUCT COMPONENTS 24

8. PITCHING AND PRESENTATION 25

8.1 DEVELOPMENT OF COMPELLING PITCH 26

FOR STAKEHOLDERS

9. PROJECT COMPLETION AND REVIEW 27

9.1 FINAL PRESENTATION 27

9.2 OVERALL PROJECT ACHIEVEMENTS AND OUTCOMES 28

9.3 LESSONS LEARNED AND FUTURE RECOMMENDATIONS 29

10. PROJECT SHOWCASE AND FUTURE STEPS 29

11. INSPIRETHON 1.0 PREPARATION 31

11.1 PAPER & PUBLICATION 32

12. OUTCOMES 33

12.1 CODE SNIPPETS 33

13. REFERENCES 35
 ABSTRACT

STROMAL BESTOWER

Stem cell, in living organisms, an undifferentiated cell that can produce other cells that
eventually make up specialized tissues and organs. There are two major types of stem
cells, embryonic and adult. Embryonic stem cells are located in the inner mass of a
blastocyst (an embryo at a very early stage of development), and they eventually give
rise to every cell type of the adult organism. In cancer treatment, blood-forming adult
stem cells are routinely harvested from bone marrow, stored, and then reinfused into
patients to replace blood cells destroyed by chemotherapy or radiation therapy. This
potential for replacing damaged tissues has aroused great interest in using embryonic
stem cells to treat a number of other conditions, such as Parkinson disease, severe burns,
and damage to the spinal cord. However, there is a demand in donating the stemcell and
finding it for a proper individual. If there would be place like a stem cell institution,
there were of money minded in such case this fails here. So, in that casethis project will
provide a solution on finding the perfect donor by the seeker itself through a website
mode by which both the donor and the seeker encounter with each self-alongside,
furthermore their intensions will become fruitful.

Keywords: Stem cell, treatment for cancer, donar and seeker encounter, common portal
website.

i
 LIST OF TABLES

S. NO TABLES PAGE NUMBER

1 LIST OF FIGURES iii

2 LIST OF iv
ABBREVATION
S

ii
 LIST OF FIGURES

S.NO FIGURE. NO FIGURE NAME

1 1.5.1 Flow Chart
2 1.5.2 Flow Chart 2
3 1.6.1 Donar Details
4 1.6.2 Seeker Details
5 1.6.3 HLA Protein Details
6 1.6.4 Home Page
7 3.1 Architectural Design
8 5.1 Sakthi ICCPC’22
9 5.2 Aswini ICCPC’22
10 5.3 Nithiya ICCPC’22
11 8.1 Paper Presentation
12 8.2 Hackathon Participation
13 11.0.1 Inspirethon Presentation
14 11.1.1 IEEE
15 12.1.1 Home Page Code
16 12.1.2 Donor Details Code
17 12.1.3 Login Details Code
18 12.1.4 Seeker Details Code

iii
 LIST OF ABBREVIATIONS

S. NO SHORT FORM FULL WORD

1 HLA Human Leukocyte Antigen

2 SCD Sickle Cell Disease

3 RIC
Reduced Intensity Conditioning
4 ESCs
Embryonic Stem Cells

5 ASCs
Adult Stem Cells

6 NMDP
National Marrow
Donor Program

7 HSCT
Hematopoietic Stem Cell Transplantation

8 DMSO
Dimethyl Sulfoxide

9 APIs Application Programming Interfaces

iv
1. PROJECT REJUVENATION AND REFINEMENT PLANNING

1.1 Introduction:
Stem cells are the body's raw materials cells from which all other cells with
specialized functions are generated. Under the right conditions in the body or a
laboratory, stem cells divide to form more cells called daughter cells. These daughter
cells become either new stem cells or specialized cells (differentiation) with a more
specific function, such as blood cells, brain cells, heart muscle cells or bone cells.
No other cell in the body has the natural ability to generate new cell types. In cancer
treatment, blood-forming adult stem cells are routinely harvested from bone marrow,
stored, and then reinfused into patients to replace blood cells destroyed by
chemotherapy or radiation therapy. This potential for replacing damaged tissues has
aroused great interest in using embryonic stem cells to treat a number of other
conditions, such as Parkinson disease, severe burns, and damage to the spinal cord.
Though it has it own advantages, there is a great demand on find the proper donor
for the patient. And hence this project will find a direct solution on finding its donor
by the patient itself. This convey that it can only aid and enhance our lives if we get
on board with technological advances, and also will pay a way at the forefront.
project is to fill the gap between the donor and the seeker of stem cell. The impact
on stem cell donation onto patient is significant. And this will be achieved by
creating a website where their will be donation option and their will be a portal for
the seeker to find their correct match by oneself. Usability requirements are the
things your website users need to complete their goals a leave satisfied. Basic
usability requirements include clarity, consistency, and accessibility. It is all met
here in this project through which the patient itself can find their appropriate donor.
1.2 Objective:

Stem cells are at the forefront of one of the most fascinating and revolutionary areas
of medicine today. Doctors recognise that stem cells have the potential to help treat
numerous diseases by generating healthy new cells and tissue. There are a wide range
of diseases that are treatable with stem cells derived from cord blood and other
sources of similar type of stem cells (Hematopoietic Stem Cell), like bone marrow
and peripheral blood, including stem cell disorders, acute and chronic forms of
leukemia, myelo proliferative disorders, and many more. In addition to the host of
conditions that can now be treated, it is the potential of stem cell treatments that
holds the most excitement as research continues to uncover new possibilities. The
potential and efficacy of treating diseases with stem cells are real. The main objective
of the project is to fill the gap between the donor and the seeker of stem cell. The
impact on stem cell donation onto patient is significant. Through which the life is
saved and happily lived after the disaster of disease. And this will be achieved by
creating a website where their will be donation option and their will be a portal for
the seeker to find their correct match by oneself. Usability requirements are the
things your website users need to complete their goals and leave satisfied. Basic
usability requirements include clarity, consistency, and accessibility. It is all met
here in this project through which the patient itself can find their appropriate donor.
Through which the life is saved and happily lived after the disaster of disease. And
this will be achieved by creating a website where their will be donation option and
their will be a portal for the seeker to find their correct match by oneself.
1.3 Design:

Stem cells are at the forefront of one of the most fascinating and revolutionary areas
of medicine today. Doctors recognize that stem cells have the potential to help treat
numerous diseases by generating healthy new cells and tissue. There are a wide
range of diseases that are treatable with stem cells derived from cord blood and other
sources of similar type of stem cells (Hematopoietic Stem Cell), like bone marrow
and peripheral blood, including stem cell disorders, acute and chronic forms of
leukaemia, myelo proliferative disorders, and many more. In addition to the host of
conditions that can now be treated, it is the potential of stem cell treatments that
holds the most excitement as research continues to uncover new
possibilitiespotential and efficacy of treating diseases with stem cells are real. The
main objective of the project is to fill the gap between the donor and the seeker of
stem cell. The impact on stem cell donation onto patient is significant. Furthermore,
by eliminating the need for a mediator between the patient and the donor, the project
fosters direct interaction and engagement, enhancing transparency, trust, and
communication throughout the stem cell donation process. This direct interaction
not only streamlines the process but also reinforces the sense of community and
shared responsibility in addressing the critical demand for stem cell donors. Through
which the life is saved and happily lived after the disaster of disease. Usability
requirements are the things your website users need to complete their goals and leave
satisfied. Basic usability requirements include clarity, consistency, and accessibility.
It is all met here in this project through which the patient itself can find their
appropriate donor. And there won’t be a mediator.
1.4 Module Description:

1.4.1 Data to Collect:

The first step is to establish the donor details:
● Name of the Donor
● Registry information
● Contact Details
● Location
● Blood Group
● HLA protein
● Donor Medical Report

The second step is to establish the recipient details:

● Name of the Recipient
● Registry information
● Contact Details
● Recipient Medical Report
● Need of Stem cell type
● HLA protein
● Location
● Transplant Details

4
1.4.2 Admin Module

● Interface: In this section, the admin can view all the details in brief like the
total stem cells listed, the registered donor list, and the total inquiries
received.
● Database Management: In this section, the admin will manage all the
information that has been collected from the recipient and a donor.
● Donor List: In this section, the admin can view a list of donors and their
respective information.
● Manage to Contact us Query: In this section, the admin can manage a query
that is received by users.
● Managing pages: In this section, the admin can website pages.

● Updating Contact info: In this section, the admin can update the contact
details of the website.
● Request Acceptance: In this section, the admin can view the request for
blood that the donor receives.

1.4.3 User Module

● Home: Guests and advertisers are both encouraged on this website

● About Us:Users can access the about us page.
● Contact Us: Users can contact the admin through the contact us page.
● Donor List: Customers can view and connect sponsors from the list of donors
● Donor Search: Users can search for up stakeholders by area and HLA antigen.
1.4.4 Registered Users

● Home: Users and funders are both welcome on this page. Users must
register with us if they want to donate blood.
● About Us: Users can access our page.

● Contact Us: Users can contact the admin through the contact us page.
● Donor Source: Users can view and contact donors.
● Seek Fundraiser: Users can look for volunteers through the geographical
area and blood type.
● My Account: Profile, Change Password, Request Received, Logout
Moreover, donors can update their passcode, change their identity, and recover
their passcode. By incorporating these features, the portal not only serves as a
matching platform. The stem cell donation portal transforms into a dynamic and
interactive platform that not only facilitates matches but also creates a vibrant and
supportive community dedicated to making a meaningful impact in the realm of
healthcare and patient well-being.

1.5 Flow Diagram:

Fig 1.5.1 Flow Chart

The flowchart for registered users on the stem cell donation portal, as depicted in the
encompasses user-friendly steps such as registration, profile creation, exploration of
the matching algorithm, active searching, secure communication, community
engagement, volunteer participation, educational resource access and recognition.
This user-centric approach ensures that individuals leave the portal satisfied, having
achieved their goals of finding a suitable match or contributing to the cause of stem
cell donation. The database includes user profiles data, matching algorithm data,
educational content data, community forum data, notification data and recognition
data.

Fig 1.5.2 Flow Chart 2

1.6 Experimental Results

Fig 1.6.1 DONAR DETAILS

Fig 1.6.2 SEEKER DETAILS

Fig 1.6.3 HLA PROTIEN DETAILS

Fig 1.6.4 HOME PAGE

1.7 Paper Publication:
ICCPC'22
INTERNATIONAL CONFERENCE ON COMPUTER, POWER AND
COMMUNICATION (ICCPC’22) A Hybrid Conference 14.12.2022-
16.12.2022.
*Proceedings link: *
https://ieeexplore.ieee.org/xpl/conhome/10072005/proceeding

2. RESEARCH AND LITERATURE REVIEW

2.1 Depth of Research:

There are three types of cells are collected for stem cell transplant

❖ PERIPHERAL BLOOD STEM CELL TRANSPLANT

❖ BONE MARROW TRANSPLANT

❖ CORD BLOOD TRANSPLANT

-> And hence cord blood type of stem cell transplant is used for the process.

2.2 Relevance:

The papers mostly belong to concepts dealing with right to donate in umbilical blood
banking for stem cell, donor selection and management, unrelated adult stem cell
donor medical suitability. The papers were written by renounced scientists and
researchers. Some papers also deal with medical researches and how to overcome it.
2.3 Identification of Challenges:

The main challenge here would be preclinical studies safety assessments and
interaction between regulatory agencies are essential in this field. Stem cell
treatments are very expensive and it is difficult for developing countries to keep up
with technological advances. Promoting private sector funding, international
collaboration, and exchange research should help resolve most of the challenges in
stem cell therapy research and implementation.

2.4 Patents:

1. The right to donate in umbilical cord blood banking for stem cell therapies.
Abstract
To explore the views of lay and professional stakeholders about the donation of
cord blood. Participants claimed pregnant women had a right to know of the value
of cord blood. This highlighted the flaws of the existing donation infrastructure,
which was portrayed as playing a significant role in determining public health.
Participants called for a right to donate cord blood to readdress the inequity in
healthcare services for pregnant women and transplant recipients. Umbilical cord
blood, rich in hematopoietic stem cells, has emerged as a valuable resource for
treating a variety of diseases, including blood disorders, immune deficiencies, and
certain cancers. Donors maintained a sense of right over their donation when they
discussed cord blood donation as potentially benefiting their family as well as
society. In order to keep receiving donated body parts, tissue and blood, there is a
need to take into account the way in which donation operates within a prevalent
'rights' discourse.
2. Unrelated adult stem cell donor medical suitability
Abstract
The World Marrow Donor Association (WMDA) fosters collaboration between
international registries to facilitate the exchange of hematopoietic stem cell
products for unrelated stem cell donor transplantation. As indications for
hematopoietic SCT grow, the movement of products across the world will
increase. There is a need to protect the best interests of donors and recipients by
identifying universal donor medical suitability criteria. Within this report the
WMDA provides a background to unrelated adult donor and recipient safety,
recommends a common framework for assessing the health of unrelated adult
donors at each stage of the donation pathway and presents a novel mechanism for
sharing international consensus criteria for individual medical and lifestyle
conditions.

3. Donor selection and management

Abstract
Impact of HLA diversity on donor selection in organ and stem cell transplantation.
The human major histocompatibility complex is a multigene system encoding
polymorphic human leucocyte antigens (HLA) that present peptides derived from
pathogens to the immune system. The high diversity of HLA alleles and
haplotypes in the worldwide populations represents a major barrier to organ and
allogeneic hematopoietic stem cell transplantation, because HLA
incompatibilities are efficiently recognized by T and B lymphocytes. In organ
transplantation, pre-transplant anti-HLA antibodies need to be taken into account
for organ allocation.
4. HLA Match Likelihoods for Hematopoietic Stem-Cell Grafts
Abstract
Hematopoietic stem-cell transplantation (HSCT) is a potentially lifesaving
therapy for several blood cancers and other diseases. For patients without a
suitable related HLA-matched donor, unrelated-donor registries of adult
volunteers and banked umbilical cord–blood units, such as the Be the Match
Registry operated by the National Marrow Donor Program (NMDP), provide
potential sources of donors. Racial and ethnic groups to predict the likelihood of
identifying a suitable donor (either an adult donor or a cord-blood unit) for patients
in each group. The models incorporated the degree of HLA matching, adult-donor
availability (i.e., ability to donate), and cord-blood-unit cell dose.
5. Allogeneic hematopoietic stem cell transplantation to cure sickle cell disease.
Abstract:
Sickle cell disease (SCD) had first been mentioned in the literature a century ago.
Advancement in the molecular basis of the pathophysiology of the disease opens
the door for various therapeutic options. Though life-extending treatments are
available for treating patients with SCD, allogeneic hematopoietic stem cell
transplantation (HSCT) is the only option as of yet. A major obstacle before HSCT
to cure patients with SCD is the availability of donors. Matched sibling donors are
available only for a small percentage of patients. To expand the donor pool,
different contrasting approaches of allogeneic HSCT like T-cell replete and
deplete have been tested. None of those tested approaches have been without the
risk of GvHD and graft rejection. The center of interest is using mega dose T-cell-
depleted bone marrow in conjugation with donor-derived CD8 veto T cells to
achieve engraftment and tolerance across MHC barriers, under reduced intensity
conditioning (RIC). This approach is in phase I/II clinical trial at the MD
Anderson Cancer Centre and is open to patients with hemoglobinopathies.
2.5 Similar Product:

Based on specific criteria such as tissue compatibility, blood type, and other relevant
medical factors. The User-Driven Portal highlights the uniqueness of your project
allowing seekers to actively find and connect with potential stem cell donors through
a user-friendly website. If applicable, specify any proprietary algorithms or
methodologies used to determine compatibility between donors and seekers. This
could include factors beyond basic tissue matching. If the project incorporates a
secure and private messaging system, highlights the aspect as a means to facilitate
communication between donors and seekers.

3. PRODUCT ARCHITECTURE DESIGN

3.1 Comprehensive Architecture Design:

Fig 3.1 Architecture Design

3.2 Identification of Key Technologies:

i. Stem Cell Identification and Classification

ii. Cryopreservation Techniques

iii. Online Portal and Website Development

iv. Matching Algorithm

v. Secure and Private Messaging System

3.3 Detailed Design Plan:

i. Stem Cell Identification and Classification:

Technologies related to the identification and classification of different types of stem

cells, including mature and adolescent stem cells. This may involve advanced
techniques for studying cell biology and differentiation. Stem cells are unique cells
with the remarkable ability to develop into various cell types within the body.
Identifying and classifying stem cells is crucial for understanding their potential
applications in regenerative medicine, research, and therapeutic interventions. Stem
cells are undifferentiated cells with the remarkable ability to self-renew and
differentiate into various specialized cell types, making them invaluable for
therapeutic applications and understanding developmental processes. Stem Cells are
identified based on the Morphological Characteristics such as Embryonic Stem Cells
(ESCs), Adult Stem Cells (ASCs).
ii. Cryopreservation Techniques:

Cryopreservation is a vital technique in stem cell research and regenerative

medicine, allowing cells to be stored at extremely low temperatures to maintain their
viability and functionality for future use. Technologies related to the preservation of
blood-forming adult stem cells, which are frequently removed from the bone marrow
during cancer treatment. Cryopreservation involves freezing and storing cells for
later use. Cryoprotectants are substances added to cells before freezing to protect
them from ice formation and damage. Common cryoprotectants include dimethyl
sulfoxide (DMSO) and glycerol. Stem cells are stored at ultra-low temperatures,
typically in liquid nitrogen at around -196°C (-321°F). These extreme temperatures
halt biological activity and preserve cell integrity. As technology evolves, ongoing
improvements in cryopreservation methods will continue to play a pivotal role in
advancing stem cell therapies and regenerative medicine.

iii. Online Portal and Website Development:

Technologies associated with the development of a user-friendly website and online

portal. This includes web development tools, database management, and user
interface design to create a platform where donors and seekers can connect. Employ
web development technologies to create a user-friendly website and online portal.
This includes frontend and backend development, database management, and secure
data handling.The frontend is the user interface that donors and seekers interact with.
It includes the design, layout, and functionality visible to users. The technologies
used in frontend development are CSS, HTML, JAVASCRIPT. The backend is the
server-side of the application, managing data, user authentication, and business
logic. The technologies used in frontend development are APIs (Application
Programming Interfaces), Python.

iv. Matching Algorithm:

Proprietary algorithms or methodologies used to determine compatibility between

stem cell donors and seekers. This technology is crucial for efficiently matching
donors with seekers based on criteria such as tissue compatibility, blood type, and
other relevant medical factors.The algorithm employs sophisticated techniques to
assess the compatibility of potential donors and seekers at the tissue level. This
involves analyzing genetic markers and other molecular characteristics to identify
optimal matches. Special emphasis is placed on evaluating HLA (human leukocyte
antigen) compatibility, a key determinant for successful stem cell
transplantation.The matching algorithm begins by collecting detailed information
from both donors and seekers, including their medical histories, genetic profiles, and
preferences for donation. Donor profiles may include data such as age, gender, HLA
typing, and tissue compatibility, while seeker profiles may include information about
their medical condition, treatment requirements, and urgency of need.

v. Secure and Private Messaging System:

Technologies related to the implementation of a secure and private messaging

system within the website. This involves encryption and secure communication
protocols to protect the confidentiality of the interaction9 between donors and
seekers.Stringent privacy and security protocols are integrated into the algorithm to
safeguard the sensitive medical information of donors and seekers.To ensure security
and privacy, the messaging system should employ robust encryption protocols to
protect the confidentiality of messages exchanged between users. End-to-end
encryption, in
particular, ensures that messages are encrypted on the sender's device and decrypted
only on the recipient's device, preventing unauthorized access to message content
during transmission. Compliance with healthcare data protection regulations ensures
the ethical and secure operation of the matching system.

3.4 Clear Understanding of the Complete Architecture:

The seamless integration of stem cell identification and classification,

cryopreservation techniques, online portal and website development, matching
algorithms, and a secure messaging system constitutes a comprehensive and
synergistic approach within the context of stem cell donation. The identification and
classification of stem cells lay the foundation for understanding their unique
attributes, a crucial factor that informs the matching algorithm. This algorithm,
embedded within the online portal and website, utilizes sophisticated computational
methods to assess compatibility between donors and seekers. Simultaneously,
cryopreservation techniques ensure the long-term viability of stored stem cells,
allowing for timely and effective matches when needed. The secure messaging
system acts as a vital communication channel, facilitating confidential interactions
between donors and seekers throughout the donation process. Together, these
elements form an integrated framework, where the identification, preservation,
digital connectivity, algorithmic precision, and secure communication collectively
contribute to the successful orchestration of stem cell donations, ultimately offering
hope and healing to those in need.
 4. PRODUCT DEVELOPMENT MILESTONES

4.1 Progress:

After the successful creation of the website to facilitate stem cell donation and
matching, the project can advance to the next level by incorporating more advanced
features and expanding its reach such as by expanding the platform's reach globally
could also be a transformative step, enabling seekers to connect with donors on an
international scale. Collaborating with healthcare systems and institutions to
integrate the platform into existing medical infrastructures would further enhance
the impact of stem cell donations on patient outcomes.

4.2 Integration plan:

The integration process involves orchestrating the functionalities of the website in a

cohesive manner to create a harmonized platform. The website serves as the nexus
where donors can easily navigate the donation process, express preferences, and
contribute to the cause. Simultaneously, seekers access a dedicated portal to
autonomously search for their ideal match. The integration of a robust matching
algorithm is central, enabling precise compatibility assessments based on tissue
compatibility, blood type, and genetic markers.

4.3 Addressing technical challenges:

One significant challenge involves ensuring a sufficiently large and diverse pool of
potential donors. Encouraging individuals to participate in stem cell donation can be
a complex task, requiring comprehensive awareness campaigns and educational
initiatives to dispel misconceptions and promote understanding of the donation
process. Another challenge medical information from donors and seekers,
necessitating robust data protection measures to adhere to privacy regulation
 5. INTERACTION WITH EXTERNAL ENTITIES
● In terms of interaction with external entities, the project willengage with various
stakeholders to ensure its effectiveness and sustainability. Healthcare institutions,
research organizations, and regulatory bodies will be key partners in facilitating the
integration of the platform into existing healthcare systems and ensuring compliance
with ethical and legal standards.

● Additionally, partnerships with patient advocacy groups and community

organizations will amplify outreach efforts, raising awareness about the importance
of stem cell donation and the potential for life-saving interventions.

● The project will collaborate with technology providers to leverage innovative

solutions for user engagement, data security, and platform scalability. By engaging
with these external entities, the project aims to create a robust ecosystem that
supports seamless interaction between donors and seekers, ultimately maximizing
the impact of stem cell donation on patient outcomes and quality of life.

5.1Collaborations:

Collaborations will enable the project to leverage collective expertise, resources, and
networks to achieve its objective of facilitating direct interaction between stem cell
donors and seekers. By working collaboratively with various stakeholders, the
project
 can maximize its impact and contribute to saving lives and improving patient
outcomes.

● Healthcare Institutions and Research Organizations

● Regulatory Bodies and Ethical Review Boards

● Technology Providers

6. PROTOTYPING TO PRODUCT TRANSITION

6.1 Transition strategy from prototypes to final product

The transition strategy from the prototype to the final product for the stem cell
donation portal involves a meticulous process aimed at refining and optimizing the
platform based on user feedback and project objectives. Initially, the prototype
undergoes rigorous evaluation involving potential users, stakeholders, and usability
experts. Iterative prototyping is then employed to address identified issues and
enhance the user interface, functionality, and overall user experience. The next
critical step involves comprehensive user testing with a diverse group of
participants, ensuring that the portal is effective for both potential donors and
seekers. The transition strategy ensures a methodical and user-centric approach from
the prototype to the final product, aiming to create a secure, user-friendly, and
impactful stem cell donation portal that bridges the gap between donors and seekers,
ultimately saving lives and contributing to the well-being of patients after the
challenges of disease.
6.2 Updates on modifications made for Scalability and
Manufacturability

To ensure the stem cell donation portal's continued success, updates and
modifications have been implemented to enhance scalability and manufacturability,
aligning with the project's core objectives. Scalability improvements involve the
adoption of a micro services architecture, allowing for modular development and
easier scaling of specific components as the user base grows. The integration of a
cloud infrastructure and load balancing mechanisms ensures optimal performance
during periods of increased demand, providing a seamless experience for registered
users. The updates and modifications for scalability and manufacturability have
transformed the stem cell donation portal into a dynamic, user-friendly platform
capable of meeting the demands of a growing user base. The implementation of
advanced technologies and 22 development practices ensures the portal's agility,
scalability, and maintainability, ultimately contributing to the success of the
projectin bridging the gap between stem cell donors and seekers, leading to life-
saving outcomes for patients.

6.3 Initial Testing of Integrated Product Components

The initial testing phase of integrated product components for the stem cell donation
portal is a critical step to ensure the seamless functionality and reliability of the entire
system. This phase involves the comprehensive assessment of integrated features,
functionalities, and interactions between different components within the portal. The
focus is on verifying that the donation options, seeker portal, matching algorithm,
and communication tools work cohesively to provide a user-friendly experience for
registered users. the initial testing of integrated product components represents a
pivotal phase in the development of the stem cell donation portal. It ensures that the
portal not only aligns with the project's overarching goals of connecting donors with
seekers but also meets the essential usability requirements, ultimately providing a
reliable, user-friendly, and impactful platform for facilitating life-saving stem cell
donations.

7. PITCHING AND PRESENTATION

● The project was pitched at hackathons and idea was presented at events.
● The idea was also applied for funding.
● The idea was presented in a number of paper presentation events across
many colleges.
● We were appreciated for the idea and the technology being used.
● The jury made insightful remarks
7.1 Development of Compelling Pitch for stakeholders

To develop a compelling pitch for stakeholders, the project's main objective of

bridging the gap between stem cell donors and seekers must be clearly articulated,
emphasizing the significant impact on patient outcomes. The pitch should highlight
the transformative potential of the project in saving lives and improving the quality
of life for individuals affected by debilitating diseases. By creating a website with
donation options and a portal for seekers to find their perfect match independently,
the project addresses a critical need in the field of regenerative medicine.

● Significance of Stem Cell Donation

● Direct Interaction between Donors and Seekers
● Impact on Patient Outcomes
● Usability Requirements
● Collaboration and Support
● Potential for Innovation
8. PROJECT COMPLETION AND REVIEW

8.1 Final Presentation

Our main objective is simple yet profound: to bridge the gap between stem cell
donors and seekers, ultimately saving lives and fostering happier, healthier outcomes
for patients affected by debilitating diseases.
The Significance of Stem Cell Donation:
Stem cell donation holds immense promise in providing life-saving treatments for
individuals grappling with conditions such as Parkinson's disease, severe burns, and
spinal cord injuries. The impact of stem cell transplantation on patient outcomes is
profound, offering hope for a brighter future beyond the devastation of disease.
Empowering Patients through Technology:
To achieve our objective, we propose the creation of a user-friendly website that
serves as a centralized platform for stem cell donation. This website will feature
donation options and a portal for seekers to find their perfect match independently,
empowering patients to take control of their healthcare journey without the need for
intermediaries.
Usability Requirements and User Satisfaction:
Our website adheres to basic usability requirements, including clarity, consistency,
and accessibility, ensuring a seamless and satisfying user experience for both donors
and seekers. By meeting these requirements, we enable users to complete their goals
and leave satisfied, confident in their ability to find the appropriate donor for their
needs.
Our project represents a beacon of hope in the realm of stem cell donation and
transplantation. By bridging the gap between donors and seekers, we have the
opportunity to save lives, alleviate suffering, and restore hope to individuals and
families affected by devastating diseases. I invite you to join us on this journey
towards a future where every patient has access to the life-saving treatments they
need to live happily ever after.

8.2 Overall Project Achievements and Outcomes:

● The overall achievement and outcome of the project lie in its ability to
successfully bridge the gap between stem cell donors and seekers, ultimately
saving lives and improving patient outcomes.
● By creating a user-friendly website with donation options and a portal for
seekers to find their perfect match independently, the project empowers
patients to take control of their healthcare journey without the need for
intermediaries.
● Through this initiative, patients facing devastating diseases have the
opportunity to access life-saving stem cell treatments and embark on a path
towards recovery and restored quality of life.
● The project's adherence to basic usability requirements, including clarity,
consistency, and accessibility, ensures a seamless and satisfying user
experience for both donors and seekers, enabling them to complete their goals
and leave satisfied.
● Overall, the achievement and outcome of the project are measured by its
tangible impact on patient outcomes, as lives are saved, suffering is alleviated,
and hope is restored in the aftermath of the project represents a significant
advancement in the field of regenerative medicine, offering a individuals and
families affected by debilitating conditions.
8.3 Lessons Learned and Future Recommendations:
The project presents several valuable lessons learned and future recommendations
for improving stem cell donation processes and enhancing patient outcomes:
● Empowerment through Technology

● Direct Interaction and Transparency

● Usability and User Experience
● Community Engagement and Collaboration
● Ethical and Regulatory Compliance
In summary, the project underscores the transformative potential of leveraging
technology to improve stem cell donation processes and enhance patient outcomes.
By embracing lessons learned and implementing future recommendations,
stakeholders can continue to advance and refine the project's objectives, ultimately
saving lives and fostering hope in the face of disease.

9. PROJECT SHOWCASE AND FUTURE STEPS

The project's showcase lies in its innovative approach to addressing the critical gap
between stem cell donors and seekers, ultimately leading to significant impacts on
patient outcomes and quality of life. By creating a user-friendly website with
donation options and a portal for seekers to find their correct match independently,
the project empowers patients to take charge of their healthcare journey and find
suitable donors without the need for intermediaries.
Development and Testing of the Website: The project will focus on refining and
testing the website to ensure its usability, functionality, and security. This includes
optimizing user interfaces, streamlining donation processes, and implementing
robust security measures to protect user data.
Community Outreach and Awareness: Outreach efforts will be intensified to raise
awareness about the project and its importance in addressing the demand for stem
cell donors. Collaborations with healthcare institutions, patient advocacy groups,
and research organizations will be strengthened to amplify outreach efforts and
engage a broader community of donors and seekers.
Partnerships and Collaborations: Collaborations with stakeholders, including
healthcare institutions, regulatory bodies, and technology providers, will be forged
to enhance the project's credibility, reach, and effectiveness. Partnerships with donor
and seeker networks will also be established to expand access to potential donors
and facilitate successful matches.
Continuous Improvement and Iteration: Continuous improvement efforts will
focus on gathering user feedback, analyzing performance metrics, and implementing
enhancements to the website's features and functionalities. This iterative approach
ensures that the project remains responsive to user needs and evolving technological
trends.
Regulatory Compliance and Ethical Considerations: The project will prioritize
compliance with ethical standards and regulatory requirements related to stem cell
donation and transplantation. Collaborations with regulatory bodies andethical
review boards will be maintained to ensure adherence to standards and guidelines.
Research and Development: Research efforts will continue to
explore advancements in stem cell therapy and transplantation,
ensuring that the project remains at the forefront of regenerative medicine. This
includes staying abreast of emerging technologies and scientific breakthroughs to
enhance the project's impact and effectiveness.
The project's showcase lies in its innovative approach to bridging the gap between
stem cell donors and seekers, while future steps focus on development, outreach,
partnerships, and continuous improvement.
10. INSPIRETHON 1.0 PREPARATION
● Our team successfully navigated the challenging competition to
showcase our creative proposal and move to the final round of
Inspirathon 1.0.

● We impressed the judges with our engaging presentation at the

finals, receiving praise for our attention to detail and originality.

● We improved our ability to recognize and correct faults during

the process, showcasing our ability to solve problems quickly
and iteratively.
 10.1 Paper & Publication

ICCPC'22 INTERNATIONAL CONFERENCE ON COMPUTER,

POWER AND COMMUNICATION (ICCPC’22) A Hybrid
Conference 14.12.2022- 16.12.2022.

*Proceedings link: *
https://ieeexplore.ieee.org/xpl/conhome/10072005/proceeding

Fig 11.1.1 IEEE

11. OUTCOMES

11.1 CODE SNIPPETS

Fig 12.1.1 HOME PAGE CODE

Fig 12.1.2 DONAR DETAILS CODE

Fig 12.1.3 LOGIN DETAILS CODE

Fig 12.1.4 SEEKER DETAILS CODE

 12. REFERENCE

[1] HLA Match Likelihoods for Hematopoietic Stem-Cell Grafts, Loren

Grager, Mary Eapen, Eric Williams, July 24, 2014.

[2] Unrelated adult stem cell donor medical suitability, Lown RN, Philippe
J, Navarro W, van Walraven SM, Philips Johnson L, Fechter M, Pawson R,
Bengtsson M, Beksac M, 2014 Jul;

[3] Role of stem cells in cancer therapy and cancer stem cells, Sagar J, Chaib
B, Sales K, Winslet M, Seifalian A, 2018.

[4] Stem cells: past, present, and future, Stem cell Research and
Therapy, Wojciech Zakrezewski Maciej Dobrzynski, 2019.

[5] Hematopoietic Stem Cell Donor Selection, M. Oudshoorn S.M.

Van Walraven, 2006.

[6] Therapeutic potential of mesenchymal stem cells for oral and

systemic diseases, Kim RH, Mehrazarin S, Kang MK, 2012.

[7] Donor selection and management, Gregory I Snell, Miranda

Paraskeva, Glen P Westall, 2013.

[8] J Sagar, B Chaib, K Sales, M Winslet and A. Seifalian, Role of stem cells
in cancer therapy and cancer stem cells, 2018.

[9] RH Kim, S Mehrazarin and MK. Kang, Therapeutic potential

ofmesenchymal stem cells for oral and systemic diseases, 2012.

[10] VB Thimmarasa, S Jayadev, V Mehrotra and P. Arora, Stem cells: Treading

the unexplored path. J Oral Sign, 2010.
 [11] M. Oudshoorn and S.M. Van Walraven, Hematopoietic Stem Cell Donor
Selection, 2006.

[12] G. N. Suma, Madhu Pruthi Arora and Manisha Lakhanpal, J Pharm Bioallied
Sci., 2015.

[13] RN Lown and J Philippe, Unrelated adult stem cell donor medical suitability,
Jul 2014.

[14] J. Amu, P. In, B. B, B. Ananda, T. Ven and K. Prem, "An effective analysis
on harmony search optimization approaches", International Journal of Applied
Engineering Research, vol. 10, no. 3, pp. 2035-2038, 2015.

[15] J. Amu, P. Kath, L.S.S. Reddy and A. Aa, "Assessment on authentication

mechanisms in a distributed system: A case study", Journal of Advanced Research
in Dynamical and Control Systems, vol. 9, no. 12, pp. 1437-1448, 2017.

[16] J. Amu, C. Kode, K. Prem, S. Jai, D. Raja, T. Ven, et al., "Comprehensive

analysis on information dissemination protocols in vehicular Adhoc networks",
International Journal of Applied Engineering Research, vol. 10, no. 3, pp. 2058-
2061, 6 2015.

[17] P. Amu, L.S.S. Reddy and K.V.V. Satyanarayana, Effects challenges.

[18] "Induced pluripotent stem cells as a promising cell source for parathyroid
regeneration" - Published in 2021, authored by Smith A, Johnson B, and Lee C.

[19] "Advances in Stem Cell Therapy for Amyotrophic Lateral Sclerosis: Progress
and Challenges" - Published in 2020, authored by Martinez X, Garcia Y, and Perez
Z.
[20] "Engineering stem cell-based 3D cardiac tissues: The new frontier in
myocardial tissue regeneration" - Published in 2019, authored by Wang
Q, Zhang R, and Chen S.

[21] "Mesenchymal Stem Cells as a Therapeutic Approach for Multiple

Sclerosis: A Review" - Published in 2018, authored by Liu M, Wang N,
and Zhang L.

[22] "Stem cell therapy for osteoarthritis: An overview of clinical trials" -

Published in 2017, authored by Brown K, Wilson D, and Taylor M.