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1E_The Cell
1E_The Cell
(i) Sectioning- refers to cutting/ making of thin sections to allow light to pass through.
It is done by use of a sharp razor blade to ensure that cells are not distorted.
(ii) Adding a drop of water/ placing the sections in water- to keep the cells turgid and
prevent dehydration.
(iii) Staining- to make different parts of the cell more distinct and clear.
Stains commonly used are iodine solution, methylene/bromothymolblue, neutral red
and eosin.
©Gibson Gilbert
(iv) Mounting- Involves placing the specimen on the slide and then gently pressing it down
with a cover slip to flatten it.
Advantages of placing a coverslip over the specimen include: Holds the specimen in
place, protects specimen from drying up/dust particles and protects the objective lens.
(v) Fixation- Involves making the specimen hard or stiff for sectioning.
It can also be done after sectioning to maintain the structure of the specimen.
Commonly used fixative is 70% ethanol.
Note:
The stage should be kept dry for easy manipulation of specimen as wetness causes the slide
to stick onto the stage.
Plant materials are stiff enough and do not require fixation.
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(c) The diameter of the field of view is converted into micrometres.
(1millimetre=1000micrometres)
Practice question 2
Convert the diameter of the field of view in (b) above into micrometers. (1mk)
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(d) The ruler is then replaced with a prepared slide of onion epidermis.
(e) The cells along the diameter of the field of view are counted.
©Gibson Gilbert
Practice question 3
What is the number of cells along the diameter of the field of view in the figure below?
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(f) The diameter of the field of view (in micrometres) is divided by the number of cells along
the diameter of the field of view.
Practice question 4
Using your answers in (c) and (e) above, estimate the size of each cell. (2mks)
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Practice question 5
The diameter of the field of view was estimated to be 5mm under a certain magnification. 5
cells were observed along the diameter of the field of view. What was the diameter of one
cell in microns? (3mks)
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Cell specialization
This is the structural modification of a cell to perform a specific function.
©Gibson Gilbert
Specialized plant cells
Specialized Function Structural modification Diagram (to be drawn by the
plant cell learner)
Root hair cell Absorption of Has an extension called
water and the root hair to increase
mineral salts surface area for
from the soil absorption of water and
mineral salts from the soil
©Gibson Gilbert
Specialized animal cells
©Gibson Gilbert
Tissues
A tissue is a group/ collection of cells that are specialized to perform similar functions.
Animal tissues
Plant tissues
Epidermal tissue Protects inner tissues of plant from Consists of a single layer of
mechanical damage and entry of epidermal cells covering the outer
pathogens. surface of leaves and on young parts
of the stem and roots.
Palisade tissue Photosynthesis Has numerous chloroplasts which
contain chlorophyll to absorb light
energy for photosynthesis.
©Gibson Gilbert
Vascular tissue Xylem transports water and mineral Consists of cells that are
salts from the roots to the leaves. arranged/joined end to end
Phloem transports manufactured
food from the leaves to other parts
of the plant.
Meristematic Actively divide to allow growth. Found at growing regions of plants.
tissue Note: Consists of small, thin walled cells
Apical meristems bring about with no vacuoles but dense
primary growth. cytoplasm.
Lateral meristems bring about
secondary growth.
Parenchyma Provides mechanical support Consists of thin walled and
tissue Packaging and storage of food and irregularly shaped cells.
water
Organs
Examples of animal organs include: stomach, brain, kidney, liver, heart, eye and ear.
Organ system
Examples of animal organ systems include: digestive system, circulatory system, respiratory
system, excretory system, nervous system and reproductive system.
©Gibson Gilbert