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Legal Medicine 67 (2024) 102388

Contents lists available at ScienceDirect

Legal Medicine
journal homepage: www.elsevier.com/locate/legalmed

Differentiating suicide from homicide in sharp-force fatalities with stab


and/or incised wounds: A scoping review
Zainab Fathi AlGheryafi, Fatima Foud Alnasser, Fatima Hussain Almukhtar,
Fatema Abdullatef Aldajani, Fatimah Hussain Al Qassim, Zainab Mohammed Al Zakaria,
Shoq Obeid Alshammari, Ritesh G. Menezes *
College of Medicine, King Fahd Hospital of the University, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia

A R T I C L E I N F O A B S T R A C T

Keywords: The majority of sharp-force fatalities with stab and/or incised wounds are homicides. However, suicidal sharp-
Sharp-force injury force fatalities with stab and/or incised wounds are also reported. Thus, distinguishing suicidal stab and/or
Light sharp-edged weapon incised wounds from homicidal stab and/or incised wounds is significant from the forensic perspective. This
Manner of death
scoping review primarily summarizes the existing research findings on the differentiation of suicide from ho­
Autopsy
Forensic pathology
micide in sharp-force fatalities with stab and/or incised wounds. The literature was systematically searched on
February 28, 2023, using the PubMed database. A search string formed by a combination of keywords related to
suicide, homicide, and stab and incised wounds yielded 23 records. After applying the eligibility criteria, six
records/studies met the inclusion criteria and were included in the present scoping review. Results showed that
the predictive strength of various parameters, either individually or collectively, in diagnosing the manner of
sharp-force fatality as suicide or homicide is not always hundred percent accurate. Some of the important pre­
dictors of the homicidal manner of death in sharp-force fatalities include clothing damage, presence of defense
injuries, presence of injuries caused by another type of violence other than sharp-force, vertically oriented chest
stabs, and sharp-force injuries in the head and back anatomical sites. Some of the important predictors of the
suicidal manner of death in sharp-force fatalities include the presence of tentative injuries, sharp-force injuries to
the wrist, and the presence of a suicide note.

1. Introduction stabs. For instance, in Canada, over 25% of homicides were caused by
stabbing [4]. Suicidal incised wounds also form a small proportion of all
Fatalities from sharp-force injuries are commonly caused by stab, the suicides [3]. Comparatively, homicidal incised wounds are more
incised, or chop wounds. Stab and incised wounds result from light common than suicidal incised wounds [5]. The profile of injuries in
sharp-edged weapons. The manner of death in sharp-force fatalities is sharp-force fatalities may include only stab wounds or incised wounds,
often homicidal [1]. However, suicidal sharp-force fatalities are also or a combination of both stab and incised wounds as both can be caused
reported. by the same light sharp-edged weapon.
Suicidal stabbing is relatively uncommon when compared to homi­ Differentiating a suicide from a homicide is a central issue in forensic
cidal stabbing. For instance, an autopsy study from the United Kingdom pathology practice. Considering sharp-force fatalities with stab and/or
reported that the number of homicidal stabbings was nearly 8 times incised wounds, distinguishing suicidal wounds from homicidal wounds
more than suicidal stabbings [2]. Suicide by stabbing is a less common is significant from the forensic perspective. The differentiating process
method of committing suicide when compared to other methods of remains challenging to forensic pathologists as it is complicated with a
committing suicide. For instance, an autopsy study from Saudi Arabia weighty proportion of uncertainty, which makes performing a careful
reported that less than 5% of suicides were caused by stab wounds [3]. evaluation of all the components of a given case a must to distinguish a
On the other hand, a significant proportion of homicides are caused by suicide from a homicide. Therefore, we undertook this scoping review

* Corresponding author at: Forensic Medicine Division, Department of Pathology, College of Medicine, King Fahd Hospital of the University, Imam Abdulrahman
Bin Faisal University, Dammam 34212, Saudi Arabia.
E-mail addresses: mangalore971@yahoo.co.in, rgmenezes@iau.edu.sa (R.G. Menezes).

https://doi.org/10.1016/j.legalmed.2023.102388
Received 30 September 2023; Received in revised form 25 December 2023; Accepted 28 December 2023
Available online 28 December 2023
1344-6223/© 2023 Elsevier B.V. All rights reserved.
Z.F. AlGheryafi et al. Legal Medicine 67 (2024) 102388

primarily to summarize the existing research findings on the differen­ OR “sharp weapon”[Title] OR “scalpel”[Title] OR “sharp force”[Title]).
tiation of suicide from homicide in sharp-force fatalities with stab and/ The search identified 23 records linked to the aim of the present scoping
or incised wounds. Besides, an attempt is made to identify gaps in review about the characteristics differentiating suicide from homicide in
research and recommend future directions. sharp-force fatalities with stab and/or incised wounds.
All the identified records were screened according to the inclusion
2. Methods and exclusion criteria (Table 2). Initially, the title and abstract of the
identified records were screened by two researchers (the first two au­
The methodology for this scoping review included a systematic thors) independently. The year of publication was not considered as a
search to identify and screen relevant records/studies/articles for in­ criterion for selection of records. Only records published in English were
clusion (Fig. 1) [6]. PubMed was the database considered for the liter­ considered for selection. Records published in other languages, irre­
ature search. The search was performed on February 28, 2023. A spective of the availability of the translated version of the abstract, were
combination of keywords related to suicide, homicide, and sharp-force excluded. Accordingly, 3 records published in German [7–9], 1 in Polish
injury (stab/incised wound) was used to identify relevant studies/re­ [10], and 1 in French [11] were excluded.
cords (Table 1). The following final string of keywords was applied: Following the initial screening, 18 records were sought for retrieval
(“suicide”[Title] OR “suicidal”[Title] OR “self harm”[Title] OR “self”[­ of the full-text. The full-text of the retrieved records were assessed for
Title] OR “self injury”[Title]) AND (“homicide”[Title] OR “homi­ eligibility by two researchers (the first two authors) after being trained
cidal”[Title] OR “kill”[Title] OR “murder”[Title]) AND (“stab to do so by a senior researcher (the last author). Any disagreements
wound”[Title] OR “stab injury”[Title] OR “stab injuries”[Title] OR between the two researchers were resolved in consultation with the
“stabbing”[Title] OR “incised wound”[Title] OR “incised injur­ senior researcher. Records that reported non-fatal cases of attempted
ies”[Title] OR “cut”[Title] OR “sharp wound”[Title] OR “sharp injur­ suicide or attempted homicide by sharp-force were excluded [12]. Case
y”[Title] OR “sharp injuries”[Title] OR “knife”[Title] OR “thrust”[Title] reports that presented either a suicidal sharp-force fatality or homicidal

Fig. 1. Flow diagram depicting the identification and screening of records for inclusion (adapted from: http://prisma-statement.org/prismastatement/flowdiagram.
aspx).

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Z.F. AlGheryafi et al. Legal Medicine 67 (2024) 102388

Table 1
Database search strategy.
Suicide-related keywords Homicide-related keywords Sharp-force injury-related keywords Final search string of keywords

(“suicide”[Title] OR (“homicide”[Title] OR (“stab wound”[Title] OR “stab injury”[Title] OR (“suicide”[Title] OR “suicidal”[Title] OR “self


“suicidal”[Title] OR “self “homicidal”[Title] OR “stab injuries”[Title] OR “stabbing”[Title] OR harm”[Title] OR “self”[Title] OR “self injury”[Title])
harm”[Title] OR “self”[Title] “kill”[Title] OR “incised wound”[Title] OR “incised AND (“homicide”[Title] OR “homicidal”[Title] OR
OR “self injury”[Title]) “murder”[Title]) injuries”[Title] OR “cut”[Title] OR “sharp “kill”[Title] OR “murder”[Title]) AND (“stab
wound”[Title] OR “sharp injury”[Title] OR “sharp wound”[Title] OR “stab injury”[Title] OR “stab
injuries”[Title] OR “knife”[Title] OR injuries”[Title] OR “stabbing”[Title] OR “incised
“thrust”[Title] OR “sharp weapon”[Title] OR wound”[Title] OR “incised injuries”[Title] OR
“scalpel”[Title] OR “sharp force”[Title]) “cut”[Title] OR “sharp wound”[Title] OR “sharp
injury”[Title] OR “sharp injuries”[Title] OR
“knife”[Title] OR “thrust”[Title] OR “sharp
weapon”[Title] OR “scalpel”[Title] OR “sharp
force”[Title])

criteria and were included in the present scoping review [24–29]. The
Table 2
demographic profile and salient features of included articles are pre­
Inclusion and exclusion criteria.
sented in Table 3 and Table 4, respectively.
Inclusion criteria Another related critical review published in the year 2015 [20]
included diverse articles like reports that published separate data on
• Any publication date
• English language suicides and homicides, reports on attempted suicides or attempted
• Article type: Research articles homicides, reports on individual cases, and reports published in non-
• Study method: Postmortem examination/autopsy-based research [with or without English languages with the availability of the abstract in English, un­
other relevant data (example: crime scene investigation data, criminological data,
like the present scoping review. Besides, the literature search strategy
etc.)]
• Subject outcome: Fatal
adopted by De-Giorgio et al. [20] differed from the present scoping
• Manner of injuries: Suicidal and homicidal injuries review.
• Subject cohorts: Both victims of suicide and homicide
• Type of sharp-force injuries: Stab and/or incised wounds 3.2. Victim
• Stab wound: Stabs caused by light sharp-edged weapons
Exclusion criteria
3.2.1. Personal data
• Non-English language Both gender and age are considered noteworthy demographic data in
• Article type: Reviews, case reports, case series terms of the manner of death. The majority of the studies showed a
• Study method: Research based on crime scene investigation or criminological data, recognizable male predominance in both suicides and homicides. Ter­
but without postmortem examination/autopsy data
• Subject outcome: Non-fatal (attempted suicide, attempted homicide)
ranova et al. [24] reported that 85% of suicide victims and 58% of ho­
• Manner of injuries: Accidental and uncertain manner micide victims were male subjects. Similarly, Brunel et al. [25] reported
• Subject cohorts: Individual cohorts of victims of suicide or homicide that 83% of suicide victims and 67% of homicide victims were male
• Type of sharp-force injuries: Chop wounds subjects. Karlsson [29] reported a male/female ratio of 3.6:1 and 3.2:1
• Stab wound: Stabbing caused from penetration of the body by a blunt weapon
in the suicide group and homicide group, respectively. Although Scolan
(example: iron rod, etc.)
et al. [27] echoed a similar proportion of male victims (82%) in the
suicide group, the proportion of male victims in the homicide group was
sharp-force fatality with discussion on differentiation of a suicide from a 49%. A higher age of suicide victims when compared to homicide vic­
homicide were excluded [13–17]. Similarly, case series were excluded tims was found in most studies. In the cohort analyzed by Terranova
[18,19]. All types of review articles without any primary data reported et al. [24], the median (interquartile range) age in years for the suicide
were excluded [20]. Records that included cases of accidental or un­ victims and homicide victims was 45 (37.75–54.5) and 43.5 (35.25–53),
certain manner of injuries by sharp-force in addition to cases of suicidal respectively. Brunel et al. [25] reported a mean age of 48.2 years for
sharp-force injuries and homicidal sharp-force injuries were excluded suicide victims and 40.2 years for homicide victims. Similarly, Scolan
[21]. Records that included cases of chop wounds in the profile of sharp- et al. [27] reported a significantly higher mean age of suicide victims
force injuries while differentiating suicides from homicides were (52.8 years) than that of homicide victims (38.9 years). Karlsson [29]
excluded because the profile of the weapons causing chop wounds dif­ reported the mean age separately for male subjects and female subjects
fers from the weapons causing stab and/or incised wounds in being in each category of the manner of death. Significant differences in ages
heavy versus light [22]. Records that included cases of dyadic deaths were found both between male suicide (mean, 51.5 years) and homicide
due to sharp-force injuries were excluded [23]. Non-autopsy-based victims (mean, 37.1 years) and between female suicide (mean, 46.0
studies (without any data on postmortem examination findings) on years) and homicide victims (33.8 years) [29]. However, demographic
sharp-force injuries were originally meant to be excluded. However, no data on the gender and age of the suicide and homicide victims was not
such study was identified. categorically reported by Racette et al. [26].
In a related critical review by De-Giorgio et al. [20], the calculated
3. Results and discussion male/female ratio was 3.45:1 in the suicide group. However, the
calculated male/female ratio was 0.32:1 in the homicide group (female:
The narrative structure of the ‘results and discussion’ section is as male = 3.13:1) [20]. This proportion of males and females in the ho­
follows: the first paragraph under each sub-section is related to the micide group is in contrast to the findings reported in the studies
‘results’ aspect and the second paragraph under each sub-section is included in the present scoping review. The calculated mean age was
related to the ‘discussion’ aspect. 46.23 years and 39.11 years in the suicidal and homicidal sharp-force
fatalities, respectively [20], similar to the higher age of suicide vic­
3.1. Profile of the included articles tims reported in the studies included in the present scoping review.

After applying the eligibility criteria, six studies met the inclusion

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Z.F. AlGheryafi et al. Legal Medicine 67 (2024) 102388

Table 3
Demographic profile of articles that met the inclusion criteria.
Reference Year of Authors Journal name Study location Years of data included in the
publication study

[24] 2020 Claudio Journal of Forensic Sciences Institute of Legal Medicine 1997–2019 (23-year period)
Terranova, et al. of the University of Padua, Italy

Department of Legal
Medicine in the Health Unit of Venice, Italy
[25] 2010 Christophe Forensic Science International Department of Forensic Medicine and Pathology at 1986–2008 (23-year period)
Brunel, et al. the Raymond Poincare Hospital, Garches, France
[26] 2008 Stephanie Forensic Science Medicine and Quebec Province, Canada 5-year period*
Racette, et al. Pathology
[27] 2004 V. Scolan, et al. American Journal of Forensic Toulouse Region, France 10-year period*
Medicine and Pathology
[28] 1998 Thore Karlsson Forensic Science International Department of Forensic Medicine in Stockholm, 1983–1992 (10-year period) &
Sweden 1993-1995 (3-year period)
[29] 1998 Thore Karlsson Forensic Science International Department of Forensic Medicine in Stockholm, 1983–1992 (10-year period)
Sweden

*Specific years – not mentioned.

3.2.2. Psychiatric history suicides and 83.3% of homicides. The weapon was recovered near or
The presence of a history of a clinical psychiatric diagnosis raises the inside the body in 85% and 56.6% of suicides and homicides, respec­
suspicion of suicide. Terranova et al. [24] reported that 70% of the tively [24]. A kitchen knife was the most commonly used light sharp-
suicide victims had a history of clinical psychiatric diagnosis, and 50% edged weapon in both suicides (70%) and homicides (80%) [24].
of them were on treatment with pharmacological therapy. Karlsson [29] Karlsson [29] reported that kitchen knives (33.33%) and razor blades
reported a history of suicidal ideation and previous suicide attempts in (30.48%) were the most frequently used light sharp-edged weapons in
53% and 27% of the suicide victims, respectively. However, Brunel et al. suicides. In homicides, kitchen knives were used in 38.51% of the cases,
[25], Racette et al. [26], and Scolan et al. [27] did not report on the and razor blades were not used even in a single case [29]. The use of
psychopathology of the victims. weapon-type knives (e.g., stiletto, dagger, bayonet) was significantly
In a related critical review by De-Giorgio et al. [20], a psychiatric higher in homicides than suicides [29]. However, Brunel et al. [25],
history of previous suicidal attempts was positive in 20.38% of suicide Racette et al. [26], and Scolan et al. [27] did not report the site of re­
victims and absent in 79.62% of suicide victims where the related in­ covery of the weapon.
formation was available. Moreover, a history of clinical psychiatric In a related critical review, De-Giorgio et al. [20] did not report
diagnosis was present in 49.31% of the suicide victims and absent in findings related to the site of recovery of the weapon. The recovery of
50.69% of the suicide victims where the related information was the light sharp-edged weapon beside the victim’s body may indicate a
available [20]. Depression followed by schizophrenia were the two most suicide, but not necessarily always because the murder weapon may be
common clinical psychiatric diagnoses reported [20]. left behind near the victim’s body by the murderer to simulate suicide
[33]. On the other hand, the absence of the weapon from the scene of
3.2.3. Suicide notes death may indicate a homicide [34]. In suicides, household sharp-edged
The presence of a suicide note may indicate the victim’s motive in objects like the kitchen knife are commonly used. In homicides, the
cases of suicide. Terranova et al. [24] reported that 50% of the suicide murder weapon may vary from kitchen knives to weapon-type knives.
victims had written a suicide note explaining the reasons to commit
suicide. Karlsson [29] reported that 18% of the suicide victims had left a 3.3.2. Blood traces
written suicide note. However, Brunel et al. [25], Racette et al. [26], and Terranova et al. [24] reported that traces of blood far away from the
Scolan et al. [27] did not report the presence or absence of suicide notes body were found in only 10% of suicides. However, traces of blood far
in cases of suicide. away from the body were found in nearly half (47%) of the cases of
When present, a suicide note indicates suicide. Nevertheless, it homicide [24]. Blood on the victim’s palms was found in 70% and
should be noted that a false suicide note may be planted by the murderer 63.3% of suicides and homicides, respectively [24]. However, Brunel
to simulate suicide. In a related critical review, De-Giorgio et al. [20] did et al. [25], Racette et al. [26], Scolan et al. [27], and Karlsson [29] did
not analyze suicide notes. Not all suicide victims leave behind a suicide not report on the scene of death investigation findings related to
note [30,31]. The presence of a suicide note is a predictor of the manner evidentiary traces of blood.
of death being a suicide. However, the absence of a suicide note does not In a related critical review, De-Giorgio et al. [20] did not report
exclude suicide as the manner of death. scene-of-death examination findings related to evidentiary blood traces.
The absence of distant blood traces may indicate a suicide, but not
necessarily always. Pelletti et al. [13] reported an unusual case of sui­
3.3. Scene of death cidal stabbing disguised by the victim as a homicide by alteration of the
scene of death during the last minutes of life. Distant evidentiary traces
Scene of death investigation is important in determining the manner of blood were recovered in this case of self-stabbing [13].
of death [32]. Examination of the scene of death provides possible
predictors of suicides and homicides. Such predictors include the pres­
ence of a suicide note, the site of recovery of the weapon, and the site of 3.4. Necroscopic findings
blood traces. The presence of an evidentiary suicide note at the scene of
death in cases of suicide is discussed under section 3.2.3. Here, the site of Forensic autopsy plays a key role in the determination of the manner
recovery of the weapon and blood traces are reported and discussed. of death [35]. Forensic autopsy findings include the findings of the ex­
amination of the clothes, examination of the body, and autopsy ancillary
3.3.1. The weapon investigations [36]. In this section, damage to victim’s clothing, wound
Terranova et al. [24] reported that the weapon was found in all characteristics, associated injuries, and postmortem toxicological

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Z.F. AlGheryafi et al. Legal Medicine 67 (2024) 102388

Table 4
Salient features of the studies that met the inclusion criteria.
Reference -Type of study Overall cases included in the study Study objective Take home message
-Documents
reviewed

Terranova -Retrospective 51 cases of sharp-force fatalities (20 suicides To identify relevant parameters to -In most cases of suicidal and homicidal sharp-force
et al., 2020 study and 31 homicides) distinguish between suicidal and fatalities, differentiation is achievable through an
- Autopsy and homicidal sharp-force fatalities integrated evaluation of postmortem external
[24] allied reports* examination and death scene investigation findings.
-This differentiation is not possible in all cases.
-Examination of victim’s clothes for damage is of
paramount importance in differentiating suicidal and
homicidal sharp-force fatalities
Brunel et al., -Retrospective 118 cases of sharp-force fatalities (48 To identify reliable parameters to -Differentiation of suicidal and homicidal sharp-force
2010 study suicides and 70 homicides) differentiate suicidal and homicidal fatalities is achievable through an integrated
-Autopsy reports sharp-force fatalities evaluation of autopsy findings and death scene
[25] investigation findings.
-This differentiation is not possible in all cases.
-Hesitation injuries are common in suicidal sharp-
force fatalities.
-Defense injuries are common in homicidal sharp-
force fatalities.
Racette et al., -Retrospective 207 cases of sharp-force fatalities (58 To compare features of hesitation - Differentiation of suicidal and homicidal sharp-
2008 study suicides and 149 homicides) injuries and defense injuries in force fatalities is achievable through an evaluation of
-Autopsy and suicidal and homicidal sharp-force hesitation injuries and defense injuries.
[26] allied reports* fatalities -Hesitation injuries are more commonly found on the
anterior aspect of upper limbs.
-Defense injuries are found on the anterior aspect as
much as the posterior aspect of upper limbs.
-Hesitation injuries are often grouped.
-Defense injuries are often dispersed on upper limbs.
-A smaller number of defense injuries are linked to a
higher blood alcohol concentration in the victim.
Scolan et al., -Retrospective 70 cases of stabbings (17 suicides and 53 To identify variables that enable to -In stabbings, the presence of several wounds, the
2004 study homicides) meaningfully differentiate suicidal victim’s clothing damage, and vertically oriented
-Autopsy and and homicidal stabbing fatalities chest wounds are suggestive of homicide.
[27] allied reports*

Karlsson, -Retrospective − 279 cases of sharp-force fatalities (105 To develop models for -The models developed cannot replace a forensic
1998 study suicides and 174 homicides) – MODEL SET discrimination between suicidal and pathologist but may be used as an aiding tool by the
-Autopsy and created by multivariate projective statistical homicidal sharp-force fatalities forensic pathologist.
[28] allied reports* methods and logistic regression analysis -Predictors of homicidal manner of death in sharp-
− 67 cases of sharp-force fatalities (27 force fatalities: clothing damage, blood alcohol level,
suicides and 40 homicides) – TEST SET presence of defense injuries, presence of injuries
validation caused by another type of violence other than sharp-
force, vertically oriented chest stabs, upper extremity
sharp-force injuries (except wrist and front of the
elbow), head and back sharp-force injuries
-Predictors of suicidal manner of death in sharp-force
fatalities: presence of tentative injuries, history of
suicidal ideation, wrist sharp-force injuries, presence
of suicide note, sharp-force injuries to the front of the
elbow
Karlsson, -Retrospective 279 cases of sharp-force fatalities (105 To identify variables of importance -In sharp-force fatalities, a higher number of wounds
1998 study suicides and 174 homicides) in the differentiation between in the head and lower extremities, and a higher
-Autopsy and suicidal and homicidal sharp-force number of vertical chest stab wounds are suggestive
[29] allied reports* fatalities of homicide.

*
Allied reports – example: police investigation reports, autopsy ancillary investigation reports (e.g. toxicology reports), etc.

analysis findings are reported and discussed. Besides, a section on and 79% of homicides. However, clothing description was not reported
postmortem radiology is included. by Brunel et al. [25] and Racette et al. [26].
Victim’s clothing may play a crucial role in differentiating suicides
3.4.1. Clothing from homicides. In a related critical review by De-Giorgio et al. [20],
Terranova et al. [24] reported that the presence of clothing damage clothing damage was absent in 76.55% of suicides and present in
(injuries to clothing) caused by the light sharp-edged weapon was 23.45% of suicides where the information on clothing was available. In
directly associated with homicide. Clothing damage was present in 20% cases where this information was available, the corresponding pro­
and 71% of the suicides and homicides, respectively [24]. Scolan et al. portions for homicides were 25.91% and 74.09%, respectively [20].
[27] reported significantly more frequent clothing damage (presence of
slashes) in homicides. The absence of clothing or raised clothing in the 3.4.2. Wound characteristics
region of the stabs indicates suicide [27]. Karlsson [29] reported a sig­
nificant difference in the proportion of clothing damage wherein injuries 3.4.2.1. Type of wounds. Brunel et al. [25] reported that incised wounds
to clothing in association with wounds were present in 5% of suicides occurred preferentially in suicides (39.6% of suicides versus 8.6% of

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Z.F. AlGheryafi et al. Legal Medicine 67 (2024) 102388

homicides). The presence of both stab and incised wounds occurred abdomen, and forearms to the exclusion of other anatomical regions
preferentially in homicides (35.7% of homicides versus 16.7% of sui­ [25]. A higher likelihood of homicidal manner of death was predicted if
cides) [25]. The presence of injuries caused by blunt impact in associ­ wounds were located in anatomical regions constituting the head, nape
ation with sharp-force injuries was seen only in homicides (7.1% of of the neck, limbs, and hands to the exclusion of other anatomical re­
homicides versus 0% of suicides) [25]. Stab wounds alone were found in gions [25]. In the study by Karlsson [29], a significant difference was
43.8% and 48.6% of suicides and homicides, respectively [25]. The noted in suicides and homicides when the head, anterior aspect of the
presence of associated fall-related injuries was not a significant param­ abdomen, and lower extremities were the anatomical sites of wounds.
eter in differentiating suicides and homicides [25]. Karlsson [29] re­ The head was the anatomical site of wounds in 1.90% and 27.01% of
ported the presence of other types of injuries (blunt-impact injuries, suicides and homicides, respectively [29]. The abdomen was the
firearm injuries, or contusions from attempted strangulation) in 28% of anatomical site of wounds in 9.52% and 26.44% of suicides and homi­
homicides. Injuries caused by another type of violence were reported in cides, respectively [29]. The lower extremities were the anatomical site
7% of suicides [29]. In the study by Scolan et al. [27], it was found that of wounds in 2.86% and 18.97% of suicides and homicides, respectively
the presence of other types of injuries in association with stab wounds [29]. Suicidal wounds were not found in the region of the back and
did not provide significant prediction of the manner of death. Terranova genitals [29]. In the study by Scolan et al. [27], it was found that the
et al. [24] and Racette et al. [26] did not report on the predominance of a anatomical site of wounds did not provide a significant prediction of the
type of injury (stab wounds only, incised wounds only, both stab and manner of death. Racette et al. [26] did not report on the predictive
incised wounds, or both blunt-impact injuries and sharp-force injuries) value of the anatomical site of wounds in classifying the manner of death
by the manner of death (suicide or homicide). as suicide or homicide.
In a related critical review, De-Giorgio et al. [20] did not report the The anatomical site of wounds is a strong predictive parameter of the
association of types of injuries by the manner of death. Oshima and manner of death (suicide or homicide), according to some studies. In a
Kondo [19] did not find injuries caused by other types of violence in the related critical review by De-Giorgio et al. [20], chest (36.2%), upper
8 cases of sharp-force fatalities reported by them. limbs (24.4%), and abdomen (18.3%) were identified as the top three
anatomical sites of suicidal wounds when the related information was
3.4.2.2. Number of wounds. In the study by Terranova et al. [24], the available. The chest (54.1%), lower limbs (16.1%), and abdomen
median (interquartile range) values of the total number of wounds (13.3%) were identified as the top three anatomical sites of homicidal
(excluding hesitation injuries in suicides and defense injuries in homi­ wounds when the related information was available [20]. The lower
cides) were 3 (1–5.25) and 7 (2.5–15) in suicides and homicides, limbs were the anatomical sites of wounds in only 0.8% of suicides [20].
respectively. Brunel et al. [25] reported that the total number of wounds The back was the anatomical site of wounds in 0% and 1.4% of suicides
was not a significant predictive parameter in classifying the manner of and homicides, respectively [20]. The head was the anatomical site of
death as suicide or homicide. The mean (±S.D.) of the number of wounds in 3.1% and 5.7% of suicides and homicides, respectively [20].
wounds was 8.6 (±18.8) and 11.5 (±14.7) in suicides and homicides, The neck was the anatomical site in 17.2% and 9.3% of suicides and
respectively, with a wide range of overlap between suicides and homi­ homicides, respectively [20].
cides [25]. Besides, the quantification of wounds (number of wounds ≤
3 versus number of wounds > 3) did not provide a significant prediction 3.4.2.4. Orientation of wounds. Brunel et al. [25] reported that the
of the manner of death [25]. However, Scolan et al. [27] reported that longitudinal axis of stab wounds situated at the anterior aspect of the
the total number of wounds differed significantly between suicides and trunk (chest and abdomen) was a significant predictive parameter in
homicides, with the mean (±S.D.) values of 2.5 (±0.48) and 11 (±1.75) classifying the manner of death as suicide or homicide. A higher likeli­
in suicides and homicides, respectively. Karlsson [29] reported a sig­ hood of homicidal manner of death was predicted when the axis of stab
nificant difference in the total number of wounds between suicides and wounds was vertical [25]. In the study by Scolan et al. [27], a significant
homicides when vertical chest stab wounds were compared. The corre­ difference in the presence of vertical chest stab wounds in suicides and
sponding mean values were 0.09 (±0.37) and 0.70 (±1.39) for suicides homicides was reported. Vertical chest stab wounds were not found in
and homicides, respectively [29]. However, the difference in the total suicides, whereas such wounds were frequent in homicides [27].
number of horizontal chest stab wounds between suicides and homicides Karlsson [29] reported a significant difference in the number of vertical
was insignificant [29]. Racette et al. [26] did not report on the predic­ chest stab wounds in suicides and homicides. However, the difference in
tive value of the total number of wounds in classifying the manner of the number of horizontal chest stab wounds in suicides and homicides
death as suicide or homicide. was insignificant [29]. Terranova et al. [24] and Racette et al. [26] did
In a related critical review, De-Giorgio et al. [20] reported the mean not report on the predictive value of the axis of stab wounds in differ­
of the number of injuries to be 2.4 and 3.8 in suicides and homicides, entiating a suicide from a homicide.
respectively. The corresponding S.D. was not provided [20]. Occasion­ In a related critical review, De-Giorgio et al. [20] did not report re­
ally, cases with a large number of stab wounds are reported in suicidal sults related to the orientation of wounds. However, De-Giorgio et al.
stabbings. Rautji et al. [37] reported a case of suicidal stabbing with 15 went on to discuss the importance of the axis of wounds in differenti­
cavity-deep stab wounds to the chest. ating the manner of death as suicide or homicide [20]. It is assumed that
the horizontal orientation of wounds is more common in suicidal sharp-
3.4.2.3. Anatomical sites of wounds. In the study by Terranova et al. force fatalities than in homicidal sharp-force fatalities [20].
[24], the common anatomical sites of suicidal wounds included the
anterior aspects of the neck, abdomen, and left side of the chest. The 3.4.2.5. Bone or cartilage wounds. Brunel et al. [25] reported that the
most common anatomical sites of homicidal wounds included the presence of bone or cartilage wounds was a significant predictive
anterior aspect of the neck, the anterior aspect of the left side of the parameter in classifying the manner of death. A higher likelihood of
chest, the back, and the anterior aspect of the abdomen [24]. The head homicidal manner of death was predicted when bone or cartilage
was the anatomical site of wounds in homicides but did not feature in wounds were found [25]. A higher likelihood of suicidal manner of
suicides [24]. Brunel et al. [25] reported that the distribution of wounds death was predicted when bone or cartilage wounds were absent [25].
by anatomical sites was a significant predictive parameter in classifying Karlsson [29] reported a more common incidence of rib and sternal
the manner of death as suicide or homicide. A higher likelihood of sui­ wounds in homicides in comparison to suicides. Terranova et al. [24],
cidal manner of death was predicted if wounds were located in Racette et al. [26], and Scolan et al. [27] did not report bone or cartilage
anatomical regions constituting the anterior aspects of the neck, chest, wounds in differentiating a suicide from a homicide.

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In a related critical review, De-Giorgio et al. [20] did not report re­ sustained when the victim tries to grab the weapon in defense. Defense
sults on bone or cartilage wounds in distinguishing a suicide from a wounds, when present in homicides, are unlikely to be fatal. Defense
homicide. It is assumed that suicidal victims of sharp-force injuries tend injuries, when present, are a feature of homicidal victims. However, odd
to avoid wounding solid anatomical structures like bones [38]. cases of injuries mimicking defense injuries are very rarely reported in
suicides [24]. In a related critical review by De-Giorgio et al. [20], de­
3.4.2.6. Injury severity score. Brunel et al. [25] reported that the injury fense injuries were present in 37.39% of homicidal victims and absent in
severity score (ISS) was a significant predictive parameter in classifying 62.61% of homicidal victims where the information on defense injuries
the manner of death as suicide or homicide. A higher likelihood of ho­ was available.
micidal manner of death was predicted with increasing ISS [25].
Conversely, a lower likelihood of suicidal manner of death was predicted 3.4.4. Toxicology
with increasing ISS [25]. The mean (±S.D.) of the ISS was 15.73 (±8.34) In the study by Scolan et al. [27], toxicological analysis of blood was
and 29.17 (±13.27) in suicides and homicides, respectively [25]. The negative for alcohol in 73.3% of suicides and 77.8% of homicides where
ISS ranged from 1 to 29 and 10 to 75 in suicides and homicides, such analysis was conducted. The differences in these findings were not
respectively [25]. None other researchers reported on the predictive statistically significant in the suicide and homicide sub-cohorts [27].
value of the ISS in differentiating suicide from homicide in sharp-force Karlsson [29] reported significantly higher blood alcohol levels in ho­
fatalities. micides in comparison to that in suicides. Around 15% of the suicide
The ISS is one of the anatomical scoring systems used to assess the victims and around 60% of the homicide victims were found to be under
severity of traumatic injuries [39,40]. Brunel et al. [25] were the first to the influence of alcohol at the time of death [29]. Racette et al. [26]
analyze the effect of the ISS in distinguishing suicidal sharp-force fa­ analyzed the correlation between blood alcohol concentration and the
talities from homicidal sharp-force fatalities. The sharp-force injuries presence of hesitation injuries in suicidal victims and defense injuries in
caused by assailants in homicides were more severe than self-inflicted homicidal victims. This study did not reveal any correlation between
sharp-force injuries [25]. In a related critical review, De-Giorgio et al. blood alcohol concentration and the presence of hesitation injuries [26].
[20] did not report results related to the ISS in distinguishing a suicide However, this study revealed a negative correlation between blood
from a homicide. alcohol concentration and the presence of defense injuries [26]. Defense
injuries were absent in every case of homicide where toxicological
3.4.3. Associated injuries analysis found a blood alcohol concentration of more than 250 mg/100
In addition to the fatal wounds or primary wounds inflicted to ml [26]. Overall, a higher blood alcohol concentration was linked to a
commit suicide or homicide, secondary associated injuries like hesita­ smaller number of defense injuries in homicidal victims [26]. Around
tion injuries and defense injuries may be present predominantly in sui­ 80% of the homicidal victims with a single fatal wound and absence of
cides and homicides, respectively. defense injuries were found to be under the influence of alcohol at the
time of death [26]. However, around 90% of the suicidal victims with a
3.4.3.1. Hesitation injuries. Terranova et al. [24], Karlsson [29], and single fatal wound and absence of hesitation injuries were found not to
Racette et al. [26] reported the presence of hesitation injuries or be under the influence of alcohol at the time of death [26]. Terranova
tentative injuries in 50%, 62%, and 74.14% of suicidal victims, et al. [24] and Brunel et al. [25] did not report on toxicological analysis
respectively. Brunel et al. [25] reported that hesitation injuries were due to the unavailability of data in most cases.
preferentially found in suicidal victims. In the study by Terranova et al. In a related critical review by De-Giorgio et al. [20], toxicological
[24], the common anatomical sites where hesitation injuries were pre­ analysis results were positive in 88 cases of suicide and negative in 132
sent included the anterior surface of the forearms and neck. In the study cases of suicide where the related information was available. In homi­
by Racette et al. [26], the common anatomical sites where hesitation cides, toxicological analysis results were positive in 144 cases and
injuries were present included the neck, left anterior thorax, wrists, and negative in 91 cases where the related information was available [20].
hands. Scolan et al. [27] did not report on the presence or absence of However, De-Giorgio et al. did not comment further on the positivity of
hesitation injuries in suicidal victims. the toxicological analysis results. A positive toxicological analysis report
In a suicidal milieu, hesitation injuries are superficial injuries. Hes­ in suicidal victims may indicate a preceding failed suicidal attempt by
itation injuries are non-fatal injuries that may be present in suicidal poisoning or the employment of an additional method of suicide by
sharp-force fatalities. Hesitation injuries, when present, are a feature of poisoning to ensure a successful suicide. Besides, toxicological analysis
suicidal victims. However, odd cases of injuries mimicking hesitation may be positive for medications for a clinical psychiatric diagnosis in
injuries are very rarely reported in homicides [29]. In a related critical those suicidal victims with a history of clinical psychiatric diagnosis and
review by De-Giorgio et al. [20], hesitation injuries were present in on pharmacological treatment for the clinical psychiatric diagnosis. A
62.4% of suicidal victims and absent in 37.6% of suicidal victims where positive toxicological analysis report in homicidal victims may indicate
the information on hesitation injuries was available. being inebriated or drugged. In the studies included in the present
scoping review [26,27,29], postmortem blood samples were analyzed
3.4.3.2. Defense injuries. Terranova et al. [24], Racette et al. [26], and for toxicological findings. Blood is the most common biological sample
Karlsson [29] reported the presence of defense injuries in 64.51%, analyzed for alcohol, drugs, or substances in sharp-force fatalities [41].
61.07%, and 41% of homicidal victims, respectively. Brunel et al. [25] Other biological samples [42–44], such as urine, viscera samples, and
reported that defense injuries were preferentially found in homicidal hair, were not analyzed. Validated analytical methods [42–44] that
victims. In the study by Racette et al. [26], the common anatomical sites include thin-layer chromatography, high-performance liquid chroma­
where defense injuries were present included the hands, arms, and tography, and gas chromatography-mass spectrometry are used to
forearms. Localizations of defense injuries were not reported by Terra­ analyze postmortem biological samples for toxicological findings.
nova et al. [24], Brunel et al. [25], and Karlsson [29]. Scolan et al. [27]
did not report on the presence or absence of defense injuries in homi­ 3.4.5. Radiology
cidal victims. Radiological imaging modalities play an important role in the post­
In a homicidal milieu, defense injuries are injuries to the extremities. mortem setting as one of the autopsy ancillary investigations [45]. None
Defense injuries are categorized as passive defense injuries and active of the studies included in the present scoping review specifically re­
defense injuries. Passive defense injuries are sustained when the victim ported postmortem radiological findings related to sharp-force skeletal
raises the upper limbs in defense, and active defense injuries are trauma. Such findings were not stated even in a related critical review by
De-Giorgio et al. [20].

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Z.F. AlGheryafi et al. Legal Medicine 67 (2024) 102388

Postmortem radiological imaging modalities can be used to detect region of the fatal sharp-force injury. Accordingly, predictors of suicide
sharp-force skeletal trauma [46]. Skeletal trauma may be missed on a and homicide should be analyzed separately for well-defined cohorts,
routine postmortem examination in regions that are difficult to reach for instance, cohort that includes cases of cut-throat fatalities and cohort
during autopsy [47]. Postmortem computed tomography (CT) scans that includes cases of fatal chest stabbings. Besides, we recommend a
easily detect skeletal injuries [48]. CT scans are more sensitive than X- meta-analytic review of the frequency and patterning of various relevant
rays in detecting stab-related osteological defects and the minimum parameters to provide the evidence in differentiating suicides from ho­
number of impacts [49]. When radiological evaluation of sharp-force micides in sharp-force fatalities caused by light sharp-edged weapons.
skeletal trauma is required, CTs should be considered over X-rays. Funding
Further inspection based on the location of sharp-force soft-tissue in­ Nil.
juries helps in identifying sharp-force skeletal injuries. As an alternative Ethics approval
to routine dissection in certain scenarios or as a supplement to routine Not applicable.
dissection, radiological imaging can be considered in the postmortem
setting to identify sharp-force osteological defects. However, the
detection of sharp-force skeletal trauma needs expertise in radiology and Declaration of Competing Interest
could be challenging to forensic pathologists untrained in radiology.
The authors declare that they have no known competing financial
interests or personal relationships that could have appeared to influence
4. Conclusion
the work reported in this paper.
This scoping review primarily summarizes the existing research
findings on the differentiation of suicide from homicide in sharp-force Acknowledgement
fatalities with stab and/or incised wounds. Scene of death investiga­
tion findings and autopsy findings are of paramount significance in None
establishing the manner of death. Several parameters that could be
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