1. An amino acid with pKa values of 2.2, 9.0 and 10.0, which of the amino acid would be?

A)
Lysine, B) Aspartate, C) Leucine, D) Serine, E) Proline

2. What is the sequence of the following peptide?

A) Ser-Asp-Gln-His-Arg B) Thr-Glu-Asn-Trp-Lys
C) Ser-Glu-Asn-Trp-Lys D) Thr-Glu-Asn-His-Lys
E) Ser-Glu-Asn-His-Lys

3. Which of the following peptides is more likely to take up an α-helical structure?

(A) Ala-Gly-Cys-Arg-Gly-Asn-Asp-Pro-Gln-Phe-Pro-Trp-Gly-Thr
(B) Cys-Arg-Ala-Gly-Gly-Phe-Pro-Trp-Gly-Thr-Asn-Asp-Pro-Gln
(C) Leu-Lys-Ala-Glu-Asn-Ala-Ala-Ala-Arg-Ala-Met-Ser-Glu-Ala
(D) Glu-Asn-Ala-Ala-Pro-Gly -Arg-Gly-Asn-Asp-Gln-Phe-Pro-Trp

4. The major carrier of chemical energy in all cells is:

A) acetyl triphosphate.
B) adenosine monophosphate.
C) adenosine triphosphate.
D) cytosine tetraphosphate.
E) uridine diphosphate.

5. The three-dimensional structure of macromolecules is formed and maintained primarily through

noncovalent interactions. Which one of the following is not considered a noncovalent interaction?
A) carbon-carbon bonds
B) hydrogen bonds
C) hydrophobic interactions
D) ionic interactions
E) van der Waals interactions

6. Of the 20 standard amino acids, only ___________ is not optically active. The reason is that its
side chain ___________.

A) alanine; is a simple methyl group

B) glycine; is a hydrogen atom
C) glycine; is unbranched
D) lysine; contains only nitrogen
 E) proline; forms a covalent bond with the amino group

7. Titration of valine by a strong base, for example NaOH, reveals two pK’s. The titration reaction
occurring at pK2 (pK2 = 9.62) is:
A) —COOH + OH  —COO + H2O
B) —COOH + —NH2  —COO + —NH2+
C) —COO + —NH2+  —COOH + —NH2
D) —NH3+ + OH  —NH2 + H2O

E) —NH2 + OH  —NH + H2O

8. Prosthetic groups in the class of proteins known as glycoproteins are composed of:
A) carbohydrates.
B) flavin nucleotides.
C) lipids.
D) metals .
E) phosphates.

9. Which of the following statements is false?

A) Collagen is a protein in which the polypeptides are mainly in the -helix conformation.
B) Disulfide linkages are important for keratin structure.
C) Gly residues are particularly abundant in collagen.
D) -keratin is a protein in which the polypeptides are mainly in the -helix conformation.

10. The predominant structural feature in myosin molecules is:

A) a structure.
B) an helix.
C) the Fab domain.
D) the light chain.
E) the meromyosin domain.

11. Which of the following is a homotropic effector of hemoglobin-oxygen binding?

A) O2
B) H+
C) CO2
D) Cl-
E) 2,3-bisphosphoglycerate (2,3-BPG)
12. Given the following plot of oxygen binding versus pressure of
O2 for myoglobin (A) and hemoglobin (B, C, D), indicate a
curve that shows what happens when the pH is relatively the
lowest. D

13. What type of secondary structure is most prominent in myosin?

A) antiparallel -sheet
B) parallel -sheet
C) -helix
D) 310 helix
E) polyproline helix

14. In muscle, thin filaments are made of _______ while thick filaments are made of _______.
A) myosin; actin
B) actin; myosin
C) myofibril; myofiber
D) myofiber; myofibril
E) none of the above

15. Which of the following only occurs when ATP (not ADP) is bound to the myosin head?
A) actin is released from its binding site on myosin
B) weak binding of myosin head to actin
C) strong binding of myosin head to actin
D) power stroke of muscle contraction
E) none of the above

16. Microtubules are made from which of the following proteins?

A) actin
B) myosin
C) tubulin
D) cytoplasmic dynein
E) kinesin

Which of the following atoms could interact through a hydrogen bond?

A) the oxygen of a ketone and the hydrogen of an aldehyde
 B) the oxygen of methanol and a hydrogen on the methyl carbon of methanol
C) the hydrogen of an amine and the oxygen of an alcohol
D) the hydrogen on an aromatic ring and the oxygen of carbon dioxide

2 Amino acid-X contains two carboxylic acid groups (pKa values of 2.2 and 4.2) and an amine group
(pKa 9.7). What is the pI for amino acid-X?
A) 3.2
B) 6.0
C) 6.5
D) 7.0

3 α-Keratin hair grows at a rate of 20 cm/yr. Calculate the rate at which peptide bonds of α-keratin
chains must be synthesized (amino acid residue number per second) to account for the observed
yearly growth of hair. (計算每秒合成多少胺基酸數目) (Hint: The structure α-helix has 3.6
amino acid residues per turn and a rise of 5.4 Å per turn.). (A) 7 (B) 14 (C) 21 (D) 42

Consider the following amino acid sequence.

4 Where might bends most likely to occur when peptide folds? (hint:摺疊轉彎處)
(A) Residue 3
(B) Residue 7
(C) Residue 13
(D) Residue 21

5 Where might intrachain cross-linkages be formed? (A) Residue 4 and 16

(B) Residue 16 and 28 (C) Residue 13 and 24 (D) Residue 12 and 22

6 Which of the following is not correct concerning cooperative binding of a ligand to a protein?
(A) It is usually a form of allosteric interaction (B) It is usually associated with proteins with
multiple subunits (C) It results in a sigmoidal binding curve (D) It rarely occurs in enzymes

7 Which of the following peptides is more likely to take up an

α-helical structure?
(a) Leu-Lys-Ala-Glu-Asn-Ala-Ala-Ala-Arg-Ala-Met-Ser-Glu-Ala
(b) Cys-Arg-Ala-Gly-Gly-Phe-Pro-Trp-Gly-Thr-Asn-Asp-Pro-Gln
(C) Ala-Gly-Cys-Arg-Gly-Asn-Asp-Pro-Gln-Phe-Pro-Trp-Gly-Thr
(d) Glu-Asn-Ala-Ala-Pro-Gly -Arg-Gly-Asn-Asp-Gln-Phe-Pro-Trp

A 100 mL solution of 0.1 M glycine at pH 1.72 was titrated with 2 M NaOH solution. The pH was
monitored and the results were plotted as shown in the following graph. The key points in the
titration are designated I to V.

(A) I; (B) II; (C) III; (D) IV; (E) V;

8 The average net charge of glycine is zero.

9 The predominant species is ＋H3N+-CH2-

COOH.

10 Glycine has its maximum buffering

capacity.

11 Examine the four proteins (A) to (D)

below. Which is more likely to be derived from the
Ramachandran plot? (Hint: 哪一個較
fit 右圖)
(A)

(B)

(C) (D)

12 Which of the following parts of the IgG molecule are not involved in binding to an antigen?
A) Fab
B) Fc
C) Heavy chain
D) Light chain
E) Variable domain

13 Carbon monoxide (CO) is toxic to humans because:

A) it binds to myoglobin and causes it to denature.
B) it is rapidly converted to toxic CO2.
C) it binds to the globin portion of hemoglobin and prevents the binding of O2.
D) it binds to the Fe in hemoglobin and prevents the binding of O2.
E) it binds to the heme portion of hemoglobin and causes heme to unbind from hemoglobin.

14 In hemoglobin, the transition from T state to R state (low to high affinity) is triggered by:
A) Fe2+ binding.
B) heme binding.
C) oxygen binding.
D) subunit association.
E) subunit dissociation.

15 In a conjugated protein, a prosthetic group is:

A) a fibrous region of a globular protein.
B) a nonidentical subunit of a protein with many identical subunits.
C) a part of the protein that is not composed of amino acids.
D) a subunit of an oligomeric protein.
E) synonymous with “protomer.”

16 Which portion of the myosin protein found in muscle is responsible for both ATP and actin
binding?
A) heavy-chain coiled-coiled tails
B) heavy-chain stalk
C) heavy-chain globular head
D) essential light chain
E) regulatory light chain
17 Which of the following only occurs when ATP (not ADP) is bound to the myosin head?
A) actin is released from its binding site on myosin
B) weak binding of myosin head to actin
C) strong binding of myosin head to actin
D) power stroke of muscle contraction
E) none of the above
18 Which of the following is the best description of how actin and myosin work in cooperation to
allow muscle contraction?
A) myosin head groups push against actin filaments to lengthen the sarcomere
B) myosin head groups walk along the actin filament to shorten the sarcomere
C) myosin head groups interact with actin only when the sarcomere is fully lengthened
D) all myosin head groups move along the actin filament simultaneously to shorten the sarcomere

19 Which protein of the thin filament is responsible for calcium binding?

A) tropomyosin
B) troponin complex
C) globular head of myosin
D) calmodulin
E) actin

20 Which of the following proteins is found in a bacterial flagellum?

A) actin
B) myosin
C) flagellin
D) dynein

21 One would expect that defects in the globin genes would be so serious as to cause death in utero.
Which of the following explains how some thalassemias are found in living people?
A) in β-thalassemia, the use of the γ subunit allows individuals to live a normal lifespan
B) individuals who completely lack functional α subunits can live if placed in a hypobaric chamber to
induce hypoxia(低氧)
C) effects are seen when the genes of α chain are nonfunctional.
D) individuals with nonfunctional α chain genes can make β2γ2 tetramers that function normally

22 Which of the following is true regarding the effectors of hemoglobin-oxygen binding?

A) an increase in blood pH will cause hemoglobin to bind more tightly to oxygen
B) increased CO2 from increased muscle activity will result in an increase in the R state of
hemoglobin
C) increased Cl- will cause the formation of a salt bridge between two Lys residues, one on an 
subunit, the other on a  subunit
D) the binding site of 2,3-BPG contains several Asp and Glu residues which are repelled by the
similar charge, pushing the two  subunits away from each other
23 When oxygen is bound to myoglobin, the amino acid _____ is complexed to the iron ion of the
heme group while _______ forms a hydrogen bound to the oxygen.
A) cysteine; serine
B) cysteine; histidine
C) serine; cysteine
D) histidine; histidine
E) histidine; cysteine

24 What is the sequence of the following peptide?

A) Ile-Ala-Thr-Val B) Val-Gly-Ser-Ala C) Leu-Gly-Glu-Ala D) Val-Ala-Ser-

Val E) Val-Gly-Gln-Ala

25 What would be the overall charge at pH 6 for the peptide of Q24?

A) +2 B) +1 C) 0 D) -1 E) -2

26 Which of the following bond does not allow for rotation?

A) 1 B) 2 C) 3 D) 4 E) 5
27 Which is incorrect about antibody molecule ? (A) antibody is made from two heavy chains and
two light chains (B) All chains are held together by disulfide bonds (C) VH domains are the same in all
antibodies (D) CL domains are the same in all antibodies

28 Which amino acid derivative is a neurotransmitter in the video “Matter over mind” that you
watched in the Biochemistry class? (A)Arg (B) Tyr (C) Pro (D) Gly

29

Collagen is composed of triple-helical

tropocollagen cross linking，Refer to the
graph and indicate the amino acid residue
for the linkage between tropocollagen？(A) Glu (B) Gly (C) Lys (D) Arg。