Liofilchem®

Certificate of Analysis Page 1 of 1

Product Batch Expiration date

Tryptic Soy Agar 060418504 2022.05.27

Ref. 610052 – 620052 – 6100525

Physical quality control Specification Results

Expected pH-value (25°C) 7.3 ± 0.2 7.2
Appearance of powder Free-flowing, homogeneous Conforms
Colour of powder Light beige Conforms
Appearance of prepared medium Slightly opalescent Conforms
Colour of prepared medium Light amber Conforms

Microbiological Performance
Tested according to CLSI M22-A3, EN ISO 11133
Reference media: Tryptic Soy Agar – batch already validated

Productivity, Method of control: Quantitative

Inoculum: 50-100 CFU
Control strains Incubation Expected Specification Results
Results
Bacillus cereus Aerobic, 24-48 h PR ≥ 0.7 Good growth Conforms
WDCM 00001 30 ± 1°C
Bacillus subtilis Aerobic, 72 ± 3 h PR ≥ 0.7 Good growth Conforms
WDCM 00003 30 ± 1°C
Escherichia coli Aerobic, 24 ± 2 h PR ≥ 0.7 Good growth Conforms
WDCM 00012 30 ± 1°C
Listeria monocytogenes 4b Aerobic, 40-48 h PR ≥ 0.7 Good growth Conforms
WDCM 00021 37 ± 1°C
Staphylococcus aureus Aerobic, 24-48 h PR ≥ 0.7 Good growth Conforms
WDCM 00034 37 ± 1°C
Clostridium perfringens Anaerobic, 24-48 h PR ≥ 0.7 Good growth Conforms
WDCM 00007 37 ± 1°C
Pseudomonas aeruginosa Aerobic, 40-48 h PR ≥ 0.7 Good growth Conforms
WDCM 00024 36 ± 2°C
Enterococcus faecalis Aerobic, 40-48 h PR ≥ 0.7 Good growth Conforms
WDCM 00087 36 ± 2°C

Batch Release
Approved
Date 07.06.2018 Signature Quality Control
(D. Vitagliano)
The results reported were obtained at the time of release.

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CoA Ref. 610052 – 620052 – 6100525 Rev. 9 of 10.09.2015