AE series SME-v202

AE series SME Ver. 2.
02
FULL AUTOMATIC CHEMISTRY ANALYZER

AE-1000/3000
Installation Instruction &

Maintenance Service Manual
For safe use of AE-1000/3000, please read this Instruction Manual

well and understand function & operation.
Keep this Instruction Manual with you to refer anytime necessary.
AE series SME Ver. 2.02
FULL AUTOMATIC CHEMISTRY ANALYZER
AE-1000/3000
Installation Instruction & Maintenance Service Manual
Contents
0 ELECTRICAL FEATURES AND LINE DISTURBANCE ................................... 1
1 GENERAL DESCRIPTION ..................................................................................... 2

1.1 Introduction ..................................................................................................... 2
1.2 Technical Features ......................................................................................... 2
1.3 Cable Drawing ................................................................................................. 2
1.4 Block Diagram................................................................................................. 3
1.5 Mechanical Device .......................................................................................... 4
1.5.1 Serum Plate .......................................................................................................... 4
1.5.2 Reagent Plate ....................................................................................................... 5
1.5.3 Reaction Plate ...................................................................................................... 6
1.5.4 Reaction & Sample Plate (Double Plate) ...................................................... 7
1.5.5 Reagent & Sample Arm ..................................................................................... 8
1.5.6 Mixer Arm .............................................................................................................. 9
1.5.7 Washing Station ................................................................................................ 10
1.6 Liquid Circuit................................................................................................. 11
１．７ Electrical Circuits ................................................................................... 13
1.7.1 Main Board .......................................................................................................... 13
1.7.2 Motor Driver Board ........................................................................................... 14
1.7.3 Amplifier Board.................................................................................................. 14
1.7.4 Liquid Control Board........................................................................................ 14
1.7.5 Level Sensor Board .......................................................................................... 15
1.7.6 Bridge Board ...................................................................................................... 17
2 ADJUSTMENT OF MECHANICAL GROUP......................................................... 18

2.1 Service Menu................................................................................................. 18
2.1.1 Adjustment ............................................................................................. 18
2.1.2 Open Address ......................................................................................... 20
2.2 Machine Config.............................................................................................. 22
2.3 Base Adjust ................................................................................................... 24
2.4 Adjustment .................................................................................................... 28
2.4.1 Sample Arm (AE-3000)..................................................................................... 28
2.4.2 Reagent Arm ....................................................................................................... 30
2.4.3 Wash Arm ............................................................................................................ 33
2.4.4 Sample Mix Arm (AE-3000) ............................................................................. 34
2.4.5 Reagent and Sample Tray (*When adjustment of Reagent/Sample Arm
is insufficient) .................................................................................................... 35
3 PARTS TESTING ................................................................................................. 36
4 MAINTENANCE.................................................................................................... 37
4.1 Maintenance operation ................................................................................. 38
c.1
4.1.1 Daily Maintenance............................................................................................. 38
4.1.2 Monthly Maintenance ....................................................................................... 38
4.1.3 Six Month Maintenance ................................................................................... 38
4.1.4 One Year Maintenance..................................................................................... 38
4.1.5 Cuvette Maintenance ....................................................................................... 38
4.2 Lamp Replacement ....................................................................................... 39
4.3 Probe Replacement ...................................................................................... 39
5 SHUTTING DOWN FOR A LONG TIME .............................................................. 40
6 TROUBLE SHOOTING ......................................................................................... 40

6.1 Movement Errors .......................................................................................... 40
6.2 Temperature Errors ...................................................................................... 40
6.3 Freeze Error................................................................................................... 41
6.4 Communication Error ................................................................................... 41
6.5 Misadjustment for Mechanical Parts........................................................... 41
6.6 Water Level, Waste Level Error during Testing ......................................... 41
6.7 Serum and Reagent Level Error .................................................................. 41
6.8 Level Sensor Error........................................................................................ 42
6.9 Precision Problem on End Point Analysis ................................................. 42
6.10 Precision Problem on Kinetics Analysis .................................................. 43
6.11 Precision Problem on Fixed Time Analysis.............................................. 44
c.2
0 ELECTRICAL FEATURES AND LINE DISTURBANCE

Disturbances on the power line may be caused by several factors such as:
- Starting / Stopping of electric motors.
- Atmospheric phenomena.
- Connection and disconnection of high line loads.
Serious power supply line disturbances have negative effects on the system.
An efficient ground connection is necessary to reduce line disturbances and guarantee the
maximum safety to the operator.
Power Supply: 220V AC ± 10% 50/60 Hz

Distribution: 1 phase, neutral and ground
Line Fuse: 2 x10A
Symbol Table
If treated mistakenly, a danger to cause death or wound

DANGER is expected to be imminent.
If treated mistakenly, a possibility to cause death or
WARNING wound is expected to be imminent.
If treated mistakenly, a danger to cause wound and
CAUTION physical damage is expected to happen.
Prohibited Action
Necessary Action before using
No warranty applied if you
instrument.
do this action.
There is a possibility to cause disasters by any harmful creature

(especially microorganism / bacterium / virus) leaking to the
environment. And also it may cause ecological accidents which expose
human and natural environment to danger.
1
1 GENERAL DESCRIPTION
1.1 Introduction
ERMA AE-1000/3000 is designed as a full automatic chemistry analyzer system. It includes

Kinetic, Two-Point and End-Point determinations with both Linear and Non-Linear calculation
modes. The instrument is a direct reading Random Access Analyzer supported by an intelligent
multitasking software system.
1.2 Technical Features
AE-1000 AE-3000
Assay Method End Point, Fixed Time, Linearity & Non-Linearity Analysis, Kinetics
Speed 100 tests/hour 200 tests/hour
2 reagents 2 reagents
Assay Item Max 45 items simultaneously
Edit Item Any item, no forbidden
Sample Positions 49 60
Reagent Positions R1=28, R2=28 45
Reagent Bottle 50ml and 23ml 70ml and 20ml
Sample Volume 3-50ul 3-100ul
Reagent Volume R1=25-350ul R1=1-350ul
R2=10-150ul R2=0-200ul
Reagent Minimal
180ul 180ul
Volume
Calibration Linearity/Non-Linearity calibration
Control Add control at any time. Six kinds of control at most for one item
Filter(nm) 340, 405,450, 510, 546, 578, 630 ,700
Light Source 12V 35W halogen lamp
Acceptor 8 pcs imported
Linearity Range 0 - 3.0000Abs.
Temperature Control Cuvette 37±0.2 ℃
Cuvette 120 high quality cuvettes
Washing System 4 nozzles 7 nozzles
Dimensions (mm) 740(W)x570(D)x480(H) 840(W)x740(D)x500(H)
Weight (kgs) 46 70
1.3 Cable Drawing

D-sub 9-pin Crossover Cable must be used to connect PC and the Instrument.
DB 9 for PC DB 9 for Instrument
2 2
3 3
5 5
2
1.4 Block Diagram
The following figure shows a summarized scheme of functional parts of the Instrument:
3
1.5 Mechanical Device i
All the mechanical devices are identified in the following table which provides brief descriptions of
their functions.
1.5.1 Serum Plate
Location Description
1 Center Axis
2 Encoder
3 Photo Micro Sensor
4 Sample Cup
5 Sample Plate Holder
6 Shell of Sample Plate
7 Sample Holder Lower Plate
8 Mechanical Floor for Sample Plate
9 Gear for Sample Plate
10 Motor
4
1.5.2 Reagent Plate
1 Center Axis
2 Encoder
4 Reagent Bottle
5 Reagent Plate Holder
6 Shell of Reagent Plate
7 Reagent Holder Lower Plate
8 Mechanical Floor for Reagent Plate
9 Gear for Reagent Plate
10 Motor
5
1.5.3 Reaction Plate
1 Center Axis
2 Gear for Reaction Plate
3 Shell of Reaction Plate
4 Reaction Plate Holder
5 Hole for Filter Holder
6 Main Body for Reaction Plate (Heating System inside)
7 Mechanical Floor for Reaction Plate
6
1.5.4 Reaction & Sample Plate (Double Plate)
1 Center Axis
2 Encoder
4 Reagent Plate Holder
5 Reagent Bottle
6 Serum Tube
7 Shell for Reagent & Sample Double Plate
8 Reagent Holder Lower Plate
9 Sample Holder Lower Plate
10 Gear for Sample Plate
11 Motor
7
1.5.5 Reagent & Sample Arm
1 Motor for Probe Arm Up and Down
2 Motor for Probe Arm Left and Right
3 Gear for Motor(Left and Right)
4 Photo Micro Sensor for Arm Left and Right
5 Linear Bearing for Arm Up and Down
6 Arm Body
7 Beam for Arm
8 Probe
9 Probe Holder
10 Big Gear for Arm Left and Right
11 Bearing Holder Up
12 Belt for Arm Up and Down
13 Gear for Motor (Up and Down)
14 Bearing Holder Down
15 Washing Unit
16 Holder for Washing Unit
8
1.5.6 Mixer Arm
1 Motor for Mixer Arm Up and Down
2 Motor for Mixer Arm Left and Right
3 Gear for Motor(Left and Right)
4 Photo Micro Sensor for Arm Left and Right
5 Linear Bearing for Arm Up and Down
6 Arm Body
7 Beam for Arm (Mixer Motor Holder)
8 Mixer Holder
9 Mixer Stick
10 Big Gear for Arm Left and Right
11 Bearing Holder Up
13 Gear for Motor(Up and Down)
14 Bearing Holder Down
9
1.5.7 Washing Station
1 Motor for Washing Arm Up and Down
2 Linear Guide for Washing Arm Left and Right
3 Belt Holder
4 Arm Body
5 Washing Nozzle Holder
6 Beam for Arm
7 Washing Nozzle
8 Fixed Screw Up
9 Fixed Screw Down
10 Gear Guide
12 Gear for Motor (Up and Down)
10
AE-1000 Whole Piping Diagram (Ver2.02)
1.5*3.0(Teflon)
Nozzle 3*6 1.4*3.5

AE-1000
XB2 ４ 3*6
1.4*3.5
Diaphragm pump 1.4*3.5
(Type B 600-650ml)
1.6 Liquid Circuit
Nozzle cleaning unit
1.4*3.5 1.4*3.5
Wash station
7*10 3*6 3*6 1.4*3.5
7*10 Syringe
Internal tank (Waste)
Check valve
Solenoid valve
3*6
XB1 ３
11
Transit Block
Diaphragm pump 3*6
(Type B 600-650ml) XA2
3*6
3*6 3*6 3*6 ２Ｃ
１ XA1 Diaphragm pump External joint

７ (Type B 600-650ml)
Diaphragm pump
(Type A 300-400ml)
Solenoid valve
4*7 4*7 4*7 4*7
XB3
3*6
WASTE WATER
3*6 ５Ｒ
Diaphragm pump
(Type B 600-650ml)
AE-3000 Whole Piping Diagram (Ver2.02)
1.4*3.5
AE-3000
Nozzle 1.5*3.0(Teflon) 1.4*3.5
1.4*3.5
Sample
Reagent Nozzle cleaning unit 1.4*3.5 1.4*3.5 1.4*3.5 1.4*3.5
Mixing
Sample
&
7*10 Reagent
Solenoid valve Syringe
Wash station
Internal tank (Waste)
Transit Block
12
3*6 3*6 １ XA1
Diaphragm pump
(Type A 600-650ml)
3*6 3*6 3*6 3*6
3*6
Diaphragm pump
(Type A 600-650ml)
External joint
3*6 3*6 ２
７
XB2 XA2
３ XB1 3*6 Solenoid valve
Diaphragm pump ４
(Type B 600-650ml)
Diaphragm pump XB3 ５
3*6
(Type B 600-650ml)
3*6 Diaphragm pump
(Type B 600-650ml) 4*7 4*7 4*7 4*7
3*6
XB4
3*6
WASTE WATER
６Ｒ
3*6
Diaphragm pump
(Type B 600-650ml)
7*10
１．7 Electrical Circuits
1.7.1 Main Board
The Main Board is the brain of the instrument. All electronic parts are linked to and controlled by
the Main Board.
The Main Board receives commands from external computer. IAP microprocessor takes control of
all actions in the machine. Also, the program for this board is stored inside.
All the movement of mechanical parts is driven by step motor unit. The microprocessor drives 8
stepper motor control and 8 OPB of the mechanical parts.
The microprocessor drives amplifier board in the optical unit. The resolution of the amplifier is 16
bit.
The Main Board is driven by three power supports: +12V, -12V and 27V.
From Thermistor
From Relay board
J8 J1 From
Switching supply
+12V
G12V
Wavelength sensor adjustment -12V
12V Adjust 24V Adjust
+27V
８５４１
G27V
７６３２
12V 24V 5V 5V
JT7 JT6 JT4 JT2
JT7 JT5 JT3 JT1

PC RS232C For Heater
For Liquid sensor
For Pump Board

Reagent Nozzle Sample Nozzle Reaction Tray Reagent Tray Mixing Arm Syringe
The voltage adjustment below applies to AE-1000/3000.

W/L 700 630 578 546 510 450 405 340
Check
J8 J7 J6 J5 J4 J3 J2 J1
Point
Voltage 0 Above 0.3V
heck Point JT-8 JT-7 JT-6 JT-5 JT-4 JT-3 JT-2 JT-1
Voltage 1 4.0V±0.2V
Voltage 0 Confirm the voltage.

Voltage 1 With reaction cell removed, adjust to the voltage above with the VR.1-8 the adjustment.
13
1.7.2 Motor Driver Board
The Motor Driver Board is responsible for driver

step motors. The Main Board has total 8 pcs of
Motor Driver Board, all identical with one
another.
No need for adjustment.
1.7.3 Amplifier Board
The Amplifier Board is responsible for detecting the light

signal from Reaction Liquid and amplifying it. The
Instrument contains 8 pcs of the same board receiving
different wavelengths from 340nm to 700nm.
The Amplifier Board detects the signal, which is sent to the
Main Board and then converted to Abs.
The Main Board supports 8 trimmers to adjust gain and
offset of amplifiers.
1.7.4 Liquid Control Board
The Board controls Vacuum Pump and one Electro

Valve. It is controlled by microprocessor of Main
Board.
14
1.7.5 Level Sensor Board
The Level Sensor Board functions with probe capacitance sensor.

The capacitance changes when the tip of probe touches the liquid. This change is measured by
microprocessor on the Sensor Board and the differential result is conveyed to the microprocessor of
Main Board.
Adjustment of Liquid Level Sensor:

Liquid level sensor is sensitive to the environment (humidity, temperature, ground etc.). Level
Sensor needs to be adjusted when the probe does not run normally due to level sensing problem.
Adjust the volume on the Level Sensor Board by the following procedures:
1. Usually the green LED is on. Turn the volume anticlockwise until the LED goes out.
2. After the LED is out, set a finger to the position approx. 10mm from the tip of the probe and turn
the volume clockwise until the LED is on. After the LED is on, turn clockwise about 3 more times
(attention – the probe will be unable to detect the liquid level if the volume is turned too much).
If adjusted properly, the LED will go out for a moment when the tip of probe reaches liquid level.
For Sample probe (New Type)
LED
1. Confirm the current level of sensitivity. Short-circuit the blue-arrowed pins in the diagram
below for a moment. As LED on the board starts to blink on and off, count the number of
times it blinks. The number of the blinks directly shows the current level of sensitivity.
Front Back
15
2. When increasing the level (decrease sensitivity), the following procedure must be followed.
Short-circuit the blue-arrowed pins in the diagram below for a moment. As soon as the LED
on the board starts to blink, short-circuit the yellow-arrowed pins. Keep the shorted status
while LED continues to blink. This procedure lowers one senstivity level.
Front Back
3. When decreasing the level (increasing sensitivity), the following procedure must be followed.
while LED continues to blink. This procedure raises one sensitivity level.
Front Back
4. After adjusting the level, go back to the procedure (1) to confirm the changed sensitivity.
For Reagent probe (New Type)
LED
1. Confirm the current level of sensitivity. Short-circuit the blue-arrowed pins in the diagram
below for a moment. As LED on the board starts to blink on and off, count the number of
times it blinks. The number of the blinks directly shows the current level of sensitivity.
Front Back
16
2. When increasing the level (deacreasing sensitivity), the following procedure must be followed.
while LED continues to blink. This procedure lowers one senstivity level.
Front Back
3. When decreasing the level (inceasing sensitivity), the following procedure must be followed.
while LED continues to blink. This procedure raises one senstivity level.
Front Back
4. After adjusting the level, go back to the procedure (1) to confirm the changed sensitivity.
1.7.6 Bridge Board
The Bridge Board relays signals from the

Main Board to drive motors and solenoid
valves. The Board also relays signals
from each Photo Micro Sensor to the Main
Board.
17
2 ADJUSTMENT OF MECHANICAL GROUP

The Service Menu allows setting all adjustments for instrument.
2.1 Service Menu

2.1.1 Adjustment
Open the Service Menu and select Adjustment in Service and Adjust menu.
After selecting it the password box becomes active.
The password is 666666. After entering it the service menu will be displayed as follows:
Adjustment differs by model (depending on the number of Arms). Refer to 2-4 for detail.
18
FIELD/BUTTON ACTION DESCRIPTION

Button Press this button for Initialization.
Button Press this button to enter Configuration

screen.
Button Press this button to enter Base Adjust screen.
Field Adjust Sample Arm with the buttons.

Button
Field Adjust Reagent Arm with the buttons.

Button
Field Adjust Sample Mix Arm with the buttons.

Button
Field Adjust Wash Arm with the button.

Button
Field Adjust Reagent/Sample Plate with the

Button buttons.
Button Activity buttons used for adjustment.
19
2.1.2 Open Address
When double click the upper right corner, the Open Adressing window appears.
Double click

Column Input Adress code and read the current
setting value by clicking ‘’In’’.
Column Input Data and change the setting value

by clicking ‘’Out’’
Adress Code Applicable models DESCRIPTION

Reagent Syringe Steps
177 AE1000/3000
AE1000:54 AE3000:27
Sample Syringe Steps
178 AE3000
210(New) 108(Old)
20
Adress Code Applicable models DESCRIPTION

Correction value for sample suction volume
It is used when correcting small suction volume.
184 AE1000/3000 e.g.：If ‘’150’’ is input, Sample Suction Volume plus
1.5ul is suctioned.
0 (AE1000/3000)
Mixing time
185 AE3000
140
Suction time at washing
186 AE1000/3000
80 (AE1000/3000)
Washing time of mixing arm
187 AE3000
140
Washing time of R1 nozzle
188 AE1000/3000
AE1000：150 AE3000：180
Washing time of R2 nozzle
189 AE1000/3000
AE1000：150 AE3000：180
Washing time of sample nozzle
190 AE1000/3000
AE1000：150 AE3000：180
The number of mixing
191 AE1000 It is used to set the number of mixing operation.
1(New)
Hight of mixing
192 AE1000 135(New)
Cell to be washed at measurement
193 AE1000/3000 0(New)
The number of washing time at initialization
197 AE1000/3000 0(New)
Water volume for washing
198 AE1000/3000 135(New)
200 AE1000/3000 340nm Offset setting Default:128(New)
21
2.2 Machine Config
Open the Machine Config screen:
CAUTION
This menu is very important for instrument. All parameters are
stored in the main board. The engineer can backup and restore
the parameters from this menu. Correct settings will help the
machine to work in the right way.
When installing the instrument in hospital for the first time, be sure
to backup the parameters in this menu with the connecting PC.
Do not modify the settings unless instructed by ERMA.
22

Field Temperature Display area. In this menu
the temperature for reaction plate is
displayed in real time.
Model No. Column The model number of the machine. It
cannot be modified.
AE-1000: 100201
AE-3000: 300301
340 Position Column The position of the 340nm filter.
After initialization the position of cuvette
for 340nm filter is displayed.
Washing Position Column The position of the washing probe.
After initialization the position of cuvette
for the first washing probe of the Washing
Station is displayed.
Cleaning Pin Counts Column The number of washing nozzles for
washing station.
AE-1000: 3
AE-3000: 5
Coefficient Column The light path length of the instrument.
The default is 5.
Adjust Cuvette No. Column The number of interspatial cuvettes
between the sample pipetting position
and the reagent pipetting position on the
reaction plate (see 2.4 figure)
AE-1000: 14
AE-3000: 11
Reaction Tray Adjustment Column The correction value for the motor driving
the reaction plate.
Washing Cycle Column One cycle of the cuvette washing is the
time it takes to bring down the washing
nozzles and up again to the initial
position. (e.g. ‘60’ equals 6 seconds in
this setting)
Injection Time Column The level of water supply from the
water-feeding pipe of Washing Unit to the
reaction cells, indicated by coefficient.
Pumping Time Column The level of aspiration of water from the
reaction cells into the aspiration pipe of
Washing Station, indicated by coefficient.
Single Cycle Column The cycle time for Testing with single
reagent mode.
AE-1000: 24s
AE-3000: 15s
*There are some differences among
individual units.
Double Cycle Column The cycle time for Testing with double
reagent mode.
AE-1000: 33s
AE-3000: 19s
*There are some differences among
individual units.
23
Button After setting all parameters in config
menu, press this button to Send the
parameters to Microprocessor of Main
Board.
Button If the parameters are accidentally cleared
or missed by special condition, press this
button to Get parameter from
Microprocessor of Main Board.
Button After all settings and adjustments, press
this button to Backup all the parameters
and settings to PC file.
Button If the parameters and adjustments are
accidentally cleared or missed by special
condition, press this button to Restore all
the parameters and adjustments from PC
file.
2.3 Base Adjust
Press Base Adjust button to enter the menu.
24
This menu allows adjustment of the optical unit.

Field In this area all the information on the base
of the line for each cuvette is displayed.
Usually the base line (Red Line) must be
in the middle of the cuvette line (Blue
Line). See the image below.
Button When the light base is not in the middle of

the cuvette line, there are two conditions.
First: The Red Line is far from the middle

of the cuvette line (Blue Line).
Second: The Red Line is close to the

middle of the cuvette line but has a minor
deviation.
For the first condition, the Optical

(Amplifier) Board on the reaction plate
must be adjusted.
For the second condition, use the adjust

function as shown on the left. Default line
base value is 20. Click +1 or -1 button to
increase or decrease the line base to
adjust the Red Line to the center of the
cuvette line (Blue Line).
After adjusting + / - steps for line base,
validate the adjustment by Adjust button.
Caution: The range of adjustment for

light base is from 18 to 22.
Drop-down Select the wavelength to check light base.

list box
Button Click this button to get light base with
instrument. The reaction plate will turn
around 2 cycles. The light base for the
selected wavelength will be displayed in
the line base area with all of the cuvettes.
25
Button After getting base for the corresponding
filter, click these two buttons to check all
line bases for each cuvette page by page.
Field In this area, one out of 12 pages of the

graph shown on the left side of the screen
(for 10 cuvettes) is divided into 500 equal
parts, each of which A/D value is
displayed.
26
How to adjust the line base?
27
2.4 Adjustment
Horiz ontal Le ve l
Mixe r Arm
Re action Plate
Re age nt Arm
Re age nt Plate
108
1
12
Sample Arm
Sample Plate
Horiz ontal Le ve l
2.4.1 Sample Arm (AE-3000)
28
Press Place 1button for Horizontal position.. Confirm that Sample Arm is right above the
Washing Unit. If it is not centered, slightly loosen the screw (see the photo below) to adjust the
Arm to the center. Press OK button after adjustment. +/- Step buttons will not be used.
DIRECTION PLACE DESCRIPTION

The initialization position for Sample Probe. The correct
Place 1 position is where the sample probe stops at the top
center of Washing Unit.
The correct position is where the sample probe stops at
Horizontal Place 2 the top center of No.1 Serum Cup.
The correct position is where the sample probe stops at
Place 3 the top center of No.12 Cuvette.
Vertical Place 1
29
Place 2 Serum Cup
Place 3
Use +1, -1, +10, -10 step buttons to move the Arm for adjustment. After finishing adjustment,
click OK button to save the adjusted position or click Cancel button to cancel current
adjustment.
2.4.2 Reagent Arm
30
Press Place 1 button for Horizontal position.. Confirm that Reagent Arm is right above the
Washing Unit. If it is not centered, slightly loosen the screw (see the photo below) to adjust the
Arm to the center. Press OK button after adjustment. +/- Step buttons will not be used.

The initialization position for Reagent Probe. The correct
Place 1 position is where the reagent probe stops at the top
center of Washing Unit.
The correct position is where the reagent probe stops at
Place 2 the top center of No.1 reagent bottle.
The correct position is where the reagent probe stops at
Horizontal Place 3 the top center of No.1 Cuvette.
Extended Position for AE-1000. The correct position is
Place 4 where the reagent probe stops at the top center of No.1
Sample Cup.
Extended Position for AE-1000. The correct position is
Place 5 where the reagent probe stops at the top center of
No.29 reagent bottle.
Vertical Place 1
31
Place 2
Vertical Place 3
Place 4 Serum Cup
Place 5 Extended Position for AE-1000. Same as Place 2

Use +1, -1, +10, -10 step buttons to move the Arm for adjustment. After finishing
adjustment, click OK button to save the adjusted position or click Cancel button to cancel
current adjustment.
32
2.4.3 Wash Arm
2mm
Washing
Station
Vertical Place 1 Reaction cuvette
current adjustment.
33
2.4.4 Sample Mix Arm (AE-3000)
Press Place 2 button for Horizontal position. Confirm that Sample Mix Arm is right above the Washing
Unit. If it is not centered, slightly loosen the screw (see the photo below) to adjust the Arm to the
center. Press OK button after adjustment. +/- Step buttons will not be used.

The correct position is where the mixer stick stops at the
Place 1 top center of No.108 Cuvette.
Horizontal The initialization position for Sample Mix Arm. The
Place 2 correct position is the Mixer Stick stops at the top center
of the Washing Unit.
Vertical Place 1
34
Place 2
current adjustment.
2.4.5 Reagent and Sample Tray (*When adjustment of Reagent/Sample Arm is insufficient)
PLATE DESCRIPTION
Reagent Tray1 When pressing the button, Reagent Arm moves to the top
center of No.1 Reagent Bottle (the position adjusted in
2-4-2 Horizontal, Place 2). If the position is slightly off the
center of the bottle, adjust the position of Reagent Tray 1.
Sample Tray1 (AE-3000) When pressing the botton, Sample Arm moves to the top
center of No.1 Sample Cup (the position adjusted in 2-4-1
Horizontal, Place 2). If the position is slightly off the center
of the bottle, adjust the position of Sample Tray 1.
Reagent Tray2 (AE-1000) When pressing the botton, Reagent Arm moves to the top
center of No.29 Reagent Bottle (the position adjusted in
2-4-2 Horizontal, Place 5). If the position is slightly off the
center of the bottle, adjust the position of Reagent Tray 2.
Use +1, -1, +10, -10 step buttons to move the Arm for adjustment. After finishing adjustment,
click OK button to save the adjusted position or click Cancel button to cancel current
adjustment.
35
3 PARTS TESTING
This menu is used for testing the mechanical parts.

Reagent Syringe Field In the blank beside each syringe name, input
the reagent volume that the syringe will
Sample Syringe
aspirate. Then click executing button. Syringe
begins to run and aspirate the solutions on
request. Then click Reset to reset it to the
initial position. If the syringe cannot run or the
volume is incorrect, the syringe encounters
malfunction.
Temperature Field Click Get Temperature button to view the
indicated temperature. If it is different from the
actual cell temperature, input the number of
the difference and click Adjust to adjust the
temperature.
Reagent Height Field Input reagent position in the No. box and click
check mark. Probe runs to check the liquid
36
level of reagent. Pulse number of the
remaining reagent level will be indicated In the
box below. The color of the box (background)
will be displayed in yellow if the remaining
level is low, and in red if there is no remaining
reagent.
Sample Height Field Input sample position in the No. box and click
check mark. Probe runs to check the liquid
level of sample. Pulse number of the
remaining sample level will be indicated in the
box below. The color of the box (background)
will be displayed in yellow if the remaining
level is low, and in red if there is no remaining
sample.
A/D Reading Field Before checking A/D reading, remove reaction
cells 61 – 120 while the power is off. Turn on
the power and let it idle for about 30 minutes.
Then go to Parts Testing menu and click the
check button for A/D reading. A/D value will
be displayed next to each wavelength.
Confirm that the values are around 50,000
with the condition that all the cells are
removed. Click the buttons in order of Set ‘0’,
Save ‘0’. Finish A/D reading by once again
clicking the check button.
If the values are below 20,000, the lamp must
be replaced.
4 MAINTENANCE
37
4.1 Maintenance operation
Professional maintenance will protect the machine and let it work well.
4.1.1 Daily Maintenance
1. Check Distilled-water bottle and empty the waste bottle.

2. Check the dispensing probe position and keep the tip clean.
3. Check if any blockage happened in the washing in-way.
4.1.2 Monthly Maintenance
1. Wash the dispensing probe by detergent solutions.

2. Clean the surface of the instrument.
3. Wash reagent containers.
4.1.3 Six Month Maintenance
Replace all tubes for Roller Pump.
1. Check lamp. If abnormal, replace with a new one.

2. Check if water pumps run normally.
3. Check if there is any leakage in the syringe. If necessary, replace with a new one.
4.1.4 One Year Maintenance
1. Check the power voltage and ground connection.

2. Maintain the mechanical arm.
3. Replace the tubes.
4. Adjust the mechanical positions.
4.1.5 Cuvette Maintenance
1. Configure 0.525% hypochlorous Na solutions.

2. Prepare three bottles for washing the cuvettes. No.1 contains antisepsis solutions; the
other two contain distilled water.
3. Put the solutions in cuvettes into the antisepsis cells.
4. Rinse the cuvettes at least three times with water.
5. Put the cuvettes into No.1 bottle. Immerse them in the water for 10-30mins or a little
longer.
6. Then rinse the cuvettes at least three times.
7. Put the cuvettes into No.2 bottle (distilled water). Immerse them in the water for
10-30mins or a little longer.
8. After immersing, repeat the action of taking out and putting in at least three times in the
distilled water.
9. Put the cuvettes into No.3 bottle (distilled water). Immerse them in the water for
10-30mins or a little longer.
10. After immersing, repeat the action of taking out and putting in at least three times in the
distilled water.
11. After washing, drain off the water in the cuvettes. Put the cuvettes on absorbent material
or holders with mouth down until dried naturally.
12. Heating method is forbidden. It will influence the quality of cuvettes.
CAUTION
38
The cuvettes must be checked and maintained after more than

50 tests. In addition to the instructions above, be sure to confirm
visually that there is no damage on the cuvettes.
4.2 Lamp Replacement
Normally, lamp should be replaced in six months or after 1,500hrs working.
 Open the back cover.

 Remove the light cover.
 Remove the lamp and replace with a new one. Beware of the connector and fix the lamp.
 Install the light cover.
 Restore the back cover.
CAUTION
The replacement should be executed after the lamp cools.
Otherwise it will hurt the operator. Be sure not to touch the body
of the lamp directly when replacing lamps.
4.3 Probe Replacement
If the probe is broken it must be replaced.
 Remove the cover of the probe.

 Unscrew the fixed screw.
 Replace with a new one.
 Fix the new probe.
 Adjust the probe position in the mechanical adjustment menu.
 Re-cover the probe.
CAUTION
The position adjustment should be executed carefully and
precisely. Otherwise it will damage the probe.
39
5 SHUTTING DOWN FOR A LONG TIME

When shutting down the machine for more than two days,
1. Use new distilled water filling in the tank.
2. Enter “System Maintenance”, wash cuvettes more than two times.
6 TROUBLE SHOOTING
6.1 Movement Errors
Movement errors happen when the software fails to find a reference during a movement.
Possible Causes:
 Something is obstructing the movement
 Movement flag is broken
 Motor is broken or is in a self-safety status
 Motor driver on the Main Board is broken
 Bridge Board is broken
 Motor cable is badly connected
 Cable for Photo Micro Sensor is badly connected
 Photo Micro Sensor is broken or some light noise is present in the environment
 Other mechanical causes
Solutions:
 Check mechanical system
 Check flag
 Check motor
 Check Main Board temperature
 Check driver on Main Board
 Check any light source near Photo Micro Sensor
 Check Bridge Board
 Check cables on Main Board and Bridge Board
 Check cables connecting Photo Micro Sensor to Bridge Board
6.2 Temperature Errors
Possible Causes:
 Heating system is broken
 Cable for heating system is badly connected
 Temperature sensor is broken or is not working properly
 Temperature sensor cable is badly connected
 Heating control of Main Board is broken
Solutions:
 Check Heating System
 Check the cable connection
 Check connection between temperature sensor and heating system
 Check cable between temperature sensor and Main Board
 Replace the board
40
6.3 Freeze Error
Possible Causes:
 Peltier is broken
 Power of freeze system is broken
Solution:
 Check Peltier. If it is broken, replace it.
 Check the power of freeze system. Check the voltage of 12V switching regulator.
6.4 Communication Error
Possible Causes:
 Serial cables are connected to wrong ports or badly connected
 Main Board is broken
 Main Board has no power supply
Solution:
 Check serial cable connections and choose the right ports
 Check Main Board and power supply
6.5 Misadjustment for Mechanical Parts
Possible Causes:
 Instantaneous current or voltage shocks led to this condition.
Solution:
 Restore Adjustment from file with SERVICE MENU- MACHINE CONFIG.
6.6 Water Level, Waste Level Error during Testing
Possible Causes:
 Waste bottle is full
 Water bottle or acid bottle or basic bottle is empty
 Level sensors are broken or not working properly
 Cables between level sensor and connector are badly connected
 Cable between connector and Main Board is badly connected
Solution:
 Check bottle level
 Check level sensor
 Check cables between level sensor and connector
 Check cable between connector and Main Board
6.7 Serum and Reagent Level Error
Possible Causes:
 Some serum tubes and reagent bottles are empty
 Faulty adjustment
 Level sensor on Level Sensor Board is broken
 Level Sensor Board is broken or badly power supplied
Solution:
 Check reagent and serum levels
 Check adjustment
 Check level sensor
 Check Level Sensor Board
41
6.8 Level Sensor Error
Possible Causes:
 Bad ground connection
 Bad connection of cable between the probe and Level Sensor Board
 Level Sensor Board is broken or badly power supplied
Solution:
 Check all ground connection for the instrument.
 Check the cable between the probe and Level Sensor Board.
 Check Level Sensor Board and power support.
6.9 Precision Problem on End Point Analysis
This problem causes bad CV% on end point analysis. Possible causes can be divided in
four groups:
 Preparation Problems
 Reading Problems
 Washing Problems
 Mechanical Problems
Possible Preparation Problems:

 Probe is damaged
 Probe level sensor on serum is not working properly
 Probe is not washed properly
 Probe up-down adjustment on washing unit is not good
 Probe up-down adjustment on reaction cuvette is not good
 Dilutor is damaged
 Motor cable of dilutor is badly connected
 Liquid circuit is full of air bubbles
Solution:
 Check probe
 Check Level Sensor Board and probe level on serum
 Check liquid circuit of washing unit and be sure that water in the bottle is not dirty
 Check probe up-down adjustment on washing unit (probe must be 2-3 mm below the
water level )
 Check probe up-down adjustment on reaction cuvette (probe must be 2 cm below upper
cuvette level, and it must be in the center of the cuvette)
 Check dilutor
 Check motor cable of dilutor and its connection (see Movement Error section)
 Check liquid circuit and all liquid circuit connection under pressure
Possible Reading Problems:

 Amplifier Board is damaged
 There is not enough voltage for some wavelengths
 Base Adjust is not properly done
 Lamp is badly working
 Optical system is defective
 Silicone diodes are dirty
 Filters are dirty
 Environmental noises are present
 Cuvettes are damaged
Solution:
 Check Amplifier Board
 Check voltage for all wavelengths
42
 Check 2.3 Base Adjust
 Check lamp and lamp power supply
 Check optical system at the wavelengths with poor results
 Check silicone diodes
 Check if filters are dirty
 Check if environmental noises are present
 Check cuvettes
Possible Washing Problems:

 Washing solution and water bottles are empty
 Washing solution is expired
 Water inside the bottle is dirty
 Washing station movement is not correct
 Washing station adjustment is not correct
 Washing station probes are damaged
 Washing liquid circuit is damaged or obstructed
 Some pumps are broken
Solution:
 Check washing solution and water level
 Check washing solution
 Check water
 Check washing station mechanical system (the far right Tip must reach the bottom of
cuvette)
 Check washing station movement (see Movement Error section)
 Check washing station adjustment (the far right Tip must fit cuvette)
 Check washing station probes
 Check washing liquid circuit
 Check pumps
Possible Mechanical Problems

 Movement of Reaction Plate is not smooth
 Mixer Stick is not working well
Solution:
 Check movement of Reaction Plate
 Check Mixer Stick
6.10 Precision Problem on Kinetics Analysis
This problem may cause too high/low readings on kinetics analysis; possible causes can
be divided in two groups:
 Temperature Problems

 There is not enough voltage for 340 wavelength
43
 Lenses on the optical system are dirty
 Environmental light noises are present
Solution:
 Check voltage for 340 wavelength
 Check and clean lenses
 Check environmental light
 Check cuvettes
Possible Temperature Problems:

 Reaction temperature is badly calibrated
 The cable of heating system is badly connected
 Temperature sensor is broken or is not properly working
Solution:
 Check heating system
 Check cable of heating system connection
 Check connection between temperature sensor

Solution:
6.11 Precision Problem on Fixed Time Analysis
This problem may cause too high/low readings or bad CV% on Fixed Time analysis;
possible causes can be divided in two groups:
 Temperature Problems
 Preparation Problems
 Washing Problems

 There is not enough voltage for 340 wavelength
44
 Lenses on the optical system are dirty
 Environmental light noises are present
 Method parameters (Start point, End point) are not properly programmed
Solution:
 Check voltage for 340 wavelength
 Check and clean lenses
 Check environmental light
 Check cuvettes
 Check method parameters
Possible Temperature Problems:

 Reaction temperature is badly calibrated
 The cable of heating system is badly connected
 Temperature sensor is broken or is not correctly working
Solution:
 Check heating system
 Check cable of heating system connection
 Check connection between temperature sensor
Possible Preparation Problems:

 Probe is damaged
 Probe level sensor on serum is not working properly
 Probe is not washed properly
 Probe up-down adjustment on washing unit is not good
 Probe up-down adjustment on reaction cuvette is not good
 Dilutor is damaged
 Motor cable of dilutor is badly connected
 Liquid circuit is full of air bubbles
Solution:
 Check probe
 Check Level Sensor Board and probe level on serum
 Check liquid circuit of washing unit and be sure that water in the bottle is not dirty
45
 Check probe up-down adjustment on washing unit (probe must be 2-3 mm below the
water level )
 Check probe up-down adjustment on reaction cuvette (probe must be 2 cm below upper
cuvette level, and it must be in the center of the cuvette)
 Check dilutor
 Check motor cable of dilutor and its connection (see Movement Error section)
 Check liquid circuit and all liquid circuit connection under pressure
Possible Washing Problems:

 Washing solution and water bottles are empty
 Washing solution is expired
 Water inside the bottle is dirty
 Washing station movement is not correct
 Washing station adjustment is not correct
 Washing station probes are damaged
 Washing liquid circuit is damaged or obstructed
 Some pumps are broken
Solution:
 Check washing solution and water level
 Check washing solution
 Check water
 Check washing station mechanical system (the far right Tip must reach the bottom of
cuvette)
 Check washing station movement (see Movement Error section)
 Check washing station adjustment (the far right Tip must fit cuvette)
 Check washing station probes
 Check washing liquid circuit
 Check pumps

Solution:
46
European Regulatory Affairs Only

EMERGO EUROPE
Molenstraat 15
EC REP 2513 BH, The Hague

The Netherlands
Phone:+31.70.345.8570
Fax:+31.70.346.7299
IVD
2-31-6 Yushima, Bunkyo-ku, Tokyo 113-0034; Japan

Phone:81-3-3818-6281
F a x:81-3-3813-7301
E-mail address: trade@erma.co.jp

AE series SME-v202

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AE series SME Ver. 2.

FULL AUTOMATIC CHEMISTRY ANALYZER

Installation Instruction &

For safe use of AE-1000/3000, please read this Instruction Manual

1 GENERAL DESCRIPTION ..................................................................................... 2

2 ADJUSTMENT OF MECHANICAL GROUP......................................................... 18

3 PARTS TESTING ................................................................................................. 36

5 SHUTTING DOWN FOR A LONG TIME .............................................................. 40

6 TROUBLE SHOOTING ......................................................................................... 40

0 ELECTRICAL FEATURES AND LINE DISTURBANCE

Power Supply: 220V AC ± 10% 50/60 Hz

If treated mistakenly, a danger to cause death or wound

There is a possibility to cause disasters by any harmful creature

ERMA AE-1000/3000 is designed as a full automatic chemistry analyzer system. It includes

1.2 Technical Features

1.3 Cable Drawing

DB 9 for PC DB 9 for Instrument

1.5.1 Serum Plate

Nozzle 3*6 1.4*3.5

Nozzle cleaning unit

１ XA1 Diaphragm pump External joint

4*7 4*7 4*7 4*7

Nozzle 1.5*3.0(Teflon) 1.4*3.5

1.7.1 Main Board

JT7 JT5 JT3 JT1

For Liquid sensor

For Pump Board

The voltage adjustment below applies to AE-1000/3000.

Voltage 0 Confirm the voltage.

The Motor Driver Board is responsible for driver

No need for adjustment.

1.7.3 Amplifier Board

The Amplifier Board is responsible for detecting the light

No need for adjustment.

1.7.4 Liquid Control Board

The Board controls Vacuum Pump and one Electro

No need for adjustment.

The Level Sensor Board functions with probe capacitance sensor.

Adjustment of Liquid Level Sensor:

For Sample probe (New Type)

For Reagent probe (New Type)

1.7.6 Bridge Board

The Bridge Board relays signals from the

No need for adjustment.

2 ADJUSTMENT OF MECHANICAL GROUP

2.1 Service Menu

After selecting it the password box becomes active.

FIELD/BUTTON ACTION DESCRIPTION

Button Press this button to enter Configuration

Button Press this button to enter Base Adjust screen.

Field Adjust Sample Arm with the buttons.

Field Adjust Reagent Arm with the buttons.

Field Adjust Sample Mix Arm with the buttons.

Field Adjust Wash Arm with the button.

Field Adjust Reagent/Sample Plate with the

Button Activity buttons used for adjustment.

FIELD/BUTTON ACTION DESCRIPTION

Column Input Data and change the setting value

Adress Code Applicable models DESCRIPTION

Adress Code Applicable models DESCRIPTION

Open the Machine Config screen:

FIELD/BUTTON ACTION DESCRIPTION

2.3 Base Adjust

Press Base Adjust button to enter the menu.

Nozzle 36 1.43.5

47 47 47 47

Nozzle 1.53.0(Teflon) 1.43.5