● Topic Name :- Formulation and Evaluation of herbal anticream from Citrullus lanat

Seed oil
● Prepared by :- Ms. Rani Narayan Mohite
● Guide Name :- Mrs.Varsharani patil
● Prn no. :- 2063813823029
● Class :- Final Year B. Pharm
Content

1. Introduction
2. About the acne
3. Plant profile
4. Drug Profile
5. Experimental work
6. Phytochemicals Screening
7. Formulation of herbal cream
8. Results
Introduction

Now a day's the majority of Indians are affected by the persistent skin
illness known as acne vulgaris. Sebaceous glands are involved in the
chronic inflammatory skin condition known acne. Propionibacterium acnes
(P. acne), altered follicular keratinization, inflammation, and androgen-
induced enhanced sebum hyper- production are four primary
pathophysiology.Due to their minimal adverse effects, many now prefer
using herbal products over synthetic ones. We create the anti-acne face
cream using ingredients like watermelon seed extract also known as Rubia
cordifolia, is found close to streams and rivers.It contains additional qualities
including anti-ageing, anti-inflammatory, antibacterial, etc. and is typically
used to treat acne, Watermelon seeds extract is filled with vitamin E,
minerals contents, amino acids and antioxidants. It advantageous and
mostly used to cure skin conditions. Certain evaluation test are performed to
check whether cream suitable for human skin, its checks whether cream
cause the irritancy after applying to skin or not.
About the acne
Acne vulgaris is a common cutaneous inflammatory disorder of the
pilosebaceous unit, which runs a chronic course. The condition commonly
manifests with papules, pustules, or nodules primarily on the face, although it
can also affect the upper arms, trunk, and back. AThe pathogenesis of acne
vulgaris involves the interaction of multiple factors that ultimately lead to the
formation of its primary lesion, which is known as "comedoAlthough acne
vulgaris is commonly observed among adolescents, it is not restricted to this
age group and can affect individuals of various ages.The severity of this
condition can vary, ranging from a mild presentation with only a few comedones
to more severe forms characterized by disfiguring inflammatory manifestations,
which can lead to hyperpigmentation, scarring, and adverse psychological
effect. Acne refers to a variety of blemishes caused by the blockage of pores by
dead skin cells and oils emerges through a pore.4
Plants Profiles:
Morphological Characteristics: The watermelon seed
(Citrullus lanatus) is a dicotyledonous plant, and its seeds
exhibit certain morphological features.

Here are some key morphological characteristics of

watermelon seeds:

Shape and Size: Watermelon seeds are generally small,

flat, and ovate in shape. The size of the seeds can vary,
but they are typically about 1 to 2 centimeters in length.

Color: The color of watermelon seeds can range from

black to brown, depending on the variety of watermelon.
Surface Texture: The seed coat (outer layer) of
watermelon seeds is smooth and hard. The surface may
have a glossy appearance.
Drug Profile

Fig.1. Watermelon seed

Uses
Here are some potential pharmacological activities associated with watermelon seeds:

Antioxidant Properties: Watermelon seeds contain antioxidants, including polyphenols,

which may help combat oxidative stress in the body. Antioxidants play a role in protecting
cells from damage caused by free radicals.

Anti-Inflammatory Effects: Some studies suggest that watermelon seeds may possess
anti-inflammatory properties. This could be beneficial in managing inflammation- related
conditions.

Cardioprotective Potential: The presence of certain compounds in watermelon seeds, such

as arginine and cucurbitacin E, has been studied for their potential cardiovascular benefits
including blood pressure regulation and improving vascular function.
Experimental Work:
Soxhlet-extraction of the watermelon seed Oil

Weighed quantity of the sun-dried watermelon seeds

were de-husked, dry-milled into fine crumbs with locally
fabricated electrical grinding machine. The seed mill was
sun- dried again till a constant weight was achieved using
an electric drying oven at 45 OC for six hours, to reduce
the water content, before the extraction process.The
soxhlet extraction apparatus were set up on a regulated
heating mantle at 60 OC. 20grams of the seed mill were
wrapped in a porous cellulose thimble, placed in the
extraction chamber containing n-hexane and allowed to
soak for 30 minutes. The mixture of the extracted oil and
solvent were separated after by heating, at a higher
temperature of 60 oc.The solvent was later recovered,
while the oil was left open in the flask for 4 hours, for
further evaporation traces of solvent.

Fig. 2
Phytochemical Screening :-
Sr. No. Test Results

1. Alkaloids +

2. Glycosides +

3. Tannins +

4. Flavonoids +

5. Steroids -

6. Carbohydrate -

Fig. 3
Formulation of Antiacne cream

Sr.No Ingredients Formulation Cream Formulation Procedure:

Heat liquid paraffin and beeswax in a borosilicate
1 Seed 3.5ml glass beaker at 75 °C and maintain that heating
Extract temperature. [oil phase]In another beaker, dissolve
2 Bees wax 3gm borax, methyl paraben in distilled water and heat this
beaker to 75 °C to dissolve borax and methyl paraben
3 Liquid 10ml to get a clear solution. [Aqueous phase].Then slowly
Paraffin add this aqueous phase to heated oily phase.Then
add a measured amount of watermelon seed extract
4 Borax 0.2mg
and stir vigorously until it forms a smooth cream.Put
5 Methyl 0.2mg this cream on the slab and add few drops of distilled
paraben water if necessary andmix the cream in a geometric
manner on the slab to give a smooth texture to
6 Distilled 5 ml thecream and to mix all the ingredients properly.This
water method is called as slab technique or extemporaneous
7 Rose oil 0.1ml method of preparation
Results and Discussion:-
Parameters Formulation

1.Physical a) Visual a) Homogeneity

Appearance Inspection
b) Odour b) Odourless
c) Consistancy c) Smooth
d) State d) Semisolid

2. Irritancy a) Irritant effect a) Nil

b) Erythema b) Nil

3. Wash ability a) Wash ability washable

4. Ph a) Ph meter 5.5

5.Viscosity a) Viscosity 1747

.
● .
Summary

The present work is concerned with formulation of herbal cream using citrullus
lanatus seed oil.

The formulated cream showed the anti-acne activity as per the commerical
standard and all other parameters were good and hence, it can be concluded
that the formulated herbal cream must be standardized and can be used as a
promising alternative to commercial containing anti-acne cream.
Reference
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Saleem, Sadia Zahra Siddiqui, and Viqar Uddin Ahmad, Preparation of new
formulations of anti-acne creams and their efficacy, African Journal of
Pharmacy and Pharmacology Vol. 4(6), pp. 298-303. June 2010.
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causes and amazing remedial measures for acne. Indo American journal of
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acne. Journal of European academy of dermatology and venereology 2001;
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Updated 2023 Feb 16] Treasure Island (FL): StatPearls Publishing; 2023
Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK459173/