Thy rox ine( T4)c irculatesi nthebl ooda sa nequi li

brium mi xtur eoff reea nds erum pr oteinboundhor mone. Thy r

ox i
nebi ndi ng
globul i
n( TBG) ,albumi nandpr ealbumi nbi ndappr oximat ely75%,10%,and15%oft het ot alc i
rcul ati
ngT4r espec t
ivel y.The
bindi ngofT4byt hes epr oteinsi ss uc ht hatlesst han0. 03%i spr es enti nt hec irc ulationasunbound,f reeT4.Thi ss mal l
per cent ageoft het ot al T4repres entst hephy si
ologi call
yav ailablehor monewhi c hi sbi ologi call
yac ti
v e.Onc et hef r
eeT4i s
abs or bedbyt het a rgetc ell
s,theequi l
ibrium rees t
abl i
shesc ir
c ula
tingf reeT4l evels. Theequi l
ibrium r es ult
sint hema i
nt ena nce
ofac ons t
antl ev eloff r eeT4whenal terat i
onsoc c urinei thert hec onc ent r
ationoraf finityoft hes erum bi ndingpr ot eins.
Ther ef ore, i
nav arietyofnor mal (
pregnanc y)andabnor mal (Fami li
al Dy salbumi nemi cHy per thyrox inemi a, FDH)s tates ,oras
ar es ultoft headmi nistrati
onofc ertaindr ugs(e.g. furosemi deandf enc l
ofenac )
, thet argett issuesar eass uredofr ec eiv i
ng
ther equi reda mountofhor mone. FreeT4v al
uesma y,t
heref ore,pr ovidet hebes tindi ca t
ionoft hy roiddy sfunc t
ion, si
nc ef r
ee
T4i sl es ss ens it
iv et oc hangesi nt hes erum bi ndingpr oteins .
Hi storically,thedi agnos isoft hy roidf unc tionhasi nv ol ved
per f
or mi ngat ota lT4a ssayina dditiont oaThy roxineUpt ak e(TU)a ssayoft hes ames ampl e.Thema thema ticalcombi na ti
on
oft hes et woas s ayspr oduc esaF reeThy roxi
neI ndex( FTI)whi chpr ov i
desani ndi rectpr opor tionales timat ef orf reeT4.
Alter nat i
v el
y ,di rectas sayshav ebeendev el
opedus ingequi libri
um di alysis,utr
titation, RI A, ands oli
d-phas eEIAt ec hnol ogy
tomea s uref reeT4. Int hesemet hods ,sepa r
ati
onoff reea ndboundt rac erisa c
hiev edei therwi thamembr ane, orbybi ndi ng
freeT4t oas olidpha sea nti
body .Thi sex tracti
ons tepr emov esa na mountofT4whi chi spr opor tiona l t
ot heor i
gina la mount
off reeT4pr es enti nt hepa t
ient:sampl e. Provi
dedt hattheex tractedT4i slesstha na ppr oxima tely5%oft heT4i nthes ampl e,
at r
uee stima tionoft hef reeT4c ont entc a nbeobt aine d.TheF reeT4( FT4)Re agentKi tistobeus eda sa naidi nt hea sse ssme nt
oft hy roids t atus .

Spec
ifi
cat
ion
C2000

Sampl
ety
pe Ser
um/
Plas
ma

Sampl
evol
. 50uL

As
sayt
ime 36mi
nut
es

Tes
trange 0.
1-5.
0ng/
dL

Per
for
manc
e CV<8%

St
orage 2-8º
C

Shel
fli
fe 18mont
hs

Cal
i
brat
ion 90day
sVal
i
d

Per
for
manc
e

Or
der
ingi
nfor
mat
ion
RefNo. Tes
t/k
it
Fr
eeT4( FT4)
As
sayReagentKit(
CMI
A) C2KB002 30or60