QUICK PROTOCOL

ZymoBIOMICS™
Oral Microbiome Standard
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Printed on 100% post-consumer recycled paper

 Microbial Composition
QUICK PROTOCOL Theoretical Composition (%)
Species
Genomic 16S Genome Cell

1. Thaw the standard on ice. Vortex the standard DNA Copy1 Copy2 Number3

for at least 30 seconds, then spin down briefly. Streptococcus parasanguinis 16 16.9 17.1 17.1

Neisseria subflava 16 15.8 16.1 16.1

Note: Cells might aggregate due to freeze-thaw cycling; Veillonella parvula 16 16.7 17.0 17.0
therefore, it is critical to mix the cellular standard Prevotella nigrescens 16 12.6 12.8 12.8
thoroughly before use.
Haemophilus parainfluenzae 8 12.9 8.8 8.8

2. For DNA extraction of the standard, use 75 µl Streptococcus mitis 8 9.0 9.1 9.1

per prep. The mechanical lysis featured in the Schaalia odontolytica 8 5.6 7.6 7.6
ZymoBIOMICS™ DNA isolation kits are Rothia dentocariosa 8 5.4 7.3 7.3
recommended for unbiased and efficient Fusobacterium nucleatum 1 1.2 1.0 1.0
isolation. Expected yield is approximately Streptococcus mutans 1 1.4 1.2 1.2
500ng of DNA per prep when using the Streptococcus salivarius 1 1.5 1.0 1.0
ZymoBIOMICS™ DNA Miniprep Kit (D4300).
Porphyromonas gingivalis 1 1.0 1.0 1.0