Conversion of lignocelluloses to commodity

and specialty biochemicals by microbial cell

factory and enzymatic process

1G/2G/3G
Valorization of renewable carbons

Verawat Champreda
National Center for Genetic Engineering and Biotechnology
(BIOTEC)
1
 Bio-industry: New S-curve industry for Thailand

BIO-industry is becoming a key Fuels:

new driver for BCG ECONOMY Ethanol (E10/E20/E85),
biodiesel (B5), Adv biofuels
platform in Thailand.
Diverse agricultural products/
by-products Chemicals:
commodity & specialty
Existing industry platform

Materials:
1G 2G 3G Bioplastics (PBS, PLA) &
Non-Edible Algae biomaterials
Edible

Biospeacialties:
Bioactive compounds/ Microbial
functional products
IBBG: Biorefinery and Bioproduct Technology Research Group
Lab to pre-pilot microbial product development

Renewable Biodiversity
resource +
SYN-BIO Mutant
1st ASEAN platform platform BCG industry
7th World
1G Enzyme &
Sugar Biocatalyst
Starch
Oil
Commodity &
2G Specialty
Biochemicals
Cellulosics
Biocontrol
agents
Bio 1 → 300 L
waste Natural products
Food waste & Bioactive
compounds
Glycerol
Biomass-
CO2 derived
products
3
IBBG platform technology : Integrated Bio-/ Chemical Biorefinery
Green biomass fractionation process for agricultural waste valorization
C 2G To cell Biofuels & Biochemicals
Lab & Bench Scale Flow Through sugar factory
Reactor for Biomass fractionation
Cellulose Packaging Hydrogel
composite
Bagasse H Conversion Separation Bioactivity Oligosaccharide
Hydrothermal/Organosolv fractionation & Pre-biotic Functional sugars
Green biomass fractionation technology (TIM gut
Building block
simulation)
Up-scaling prototype process to kg chemicals
Catalysis
Wood chips L
1L 7.5 L 50 L
Techno-economic evaluation
Palm waste
Reactor systems

Fruit waste
Downstream units

E Phytochemicals
IBL Pre-pilot facilities @INC2 4
Bioprocess Chemical process
Technology platform: Synthetic biology cell factory
design
Specialized yeast for bioprocessing of 1 and 2nd Gen biochemicals
st

1G Sugar Glycerol 2G Biomass

Anti-oxidants
Isoprenoids (4)
Functional ingredient

Bioplastic
monomers
D-Lactic acid (4)
Biomaterial

Fragrance
compounds
Agar wood aroma (4)
Cosmetics & Healthcare

Chemical
precursor
Machine learning Citrus isoprenoid (4)
Healthcare & Pharma
technology
Biochemical characterization of auxiliary enzymes for enhancing
biomass saccharification: BG-AA9 LPMO
Engineered production of isobutanol from sugarcane trash
hydrolysates in Pichia pastoris

Glucose xylose

Growth profile of engineered P. pastoris strain

Production in minimal yeast medium containing a mixed
carbon source (1.7% glucose, 0.3% xylose) that mimics
sugarcane bagasse hydrolysates

1.5% Sugar 2% Sugar

Bumrungtham et al. (2022) J Fungi 8, 767 Total isobutanol production of engineered P. pastoris in a sugarcane trash medium.
Socioeconomic analysis of the integrated sugarcane trash utilization process
Indicators Unit Conventional management Alternative management
Open burnt Electricity Biorefinery Biorefinery
generation (scenario 1) (scenario 2)
Global warming kgCO2 90.43 -408.04 6,289 432
potential
- Cumulative energy MJ 31.23 -7,849.89 91,410 -16,551
demand
- Terrestrial kg 1,4- DB 0.07 -0.22 7.925 4.08
ecotoxicity eq
-Human toxicity kg 1,4- DB 24.83 -156.88 2,931 66.10
eq
-Value added THB/ ton 697 154,059 200,706
ST
-Productivity Unit 2,173 793 793
(kWh, kg)
-Employment man-day 0.73 0.633 0.633

• Sugarcane trash utilization both electricity feedstock and high-

value biochemical have positive impacts on socio-economics.
• ST management by bioenergy generation showed the highest
merit in environmental aspect and gain employment rate.
• The biorefinery approach showd the highest advantage in term
Alternative product from sugarcane trash flow chart of economics dimension.
• Both ST utilization are beneficial to support the sustainable
development issue compared with conventional open burnt.
KMUTT-BIOTEC research on carbon neutrality

Integrative Biorefinery Laboratory

Renewable carbon utilization by multi-disciplinary technology for BCG economy

Core research scopes

12 Current collaborators
Chemical Sugar & Agri-processing
Hydrogen society industry
Bio Biofuel & Biorefinery
Renewable C Biorefinery industry
Chemical & plastic industry
Oleochemicals Petrochemical & fuel
Prof.Dr.Navadol
Laosiripojana
industry
Dr.Verawat CO2 capture & utilization Process up-scaling &
Champreda
Director IBBG demonstration
Grant sources
Acknowledgment
Financial support

Academic partners