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CHAPTER 10
BIOTECHNOLOGY

Multiple-Choice Questions E 6. Rice grains containing consequent amounts of

beta-carotene would
IMPACTS, ISSUES: GOLDEN RICE OR a. support growth of children feeding on
FRANKENFOOD Golden Rice.
b. support the development of a strong
E 1. The Golden Rice is a genetically modified rice
immune system.
plant that produces
c. support the development of good vision.
a. alpha-carotene in its seeds.
d. support all of these.
b. vitamin A.
e. support none of these.
c. beta-carotene in its seeds.
Answer: d
d. vitamin C. Bloom’s Taxonomy: Knowledge
e. gamma-carotene in its seeds.
Answer: a M 7. Which of the following nutrients is likely to
Bloom’s Taxonomy: Knowledge have a strong impact on vision?
a. cholesterol
E 2. A genetically modified rice plant is also called
b. vitamin A
a. transgenic rice.
c. vitamin B12
b. cloned rice.
d. vitamin D
c. transformed rice.
e. galactose
d. recombined rice.
Answer: b
e. transduced rice.
Bloom’s Taxonomy: Knowledge/Comprehension
Answer: a
Bloom’s Taxonomy: Knowledge M 8. Which of the following plants makes and stores
beta-carotene in its seeds?
M 3. The Golden Rice was developed by
a. regular rice
a. Beyer.
b. corn
b. Pasteur.
c. wheat
c. Potrykus.
d. peanut
d. Beyer and Pasteur.
e. all of these
e. Beyer and Potrykus.
Answer: b
Answer: e
Bloom’s Taxonomy: Knowledge
Bloom’s Taxonomy: Knowledge
M 4. Genetically modified organisms are engineered FINDING NEEDLES IN HAYSTACKS
a. on wilderness.
E 9. Which of the following molecules are able to
b. on farms.
digest DNA molecules?
c. in laboratories.
a. lipases
d. on wilderness and on farms.
b. proteases
e. on farms and in laboratories.
c. restriction enzymes
Answer: c
d. DNA ligases
Bloom’s Taxonomy: Knowledge
e. DNA helicases
M 5. Using biotechnology, the Golden Rice was Answer: c
engineered in order Bloom’s Taxonomy: Knowledge
a. to increase the yield.
E 10. Restriction enzymes can cut
b. to improve the nutritional value of regular
a. tRNA.
rice.
b. mRNA.
c. to save money.
c. rRNA.
d. to increase the yield and to improve the
d. DNA.
nutritional value of regular rice.
e. mRNA and DNA.
e. to increase the yield, improve the
Answer: d
nutritional value of regular rice, and to Bloom’s Taxonomy: Knowledge
save money.
Answer: b
Bloom’s Taxonomy: Knowledge/Comprehension

Biotechnology 103
 E 16. Many restriction enzymes generate
D 11. Which of the following is NOT true about
__________ on DNA fragments after
restriction enzymes?
digestion.
a. Restriction enzymes recognize specific
a. frank ends
DNA sequences.
b. blunt ends
b. Restriction enzymes cut DNA
c. curved ends
independently of its origin.
d. sticky ends
c. Restriction enzymes recognize specific
e. oblique ends
nucleotide sequences.
Answer: d
d. Restriction enzymes cut RNA sequences at
Bloom’s Taxonomy: Knowledge
specific sites.
e. All of these are NOT true. M 17. During the cloning process, two fragments of
Answer: d DNA are joined together with the help of a
Bloom’s Taxonomy: Knowledge/Comprehension/Analysis a. DNA ligase.
b. DNA recombinase.
M 12. Which of the following discoveries allowed
c. DNA flipase.
researchers to cut huge DNA molecules into
d. DNA clonase.
manageable fragments?
e. DNA connectase.
a. restriction enzymes
Answer: d
b. reverse enzymes
Bloom’s Taxonomy: Knowledge
c. polymeric enzymes
d. cloning enzymes D 18. Which of the following lists contains a
e. recombination enzymes combination of molecules involved in the
Answer: a cloning process?
Bloom’s Taxonomy: Knowledge a. DNA fragments, DNA ligase, and plasmid
b. DNA ligase, DNA fragments, and DNA
D 13. __________ is an example of a __________.
recombinase
a. DNA recombinase; digestive enzyme
c. DNA fragments, DNA ligase, and plastid
b. DNA ligase; digestive enzyme
d. DNA fragments, DNA connectase, and
c. EcoRI; restriction enzyme
plastid
d. E. coli; restriction enzyme
e. DNA ligase, DNA fragments, plastid, and
e. mRNA; cloning enzyme
DNA recombinase
Answer: c
Bloom’s Taxonomy: Knowledge/Comprehension Answer: d
Bloom’s Taxonomy: Knowledge/Comprehension/Analysis
D 14. Which of the following sequences is
D 19. Which of the following describes most
recognized by the restriction enzyme EcoRI?
accurately the process of DNA cloning?
a. ATTGGC
a. set of laboratory procedures that consist of
b. GAATTC
amplifying a fragment of DNA in vitro
c. CTTAAG
b. set of laboratory procedures that consist of
d. TATGCG
isolation of a DNA fragment from a living
e. TTACGG
organism and insertion of it into a plasmid
Answer: d
Bloom’s Taxonomy: Knowledge/Application c. set of laboratory procedures that uses
living cells to make many identical copies
D 15. Which of the following describes most of a DNA fragment
accurately the expression “recombinant DNA”? d. set of laboratory procedures by which a
a. DNA molecule amplified from a given DNA fragment is transferred from a living
organism organism to a chip
b. DNA molecule that contains genetic e. the manipulation of DNA fragments in a
information from more than one organism laboratory using modern techniques of
c. DNA molecule that is moved from one molecular biology
tube to another in a laboratory Answer: c
d. DNA molecule isolated from a specific Bloom’s Taxonomy: Knowledge/Comprehension/Analysis
organism
e. DNA molecule that has been used in a
laboratory for experimental purposes
Answer: b
Bloom’s Taxonomy: Knowledge/Comprehension/Analysis

104 Chapter 10
D 20. A plasmid E 25. Which of the following organisms are most
a. is a small piece of DNA that is often used in biotechnology?
isolated from bacterial chromosomes for a. viruses
cloning purposes. b. yeasts
b. is a big linear piece of DNA that is often c. bacteria
isolated from bacterial chromosomes for d. plants
cloning purposes. e. mice
c. is a small circular DNA in bacteria that is Answer: c
partially associated with the chromosome. Bloom’s Taxonomy: Knowledge
d. is a small circular DNA in bacteria that is M 26. A restriction enzyme is likely to cut which of
completely associated with the the following molecules?
chromosome. a. a single-stranded DNA molecule
e. is a small circular DNA in bacteria that is b. a double-stranded DNA molecule
completely separated from the c. a single-stranded mRNA molecule
chromosome. d. a double-stranded mRNA molecule
Answer: e e. a double-stranded DNA associated with
Bloom’s Taxonomy: Knowledge/Comprehension
histone proteins
M 21. A plasmid may Answer: b
a. accept foreign DNA. Bloom’s Taxonomy: Knowledge/Comprehension/Analysis
b. be taken up by bacteria. M 27. What is the enzyme that is needed to synthesize
c. carry marker genes. a complementary DNA (cDNA) from an
d. replicate independently of bacterial mRNA molecule?
chromosomes. a. complementary DNA polymerase
e. do all of these. b. DNA polymerase
Answer: e c. RNA polymerase
Bloom’s Taxonomy: Knowledge/Comprehension
d. reverse transcriptase
E 22. When a population is made up of individuals e. DNA transcriptase
who are genetically identical, each individual is Answer: d
said to be a __________ of other individuals. Bloom’s Taxonomy: Knowledge/Comprehension
a. homologous M 28. Which of the following molecules may be the
b. clone substrate of DNA ligase?
c. twin a. a single-stranded DNA molecule
d. pair b. a double-stranded DNA molecule
e. homomere c. a single-stranded mRNA molecule
Answer: b d. a double-stranded mRNA molecule
Bloom’s Taxonomy: Knowledge
e. a double-stranded DNA associated with
E 23. Which of the following components is NOT histone proteins
essential for DNA cloning? Answer: b
a. plasmid Bloom’s Taxonomy: Knowledge/Comprehension
b. DNA ligase E 29. Where are reverse transcriptases obtained
c. DNA helicase from?
d. DNA fragment a. fungi
e. restriction enzyme b. bacteria
Answer: c c. viruses
Bloom’s Taxonomy: Knowledge
d. plants
M 24. Which of the following molecules can be used e. algeas
as a cloning vector? Answer: c
a. plasmids Bloom’s Taxonomy: Knowledge
b. restriction enzymes
c. recombinant DNA
d. DNA fragments
e. bacteria
Answer: a
Bloom’s Taxonomy: Knowledge/Comprehension

Biotechnology 105
M 30. Which molecule is used as a template in the M 35. Which of the following is (are) essential for a
production of a cDNA? DNA probe?
a. a single-stranded DNA molecule a. It must be identical to both strands of the
b. a double-stranded DNA molecule targeted DNA sequence.
c. an rRNA molecule b. It must be complementary to one of the
d. a tRNA molecule strands of the targeted DNA sequence.
e. an mRNA molecule c. It must be as long as the targeted DNA
Answer: e sequence itself in order to make sure of a
Bloom’s Taxonomy: Knowledge/Comprehension perfect match.
M 31. After synthesis of a cDNA molecule, what is d. It must be abundant in solution.
the enzyme responsible for the synthesis of the e. It must be all of these.
second strand of DNA? Answer: b
Bloom’s Taxonomy: Comprehension
a. complementary DNA polymerase
b. DNA polymerase E 36. Which of the following procedures is used to
c. RNA polymerase amplify DNA in vitro?
d. reverse transcriptase a. DNA cloning
e. DNA transcriptase b. reverse transcription
Answer: c c. nucleic acid hybridization
Bloom’s Taxonomy: Knowledge/Comprehension d. DNA restriction
E 32. If the complete genetic information of a person e. polymerase chain reaction (PCR)
is isolated from some of his cells, cut with Answer: e
Bloom’s Taxonomy: Knowledge
restriction enzymes, inserted into a cloning
vector, and introduced within bacteria for E 37. Which of the following molecules could be
cloning purposes, what would the set of clones used as a template during a series of
be called containing all the genes of that polymerase chain reactions?
person? a. RNA molecules
a. a genetic library b. complex lipids
b. a genomic library c. DNA molecules
c. a gene library d. carbohydrates
d. a DNA library e. proteins
e. a nucleotide library Answer: c
Answer: b Bloom’s Taxonomy: Knowledge
Bloom’s Taxonomy: Knowledge
M 38. Which of the following lists contain ingredients
E 33. What do biologists use in order to identify that are essential for PCR?
clones that contain the fragment of interest? a. nucleotides, DNA template, Taq
a. probes polymerase, and primers
b. markers b. nucleotides, DNA template, DNA ligase,
c. templates and primers
d. tags c. restriction enzymes, DNA template, Taq
e. followers polymerase, and primers
Answer: a d. nucleotides, DNA template, Taq
Bloom’s Taxonomy: Knowledge polymerase, and plasmids
E 34. What are the properties of DNA probes? e. nucleotides, DNA template, DNA ligase,
I. They have to be labeled with a tracer. and plasmids
II. They are short chains of nucleotides. Answer: a
Bloom’s Taxonomy: Knowledge/Comprehension
III. They have to match the targeted DNA
sequence. E 39. In DNA molecules, which of the following
a. I and II bonds are disrupted at high temperatures?
b. II and III a. ionic bonds
c. I only b. hydrogen bonds
d. I, II, and III c. polar covalent bonds
e. III only d. nonpolar covalent bonds
Answer: d e. peptide bonds
Bloom’s Taxonomy: Knowledge Answer: b
Bloom’s Taxonomy: Knowledge

106 Chapter 10
M 40. During a polymerase chain reaction, M 43. In step A, which of the following molecules
a. a DNA template is mixed with primers, would be responsible for the production of
nucleotides, and Taq polymerase. molecule #2?
b. the sample is heated in order to allow the a. DNA polymerase
two strands of DNA to separate b. nucleases
completely. c. nucleotide polymerase
c. some of the primers base-pair with d. RNA polymerase
template DNA. e. reverse transcriptase
d. Taq polymerase performs DNA synthesis Answer: e
from the primers. Bloom’s Taxonomy: Knowledge/Application
e. all of these occur. M 44. In step B, which of the following molecules
Answer: e would be responsible for the production of
Bloom’s Taxonomy: Knowledge/Comprehension
molecule #3?
a. DNA polymerase
Examine the following figure and answer questions 41-45.
b. nucleases
c. nucleotide polymerase
d. RNA polymerase
e. reverse transcriptase
Answer: a
Bloom’s Taxonomy: Knowledge/Application
E 45. Taken together, #2 and #3 constitute a
a. double-stranded DNA molecule.
b. nucleotide.
c. double-stranded RNA molecule.
d. polypeptide.
e. polysaccharide.
Answer: a
Bloom’s Taxonomy: Application

STUDYING DNA
E 46. Which of the following is unique to each
E 41. In the figure shown, #1 represents a human?
__________ molecule. a. DNA fingerprint
a. nucleotide b. DNA footprint
b. complementary DNA c. RNA fingerprint
c. messenger RNA d. RNA footprint
d. restriction DNA e. both DNA footprint and RNA footprint
Answer: a
e. amino acid
Bloom’s Taxonomy: Knowledge
Answer: c
Bloom’s Taxonomy: Application E 47. In all humans, about __________ percent of
E 42. In the figure shown, #2 represents a the genome is the same.
__________ molecule. a. 95
a. nucleotide b. 98
b. complementary DNA c. 99
c. messenger RNA d. 99.5
d. restriction DNA e. 100
Answer: c
e. amino acid
Bloom’s Taxonomy: Knowledge
Answer: d
Bloom’s Taxonomy: Application

Biotechnology 107
E 48. About __________ percent of the human E 53. In which of the following cases would DNA
genome is unique to each individual. fingerprinting be used?
a. 0 a. to verify that two individuals who were
b. 0.5 separated during their childhood are
c. 1 related
d. 2 b. to resolve paternity disputes
e. 5 c. to solve crimes
Answer: c d. to do all of these
Bloom’s Taxonomy: Knowledge e. to do none of these
E 49. Which of the following techniques reveals Answer: d
Bloom’s Taxonomy: Knowledge
differences in the number of tandem repeats
among individuals? E 54. Short tandem repeats on __________ may be
a. PCR used to determine genetic relationships among
b. DNA amplification male relatives and descendants.
c. DNA footprinting a. the Y chromosome
d. DNA fingerprinting b. chromosome # 3
e. RNA footprinting c. the X chromosome
Answer: d d. chromosome # 8
Bloom’s Taxonomy: Knowledge e. chromosome # 20
M 50. During a DNA fingerprinting procedure, Answer: a
Bloom’s Taxonomy: Knowledge
tandem repeats of four or five nucleotides are
a. isolated from the cell. E 55. Which of the following elements is NOT
b. inserted into a cloning vector. involved in DNA fingerprinting?
c. copied with a reverse transcriptase. a. primers
d. isolated from the cell and inserted into a b. Taq polymerase
cloning vector. c. electrophoresis
e. amplified by PCR. d. DNA template
Answer: e e. none of these are involved
Bloom’s Taxonomy: Knowledge/Comprehension Answer: e
Bloom’s Taxonomy: Knowledge
E 51. After obtaining DNA fragments by PCR, a
small fraction of the PCR product is loaded and M 56. In which of the following cases would DNA
ran into a(n) fingerprinting NOT be used?
a. transformation machine. a. resolution of paternity disputes
b. test tube. b. expression of a human insulin gene into a
c. electrophoresis gel. bacterium
d. cloning set. c. to determine if two individuals living in
e. restriction gel. two different continents are closely related
Answer: c genetically
Bloom’s Taxonomy: Knowledge d. resolution of a crime
E 52. Which of the following is CORRECT about e. diagnosis of infectious diseases
electrophoresis? Answer: b
Bloom’s Taxonomy: Knowledge/Comprehension/Analysis
a. It separates fragments of DNA according
to their sizes. M 57. DNA sequencing is a method
b. It uses a semisolid gel. a. of determining the order of fatty acids in a
c. In an electrophoresis gel, short DNA DNA.
fragments migrate faster than large DNA b. of determining the order of saccharides in
fragments. a DNA.
d. A current is applied during electrophoresis. c. of determining the order of amino acids in
e. All of these are true. a DNA.
Answer: e d. of determining the order of nucleotides in
Bloom’s Taxonomy: Knowledge a DNA.
e. of determining the order of triglycerides in
a DNA.
Answer: d
Bloom’s Taxonomy: Knowledge/Comprehension

108 Chapter 10
E 58. Sequencing the entire human genome would D 63. Which of the following organisms contains
a. help to identify genes that are essential in more genes that a human?
the defense system of the human body. a. a cow
b. open ways to study human genetic b. a horse
diseases. c. a mouse
c. open ways for gene therapy. d. a dog
d. do all of these. e. a chicken
e. do none of these. Answer: c
Answer: d Bloom’s Taxonomy: Knowledge
Bloom’s Taxonomy: Knowledge
D 64. Which of the following organisms contains
M 59. Which of the following techniques would allow almost as many genes as a human?
health professionals to determine that a given a. a cow
patient has a mutation in the gene encoding b. a horse
alpha-amylase? (Alpha-amylase is the enzyme c. a dog
responsible for breaking down starch d. a chimpanzee
molecules into smaller blocks) e. a roundworm
a. sequencing Answer: e
b. polymerase chain reaction Bloom’s Taxonomy: Knowledge
c. DNA fingerprinting E 65. Which of the following subdisciplines of
d. cloning biology focuses on understanding the genetic
e. all of these composition of organisms?
Answer: a a. physiology
Bloom’s Taxonomy: Comprehension/Application
b. molecular biology
E 60. Which of the following organisms has their c. genomics
genome completely sequenced? d. biochemistry
a. human e. embryology
b. mouse Answer: c
c. dog Bloom’s Taxonomy: Knowledge
d. chicken E 66. Which of the following subdisciplines of
e. all of these biology focuses on comparing genetic
Answer: a information of different species?
Bloom’s Taxonomy: Knowledge
a. molecular biology
E 61. How many genes does the human genome b. biochemistry
contain? c. botany
a. about 5,000 genes d. genomics
b. about 10,000 genes e. embryology
c. about 20,000 genes Answer: d
d. about 30,000 genes Bloom’s Taxonomy: Knowledge
e. about 7,000 genes D 67. DtxR is a protein that controls expression of
Answer: c genes involved in acquisition of iron in the
Bloom’s Taxonomy: Knowledge
bacterium Corynebacterium diphteriae. IdeR, a
E 62. In which year was the human genome homologous of that protein, was found in
published? Mycobacterium tuberculosis playing the same
a. 2003 role. Which of the following approaches was
b. 2001 used in order to achieve that result?
c. 1999 a. DNA fingerprinting
d. 1997 b. polymerase chain reaction
e. 1995 c. comparative genomics
Answer: b d. analytic electrophoresis
Bloom’s Taxonomy: Knowledge e. DNA cloning
Answer: c
Bloom’s Taxonomy: Comprehension/Application/Synthesis

Biotechnology 109
GENETIC ENGINEERING M 73. Which of the following organisms produce
E 68. Which of the following processes consist of genetically engineered chymotrypsin, used in
transferring genetic information from one the food industry?
organism to another? a. a pig
a. genetic information hybridization b. a bacterium
b. polymerase chain reaction c. a yeast
c. comparative genomics d. a sheep
d. DNA fingerprinting e. a cow
e. genetic engineering Answer: b
Answer: e Bloom’s Taxonomy: Knowledge
Bloom’s Taxonomy: Knowledge D 74. Traditionally, chymotrypsin used in the food
D 69. Which of the following procedures are possible industry was obtained from
with genetic engineering? a. mold fermentation.
a. express a human gene in a bacterium b. yeast fermentation.
b. transfer a human protein into a sheep c. sheep stomachs.
c. transfer a human genome into a bacterium d. bacterial fermentation.
d. express a human amino acid in a mouse e. calf stomachs.
e. all of these Answer: e
Answer: a Bloom’s Taxonomy: Knowledge
Bloom’s Taxonomy: Comprehension/Analysis/Synthesis E 75. Which of the following organisms is (are)
E 70. Which of the following organisms is (are) easily modified by genetic engineering?
being used routinely in genetic engineering for a. bacteria and yeasts
production of drugs? b. bacteria and molds
I. rats c. yeasts and mice
II. yeasts d. mold and mice
III. bacteria e. mold, mice, and bacteria
a. I and II Answer: a
b. I and III Bloom’s Taxonomy: Knowledge
c. II and III E 76. Which of the following statements is (are)
d. I, II, and III true?
e. III only a. Modified Agrobacterium tumefaciens is
Answer: c used to deliver recombinant DNA into
Bloom’s Taxonomy: Knowledge some plants.
M 71. In which of the following organisms is human b. Agrobacterium tumefaciens infects many
insulin massively produced? plants including potatoes, peas, and beans.
a. a pig c. Agrobacterium tumefaciens is used to
b. a bacterium improve the nutritional value of some food
c. a chimpanzee plants.
d. a sheep d. Soybean, squash, and potatoes can be
e. a cow modified by using Agrobacterium
Answer: b tumefaciens.
Bloom’s Taxonomy: Knowledge e. All of these are true.
Answer: e
M 72. When was the insulin produced by transgenic
Bloom’s Taxonomy: Knowledge
bacteria approved by the FDA (Food and Drug
Administration)? M 77. Researchers introduce DNA into plants by
a. 1972 using
b. 2000 I. bacteria
c. 1998 II. electric shocks
d. 1982 III. chemical shocks
e. 1995 a. I only
Answer: d b. II only
Bloom’s Taxonomy: Knowledge c. III only
d. II and III
e. I, II, and III
Answer: e
Bloom’s Taxonomy: Knowledge/Comprehension

110 Chapter 10
E 78. Genetic engineering is applied in plants for
which of the following reasons?
a. confer tolerance to dry and hot seasons
b. confer tolerance to low temperature
c. improve nutritional value
d. improve yields
e. all of these
Answer: e
Bloom’s Taxonomy: Knowledge
M 79. In agriculture, the use of GMOs is regulated by
a. the Food and Drug Administration
b. the American Heart Association
c. Greenpeace
d. the U.S. Department of Agriculture
e. the National Institute of Health and the
U.S. Department of Agriculture.
Answer: d
Bloom’s Taxonomy: Knowledge
D 80. The term “biotech barnyards” most likely
refers to
a. the production of human products by farm
animals.
b. the development of new species of farm
animals.
c. the increase of milk, egg, wool, etc.,
production.
d. ultramodern methods of rearing more
animals on less feed.
e. the creation of transgenic animals that can
produce several products.
Answer: a
Bloom’s Taxonomy: Comprehension/Analysis
E 81. Transgenic goats are now able to produce
proteins used to treat
a. bacterial infections and cystic fibrosis.
b. heart attacks and viral infections
c. heart attacks and cystic fibrosis.
d. bacterial infections and heart attacks.
e. bacterial infections, heart attacks, and
cystic fibrosis.
Answer: c
Bloom’s Taxonomy: Knowledge
M 82. The transferring of an organ from one species
to another is called
a. xenotransplantation.
b. eugenics.
c. illegal.
d. genomics.
e. cloning.
Answer: a
Bloom’s Taxonomy: Knowledge
GENETICALLY MODIFIED HUMANS
D 83. Gene therapy consist of
a. replacing the genomes of an individual
with a brand new set of genomes with the
goal of treating a genetic defect.
b. replacing a defective gene in an individual
with a normal gene with the goal of
treating a genetic disorder.
c. transferring a modified gene into an
individual with the goal of treating a
genetic defect.
d. transferring a normal or a modified gene
into an individual with the goal of treating
a genetic defect.
e. replacing a defective enzyme in an
individual with a normal enzyme with the
goal of treating a genetic disorder.
Answer: d
Bloom’s Taxonomy: Comprehension/ Analysis
M 84. In gene therapy, the transfer of recombinant
DNA to a patient may be done via
a. viral vectors.
b. bacterial vectors.
c. microinjection.
d. intramuscular injection.
e. intradermal injection.
Answer: a
Bloom’s Taxonomy: Knowledge/Comprehension
M 85. Which of the following genetic problems is
NOT being addressed by gene therapy?
a. Down syndrome
b. cystic fibrosis
c. hemophilia
d. immune disorder
e. cancers
Answer: a
Bloom’s Taxonomy: Knowledge/Comprehension
D 86. Gene therapy has risks because
a. the virus-injected gene may be
overexpressed in the patient.
b. the virus-injected gene may cause
allergies.
c. it is impossible to predict where a virus-
injected gene will insert into
chromosomes.
d. the virus-injected gene may stay outside of
the cells and never be taken up.
e. of all of these.
Answer: c
Bloom’s Taxonomy: Comprehension/Analysis
Biotechnology 111
Selecting the Exception M 92. Four of the five statements listed below are true
of cloned DNA. Select the EXCEPTION.
E 87. Four of the five answers listed below are a. The plasmid used is the cloned vector.
ingredients of a polymerase chain reaction. b. Identical copies are produced.
Select the EXCEPTION. c. Cloned DNA is produced by reverse
a. DNA template transcriptase.
b. primers d. Multiple copies are produced.
c. DNA ligase e. Cloned DNA is manufactured in bacteria.
Answer: c
d. Taq polymerase
Bloom’s Taxonomy: Knowledge/Comprehension
e. nucleotides
Answer: c
Bloom’s Taxonomy: Knowledge Matching Questions
M 88. Four of the five answers listed below are
involved in engineering and expressing a M 93-99. Match the following letters with the number
recombinant DNA molecule. Select the with which they best correspond.
EXCEPTION.
a. genetically modified organism
a. DNA ligase
b. Golden rice
b. DNA polymerase
c. DNA fingerprinting
c. restriction enzymes
d. genetic engineering
d. plasmid
e. sequencing
e. bacteria
f. eugenics
Answer: b
g. polymerase chain reaction
Bloom’s Taxonomy: Knowledge/Comprehension
D 89. Four of the five answers listed below are 93. _____ an individual’s unique array of short
related to DNA fingerprinting. Select the tandem repeats
EXCEPTION. 94. _____ an organism whose genome has been
a. electrophoresis deliberately modified
b. nucleotides 95. _____ method of determining the order of
c. DNA polymerase nucleotides in a DNA molecule
d. primers 96. _____ method that rapidly generates many copies
e. DNA ligase of a specific DNA fragment
Answer: e 97. _____ type of cereal that has been genetically
Bloom’s Taxonomy: Knowledge/Comprehension/Analysis engineered to provide a fair amount of
M 90. Four of the five answers listed below are crop beta-carotene to the consumer
plants that have been genetically modified. 98. _____ process by which deliberate changes are
Select the EXCEPTION. introduced into an individual’s genome
a. radish 99. _____ idea of deliberately improving the genetic
b. corn qualities of the human kind
c. banana Answers: 93. c, 94. a, 95. e, 96. g, 97. b, 98. d, 99. f
d. squash Bloom’s Taxonomy: Knowledge/Comprehension
e. cassava
Answer: a
Bloom’s Taxonomy: Knowledge
M 91. Four of the five answers listed below are
aspects of the “molecular toolkit.” Select the
EXCEPTION.
a. DNA ligase
b. reverse transcriptase
c. restriction enzyme
d. replicase
e. DNA polymerase
Answer: d
Bloom’s Taxonomy: Knowledge/Comprehension

112 Chapter 10
Classification Questions
The following items are related to DNA recombinant
technology. Answer questions 100-108 in reference to that
topic.
a. restriction enzymes
b. recombinant DNA
c. plasmids
d. clones
e. restriction site
f. reverse transcriptase
g. cDNA
h. a DNA library
i. DNA polymerase
E 100. _____ bacterial population containing
thousands or millions of identical copies of one
to several genes
M 101. _____ collections of DNA fragments produced
by restriction enzymes and incorporated into
cloning vectors
D 102. _____ molecule that needs primers,
nucleotides, and a DNA molecule as a template
in order to fulfill its function
M 103. _____ is from a virus and catalyzes reactions to
construct DNA strands from mRNA.
M 104. _____ molecules which function to chop up
DNA molecules
M 105. _____ molecule that is synthesized from an
mRNA
E 106. _____ represents a genetic information
molecule that contains DNA from more than
one organism
M 107. _____ specific region of DNA that is
recognized by a DNA-chopping protein
M 108. _____ the pieces of DNA that are moved by
genetic engineering from one organism to
another are first incorporated into these
Answers: 100. d, 101. h, 102. i, 103. f, 104. a, 105. g,
106. b, 107. e, 108. c
Bloom’s Taxonomy: Knowledge/Comprehension/Analysis

Biotechnology 113
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