Government Holkar(Model, Autonomous) Science

College, Indere (M.P.)

Department of Biochemistry
B.Sc. program
Session: 2023-24

Program: Diploma A Class: B.Sc. IV Sem

Total Marks
Paper Title Total
External Min CCE Min
Min

Major Intermediary 60 21 40 14 35
Theory Metabolism
Minor
Clinical Biochemistry 60 21 40 14 35
Theory
Minor Biochemical
60 21 40 14 35
Practical investigations
Open Basics of nutrition 60 21 40 14 35
Elective
Vocational-A Medical Diagnostics-II 60 21 40 14 35
Theory
Vocational-A Medical Diagnostics-II 100 35
Practical Lab

Vocational-B Nutrition and Dietetics

60 21 40 14 35
Theory I

Vocational-B Nutrition and Dietetics 100 35

Practical II Lab

6.9.23

72
 Government Holkar (Model, Autonomous)Science
Programme- B.Sc.
(Biochemistry-Major)
1 Course Code
2 Course Title
3
Pre- requisite (if any) To study this course, a student must have had the subject
Course Learning
Outcomes (CLO)
Credit Value
6 Totai Marks
College, Indore (M.P.)
Department of Biochemistry
Part A-Introduction
Class -B.Sc. IV Semester Year- 2023 Session- 2023-24
Course Type - Major
S4-BCHIT
Intermediary Metabolism
Biochemistry in First Year.
On completion of the course the students will be able to:
o Connect the different metabolic pathway for
carbohydrates for yielding energy in cell.
o Illustrate the pathway for transport of electron in ETC.
o Relate the need and process of oxidation of lipid and
biosynthesis of fatty acid in living system.
Categorize amino acid based on its metabolism.
Develop insight about metabolism of nucleotide and
porphyrin.
Theory-6 Credit
Formative Assessment (CCE) 40 Marks Minimum Pass
Summative Assessment (End Semester Marks -35
Exam) -60 Marks
Total 40+60= 100 Marks
Part - B Content of the Course

Total no. of lectures - As per UGC rules (1 Credit = 15 Lectures)

S. No. Topics No. of
Lectures
Introduction, general features of metabolis1m and its importance. Principles 20
of thermodynamics, free energy, standard free energy.
Biological oxidation-reduction reactions, redox potential, ATP and high
energy phosphate compounds.
Carbohydrate metabolism: Reactions and energetics of Glycolysis and
Tricarboxylic acid cycle (TCA), substrate level phosphorylation.
Regulation of Glycolysis and TCA cycle. Alcoholic and lactic acid
1ermentation.
Reaction and significance of Gluconeogenesis, Glycogenesis,
Glycogenolysis, Pentose Phosphate Pathway.

73
S

Class -B.Sc. IV Semester Year- 2023 Session- 2023-24

Programme- B.Sc.
(Biochemistry-Major)
Course Type (Subject) - Major
Course Code S4-BCHIT
Course Title Intermediary Metabolism

Structure of mitochondria, electron transport chain (ETC) and its sequence. 16

Site of ATP production in ETC. inhibitors of ETC.
Hypothesis of mitochondrial oxidative phosphorylation, inhibitors and
uncouplers of oxidative phosphorylation.
Transport of reducing potential in mitochondria.
18
III Introduction, hydrolysis of triacylglycerol.
Transport of fatty acid in mitochondria, B-oxidation of saturated fatty acids,
ATP production from fatty acids oxidation. Biosynthesis of saturated and
unsaturated fatty acids.
Metabolism of ketone bodies, oxidation of unsaturated and odd chain fatty
acids.
Outlines of biosynthesis of triglycerides and important phospholipids,
glycolipids, sphingolipids, and cholesterol.
Regulation of cholesterol metabolism.

IV General reactions of amino acid metabolism: Transamination, oxidative 18

deamination, decarboxylation.
Outline of degradation and biosynthesis of amino acids (Glycine, Serine,
Methionine, Glutamic acid, Aspartic acid, Arginine, Tyrosine, Proline).
Glycogenic and ketogenic amino acids,Urea cycle.
Sources of atoms in purine and pyrimidine molecules. Biosynthesis and 18
degradation of purines and pyrimidines.
Reguiation of purine andpyrimidine biosynthesis.
Porphyrin metabolism: Biosynthesis and degradation of porphyrins.
Production of bile pigments.
Keywords: Redox Reaction, Electron Transport Chain, Triacylglycerol, Transamination, Purine,
Porphyrin

74
 Class -B.Sc. IV Semester Year- 2023 Session- 2023-24
Programme- B.Sc.
(Biochemistry-Major)
Course Type (Subject) Major
Course Code S4-BCH|T
Course Title Intermediary Metabolis1m

Part - C Learning Resources

Text Books, Reference Books, Other Resources
Suggested Readings:
Text Books:
1. Nelson D. L., Michael M. Cox, "Lehninger Principles of Biochemistry", International
Edition, CBS publishers, 2004, 4th Ed.
2. Berg J.M. Tymoczko J.L., Stryer L. "Biochemistry", W.H. Freeman, I1995, 4th Ed.
3. Murray R.K., Granner D.K. Mayes P.A. ,Rodwell V.W., "Harper's Biochemistry",
Prentice Hall International Inc, 2009, 28th Ed.
4. Geoffrey L. Zubay, "Biochemistry", McGraw Hil. 1997.
5. West R., ToddB., Mason M., Bruggen R.V. Textbook of Biochemistry"-Amerind
Publishing Co. Pvt.Ltd., 1986, 4th Ed.
6. Satyanarayana U., Chakrapani U.Biochemistry", Elsevier, 2013, 4th edition.
7. Voet, Donald, Voet, Judith &Pratt, Charlotte, "Biochemistry", Wiley & Sons, Inc. (New
Jersey), 2013, 4th ed.
8. Chatterjea M.N. and Shinde R.. "Textbook of Medical Biochemistry", Jaypee
Publications, 2012, 8th Ed.
9. BookS published by M.P. Hindi Granth Academy, Bhopal
Suggested Digital Platforms Web Links:
1. https:/ndl.itkgp.ac.in
2. https:/lepgp.inflibnet.ac.in
3. http:/len.wikibooks.org/wiki/Biochemistry
4. https:l/www.mphindigranthacademy.org

Suggested Equivalent Online Courses:

1. https:l/nptel.ac.in
2. https://swayam.gov.in
3. https://ugcm00cs.inflibnet.ac.in

75
 Programme- B.Sc. Class -B.Sc. IV Semester Year- 2023 Session- 2023-24
(Biochenmistry-Major)
Course Type (Subject) - Major
Course Code S4-BCHIT
Course Title Intermediary Metabolism

Part - DAssessment and Evaluation

Internal Assessment: Continuous External Evaluation(Summative Assessment):
Comprehensive Evaluation (CCE) End Semester Exam:60 Marks
Formative Assessment: 40 Marks Time: 03 hours
Formative Assessment shall be based on -
Quiz, Seminar, Presentation, Written test,
Case Study, Project, Assignment etc.
The division of marks is as follows:
Test I 20 Marks Section (A): 5 Objective 5x 1=5
Questions (1 mark each)

Test II 20 Marks Best two test Marks Section (B): 5 Short Questions 5 x7=35
= (20+ 20)
outof eight questions (200
words each) (7 Marks each)
Test III 20Marks Section (C): Two long questions 2 x 10 = 20
out of four questions (500
Words each) (10 Marks each)
Total Internal 40 Marks Total External Evaluation 60 Marks
Assessment (CCE) (Theory) Marks (A+B+ C)
Marks

1. For Major, Minor, Open Elective, Foundation and Vocational

Dwill be as per the scheme of marks given. Courses, Part
Note:
The student should secure 35% marks in Internal
2.
External Evaluation (theory) combined. Assessment (CCE) and

76
 Government Holkar (Model, Autonomous) Science
College, Indore (M.P.)
Department of Biochemistry
Part A - Introduction

Programme- B.Sc. Class B.Sc. IV Semester Year- 2023 Session- 2023-24

(Biochemistry-Minor)
1 Course Code
2 Course Title
3 Pre - requisite (if any)
4 Course Learning
Outcomes (CLO)
5 Credit Value
6 Total Marks
Total no. of lectures - As per UGC rules (1 Credit = 15 Lectures)
S. No.
Basic concept of clinical biochemistry, definition and its scope in diagnosis
Course Type - Minor
S4-BCH2T
Clinical Biochemistry
To study this course, a student must have had the subject
Biochemistry in First Year.
On successful completion of this course the student will be able
to:
Gain conceptsof assessing human physiology using
various biological fluids.
o Develop employability skill in collection and preservation
of biological fluid.
o Criticize the functionality of organs based on level of
different plasma enzymes.
o Evaluate the organ functions using specific organ
function tests.
o Seek employability in diagnostic and research institutes
Theory4 Credit
Formative Assessment (CCE) - 40 Marks Minimum Pass
Summative Assessment (End Semester Marks -35
Exam) -60Marks
Total40+60= 100 Marks
Part- B Content of the Course
No. of
Topics
Lectures
6

of diseases.
Standard units and abbreviation of biological constituents.
Quality control: Manual versus automated processes in a clinical laboratory.

Collection and preservation: Blood, Serum, Plasma, Urine and Cerebrospina! 12

fluid (C3F).
Importance of biochemical analysis ofblood, urine and CSF.
Normal values for important constituents in the blood (plasma/serum), CSF
and Urine.

77
Programme- B.Sc. Class B.Sc. IV Semester Year- 2023 Session- 2023-24
(Biochemistry-Minor)
Course Type (Subject) - Minor
Course Code S4-BCH2T
Course Title Clinical Biochemistry
III Definition and importance: functional plasma enzymes, non-functional 15
plasma enzymes and isozymes.
Enzyme pattern in health and diseases with special reference to serum
alkalineand acid phosphatase. GOT, GPT, LDH, CPK, acetyl choline
esterase and lipase.
IV Hypo and hyperglycemia, ketosis, glycosylated hemoglobin (HbA IC). Lipid 15
malabsorption and steatorrhea, role of lipid profile in diagnosis of disease.
Inborn errors of amino acid metabolism- alkaptonuria, phenylketonuria,
albinism, Gout and hyperuricemia.
Evaluation of functional tests of liver, kidney, pancreas, heart and related 12
disorders.
Bile Pigments-Chemical nature and physiological significance, Direct and
indirect bilirubin and pathophysiology of Jaundice.
Keywords: Diagnosis of disease, Body fluids,Isoenzymes, Hyperglycaemia, Organ function test

78
 Programme- B.Sc. Class B.Sc. IV Semester Year- 2023 Session- 2023-24
(Biochemistry-Minor)
Course Type (Subject) - Minor
Course Code S4-BCH2T
Course Title Clinical Biochenmistry

Part -C Learning Resources

Text Books, Reference Books, Other Resources
Suggested Readings:
Text Books:
I. Chatterjea M. N., Shinde R., *Textbook of Medical Biochemistry,Jaypee Publications.
Medical publishers, 2012. 8th Ed.
2. Nelson D. L., Cox Michael M., "Lehninger Principles of Biochem istry", International
Edition, CBS publishers, 2004, 4th Ed.
3. Murray R.K., Granner D.K.,Mayes P.A., Rodwell V.W., "Harper's Biochemistry",
Prentice Hall International Inc, 2009, 28th Ed.
4. Zubay Geoffrey L., *Biochemistry", McGraw Hill, 1997.
5. Gupta S.N.,"Textbook of Biochemistry", Rastogi publications, 2015, 2nd edition.
6. BergJ. M., Tymoczko J. L., Stryer L., "Biochemistry", W.H. Freeman, 1995.4th Ed.
7. Powar C.B. and Chatwal G.R., Biochemistry, Himalaya Publishing House, 2017, 5th
Edition.
8. Satyanarayana U., Chakrapani U.,"Biochemistry", Elsevier, 2013, 4th edition.
9. HallJ.E. and Hall M.E., "Guyton and Hall Textbook of Medical Physiology", Elsevier
India, 2017, 2nd Edition.
10. Vasudevan D. M., SreekumariS., Kannan Vadyanathan, Textbook of Biochemistryfor
Medical Students", Jaypee Publisher, 2016, 8th edition.
11. Books published by M.P. Hindi Granth Academy, Bhopal
Suggested Digital Platforms Web Links:
1. https://ndl.iitkgp.ac.in
2. https:/lepgp.inflibnet.ac.in
3. http:llen. wikibooks.orgwiki/Biochemistry
4. https://www.mphindigranthacademy.org

Suggested Equivalent Online Courses:

I. https:l/nptel.ac.in
2. https://swayam.gov.in
3. https://ugcmoocs.inflibnet.ac. in

79
 Class -B.Sc. IV Semester Year- 2023 Session- 2023-24
Programme- B.Sc.
(Biochemistry-Minor)
Course Tvpe (Subject) Minor
Course Code S4-BCH2T
Course Title Clinical Biochemistry

Part - D Assessment and Evaluation

Internal Assessment: Continuous External Evaluation (Summative Assessment):

Comprehensive Evaluation (CCE) End Semester Exam:60 Marks
Formative Assessment: 40 Marks Time: 03 hours

Formative Assessment shallbe based on -

Quiz, Seminar, Presentation, Written test,
Case Study, Project, Assignment etc.
The division of marks is as follows:
Test I 20 Marks Section (A): 5 Objective 5x l=5
Questions (1 mark each)

Best two test Marks Section (B): 5 Short Questions 5x7=35

Test II 20 Marks
(20 + 20) out of eight questions (200
words each) (7 Marks each)

Test III 20 Marks Section (C): Two long questions 2 x 10 =20

out of four questions ( 500
Words each) (10 Marks each)

Total Internal 40 Marks Total External Evaluation 60 Marks

Assessment (CCE) (Theory) Marks (A+B+ C)
Marks

For Major, Minor, Open Elective, Foundation and Vocational Courses, Part
1. Dwill be as per the scheme of marks given.
Note:
The student should secure 35% marks in Internal Assessment (CCE) and
2 External Evaluation (theory) combined.

13
80
 Government Holkar (Model, Autonomous) Science
College, Indore(M.P.)
Department of Biochemistry
Part A- Introduction Practical

Program: B.Sc. Class: B.Sc. IV Semester Year- 2023 Session- 2023-24

(Biochemistry-Minor)
Course Type(Subject) - Minor
Course Code S4-BCH2P
2 Course Title Biochemical investigation
3 Pre-requisite (if any) To study this course, a student must have had the subject
Biochemistry in First Year.
4. Course Learning The student who completes this course will be able to:
Outcomes (CLO) o Develop technical skills towards various tests performed
for the diagnosis of diseases.
o Handle clinical samples and analysevarious constituents
of these biological samples.
Attain knowledge of organ function tests.
5. Credit Value Practical-2 credits

Part B-Content of the Course

Total no. of lectures -30 hours
Lab Assignments
1. Separation of plasma and serum from blood.
2. Determination of normal and abnormal constituents of urine.

3 Estimation of blood glucose by Nelson Somogyi method.

4 Estimation of protein from serum by biuret and Lowry methods.
5 Estimation of serum albumin and determination of A/G ratio.

6 Determination of cholesterol in serum by Zak's method.

7. Determination of SGOT and SGPT activity.
8. Determination of acid and alkaline phosphatase activity in serum.
9. Estimation of conjugated and unconjugated bilirubin in serum.
10. Estimation of creatinine in serum and urine by Jaffe's method.

Keyword: Plasma, Serum, Urine, Cholesterol, SGPT, SGOT, Alkaline Phosphata7e, Bilirubin,
Creatinine

6-32.3
ey
 Programme- B.Sc. Class B.Sc. I\ Semester Year- 2023 Session- 2023-24
(Biochemistry-Minor)
Course Type (Subject) Minor
Course Code S4-BCH2P
Course Title Biochemical investigation

Part C- Learning Resources

Textbooks, References Books, Other resources
Suggested readings:
Text Books:
1. Plummer D.T."An Introduction to Practical Biochemistry", Tata McGraw Hills Publishing
Company, 2017, 3rd edition.
2. Sadasivan S. Manickam A., "Biochemical Methods", New Age international Publication,
2018, 3rd edition.
3. Swarup N., Pathak S. and Arora S.. "Laboratory techniques in Modern Biology", Kalyani
Publishers, 2004.
4. Gupta R.C. and Bhargava S., "Practical Biochemistry", CBS Publishers & Distributors,
2006, 4th edition.
5. Chakravarthy Anuradha, Kanai L Mukherjee, "Medical Laboratory technology (Vol. III)
Procedure Manual for Routine Diagnostic Tests", McGraw HillIndia, 2017,3rd edition.
6. Varley Harold, "Practical Clinical Biochemistry", CBS Publisher, 2005, 4th edition.
7. Soundravally Rajendiran, "Biochemistry Practical Manual", Elsevier India, 2019, 1st
edition.
8. Sood Ramnik, "Text book of Medical Laboratory technology", Jaypee brothers, 2015,2nd
edition.
9. Bocks published by M.P.Hindi Granth Academy, Bhopal
Suggestive Digital platformsweb Links:
1. https://nd1.iitkgp.ac.in
2. https:/epgp.inflibnet. ac.in
3. htp://len.wikibooks.org/wiki/Biochemistry
4. https://www.mphindigranthacademy.org
Suggested Equivalent Online Course:
1. https://nptel.ac.in
2. https:/swayam.gov.in
3. https:/ugcmoocs.inflibnet.ac.in

82
 Programme- B.Sc. Class - B.Sc. IV Semester Year- 2023 Session- 2023-24
(Biochermistry-Minor)
Course Type (Subjcct) - Minor
Course Code S4-BCH2P
Course Title Biochemical investigation

Part D- Assessment and Evaluation

Suggested Continuous Evaluation methods:

Internal Assessment/Formative Examination(A): 40 Marks

Lab Record 15 Marks
Attendance in the Lab 05 Marks

Assignments (It can be in different modes) 20 Marks

End Semester External Evaluation (B): 60 Marks

Viva Voce on Practical 10 Marks

Practical Record File 10 Marks

Experiments 40 Marks
Total Marks (A+B) (40+60 =100
Marks)

Awdi.

83
 Government Holkar (Model, Autonomous) Science
College, Indore (M.P.)
Department of Biochemistry
Part A - Introduction

Programme- B.Sc. Class B.Sc. IV Semester Year- 2023 Session- 2023-24

(Biochemistry-Open
Elective)
Course Type -Open Elective
Course Code S4-BCH3T
2 Course Title Basics of nutrition
3 Pre - requisite (if any) This course can be opted as open elective by the students of
following subjects: Open for all.
4 Course Learning On completion of the course the students will be able to:
Outcomes (CLO) Gain an insight into the biochemistry of major nutrients
and role of physiologically important biomolecules.
Grasp the underlying changes during diseases, infections
and special pathological conditions and correlate the role
of diet in management of various diseases.
Plan therapeutic diets for various diseases.
Credit Value Theory 4 Credit
Total Marks Formative Assessment (CCE) 40 Marks Minimum Pass
Summative Assessment (End Semester Marks -35
Exam)- 60 Marks
Total 40+60= 100 Marks

Part- B Content of the Course

Total n0. of lectures - As per UGCrules (1 Credit= 15 Lectures)
S. No. Topics No. of
Lectures
Definition of nutrition, food and its relation to health, Basic food groups: 12
energy-giving foods, bodybuilding foods and protective food. Nutritional
importance of Macronutrients -Carbohydrates, Proteins and Fats.

Outline of Nutritional importance. 12

Daily requirement and deficiency disorders of Fat and water-soluble
vitamins.
Daily requirement and deficiency disorders of Minerals (Calcium,
Phosphorus, Sodium,Potassium, lodine, and Iron).
Balanced diet, food guide pyramid, principles and need for menu planning. 12
Recommended dietary allowances (RDA), and Specific gnamic actjon
(SDA).

84
 Class -B.Sc. IV Semester Year- 2023 Session- 2023-24
Programme- B.Sc.
(Biochemistry-Open
Elcctive)
Course Type (Subjeet) Open Elective
Course Code S4-BCH3T
Course Title Basics of nutrition

Basal metabolic rate (BMR). Factors affecting BMR, and Energy

requirementsfor variousage groups.
A brief outline of Protein calorie malnutrition, Starvation and Obesity.
IV Role of Dietician, Routine hospital diets. Modification of diet: principle and 12
purpose.
Brief Introduction of Major National Nutrition Programs. Food Fortification,
supplementary feeding.
Role of diet in the management of diseases (Diabetes, Heart Disease, 12
Hypertension, Obesity, liver, kidney disorders, Diarrhea, Dysentery,
typhoid, malaria).
Keywords: Macronutrients, Micronutrients, Energy, Meal Planning, Dietary Management

85
 Programme- B.Sc. Class B.Sc. IV Semester Year- 2023 Session- 2023-24
(Biochemistry-Open
Elective)
Course Type (Subject) Open Elective
Course Code S4-BCH3T
Course Title Basics ofnutrition

Part -C Learning Resources

Text Books, Reference Books,Other Resources
Suggested Readings:
Text Books:
1. Antia, F.P. and Abraham, Clinical Dietetics and Nutrition. P. Oxford University Press,
Delhi. 4th edition 2002, ISBN- 10 9780195664157.
2. Davidson and Passmore, Human Nutrition and Dietetics, Churchill Livingstone 1986. 8h
revised edition.
3. ShubhanginiA. Joshi, Nutrition and Dietetics, McGraw hilleducation India. 2017. ASIN
9339220153.
4. Bamji, M.S., Rao, N.P. and Reddy, V. Textbook of Human Nutrition, Oxford & IBH
Publishing Co. Pvt. Ltd., New Delhi
5. Chatterjea M.N. and Shinde R., Textbook of Medical Biochemistry, Jaypee Brothers,
Medical publishers. 2012. ISBN 9789350254844.
6. Swaminathan M.S., Advanced Textbook onFood and Nutrition. Vol. I& II. The Bangalore
Printing & Publishing Co. Ltd. Bangalore. 2008.
7. Khanna, K., Gupta, S. and Seth, R., Textbook of Nutrition and Dietetics, Phoenix Pub.
House Pvt. Ltd.,New Delhi. 6th edition. 2016.ISBN 10-8188901539.
8. Robinson, C.H. and Lawler, M.R., Normal and Therapeutic Nutrition. Macmillan, New
York. 1990.
9. Sabarval B., Applied Nutrition And Health, Common, Wealth, 1999.
10. Gopalan.C, Rama Sastri, B.V, and Balasubramian, S.C. Nutritive Value of Indian Foods.
NIN, IC.
11. Books published by M.P. Hindi Granth Academy, Bhopal
Suggested Digital Platforms Web Links:
1. https://ndl.iitkgp.ac.in
2. https:/lepgp.inflibnet.ac.in
3. htp://en.wikibooks.org/wiki/Biochemistry
4. htps://www.mphindigranthacademy.org

Suggested Equivalent Online Courses:

1. https://nptel.ac.in
2. https://swayam.gov.in
3. https:llugcmoocs.inflibnet.ac.in

86
 Class -B.Sc. IV Semester Year- 2023
Programme- B.Sc. Session- 2023-24
(Biochemistry-Open
Elective)
Course Type (Subject) Open Elective
Course Code S4-BCH3T
Course Title Basics of nutrition

Part - D Assessment and Evaluation

Internal Assessment: Continuous External Evaluation (Summative Assessment):

Comprehensive Evaluation (CCE) End Semester Exam:60 Marks
Formative Assessment: 40Marks Time: 03 hours

Formative Assessment shall be based on -

Quiz, Seminar, Presentation, Written test,
Case Study, Project, Assignment etc.
The division of marks is as follows:
Test I 20 Marks Section (A): 5 Objective 5x l=5
Questions (1 mark each)

Test II 20 Marks Best twotest MarksSection (B): 5 Short Questions 5x7=35

=(20 +20) out of eight questions (200
words each) (7 Marks each)

Test III 20 Marks Section (C): Two long questions 2 x 10 =20

out of four questions (500
Words each)(10 Marks each)
Total Internal 40Marks Total External Evaluation 60 Marks
Assessment (CCE) (Theory) Marks (A+B+ C)
Marks

1 For Major, Minor, Open Elective, Foundation and Vocational Courses, Part
Dwill be as per the schemne of marks given.
Note: The student should secure 35% marks in Internal Assessment (CCE) and
2.
External Evaluation (theory) combined.

87
 Government Holkar (Model, Autonomous) Science
College, Indore (M.P.)
Department of Biochemistry
Part A - Introduction
Programme- B.Sc. Class - B.Sc. IV Semester Year- 2023 Session- 2023-24
(Biochemistry-Vocational-A)
CourseType - Vocational-A
1 Course Code S4-BCH4T
Course Title Medical Diagnostics-II
3
Pre-requisite (if any) Open for All
4 Course Learning On successfulcompletion of this course the studentwill be able
Outcomes (CLO) to:
Criticize the functionality of organs based on level of
different plasma enzymes.
Evaluate the organ functions using specific organ
function tests.
Seek employability in diagnostic and research institutes
o Develop technical skills towards various tests performed
for the diagnosis of diseases.
Handle clinical samples and analyze various constituents
of these biological samples.
Explain diseases and diagnostic medical techniques used.
5 Credit Value Theory -3 Credit
6 Total Marks Formative Assessment (CCE) 40 Marks Minimum Pass
Summative Assessment (End Semester Marks -35
Exam) - 60 Marks
Total 40+60= 100 Marks
Part- B Content of the Course
Total no. of lectures - As per UGC rules (1 Credit = 15 Lectures)
S. No. Topics No. of
Lectures
Functional and Nonfunctional plasma enzymes 08
Diagnostically important enzymes.
Detoxification, Phase I and Phase II reactions.
II Liver fncion test, Bilirubin- direct and indirect, Van den Bergh test and its 08
clinical significance, Bile pigments-chemical nature and physjetagical
significance. Jaundice, fatty liver
Kidney function test
1. Test for Urea
2. Test for creatinine and creatinine çlearance
 Class - B.Sc. IV Semester Year- 2023 Session- 2023-24
Programme- B.Sc.
(Biochemistry- Vocational-A)
Course Type (Subject) - Vocational-A
S4-BCH4T
Course Code
Course Title Medical Diagnostics-||

3 Test for proteins

Gastric function test

Carcinogenesis, characteristics of cancerous cells, agents promoting 08

carcinogenesis. Free radicals in a biological system, Antioxidants.

V Techniques in medical diagnostics

Introduction and applications of:
X-Ray
2 Computed Tomography (CT-Scan)
3 Electrocardiography (ECG)
4 Semi-automatic analyser
Spectrophotometer
Keywords:Detoxification, Van den Bergh test, Jaundice, creatinine clearance, ECG

89
 Class - B.Sc. IV Semester Year- 2023 Session- 2023-24
Programme- B.Sc.
(Biochemistry- Vocational-A)
Course Type (Subject) Vocational-A
S4-BCH4T
Course Code
Medical Diagnostics-||
Course Title

Part - C Learning Resources

Text Books, Reference Books, Other Resources

Suggested Readings:
Text Books:
Publications,
1. Chatterjea M. N., Shinde R., "Textbook of Medical Biochemistry, Jaypee
Medical publishers, 2012, 8th Ed.
International
2. Nelson D. L., Cox Michael M., "Lehninger Principles of Biochemistry",
Edition, CBS publishers, 2004, 4th Ed.
Biochemistry", Prentice
3. Murray R.K., Granner D.K., Mayes P.A., Rodwell V.W., "Harper's
Hall International Inc, 2009, 28th Ed.
4. Zubay Geoffrey L., Biochemistry", McGraw Hill, 1997. edition.
5. Gupta S.N., "Textbook of Biochemistry", Rastogi publications, 2015, 2nd Ed.
6. Berg J. M., Tymoczko J. L., Stryer L, Biochemistry", W.H. Freeman, 1995.4th Sth Edition.
Powar C.B. andChatwal G.R., Biochemistry, Himalaya Publishing House, 2017,
7.
8. Satyanarayana U., ChakrapaniU., Biochemistry", Elsevier, 2013, 4th edition. Elsevier India,
9. Hall J.E. and HallM.E., "Guyton and Hall Textbook of Medical Physiology",
2017, 2nd Edition. Biochemistry for
10. Vasudevan D. M., Sreekumari S., Kannan Vadyanathan, "Textbook of
Medical Students", Jaypee Publisher, 2016, 8th edition.
11. BookS publishedby M.P. Hindi Granth Academy, Bhopal
Suggested Digital Platforms Web Links:
1. https://nd1.iitkgp.ac.in
2. https:llepgp.inflibnet.ac.in
3. http:/len.wikibooks.org/wiki/Biochemistry
4. https://www.mphindigranthacademy.org

Suggested Equivalent Online Courses:

1. https://nptel.ac.in
2. https://swayam.gov.in
3. https:/lugcmoocs.inflibnet.ac.in

90
 Class -B.Sc. IV Semester Year- 2023 Session- 2023-24
Programme- B.Sc.
(Biochemistry- Vocational-A)
Course Type (Subject) - Vocational-A
Course Code S4-BCH4T
Course Title Medical Diagnostics-||

Part- D Assessment and Evaluation

Internal Assessment: Continuous External Evaluation (Summative Assessment):

ComprehensiveEvaluation (CCE)/ End Semester Exam:60 Marks
Formative Assessment: 40 MarkS Time: 03 hours

Formative Assessment shall be based on -

Quiz, Seminar, Presentation, Written test,
Case Study, Project, Assignment etc.
The division of marks is as follows:
Test I 20 Marks Section (A): 5 Objective 5x |=5
Questions (1 mark each)

Test II 20 Marks Best two test MarksSection (B): 5 Short Questions 5x7=35
= (20 + 20) out of eight questions (200
words each) (7 Marks each)

Test III 20 Marks Section (C): Two long questions 2 x 10 = 20

out of four questions (500
Words each) (10 Marks each)

40 Marks Total External Evaluation 60 Marks

Total Internal
Assessment (CCE) (Theory) Marks (A+B+ C)
Marks

1
For Major, Minor, Open Elective, Foundation and Vocational Courses, Part
D will be as per the scheme of marks given.
Note: The student should secure 35% marks in Internal Assessment (CCE) and
2. External Evaluation (theory) combined.

91
 Government Holkar (Model, Autonomous) Science
College, Indore(M.P.)
Department of Biochemistry
Part A- Introduction Practical

Class: B.Sc. IV Semester Year- 2023 Session- 2023-24

Programn: B.Sc.
(Biochemistry- Vocational-A)
Course Type - Vocational-A
1. Course Code S4-BCH4P
2. Course Title Medical Diagnostics-II Lab
3. Pre-requisite (if any) Open for All
4. Course Learning On successful completion of this course, the student will be able
Outcomes (CLO) to:
ldentify the components of serum, including serum
albu.ain and globulins, and their significance in health
assessnment.
Summarize the biuret method and how it quantifies
protein levels in serum.
Analyse the clinical implications of protein levels,
albumin levels, and the A/G ratio in patient health.
Evaluate and critique the diagnostic methods and
equipment observed during the field trip, considering
their efficiency and accuracy.
5 Credit Value Practical-lcredit

Part B-Content of the Course

Total no. of lectures30 hours
Lab Assignments
Separation of plasma and serum from blood.
2 Estimationof protein from serum by biuret method.
3. Estimation of serumalbumin and determination of A/G ratio.
4 Determination of SGOT andSGPT activity.
5. Determination of acid and alkaline phosphatase activity in serum.
6. Estimation of conjugated and unconjugated bilirubin in serum.
7. Estimation of creatinine in serum and urine by Jaffe's method.
8. Field trip: Vicitto a Medical Diagnostic Centre to study about different diagnostic
techniques and present a report.
Keyword: Plasma, Serum, Biuret, SGOT and SGPT, Phosphatase, Jaffe's method
 Class - B.Sc. IV Semester Year- 2023 Session- 2023-24
Programme- B.Sc.
(Biochemistry-Vocational-A)
Course Type (Subjcct) Vocational-A
Course Code S4-BCH4P
Course Title Medical Diagnostics-I| Lab

Part C- Learning Resources

Textbooks, References Books, Other resources
Suggested readings:
Text Books:
1. Chatterjea M. N., Shinde R., "Textbook of Medical Biochemistry, Jaypee Publications,
Medical publishers, 2012, 8th Ed.
2. Nelson D. L., Cox Michael M., "Lehninger Principles of Biochemistry", International
Edition, CBS publishers, 2004, 4th Ed.
3. Murray R.K., Granner D.K., Mayes P.A., RodwellV.W., "Harper's Biochemistry", Prentice
HallInternational Inc, 2009, 28th Ed.
4. Zubay Geoffrey L., "Biochemistry", McGraw Hill, 1997.
5. Gupta S.N, "Textbook of Biochemistry", Rastogipublications, 2015, 2nà edition.
6. Berg J. M., Tymoczko J. L., Stryer L., Biochemistry", W.H. Freeman, 1995.4th Ed.
7. Powar C.B.and Chatwal G.R., Biochemistry, Himalaya Publishing House, 2017, Sn Edition.
8. Satyanarayana U., Chakrapani U., "Biochemistry", Elsevier, 2013, 4th edition.
9. HallJ.E. and HallM.E, "Guyton and HallTextbook of Medica! Physiology'",EIsevier India,
2017, 2nd Edition.
10. Vasudevan D. M., SreekumariS., Kannan Vadyanathan, "Textbook of Biochemistry for
Medical Students, Jaypee Publisher, 2016, &ih edition.
11. Books published by M.P.Hindi Granth Academy, Bhopal
Suggestive Digital platforms web Links:
1. https:/l/nd.itkgp.ac.in
2. https:/lepgp.inflibnet. ac.in
3. http:/len.wikibooks.org/wiki/Biochemistry
4. https://www.mphindigranthacademy.org
Suggested Equivalent Online Course:
1. https:/nptel.ac.in
2. https://swayam.gov.in
3. https:llugcmoocs.inflibnet.ac.in

93
 Class B.Sc. IV Semester Year- 2023 Session- 2023-24
Programme- B.S.
(Biochemistry-Vocational-A)
Course Type(Subject) \Vocational-A
Course Code S4-BCH4P
Course Title Medical Diagnostics-llLab

Part D- Assessment and Evaluation

Suggested Continuous Evaluation nethods:

Internal Assessment/Formative Examination(A): 40 Marks

Lab Record 1S Marks

Attendance in the Lab 05 Marks

Assignments (It can be in different modes) 20 Marks

End Semester External Evaluation (B): 60 Marks

Viva Voce on Practical 10Marks
Practical Record File 10Marks
Experiments 40 Marks
Total Marks (A+B) (40 + 60 =100
Marks)

wali...

94
 Government Holkar (Model, Autonomous)Science
College, Indore (M.P.)
Department of Biochemistry
Part A - Introduction

Programme- B.Sc. Class - B.Sc. IV Semester Year- 2023 Session- 2023-24

(Biochemistry-Vocational-B)
Course Type - Vocational-B
1 Course Code S4-BCHST

2 Course Title Nutrition and Dietetics-II

3 Pre-requisite (if any) Open for All

4 Course Learning
Outcomes (CLO)
5 Credit Value
6 Total Marks
On successful completion of this course the student will be able
to:
Provide an understanding of the composition of various
foodstuffs.
o Familiarize students with changes occurring in various
foodstuffs as a result of processing and cooking.
Enable students to use theoretical knowledge in various
food processing methods.
o Provide insight about common food adulterants.
Theory-3 Credit
Formative Assessment (CCE) 40 Marks Minimum Pass
Summative Assessment (End Semester Marks -35
Exam) 60 Marks
Total 40-+60= 100 MarkS

Part- B Content of the Course

Total no. of lectures - As per UGC rules (1 Credit =15 Lectures)

S. No. Topics No. of
Lectures
I Food and Energy 10
Basic concept of energyexpenditure, units of energy, measurementof energy
expenditure by direct and indirect calorimetry, basal netabolic rate (BMR)
and its measurement. Composition of basic foods: cereals, pulses,
vegetables, fruits, egg, meat, fish, poultry, milk and milk products.
Composition of balanced diet.

Food processing 07
Effect of cooking & heat processing cn the nutritive value of foods.
Processed supplementary foods. Loss of nutrients during pesing ynd
cooking, naturally occurring anti-nutrients.

95
 Class - B.Sc. IV Semester Year- 2023 Session- 2023-24
Programme- B.Sc.
(Biochemistry- Vocational-B)
Course Type Vocational-B
Course Code S4-BCH5T
Course Title Nutritionand Dietetics-|I
14
Food Spoilage
and cereal
Food spoilage: causes, types, effects on the following: Cereal
products, sugar and sugar products, vegetables and fruits, meat and meat
products, fish, egg and poultry, milk and milk products,canned foods.
Microbial intoxication and infections: Sources of contamination of food,
toxin production and physiological action, sources of infection of food by
pathogenic organisms, symptoms, and method of contro l.
Relevance of microbiologystandards for food safety. Food borne diseases an
their prevention.
08
IV Food preservation
Food preservation: definition, objectives, and principles of food
preservation. Different methods of food preservation.
Preserved Products: Jam, Jelly, Marmalade, Sauces, Pickies. Squashes,
Syrups-types, composition and manufacture, selection, cost, storage, uses
and nutritional aspects.

Food adulteration and food additives 06

Food adulterant: definition, types and their effect, common food adulterant.
Food additives: definition, role, and types. Health risk of food additives.

Keywords: Food groups, Malnutrition, RDA, Dietetics. BMI

96
 Class - B.Sc. IV Semester Year- 2023 Session- 2023-24
Programme- B.Sc.
(Biochemistry- Vocational-B)
CourseType - Vocational-B
Course Code S4-BCHST
Course Title Nutrition and Dietetics-|I

Part -CLearning Resources

Text Books, Reference Books, Other Resources
Suggested Readings:
Text Books:
1. Dietetics: B Srilakshami. New Age International (P) Lid. Publishers 3rd Edition,2000
2. Nutrition and Dietetics: Shubhangini AJoshi, Tata McGraw Hill Publishing Co. Ltd.
New Delhi, 1992.
3. Dietetics: Srilakshmi, B. 1997. New Age lnternational (P) Ltd, Publishers, New Delhi.
4. Principles of Nutrition and Dietetics: Swaminathan, M. 1997. The Bangalore Printing and
Publishing Co. Ltd, Bangalore. (1997reprinted)
5. Textbook of Nutrition and Dietetics: Khanna, K., Gupta, S., Pass, S.J, Pass, S.J, Seth, R.,
Mahan, R. and Puri, S. 1997. Phoenix Publishing House Pvt. Ltd, New Delhi.
6. M. Swaminathan, Handbook of Food and Nutrition.
7. Nutritive value of Indian Foods: Gopalan C, Rama Sastri &Balasubramanian S.C.
National Institution Nutrition, 1993.
8. Books published by M.P. HindiGranth Academy, Bhopal
Suggested Digital Platforms Web Links:
1. https:l/ndl.itkgp.ac,in
2. https://epgp.inflibnet.ac. in
3. htp:/en.wikibooks.org/wiki/Biochemistry
4. https:llwww.mphindigranthacademy.org

Suggested Equivalent Online Courses:

1. https:/nptel.ac.in
2. https:/lswayam.gov.in
3. https://ugcmoocs.inflibnet.ac.in

97
 Programme- B.Sc. Class B.Sc. IV Semester Year- 2023 Session- 2023-24
(Biochenmistry- Vocational-B)
Course Tvpe - Vocational-B
Course Code S4-BCHST
Course Title Nutrition and Dietetics-||

Part- DAssessment and Evaluation

Internal Assessment: Continuous External Evaluation (Summative Assessment):
Comprehensive Evaluation (CCE) End Semester Exam:60 Marks
Formative Assessment: 40MarkS Time: 03 hours
Formative Assessment shall be based on
Quiz, Seminar, Presentation, Written test,
Case Study, Project, Assignment etc.
The division of marks is as follows:
Test I 20 Marks Section (A): 5 Objective 5x 1=5
Questions (1 mark each)

Test II 20 Marks Best two test Marks Section (B):5 Short Questions 5x7=35
(20 + 20) out of eight questions (200
words each) (7 Marks each)
Test III 20 Marks Section (C): Two long questions 2x 10= 20
out of four questions (500
Words each)(10 Marks each)
Total Internal 40 Marks Total External Evaluation 60Marks
Assessment (CCE) (Theory) Marks (A+B+C)
Marks

1. For Major, Minor,Open Elective, Foundation and Vocational Courses, Part

Note:
D will be as per the scheme of marks given.
2.
The student should secure 35% marks in Internal Assessment (CCE) and
External Evaluation (theory)combined.

98
 Government Holkar (Model, Autonomous)Science
College, Indore (M.P.)
Department of Biochemistry
Part A- Introduction Practical
Program: B.Sc. Class: B.Sc. IV Semester Year- 2023 Session- 2023-24
(Biochemistry- Vocational-B)
Course Type - Vocational-B
1. Course Code S4-BCHSP
2. Course Title Nutrition and Dietetics-lILab
3. Pre-requisite (if any) Open for All
4 Course Learning On successfulcompletion of this course the student will be able
Outcomes (CLO) to:
Recall and list the common methods and tests used for
the identification of carbohydrates and proteins in food
samples.
Execute cooking processes such as pressure cooking,
microwave cooking, steaming, grilling, and deep-fat
frying, ensuring food safety and quality.
o Evaluate the accuracy of adulterant detection tests and
make informed judgments regarding food quality and
safety.
Collaborate with peers to develop supplementary food
products
5 Credit Value Practical-lcredit

Part B-Content of the Course

Total no. of lectures - 30 hours
Lab Assignments
ldentification of carbohydrate in given sample.
2. ldentification of protein in given sample.
3. Detection of food adulterant in different food items

4 Process involved in cooking: pressure cooking, microwave, steaming, grilling, deep

fat frying.
5. Visit to food industry/confectionary/lab.
6. Preparation of supplementary food for different age group and their nutritional
significance
Keyword: food adulterant, Grilling, Deep Fat Frying, confectionary, supplementary food
Programme- B.Sc. Class - B.Sc. IV Semester Year- 2023 Session- 2023-24
(Biochemistry-Vocational-B)
Course Type Vocational-B
Course Code S4-BCHSP
Course Title Nutrition and Dietetics-||Lab

Part C- Learning Resources

Textbooks, References Books, Other resources
Suggested readings:
Text Books:
1. Dietetics:BSrilakshami, New Age International (P) LId. Publishers 3rdEdition,2000
2. Nutrition and Dietetics: Shubhangini AJoshi, Tata McGraw Hill Publishing Co. Ltd., New
Delhi, 1992.
3. Dietetics: Srilakshmi, B. 1997.New Age International (P) Ltd, Publishers, New Delhi.
4. Principles of Nutrition and Dietetics: Swaminathan, M. 1997. The Bangalore Printing and
Publishing Co. Ltd, Bangalore. (1997 reprinted)
5. Textbook of Nutritionand Dietetics: Khanna, K., Gupta, S., Pass, S.J, Pass, S.J, Seth, R.
Mahan, R. and Puri, S. 1997. Phounix Publishing House Pvt. Ltd, New Delhi.
6. M. Swaminathan, Handbook of Food and Nutrition.
7. Nutritive value of Indian Foods: Gopalan C, Rama Sastri& Balasubramanian S.C. National
Institution Nutrition, 1993.
8. Books published by M.P. HindiGranth Academy, Bhopal
Suggestive Digital platforms web Links:
1. https:/lndl.iitkgp.ac.in
2. https:lepgp.inflibnet.ac.in
3. http://en.wikibooks.org/wiki/Biochemistry
4. https://www.mphindigranthacademy.org

Suggested Equivalent Online Course:

1. https:/nptel.ac.in
2. https://swayam.gov.in
3. https://ugcmoocs.inflibnet.ac.in

100
 Programme- B.Sc. Class B.Sc. IV Semester Year- 2023 Session- 2023-24
(Biochemistry-Vocational-B)
Course Type - Vocational-B
Course Code S4-BCHSP
Course Title Nutrition and Dietetics-|| Lab

Part D- Assessment and Evaluation

Suggested Continuous Evaluation methods:

Internal Assessment/Formative Examination(A): 40Marks

Lab Record 15 Marks

Attendance inthe Lab 05 Marks

Assignments (It can be in different modes) 20 Marks

End Semester External Evaluation (B): 60 Marks

Viva Voce on Practical 10Marks
Practical Record File 10Marks
Experiments 40 Marks
Total Marks (A+B) (40 + 60 =100
Marks)

ati

101