absorbance conc(Mm)

2.883 1000
1.212 500
1.06 250
0.81 100
0.531 50

standard curve
3.5

y = 0.0023x + 0.4213 3
R² = 0.9458 2.5

Absorbance
2
1.5
1
0.5
0
1200 1000 800 600 400 200 0
)concentration (mM