AC university

1st INTERNATIONAL
CONFERENCE
on
RECENT ADVANCES IN CHEMICAL,
PHARMACEUTICAL AND BIOLOGICAL SCIENCES
(RACPABS - 2020)
Or
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zedby
Depar
tmentofChemi
str
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ACHARYANAGARJUNAUNI
VERSI
TY
Nagar
junaNagar-522510,Gunt
ur,A.
P.,I
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a
1st International Conference
on
Recent Advances in Chemical,
Pharmaceutical and Biological Sciences
(RACPABS - 2020)
5th to 7th March, 2020
SOUVENIR
Organized by
Dr. D. Ramachandran
Director - RACPABS 2020
Department of Chemistry
Acharya Nagarjuna University
Nagarjuna Nagar, Guntur – 522 510
Andhra Pradesh, India
Organizing Committee
Profile of the Department of Chemistry
The Department of Chemistry, University College of Sciences, Acharya Nagarjuna
University was the erstwhile department of Chemistry, Andhra University Post graduate
Centre established in 1967. Ever since, it was started eminent professors steered the
Department to great heights. The department has published about 830 research articles in
various national and international journals of repute. As many as 165 research students have
received Ph.D. degrees and about 225 research students receive M.Phil. Degrees. Besides, the
faulty members have presented several papers in various seminars and conferences and
summer inspire programme conducted by Prof.C.Rambabu. Notable among them the “39th
annual convention of Chemists” Dec. 23d to 26th 2002, recently Prof.B.Kesava Rao,
Dr.D.Ramachadran and Dr.B.Hari Babu visited Dubai, Abudabi, Mauritius, Hong Kong ,
Macau and Malaysia, Egypt to present the papers in International Seminars.
The faculty members Prof.N.S.Prakasa Rao, Prof.P.V.V.Satyanarayana,

Prof.B.Syamsundar and Prof.C. Rambabu received Best Teacher awards from Govt. of A.P.
Prof.B.Syamsundar has got prestigious honor by Fellow of Royal Society of Chemistry
(FRSC-London). He was also appointed as Vice-Chancellor of Yogi Vemana University,
Kadapa by Govt. of A.P. in 2013. Dr.D.Ramchandran has got the Outstanding Faculty
Award-2015 and International Best Researcher in Chemistry, RULA Awards, World
Research Council, 15th August 2019 by Venus International Foundation, Chennai.
Dr.R.Ramesh Raju has been elevated as Associate Fellow, Andhra Pradesh Academy of
Sciences and Dr,B.Haribabu received Best Teacher awards from Govt. of A.P during 2019.
At present 7 major research projects are in succession and 10 BSR fellowships, 6

CSIR fellowships and 2 RGNF fellowships are sanctioned by UGC.
The Department of Chemistry got (Rs.128 lakh rupees) the funding under DST FIST
Level-1 program to procure the NMR 400MHz and Densitometer.
The department of chemistry is offering four specializations: Analytical, Inorganic ,

Organic and Forensic Chemistry (SF) in M.Sc.
CONTENTS
S.No Title of the Paper and Author Name P.No.

KEYNOTE ADDRESS
Molecules with Celebrity Status 1
Dr. S. Chandra Sekhar
INVITED TALKS
1. Phytonanotechnology – Anti-diabetic drugs and Possible Mechanisms 2
Prof. P. Visweswara Rao
2. Friccohesity and Survismeter: Theory and Applications in Areas of Chemical, 3
Pharmaceutical, and Biological Sciences
Prof Man Singh
3. Borneon Natural Products: Anti-Inflammation/Anti-Cancer Activities and their Mechanism 4
of Action
Prof. Charles S Vairappan
4. Wetland Degradation And Its Impacts On Natural Resources In Ethiopia- A Review 5
Dr. Lakew W Abachiri
5. Exploring the Prevalence and Risk Factors of Prediabetes in Adult Population of West Shoa 6
Zone, Oromia region, Ethiopia – A Community Based Study
Dr. R. Vinodhini
6. Recent Approaches in Natural Products for New Drug Development 7
Dr. K. Suresh Babu
7. Exploring Synthetic Molecules and the Nano-Bio Platform as Therapeutics Against Drug- 8
Resistant Pathogenic Bacteria
Prof. A. Ramesh
8. Synthesis, spectroscopic and electrical characteristics of alkali sulphophosphate glasses 9
mixed with some d and f block ions
Prof. N. Veeraiah
9. Synthesis of an antipsychotic drug substance Clozapine with a very economic key starting 10
material 2-chloro-benzoic acid
Dr. S. Venkata Rao
10. Design and Synthesis of Novel Bio-active Organophosphorus Compounds 11
Prof. C. Suresh Reddy
11. Multi-Functional Lipid-Dye- Fe@Cnps Conjugates For Biomedical Applications 12
Prof. M. V. Basaveswara Rao
12. Mutagenesis aids in studying pathogenesis and vaccine development against 13
Ehrlichia and Anaplasma species pathogens impacting human and animal health
Prof. Roman R Ganta
13. Nanoscience in Interventional oncology: Opportunities & Challenges 14
Dr. G. Venkateswara Rao
14. Green chemistry Examples and perspectives for future 14
Prof. S. Jena
15. Recent Advances in Hydrogen Fuel Technology 17
Dr. M. Krishnamurthy
16. Strategies For Protecting Chemical, Pharmaceutical & Biological Inventions 17
Dr. T. Pavan Kumar
17. Catalysis Using Functionalized Nanomaterials for the Synthesis of High Value Chemicals 18
Dr. K. V. S. Ranganath
18. Strategic planning of proteins in ionic liquids: Future solvents for enhanced stability of 18
protein against multiple stresses
Prof. P. Venkateshu
19. Electromembrane Extraction- A Novel Extraction Technique for Pharmaceutical, Chemical, 19
Clinical and Environmental Analysis
Dr. M. Vamsi krishna
CHEMICAL ORAL PRESENTATIONS
Volumetric and Accoustic study of 1-Butyl-3-methylimidazolium bis 20
OP-1 (trifluoromethylsulfonyl) imide with 4-methyl-2-pentanone
Dr. Raghavendra Muvva
A Meglumine Catalyst Based Synthesis, Molecular Docking and Anti-oxidant Studies of 21
OP-2 Dihydropyrano[3, 2-b] Chromenedione Derivatives
N. Bakthavatchala Reddy1*, Grigory V Zyryanov1,2, A. Balakrishna3, C. Suresh Reddy4
An effective green and ecofriendly catalyst for synthesis of bis(indolyl)methanes as 22
OP-3 promising antimicrobial agents
Gundala Sravya1*, Grigory V. Zyryanov1,2, V. Padmavathi3, A. Padmaja3
Green One-pot Synthesis of Aminomethylene Bisphosphonates as Antimicrobial and 22
OP-4 Antioxidant Agents
Tellamekala Sreekanth1, Cirandur Suresh Reddy1*
Design of biostable scaffold based on collagen crosslinked by dialdehyde chitosan with 23
OP-5 presence of gallic acid
Pemo Bam, Anindita Bhatta and Ganesan Krishnamoorthy*
Investigations on corrosion studies of Al6061 reinforced with TiO2 metal matrix composites 24
OP-6 in Hydrochloric acid medium
Latha.V1*, H.R.Radha1, P.V.Krupakara2 and R.Lakshmi1
molecular interactions in binary mixtures containing halo-substituted carbonyl compound+ 25
OP-7 xylenes at various temperatures
M.RadhaSirija1, Sk.Jareena2, D. Ramachandran*
green and efficient heterogeneous nanocatalyst for the synthesis of α -aminophosphonates 25
OP-8
S .H. Yasmin1 ,S.Murali1, G.Mohan1 & *C. Suresh Reddy1
Smart Drug Delivery Mechanism Using Nanorobots: The Emerging Tools In Medicinal 26
OP-9
Sikhinam Nagamani1, Dr.G.Muthaiah2, Dr.D.Ramachandran3
Effect Of Temperature On Physicochemical Properties Of Propyl Acetate With 1-Alkyl-3- 27
OP-10 Methyl- Imadazolium Hexaflurophospate
P Bharath a,*, P Suneethab, T.S.Krishnac, D.Ramachandrana
Adsorption Of Disperse Dyes Onto Activated Carbon Prepared From Agricultural Waste 28
OP-11
T.V.Nagalakshmi1*, K.A.Emmanuel2, P. Paul Divakar3
Evaluation of ultrasonic velocity theories on binary liquid mixtures of Propiophenone with 29
OP-12 isomeric xylenes at temperatures T=(303.15 to 318.15)K
Nanduri Gayatri Devi 1 , N.V.N.B.Srinivasa Rao2 ,D.Ramachandran3
Silver decorated reduced graphene oxide nano composite with a valuable biomedicinal 30
OP-13 plant, Kigelia Aficana (KA) for synthesis and its characterization
K. Chandra Mohan, K. Bala Murali Krishna and Dr. B. Hari Babu*
Method Development And Validation Of Venetoclax In Human Plasma By Lc-Esi-Ms/Ms 31
OP-14
P. Haritha *
Evaluation of ultrasonic velocity theories on binary liquid mixtures of Morpholine with 32
OP-15 Amides at temperatures T = (303.15 to 318.15) K
D.Bala, V. Anand Kumar, Dr.D.Ramachandran
Pt-Sn nanoparticles decorated binary poly(amine terminated cyclophosphazene-co- 33
benzidine disulfonic acid)-carbon nanotube composite for methanol electrooxidation in
OP-16
alkaline medium
D.Prasannaa*, S. Ratna Kumaria, Ch. Rajinia, K.Krishnadevi, V.Anuradhaa,c*
Anti-Hyperglycemic Effect Of Chrysin On Glucose Loaded Rats 34
OP-17
S.Kalyani1 , Dr.D.Ramachandran2
Understanding of molecular interactions between Ethyl Acetate and 1-Butyl-3-methyl- 35
OP-18 imidazolium bis(trifluoromethylsulfonyl)imide: A Thermophysical study
P.V.S.S Rama Raoa, T.S.Krishnab,*, D. Ramachandranc,¥
New Rp-Hplc Method Development Validation And Subsequent Forced Degradation 35
Studies For The Simultaneous Estimation Of Chlorpropamide And Phenformin In
OP-19
Pharmaceutical Formulations
B.Sowjanya* & Dr. K. Rambabu┼
Design, Synthesis and Biological Evaluation Of Amide Functionality bearing 1, 2, 4- 36
Thaidazole- 1, 2, 4- Triazole derivatives as Anti- cancer Agents.
OP-20
Yazala Jyothsna Pragathi,1 Reddymasu Sreenivasulu,2 Deekala Veronica,1 Rudraraju
Ramesh Raju*1
Molecular interactions in binary mixtures containing dialkyl carbonate esters with chloro 37
OP-21 toulenes at various temperatures
A. Venkateswara rao1, m. Radha sirija2, N. Venkateswara Rao, d. Ramachandran+
Structural and Spectral Investigations of VO2+ Ion Doped Cd3(BO3)2 Nanocomposites For 37
OP-22 Optoelectronic Applications
P.N.V.V.L.Pramila Rani 1, D.Ramachandran1, R.V.S.S.N.Ravikumar2
Design, Synthesis of Sulphonamide fused 2-(Isoxazol-3-yl)-1H-Imidazoles and their impact 38
OP-23 on Anticancer Activities
B. Ravinaika, P.T.S.R.K.PrasadaRaob, D.Ramachandran*c and M.V. BasaveswaraRaod
Qualitative And Quantitative Analysis Of Water Samples Of Kidney Effected Area Using 39
OP-24 Icpms Technique
T.P.Raju1*, N.Giridhar Babu1, Ch.Ch.Srinivas1,R.R.Vedantam,2 N.Lakshmana Das3
New techniques for the Identification of pollutants of certain elements: A study 40
OP-25
M.V.K.Mehar1, P.Suresh2
Rapid Quantification Of The Apixaban In Dosage Forms By Stability Indicating Reverse 41
OP-26 Phase-High Performance Liquid Chromatography
T.Suresh* & Dr. K. Rambabu┼
Kolleru Lake: Its Present Water Quality Status 41
OP-27
Dr.K.Soujanya¹, Dr.D.Ramachandran²
A new stability- indicating rp-hplc method for the Determination of retigabine in oral 42
OP-28 dosage form
K.Swapna , k.Ravindhranath ,Dr.D.Ramachandran
Study Of Molecular Docking For Isolated Bioflavonoid Compound(S) 43
OP-29
Dr.Chandrashekar.C and Dr.K Soujanya
Design, Synthesis, Hypoglycemic Activity and Molecular Docking Studies of 3-substituted- 44
OP-30 5-[(furan-2-yl)-methylene]-thiazolidine-2,4-dione Derivatives
K. Srikanth Kumar*, A. Lakshmana Rao,
Synthesis and Characterization Of Barium Hydroxide Nanoparticles 45
OP-31
VeronicaDeekala, , Jyothsna Pragathi Yazala, Anitha Kowthalam, RameshrajuRudraraju
An experimental investigation of molecular interactions between [Emim][NTf2] ionic liquid 45
OP-32 & 2-ethoxyethanol a comparison by PFP theory and Computational study
A. Sreenivasa Rao1,2 P.Sowjanya2 & B. Hari Babu2
Biological evaluation of a Benzazepine derivative 9-methoxy-5-oxo-2,3,4,5-tetrahydro-1H- 46
OP-33 benzo[b]azepine -2-carboxylic acid derivatives
Nagaraju Marepu, G.Mahesh, V.Santhoshi Sumana, M.Kanthi Kirani, and Y.Sunandamma
Simple and Efficient Synthesis of Schiff base Derivatives of 2-AminoBenzothiazole and 2- 47
OP-34 Picolylamine
Usharani.P 1 and N Madhavi*
Novel Functional Thiopheno Azomethines as Fluorescent (Turn on) and colorimetric sensor 48
OP-35 for the Cu2+ ion
Koteswara Rao Bandiab, Hongli Baoa*, Lic
Detection of Fenoprofen calcium forced degradation impurities by UPLC‐QTOF mass 49
OP-36 spectrometry
P. Yella Reddy1, Ch. N. S .S Pavan Kumar1and S.Venkat Rao2
Green Chemistry- The Only Way To Ecological Security 50
OP-37
Dr.Ch. Udaya lakshmi1, Dr. V.N.S.R. Venkateswararao2, Smt. M.N.Parameswari3
Synthesis and Characterization of Silver Nanoparticles Using Gomphrena Serrata Plant 51
OP-38 Extract
S. Pavani, Sk. Parveen, R. Sowmya, M. Rama Devi and K. Prasada Rao*
Bioanalytical Method Development and Validation of Venetoclax with internal standard in 52
Plasma by High Performance Liquid Chromatography with Mass Spectrometry:
OP-39
Application to Bioequivalence Study
V.N.V Kishore1, Dr. K. Bala Murali Krishna1 Dr. G.V. Ramana2*
Design, Synthesis And Molecular Docking Studies Of Eif3a Inhibitors As Potential 53
OP-40 Anticancer Agents
Dr.M.Rajasekhar1,G.Mohini1,Dr.R.S.K.Sharma1and Dr.D.Ravi Kumar*
Kinetics and Mechanistic Study of Oxidation of L-Citrulline by periodate in Acidic and 53
OP-41 Basic Media
D. Raga Sudha1, P. Vani2 , G. Nageswara Rao*2
Green-Chemistry (Air Pollution) 54
OP-42
G.Alluraiah
Synthesis of new (±)-1-(4-(3-fluorobenzyloxy)pyrrolidin-3-yl)-4-phenyl-1H-1,2,3-triazole 55
derivatives via Click reaction and study of anti-cancer activity against HCT 116, MDA-
OP-43
MB231, Mia-PaCa2 Cell lines
Gollapudi Ravi Kumar1,2, Hari Babu Bollikolla1*
Removal Of Health Hazardous Pollutants From Wastewaters Using Green Synthesized 56
OP-44 Nanoparticles
Dr. M. Suneetha, Prof. B. Syama Sundar, Prof. K. Ravindhranath
Binary mixtures of Allyl alcohol with various functional groups 57
OP-45 (Thermodynamic and Acoustic properties)
Dheiver F. Santos
Thermodynamic and transport properties of binary liquid mixtures of 2-ethyl-1-hexanol with 58
OP-46 ketones at various temperatures
A. Venkatesulua*, M. Gowrisankarb
Molecular interactions between of Allyl alcohol and various functional groups 58
OP-47 (Benzyl chloride, 3-methylaniline, 3-methoxyaniline and 2,6-dimethylcyclohexanone)
B. Srikantha, D. Ramachandrana,*,
Experimental and theoretical values of sound speeds and viscosities for the binary systems 59
OP-48 of 2,6-dimethylcyclohexanone with hydrocarbons
P. Venkateswarlu.
Thermo physical and Spectroscopic properties of binary liquid systems: acetophenone/ 60
OP-49 cyclopentanone/ cyclohexanone with 3-methoxylaniline
R. Balajia, M. Chandra Shekarb
Influence Of Ligand On The Activity Of Metal Ions In Heterogeneous Water-Oxidation 60

OP-50 Catalysts
Satya Vijaya Kumar Nune
Efficient Synthesis of 8-fluoro-10-methyl-3,4-dihydrobenzo[b][1,6] naphthyridine-2(1H)- 61

OP-51 carboxamide and their cytotoxic activity
Rama Koteshwar Raoa and Shravankumar Kankala*a
Studies on Corrosion Characterization of Aluminium 2024 / Aluminium oxide Metal Matrix 63
OP-52 Composites in Hydrochloric Acid Medium
R.Lakshmi1*, H.R.Radha1, P.V.Krupakara2 and Latha.V1
Development of Magnetic based metal oxide and Graphine Oxide nanocomposites for 64
OP-53 biological, catalytic and dielectric applications
D. Chandra Sekhar1,2, Bhagavathula S Diwakar1, N. Madhavi3*
Fried rice syndrome- A comprehensive conventional and alternative approach to treatment 65
OP-54 using Adhathoda vasica
Dr. Swarna latha kurapati1, chandra mohan kurmarayuni2, dr. Haribabu bollikolla1
Analytical method validation for the determination of 2,6-diamino-5-nitropyrimidin-4(3H)- 65
one impurity in 2-[(2-amino-1,6-dihydro-6-oxo-9H-purin-9-yl)methoxy]-3-hydroxypropyl
OP-55 ester, monohydrochloride drug by UHPLC-MS/MS
Mahammad Ali Shaik1* Prof. Dr. N Devanna2
Synthesis and Spectral Determination of active Antimicrobials of Piperidine Analogues of 66
diphenyl-butanamides
OP-56
Sriramudu. B, B. Satyanarayana, S. Venkat Rao, P. Bharath, K. Govinda Rao,
D.Ramachandran*
Synthesis and Anticancer Evaluation of Benzimidazole Bound Glycosyl Triazole Hybrids 67
OP-57
Vijay Kumar Allam, Brahmeshwari Gavaji*
The Study of intermolecular interactions in {1-butyl-3-methylimidazolium 68
hexafluorophosphate + 1-octyl-2-pyrrolidone} mixture from volumetric, optical, acoustic
OP-58 and spectroscopic measurements.
V. SrinivasaRaoa,c, M. SrinivasaReddyb, B. HariBabuc*
Removal of fluoride from drinking water By lanthanum (iii) treated fly ash 68
OP-59
PTSRK Prasada Rao
RP-HPLC method for the determination of Ranozaline in pharmaceutical formulations. 69
OP-60
Masma Shaik, G.Mahesh, V.Santhoshi Sumana, M.Kanthi Kirani, and Y.Sunandamma
An Experimental Study on Microbial Power module for Waste water treatment and 70
OP-61 Electricity Generation
Dr. D. RamaChandran, Mr. T. Benjamin, Dr. Ch. Rajyalakshmi
Synthesis of new Derivatized Dithizone Ligands and its use in the Estimation of Mercury 71
OP-62 from Waste Water Sample Using UV-Vis Spectrophotometry
A.Tejaswini* and P.V.Krupakra
Copper Mediated Selective Halogenation Of Arylcyanamides: Synthesis of ortho Halo 71
OP-63 Arylcyanamides
S N Murthy Boddapati,a,b Chandra Mohan Kurmarayunia, Hari Babu Bollikolla*a
Cobalt(II) complexes of pyridyl tetrazole ligands with pendant arms amide 75
OP-64 and hydrazide - synthesis, characterization, DNA binding studies
Shaik Mustafa* and Syed Khasim sharif
Synthesis and Characterisation of some new hydrazone derivatives of 3- (2-hydroxyl-5- 72
OP-65 methyl phenyl)-3- phenyl propane)
Ramya Krishna Pallapati1 and Dr. B. Hari babu*1
3-[(3-(trimethoxysilyl)propyl)thio]propane-1-oxy-sulfonic acid: An Efficient Recyclable 73
Heterogeneous Catalyst for the Synthesis of 1H-pyrazolo [1,2-b]phthalazine-5,10-dione
OP-66 derivatives and their biological evaluation
Srinivasa Rao Jetti,*1 Shubha Jain2
New Polymeric copper 2-PicolonicAcid Crystal Structures and its Biology 74
OP-67
Dr. K.mohana rao1 dr. B.kishore babu2 s.v venkat rao1
Synthesis and characterization of some new Imidazolebenzohydrazide derivatives. 75
OP-68
Mannem Gangadhara Rao, Navudu Ramesh and B. Hari Babu*
Antimicrobial activities of some aryl cyanamides and aryl tetrazoleamine derivatives 75
OP-69
Nathani Srinivasa Raoa and Kondraganti Lakshmib
Stability Indicating Assay Method Development And Validation Of Netarsudil And 76
OP-70 Latanoprost By Rp-Hplc And Its Degradation
M.Manoranjani
Effect of pH, Copper Sulphate and Vitamin E on seed germination of Black gram and 77
OP-71 Bengal gram
V. Ravi1, B.Sai kiran2, B.Ajay kumar3, N Madhavi4 *
An analytical method development and validation for the determination of 4,6 dichloro 5- 78
OP-72 nitro-2-(propylthio)pyrimidine in Ticagrelor drug substance by LC-MS
Krishna Moorthy Manchuri1 Gopi Reddy Venkata Subba Reddy2
Synthesis And Anti-Diabetic Activity Evaluation Of Some New Thiazolidinedione 78
OP-73 Derivatives
Ch. Ramya, P. Lakshmi Sowjanya, V. Yugandhar and Dr. Ch. Subramanyam*
Analytical Method Development and Validation for the determination of Lumacaftor using 79
OP-74 RP-HPLC
Syed. Ibrahim Baje1,M.L.N.Raj Gopal2, G.Praveen Chand3, N Madhavi4 *.
Isolation and Characterization Of Active Natural Products From Cordia Dichotoma 80
OP-75
Gone. Swapna and Prof B. Kesava Rao *
Convenient preparation and docking studies of 7-hydroxyflavanone based acrylate 80
OP-76 compounds as potential anti-microbial agents
P. Asha Bhanu, Prof. B.Kesava Rao*,
Synthesis and In-vivo anti-inflammatory activity of cinnamyl esters of phytosterols 81
OP-77
G. Usha Rani & B. Kesava Rao*
Schiff’s Base – Role In Biological Sciences 81
OP-78
Godha Lakshmi1; Hema Naga Chakra2; Prasanthi3, Basaveswara Rao4*
Nuclear Chemistry – Role In Human Lives 82
OP-79
Saidathu, Sarveswara Rao, Basaveswara Rao*
Magnetic Nanoparticles – Role In Theraupetics 83
OP-80
Reyaz; Jayasri; Basaveswara Rao*
Synthesis of Novel Daclatasvir acid analogues for NS5A hepatitis-c virus inhibitor 84
OP-81
Dr. Sd.Khasim Sharif*, Dr. Shaik. Mustafa
A Facile and Green Synthesis of Ag-Graphene Nanocomposite by Hydrothermal Method 85
and its Catalytic Reduction of 4-Nitrophenol
OP-82
Basavaiah Chandu,1 Syed Akhil,1 Syam Sundar Majety,1 Ganesh Majji,2 Vasu babu Ala,3
Hari Babu Bollikolla.4
Simultaneous Method Development and Validation of Trastuzumab and Hyaluronidase- 86
OP-83 Oysk and Its Pharmacokinetic studies with LC-MS/MS
Subba Rao Yarlagadda1, Pavani Y2, Subba Rao Mannam*
Removal of hexavalent chromium ions in synthetic wastewater by novel biocarbon – A way 88
forward for industrial wastewater treatment.
OP-84
Singanan Malairajan1* Sundaram Rethinasabapathy2, Lingaraj Chellappa 1, Sure Veera
Venkatrao3 and Gunnam Nagarjuna4
Approaches to Digitize the Chemical Industries in India 89
OP-85
Anupriya Koneru1 M.Sreelatha2 K.Lavanya3
Development and Validation of the Head Space Gas Chromatography for Simultaneous 90
Quantification of Organic Volatile Impurities in Enrofloxacin API and its Pharmaceutical
OP-86 Dosages
J. Krishna Kishore, K. PrasadaRao*
Novel benzyl imidazolylcarbamates synthesis from α-amido sulphones 91
OP-87
T. Vijaya Durga, K. Santha Kumari, B. Hari Babu*
Study of non-covalent interactions due to steric and electronic effects in the liquid mixtures 92
of N-Methyl-2-Pyrrolidone and alkanamines through thermodynamic and transport studies
OP-88
D. Bala Karuna Kumar* a , N. Shareef a, Y. Subbareddy a, P. Sugandha Kumarb, and G.
Srinivasa Raoc
New Avenues for the Green Economy and Sustainable Future of Science and Technology 93
OP-89
Sk. N. Shareef a D. Bala Karuna Kumar a
Astrochemistry: Overview And Challenges 94
OP-90
D.Prasanna , K.NagaRamyaKrishna and K. Padmalatha
Corrosion Characterization Of Aluminium 7075 - Titanium Nitride Composites 94
OP-91
P.V.Krupakara1* and M.Singanan2
Competitive Adsorption Studies Of Boron And Thallium (I) Ions On To Lignocellulosic 95
OP-92 Carbon
Dr.Ch.S.R.G.Kalyani
New Cytotoxic Icetexane diterpenes from the stem bark of Premna tomentosa 96
OP-93
A. Hymavathi,a,b K. Rekha,a K. Suresh Babua and J. madhusdana Rao.a
Design, Synthesis and Biological Evaluation of Substituted Styryl Incorporated Quinazoline 97
OP-94 Derivatives as Anticancer Agents
Pruthu Kala, Dittakavi Ramachandran*
Luminescence features of erbium ions in ZnO-Ta2O5/Nb2O5/ZrO2-B2O3 glass system 98
OP-95
A. Siva Sesha Reddy* and N. Veeraiah
Influence of iron ions on physical and spectroscopic properties of 98
OP-96 zinc aluminoborate glass system
A. Venkata Sekhar, Ch. Chandrakala, G. Naga Raju*
Studies on spectroscopic properties of Sm3+ ions in sodium antimonite glasses co-doped 99
OP-97 with manganese ions
B. Madhavi, P. Pavani Koteswari Devi and V. Ravi Kumar*
Recyclization Of Green House Gases Into Fuel And Hydrogen Gas Using Catalyst To 100
OP-98 Reduce Global Warming
Sapavat Madhavi
Development of Flavone-Cyanoacetamide Hybrids as Multitarget Directed Ligand against 101
OP-99 Alzheimer’s Disease
A.G. Damu1*, K. Yelamanda Rao1, S. Jeelan Basha1, S. Rajagopal2
Metal free Multi-Component Approach: Preparation of Benzoyl Derivatives 102
OP-100
Bajivali Shaik1, Mohan Seelam,2 Prasad Rao Kammela2*
Degradation of Pesticides by Ferrous doped TiO2 Nanoparticles 103
OP-101
Y. Prashanthi1, T. Nageshwar Rao2, V. Sridhar1
Ru(III) Catalysed Oxidation Of Benzilic Acid By Periodate In Alkaline Medium 103
OP-102
Dr.Neeraja .Valluru, ,Sailaja .Valluru, Dr.V. Phani Kumar
A One-pot synthesis of thiazolo[3,2-a]pyrimidine derivatives, their cytotoxic evaluation and 104
OP-103 molecular docking studies
P. Thriveni, T. Sekhar, and A.Venkateswarlu
Action against chemical traces in food 105
OP-104
Dr.Neeraja .Valluru, ,Sailaja .Valluru, Dr.V. Phani Kumar, Dr.Sudhamasa .B
Studies On Reactions Of 2-Chloroquinoline-3-Carboxaldehy- De With Different 106
OP-105 Nucleophiles
K. Shiva Kumara,b*, A. Naidua and P. K. Dubeya
Thermoacoustic studies in binary liquid mixture of 2-methoxyaniline with ketones at 107
OP-106 various temperatures.”
E.Kalavathia,b#, A. Venkatesulu b,*, M. Gowrisankar
PHARMACEUTICAL ORAL PRESENTATIONS
Paradigm Shift in Treatment using Biologics and Small molecules 108
OP-1
Dr.Anil S. Khile, Ph.D
Anti cancer studies of Aloe sinana root and leaf extracts of Ethiopia with reference to 109
Lung Carcinoma
OP-2
Akalu Terfa1, Anuradha. G1, J. SreeKanth Kumar2, Hailemicahel Tesso1 and
Young-Sik Jung3
Standardization of Trivrut (Operculina turpethum Linn) a miraculous medicinal plant W.S.R 110
OP-3 to phytochemical analysis and pharmacological properties.
Dr.M. Prasanna lakshmi, Dr.P.Sugunajyothy
Potentiation of apoptosis by novel Ir ( ) complexes on triple negative breast cancer cells 111
OP-4
Rajeeva Lochana Panchangam1, Kaushik Chanda*, Venkatraman Manickam
Synthesis, characterisation and biological evalution of multi component reaction products of 112
OP-5 7-chloro-6-fluoro 1,3-benzothiazole 2-isocyanide .
Dr.d.ravi shankar reddy, burra mahesh reddy
Effects of Aegle marmelos (L.) Methanolic Leaf Extracts on biochemical parameters in 112
OP-6 diabetic rats.
Ravi Babu Birudu1, P.Padmavathi*1, M.Sathish Kumar2
Electro-Organic Synthesis of Late Phase Pharmaceuticals 113
OP-7
Dr. Anuradha.G1, Dr. Fedlu Kedir Sabir1, Prof. Tesfaye Biftu1
Evaluation of antidiabetic potential of fenugreek seed in prediabetic patients. 114
OP-8
Genet Alem Geberemeskel1, Tewabech Zewde2 and Kidanu Estifanos3
Evaluation, characterization of forced degradation products of Levetiracetam by LC-MS/MS, 115
OP-9 1H, 13 C & 2D NMR
Gollu Siva sankara Rao1, S. Venkat Rao2
Qualitative analysis of chloroform and ethanol extracts of Citrus medica L. - Pericarp 115
OP-10
Mahesh Kancherla1*, Mohammad Younis Dar1, Zaheer Ahmed N1
Biological Perspective of 2,5-Diamino Thiadiazole Analogues for Antimicrobial Activity – 116
OP-11 Conformation with DFT Studies
Ch. Ravi Shankar Kumar* M.Prasanti1 and Anjali Jha2
Bilayer Tablet Technology: A Novel Approach 117
OP-12
S. Venkateswara Rao, R. Vyshnavi*, Padmalatha Kantamneni
Bioanalytical LC-MS/MS method for Determination and comparison of Selexipag Assay in 118
various Biological materials and its Application to Pharmacokinetics Studies in Rat plasma
OP-13
Namburi L A Amara babu1, Kalyani Koganti1, Babji Palakeeti2, Koduri S V Srinivas3, and
Koya Prabhakara Rao1*
BIOLOGICAL ORAL PRESENTATIONS
Transcriptomic Analysis of Copper Stimulated Indian White Shrimp (Penaeus indicus) 119
OP-1 1
Polaki Suman, 2 T. Samuel David Raj, 2 P. R. Vani
Antimicrobialpotency of silver nanoparticles 120
OP-2 Of euphorbia miliides moul. Stem extract
A.Ch. Pradyutha1, V. Umamaheswara Rao2
Studies on acute toxicity of glyphosate (sl 41%) impact on freshwater fish cirrhinus mrigala 121
OP-3
A.rajeswari, k. Sunita and k. Veeraiah
Synthesis of nanoparticles using Plant extracts: Characterization and biological application 121
OP-4
B. Pavani Bai, M. Sandhya Rani, G. Gowthami, Sudhakar Podha*
natural products and their role in modern medicine 122
OP-5
ch. anithasmruthi, lalitha vinnakota and dr.v.venkata rathnamma
histopathological studies on phosalone induced toxicity in reshwater fish ctenopharyngodon 123
OP-6 idella (grass carp)
Dr. K.Nirmala1 and Dr. V. Venkata Rathnamma2
Phytochemical synthesis of silver nanoparticle synthesis using H. tiliaceus aqueous leaf 124
OP-7 extract and preliminary examination of antioxidant and anticancer properties.
K. Vinay Viswanath1 Dr. P. Sudhakar
Determination of Bt Gene and Bt protein in Bt cotton seeds by using ELISA and PCR 124
OP-8 Screening and Gel electrophoresis.
Dr.K.Suneetha, K.Praveen Kumar,SK.Neha Fatima and SK.Razeena.
Evaluation of thermal and mechanical characteristics of pullulan edible films incorporated 125
OP-9 with polysaccharides and plasticizers
G. V. S. Ramakrishna1,*, P. Sudhakar2
Role Of Bio Informatics In Animals And Drugs 126
OP-10
G.Mounika,A.Anitha and Dr.V.Venkata Rathnamma
Antibacterial activity of Pseudomonas aeruginosavur-z isolated 127
OP-11 from Polluted water of River Musi and Lake Hussain Sagar
Konatham Samuel John and Umamaheswara Rao V*
Studies on efficacy of Encapsulated Potential Probiotics beads against the Vibriosis in 128
OP-12 Shrimps through Oral administration
K. Rajyalakshmi, Syed Shabana, M.Kishore babu, Dr. Alapati Krishna Satya*
Environmental Pollution And Protection 129
OP-13
K. Saicharitha, Sudharani Chalapala and S. Anwar
Antiplasmodial activity of Cleome viscosa against chloroquine sensitive Plasmodium 129
OP-14 falciparum (3D7 strain) and chloroquine sensitive Plasmodium berghei (ANKA)
K. Sunita, G. Sravani, D. Santha Kumari and Amna Ali Alhadad
Over Exploitation Of Groundwater At Coastal Areas And Their Impact On Chemical 131
OP-15 Changes Of Natural Environment
Lalitha Vinnakota, A. Anitha and Dr. V. Venkata Rathnamma
Efficiency of Streptomyces sp. as biocontrol agent for management of fungal diseases in 132
black gram.
OP-16
M.Kishore babu, K. Rajyalaxmi, Dr.D.S.D suman joshi, Luay Kadhim Hanoon, Qasim Turki,
Dr. A. Krishna Satya
Heterologous production of poly-extremo tolerant laccase from halophilic bacteria with its 133
OP-17 application in dye decolorization
Manne Anupama Ammulu, Vinay Viswanath K, Ajay Kumar G, Sudhakar Poda
Chemicals and drugs from Orchids 134
OP-18
Md. Rahamtulla1, J. Ramudu2 and S.M.Khasim1
Effect of physical parameters and nutrient sources on dibenzothiophene biodesulfurization 134
OP-19
activity and growth of the streptomyces sp. Vur ppr 101.
2-Deoxyglycosyl 3-benzoylpropionates as noveldonors for the direct and stereoselective 136
OP-20 synthesisof 2-deoxy-glycosides
Ramakrishna Bandi, Sudharani Chalapala and Srinivasan Chandrasekaran
A Study On Effect Of Metal Ions In Polygalacturonase Production From Pencellium Species 136
OP-21
Ravi Ketipally and M. Raghu Ram
Knowledge And Practice Of Emergence Contraceptives Among Female Students Of Ejere 137
OP-22 Woreda, Oromia Region, Central Ethiopia
Tolera Gudissa Dame
Transcriptional Profiling of Orange Ripening mutant in Tomato(Solanum lycopersicum) 138
OP-23
P. John Elia Prashanth, N. Rajesh, C. Jareena Begum and P. Osman Basha
Outer Membrane Vesicles of Bovine respiratory disease associated Pasteurella multocida 139
OP-24 and Mannheimia haemolytica as Platform in Veterinary Vaccine Technology
*Mirtneh Akalu Yilma
Isolation and screening of antibiotic producing actinomycetes from the rift valley areas of 140
OP-25 Ethiopia
Firew Elias, Sudhamani Muddada and Driba Muelta
Fisheries in transition: Implications for food and nutrition security in India 141
OP-26
Darwin, Ch., P. Padmavathi and Gatreddi Srinu
Title: “Advanced molecular method in disease monitoring for DNA viruses” 141
OP-27
D.N.V.Raja Rajeswara Rao, Jaganmai.G, K.Sabasteen, Rajeswari Jinka
Preliminary phytochemical screening of ethanomedicinal plants Homalium zeylanicum and 142
OP-28 Schrebera swietenioides.
Santosh kumari .M & Ammani.K
Environmental Education And Scientific Approaches In Schools: The Indian Scenario 143
OP-29
Dr. Sudhakar G 1, Swarna latha G2, Dr. P.Brahmaji Rao
Dye Yielding Plants - A treasure of Ethiopia 143
OP-30
Dr. Anuradha.G1, Dr. Teshome Abdo1, Dr. Alemu Gonfa Robi1, J. Sreekanth Kumar2
Nickel stress tolerance and biosorption potential of rhizobia associated with Horse gram 144
[Macrotyloma uniflorum (Lam.) Verdc.]
OP-31
Prabhavati Edulamudi1*, Anthony Johnson Antony Masilamani3, Venkata Ramana Sai
Gopal Divi2, Umamaheswara Rao Vanga1, Veera Mallaiah Konada
Development & Verification Of Lcms/Ms Method For Determination Of N-Nitroso-N- 145
OP-32 Methyl-4-Aminobutyric Acid (Nmba) Content In Valsartan
Dr.Ch.Prasanna1* & Yarram Rama Koti Reddy
Fungal Pathogen Associated With Murraya Koenigii L. 146
OP-33
1Soumya K.R, & Anjali M
IRISIN- Effect On Metabolism 146
OP-34
Sai Bhavani, Sandeep kumar, Sravanthi Malempati
Terpenoids And There Classification 147
OP-35
G.Mounika, Sudharani Chalapala and S. Anwar
Better Protection- Nanofibers In Masks And Respirators 148
OP-36
Murthy, Sarath Kumar, Naga Raju, Sunil, Kishore
Phyto-Chemical Studies And Evaluation Of Antioxidant Properties Of Selected Mangrove 149
Extracts
OP-37
Deepthimahanthi Divya1, G. Beulah2, K. Govinda Rao2, N.S. Sampath Kumar3 and
G. Simhachala
Remediation Of Environmental Pollution—Role Of Nanoparticles 150
OP-38
Mounika, Krishna Tara, Kalavathi, Ammulu, Vardhan
Effect Of Ethion (50%Ec) On Enzymatic Activities Of Sdh And Ldh In Freshwater Fish 151
OP-39 Labeo Rohita (Hamilton)
Dr.Ch. Prasanna,
DNA Barcoding Technology: Implications for Seafood Traceability and Authenticity 152
OP-40
Gatreddi Srinu, P. Padmavathi and Ch. Darwin
Syzygium aqueum for better health and welfare of mankind 152
OP-41
Kethipalli Swapna*, Kethipalli Ravi1 and Babitha Bobba
Hepatoprotective Effect Of Indigenous Foeniculum Vulgare In Ethanol Intoxicated Male And 153
OP-42 Female Albino Rats
Vinodhini Rajamanickam
Synthesis of Silver Nanoparticles from Pseuderanthemum 154
OP-43 malabaricum and Evaluation of Anticancer Activity.
Luay Kadhim Hanoon, M.Kishore babu, Qasim Turki, K. Rajyalaxmi, Dr. A. Krishna Satya
Methylene blue degradation by green synthesized Silver Nanoparticles from Oldenlandia 154
corymbosa
OP-44
K.Raghavendra1,V.UmaMaheswaraRao2,R.Chinthayyanaidu3,K.V.A.Naidu
K.Suryanarayana3,M.Rajeswari3,J.HariHindhuja3 ,J.Dinesh3,T.Pavani3, B.Akhila
Green synthesis of Silver, Copper and Zinc nanoparticles by using Podranea ricasoliana 155
OP-45 (Tanf.) Sprague leaf extract
K. Raghavendra1 and V. Umamaheswara Rao
Recent Advances in Nano-biotechnology for Improvement in Therapeutic Approaches for the 157
OP-46 Treatment of Diabetes
Dr. T. Sujatha
Approach Towards Solid Wastage Management 157
OP-47
B. Satish Babu1, N.Chimpiraiah2, Dr. P. Brahmaji Rao
Green Synthesis of Silver Nanoparticles using Orthosiphon stamineus (Benth) leaf extract 158
OP-48
N. Vijaya Sree*, P.Udaya Sri* & N. RamaRao
Structural investigation of the exopolysaccharide produced by 159
OP-49 Heavy metal resistant Rhizobium radiobacter strain
Pallaval Veera Bramhachari
Mangrove –mediated synthesis and characterization of silver nanoparticles using Acanthus 160
OP-50 ilicifolius plant leaf extract.
Sunila Rani Pudota, Gorrepati Rosaiah *, Nattala Tirupati Swamy
Characterization and Bioactivities evaluation of herbal nanoparticles mediated by the Leaves 161
OP-51 of Tabebuia argentea Bur. & K. Sch.
Nattala Tirupati Swamy, Gorrepati Rosaiah*, Kakumanu Babu and P. Sunila Rani
CHEMICAL POSTER PRESENTATIONS
Reduced Graphene Oxide (rGO) supported Pd-Ru bimetallic nanocomposite for 162
PP-1
High performance Supercapacitor Applications
Y.Veera Manohara Reddya, M. Venua, N. Guru Prakash b, B. Sravani c, O.M. Hussain b, M.
Nagalakshmi Devammad, K.Anitha e, P.Saraswathi, G.Madhavi*a
Studies on the interactions of 1,2,4-Triazines through inverse demand aza-Diels-Alder 163
PP-2 reactions versus aryne mediated domino process as prominent fluorescent materials.
Aluru Rammohan1,2* and Grigory V. Zyryanov1
Green synthesis of diethyl((2-iodo-4-(trifluoromethyl) phenyl) amino) (aryl) methyl) 164
PP-3 phosphonates as potent α-glucosidase inhibitors
Mahammad Sadik Shaik1, Maheshwara Reddy Nadiveedhi1, Suresh Reddy Cirandur1*
Study on partial molar isentropic compressibility for binary mixtures of Propiophenone with 165
PP-4 1-alkanols at various temperatures 303.15K, 308.15K, 313.15K and 318.15K
Nanduri Gayatri Devi 1 , D.Bala2 ,D.Ramachandran3
Synthesis and Biological Evaluation of Amide Derivatives of 166
PP-5 Imidazopyridine as Anticancer Agents
V. Ramesh a*, G. Purna Chander Raoa, D. Ramachandrana, and A. Kalyan Chakravarthyb
Design, Synthesis and Anticancer Evaluation of 167
PP-6 Novel Furan Sulphonamide Derivatives
G. Purna Chander Raoa*, V. Ramesha, D. Ramachandrana, and A. Kalyan Chakravarthyb
Green synthesis, characterization and application study of Zinc Nano particles synthesized 168
PP-7 using aqueous root extract of Sphagneticola trilobata Lin
Sk.Abdul Mathin1*, M.David Raju2, D.Rama Sekhara Reddy3
Determination Of Genotoxic Impurity 2,3-Xylidine In Paracetamol And Mefenamic Acid 169
Gfos Samples Using Rp-Lc
PP-8
Dr. D. Ravi Kumar1, Dr. V. Siva Ramakrishna3, Dr. R.S.K.Sharma1, T.Benjamin2 and Dr. D.
Ramachandran3
Development and Validation of a Method for Simultaneous Estimation of Daunorubicin and 170
PP-9 Cytarabine in rat plasma by LC-MS-MS and its Application in Bioequivalence study
Anita Kethipalli and Ramachandran. D*
Viscosity studies on binary liquid mixtures of Propiophenone with 2-Alkoxyethanols at T = 170
PP-10 (303.15 to 318.15) K
Nanduri Gayatri Devi 1 , P.Bharath2 ,D.Ramachandran3
Volumetric behaviour of binary liquid mixtures Containing alcohols 171
PP-11
Sk.Haseena Begum1 and K.Bhagya Lakshmi2
Simultaneous determination of Amlodipine and Irbesartan in formulations using RP-HPLC 172
PP-12 method
T.Naga Raju1, D.Ravi Kumar1 and D.Ramachandran*
Temperature dependent study of thermophysical properties of binary mixtures of NVP with 172
PP-13 various imidazolium based ionic liquids
V.Visalakshammaa, T.S.Krishnab,* D.Ramachandranc
Synthesis of bismuth molybdate via Co-Precipitation method at different pH Condition and 173
evaluation of catalytic activity for degradation of methylene blue dye under visible light
PP-14
irradiation
B.Lavakusa1*, K.Basavaiah2, D.Srinivasarao3
Determination Of Genotoxic Impurity Hydrazine In Ursodeoxychloic Acid Using Rp-Lc 174
PP-15
Dr. D. Ramachandran* Dr.V.Siva Ramakrishna
Fluorescent Chemosensor for Various Metal Ions 175
PP-16
Eswar Pagadala,Shiva Krishna Loke,Ravi K. Kottalanka*and V. Srinivasadesikan*
Molecular interactions in binary mixtures containing n-butyl acetate with aromatic ketones at 176
various temperatures
PP-17
A venkatesh1, m. Radha sirija2, b. Hima bindu3, g.sireesha4, D. Ani manjeera5,
d.ramachandran*
Design and Synthesis of Difuran based fluorescent chemo-sensor for selective detection of 176
PP-18 Fe+3 ions
Shiva Krishna Loke, Eswar Pagadala, Ravi K. Kottalanka*and V. Srinivasadesikan
New Rp-Hplc Method For The Simultaneous Estimation Of Artemether And Lumefantrine 177
PP-19 In Combination Drug Products
Meka Srinivasa Rao* & Dr. K. Rambabu┼
Estimation Of Piracetam And Citicoline In Tablets By Rp-Hplc Method 178
PP-20
Validated Reversed-Phase-High Performance Liquid Chromatographic Assay Method For 179
PP-21 Furosemide And Spironolactone In Generic Combination Drugs
P.Sivannarayana* & Dr. K. Rambabu┼
Volumetric, acoustic and spectroscopic study of Diethylene 179
PP-22 Triamime-Amino Ethyl Ethanolamine with Alcohols
Dr. D.Ramachandran, Mrs. Shaik Gousia
Thermodynamic, Transport And Aucostic Properties Of Butyl Acetate With Some Alcohols 180
PP-23 At Different Temperatures
U.Vidyamani, Dr.D.Ramachandran
Validation Of A Head Space Gas Chromatographic Method For The Determination Of 180
PP-24 Ethylene Oxide Content In Dipyridamole Api
Sagi.Sambamurthy Raju, Gachakayala.Naresh Babu, Dunna.Sivaram
Development And Validation Of Rp-Lc Method For Aripiprazole In Pharmaceutical 181
Formulations
PP-25
Dr. D. Ravi Kumar1, Dr. V. Siva Ramakrishna2, T.Benjamin3,
T. Naga Raju1 and Dr. D. Ramachandran2
Development And Validation Of Simultaneous Estimation Of Glycopyrrolate And 182
Formoterol Fumarate In It’s Bulk And Pharmaceutical Rota Caps Dosage Form By Using
PP-26
Rp-Uplc
T.Benjamin1* , G.Rahul 2, Dr.Ch.Rajyalakshmi3 and Dr. D. Ramachandran4.
Study Of Molecular & Thermodynamic Parameters Of Binary Liquid Mixtures By Using 183
PP-27 Ultrasonics
* D. Ramachandran1, D.Nagendra2
Development and Validation of a new RP – HPLC method for estimation of Dapagliflozin 183
PP-28
Dr. P. Srinivasa Rao, Mr. R. Prasanna Babu
Simultaneous determination of Diclofenac Sodium and Tolperisone HCl in marketed tablets 184
PP-29 by RP-HPLC method
T.Naga Raju1, D.Ravi Kumar1, S.V.M.Vardhan2 and D.Ramachandran*
Selective separation, Detection of Daunorubicin and Cytarabine Mass spectral 185
PP-30 characterization of degradants by LC-MS/MS/QTOF
Thermodynamic parameters of binary liquid mixtures of chloroform and o- substituted 185
PP-31 aniline
K. Naveen kumara, K. Sambasiva Raob
Influence on chlorine atom on thermodynamic and transport properties of binary liquid 186
PP-32 mixtures at various temperatures (Halo-hydrocarbons and 3-methoxyaniline)
P. Nagababua, M. Gowrisankarb*
Convergent synthesis of aliphatic and aromatic thioureas 186
PP-33
Venkata Bhavanarushi Pendema, Nannapaneni Madhavi*b
Binary mixtures of 2,6-dimethyl cyclohexanone with various functional groups 187
PP-34 (m-cresol, p-cresol and ortho-chlorophenol)
Shaik Parveen Sulthanaa, M. Gowrisankarb*
Thermodynamic parameters of binary liquid mixtures of ortho-nitro toluene with various 188
PP-35 functional groups (2-ethyl-1-hexanol, 3-methylaniline, and 2,6-dimethylcyclohexanone)
D.Rahula, D. Ramachandranb,*,
Molecular Interactions In Binary Mixtures Containing Halo-Substituted Carbonyl Compound 188
PP-36 + Alkoxy Alkanols At Various Temperatures
M. Radha Sirija, P. Bharath, D. Ramachandran*
Pharmacological Studies of Cobalt Oxide Nanoparticles 189
PP-37
VeronicaDeekala, Jyothsna Pragathi Yazala, Anitha Kowthalam, RameshrajuRudraraju
Synthesis and Characterization of Silvernanoparticles Using leaf extract of the plant 189
PP-38 Alternanthera Brasiliana
Kollimarla Siri, G. Sravani, K. Preeni Praneetha and K. PrasadaRao*
Thermo physical and spectroscopic properties of binary liquid systems of 2-methylproponic 190
acid with various functional groups
PP-39
(2-ethyl-1-hexanol, 2-methylaniline, 2-methoxyaniline and 2-methylcyclohexanone)
P. VenkateswaraRaoa, M. Gowrisankarb, K. Ravindhranathc*
Estimation of strength of molecular interactions between 2,6‐dimethylcyclohexanone 191
PP-40 and chlorinated ethane / ethene components at various temperatures
K. Srinivasa Raoa, M. Gowrisankarb*
Liquid Chromatographic Estimation of Mianserin 192
PP-41
Giri Prasad Gorumutchu1 and Venkata Nadh Ratnakaram 2*
Ultra performanance Reverse Phase Liquid Chromatographic Method for the Quantification 192
PP-42 of 2,4-Difluorobenzoic acid in Elvitegravir in Drug product
Dr.D.Mogilireddy
Total Synthesis of Diplodialide C and D 193
PP-43
V.B.Ramanujan,Dr.Ch.N.Sai Pavan Kumar
Isolation and characterization of new pentacyclic triterpenoid 194
PP-44 from Caralluma attenuata root
G.Jayalakshmi, Prof. V. Anuradha*
Pharmacological study of Antidepressant Activity of Plocama pendula root extract 194
PP-45
Dr. K. Uma Maheswar1*, Dr. KRS Prasad2, Chaitanya Darapureddy2
Synthesis of 1,4-dihydropyridines derivatives 195
PP-46
Upendar Reddy Mandadapu1, Murali Krishna Cheepi2 & Mahesh Madala3
synthesis theoretical calculation, docking studies and anti microbial evaluation of 1-(3- 196
PP-47 fluoro-4-morpholinophenyl)-1h-tetrazol-5-amine derivatives.
B. S N Murthy1*, T. Ramana2, M. D. A. Saibabu3
Synthesis Ni(II) Complexes using N, O donor 197
PP-48 Schiff base ligands: Antioxidant, antimicrobial, DNA binding and cleavage studies
V. Sumalathaa,, K.Geetha bhavania, K. Niramala Jyothia
A Kinetic Study On The Oxidation Of Polyols By N-Haloagent 198
PP-49
K.V.S. Koteswara Rao1 and R. Venkata Nadh2*
Extractive ion pair complex formation For determination of febuxostat 198
PP-50 1 2*
Sudhir S Muvvala , Venkata Nadh Ratnakaram
Microwave assisted synthesis and anti-Inflammatory activity evaluation of Pyrazole 199
PP-51 derivatives
P. Naveen Kumar B. Ch. Naga Raju, K. Anjaneyulu and Dr. Ch.
Hplc Method Development And Validation For The Estimation Of Sulfomoxole In Bulk And 199
PP-52 Formulations
M.Kamala Karuna 1 and R.Venkata Nadh2*
Design an efficient method for the synthesis of 2-(1,3-diphenyl-1H-pyrazol-4- 200
PP-53 yl)benzo[d]thiazole and study their antimicrobial activity
B. Srinivas*1 and K. Saidulu2
Quality Characteristics of Some Edible Oils and Effect of Additives on Chemical Constants 200
PP-54
B. Jyothi1, Sk.Ameena2, A.Amulya3, N Madhavi4 *.
Biological Screening of Pyrimidines and Phenothiazines 201
PP-55 Boggavarapu Jyothi1, Thiyyagura Sravanthi2 ,Suryadevara Kalpana3 , Nannapaneni
Madhavi*
Synthesis and Characterization of Silver Nanoparticles Using Campsis grandifolia Plant 201
PP-56 Extract
B.Suresh, B. Bhaskar, E. Narsimha Reddy, P. Nagaraju and K. PrasadaRao*
Synthesis of chain transfer agent for reversible addition fragmentation chain transfer (RAFT) 202
PP-57 Polymerization technique.
B. S.V.S.M.ganesh1 , d ramachandran
Validated Method Development For The Assay Of Brexpiprazole In Pharmaceutical 203
PP-58 Formulations
Dr T N V S S Satyadev
Preparation of Lipbalm From the Peel of Hylocereus undatus 203
PP-59
K. Aravind lokesh, P. Asha Bhanu, G. Swapna, and Prof.B. Kesava Rao,*
Synthesis and Characterization of Silver Nanoparticles Using Polystichum munitum Plant 204
PP-60 Extract
G. V. V. Ramesh, D. Lakshmi Narayana, P. Manikanta Reddy and K. PrasadaRao*
Extraction and Saponification Of Oil From Avocado Fruit 204
PP-61
M.V.S. Manikanta, P Asha Bhanu and G Swapna, Prof.B. kesava Rao,*
Preliminary Chemical Investigation of Clitoria Ternatea 205
PP-62
G. Chandrika , P. Asha Bhanu, G. Swapna, and Prof.B. Kesava Rao,*
Phytochemical Investigation of Curcuma longa 205
PP-63
G. Kumudini Sri, P. Asha Bhanu, G. Swapna, and Prof.B. Kesava Rao,*
Isolation Of Lycopene From Solanum Lycopersicum 206
PP-64
M. Supriya, P Asha Bhanu, G Swapna and Prof.B. Kesava Rao,*
Simultaneous uv and first order derivative spectrophotometric estimation of imipenem and 206
PP-65 cilastatin in pharmaceutical dosage forms
Masimukku siva kishorea,b* and dittakavi rama chandrana
Synthesis of Some New 2-Benzyloxy-5-alkyne Substituted Pyrimidines through Sonogashira 207
PP-66 Coupling
O. Surendranatha Reddy, M. Baby Ramana, T. Vijaya Durga, Bollikolla Hari Babu*
Synthesis, Structural Characterization And Antimicrobial Activity Zinc Oxide Nanoparticles 207
PP-67 1
*T.Aparna, 2M.Krishna Murthy,2 K Surendra babu
Green Chemistry 208
PP-68
B.Sujatha, P.Neeradha, Dr B.Sridevi M.Sc ,Phd
Green synthesis of graphene-hydroxyapatite nanocomposites with improved mechanical 210
PP-69 properties for bone implant materials
Syed Akhil and Basavaiah Chandu
Design, Synthesis and Biological Evaluation of Sulphonamide Derivatives of Benzofuran- 211
PP-70 Imidazopyridines as Anticancer Agents
Sreenivasa Murthy, R. Sreenivasulu, and R. Ramesh Raju
Design, Synthesis and Anticancer Evaluation of 1,2,4-Thiadiazole linked Pyridone 212
PP-71 Derivatives
Neerada Perupogu, Syed Khasim Sharif, and Dittakavi Ramachandran
Purification Free and Chemoselective Acylation of Non-Nucleophilic 213
PP-72 N-Heterocycles Using Oxyma and benzotriazole activations
T. Bhagya Kumar1 & M. Manoranjani2
Design, Synthesis and Biological Evaluation of Chalcone Linked Thiazole-Imidazopyridine 214
Derivatives as Anticancer Agent
PP-73
Vellanki Ragha Suma, Reddymasu Sreenivasulu, Madala Subramanyam
Kuppili Ram Mohan Rao1*
Design, Synthesis and anticancer activities of 1,2,4-Thiadiazole 215
Linked 1,3,4-Thiadiazole-Carbazoles analogues
PP-74
Eeduri Ramya Devi1,2, Reddymasu Sreenivasulu2, Koya Prabhakara Rao1, Ratnakaram
Venkata Nadh3, Malladi Sireesha*1
Determination Of 3-Chloro-1,2-Propanediol In Iohexol Drug Substance Using High 216
PP-75 Performance Liquid Chromatography With Refractive Index Detector
N. Sanni Babu1, G. Sailaja2, M. Santha Kumari3, B. Hari Babu1
A new steroid isolated from Leucas cephalotes 216
PP-76
K. Kavitha Priya*1; V. Anuradha1,2 ; G. Jayalakshmi1& S. Ratnakumari2
A novel pyridyl ester isolated from Leucas cephalotes 217
PP-77
Kavitha Priya Kummaraa, Anuradha Vejendlaa*
Colorimetric determination of bendamustine 218
PP-78
K. Kiran kumar1 and r.venkatanadh2*
Bioanalytical Lc-Ms/Ms Method Development And Validation Of Novel Antidiabetic Drugs 219
PP-79 In Rat Plasma And Its Application To Pharmacokinetic And Oral Bioavailability Studies
T.Subrahmanyam1, V. Anuradha*2, BSN.Murthy3, G.R.Satyanarayana4
Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous 220
PP-80 Estimation of Epalrestat and Pregabalin in Bulk and Tablet Dosage Form
Md. Shabana Sulthana1, G. Ratna Babu2
Synthesis And Characterization Of Phenanthroimidazoles 221
PP-81
Thota Ambika, Dr. V. Krishnan
Isolation and Biological activity of Nybomycin 222
PP-82
B. Meenakshi, A. Mallikarjuna rao, Dr. D. Ramachandran*.
Synthesis and Anticancer Evaluation of 1, 2, 4-Thiadiazole bearing combretastatin-A4 223
PP-83 Derivatives as Anticancer Agents
S. Premalatha, G. and D. Ramachandran*
Evaluation of flavone-8-acrylamide derivatives as Multifunctional anti-Alzheimer agents and 223
their Mechanism of Action with Acetylcholinesterase
PP-84
K. Yelamanda Rao1, S. Jeelan Basha1, P. Mohan, Z. Raveendra Babu1, K. Mounika2, S.
Rajagopal2, A. G. Damu1*
“Synthesis of Arylcyanamides, Aryl Tetrazole Amines, Benzothiazoles and Benzoxazoles by 224
PP-85 Copper Catalyzed Desulfurization and C-N Cross Coupling Strategies”
S N Murthy Boddapati1,2, Hari Babu Bollikolla1⃰
Preliminary Chemical Investigation Of Piper Betel Leaves. 225
PP-86
Karanam Santoshi,P Asha Bhanu and G Swapna, Prof.B. kesava Rao,*
Design, Synthesis and Anticancer Evaluation of 1,2,3-Triazole Likned Thiazole-1,2- 225
PP-87 Isoxazole Derivatives as Anticancer Agents
Thota Yakanthama, Reddymasu Sreenivasulub, and Rudraraju Ramesh Rajua*
PHARMACEUTICAL POSTER PRESENTATIONS
PP-1 Evaluation of Antibacterial activity of Silver Nano-particles on Banana Blossoms 227
B.Nausheen
PP-2 In-Silico Prediction And Molecular Docking Studies As Novel Targets For Various Drugs 227
Thota Imran Basha
PP-3 Synthesis and evaluation of isatins showing Anti microbial activity. 228
Bandapalle Malli
PP-4 Green project is both conversation and education intiative focused on sustainable use of land. 228
One of the goals of green chemistry is to prevent pollution after it has occured.
D.G.Manasa
PP-5 poisonous chemicals emitted in factories are dangerous and exposes us to various elements 229
that cause cancer
S.vanaja
PP-6 Bioanalytical Techniques For Determination Of Drug And It’s Metabolite 229
M.Balakrishna, Ch.Suneetha and K. Padmalatha
PP-7 Combinatorial Chemistry In Drug Discovery: A Review 230
DPrasanna , A.Hari chandana and K.Padmalatha
PP-8 The Next Level In Chemical Space Navigation: Going Far Beyond Enumerable Compound 230
Libraries
D.Prasanna, T.Deepika and K. Padmalatha
PP-9 Biomarkers In Acute Myocardial Infarction: Current Perspectives 231
M. Bala Krishna, Divya Sree. Ch and K. Padmalata
PP-10 Herbal Mediated Silver Nanoparticles: A New Horizon Of Antineoplastic Drug Delivery 231
System
S. Venkateswara Rao, M. Anvitha*, Padmalatha Kantamneni
PP-11 Organ –On- A- Chip Technology: A Review 232
D.Prasanna, O.KrupaSanthi and K.Padmalatha
PP-12 Role Of Natural Products In Drug Discovery 232
Jayarami Reddy A, Rajyalakshmi K and K.Padmalatha
PP-13 Principles And Applications Of Liquid Chromatography-Mass Spectroscopy 233
M. Vamsi Krishna, N.Sailaja and K.Padmalatha
PP-14 Green Chemistry And Its Multidimensonal Impacts: A Review 233
D.Prasanna, R .Satyaveni and K. Padmalatha
PP-15 Nanomedicine In Diabetes Mellitus- A Review 234
A Jayarami reddy, P. SINDHU and K. Padmalata
PP-16 Role Of Telomeres In Cancer And Ageing 234
Author: T. Maniratnam
BIOLOGICAL POSTER PRESENTATIONS
effect of salt stress on foxtail millet (setaria italica l.beauv) during seed germination 235
PP-1
D. Rama Rao and Y.R.K.V.Tirupati Rao*
Extraction, Identification And Estimation Of Thiamine In Whole Grains By HPLC 235
PP-2
Dr.K.Suneetha and Hari chandana
Identification And Estimation Of Flavonoids In Aloevera By TLC and HPLC 236
PP-3
Dr.K.Suneetha, R.Sushma and M.Sri Lakshmi Durga
Antimicrobial and antioxidant activity of phytochemical constituents of Zornia diphylla L. 237
PP-4
L. Mutyala Naidu
Cyanotoxinns and Algal-Blooms 237
PP-5
M. Rajitha, Sudharani Chalapala and S. Anwar
The green synthesis, characterization, and evaluation of the biological activities of silver 238
PP-6 nanoparticles
M. Sandhya Rani, B. Pavani Bai, G. Gowthami, Sudhakar Podha*.
β-Sitosterol (BS) Biological Activity 238
PP-7
R. Gayathri, Sudharani Chalapala and S. Anwar
Environmental Biodiversity Conservation 239
PP-8
S. Rehana, Sudharani Chalapala and S. Anwar
Marine Fungi - A Source of Bioactive Metabolites 240
PP-9
S. Shabana1, k. Rajya Lakshmi1 and A. Krishna Satya1*
Comparative studies on the Biochemical composition of male and female mud crabs, Scylla 240
PP-10 serrata and S. olivacea
Parvathi, D. and P. Padmavathi
Effects Of Pesticidal Pollution On Environment 241
PP-11
K. Ravibabu, G.Rajeswari & Dr. V. Venkata Rathnamma
Nutritional Genomics: An Overview 242
PP-12
S. Sundar, S. Geetha and K.Padmalatha
AminoAcids for plants 242
PP-13
M.Kala, Sudharani Chalapala and S. Anwar
Reducing the incidence of insulin resistance 243
PP-14 By the mutagnesis of wheat
S. Sundar, SupriyaMareedu and K.Padmalatha
Fungal pathogen associated with murraya koenigii l. 244
PP-15 1
Soumya K.R, & Anjali M2
Isolation and purification of bioactive form microbes to establish their antagonistic activity 244
against fungal pathogens on Soya bean.
PP-16
Qasim Turki Kadhim, Merakanapalli Kishore Babu, Kunchala Rajyalakashmi, DSD Suman
Joshi, Subramaniam Gopalakrishnan, Vadlamudi Srinivas, Alapati Krishna Satya *
PP-17 In Silico studies in identification of MAP4K4 Inhibitors 245
Dorka Vijayakumari Beri1,2, Krupanidhi Srirama1
Studies on Improvement of Efficacy of Lactobacillus acidophilus for Industrial Productivity 246
PP-18
J. Someswari Devi, G. Naveena, K. Jamalaiah, Dr. Alapati Krishna Satya*
Study Of Municipal Solid Wastage Management Scenario Of Tenali City 247
PP-19
B. Satish Babu1, G. Vijaya Kumar 2, Dr. P. Brahmaji Rao3
1st International Conference on Recent Advances in Chemical, Pharmaceutical and Biological Sciences
(RACPABS - 2020)
KEYNOTE ADDRESS
Molecules with Celebrity Status
S Chandrasekhar
CSIR-Indian Institute of Chemical Technology, Hyderabad
srivaric@iict.res.in
Nature, through biosynthetic pathways, generates simple to architecturally very

complex carbon frameworks, which have medicinal value to cure diseases. Despite great
progress made in the health care, the mortality rate globally is still at a very high rate. There
are several diseases for which the root cause needs to be understood well before discovering
drugs. These include Cancer, CNS and infection disorders. Natural Products have been a
source of inspiring starting molecules in early drug discovery. Several natural products and
synthetic molecules have attained the status of “True Celebrity”. Our research group has
been engaged in both total synthesis of bioactive natural products and their synthetic analogs
towards identifying newer drug leads.
The lecture will highlight our contributions in the synthesis of natural products, which
have relevance in identifying leads in Cancer, CNS and infection disorders. Some
background on the molecules, which have changed the world with wonder properties, will
also be discussed.
Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 1

(RACPABS - 2020)
INVITED TALKS
IT1: Phytonanotechnology – Anti-diabetic drugs and Possible Mechanisms
Pasupuleti Visweswara Rao

Department of Biomedical Sciences and Therapeutics, Faculty of Medicine and Health
Sciences, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia
Nanotechnology and nanoparticle research plays an important role in the human life
in terms of technology and advancements. Herbs or plants have become vital in everyday life
activities. Nanotechnology excels not only in the field of engineering, but also various other
fields such as architecture, food, agriculture and also medicine. In the field of medicine, the
advancements of nanotechnology serves in various types of diseases including diabetes.
Diabetes is one of the major global burdens which needs more attention at the present
scenario. Various types of anti-diabetic drugs are in existence but with one or other side
effects. Nanoparticle synthesis or nano advances is one of the option to treat several diseases
including diabetes. Biological synthesis or bio-fabrication of nanoparticles from medicinal
plants play a vital role in the field of nanotechnology with biomedical applications. Herbs or
medicinal plants usually contain polyphenols and flavonoids which ameliorates the damage
in the cells with antioxidant capacity. Nanoparticles synthesized through biological methods
are potentially strong against the diseases including diabetes and also safer when compared to
the chemically synthesized nanoparticles. Insulin replacement therapies, super paramagnetic
iron oxide nanoparticles (SPIONS), handheld glucometers, continuous glucose monitors
(CGMS), Glucose oxidase, glucose-binding proteins and glucose-binding small molecules,
Encapsulation of β-cells within semi-permeable polymer matrices may be the options with
the advancements of nanotechnology. Basic mechanism(s) of action related to diabetes and
management are in existence. However, the exploring the possibilities towards delving the
clear anti diabetic mechanisms of actions with the nanotechnology and nanoparticle research
would be advantageous to the pharmaceutical industries as well as mankind.

(RACPABS - 2020)
IT2: Friccohesity and Survismeter: Theory and Applications in Areas of

Chemical, Pharmaceutical, and Biological Sciences
Man Singh
School of Chemical Sciences, Central University of Gujarat, Sector 30 Gandhinagar,
Email: mansingh50@hotmail.com.
Chemical, pharmaceutical, and biological sciences are centred around the functional
molecules through SAR (structural activity relationship) and FCR (friccohesity coefficient
relationship) materialized through potential medium as solvent. SAR and FCR indicate
applicability of molecules for specific chemical activities and processes. The molecules orient
and optimize through electronic develop electrostatic dipoles (Zwitter ions, polar peptide
bonds), induced dipole with large sized atoms like iodine, pi conjugation, electrostatic
delocalization, charge alignments; atomic positions at o, m, p positions of benzene ring,
mutual protonation. These structural constituents tune and modulate the chemical,
pharmaceutical and biological activities to be rendered for specific applications. Flavonoids
like curcumine, quercetin seem efficient for releasing proton to scavenge free radical like
DPPH molecules. The above-mentioned theme develops a vacancy to elaborate the activities
of the molecules in specific medium in vitro. These activities are direct functions of structural
constituents of molecules that develop cohesive energy (surface tension), shear stress
(viscosity), surface area, dipole moment, activation energy, shear stress, interfacial tension,
and wettability. These functions develop specific interactions and act as indicators to overall
behaviour of molecules/biomolecules in specific domain. The product of cohesive forces and
shear stress or frictional force is noted as friccohesity. The physicochemical profile of
molecules is a useful database for efficient and selective activities so their quantitative
measurements are prerequisite without transferring them to different probes/instrumental
techniques. The survismeter together measures the said physicochemical properties that
prevent redox, contamination, evaporation, coagulation, and flocculation. In regard to these, a
liquid mixture is made by a mutual contribution of frictional and cohesive forces as per
interacting activities of each constituent of a molecule and their integrated profiles determine
overall molecular abilities. In the lecture the hardcore activities of few surfactants, aromatic
compounds, ionic liquids, flavonoids which are obtained using survismeter will be discussed.

(RACPABS - 2020)
IT3: Borneon Natural Products: Anti-Inflammation/Anti-Cancer Activities

and their Mechanism of Action
Charles Santhanaraju Vairappan

Laboratory of Natural Products Chemistry, Institute for Tropical Biology and Conservation,
Universiti Malaysia Sabah, 88450, Kota Kinabalu, Sabah, Malaysia
Borneo, known as a biodiversity hotspot in the South East Asia, is rich with high
diversity of terrestrial and marine flora/fauna. As part of our ongoing research, we have
continually reported the diversity of secondary metabolites in plants and animals collected
from Borneo. A total of 1000 compounds were isolated and their diversity was studies
interms of chemical structure, source and geographical location. These compounds were
subjected to biological activity such as anti-inflammation and anti-cancer. Inflammation is a
powerful innate immune system defense that is an orchestrated maneuver designed to
eliminate cellular treats. Chronic inflammatory response plays an important role in cancer
development and resistance to chemotherapy. Mechanism of action responsible for
inflammatory diseases are not fully understood and there is a need to better understand their
mechanism. Molecular mediators that regulate inflammation and cancer are promising targets
for preventing and treating these diseases. In this study, we have identified novel secondary
metabolites that showed potent activities in both the assays. Compounds were isolated and
their structures elucidated based on spectroscopic data. Their inflammatory potential and
mechanism of action was evaluated using RAW 264.7 macrophages, their PGE2, TNF- , IL-
1 , IL-6, iNOS, and COX2, were evaluated. Cancer cell bioassay was concluded using HL60
and MCF 7 cell lines, with control normal cells. Apoptosis mechanism was evaluated using
Sub-G1 proportion, microscopic technique, Bax, Bcl-xl, Cleaved Capcase 3 and -actin. In
addition, we also investigated the microarray gene expression of these compounds on the
clinical cancer cells to identify the genes that were up/down regulated during this study.

(RACPABS - 2020)
IT4: WETLAND DEGRADATION AND ITS IMPACTS ON NATURAL

RESOURCES IN ETHIOPIA- A REVIEW
Lakew Wondimu Abachiri

Department of Biology, Ambo University, P.O.Box. 19, Ambo, Ethiopia.
Email: lakewwondimu84@gmail.com
P. Natarajan and Kassye Balkew
Department of Biology, Hawassa University, Hawasa, Ethiopia.
Wetlands are one of the most productive aquatic ecosystems offer great scope for rich
biodiversity. It serves as nursery and breeding grounds for a large number of commercially
important food fishes. The interspace between wetlands and terrestrial region is unique as it
harbors lifeforms which are tolerant to various environmental changes. It is on the forefront
of the developmental agenda because of their ecological, socio cultural and economic
benefits. They provide direct and indirect services to mankind. The various ecosystem
services of wetlands include regulating water flows, nutrient cycling, storing water,
recharging and supplying ground water, storm protection, flood mitigation, shoreline
stabilization, erosion control, trapping of nutrients, soil formation, controlling floods and
droughts, sewage water purification through natural process of oxidation, radiation and
biological breakdown of the organic matter and water purification. Wetland assumes
significance in climate change mitigation as they serve as sink of greenhouse gases,
especially carbon di oxide through carbon sequestration. These wetlands are now under
serious threat and are degrading rapidly all over the world due to various factors.
Ethiopia is a landlocked country, and although Ethiopia is described as water tower in
Africa, the rich water resources available in the form of large, lakes, lengthy rivers, reservoirs
and small water bodies with a water expanse of about 7400km2 are now under heavy
pressure due to multiple factors. The present paper reviews the various factors that are
responsible for the degradation of such water bodies. The various aspects described in this
paper include, deforestation, soil erosion and sedimentation, urbanization and industrial
growth, wetland cultivation, grazing, unregulated resource exploitation, weed infestation and
eutrophication, exotic species introduction, encroachment and reclamation. Also the paper
suggests some measures to overcome such issues with a view to enhance the life and life
management systems of mankind.
Key words: Wetlands, water resources, wetland degradation, resource depletion, remedial
measures.

(RACPABS - 2020)
IT5: Exploring the Prevalence and Risk Factors of Prediabetes in Adult

Population of West Shoa Zone, Oromia region, Ethiopia – A Community
Based Study
Dr. R.Vinodhini1*and Dr. Gudeta Imana2

1. Department of Biochemistry, College of Medicine and Health Sciences, Ambo University,
Ambo, Ethiopia.
2. Department of Internal Medicine, College of Medicine and Health Sciences, Ambo University,
Ambo, Ethiopia. *Email: vinobchem@gmail.com, sivaniswetha@yahoo.com
Diabetes mellitus (T2DM) is a state of chronic hyperglycemia due to complete or

comparative insulin deficiency that leads to glucose accumulation in the blood. Long term
complications include diabetic ketoacidosis, related with dysfunction and failure of different
body-organs, especially the kidneys, eyes, heart, nerves and blood. T2DM represents a
medical and socio-economic crisis across the globe and its burden continued to increase in
low and middle income countries (LMIC’s). According to IDF atlas 2017, approximately 451
million (8.8%) adults worldwide are expected to have diabetes, and it is estimated to reach
693 million (9.9%) by the year 2045. Research evidences reported that life style intervention
programs conducted in high income countries offer global guidelines which had wide
variations and not feasible in the health system performance of LMIC’s. T2DM is
asymptomatic and hence effective approaches are required to prevent the complications and
progression of this disease. The aim of our study was to track the risk groups at prediabetic
stage on adult population living in west shoa zone. A cross sectional study design was
conducted on participants visited the OPD services of Ambo general hospital (AGH), Ambo
University Referral hospital (AURH), Guder district hospital (GDH) and Gedo district
hospital (GeDH). The targeted participants were adults under the age group of 18- 65+ years.
We included self-administered questionnaires; anthropometric data and blood biochemistry
profile to identify the target population. A total of 480 respondents arrived at the baseline
sample in which 58 subjects were excluded and the remaining 422 samples with normal
glucose tolerance (NGT) and impaired fasting glucose (IFG) were included as the study
subjects. All statistical analysis was carried out by IBM SPSS statistics 20.0 software. The
recorded p values are on the basis of two-sided tests with a statistical significance of p ≤ 0.05.
The results of our study concluded the prevalence as 47.1% in AGH, 44.9% in AURH, 46.6%
in GDH and 39.7% in GeDH respectively. The present study indicated a higher prevalence of
prediabetes and the effect of possible risk factors in the target population. Hence lifestyle
intervention program should be prioritized in the high risk groups to maintain the normal BP,
blood glucose, BMI and regular physical exercise. This strategy helps in the management of
prediabetes and controls a huge number of people from the risk of T2DM.
Key words: Prediabetes, Type II diabetes mellitus, intervention, blood glucose

(RACPABS - 2020)
IT6: Recent Approaches in Natural Products for New Drug Development
Dr. K. Suresh Babu

Principal Scientist
Centre for Natural Products & Traditional Knowledge
CSIR-Indian Institute of Chemical Technology,
Hyderabad-500 007, INDIA
Email: suresh@iict.res.in
The Indian subcontinent has a vast costal belt along with the wide range of forest
environment, which host large number of un explored plant/marine species. This diversity has
been the source of unique chemical compounds with the potential for industrial development
as pharmaceuticals, cosmetics, nutritional supplements, molecular probes, fine chemicals and
agrochemicals. In recent years, a significant number of novel metabolites with potent
pharmacological properties have been discovered from the natural sources including marine
and terrestrial plants. Recent move of society towards nature for the treatment of various
diseases where there is no satisfactory cure in modern medicine has diverted the attention of
natural/medicinal chemists and biologists to unravel their chemical characteristics and
biological activities together in order to define their therapeutic potential in the light of
modern pathobiological understandings. This move has led collectively to rediscover, design
and refine the therapeutic application of medicinal plants/marine sources.
During last eight years, we have studied several medicinal plants and marine
organisms guided by in vitro based bioassays to delineate the chemistry of natural products
responsible for biological activities. This effort has led to identify several potent multiple
active medicinal plants/marine sponges, their active fractions and synergistic molecular
compositions. We have identified particularly, several free radical scavengers, xanthine
oxidase and α-glucosidase inhibitory principles present in substantial yields. Presence of
multiple active phytochemicals in rich concentrations in some of the medicinal plants
therefore offers exciting opportunity for development of novel therapeutics and also provides
scientific justification for their use in traditional medicines. Therefore, biologically activity
based chemical characterization of these medicinal plants may provide scientific explanation
for their use in traditional medicines and also redesign and develop preparations for novel
therapeutic applications.

(RACPABS - 2020)
IT7: Exploring Synthetic Molecules and the Nano-Bio Platform as

Therapeutics Against Drug-Resistant Pathogenic Bacteria
Aiyagari Ramesh
Department of Biosciences and Bioengineering
Indian Institute of Technology Guwahati, Guwahati 781039.
The prevalence of drug-resistant pathogenic bacteria in conjunction with an acute

dearth of therapeutic antibiotics constitute a significant healthcare burden. This predicament
underpins the need of potent antibacterials that can act on profound cellular targets and are
unlikely to trigger resistance development. Our research is focussed on exploring the
chemistry-biology interface and generate antibacterials that hold therapeutic potential against
antibiotic-resistant pathogenic bacteria. Our endeavour is to use rationally designed synthetic
amphiphiles and small molecule ligands 1-6 and leverage their therapeutic prospect through
non-toxic nanocarriers. 7-11 We have also demonstrated the potential of natural antimicrobial
peptides such as bacteriocins produced by lactic acid bacteria (LAB) and probiotic LAB in
hindering pathogen adhesion onto extracellular matrix (ECM) and intestinal cells. 12-14 The
presentation will highlight the prospect of synthetic, medicinal and materials chemistry as a
promising framework to generate therapeutic materials with enhanced capabilities against
drug-resistant pathogenic bacteria.
References
[1] Goswami et al., Journal of Materials Chemistry B 2013, 1, 2612-2623.
[2] Thiyagarajan et al., Chemical Communications 2014, 50, 7434-7436.
[3] Goswami et al., Journal of Materials Chemistry B 2015, 3, 7068-7078.
[4] Sahareen et al., ChemBioChem 2018, 19, 1400-1408.
[5] Dey et al., Journal of Materials Chemistry B 2018, 6, 2116-2125.
[6] Kapoor et al., ChemBioChem 2017, 18, 1502-1509.
[7] Goswami et al., ACS Applied Materials & Interfaces 2014, 6, 16384-16394.
[8] Chauhan et al., ChemistrySelect 2018, 3, 2492-2497.
[9] Thiyagarajan et al., ChemNanoMat 2016, 2, 879-887.
[10] Thiyagarajan et al., ChemMedChem 2017, 12, 1125-1132.
[11] Mullick et al., ACS Applied Biomaterials 2019, 2, 2927-2936.
[12] Mukherjee and Ramesh, Journal of Medical Microbiology 2015, 64, 1514-1526.
[13] Mukherjee and Ramesh, Microbiology 2017, 163, 1822-1834.
[14] Mukherjee et al., ACS Applied Biomaterials 2019, 2, 3225-3233.

(RACPABS - 2020)
IT8: Synthesis, spectroscopic and electrical characteristics of alkali

sulphophosphate glasses mixed with some d and f block ions
P. Raghava Rao1, A. Siva Sesha Reddy2 and N. Veeraiah2*

2
Department of physics, Dr. M.R. Appa Rao PG Centre Krishna University,
Nuzvid-521 201, A.P., India
2
Department of physics, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur,
A.P., India Email: nvr8@rediffmail.com
Alkali sulphate mixed phosphate glasses are considered as appropriate materials for
the immobilization of radioactive waste. These glasses exhibit unique electrolytic properties
that make them suitable for several solid-state electrochemical devices such as high
temperature solid-state sodium–sulphur batteries, high density micro-batteries, sensors, smart
card and medical appliances etc. These glasses are also ideal hosts for rare earth ions that
emit luminescence in the visible and NIR regions. In this work, we have synthesized
Li2SO4/Na2SO4 -P2O5 glasses doped with different transition metal ions (viz., Fe, Ni and Cu).
The valance states of these ions and their coordination number in the glass have been
investigated using optical absorption, EPR, IR and Raman spectroscopy. Later, ac and dc
conductivity studies have been performed on these samples. The optimal concentration of
each ion for getting maximal electrical conductivity has been identified and reported. The
conductivity and the dipolar effects mechanisms were analyzed quantitatively as functions of
valence states and the concentrations of the dopant ions. The studies were also extended to
the luminescence features of some rare earth ions viz., Sm3+, Er3+ and Ho3+ ions in the visible
and NIR regions in these glasses and we have identified suitable chemical composition of the
glasses for achieving intense visible and NIR laser emission beams.

(RACPABS - 2020)
IT9: Synthesis of an antipsychotic drug substance Clozapine with a very

economic key starting material 2-chloro-benzoic acid
S. Venkat Rao1*
1
R&D center (A DSIR approved facility), SMS pharmaceuticals Ltd, Sy. No.189, 190,
Gagillapur (V), Hyderabad-500047, India.
Email: venkat@smspharma.com, svr.org@gmail.com
An economic, commercial manufacturing process for clozapine is reported by using

an alternative starting material known as 2-chlorobenzoic acid (9) to regular anthranilic acid.
The selection of starting material places an important role in producing non-polluted
commercial and potential active pharmaceutical ingredients. Being anthranilic acid is a
narcotic one, its production involves lot of pollution and toxic reagents; it is chosen 2-
chlorobenzioc acid and 4-chloro-1, 2-diamino benzene as precursors for Ullman reaction to
produce multi scale level Clozapine as per ICH guidelines.
Keywords: Synthesis, Clozapine (1), new precursor, 2-chlorobenzoic acid, ICH guidelines,
Ullman type reaction, commercial manufacturing.
Graphical abstract / Route of Synthesis

CH3
N
NH2 HO O
Cl NO2 N
NO2 H Cl N
K2CO3, CuI, DMF N 2, d
OH c
4 3
N
9 O 8 Cl b H
Cl 5 1
References:
1. F. Hunziker, E. Fischer and J. Schmitz, 11-Amino-5h-Dibenzo [B, E]-1, 4-Diazepine
.10. Mitteilung Uber Siebengliedrige Heterocyclic, Helv. Chim. Acta, 1967, 50(6),
1588–1599. DOI: 10.1002/hlca.19670500618.
2. Tomas De Paulis Daniel A. Davis Howard E. Smith David H. Malarek Arnold A.
Liebman, Journal of Labelled Compounds and Radiopharmaceuticals, 1988, 25(9),
1027-1033. DOI: 10.1002/jlcr.2580250916
3. Michael J. McLeish Benny Capuano Edward J.Lloyd, Journal of Analytical Profiles of
Drug Substances and Excipients Volume 22, 1993, Pages 145-184. DOI:
10.1016/S0099-5428(08)60241-60245.

(RACPABS - 2020)
IT10: Design and Synthesis of Novel Bio-active Organophosphorus

Compounds
Suresh Reddy Cirandur

Department of Chemistry, Sri Venkateswara University, Tirupati- 517 502, A.P., India
E-mail: csrsvu@gmail.com
In the last decades, organophosphorus compounds (OPC’s) have been widely

employed as substrates in both natural products and synthetic applications due to their ease in
reactivity with organic groups usually bonded directly to phosphorus or bonded through a
heteroatom. These organic groups exert significant effect on the biocidal activity of the
OPC’s. Due to their unique physiochemical properties and potent bio activities, they are used
as medicines in agricultural, horticultural veterinary and human diseases. They have been
also used as potent enzyme inhibitors, antimicrobial, antitumor, antioxidant, antiviral,
antidiabetic and pesticidal agents [1].
Pharmacophore hybrid approach is one of the most effective methods in medicinal
and pharmaceutical chemistry to yield new drugs. It includes the fusion of two or more
pharmacophoric groups in a single molecule to attain synergetic outcome. It also affords an
improved selectivity profile with enriched pharmacokinetic and pharmacodynamic
boundaries, decrease of antagonistic side effects, having dual or numerous modes of action,
and lesser drug-drug interactions. This method has been successfully carried out in our
research through C-P bond formation by the incorporation of a phosphonate motif into
various biologically potent acyclic, cyclic and heterocyclic moieties and has had very
remarkable results of the target compounds having multifactorial activities such as anticancer
& antioxidant [2], antimicrobial [3], antidiabetic [4], pesticidal [5] and so on.
References
[1] S. Demkowicz, J. Rachon, M. Da´sko and W. Kozak, RSC Adv. 2016, 6, 7101-7112.
[2] G. Mohan, S. Santhisudha; S. Murali,; N.B. Reddy, G. Sravya, V. Z. Grigory and C.S.
Reddy, Res. Chem. Int. 2018, 44, 3475-3491.
[3] G. Mohan, S. Santhisudha, K.M.K.; Reddy, N.V. Reddy,; T. Vijaya,; C.S. Reddy,
Heteroatom Chem. 2016, 27, 269-278
[4] S.K. Balam, S.K. Krishnammagari, S.J. Harinath, S.P. Sthanikam, S.S. Chereddy, V.R.
Pasupuleti,; N.K. Yellapu, V.G.R. Peddiahgari, S.R. Cirandur, Med. Chem. Res. 2015,
24, 1119-1135.
[5] G.C.S. Reddy, C.R. Rani, M.V. Reddy, C.S. Reddy, Arch. Pharm. Chem. Life Sci.
2014,
347, 819-824.

(RACPABS - 2020)
IT11: MULTI-FUNCTIONAL LIPID-DYE- Fe@CNPs CONJUGATES

FOR BIOMEDICAL APPLICATIONS
Mandava V. Basaveswara Rao

Dean, Faculty of Sciences, Krishna University, Machilipatnam, A.P, India
vbrmandava@yahoo.com, professormandava@gmail.com
Treatment of cancer is difficult due to invasive and recurrence nature of the cancer
cells. Surgery in most of the cases is not fully successful unless supported by Chemotherapy
which is based on highly corrosive drugs such as Taxol, DOX Doxorubicin (Dox) and
Cisplatin. long term usage of drugs, MDR problem arises. Radiotherapy uses a harmful
ionizing radiation, which has a higher risk of damage to normal tissues. Photodynamic
therapy is a form of phototherapy involving light and a photosensitizing chemical substance,
used in conjunction with molecular oxygen to elicit cell death phototoxicity. PDT has proven
ability to kill cancer cells. The idea of developing a multi-functional nanoparticle is that it is a
single nanoparticle formulation capable of delivering, targeting, diagnosing and treating the
cancer diseases. Conventional PDT limits the use of photo sensitizers absorb only uv and
visible wavelengths. They also lack water solubilities. To overcome these problems, new
photosensitizers need to be developed so that they can absorb near infra red (NIR)
wavelengths and facilitate deep tissue penetration. The near-infrared (NIR) window (also
known as optical window or therapeutic window) defines the range of wavelengths from 650
to 1350 nanometre (nm) where light has its maximum depth of penetration in tissue. Within
the NIR window, scattering is the most dominant light-tissue interaction, and therefore the
propagating light becomes diffused rapidly. Lipofectamine is a common transfection reagent
used to increase the transfection efficiency of RNA or plasmid DNA into in vitro cell
cultures by lipofection. Lipofectamine contains lipid subunits that can form liposomes in an
aqueous environment, which entrap the transfection payload, upon mixing with iron
encapsulated carbon nano particles Fe@CNPs HeLa (human cervical cancer cells) were fed
with these nanoparticles for 2 hrs and then washed with PBS The cells were then irradiated
with NIR Halogen lamp and then the cell viabilities were estimated using MTT assay . The
nanoparticles internalized HeLa cells were measured for subsequent Reactive oxygen species
generation (for the investigation of molecular mechanism). As a function of concentration,
the ROS levels were increased upon photo irradiation. Singlet O2 is an electronically excited
state of molecular oxygen (O2), which is less stable than triplet oxygen.The energy difference
between ground state and singlet oxygen is 94.3 kJ / mole. It is a highly important reactive
oxygen species used in photodynamic cancer treatments (PDT). In gaseous phase, singlet O2
is extremely long-lived (~ 72 min). Interaction with solvents reduces the lifetime from
microsecond to nanosecond. Singlet O2 is a very important reactive oxygen species (ROS)
involved in PDT. It directly reacts with lipid membranes to undergo lipid peroxidation and

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leads to the damage of proteins and nucleic acids via Type II photo oxidative mechanism.
Conventionally many organic photosensitizers can generate singlet O2. Some of them are
clinically available. Eg: Photofrin. Conventional dyes suffer from photobleaching. Metal
NPs are more resistant to enzymatic and photodegradation problems. Metal NPs possess
ultra-high extinction coefficients than conventional dyes. The idea of developing
multifunctional nano particle that Single nanoparticle formulation capable to deliver, target
and treat diseases and is highly required for personalized medicine. phototherapy involving
light and a photosensitizing chemical substance, used in conjunction with molecular oxygen
to elicit cell death (photo-toxicity). PDT has proven ability to kill cancer cells. Lower
toxicity, biocompatibility and tunable absorption makes them as an attractive candidate for
PDT treatments.
IT12: Mutagenesis aids in studying pathogenesis and vaccine development

against Ehrlichia and Anaplasma species pathogens impacting human and
animal health
Roman R. Ganta
MSc, PhD, Professor and Director, Center of Excellence for Vector-Borne Diseases, College
of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA
Ehrlichia chaffeensis is an obligate intracellular tick-borne bacterium that causes

human monocytic ehrlichiosis (HME). Obligate intracellular bacteria (obligates) belonging
to the order Rickettsiales, including Ehrlichia and Anaplasma species, cause serious diseases
in people worldwide and in many animal species. Lack of an efficient mutagenesis methods,
particularly targeted mutagenesis in obligates causes a major impediment in understanding
microbial pathogenesis. We have succeeded in generating random mutations in E.
chaffeensis, a method that works well in creating mutations at random. Mutations in specific
genes of interest followed by complementation remained problematic for obligates and is a
highly sought-after goal. We therefore developed carefully thought-out methods to generate
stable targeted mutations by allelic exchange in E. chaffeensis. Targeted mutations in E.
chaffeensis were created to both disrupt several genes, and also to restore the function of a
mutated gene leading to the restoration of transcription and protein expression from an
inactivated gene. Subsequently, the methods are adopted as valuable in generating modified
live attenuated vaccines and in defining pathogenesis. The methods are broadly applicable
for several pathogens belonging to the genera Ehrlichia and Anaplasma.

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IT13: Nanoscience in Interventional oncology: Opportunities

& Challenges
Venkateswara Gogineni
Department of Radiology, Medical College of Wisconsin, Milwaukee, WI
Recent developments in nanoscience started to answer outstanding clinical questions

in interventional oncology field. With marked prominence of interventional oncology and its
projected market rise to USD 2.9 billion by 2024 from USD 2.0 billion in 2019, nanoscience
is being explored for the improvement of all facets of interventional oncology including, but
not limited to, diagnostic techniques, image guided ablation, high intensity focused
ultrasound, embolization and palliative techniques. A combination of the nanoscience with
interventional oncology facilitate targeted delivery of therapeutics, achieve locally high
concentrations of drugs and carry hydrophobic or highly toxic drugs to the site of action
effectively and safely. While the opportunities are enormous, further preclinical and clinical
explorations are necessary to make it a reality. In this context, I will present some of the most
recent preclinical studies we are conducting in our laboratory to develop novel theranostics
and to design trigger responsive cargo loaded nanomaterial that are compatible for
interventional oncology needs.
IT14: Green chemistry: Examples and perspectives for future
Satyaban Jena
Director–Academic, KIIT Deemed To Be University, Bhubaneswar, Odisha, India,
E-mail:sjena54@yahoo.com
The concept of green chemistry has appeared during a special programme launched
by the United States Environmental protection Agency (USEPA) by P. T. Anastas. The name
Green Chemistry indicates that the technology which imbedded, environmental friendly
and cost effective for the utilization of resources and minimize or even eliminate the
formation of harmful bi-products or toxic products[1]. Green chemistry is commonly
presented by a set of twelve principles. The principles comprise instructions for professional
chemists to execute new chemical compounds using environmentally benign process
technologies. The first principle describes the basic idea to protect the environmental
pollution. The remaining principles are focused on such issues as atom economy, formation
of toxic substances, solvent free media. These principles are also emphasizing the application
of renewable raw materials for synthesis of chemicals fuels using low energy consumption
[2].

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Presently, most of the chemicals are synthesized from non-renewable petroleum

feedstocks that are being depleted rapidly. However, in nature vast amount of renewable
materials like biomass which contains cellulose, hemicelluloses, lignin carbohydrates, amino
acids, and triglcerides etc are present to synthesize organic products [3]. The major
impediment to use biomass as a feedstock, it needs a novel chemistry to transform selectively
and efficiently in its natural state without further any modification. Combustion of fuels
obtained from renewable feedstocks is more preferable than combustion of fossil fuels from
depleting finite resources.
Physical and chemical reaction parameters play the most important role for the
synthesis of chemicals, fuels and energies. Development of catalysts like heterogeneous
catalyst, biocatalyst, electro-catalyst, photo catalysts etc towards high product selectivities,
reactivities, and stability are also an important characteristics of green chemistry. Reaction
conditions which can potentially provide for the benefits of chemical syntheses in terms of
resource and energy efficiency, product selectivity, operational simplicity health and
environmental safety. Solvents are auxiliary materials used in chemical synthesis. The
primary function of solvents in classical chemical syntheses is to facilitate mass transfer to
modulate chemical reactions in terms of reaction rate, yields, conversions and selectivity. An
ideal solvent facilitates the mass transfer but does not dissolve. For green chemistry, a
desirable solvent should be natural, nontoxic, cheap, non volatile and readily available. It
should have additional benefits of aiding the reaction, separation, recycling. Water, sub/super
critical water, ionic liquid, super critical fluid and expanded liquid are more preferable for
green chemistry [4]. Recently ‘‘switchable solvents (change their properties with different
needs)’’ is the new innovative discovery for green chemistry. Besides these solvents, other
synthetic, solvents such as fluorous and property-changing soluble polymer systems have
been evaluated as potential green alternatives [5, 6].
The synthetic methods that are still widely used are lacking in terms of materials,
energy efficiency and reaction parameters. The selective methodology has great impact on
the reaction condition and substrates in order to reduce reaction times and energy
requirements. However, the correlation between reactivity and toxicity has not been
incorporated into the design the synthetic methodologies [7]. In a chemical reaction, the
major concern is also the percentage of yield of desired product. Generally, the yield of desire
product should have very high as compared to the formation of bi-products or toxic product
(atom economy). Various attempts have been made to maximize the yield of the desired
products by developing reaction condition and catalyst.
New analytical tools are needed to analyse and environmental monitoring for better
knowledge. New methodologies and new measuring techniques for identification and
determination of trace and micro-trace components in samples with complex compositions
have enabled for discovery due to lack of proper analysis system. Therefore, it is necessary to
introduce the rules of green chemistry into chemical laboratories on a large scale. It is

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important to evaluate analytical methods not only in respect for the reagent, instrumental
costs and analytical parameters but also on the basis of their negative influence on the
environment. One of the good tools for such evaluation may be Life Cycle Assessment
(LCA).
Green chemistry is a philosophical approach that through application and extension
of the principles of green chemistry can contribute to sustainable development. The future
challenges are to find a sustainable resource, more efficient and environmental benign
scientific technologies for working with chemical processes. Green chemistry addresses such
challenges by inventing novel reactions that can maximize the desired products and minimize
by-products. Many new analytical methodologies are also described which are realized
according to green chemistry rules. They are useful in conducting chemical processes and in
evaluation of their effects on the environment. Lot of efforts has to be taken to design an ideal
process that starts from non-polluting starting materials which leads to no formation of
secondary products and do not required solvents to carry out the chemical conversion or to
isolate and purify the products. More environmentally friendly technologies at the research
stage have to be implemented on an industrial scale. Besides this, the success of green
chemistry depends on the training and education of a new generation of chemists.
REFERENCES
1. P. T. Anastas, C. J. Warner, “Green Chemistry: Theory and Practise” Oxford
University Press, Oxford, 1998.
2. Green Chemistry” United States environmental protection agency 28-02-2006. Retrieved
2011-03-23.
3. D. S. Argyropoulos, “Materials, Chemicals and Energy from Forest Biomass” ACS
Symposium Series 954, American Chemical Society, Washington, DC, 2007.
4. W. Wardencki, J. Cury, J. Namieœnik, Pol. J. Environ. Stud, 14(2005) 389-395.
5. J. A. Gladysz, D. P. Curran, I. T. Horvath, “Handbook of Fluorous Chemistry”, Wiley-
VCH, Weinheim, Germany, 2004.
6. R. H. Fish, “Fluorous biphasic catalysis: A new paradigm for the separation of
homogeneous catalysts from their reaction substrates and product” Chem. Eur. J. 5
(2004) 1677-1680.
7. N. J. Hoboken, P. Patnaik, “A Comprehensive Guide to the Hazardous Properties of
Chemical Substances”, 3rd ed. Ed, Wiley, 2007.

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IT15: Recent Advances in Hydrogen Fuel Technology
Mannam Krishna Murthy

Varsity Education Management Limited,
Ayyappa Society, Hyderabad-500 081.
E-mail : mannamkm@gmail.com
Problems with burning fossil fuels will discussed. Types of renewable energy sources
will be listed. Hydrogen, the ‘forever fuel’, that we can never run out of as it is so important
and the usage is eco-friendly. The methods of chemical production of hydrogen, hydrogen
storage and the innovative perspectives of hydrogen fuel technology will be summarized.
Recent advances in the potential use of hydrogen as green fuel for motor cars, trucks and
trains will be presented.
Key-words : Fossil fuels, Renewable energy sources, Hydrogen gas, Eco-friendly methods,
Recent advances.
IT16: STRATEGIES FOR PROTECTING CHEMICAL,

PHARMACEUTICAL & BIOLOGICAL INVENTIONS
Togapur Pavan Kumar

Senior Scientist
CSIR-Institute of Minerals and Materials Technology (IMMT)
Sachivalaya Marg, Bhubaneswar – 751013; Odisha, India
Email: pavantogapur@immt.res.in; Mobile: 8008105781
Inventions definitely arise when there is a necessity - in simple terms inventions are
nothing but the solutions to specific problems in the field of science and technology. There is
a progressive change in the factors contributing to global economies and as such we are in the
age of Innovations. In order to contribute towards this, one has to work out on the
possibilities of realizing the potential of research and scientific works and transform our skills
from knowledge contribution to the innovation contribution. In reference to this, as an
effective researcher one has to develop strategies for protection of inventions resulting from
pure, applied and interdisciplinary sciences and/or fields. This lecture will mainly focus on
the practical experiences leading to the way forward from basic research to innovative
outcomes.

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IT17: Catalysis Using Functionalized Nanomaterials for the Synthesis of

High Value Chemicals
Dr. Kalluri V.S. Ranganath

Dept. of Chemistry, Banaras Hindu University, Varanasi
E-mail: ranganath.chem@bhu.ac.in/ rangakvs@gmail.com
5-hydroxymethylfurfural (HMF) has been widely recognized as a key intermediate in

the production of biomass derived fuels and polymers. Its synthesis is based on the acid
catalyzed dehydration of sugars, mainly hexoses, which is highly non-selective when taking
place in aqueous media. Dehydration of monosaccharides and polysaccharides, which are
widely distributed in biomass, is the main synthetic pathway of HMF and also its derivatives.
Recently our group has reported the synthesis of 5-HMF from glucose and fructose using
various heterogeneous catalysts, including surface modified magnetite, MgO and graphene
oxide. Thus, I would like to present the synthesis of 5-HMF and its derivatives from the biomass
using various catalysts under heterogeneous conditions.
IT18: Strategic planning of proteins in ionic liquids: Future solvents for

enhanced stability of protein against multiple stresses
Pannuru Venkatesu
Department of Chemistry, University of Delhi, Delhi-110 007, India
e-mail: venkatesup@hotmail.com; pvenkatesu@chemistry.du.ac.in
Ionic liquids (ILs) have evolved as novel solvent systems fulfilling the requirements
of biotechnology and bioengineering. In recent years, large numbers of ILs have been
synthesized and their effect on protein stability has been illustrated. However, harsh process
conditions, such as temperature, pH, and presence of organic solvents, are the major barriers
to the effective use of enzymes in biocatalysis. We demonstrate the suitability of ILs as
potential media for enzymes, in which remarkable enhanced activity and improved stability
of proteins against multiple stresses were obtained. The catalytic activity of the enzyme in
presence of ILs was retained against several external stimulus, such as chemical denaturants
(H2O2 and GuHCl), and temperatures up to 120 °C. The observed enzyme activity is in
agreement with its structural stability, as confirmed by spectroscopic studies. Finally, it is
demonstrated that protein can be successfully recovered from the aqueous solution of ILs and
reused without compromising its yield, structural integrity and catalytic activity, thereby
overcoming the major limitations in the use of IL-protein systems in biocatalysis.
References:

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 A. Kumar, P. Venkatesu, Chemical Reviews, 2012, 112, 4283-4307.

 P. Venkatesu et. al, Green Chemistry, 2017, 19, 4900-4911.
 P. Venkatesu et. al, ACS Sustainable Chemistry & Engineering, 2020, 8, 604-612.
 J. Indrani, A. Rani and P. Venkatesu ACS Sustainable Chemistry & Engineering, 2017,
5, 8344-8355.
 A. Kumar, B. Kavya and P. Venkatesu, Phys. Chem. Chem. Phys. 2019, 21, 23269-
23282.
IT19: Electromembrane Extraction- A Novel Extraction Technique for

Pharmaceutical, Chemical, Clinical and Environmental Analysis
Vamsi Krishna Marothu*

Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu, Vijayawada.
Electromembrane extraction (EME) is a novel sample preparation technique in

pharmaceutical, chemical, clinical and environmental analysis. This technique uses
electromigration across artificial liquid membranes for selective extraction of analytes and
sample enrichment from complex matrices. This review focuses on the setup, general
procedure and parameters affecting the extraction efficiency of EME. An overview of
innovations in EME (on chip EME, low voltage EME, drop-to-drop EME, pulsed EME and
EME followed by low-density solvent based ultrasound assisted emulsification
microextraction) is also presented in this article and attention is focused on the use of EME
for pharmaceutical, chemical, clinical and environmental analysis.
Key words: EME, Innovations, Applications.

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 CHEMICAL
OP-1: VOLUMETRIC AND ACCOUSTIC STUDY OF 1-BUTYL-3-

METHYLIMIDAZOLIUM BIS (TRIFLUOROMETHYLSULFONYL)
IMIDE WITH 4-METHYL-2-PENTANONE
Dr. Raghavendra Muvva

Swire Coca-Cola USA, 12634 South 265 West Draper, UT 84020, USA
E-mail: Raghav.muvva@gmail.com;rmuvva@swirecc.com
Phone: (801) 816-5300 – O, (857) -(204) -(5706) (M)
Densities (ρ), speeds of sound (u) and refractive index (nD) data for the binary
mixtures containing the ionic liquid (IL) 1-Butyl-3-methylimidazolium bis(trifluoromethyl
sulfonyl)imide [Bmim][NTf2] with 4-methyl-2-pentanone over the entire range of
composition at T=(298.15 to323.15) K at atmospheric pressure are reported. From the
experimental data, the parameters like excess molar volume ( VmE ), excess isentropic
compressibilities (  Es ), deviation in speed of sound (uE), Rao’s molar sound functions (R),
deviation in refractive indices ΔnD, deviation in molar Refraction (Rm), and excess partial
molar volumes ( VmE,1 and VmE,2 ) and their limiting values at infinite dilution ( Vm0,1E and Vm0,2E ) are
calculated. Excess molar volume ( VmE ), and excess isentropic compressibilities ( K sE ) etc.,
were fitted to the Redlich - Kister polynomial equation. The experimental results are
discussed in terms of the formation of hydrogen bonds and existence of ion-dipole
interactions between components of the mixture. The formation of hydrogen bond in the
binary mixture system is confirmed by FT-IR spectroscopic studies.

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OP-2: A MEGLUMINE CATALYST BASED SYNTHESIS,

MOLECULAR DOCKING AND ANTI-OXIDANT STUDIES OF
DIHYDROPYRANO[3, 2-B] CHROMENEDIONE DERIVATIVES
N. Bakthavatchala Reddy1*, Grigory V Zyryanov1,2, A. Balakrishna3, C. Suresh Reddy4

1
Ural Federal University, Chemical Engineering Institute, Yekaterinburg, 620002, Russian
Federation.
2
I. Ya. Postovskiy Institute of Organic Synthesis, Ural Division of the Russian Academy of
Sciences, 22 S. Kovalevskoy Street, 620219 Yekaterinburg, Russian Federation.
3
Rajeev Gandhi Memorial College of Engineering and Technology (Autonomous ), Nandyal-
518501, Andhra Pradesh, India.
4
Department of Chemistry, Sri Venkateswara University, Tirupati-517 502, Andhra Pradesh,
India. Email:drbvreddyn@gmail.com
A simple method was employed for the synthesis of dihydropyrano [3,2-b]

chromenedione derivatives (4a-o) in high yields by condensation of 5,5-
dimethylcyclohexane-1,3-dione(1), different aromatic aldehydes (2a-o) and 5-hydroxy-2-
(hydroxymethyl)-4H-pyran-4-one (3), using Meglumine as a stable and reusable catalyst.
Meglumine, an amino sugar was employed as an environmentally benign catalyst, due to its
splendid properties such as being inexpensive, recyclable and biodegradable. The
accomplished protocol employs low catalyst loading, and easy work-up for the synthesis of 5-
hydroxy-2-(hydroxymethyl)-4H-pyran-4-onederivatives. A great asset is that without any
significant loss, the catalyst could be recovered and reused for extended synthetic steps. This
offer huge advantage to overcome recyclability issues. Our synthesized compounds were
analyzed by IR, 1H, 13C NMR, mass spectra and evaluated for their antioxidant properties by
1,1-Diphenyl-2-picryl hydrazyl radical (DPPH), Hydrogen peroxide(H2O2) and Nitric oxide
(NO) scavenging methods. The correlation in exhibition of antioxidant activity was effective
at all doses. The binding interactions and molecular docking studies for entitled compounds
were studied against 3MNG protein. 4k exhibited marked binding affinity with excellent
docking score of -7.6 K.cal/mol and emerged as a lead compound.
Scheme 1: Meglumine catalysed synthesis of Dihydropyrano [3,2-b]

chromenediones 4(a-o)

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OP-3: AN EFFECTIVE GREEN AND ECOFRIENDLY CATALYST FOR

SYNTHESIS OF BIS(INDOLYL)METHANES AS PROMISING
ANTIMICROBIAL AGENTS
Gundala Sravya1*, Grigory V. Zyryanov1,2, V. Padmavathi3, A. Padmaja3

1
Ural Federal University, Chemical Engineering Institute, Yekaterinburg, 620002, Russian Federation.
2
I. Ya. Postovskiy Institute of Organic Synthesis, Ural Division of the Russian Academy of
Sciences, 22 S. Kovalevskoy Street, 620219 Yekaterinburg, Russian Federation.
3
Department of Chemistry, Sri Venkateswara University, Tirupati-517 502, Andhra Pradesh,
India. Email:sravyasvu@gmail.com
An effective and suitable meglumine‐catalyzed high‐yielding process was considered

and engaged for the synthesis of new bis(indolyl)methanes at ambient temperature under
aqueous conditions. The catalytic reaction proceeds very smoothly. Clean reaction, ease of
product isolation/purification, easily available reactant and ecofriendly reaction conditions
are the notable advantages of the present methodology. All the title compounds were
characterized by IR, 1H, 13C NMR, and mass spectra. All the synthesized compounds were
tested for antimicrobial activity, and the results indicated that most of the synthesized
compounds exhibited excellent activity against the tested microorganisms. In this new series,
compound 3l, having nitro substituent on the aromatic ring, showed exceptional potent
inhibitory activity against Pseudomonas aeruginosa and Penicillium chrysogenum.
OP-4: Green One-pot Synthesis of Aminomethylene Bisphosphonates as

Antimicrobial and Antioxidant Agents
Tellamekala Sreekanth1, Cirandur Suresh Reddy1*

Department of Chemistry, Sri Venkateswara University, Tirupati - 517 502, India
*E-mail : csrsvu@gmail.com
A straightforward and greener approach has been developed for the synthesis of novel
aminomethylene bisphosphonates by one-pot reaction of diverse aryl/hereroaryl amines with
dialkyl phosphite and triethyl orthoformate using CuO nanoparticles as catalyst under
microwave irradiation and solvent free conditions [1-3]. This is an ecofriendly synthetic
approach for swift access to a library of diverse aminomethylene bisphosphonates. All the
synthesized new compounds were evaluated for their antimicrobial activity by disc diffusion
method and antioxidant activity by DPPH, H2O2 and NO methods. The title compounds
exhibited significant antioxidant and considerable antimicrobial activities.

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Scheme 1: Synthesis of aminomethylene bisphosphonates (4a-j)
References:
1. Mohan, G.; Santhisudha, S.; Reddy, N.M.; Sreekanth, T.; Murali, S.; Reddy, C.S.
Monatshefte für Chemie, 2017, 148, 1843-1851
2. Sudileti, M.; Nagaripati, S.; Gundluru, M.; Chintha, V.; Aita, S.; Wudayagiri, R.;
Chamarthi, N.; Cirandur, S. R. ChemistrySelect, 2019, 4, 13006-13011
3. Zhou, K.; Wang, R.; Xu, B.; Li, Y. Nanotechnology, 2006, 17, 3939- 3943
OP-5: DESIGN OF BIOSTABLE SCAFFOLD BASED ON COLLAGEN

CROSSLINKED BY DIALDEHYDE CHITOSAN WITH PRESENCE
OF GALLIC ACID
Pemo Bam, Anindita Bhatta and Ganesan Krishnamoorthy*

Natural Products Chemistry Groups, Chemical Sciences and Technology Division
CSIR-North East Institute of Science & Technology, Jorhat - 785 006, Assam, India
Tel: +91-376-2370017, Fax: +91-376-2370011
*Email: krishnamoorthyganesh@yahoo.com
In this study, we have prepared the biostable collagen scaffold which is crosslinked by
dialdehyde chitosan (DAC) with presence of Gallic acid (GA) and characterized its physico-
chemical, biostable and biocompatible properties. The digital photographic and scanning
electron microscopic (SEM) images of the prepared collagen scaffold is exposed well with
properly oriented interconnected porous natured structure. The appearance of diffraction
peaks showed slightly crystalline characteristic when compared to others. The differential
scanning calorimetric (DSC) and thermogravimetric analysis (TGA) measurements indicates
well significantly increased denaturation temperature (TD) and decreased decomposition rate.
FT-IR result suggests the structural integrity of collagen which favours the molecular
stability. The dialdehyde groups from DAC crosslinked with collagen functional groups that
increase the molecular crosslinking owing to the large number of amino groups in its
molecular chain. This scaffold exhibited 87% resistance against collagenolytic degradation
by collagenase. The results showed that the improved biostability which prevents the free
access of the collagenase to binds with the collagen triple helical chains. This scaffold

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confirm high biocompatibilities; enhanced cell proliferation and adhesions properties. This
results gains new insight into the collagen scaffold to improves the biostability. This could be
suitable method to preparation of collagenous biomaterials for tissue engineering
applications.
References
1. P. Bam, B. Anindita and G. Krishnamoorthy, Design of biostable scaffold based on
collagen crosslinked by dialdehyde chitosan with presence of gallic acid. International
Journal of Biological Macromolecules, 130, 2019, 836-844.
OP-6: INVESTIGATIONS ON CORROSION STUDIES OF AL6061

REINFORCED WITH TIO2 METAL MATRIX COMPOSITES IN
HYDROCHLORIC ACID MEDIUM
Latha.V1*, H.R.Radha1, P.V.Krupakara2 and R.La1kshmi1

1
Department of Chemistry, T.John Institute of Technology, Bangalore, Karnataka.
1
Department of Chemistry, Cambridge Institute of Technology, North Campus, Bangalore
Karnataka.*Email: latha@tjohngroup.com
This paper deals with the high corrosion resistance developed by the metal matrix
composites when compared with that of matrix alloy. Metal matrix composites made up of
aluminium and aluminium alloys are getting importance due to their applications in various
fields like automobile, aircraft and marine engineering. Matrix selected is Al6061 and
reinforcement selected is TiO2 particulates, which is a insulating material. The composites are
prepared using liquid melt metallurgy technique using vortex method. Preheated but uncoated
TiO2 particulates are added to the melt. The melt is stirred well degassed by adding
degasifying tablets. Metal matrix composites containing 2, 4 and 6 weight percentage of TiO2
are prepared. Bar castings are taken and are cut into cylindrical discs of 20mm diameter and
20mm thickness. Corrosion tests are conducted at room temperature (230 C) using
conventional weight loss method according to ASTM G69-80. The corrodents used for the
tests are of hydrochloric acid. Weight loss corrosion studies are carried out in different
concentrated solutions of 0.025, 0.05, 0.075 and 0.1N hydrochloric acid. Weighed specimens
dipped in corrodent solutions and are taken out at every 24 hours of interval up to 96 hours.
Corrosion rates are calculated using the formula 534DAT/W. In all concentrations of
hydrochloric acid solution, corrosion rate decreases with increase in exposure time for matrix
and metal matrix composites. As TiO2 content increases the composites become more
corrosion resistant due to insulating nature than matrix alloy. Therefore composite materials
are suitable for application in marine engineering than matrix alloy.
Key words: Composites, Vortex, Particulates, TiO2, Corrodent

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OP-7: MOLECULAR INTERACTIONS IN BINARY MIXTURES

CONTAINING HALO-SUBSTITUTED CARBONYL COMPOUND+
XYLENES AT VARIOUS TEMPERATURES
M.RadhaSirija1, Sk.Jareena2, D. Ramachandran*

1,*
Department of chemistry, Acharya Nagarjuna University, Guntur- 522 510, India.
Malineni perumallu educational society, Guntur, Andhra Pradesh 52201, India
Email: sireeja.maganti@gmail.com,dittakavirc@gmail.com
Thermo-physical, and excess parameters has a great impact to know the behaviour
and type of molecular interactions exist between the components of binary liquid mixtures at
different mole fractions and temperatures. Densities, viscosities and ultrasonic velocities of
binary liquid mixtures of halo-substituted carbonyl compounds with different Xylenes have
been measured over the entire composition range and at temperatures 303.15K, 308.15K,
313.15K and 318.15K. From experimental data, Excess parameters were calculated. The
obtained values were fitted to the Redlich-Kister polynomial equation. The parameters of
excess functions are found sensitive for the intermolecular interactions between the binary
mixtures. Theoretical values of viscosity of the binary mixtures were calculated using
empirical relations and theoretical equations.The relative merits of these relations and
theories were discussed.
Keywords: Ultrasonic velocity, viscosity, Redlich-Kister equation, carbonyl compounds
OP-8: GREEN AND EFFICIENT HETEROGENEOUS

NANOCATALYST FOR THE SYNTHESIS OF α -
AMINOPHOSPHONATES
S .H. Yasmin1 ,S.Murali1, G.Mohan1 & *C. Suresh Reddy1

Department of Chemistry, Sri Venkateswara University, Tirupati, 517502, India
Address correspondence: C. Suresh Reddy, Department of Chemistry, Sri Venkateswara
University, Tirupati 517 502, Andhra Pradesh, India. E-mail:csrsvu@gmail.com
An efficient novel synthetic protocol has been developed for the synthesis of diverse
biologically active α-aminophosphonates by reacting 2,3-dihydrobenzo[b] [1,4]dioxin-6-
amine with various substituted aldehydes and diethyl phosphite by Kabachinik – Field’s
reaction in the presence of an efficient heterogeneous nano MgO as catalyst under solvent
free conditions . Nano MgO has found to have many advantages like easy handling
,reusability and non-toxicity of the catalyst make the present protocol as an eco-friendly and

(RACPABS - 2020)
economically acceptable method for the synthesis of α-aminophosphonates. The products

obtained were good to very high yields. The products were characterized by IR, 1H, 13C, and
31
P NMR spectra and elemental analysis.
References
1. Sajadi SM, Kolo K, Hamad SM, et al. IET-Nanobiotechnol 2018; 13: 124.
2. Hirschmann, R.; Smith, A. B.; Taylor, C. M.; Benkovic, P. A.; Taylor, S. D.; Yager, K.
M.; Sprengler, P. A.; Venkovic, S. J. Science 1994, 265, 234-237.
3. Mabkhot, Y. N.; Aldawsari, F. D.; Al-Showiman, S. S.; Barakat, A.; Soliman, S. M.;
Choudhary, M. I.; Yousuf, S.; Mubarak, M. S.; Hadda, T. B. Chem. Central J. 2015, 9,
1-11. 37
OP-9: SMART DRUG DELIVERY MECHANISM USING

NANOROBOTS: THE EMERGING TOOLS IN MEDICINAL
Sikhinam Nagamani1 Dr.G.Muthaiah2 Dr.D.Ramachandran3

1
Assistant Professor, IT, Lakireddy Balireddy college of Engineering, Mylavaram.
2
Department of Chemistry, Acharya Nagarjuna University.
3
Associate Professor, Department of Chemistry, Acharya Nagarjuna University.
In todays world medical science is more and more improving with the
blessings of latest scientific discoveries. Nanotechnology is such a field which is
changing vision of life science . New automated procedures are being discovered with
new aspects of self-guided nanorobots. Nanorobot is an excellent tool for future
medicine. We can envision each day once you could inject billions of
those nanorobots that might float around in your body. Nanorobots could carry and
deliver drugs into defected cells. These nanorobots are repair tissues, clean blood vessels
and airways, transform our physiological capabilities, and even potentially counter act
the aging process. Many scientists performing on this bright field of nanorobots
especially on Alzheimer disease and cancer treatments. The engineering of molecular
products must be administered by robotic devices, which are termed Nano robots.

(RACPABS - 2020)
Nanorobos are also called nanobots. Nano robotics, sometimes mentioned as molecular
robotics, is an emerging research field as evidenced by recent topics within the literature.
A multifunctional platform supported nanorobots, with various sorts of nanomachines
will surely fight against major diseases like cancer, HIV etc. This abstract mainly focus
on nanorobots and its tools, mechanism, approaches and main futuristic applications
of an equivalent which mainly useful for medicinal and to develop new
formulations associated with nanotechnology to cure the major diseases.
OP-10: EFFECT OF TEMPERATURE ON PHYSICOCHEMICAL

PROPERTIES OF PROPYL ACETATE WITH 1-ALKYL-3-METHYL-
IMADAZOLIUM HEXAFLUROPHOSPATE
P Bharath a,*, P Suneethab, T.S.Krishnac, D.Ramachandrana

a
Department of Chemistry, Acharya Nagarjuna University,Nagarjuna Nagar-522510
b
Department of Chemistry, Government Degree College, Karvetinagaram-517582
c
Department of Physics, P.B.Siddhartha College of Arts & Science, Vijayawada – 520010
Andhra Pradesh, India *E-mail: bharath25211@gmail.com
The speeds of sound, u and densities, , of the binary mixture of 1-alkyl-3-methyl-

imadazolium hexaflurophospate (Where n=2,4,6) with propyl acetate including those of pure
liquids, have been determined from 0 to 0.1 mole fraction range of ILs with interval 0.1 at
298.15 - 323.15 K at pressure 0.1MPa. We used the measured data to assess the excess molar
E E
volumes, Vm , excess isentropic compressibility, s , excess speeds of sound, u E , excess
E
molar isentropic compressibility, Ks,m were calculated and fitted with Redlich-Kister
E E
equation. The excess partial molar volumes, Vm,1 and Vm,2 and excess partial molar
E E °E °E
isentropic compressibilities, Ks,m,1 and Ks,m,2 , excess partial molar volumes, Vm,1 and Vm,2
E °E
and excess partial molar isentropic compressibilities, Ks,m,1 and Ks,m,2 of the components at
infinite dilution have also been measured. The departure of measured properties has been
explored in terms of intermolecular interactions.
Key words: Density; speed of sound; Ethyl acetate; Ionic Liquids; intermolecular interaction

(RACPABS - 2020)
OP-11: ADSORPTION OF DISPERSE DYES ONTO ACTIVATED

CARBON PREPARED FROM AGRICULTURAL WASTE
T.V.Nagalakshmi1*, K.A.Emmanuel2, P. Paul Divakar3

1
Freshman Engineering Department, Lakireddy Bali Reddy College of Engineering,
Mylavaram -521230, A.P., India.
2
Department of Chemistry, Sir C.R.Reddy Autonomous College, Eluru-534 007, A.P., India
3
Department of Phisics, Sir C.R.Reddy Autonomous College, Eluru-534 007, A.P., India.
*Email ID: mannava_laxmi@yahoo.co.in
Disperse dyes are substantially water-insoluble non-ionic dyes for application to

hydrophobic fibers from aqueous dispersion. They are used predominately on polyester and
to a lesser extent on nylon, cellulose, cellulose acetate and acrylic fibers. Disperse dyes cause
risk of sensitization and elicitation to the body when colored clothes contact to the body. It
can also be regarded as potential allergens to children with suspected contact sensitization. In
the present study to prepare activated carbon from Jackfruit waste and named as JC600. Then
the JC600 was characterized by using different analytical techniques. The pore structures of
the resulting carbon were analyzed using N2 adsorption, X-ray diffraction (XRD) and
scanning electron microscope (SEM). Surface area was calculated by Brunauer–Emmett–
Teller (BET) equation. Thermal stability of carbon was analyzed by thermogravimetric
analysis (TGA) and temperature programmed desorption (TPD) studies. The nature of
functional groups present on surface of activated carbons was analyzed by FTIR and XPS
techniques. Finally the JC600 applied to decolorization of disperse dyes from textile effluents
by adsorption technique and observes the chemical kinetics and adsorption isotherms
involved in the adsorption of textile dyes.
Key words: Activated carbon, Disperse dye, adsorption, isotherms and kinetics
References:
1. Gopal, N., Asaithambi, M., Sivakumar, P. and Svakumar, V. 2014. Adsorption studies of
a direct dye using polyaniline coated activated carbon prepared from Prosopis juliflora.
J. Water process Eng. 2, 87-95.
2. Yadav, A.K., Kaushik, C.P., Haritash, A.K., Kansal, A. and Rani, N. 2006.
Defluoridation of groundwater using brick powder as an adsorbent. J. Hazard. Mater.
128, 289-293.
3. Holme, I. 1984. Developments in the chemistry and technology of organic dyes. In:
Griffiths, J. (Ed.), Ecological Aspects of Colour Chemistry. Society of Chemistry
Industry, Oxford, 111-128.

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OP-12: EVALUATION OF ULTRASONIC VELOCITY THEORIE ON

OF BINARY MIXTURES CONTAINING PROPIOPHENONE (PPH)
WITH ISOMERIC XYLENES AT TEMPERTURES 303.15K TO 318.15K
Nanduri Gayatri Devi 1 , N.V.N.B.Srinivasa Rao2,D.Ramachandran3

1
Ch.S.D.St.Theresa’s(A) College for Women, Eluru.
2
DRG Government Degree College, TadepalliGudem
3
Acharya Nagarjuna University, Guntur,
balajivishy@yahoo.com;Ph.No.+919492702645
Densities ’ρ’, Ultrasonic speeds of sound ‘u’ of binary mixtures containing

Propiophenone (PPH) with o- xylene, m-xylene and p-xylene were measured over the entire
composition range at temperatures from 303.15K to 318.15K and at atmospheric pressure
0.1MPa. Experimental data of ultrasonic velocity used to compute the theoretical velocities
by using various theories like Nomoto’s relation (UNOM), Impedance relation (UIMP), Van
Dael and Vangeel’s relation (UVDV), Rao’s specific velocity relation (URAO) and Junjie’s
theory (UJUN). The results are in good agreement with the experimental data. The relative
percentage error, Chi-square test for goodness of fit and the molecular interaction parameter
(α) values for non-ideality in the binary mixtures were computed and discussed in terms of
intermolecular interactions between the molecules of the binary mixtures. Ultrasonic velocity
of the binary liquid mixtures is the vital tool to measure the mechanical stability of liquids
and to understand the molecular interactions, physico-chemical bevaiour in pure, binary and
higher order multi component liquid mixtures [1]. The ultrasonic velocity u (m/sec) in a
liquid or a liquid mixture is mainly determined by its intermolecular properties [2]. It is clear
that the measurement of speed of sound can supplement and replace measurements on other
physical properties in studies of molecular structure [3]. Theoretical evaluation of ultrasonic
velocity values of binary mixtures with the experimental data indicates the behavior of
molecular interactions and it is used to develop theoretical models for liquids.
Keywords: Ultrasonic speed of sound; molecular interaction parameter; Velocity theories;
Chi-square test; relative percentage error.
References:
[1 ] N. Santhi, P.L.Sabarathinam, J.Madhumitha, G.Alamelumangai, M.Emayavaramban,
Theoretical Evaluation of Ultrasonic Velocity in Binary Liquid Mixtures of Alcohols [S]
+ Benzene, Int. Lett. Chem. Phiysics Astron. 2 (2013) 18–35. doi=10.1.1.1021.9571
[2] J. BERTIL, Ultrasonic Velocity in Liquids and Liquid Mixtures, J. Chem. Phys. 20
(1952) 927–928. doi:10.1063/1.1700614.
[3] R.T. Lagemann, W.S. Dunbar, Relationships between the velocity of sound and other
physical properties of liquids, J. Phys. Chem. 49 (1945) 428–436.
doi:10.1021/j150443a003.

(RACPABS - 2020)
OP-13:
13: SILVER DECORATED REDUCED GRAPHENE OXIDE NANO
COMPOSITE WITH A VALUABLE BIOMEDICINAL PLANT,
KIGELIA AFICANA (KA) FOR SYNTHESIS AND ITS
CHARACTERIZATION
Murali Krishna and Dr. B. Hari Babu*

K. Chandra Mohan, K. Bala Murali
Dept. of Chemistry, Acharya Nagarjuna University, Guntur, AP, India
India-522510.
A green synthesis of silver decorated reduced raphene oxide nanocomposite using a

valuable biomedicinal plant Kigelia Africana (KA) stem extract was developed. The present
green synthesis is a very cost effective, safe, nontoxic, eco-friendly
eco friendly route which can be used
for the large scale synthesis. The method involves dispersion of aqueous graphene oxide oxide-
silver ions in aqueous stem extract for reduction
reduction leading to reduced graphene oxide
oxide-silver
(KRG-Ag)Ag) nanocomposite. The synthesized KRG-Ag
KRG Ag nanocomposite characterized by using
UV-Vis
Vis spectrophotometer, Fourier Transform Infra-red
Infra red Spectroscopy (FT
(FT-IR), X-ray
diffraction studies (XRD), Raman spectroscopic
spectroscopi studies and the HR-SEM SEM and HRHR-TEM
analysis.
Keywords: Silver nanocomposites, Kigelia Africana stem extract, green synthesis.
Ref:
1. A facile green reduction of graphene oxide using Annona squamosa leaf extract, Carbon
Letters, 21, 74-80,2017.
2. Synthesis of multifunctional graphene exhibiting excellent sonochemical dye removal
activity, green and regioselective reduction of cinnamaldehyde, Materials Letters, 293,
127224-127227, 2019.
3. A Facile Green Reduction for Graphene‐Silver
Graphene Silver Nanocomposite Using Betel Leaf
Extract for the Photocatalytic degradation of Water Pollutants,
Pollutants Chemistry Select
Select, 34(2),
11172-11176, 2017.
4. Phytochemical screening, anti-diabetic
anti and anti-oxidant
oxidant activities of Kigelia Africana
(LAM) and Sterculia L, Rasayana Journal of chemistry, 12(2), 907-914,2019.
907 914,2019.
Department of Chemistry, Acharyaa Nagarjuna University, Nagarjuna Nagar-522510,

522510, Guntur
Guntur, A.P. 30
(RACPABS - 2020)
OP-14: METHOD DEVELOPMENT AND VALIDATION OF

VENETOCLAX IN HUMAN PLASMA BY LC-ESI-MS/MS
P. Haritha*
*
Department of Chemistry, Gudlavalleru Engineering College, Gudlavalleru-521356,
India.haritha.potluri@gmail.com
A sensitive method of liquid chromatography–tandem mass spectrometry was

established for the experimental determination of venetoclax in human plasma utilising
venetoclax-D8 as an internal standard. The Xbridge column C18, 50 × 4.6 mm, 5 m was
used for chromatographic separation by using 10mM Ammonium formate and methanol as
isocratic mobile phase 20:80 (v/v) ratio. The flow-rate selected was 0.7 mL/min. Venetoclax
and venetoclax-D8 are identified in multiple reaction monitoring (MRM) positive mode with
proton adducts at m/z 869.53 553.21 and m/z 877.14  553.23 respectively. Liquid-Liquid
extraction technique was used for the effective extraction of drug as well as internal standard.
The developed technique was established in a linear concentration range of 5.0-5000.0
pg/mL. Intra and inter-day precision are found to be 0.7 to 1.90 % and 0.7 to 2.0 % for
venetoclax and venetoclax-D8 respectively. Accuracy was found to be within 98.6 to 101.99
% and 99.17 to 101.14 % for venetoclax and venetoclax-D8 respectively. It was observed that
throughout bench top studies, postoperative stability studies and freeze-thawing cycles,
venetoclax retained stability.
Key words: Venetoclax, Bioanalytical, Mass spectrometry, Pharmacokinetic study
References
1) Anderson M.A., Huang D. and Roberts A.: Targeting BCL2 for the treatment of
lymphoid malignancies. Semin Hematol. 2014, 51, 219–227.
2) Cang S., Iragavarapu C., Savooji J., Song Y. and Liu D.: ABT-199 (venetoclax) and
BCL-2 inhibitors in clinical development. J. Hematol. Oncol. 2015, 8, 129.
3) Roberts A.W., Davids M.S., Pagel J.M., Kahl B.S., Puvvada S.D., Gerecitano J.F.,
Kipps T.J., Anderson M.A., Brown J.R. and Gressick L. et al.: Targeting BCL2 with
Venetoclax in Relapsed Chronic Lymphocytic Leukemia. N. Engl. J. Med. 2016, 374,
311–322.
4) Souers A.J., Leverson J.D., Boghaert E.R., Ackler S.L., Catron N.D., Chen J, Dayton
B.D., Ding H., Enschede S.H. and Fairbrother W.J. et al.: ABT-199, a potent and
selective BCL-2 inhibitor, achieves antitumor activity while sparing platelets. Nat. Med.
2013, 19, 202–208.

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OP-15: EVALUATION OF ULTRASONIC VELOCITY THEORIES ON

BINARY LIQUID MIXTURES OF MORPHOLINE WITH AMIDES AT
TEMPERATURES T = (303.15 TO 318.15) K
D.Bala1 , V. Anand Kumar, Dr.D.Ramachandran2 .

Bala.anu163@gmail.com,dittakavirc@gmail.com,Ph.No.7036440831
Densities ’ρ’, Ultrasonic speeds of sound ‘u’ of binary mixtures containing

Morpholine with formamide, N-methyl acetamide, N,N-dimethyl acetamide were measured
over the entire composition range at temperatures from 303.15K to 318.15K and at
atmospheric pressure 0.1MPa. Experimental data of ultrasonic velocity used to compute the
theoretical velocities by using various theories like Nomoto’s relation (UNOM), Impedance
relation (UIMP), Van Dael and Vangeel’s relation (UVDV), Rao’s specific velocity relation
(URAO) and Junjie’s theory (UJUN). The results are in good agreement with the experimental
data. The relative percentage error, Chi-square test for goodness of fit and the molecular
interaction parameter (α) values for non-ideality in the binary mixtures were computed and
discussed in terms of intermolecular interactions between the molecules of the binary
mixtures. Ultrasonic velocity of the binary liquid mixtures is the vital tool to measure the
mechanical stability of liquids and to understand the molecular interactions, physico-
chemical bevaiour in pure, binary and higher order multi component liquid mixtures [1]. The
ultrasonic velocity u (m/sec) in a liquid or a liquid mixture is mainly determined by its
intermolecular properties [2]. It is clear that the measurement of speed of sound can
supplement and replace measurements on other physical properties in studies of molecular
structure [3]. Theoretical evaluation of ultrasonic velocity values of binary mixtures with the
experimental data indicates the behavior of molecular interactions and it is used to develop
theoretical models for liquids.
[1 ] N. Santhi, P.L.Sabarathinam, J.Madhumitha, G.Alamelumangai, M.Emayavaramban,
Theoretical Evaluation of Ultrasonic Velocity in Binary Liquid Mixtures of Alcohols
[S] + Benzene, Int. Lett. Chem. Phiysics Astron. 2 (2013) 18–35. doi=10.1.1.1021.9571
[2] J. BERTIL, Ultrasonic Velocity in Liquids and Liquid Mixtures, J. Chem. Phys. 20
(1952) 927–928. doi:10.1063/1.1700614.
[3] R.T. Lagemann, W.S. Dunbar, Relationships between the velocity of sound and other
physical properties of liquids, J. Phys. Chem. 49 (1945) 428–436.
doi:10.1021/j150443a003.

(RACPABS - 2020)
OP-16: PT-SN NANOPARTICLES DECORATED BINARY

POLY(AMINE TERMINATED CYCLOPHOSPHAZENE-CO-
BENZIDINE DISULFONIC ACID)-CARBON NANOTUBE COMPOSITE
FOR METHANOL ELECTROOXIDATION IN ALKALINE MEDIUM
D.Prasannaa*, S. Ratna Kumaria, Ch. Rajinia, K.Krishnadevi, V.Anuradhaa,c*

a
Department of Chemistry, Vignan’s Nirula Institute of Technology and Sciences for
Women, Guntur, India
b
Division of Chemistry, Department of Scienceand Humanities, Vignan’s Foundation for
Science,Technology and Research (VFSTR), Vadlamudi, Guntur, India
c
Department of Chemistry, Vignan’s Degree and P.G. College, Guntur, India
Corresponding Authors: Vejendla Anuradha, chema2013@gmail.com and D.Prasanna,
d.prasanna0103@gmail.com
Keywords:Platinum-tin, methanol, electrooxidation The present study is focused on

the preparation of binary poly(amine terminated cyclophosphazene-co-benzidine disulfonic
acid)-carbon nanotube (Poly(ATCP-co-BZDA)-CNT) compositeby usingthe monomers
namely amine terminated cyclophosphazene (ATCP), and 2,2′-benzidinedisulfonic acid
(BZDA) as a novel catalyst support for methanolelectrooxidation. In this view, Platinum (Pt)
and platinum-tin (Pt-Sn) nanoparticles were deposited overPoly(ATCP-co-BZDA)-
CNTcomposite material through a formaldehyde reduction method using hexachloroplatinic
acid hexahydrate and stannous(II) chloride as metal precursor materials. The platinum
nanoparticles and platinum-tin depositedPoly(ATCP-co-BZDA)-CNTcomposites were
characterized by various analytic techniques. The activity and stability of the Pt/Poly(ATCP-
co-BZDA)-CNTand Pt-Sn/Poly(ATCP-co-BZDA)-CNTcatalysts were analyzed by cyclic
voltammetry and chronoamperometry techniques in the presence of 0.5 M methanol and 0.5
M KOH solution. From the results, it has been concluded that the Pt/ Poly(ATCP-co-BZDA)-
CNT and Pt-Sn/Poly(ATCP-co-BZDA)-CNTelectrocatalysts exhibit significant higher anodic
oxidation current and lower onset potential when compared to those of Pt loaded Vulcan
carbon and CNT materials

(RACPABS - 2020)
OP-17: ANTI-HYPERGLYCEMIC EFFECT OF CHRYSIN ON

GLUCOSE LOADED RATS
S.Kalyani1 , Dr.D.Ramachandran2
1.Department of Bio-Chemistry, 2.Department of Chemistry
Acharya Nagarjuna University, Guntur-522001
Diabetes Mellitus, characterized by persistent hyperglycemia, is a heterogeneous

group of disorders of multiple etiologies. It affects the human body at multiple organ levels
thus making it difficult to follow a particular line of the treatment protocol and requires a
multimodal approach. The increasing medical burden on patients with diabetes-related
complications results in an enormous economic burden, which could severely impair global
economic growth in the near future. This shows that today’s healthcare system has
conventionally been poorly equipped towards confronting the mounting impact of diabetes on
a global scale and demands an urgent need for newer and better options. Chrysin belongs to a
class of chemicals called flavonoids. It occurs naturally in plants such as the passionflower,
silver linden, and some geranium species; and in honey and bee propels (glue). The present aim
is to evaluate the anti-hyperglycemic effect of chrysin on glucose loaded rats.
Key words: Glucose, Diabetes, Chrysin and Glucose Loaded Rats
1. Ramírez-Espinosa JJ, Saldaña-Ríos J, García-Jiménez S, Villalobos-Molina R, Ávila-
Villarreal G, Rodríguez-Ocampo AN, Bernal-Fernández G, Estrada-Soto S. Chrysin
induces antidiabetic, antidyslipidemic and anti-inflammatory effects in athymic nude
diabetic mice. Molecules. 2018 Jan;23(1):67.
2. Vinayagam R, Xu B. Antidiabetic properties of dietary flavonoids: a cellular mechanism
review. Nutrition & metabolism. 2015 Dec; 12(1):60.

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OP-18:UNDERSTANDING OF MOLECULAR INTERACTIONS

BETWEEN ETHYL ACETATE AND 1-BUTYL-3-METHYL-
IMIDAZOLIUM BIS(TRIFLUORO METHYL SULFONYL)IMIDE: A
THERMOPHYSICAL STUDY
P.V.S.S Rama Raoa, T.S.Krishnab,*, D. Ramachandranc,¥

a
Research Scholar, Rayalaseema University, Kurnool-518002, Andhra Pradesh, India
b
Department of Physics, P.B.Siddhartha College of Arts & Science, Vijayawada-520 010,
c
Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510 Guntur,
Andhra Pradesh, India;*E-mail: sritadikonda@gmail.com, dittakavirc@gmail.com
In this work, densities, speeds of sound and refractive index of pure and their binary
blends of 1- butyl -3-methylimidazolium bis(trifluromethylsulfonyl)imide ionic liquid (IL)
with Ethyl acetate (EtOAc) have been measured at various temperatures at atmospheric
pressure 0.1 MPa. With help of experimental results, discussed the molecular interactions and
effect of temperature on interactions through excess properties. The excess properties
adequately fitted with Redlich-Kister polynomial equation. To study the non-ideal behaviour
applied Prigogine−Flory−Patterson (PFP) theory. For more understanding and confirmation
of molecular interactions authors used FTIR study at room temperature.
OP-19: NEW RP-HPLC METHOD DEVELOPMENT VALIDATION

AND SUBSEQUENT FORCED DEGRADATION STUDIES FOR THE
SIMULTANEOUS ESTIMATION OF CHLORPROPAMIDE AND
PHENFORMIN IN PHARMACEUTICAL FORMULATIONS
B.Sowjanya* & Dr. K. Rambabu┼

*Department of Chemistry, SRK Insitute of Technology, Enikepadu, Andhra Pradesh-521108,
┼Department of Chemistry, R V R & J C College of Engineering, Chowdavaram, Guntur,
Andhra Pradesh – 522019.
This abstracts entails a systematic, sensitive and cost-effective stability-indicating

method for the simultaneous estimation of chlorpropamide and phenformin in
pharmaceutical formulations. Separation of chlorpropamide and phenformin was
successfully achieved on a Kromasil C8 (250 mm x 4.6mm x 5µm) in an isocratic mode
utilizing Methanol: Acetonitrile: 0.01M potassium dihydrogen phosphate (35:30:35% v/v) at
a flow rate of 1.0mL/min and UV detection at 218nm. The developed method was validated
and found to be precise, robust, accurate, linear (range of 5.0-30μg/ml for chlorpropamide
and phenformin respectively), and specific for degraded products ensuring suitability of the

(RACPABS - 2020)
method for quantitative determination of chlorpropamide and phenformin. The

experimentally observed values of LOD and LOQ of chlorpropamide and phenformin were
also quite lower than those reported in the literature. Forced degradation studies were
performed for the developed method as per ICH guidelines. Statistical comparison was done
between the proposed method and the reported one for chlorpropamide and phenformin
where no significant difference was found between the two methods. This RP-HPLC method
demonstrated high degree of practical utility for the simultaneous estimation of quantitative
analysis of chlorpropamide and phenformin in commercial tablets.
OP-20: DESIGN, SYNTHESIS AND BIOLOGICAL EVALUATION OF

AMIDE FUNCTIONALITY BEARING 1, 2, 4- THAIDAZOLE- 1, 2, 4-
TRIAZOLE DERIVATIVES AS ANTI- CANCER AGENTS
Yazala Jyothsna Pragathi,1 Reddymasu Sreenivasulu,2 Deekala Veronica,1 Rudraraju

Ramesh Raju*1
1
Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510,
Andhra Pradesh, India.
2
Department of Chemistry, University College of Engineering (Autonomous), Jawaharlal
Nehru Technological University, Kakinada – 533 003, Andhra Pradesh, India.
* Email: rrraju1@gmail.com
A novel library of amide functionality having 1, 2, 4- thiadiazole- 1, 2, 4- triazole

analogues were designed, synthesized, and structures were characterized by 1H NMR, 13C
NMR, and Mass (ESI- MS) spectral data. Further, all compounds were evaluated for their
anticancer activities against four different cancer cell lines including breast cancer (MCF- 7,
MDA MB- 231), lung cancer (A549) and prostate cancer (DU- 145) by MTT reduction assay
method and Etoposide act as a standard drug. The results confirmed that majority of the
synthesized compounds showed moderate to potent anticancer activities aligned with four
cell lines. Among the synthesized Triazole compounds and displayed more potent activity
along with inhibitory concentration values ranging from 0.10±0.084 µM to 11.5±6.49 µM
than the standard IC50 values, which ranges from 1.91 ± 0.84 µM to 3.08 ± 0.135 µM
respectively.
Keywords: Letrozole, 3,5-bis(pyridin-3-yl)-1,2,4-thiadiazole, 1,2,4-triazole, 1,2,4-thiadiazole
and anticancer activity.

(RACPABS - 2020)
OP-21: MOLECULAR INTERACTIONS IN BINARY MIXTURES

CONTAINING DIALKYL CARBONATE ESTERS WITH CHLORO
TOULENES AT VARIOUS TEMPERATURES
A. Venkateswara Rao1, M. Radha Sirija2, N. Venkateswara Rao, D. Ramachandran+

+, 1,2
Email: vk.adapala1@gmail.com, dittakavirc@gmail.com
Densities, viscosities and ultrasonic velocities of binary mixtures containing dialkyl

carbonate esters with chloro toulenes were measuredat various temperatures and ambient
pressure. From experimental data, parameters were calculated. The obtained values were
fitted to the Redlich-Kister equation. The parameters of excess functions are found
sensitivefor the intermolecular interactions between the binary mixtures. Theoretical values
of viscosity of the binary mixtures were calculated using empirical relations and theoretical
equations. The relative merits of these relations and theories were discussed.
OP-22: STRUCTURAL AND SPECTRAL INVESTIGATIONS OF VO2+

ION DOPED CD3(BO3)2 NANOCOMPOSITES FOR
OPTOELECTRONIC APPLICATIONS
P.N.V.V.L.Pramila Rani 1, D.Ramachandran1, R.V.S.S.N.Ravikumar2

1
Department of Chemistry, AcharyaNagarjuna University, Nagarjuna Nagar, India- 522510
2
Department of Physics, AcharyaNagarjuna University, Nagarjuna Nagar, India- 522510
Email: prameelarani585@gmail.com
Cadmiumborate (Cd3(BO3)2) nanocomposites and VO2+ doped(Cd3(BO3)2)

nanocompositeswere synthesized by chemical precipitation method. The X-ray diffraction
patterns of the prepared nanocomposites exhibited partial crystallinity with amorphous
background and the patterns displayed orthorhombic crystal structure. Diffraction peaks of
orthorhombic crystal structure of cadmium borate are noticed in VO2+ doped
Cd3(BO3)2nanocomposites, corresponding lattice cell parameters are evaluated. The
substitution of VO2+ enhanced the lattice constant and volume of the unit cell and is
attributed to the differences in the ionic radii of the elements. The average crystallite sizes are
obtained using Debye Scherer’s expression and are in the range of 30 - 60 nm. The surface
morphology of the pure and VO2+ doped nanocomposites revealed stone like shaped particles
with an average particle size of 43nm. The substitution of VO2+ inhibited the grain growth of
the nanocomposites and the elemental composition of these composites is confirmed from

(RACPABS - 2020)
energy dispersive spectra. The optical absorption spectra of VO2+ doped Cd3(BO3)2
nanocomposites indicated the metal ion is surrounded by a distorted octahedron of oxygen
ligands. The crystal field Dq and inter electronic repulsion parameters B and C are evaluated.
The evaluated Dq value also suggests a Octahedral coordination with tetragonal compression
of VO2+ ions with the ligand (host). Photo luminescence (PL) spectra of these
nanocomposites exhibited clearly that doping of VO2+ in the host matrix lead to significant
changes in PL intensity. Electron paramagnetic resonance (EPR) revealed that ∆║/∆┴ ratio is
greater than unity and it confirms that the VO2+ions are tetragonally distorted octahedral,
which can be attributed to Vinadyl into host lattice at octahedral environment of oxygen with
tetragonal distortion (C4v). FT-IR spectra for both pure and doped Cd3(BO3)2 demonstrated
the characteristic vibrations of cadmium borate. The obtained response from structural,
optical and EPR studies of the prepared nanocomposites are promising for the applications in
optoelectronic devices.
OP-23: DESIGN, SYNTHESIS OF SULPHONAMIDE FUSED 2-

(ISOXAZOL-3-YL)-1H-IMIDAZOLES AND THEIR IMPACT ON
ANTICANCER ACTIVITIES
B. Ravinaika, P.T.S.R.K.PrasadaRaob, D.Ramachandran*c and M.V. BasaveswaraRaod

a
Department of Chemistry, Krishna University, Machilipatnam – 521 001, Andhra Pradesh,
India.
.b
Department of Chemistry, PB Siddartha College of Arts & Science, Vijayawada – 520010,
A.P, India.
c
Department of Chemistry, AcharyaNagarjuna University, Nagarjuna Nagar – 522 510, A.P,
India. dittakavirc@gmail.com
d
Department of Chemistry, Dr. MRAR. PG Centre, Krishna University, Nuzvid – 521 201,
A.P, India.
Cancer is a most significant malignant disease and second leading cause of death in
developed countries after heart disease. It is a group of diseases involving abnormal cell
growth with the potential to invade or spread to other parts of the body. Now a day’s three
types of cancer treatments are available such as chemotherapy, surgical removal and radiation
therapy. Among these, chemotherapy is the most important and efficient for the treatment of
cancer.
Methods: Anticancer activities of all synthesized derivatives were determined by MTT assay
method.
Results: Among them, 10b, 10c, 10d, 10e, 10g, 10h and 10i compounds showed more potent
anticancer activity against human cancer cell lines.

(RACPABS - 2020)
2-iodoxy-
N Br THF, 0 oC, 1h N OH benzoic acid(IBX)
DMSO, rt, 5h N O
CHO Mg
N 5 rt, 8h N 7
H H N
6 H
4
NH2OH.HCl
tBuOH, TBAI,H2O
p-TsOH, reflux, 5h
O Cl
S
N N O O
MeCN, Cs2CO3 N N O
10a; R = H R
10b; R = 2,4,5-trimethoxy N rt, 6h N
10c; R = 3,5-dichloro O S 9a-j H 8
O
10d; R = 4-methoxy
10e; R = 4-chloro 10a-j 9a; R = H
10f; R = 4-bromo R 9b; R = 2,4,5-trimethoxy
10g; R = 4-cyano 9c; R = 3,5-dichloro
10h; R = 4-nitro 9d; R = 4-methoxy
10i; R = 3-nitro 9e; R = 4-chloro
10j; R = 4-methyl 9f; R = 4-bromo
9g; R = 4-cyano
9h; R = 4-nitro
9i; R = 3-nitro
9j; R = 4-methyl
Scheme 1: Synthesis of Sulphonamide fused 2-(Isoxazol-3-yl)-1H-imidazole derivatives

[1] Garcia,M.,Jemal,A.,Ward,E.M., Globa cancer facts & figures.American Cancer
Society,Atlanta, 2007.
[2] Hanahan, D.; Weinberg, R.A. The hall marks of cancer. Cell,2000, 100, 57-70.
[3] Ravinaik.B.,D.Ramachandran and M.V.BasaveswaraoRao.,Russian Journal of General
Chemistry,2019,89,3, pp.511-516
[4] Ravinaik.B.,D.Ramachandran and M.V.Basaveswarao Rao.,Russian Journal of General
Chemistry,2019,89,5, pp.1003-1008
OP-24: QUALITATIVE AND QUANTITATIVE ANALYSIS OF WATER

SAMPLES OF KIDNEY EFFECTED AREA USING ICPMS
TECHNIQUE
T.P.Raju1*, N.Giridhar Babu1, Ch.Ch.Srinivas1,R.R.Vedantam,2 N.Lakshmana Das3

1
Department of physics, Acharya Nagarjuna University, Nagarjuna Nagar Guntur-522510, AP.
2
Department of Basic sciences, Gudlavalleru Engineering college, Gudlavalleru-521356, AP.
3
Gitam Institute of Science, Gitam University,Visakhapatnam-530045, AP, India.
Corresponding author : tupatiraju369@gmail.com; 9550284805
Water samples are collected from different villages affected with Chronic Kidney
Disease (CKD) in Uddanam area of Srikakulam District, Andhra Pradesh, India. These
samples are analyzed using Inductively Coupled Plasma Mass Spectroscopy (ICPMS) for the
presence of trace elements. The trace elements Li, Be, Al, V, Cr, Mn, Fe, Ni, Cu, Zn, Ga, As,
Se, Rb, Sr, Cd, Hg, Pb and U are identified. The concentrations of the elements that cause

(RACPABS - 2020)
CKD are compared with the standard concentrations set by WHO. The impact of each
element on CKD is analyzed.
Water samples are analyzed by ICPMS at center for studies on Bay of Bengal, Andhra
University,and Visakhapatnam.Certiprep, multi-elemental
multi elemental standard is used to caliber the
instrument by serial dilution. Rathodium is used as internal standard to check the
performance of instrument. ICPMS is an analytical technique for elemental determination.
ICPMS combines a high temperature inductively coupled plasma with mass spectrometer.
The high temperature converts the atoms of the material into ions. These ions are separated
and detected by the mass spectrometer. This method has high speed,precision and sensitive.
Figure 1: Graphical representation of Pb

1. Reddy DV A. Chronic kidney disease in two coastal districts of Andhra Pradesh, India:
Role of drinking water. Environmental Geochemistry and Health. 2013(35):439
2013(35):439-454.
2. WHO .Guidelines for drinking water quality,World Health Organisation, Zwiterland,
2006: pp 335-397.
OP-25:
25: NEW TECHNIQUES FOR THE IDENTIFICATION OF
POLLUTANTS OF CERTAIN ELEMENTS: A STUDY
M.V.K.Mehar1, P.Suresh2
1
Dept.of Physics,P.R.Govt.College(A),Kakinada,E.G.Dt.,AP
2
Dept.of Chemistry,JNTUK,Kakinada,E.G.Dt.,AP
The Present study analyse the various elements contamination in various forms which
are analyzing by various methods. In such a way Identification of Arsenic in various forms
such as H3AsO3, H2AsO3, HAsO3, H3AsO4, H2AsO4 and HAsO4. The arsenic concentration
in groundwater varies with geographical locations. Ore of arsenic like H3AsO3, is more
prominent in various places. The chemical pollutants may include both organic and Inorganic
materials. Different Procedures are there for handling heavy metals water pollution which
encompass electro-osmosis,
osmosis, ionexchange, electro-kenetic,
electro kenetic, sludge activation, as well as
phytoextraction. The costly other techniques, such as phytoremediation.

522510, Guntur
Guntur, A.P. 40
(RACPABS - 2020)
OP-26: RAPID QUANTIFICATION OF THE APIXABAN IN DOSAGE

FORMS BY STABILITY INDICATING REVERSE PHASE-HIGH
PERFORMANCE LIQUID CHROMATOGRAPHY
T.Suresh* & Dr. K. Rambabu┼

*┼Department of Chemistry, R V R & J C College of Engineering, Chowdavaram, Guntur,
Andhra Pradesh – 522019.
A new stability indicating reverse phase high performance liquid chromatographic

method for the estimation of apixaban in dosage form has been developed. Chromatography
was carried out on a Thermo BDS, C8, 150 X 4.6, 5µ with mobile phase mobile phase
consisting of mixture of potassium dihydrogen phosphate (pH 4.2) and Acetonitrile
(65:35%v/v) at a flow rate was 1.0 mL.min-1 with UV detection wavelength of 259nm at
ambient temperature. The retention time for Apixaban was found to be 3.133min
respectively. The proposed RP-HPLC method was found to be linear in the concentration
range of concentration range of 8.0 -48µg/ml for apixaban respectively. The method was
validated as per ICH guidelines and was found to be suitable for bioequivalence and
pharmacokinetic studies.
OP-27: KOLLERU LAKE: ITS PRESENT WATER QUALITY STATUS
Dr.K.Soujanya¹, Dr.D.Ramachandran²
¹Department of Chemistry, CMR College of Engineering& Technology, Hyderabad,
Telangana
Email: Soujanya.kaki@cmrcet.org;; dittakavirc@gmail.com
Kolleru, which is one of the largest and shallow fresh water lakes in India located on
the deltas of Godavari and Krishna rivers in the state of Andhra Pradesh (16º 15´ and 16º 30´
N and 81º 00´ and 81º 25´ E). It is a wetland of international importance recognized under
Ramsar Covention, Iran (1971) that covers an area of 308 km2 [1]. It receives about 6.5 to
1.1 M cusecs of water from catchments. It has number of streams that open into it like,
Tammileru, Budameru, Ramileru, etc., but only outlet is Upputeru which connects the lake to
Bay of Bengal. Assessment of Monthly and seasonal changes in surface water quality is a
vital aspect for evaluating earthly variation of water Quality level due to natural inputs of
point and non-point sources.. In this study, the physico-chemical parameter of water such as
Temperature, pH, Dissolved Oxygen (DO), Biochemical Oxygen Demand (BOD), Chemical
Oxygen Demand (COD), Total Dissolved Solids (TSS), Hardness, Alkalinity, Conductivity,

(RACPABS - 2020)
Calcium, Magnesium, Chloride and Turbidity are considered for analysis during the period of
June 2018 to July 2019 both season and month wise [2]. Water samples were collected from
ten sampling stations of lake and analysed by using AAS & ICP-OES Spectrometers. For
calculating the Water Quality Index (WQI) and analysis of variations of Water Quality Index
parameters we have taken average value of the parameters [3]. All the calculated
concentrations of water quality parameters in studied sampling sites showed variations. The
WQI plays significant role as a basis for environmental assessment and the value of these
samples ranges from 1-1000 which shows that water in all the sites are not within the suitable
mark for drinking. The analysis of Physico-chemical parameters indicates that the water in
the study area was polluted mostly by Aqua cultural inputs, industrial inputs, agricultural
wastes and domestic sewage from neighbouring villages which results in high concentration
of Nitrogen and Phosphorus [4]. Thus proper treatment is highly needed to conserve its water
body from future foulness, impurity and surrounding pollutions.
[1] Brahmaji Rao .P, Vijayalakshmi B.B R.G. (2017) Floral Diversity of Common Flora in
Kolleru Lake A.P IOSR Journal of Environmental Science, Toxicology and Food
Technology (IOSR-JESTFT) e-ISSN: 2319-2402, p- ISSN: 2319-2399.Volume 11, Issue
6 Ver. III (June. 2017), PP 01-11.
[2] Rachna Bhateria1, Disha Jain1, Water quality assessment of lake water: a review, J.
Sustain. Water Resour.Manag(2016)2:161-17
[3] ChungS.Y1,VenkataramananS1,Park.N2,Ramkumar.T3,Sujitha.S.B4,Jonathan.M.P4,
Evalution of physicochemical parameters in water and total heavy metals in sediments
at Nakdong River Basin, Korea, J. Environ Earth Sci (2016)75;50, 12665-015-4836-2
[4] Luharia N.M. et al., (2016) “Analysis and Seasonal Variation of Physico-Chemical
Parameters of Gawrala Lake and Vinjasan Lake of bhadrawati, district-chandrapur
(M.S.), india.”
OP-28: A New Stability- Indicating RP-HPLC method for the

determination of retigabine in oral dosage form
K.Swapna 1 , k.Ravindhranath2 ,Dr.D.Ramachandran3

1. Department of Chemistry,Bapatla College of Engeneering ,Bapatla, A.P India.
2. Acharya Nagarjuna University, Guntur,
Swapnakarnat.mmt@gmail.com;Ph.No. 9912461122
A new stability – indicating RP- HPLC method was developed for the determination
of retigabine in oral dosage form, using a ODS, C1s RP-Column (Make:250mmx 4.6 mm I.D:
particle size 5µm) and a mobile phase composed of phosphate buffer (PH -3.8) and
acetonitrile in the ratio of 55:45 %v/v at a flow rate of 1.0ml/min. The retention time of

(RACPABS - 2020)
retigabine was found to be 2.741 minutes, respectively. Retigabine was subjected to acid and
base hydrolysis, oxidation and photolytic degradation conditions and the degradation
products of retigabine were well resolved from the pure drug. This method can be
successfully employed for the quantitave analysis of retigabine in various formulations
respectively.
1) Bailer, M & White H., S. key factors in the discovery and development of new
antiepileptic Rundfildt C. The new anticonvulsant retigabine (D-23129) acts as an
opener of K- channels is newronal cells. Eur J chin pharmacol 1997:336:243- 249.
2) Meisel P.Landgraf K-F, Schafer J. Theif W.Rischer W. OLbrich A, et al. Us patent
(5.914.425) 1999.
3) PVV. Satyanarayana and Ajavala Siva Madhavi, New spectrophotometric methods for
the quantitative estimation of ezogabine in formulations, Ijrpc 2012, 2(4).
OP-29: STUDY OF MOLECULAR DOCKING FOR ISOLATED

BIOFLAVONOID COMPOUNDS
Dr.Chandrashekar.C1 and Dr.K Soujanya2

1. Department of Chemistry CMR College of Engineering & Technology (Autonomous)
Hyderabad-501401, India. Email:cchandrashekar@cmrcet.org
2. Department of Chemistry CMR College of Engineering & Technology (Autonomous)
Hyderabad-501401, India. Email:soujanya.kaki@cmrcet.org
Structure-based drug design plays a major role in the development of novel drugs
against many diseases. Recent advances in large scale determination of protein structures are
improving the drug discovery process by starting with the protein structure and using it to
design and identify new ligands. Insilco methods are routinely employed in order to achieve
high success rate and reduce the time taken for the process Quantitative structure–activity
relationship (QSAR), molecular modeling, molecular docking and pharmacophore modeling,
Literature survey reveals that rutin and many other bio-flavonoids exhibit anti-oxidant
activity by inhibiting the normal functioning of lambda-class glutathione transferases
(GSTLs), of particular xenobiotic inducible GSTLs, TaGSTL1 has been found to tightly bind
rutin, tafifolin, etc. The structures were drawn using Chem. Sketch 12 freeware, followed by
energy optimization by MMFF94 force field using TINKER. PyMol 1.7 and Avogadro 1
were used to calculate the molecular surface potential and to determine the intra-molecular
H-bonds. All the software's were used with default settings, operating in Linux Ubuntu 12.04.
1. Madsen, Ulf; Krogsgaard-Larsen, Povl; Liljefors, Tommy (2002). Textbook of Drug
Design and Discovery. Washington, DC: Taylor & Francis.

(RACPABS - 2020)
2. Leach, Andrew R.; Harren Jhoti (2007). Structure-based Drug Discovery. Berlin:
Springer.
3. .V.H.Masand, D.T.Mahajan, T.Ben Hadda, R.D.Jawarkar,H.Chavan,B.P.Bandar,
H.Chauhan, Molecular Docking and Quantitative Structure Activity Relationship
(QSAR) Analysis of Indoleylarylsulfones As HIV-1 Non Nucleoside reverse
Transcriptase Inhibitors, Med.Chem.Res., (2013).
4. H.V.Chavan,B.P.Bandgar,L.K.Adsul, V.D.Dhakane, P.S.Bhale, V.N.Thakare, V. H.
Masand, Design, synthesis, characterization and anti-inflammatory evaluation of novel
pyrazole amalgamated flavones, Bioorg.Med.Chem.Lett., 23(2013)1315-1321.
OP-30: DESIGN, SYNTHESIS, HYPOGLYCEMIC ACTIVITY AND

MOLECULAR DOCKING STUDIES OF 3-SUBSTITUTED-5-[(FURAN-
2-YL)-METHYLENE]-THIAZOLIDINE-2,4-DIONE DERIVATIVES
K. Srikanth Kumar*, Research Scholar, Krishna University, Machilipatnam, Andhra

Pradesh, India
A. Lakshmana Rao,V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Andhra
Pradesh, IndiaEmail: karumanchi002@gmail.com
Thiazolidine-2,4-dione was condensed with furan-2-aldehyde to form 5-[(furan-2-

yl)methylene]-thiazolidine-2,4-dione under Knoevenagel reaction conditions. Further it was
coupled with various alkyl/ aryl halides to afford the desired final derivatives 4a-4j. The
titled compounds were also prepared by microwave irradiation technique. All the compounds
were characterized physically and spectrally. The synthesized compounds were screened for
in vivo hypoglycemic activity. Molecular docking studies were carried out to find out the
significant binding interaction with PPARγ receptor.
Keywords: Thiazolidinedione derivatives, Synthesis, Hypoglycemic Activity, Molecular
Docking
KOH
Alcohol
S Piperidine Reflux 6 hrs S
S O
+ Toluene
O
O O OHC R-X O N O
N O O N O
H (4a-4j)
Thiazolidinedione Furfural H alkyl/ aryl halide R
5-[(furan-2-yl)methylene]-thiazolidine-2,4-dione
1. Gabriel, M., Ioana, I., Adrian, P., Laurian, V., Dan, C.V., Mihaela, D., Brindusa, T. and
Ovidiu, O. (2017), Microwave assisted synthesis of 3,5-disubstituted thiazolidine-2,4-
diones with antifungal activity. Design, synthesis, virtual and in vitro antifungal
screening, Farmica, 65(3), pp.414-422.

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2. Marc, G., Stana, A., Adrian Pirnau, A., Vlase, L., Vodnar, D.C., Duma, M., Tiperciuc,
B., Oniga, O. (2018), 3,5-Disubstituted thiazolidine-2,4-diones: design, microwave-
assisted synthesis, antifungal activity, and ADMET screening, SLAS Discovery, 23(8),
pp.807–814.
3. Shashikant, R.P., Prajact, K., Ashwini, P., Ana, N. and Kittur, B.S. (2009), Studies on
the Synthesis of novel 2,4-thiazolidinedione derivatives with antidiabetic activity,
Iranian Journal of Pharmaceutical Sciences, 5(4), pp.225-230.
OP-31: SYNTHESIS AND CHARACTERIZATION OF BARIUM

HYDROXIDE NANO PARTICLES
VeronicaDeekala1, Jyothsna Pragathi Yazala1, Anitha

Kowthalam1,RameshrajuRudraraju*1
Barium hydroxide nanoparticles were synthesized via chemical Co-precipitation

method from barium Nitrate and sodium hydroxide. Scanning electron Microscope and X-ray
diffractrometry were used to characterize the nanoparticles. The FTIR spectrum is used to
study the stretching and bending frequencies of molecular functional groups in the
sample.From Uv spectrum, the band gap of barium hydroxide nanoparticles is found to be
3.5ev.
OP-32: AN EXPERIMENTAL INVESTIGATION OF MOLECULAR

INTERACTIONS BETWEEN [EMIM][NTF2] IONIC LIQUID & 2-
ETHOXYETHANOL A COMPARISON BY PFP THEORY AND
COMPUTATIONAL STUDY
A. Sreenivasa Rao1,2 P.Sowjanya2 & B. Hari Babu2

1,2
Department of Chemistry, C.R. College, Chilakaluripet-522616, A.P., India.
2
Department of Chemistry, Acharya Nagarjuna University, Nagarjuna nagar–522510, A.P.,
India. e-mail (B Hari babu) dr.b.haribabu@gmail.com
Investigation of intermolecular interactions through hydrogen bonding has put on

much knowledge1,2 in the previous two decades and has been broadly investigated in both
investigational and theoretical/computational aspects3,4, many physicochemical properties
and thermodynamic processes can be influenced by intermolecular association.5-6Herein, to
investigate the intermolecular interactions in the binary of an IL, [EMIM][NTf2] with 2-

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ethoxyethanol(EE) as a function of temperature at atmospheric pressure, The properties such

as density (ρ), speed of sound (u) and refractive index (nD) were measured as a function of
temperature between (298.15-318.15) K at ambient pressure. These excess properties were
fitted to Redlich-Kister type equation for acquiring the coefficients in binary and standard
deviations. At the same time the MD simulations studies exhibit qualitative analysis of
hydrogen bonding between IL with the studied molecular solvent, additionally microscopic
interactions were also confirmed with ATR-FTIR spectra and Prigogine–Flory–Patterson
(PFP) theory was also supported the above interactions.
[1] G. R. Desiraju, Acc. Chem. Res., 35, 565(2002).

[2] L. Sobczyk, S. J. Grabowski and T. M. Krygowski, Chem. Rev., 105, 3513 (2005).
[3] C. L. Andersen, L. Du, and H. G. Kjaergaard, J. Phys. Chem. A, 118, 11074 (2014).
[4] Zapata-Escobar and A. Restrepo, J. Chem. Phys., 140, 184312 (2014).
[5] V. Znamenskiy, M.N. Kobrak, J. Phys. Chem. B. 108 (2004) 1072-1079.
[6] J.C. Lassègues, J. Grondin, D. Cavagnat, P. Johansson, J. Phys. Chem. A. 114 (2009)
687-688.
OP-33: BIOLOGICAL EVALUATION OF A BENZAZEPINE

DERIVATIVE 9-METHOXY-5-OXO-2,3,4,5-TETRAHYDRO-1H-
BENZO[B]AZEPINE-2-CARBOXYLIC ACID DERIVATIVES
Nagaraju Marepu, G.Mahesh, V.Santhoshi Sumana, M.Kanthi Kirani, and

Y.Sunandamma
Dept of Chemistry, Acharya Nagarguna University, Nagarjuna Nagar-522510, A.P., India.
Benzazepine is a heterocyclic compound constructed from fusion of a 7-membered

aliphatic ring with a benzene ring. Benzazepine nucleus has been used as the core structure
for various biologically active molecules and are generally used in the treatment of
hypertension, congestive heart failure and heart attacks. The vaptan class of ligands with the
1-benzazepine nucleus (e.g., lixivaptan, conivaptan and tolvaptan) have been developed as
agents for the treatment of hyponatremia and congestive heart failure. However, anticancer
properties of simple 1-benzazepine derivatives remained underexplored. In our recent
publication we have reported synthetic routes for Benzazepines with a mono chiral centre
non-peptide compound having arginine vasopressin (AVP) V2 receptor antagonist property.
Apart from anticonvulsant effect, (±)9-methoxy-5-oxo-2,3,4,5-tetrahydro-1H-
benzo[b]azepine-2-carboxamide, a derivative obtained from 9-methoxy-5-oxo-2,3,4,5-
tetrahydro-1H-benzo[b]azepine -2-carboxylic acid in our laboratory, is a potent anti-cancer
drug. i.e. IC50 of 173.14±0.61 μM. In line with the developments in the field of Benzazepine

(RACPABS - 2020)
and its derivatives, further studies are being carried out on 9-methoxy-5-oxo-2,3,4,5-
tetrahydro-1H-benzo[b]azepine-2-carboxylic acid by coupling with different aromatic and
aliphatic amines and screen the amine derivatives for anti-cancer and anticonvulsant
properties.
Synthetic scheme developed for 9-methoxy-5-oxo-2,3,4,5-tetrahydro-1H-
benzo[b]azepine -2-carboxylic acid.
OP-34: SIMPLE AND EFFICIENT SYNTHESIS OF SCHIFF BASE

DERIVATIVES OF 2-AMINOBENZOTHIAZOLE AND 2-
PICOLYLAMINE
Usharani.P 1 and N Madhavi*

1
Department of Chemistry, Acharya Nagarjuna University, Guntur*
*
Department of Chemistry, JKC College, Guntur, Andhrapradesh , India.
Email : madhavijkcchempg@gmail.com
Heterocyclic Schiff base derivatives were synthesized by the condensation of 2-

AminoBenzothiazole and 2-Picolylamine with substituted aromatic aldehydes in presence of
1eq of NaHCO3 and 2ml EtOH at 70oC for 5 hours. The structures of newly synthesized
Schiff base derivatives were confirmed by using FT-IR, 1HNMR and Mass spectral analysis.
Several 2-AminoBenzothiazole and 2-Picolylamine Schiff base derivatives were showed
Antimicrobial, Cytotoxic, Anthelmintic activities (1-4).
1. A.G. Awale , Dr. S. B. Gholse and Dr. P.S.Utale , IJSER, vol4, 1972-79 (2013).
2. Palani Venkatesh and Vijay Shankar Tiwari, Arabian Journal of Chemistry , vol9,
S914–S925 (2016)
3. Balaji P. N et al, Der Chemica Sinica, vol5 (1), 110-114, (2014).
4. Zahra Kazemi, Hadi Amiri Rudbari, Valiollah Mirkhani, Mehdi Sahihi, Majid
Moghadam,Sharam Tangestaninejad and Iraj Mohammadpoor-Baltork, Journal of
Molecular Structure, vol.1096, 110-120,(2015).

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OP-35: NOVEL FUNCTIONAL THIOPHENO AZOMETHINES AS

FLUORESCENT (TURN ON) AND COLORIMETRIC SENSOR FOR
THE CU2+ ION
Koteswara Rao Bandiab, Hongli Baoa*, Lic

a
Department of Chemistry, Ongole Campus, Rajiv Gandhi University of Knowledge
Technologies – A.P.
b
FJIRSM, Chinese Academy of Sciences, P. R. China
c
Fujian Normal University, P. R. China
*E-mail: dr.krbandi@gmail.com;Phone no.: +91-8919777670
Novel thiopheno based Schiff bases such as diethyl-2-amino-5-((7-(diethylamino)-2-

oxo-2H-chromen-3-yl)methylene-amino)thiophene-3,4-dicarboxylate [L1], diethyl-2-amino-
5-((7-methoxy-2-oxochroman-4-yl)methyleneamino)-thiophene-3,4-dicarboxylate [L2] and
diethyl-2-amino-5-(2-oxoacenaphthylen-1(2H)-ylideneamino)thiophene-3,4-dicarboxylate
[L3] were synthesized and characterized. The synthesized Schiff bases were screened to
construct an optical sensor. It was found that the ligand L1 is showing good affinity towards
Cu2+ ions. The colorimetric studies revealed that the ligand L1 is useful for the Cu2+
colorimetric sensor. As well as the ligand L1 is showing more selective fluorescent (Turn on)
behavior towards Cu2+ ion. The ligand L1 was successfully used for the naked eye
determination of Cu2+ ion.
Fig. 1. Colorimetric changes of diethyl-2-amino-5-((7-(diethylamino)-2-oxo-2H-chromen-3-

yl)methyleneamino)thiophene-3,4-dicarboxylate [L1] on the addition of two equivalents of
various metal ions (i.e., Cr3+, Mn2+, Fe2+, Co2+, Ni2+, Zn2+, Cd2+, Hg2+, Pb2+, Ag+, MeOH, L1
(2.5 × 10−1 mol L-1), Cu2+ (from left to right)).
[1]. E.I. Solomon, D.E. Heppner, E.M. Johnston, J.W. Ginsbach, J. Cirera, M. Qayyum,
M.T. Kieber-Emmons, C.H. Kjaergaard, R.G. Hadt, L. Tian, Chem. Rev. 114 (7) (2014)
3659– 3853.
[2]. K. Shen, Q. Wang, J. Am. Chem.Soc. 139 (37) (2017) 13110–13116.

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OP-36: DETECTION OF FENOPROFEN CALCIUM FORCED

DEGRADATION IMPURITIES BY UPLC‐QTOF MASS
SPECTROMETRY
P. Yella Reddy1, Ch. N. S .S Pavan Kumar1and S.Venkat Rao2

1
Vignan Vignan's Foundation for Science, Technology & Research(Deemed to be
University), VadlamudiGuntur-522213Andhra Pradesh
2
SMS R&D center (A DSIR Approved facility), Plot No. 189,190, SMS Pharmaceuticals
Ltd., Gagillapur (V), Hyderabad-500043, Telangana
This research article related to the separation, detection of proposedchemical

structures of the degradation impurities(DI) formed during degradation study of Feno profen
calcium (FPN) in its drug substance and as well as in drug product form by Ultra
performance liquid chromatography (UPLC) coupled with quadruple time‐off light mass
spectrometry (QTOF‐MS). The drug substance was subjected to stress conditions including
acid, base, thermal, oxidation, humidity and photolytic degradations. The resulting
Impurities and their fragment ions to be explore the elemental composition. Based on the
fragmentation pattern, the possible chemical structures of the unknown/known impurities
were proposed. To the best of our knowledge, there were no methods available to identify the
impurities during degradation of fenoprofen calcium by liquid chromatography‐mass
spectrometry.
DI1
DI2
O)2 Ca DI3
O O
DI4
FPN
DI5
DI: Degradation impurity
1. a) ICH Q1A (R2): Stability testing of new drug substances and products. Geneva,
Switzerland, 2003.b) ICH Q1B: Stability Testing: Photo stability Testing of New Drug
Substances and Products. Geneva, Switzerland, 1996.c) ICH Q2 (R1): Validation of
Analytical Procedures: Text and Methodology. Geneva, Switzerland, 2005.
2. ICH Q3A (R2): Impurities in New Drug Substances. Geneva, Switzerland, 2006.
3. ES Michael. J. Liq Chromatogr Rel Technol. 2005, 28 1253-1263.
4. J. Chem. Pharm. Res., 2017, 9(10):286-293

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OP-37: GREEN CHEMISTRY- THE ONLY WAY TO ECOLOGICAL

SECURITY
Dr.Ch. Udaya lakshmi1, Lecturer in Chemistry,Y.V.N.R.Govt. Degree college, Kaikaluru.

Dr. V.N.S.R. Venkateswararao2, Institute of aeronautical engineering (AIRE), Dundigal,
Hyderabad.
3
Smt. M.N.Parameswari , Lecturer in Chemistry,Y.V.N.R.Govt. Degree college, Kaikaluru.
Green Chemistry plays a significant role to combine the mechanical advancement

with ecological security is one of the key difficulties of the thousand years. Cleaner
technology is new measurement that is developing quickly at both national and worldwide
level. Cleaner production has been identified as a key method for reconciling environment
and economic development. The basic idea of cleaner production is to increase production
efficiency, reduce the use and generation of hazardous substances and by products etc. To
establish this cleaner production the various governments especially in cutting edge nations
like USA have been passed guidelines and laws. The chemistry in all over the globe are
motivated not only for environmentally begin synthesis of new products but also to develop
green synthesis for existing chemicals. This has been possible by the replacement of organic
solvent as much as possible, which are hazardous by water or eliminate the use of solvent
altogether. Essential thought of green chemistry is another methodology which gives the plan
of concoction items and procedures that limit the utilization and age of risky substances with
the goal that it is intrinsically of less hazard to human wellbeing and the earth. Engineered
scientific experts assume a key job to create green compound strategies to forestall the
contamination in the earth.
[1] A text book of Engineering Chemistry – II by Srinivasulu.D
[2] A text book of Engineering Chemistry by Dr.S.S.DARA, Dr. S.S. UMARE.
[3] Engineering Chemistry by Prasanta Rath.

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OP-38: SYNTHESIS AND CHARACTERIZATION OF SILVER

NANOPARTICLES USING GOMPHRENA SERRATA PLANT
EXTRACT
S. Pavani, Sk. Parveen, R. Sowmya, M. Rama Devi and K. Prasada Rao*

Department of Chemistry, Bapatla Engineering College, Bapatla-522101, A.P., India.
*email: prasad17467@gmail.com
The Nanoparticles, exhibit improved characteristics due to their size, distribution and
morphology and are widely used in numerous scientific fields [1]. Due to their
physiochemical and antimicrobial properties silver nanoparticles (AgNPs) are very important,
which help in therapies, molecular diagnostics and in devices used for medical procedures. A
major drawback of the chemical synthesis is that it involves the use of hazardous chemicals
and toxic by-products are obtained. Therefore, there is a constant need for economic and eco-
friendly methods to synthesize them and the use of aqueous or alcoholic plant extracts is
rapidly expanding and gaining importance [2]. Gomphrena serrata belongs to Amaranthaceae
family. The different parts of this plant are traditionally claimed to be used for the treatment
of broad spectrum of ailment including baby gripe, oligouria, body sore, malaria, bacterial
infections, jaundice, urinary problems, high cholesterol, cough, fever, diarrhea, liver
disorders, kidney disorders and cooling. A green synthesis of silver nanoparticles has been
done by using aqueous leaf extract of Gomphrena serrata [3]. The Synthesised silver
nanoparticles (AgNPs) were characterized with UV-Vis-spectroscopy, Fourier transform
infrared (FTIR) analysis and Scanning Electron Microscope (SEM) and it showed the
topology and size of the Ag-NPs, and X-ray diffraction studies. The screening of the silver
nanoparticles showed that it possess antimicrobial and antioxidant properties.
[1]. M.C. Daniel, D. Astruc, Chem. Rev. 104, 293, 2004.
[2]. O.V.Kharissova, H.V.R.Dias, B.I.Kharisov, B.O.Perez, V.M.J.Perez, “The greener
synthesis of nanoparticles”, Trends in Biotechnology, vol.31, no.4, pp.240-248, 2013.
[3]. Muhammad Ilyas, Arsia Tarnam and Nargis Begum Biological Potential and
Phytopharmacological Screening of Gomphrena Species, Global Journal of
Pharmacology 7 (4): 457-464, 2013

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OP-39: BIOANALYTICAL METHOD DEVELOPMENT AND

VALIDATION OF VENETOCLAX WITH INTERNAL STANDARD IN
PLASMA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
WITH MASS SPECTROMETRY: APPLICATION TO
BIOEQUIVALENCE STUDY

1. Dept. of Chemistry, Acharya Nagarjuna University, Guntur, AP, India.
2. Dept. of Chemistry, Andhra Layola College, Vijayawada, AP, India.
Bioanalytical methods employed for the quantitative determination of drugs and their
metabolites in biological matrix (plasma, urine, saliva, serum etc) play a significant role in
evaluation and interpretation of bioavailability, bioequivalence and pharmacokinetic data.
The present study is concerned with the development and validation of in vivo study of
Venetoclax in human plasma by high performance liquid chromatography /mass spectrometry
(LC/MS). Venetoclax is a BCL-2 inhibitor indicated for the treatment of patients with chronic
lymphocytic leukemia (CLL) or small lymphocytic lymphoma (SLL), with or without 17p
deletion, who have received at least one prior therapy. Sample preparation involves the
extraction of Venetoclax and internal standard Valsartan with liquid-liquid extraction
method by using methanol and water as an organic solvent. Chromatography was performed
on Prontosil ODS C18 column (4.6mm× 250 mm, 5 μm) under isocratic elution with mobile
phase of methanol and phosphate buffer (35:65 V/V). The mass spectra was taken in ESI
(Turbo Ion Spray) positive mode in mass range of 40–1000amu and analyzed in the
quadrupole analyzer with MS tune of 3.0 kV (capillary), 40V cone, 3V extractor and Source
temperature at 300oC. The method was found linear over the concentration range of 200ng to
10 μg/mL. The proposed method was validated for recovery, precision and lower limit of
quantification. Stability of compounds was established in a series of stability studies. The
validation results prove that accurate, specific and simple LC-MS method for estimation of
Venetoclax in plasma, using Valsartan as an internal standard. The method envisages that its
application to different biological matrices will facilitate deeper understanding and
demonstrated in the bioequivalence study.

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OP-40: DESIGN, SYNTHESIS AND MOLECULAR DOCKING STUDIES

OF eIF3a INHIBITORS AS POTENTIAL ANTICANCER AGENTS
Dr.M.Rajasekhar1,G.Mohini1,Dr.R.S.K.Sharma1and Dr.D.Ravi Kumar*

1
Department of Chemistry, GIET University, Gunupur-765022, Odisha, India.
*2
Department of Chemistry, Krishna University-Dr.MRAR PG centre, Nuzvid-521201,
Andhra Pradesh, India.;Email:sharmarsk178@gmail.com
Cancer one of the major issues globally and characterized by uncontrolled cell
proliferation. Hence, there is a necessity to develop target based anticancer agents. In this
study, we have synthesized pyridine-piperazine derivatives and evaluated their eIF3a
potentials using computational studies. Human eukaryotic Initiation Factor 3 (eIF3) is a
protein that consists of 13 non-identical subunits that help in the cap-dependent and cap-
independent translation initiation and also prevent premature association of the 40S and 60S
ribosomal subunits. eIF3a is the vast subunit of an eIF3 complex which is up-regulated in
many cancers like cervix, breast, lung, oesophagus, and stomach and is essential for cancer
growth and maintenance. Hence, the eIF3a could be regarded as molecular target for the
treatment of cancer. In computational and molecular dynamics simulations clearly indicated
that the synthesized molecules established critical interactions with protein. Therefore, these
derivatives would be the potential leads of eIF3a inhibitors.
OP-41: KINETICS AND MECHANISTIC STUDY OF OXIDATION OF

L-CITRULLINE BY PERIODATE IN ACIDIC AND BASIC MEDIA
D. Raga Sudha1, P. Vani2 , G. Nageswara Rao*2

1. Department of Chemistry, St. Joseph’s College for Women (Autonomous),
Visakhapatnam -530001, Andhra Pradesh, India.
2. Department of Inorganic and Analytical Chemistry, Andhra University,
Visakhapatnam-530003, Andhra Pradesh, India. Email: ragasudhadumpa@gmail.com
L-citrulline (2-amino-5-(carbamoylamino) pentanoic acid) is a naturally occurring

amino acid which is used for Alzheimer’s disease, dementia, fatigue, muscle weakness,
high blood pressure, and diabetes. It is also used for heart disease, body building,
increasing energy, and for improving athletic performance. L-citrulline might help
increase the supply of ingredients the body needs to making certain proteins. It is a key
intermediate in the urea cycle. In the body, L-citrulline is produced as a byproduct of the
enzymatic production of nitric oxide from the amino acid arginine, catalyzed by nitric
oxide synthase1. Periodic acid is one of the oxoacids of iodine. Periodate is a stable

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oxidizing agent in acid medium with redox potential 1.60V2 and in basic medium with
redox potential 0.70V3. Kinetics of oxidation of L-citrulline (Cit) by periodate ion in both
acidic and basic media has been investigated spectrophtometrically at constant ionic
strengths and at 30°C. In both media the reactions exhibited first order dependence in
[periodate] and fractional order dependences in L-citrulline concentration. A fractional-
second order dependence with respect to [H+] and a fractional-first order dependence with
respect to [OH-] were revealed in acidic and basic media, respectively. Increasing ionic
strength in basic medium increased the oxidation rate of L-citrulline, whereas it had a
negligible effect on the oxidation rate in acidic medium. The rate-determining step in both
media is suggested to involve a one-electron change. The proposed oxidation mechanisms
involve formation of 1:1 intermediate complexes between kinetically active species of
both L-citrulline and periodate ion in pre-equilibrium steps. The final oxidation products
of L-citrulline were identified in both acidic and basic media as the corresponding
aldehyde (4-(carbamoylamino) butyraldehyde), ammonia and carbon dioxide. The
appropriate rate laws are deduced. The activation parameters of the rate determining step are
computed using the linear least squares method.
Nos2 - Nitric Oxide Synthase". Uniprot.org. Uniprot Consortium. Retrieved 10 February
2015.
1. Latimer, W.M., ‟The Oxidation states of the Elements and their Potentials in Aqueous
solutions”, II Edn., Pretence-Hall,p.67(1952).
2. Giolitti, A., React. Acad. Lince., 14, 217(1905).
3. Nos2 - Nitric Oxide Synthase". Uniprot.org. Uniprot Consortium. Retrieved 10
February 2015.
OP-42: GREEN-CHEMISTRY (AIR POLLUTION)
G.Alluraiah
Dept. of Chemistry, S.V.Arts and Science College, Giddalur-523357,
Prakasam Dt, A.P , India.,
Air Pollution is the introduction into the atmosphere of chemicals, particulates, or

biological materials that cause discomfort, disease, or death to humans, damage other living
organisms such as food crops, or damage the natural environment or built environment. The
atmosphere is a complex dynamic natural gaseous system that is essential to support life on
planet Earth. Stratospheric ozone depletion due to air pollution has long been recognized as
threat to human health as well as to the Earth's ecosystems. Schematic drawing, causes and
effects of air pollution: (1) greenhouse effect, (2) particulate contamination, (3) increased UV
radiation (4) acid rain,(5) increased ground level ozone concentration, (6) increased levels of

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nitrogen oxides. A substance in the air that can be adverse to humans and the environment is
known as an air pollutant. Pollutants can be in the form of solid particles, liquid droplets, or
gases. In addition, they may be natural or man-made. Pollutants can be classified as primary
or secondary. Usually primary pollutants are directly produced from a process, such as ash
from a volcanic eruption, the carbon monoxide gas from a motor vehicle exhaust or sulphur
dioxide released from factories. Secondary pollutants are not emitted directly. Carbon
monoxide (CO)- is a colourless, odourless ,non-irritating but very poisonous gas. It is a
product by incomplete combustion of fuel such as natural gas, coal or wood. Vehicular
exhaust is a major source of carbon monoxide. Methane is an extremely efficient greenhouse
gas which contributes to enhanced global warming.
OP-43: SYNTHESIS OF NEW (±)-1-(4-(3-

FLUOROBENZYLOXY)PYRROLIDIN-3-YL)-4-PHENYL-1H-1,2,3-
TRIAZOLE DERIVATIVES VIA CLICK REACTION AND STUDY OF
ANTI-CANCER ACTIVITY AGAINST HCT 116, MDA-MB231, MIA-
PACA2 CELL LINES
Gollapudi Ravi Kumar1,2, Hari Babu Bollikolla1*

1
Department of Chemistry, AcharyaNagarjuna University, Nagarjuna Nagar-522510, Guntur,
2
Department of Chemistry, Government Degree College, Husnabad-505467,
Telangana, India.
A series of 16 new (±) -1-(4-(3-fluorobenzyloxy) pyrrolidin-3-yl)-4-phenyl-1H-1,2,3-

triazole derivative were synthesized from 2,5-dihydro-1H-pyrrole. Sixteen compounds are
well characterized by their 1H NMR, 13C NMR and mass spectral data. Anticancer activities
of these compounds were tested against HCT 116, MDA-MB231, Mia-PaCa2 cancer cell
lines. Among these series of compounds, 8b showed highest activity 42.5 µg/ mL against
MDA-MB231 cell line. The compound 8o and 8n show moderate activity 64.3 µg/ mL and
68.4 µg/ mL against the HCT -116 and Mia-PaCa2 cancer cell lines respectively.
Keywords: 1,2,3-Triazole, Anticancer, MDA-MB231, HCT 116, Mia-PaCa2.

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OP-44: REMOVAL OF HEALTH HAZARDOUS POLLUTANTS FROM

WASTEWATERS USING GREEN SYNTHESIZED NANOPARTICLES
Dr. M. Suneetha, Prof. B. Syama Sundar, Prof. K. Ravindhranath

1
Department of Chemistry, Rajiv Gandhi University of Knowledge Technologies-
Srikakulam, 2Department of Chemistry, Former Vice-Chancellor, Yogi Vemana University
3
Department of Chemistry, K. L. University, Vaddeswaram, Guntur Dist.
Corresponding author: drmsuneetha@rguktsklm.ac.in
The water pollution is attributed mainly to the presence of toxic metals (cadmium,
copper, lead, mercury, nickel, zinc, arsenic, chromium and aluminum), nutrients (phosphate,
nitrite, nitrate and ammonia), fluoride, organic dyes, cyanide, algae, viruses, bacteria,
parasites and antibiotics. The use of contaminated water sources contributes to the
transmission of water-borne diseases, including cholera, typhoid, diarrhoea,
Methaeglobinemia, fluorosis, hepatitis, trachoma, Parkinson and Alzheimer’s disease,
Minamata disease, etc. Hence, it is necessary to purge the waters. The nanoparticles play a
significant role in almost all the fields like biology, physics, chemistry, material science,
space industries, agriculture, environmental pollution, a variety of applications in the areas of
medicine, efficient and safe drug delivery mechanisms with less side effects, diagnosis and
treatment of various diseases (1). Nanoparticles synthesized by chemical methods have
unacceptable applications in all the fields because of the presence of toxic chemical
substances on the surface of the nanoparticles. Moreover, the cost effective, simple and eco-
friendly nature of green nanoparticles makes more attention on them. The different parts of
plant such as stem, root, fruit, seed, peel, leaves and flower are used to syntheses of metallic
nanoparticles in various shapes and sizes by biological approaches (2).Plant crude extract
contains several biomolecules such as enzymes, proteins, amino acids, phenolic acid,
vitamins, polysaccharides, citrates, flavonoids, alkaloids, terpenoids, ketones, aldehydes,
amides and carboxylic acids etc and these compounds are mainly responsible for the
reduction of ionic into bulk metallic nanoparticles formation (3).
Synthesis of metal nanoparticle in a plant extract (4)

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Green nanoparticles synthesized by using plant extracts are attracting the researchers in
the removal of health hazardous toxic pollutants present in surface and ground waters.
Different metallic nanoparticles prepared by using flora origin are effectively used to remove
organic and inorganic pollutants. The green synthesized nanoparticles are used in the
degradation of organic dyes, for the removal of inorganic solutes, toxic metal ions, natural
organic matter, complex organic compounds and other pollutants present in surface water,
ground water, and/or industrial waste water (5).
OP-45: BINARY MIXTURES OF ALLYL ALCOHOL WITH VARIOUS

FUNCTIONAL GROUPS(Thermodynamic and Acoustic properties)
Dheiver F. Santos; Email: dheiver.francisco@souunit.com.br

Engineering Department, Tiradentes University Center, Maceió, AL, Brazil
In the binary mixtures of Allyl alcohol (2-propen-1-ol) with various functional groups
(3-methyl aniline, 4-methoxy benzyl alcohol, 4-hydroxy benzoic acid and isobutyric acid) the
densities, and speeds of sound including those of pure liquids, were measured over the entire
composition range at different temperatures (303.15, 30.18 and 313.15) K and atmospheric
pressure 0.1MPa. Using this experimentally determined data, the excess values of molar
volume, isentropic compressibility, partial molar volumes and partial molar isentropic
compressibilities these components at infinite dilution were calculated. . The FT-IR data
indicate that the formation of intermolecular hydrogen bonding between hydrogen atom of
allyl alcohol and high electronegaivity of atoms various functional groups of non-component
molecules in the binary liquid mixtures. An analysis of each of the three contributions viz.
interactional, free volume and P* to VE shows that interactional contribution is positive for all
binary systems. The free volume effect and P* contribution are negative for all the mixtures

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OP-46: THERMODYNAMIC AND TRANSPORT PROPERTIES OF

BINARY LIQUID MIXTURES OF 2-ETHYL-1-HEXANOL WITH
KETONES AT VARIOUS TEMPERATURES
A. Venkatesulua*, M. Gowrisankarb
a
Postgraduate & Research Studies, Department of Physics,Govt. First Grade College,
Hosakote, Bangalore Rural, Karnataka,-562114, India; Email:
venkatesuluadavala@gmail.com
b
Department of Chemistry, J.K.C.College, Acharya Nagarjuna University, Guntur-522006,
AP, India Email: gowrisankar127@gmail.com
The densities (ρ), viscosities () and speeds of sound (u) are reported for binary
mixtures of 2-ethyl-1-hexanol with methyl ethyl ketone (MEK), methyl propyl ketone
(MPK), diethyl ketone (DEK) and 2,6-dimethyl cyclohexanone (CH) over the entire
composition range from 303.15 K to 318.15 K and at atmospheric pressure 0.1 MPa. The
excess / deviation functions (VE, κsE and ) are calculated from the densities, speeds of
sound and viscosities at experimental temperatures. The variation of these properties with
composition of the mixtures suggests hydrogen bond, dipole–dipole interactions and charge-
transfer complex formation between 2-ethyl-1-hexanol and dipolar ketones. The VE results
have been analyzed in the light of Prigogine-Flory-Patterson theory. An analysis of each of
the three contributions viz. interactional, free volume and P* to VE shows that interactional
contribution is positive for all binary systems. The free volume effect and P* contribution are
negative for all the mixtures.
OP-47: MOLECULAR INTERACTIONS BETWEEN OF ALLYL

ALCOHOL AND VARIOUS FUNCTIONAL GROUPS(BENZYL
CHLORIDE, 3-METHYLANILINE, 3-METHOXYANILINE AND 2,6-
DIMETHYLCYCLOHEXANONE)
B. Srikantha, D. Ramachandrana,*,
a
Department of Chemistry, Acharya Nagarjuna University, Guntur-522510, AP, India
In the binary mixtures of Allyl alcohol with various functional groups (benzyl
chloride, 3-methyl aniline, 3-methoxy aniline and 2,6-dimethyl cyclohexanone) densities,
speeds of sound and viscosities including those of pure liquids were measured over the entire
pressure 0.1MPa. Using this experimentally determined data, the excess  deviation

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parameters (molar volume, isentropic compressibility and deviation in viscosity) partial

molar volumes, partial molar isentropic compressibilities of these components at infinite
dilution were calculated. The results are discussed in terms the intermolecular forces between
the different component molecules in the binary mixture are more than the average
intermolecular forces existing between the similar molecules of pure components.
OP-48: EXPERIMENTAL AND THEORETICAL VALUES OF SOUND

SPEEDS AND VISCOSITIES FOR THE BINARY SYSTEMS OF 2,6-
DIMETHYLCYCLOHEXANONE WITH HYDROCARBONS
P. Venkateswarlu. pvchem0 l @mail corn

Department of chemistry, S. V. University, Tirupathi, A.P. India
Experimental data on excess volumes, speed of sounds and viscosities at 303.15 K are
measured for the binary systems of 2,6-dimethylcyclohexanone with hexane, heptane, octane,
2,2,4-trimethylpentane, cyclohexane and benzene over the whole range of composition. From
the experimental data density, isentropic compressibility, excess isentropic compressibility
and deviation in viscosity is calculated. The sound of speeds are evaluated theoretically on
the basis of Jacobson free length theory, Schaaffs collision factor theory and Nomoto theory.
Viscosity data are analyzed theoretically on the basis of corresponding states approach,
Grunberg–Nissan treatment and Mc Allister model. Furthermore, the experimental results are
discussed in terms of possible molecular interactions between unlike molecules.

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OP-49: THERMO PHYSICAL AND SPECTROSCOPIC PROPERTIES

OF BINARY LIQUID SYSTEMS: ACETOPHENONE/
CYCLOPENTANONE/ CYCLOHEXANONE WITH 3-
METHOXYLANILINE
R. Balajia, M. Chandra Shekarb

a
Department of Physics, Vignan Institute of Technology and Science, Deshmukhi-508284,
Telangana, India
b
Department of Physics, J.N.T. University, Hyderabad-500085, Telangana, India
Email: dr.m.chandrashekar@gmail.com
The intermolecular interactions in the binary liquid systems of 3-methoxyaniline with

acetophenone and cyclic ketones (cyclopentanone and cyclohexanone) are studied in
combined experimental and computational methodology. The speed of sound (u), density (ρ)
and viscosity ( ) values are measured for binary mixtures of 3-methylaniline with ketones
over the entire range of mole fraction at temperatures T= (303.15 to 318.15) K, at
atmospheric pressure. From the experimental results, the values of excess molar volume ( VmE
), excess isentropic compressibility (κsE), deviation in viscosity (η) and excess Gibbs free
energy of activation of viscous flow ( G *E ) are evaluated. The experimental results indicate
the intermolecular association between the binary liquid systems. Meanwhile, the natures of
hydrogen bond of the associated complexes are further confirmed by high level theoretical
calculations. The structures, interactional energies, and natural bonding orbital (NBO)
analysis of the hydrogen bonded complex have been calculated in the gas phase using density
functional theory (DFT) method at B3LYP/6-311++G (d, p) level.
OP-50: INFLUENCE OF LIGAND ON THE ACTIVITY OF METAL

IONS IN HETEROGENEOUS WATER-OXIDATION CATALYSTS
Satya Vijaya Kumar Nune

Centre of Excellence (CoExAMMPC), Vignan’s Foundation for Science, Technology and
Research – VFSTR (Deemed to be University), Vadlamudi – 522213, A.P., India
satyavijayakumar@gmail.com
Water splitting has been the prime focus in the research and development of
alternative energy sources. Water splitting comprises of two half reactions; water oxidation
and water reduction.
2H O → O + 2H E = 1.23 V @ pH 7

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Metal-oxide based catalytic systems were extensively investigated and reported for their
water-oxidation catalysis (WOC) properties.(1) Non-oxide catalysts, however, attracted
significant interest over the past few years. We have investigated a variety of non-oxide
catalysts including hexacyanometalates, pentacyanometalate derivatives, and other cyanide
ligand based catalysts for their WOC abilities.(2,3) Cobalt-based pentacyanoferrate and
dicyanamides were investigated previously for their WOC efficiencies. The focus of the
current research is to investigate the effect of the CN- ligand as bridging group between metal
ions, their influence on the catalytic activity, and surface concentration of the Co+2 ions.
(1) Nocera, D. G. Acc. Chem. Res. 2012, 45 (5), 767–776.

(2) Aksoy, M.; Nune, S. V. K.; Karadas, F. Inorg. Chem. 2016, 55 (9), 4301–4307.
(3) Nune, S. V. K.; Basaran, A. T.; Ülker, E.; Mishra, R.; Karadas, F. ChemCatChem 2017,
9 (2), 300–307.
OP-51: EFFICIENT SYNTHESIS OF 8-FLUORO-10-METHYL-3,4-

DIHYDROBENZO[B][1,6] NAPHTHYRIDINE-2(1H)-CARBOXAMIDE
AND THEIR CYTOTOXIC ACTIVITY
Rama Koteshwar Raoa and Shravankumar Kankala*a

a
Mewar University, Gangrar, Chittorgarh-312901, Rajasthan, India
*
Corresponding authors. Tel.: +91-9247461267;
E-mail addresses: shravankankala@yahoo.com (S. Kankala)
A facile synthesis of new series of 8-fluoro-10-methyl-3,4-dihydrobenzo[b][1,6]

naphthyridine-2(1H)-carboxamide hybrids in high yields has been developed via coupling
reaction. The synthesized naphthyridine-2(1H)- carboxamide hybrids were evaluated for in
vitro anti-cancer activity against a panel of four human tumor cell lines ie., MCF-7 (human
breast), A-549 (human Lung), OVACR-3 (human ovarian) and HeLa (cervical). The
compounds 12a, 12c, 12e, 12h and 12l demonstrate the activity higher than the positive
control.

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Synthetic approach to the substituted urea linked naphthyridine derivatives is out

lined in Scheme-1. Coupling of a 4 and 5 compounds in DIPEA, followed by addition of
Pdcl2(PPh3)2CH2Cl2 and cuI stirred at 70 oC led to 6 crude, this crude was reflux in mixture of
Methanol and Sulfuric acid gave pure compound 7, which reacts with 1-Boc-4-piperidone 8
in the presence of Sodium methoxide in THF gave to pure compound 9, followed by de-
protection of Boc group with TFA gave pure compound of 10 which was coupled with
different amines 11a-11l in mixture of ` affording the corresponding pure target compounds
12a-12l in high yield.
Scheme1.
2,2,2-trifluoro-1-(8-fluoro-10-methyl-1,2,3,4-tetrahydro-2,4-benzo[b][1,6]naphthyridine-
2-yl)ethan-1-one(10):

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OP-52: STUDIES ON CORROSION CHARACTERIZATION OF

ALUMINIUM 2024/ ALUMINIUM OXIDE METAL MATRIX
COMPOSITES IN HYDROCHLORIC ACID MEDIUM
R.Lakshmi1*, H.R.Radha1, P.V.Krupakara2 and Latha.V1

1
Department of Chemistry, T.John Institute of Technology, Bangalore.
2
Department of Chemistry, Cambridge Institute of Technology, North Campus, Bangalore
Karnataka.*Email: lakshmir@tjohngroup.com
Metal matrix composites are heterogeneous systems containing matrix and

reinforcement. Their physical and mechanical properties can be tailored according to
requirement. Metal matrix composites have emerged as advanced materials for several
prospective applications. They are used in automobile, aircraft and marine industries
because of their increased corrosion resistance. In this paper weight loss corrosion test is
conducted on Al2024/ Al2O3 metal matrix composites in different concentrated hydrochloric
acid solutions. Composites are prepared by liquid melt metallurgy technique using vortex
method. Composites containing 2, 4 and 6 percent of Al2O3 are prepared according to ASTM
standards. Specimens are machined and made ready by standard metallographic methods.
Weight loss corrosion studies are carried out in 0.025, 0.05, 0.075 and 0.1N solutions of
hydrochloric acid. The corrosion rate decreases with increase in the exposure time for all
specimens in all corrodents. Corrosion rate also decreases with the increase in reinforcement
content of the composites. Hence the composites can be used for the manufacture of the
equipments used in marine environment so that they lost long.
Keywords: Al2024/Al2O3 Metal, Corrosion characterization, Liquid Metallurgy Technique,
Metal Matrix Composites.

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OP-53: DEVELOPMENT OF MAGNETIC BASED METAL OXIDE AND

GRAPHINE OXIDE NANOCOMPOSITES FOR BIOLOGICAL,
CATALYTIC AND DIELECTRIC APPLICATIONS
D. Chandra Sekhar1,2, Bhagavathula S Diwakar1, N. Madhavi3*

1
Department of Engineering Chemistry, SRKR Engineering College, Bhimavaram-534204, India
2
Department of Chemistry, Acharya Nagarguna Univeristy, Guntur, India
3
P.G.Department of Chemistry, JKC College, Guntur-522006, India
Corresponding Author: madhavijkcchempg@gmail.com;
In this research work, the synthesis was aimed at the development of complex
nanocomposites, these materials were achieved through the chemical double co-precipitation
technique[1]. The materials were characterized by Powder X-ray Diffraction (PXRD),
Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Selected
Area Electron Diffraction (SAED), EDAX and Elemental mapping techniques. The
developed nanocomposites were further evaluated by vibrating sample magnetometer at room
temperature. Also, the nanocomposites were subjected to antimicrobial studies, catalytic
reduction of paranitroaniline, and dielectric studies by LCR meter. For silica coated samples
minimum inhibitory concentrations indicated that better antibacterial activity due to is porous
surface. For tin coated grapheme based samples exhibited better catalytic activity [2] and
dielectric nature [3] due to the availability of high surface area and comparatively better
dispersion on the GO surface which can be observed from the TEM images. However, this
type of GO based nanocomposites may find potential applications in water treatment
technologies due to the hydrophilic graphene oxide surface and they may also find potential
applications in microwave absorbing properties as well as in biological systems.
[1] D.C. Sekhar, B.S. Diwakar, N. Madhavi, Synthesis, characterization and anti-bacterial
screening of complex nanocomposite structures of SiO2@ZnO@Fe3O4 and
SnO2@ZnO@Fe3O4, Nano-Structures & Nano-Objects. 19 (2019) 100374.
https://doi.org/https://doi.org/10.1016/j.nanoso.2019.100374.
[2] B.F. Machado, P. Serp, Graphene-based materials for catalysis, Catal. Sci. Technol. 2
(2012) 54–75. doi:10.1039/C1CY00361E.
[3] Z.-S. Wu, G. Zhou, L.-C. Yin, W. Ren, F. Li, H.-M. Cheng, Graphene/metal oxide
composite electrode materials for energy storage, Nano Energy. 1 (2012) 107–131.
doi:https://doi.org/10.1016/j.nanoen.2011.11.001.

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OP-54: FRIED RICE SYNDROME- A COMPREHENSIVE

CONVENTIONAL AND ALTERNATIVE APPROACH TO
TREATMENT USING ADHATHODA VASICA
Dr. Swarna Latha Kurapati1, Chandra Mohan Kurmarayuni2,

Dr. Haribabu Bollikolla1*
1 Department of Chemistry, Ch.S.D.St. Theresa’s College for Women, Eluru, AP., India
1*, 2 Department of Chemistry, Acharya Nagarjuna University, N.Nagar, Guntur-522510,
AP., India;Email: kurapatisl1971@gmail.com
Bacillus cereus is a toxin-producing bacterium that is the main cause of food

poisoning, also called "fried rice syndrome. Fried rice is common food vehicles for Bacillus
cereus. If large quantity of food left out instead of wasting or rice which was cooked was kept
at room temperature for about 2 hours is cooled to make fried rice. The reheating of the rice
does not get freed from bacteria and leads to food poisoning. The silver nanoparticles
synthesized from Adhatoda vasica were shown more inhibition on Bacillus cereus which can
be utilized for the treatment of fried rice syndrome.
OP-55: ANALYTICAL METHOD VALIDATION FOR THE

DETERMINATION OF 2,6-DIAMINO-5-NITROPYRIMIDIN-4(3H)-
ONE IMPURITY IN 2-[(2-AMINO-1,6-DIHYDRO-6-OXO-9H-PURIN-9-
YL)METHOXY]-3-HYDROXYPROPYL
ESTER, MONOHYDROCHLORIDE DRUG BY UHPLC-MS/MS
Mahammad Ali Shaik1* Prof. Dr. N Devanna2

1.
Dept. of Chemistry, JNTU, Ananthapuram- 515002
2.
Dept. of Chemistry, JNTU, Ananthapuram-515002
A new and simple, rapid, selective isocratic liquid chromatographic method was
developed for the quantification of 2,6-diamino-5-nitropyrimidin-4(3H)-one impurity in the
drug,2-[(2-amino-1,6-dihydro-6-oxo-9H-purin-9-yl)methoxy]-3-hydroxypropyl ester, mono
hydro chloride by UHPLC-MS/MS. Good resolution was achieved between 2-[(2-amino-1,6-
dihydro-6-oxo-9H-purin-9-yl)methoxy]-3-hydroxypropyl ester, mono hydrochloride and its
related substance 2,6-diamino-5-nitropyrimidin-4(3H)-one by using Xselect, HSS cyano
(CN) (P/N: 186005945), 15 cm x 4.6mm, 5 µm column over a isocratic elution comprising
0.1% Ammonia solution in water and Acetonitrile in 50/50 v/v at 25°C column oven
temperature and sample compartment temperature 25°C. The USP tailing factor for 2,6-

(RACPABS - 2020)
diamino-5-nitropyrimidin-4(3H)-one impurity was found 1.0 and column efficiency Plate

counts (N) was found 9585. Mobile phase flow was fixed at a rate of 300 pL/min. The
method was validated as per ICH guidelines for accuracy, precision, specificity, linearity,
sensitivity Linearity was observed for 2,6-diamino-5-nitropyrimidin-4(3H)-one in the
concentration range of 0.235 – 1.173 ppm obtained correlation coefficient was 0.995.
Obtained accuracy data was from 104.4 to 112.5 from the range of 50% to 150% of the
sample concentration. The RSD for intra-day and inter-day precision was found to be less
than 7.7% and the sample solution was stable up to 24 hrs at 25°C. Validation studies
demonstrated that this UHPLC-MS/MS method is accurate, specific, rapid, reliable and
reproducible.
OP-56: SYNTHESIS AND SPECTRAL DETERMINATION OF ACTIVE

ANTIMICROBIALS OF PIPERIDINE ANALOGUES OF DIPHENYL-
BUTANAMIDES
Sriramudu. B, B. Satyanarayana, S. Venkat Rao, P. Bharath, K. Govinda Rao,

D.Ramachandran*
Acharya Nagarjuna University, N.nagar, Gunur-522510
A series of significant antimicrobial active analogs of N, N-dimethyl-4-(4-

Substituted-piperidine-1-yl)-2, 2-diphenyl-butanamide were synthesized with the
conservative chemical reactions like N-alkylation of 4th substituted piperidine precursors
with another key starting material 4-chloro-N, N-dimethyl-2, 2-diphenyl-butanamide. This
attempt of synthesis achieved successfully with different base and solvent combinations. The
compounds synthesized under this scheme were showing outstanding Antibacterial and
Antifungal activities against Bacillus cereus, Bacillus Subtilis, Escherichia coli,
Staphylococcus auveus, Vibrio paraheamolities, Klebsiella pneumonia, Bacillus magisterium,
and antifungal activity Candida albicans. Synthesized analogs are fundamentally related to
Loperamide, these consequences could helpful to derive more potential drug molecules.
R1
O N
N R2 K2CO3 R2
O N
C HN C
R1
CH3COCH3
Cl
1. Klaus, Profiles of Drug Substances, Excipients and Related Methodology, 1991,

Volume 19. Academic Press. p. 342.

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2. Dufek, Matthew B.; Knight, Beverly M.; Bridges, Arlene S.; Thakker, Dhiren R.
Drug Metabolism and Disposition: The Biological Fate of Chemicals. 2013, 41 (3): 642–
650.
3. Loperamide: voluntary withdrawal of infant formulations (PDF). WHO Drug
Information Vol. 4, No. 2, 1990, pp. 73–74.
OP-57: SYNTHESIS AND ANTICANCER EVALUATION OF

BENZIMIDAZOLE BOUND GLYCOSYL TRIAZOLE HYBRIDS
Vijay Kumar Allam, Brahmeshwari Gavaji*

b
Department of Chemistry, Kakatiya University, Warangal, India
Email: gbrahmeshwari@gmail.com
Benzimidazoles are widely present in amongst heterocyclic pharmacophores and

exhibit broad spectrum of biological properties like antibacterial, antiviral, antitumor and
anti-inflammatory. The early 1950s was an important period regarding discovery of the
biological significance of benzimidazole-containing structures and the closely-related
purines. The 5,6-dimethyl-1-(_-D-ribofuranosyl)benzimidazole ring system was discovered
in 1948 as the an integral part of the structure of vitamin B12. Benzimidazole ring is present
in important pharmaceutical, veterinary and agrochemical products such as thiabendazole,
cimetidine, azomycin, metronidazole, misonidazole, and chlotrimazole, antihistamines,
astemizole and the anti-ulcerative omeprazole). Here in we report the synthesis of some
new Benzimidazole- glycosyltriazole hybrids via 1.3-dipolar cycloaddition of N-Propargyl-2-
amino benzimidazole and various sugar azides. The products were characterized by NMR,
Mass and IR spectra. The anticancer screening of products was revealed that the hybrid
compounds were potent anticancer drugs than the standard drug Etoposide.
OH
OH
O
OH
N OH
N N N
K 2C O 3, D M F S u g a r A z id e s N
NH2 NH N
Br NH
N N C u S O 4 , N a A sc o rb a te
H H N
B u t O H : H 2 O ( 1 :1 )/6 h H
2 - A m in o b e n z im id a z o le
B e n z im id a z o le - g ly c o s y l tr ia z o le s .
Scheme: Synthesis of Benzimidazole bound glycosyl triazoles

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OP-58: THE STUDY OF INTERMOLECULAR INTERACTIONS IN {1-

BUTYL-3-METHYLIMIDAZOLIUM HEXAFLUOROPHOSPHATE + 1-
OCTYL-2-PYRROLIDONE} MIXTURE FROM VOLUMETRIC,
OPTICAL, ACOUSTIC AND SPECTROSCOPIC MEASUREMENTS.
V. SrinivasaRaoa,c, M. SrinivasaReddyb, B. HariBabuc*

a
Department of Chemistry, Govt. Degree College , Avanigadda 521121, A.P., India.
b
Department of Chemistry, Govt. Degree College , Kandukuru ,A.P., India.
c
Department of Chemistry, AcharyaNagarjuna University, Nagarjunanagar–522510,
A.P.,India,dr.b.haribabu@gmail.com
Densities, speeds of sound and refractive indices of [Bmim][PF6]+1-octyl-2-

pyrrolidone mixtures have been measured experimentally over the entire composition range
and at 303.15, 308.15, 313.15, 318.15 and 323.15 K, under 101.3kPa pressure. From these
E
experimental data, the excess molar volumes ( Vm ), excess values of partial molar volumes (
E
V m ), partial molar volumes at infinite dilution ( V mE , ), excess values of isentropic
E
compressibility (  sE ), free length ( Lf ), speeds of sound ( u E ) and isobaric thermal expansion
coefficient (  PE )have been evaluated. The computed data have been adequately fitted to the
Redlich-Kister polynomial equation. The measured and computed data have been used to
interpret intermolecular interactions between like and unlike molecules upon mixing. This
was further supported by IR spectroscopy and the Prigogine–Flory–Patterson (PFP) theory of
excess molar volume.
OP-59: REMOVAL OF FLUORIDE FROM DRINKING WATER BY

LANTHANUM (III) TREATED FLY ASH
PTSRK Prasada Rao

Department of Chemistry, P.B. Siddhartha college of Arts & Science, Vijayawada – 520010
e-mail : posanip@gmail.com
Fly ash is a major waste material generated due to the combustion of coal in power
producing plants. The composition of fly ash very considerably, depending upon the source
and make up of coal being burnt, however all fly ash contain substantial amount of silicon
oxide (SiO2,) Calcium oxide (CaO), aluminium oxide (Al2O3) and iron oxide (Fe2O3). As the
fly ash material solidifies while suspended in the exhaust gases, the particles of fly ash are
generally spherical in shape and ranges in size from 0.5m to 100m. The fineness of the fly

(RACPABS - 2020)
ash particles and the inherent large surface area (1-6m2g-1), together with the unburnt carbon
make it a good candidate for utilization as an inexpensive adsorbent7.
A Lanthanum (III) treated fly ash has been developed for the removal of fluoride from
drinking water by adsorption. The removal of F” by adsorption on La (III) treated flyash was
found more than 80% at neutral pH,, (F”) 20 ppm, adsorbent dose 2 g per litre, contact time
30 min and temperature 30 + 20C. An improved adsorption was find in the pH range 5.2 to
7.30 and the optimum pH for adsorption was found 50. The fluoride ions are mainly removed
by ion exchange process with surface hydroxyls however the mechanism of adsorption
appear to be complex. The data fitted well in Freaundlich adsorption is a therm. The values of
adsorption constants K and 1/n were found as 0.29 and 0.64 respectively.
OP-60: RP-HPLC METHOD FOR THE DETERMINATION OF

RANOZALINE IN PHARMACEUTICAL FORMULATIONS.
Masma Shaik, G.Mahesh, V.Santhoshi Sumana, M.Kanthi Kirani, and Y.Sunandamma

Dept of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, A.P., India.
A simple, sensitive and accurate method through reverse phase high performance
liquid chromatography was developed for determination of Ranozalinein in pharmaceutical
formulations. Excellent separations were achieved on a Thermo Hypersil-BDS C-18,
250x4.6mm column with particle size of 5µm. A suitable mobile phase of a mixture of buffer
and acetonitrile in the ratio of 50:50 v/v used. The flow rate of mobile phase was 1.0 mL/min.
and the total elution was for 9 min. The UV-detector wavelength was at 220 nm and the
temperature was maintained at 32oC. Method of determination was validated in terms of
system suitability, specificity, accuracy, precision and robustness for quantification of
ranozaline following the ICH-guidelines.

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OP-61: AN EXPERIMENTAL STUDY ON MICROBIAL POWER

MODULE FOR WASTE WATER TREATMENT AND ELECTRICITY
GENERATION
Dr. D. RamaChandran, Assoc.Prof., Acharya Nagarjuna University, Guntur

Mr. T. Benjamin, Noble college, Machilipatnam
Dr. Ch. Rajyalakshmi, Assoc. Prof. of Chemistry, Vishnu Institute of Technology,
Bhimavaram Email: rajyalakshmi.ch@vishnu.edu.in
A Microbial power module (MPM) is a rising innovation. It is a bioreactor that

converts chemical energy in organic compounds to electrical energy through catalytic
reactions of microorganisms under anaerobic conditions. Electrical energy generated from
microbial power module could be used for small electrical device such as biosensors etc.
Anode, cathode, salt membranes are fundamental segments of microbial power module. It
contains two chambers to isolate terminals from long separation for creation of power,
utilization of waste water in anaerobic chamber offices development of the local
microorganisms. Including substrates increment efficiency the electrons are move from anode
chamber to cathode chamber by salt scaffold. Bioreactors dependent on power age in power
module may speak to a totally new way to deal with wastewater treatment. If it is optimised,
MPM’s would prove to be new method to offset wastewater treatment plants operating costs.
Extensive research on MPMs is going on at laboratory scale but very few pilot scale studies
have been reported. This paper is an attempt to study the three different wastewater samples.
The three diverse wastewater tests utilized were Activated sludge from sewage plant, local
waste water and leachate from food waste. The most noteworthy pace of voltage age is
accomplished when the MFC was worked with leachate followed by activated sludge. In any
case, in view of the investigation of the diagram design created, Activated sludge gave the
most predictable record as far as power age. The most elevated productivity of COD
evacuation is accomplished by Activated sludge. The Activated sludge has likewise
demonstrated the most elevated proficiency as far as nitrogen expulsion followed by leachate.
The results of the experimental study are promising and encouraging for further scaling up of
MPMs.
1. Sanjay S, Sowmyashree D, Udayashankara T H, Treatment of Dairy Wastewater and
Bioelectricity Generation Using Membraneless Microbial Fuel Cell , International Journal of
Civil Engineering and Technology (IJCIET) Volume 9, Issue 5, pp. 679–685, May 2018.
2. Jessica Li , An Experimental Study of Microbial Fuel Cells for Electricity Generating:
Performance Characterization and Capacity Improvement , Journal of Sustainable Bioenergy
Systems, 3, 171-178, 2013.
3. Pawandeep Singh Dhingra, Shubham Shelke, S. M. More, Generating Electricity with
Microbial Fuel Cell (MFC), International journal of Advance Research in Science and
Engineering, Volume 7, issue 2, February 2018.

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OP-62: SYNTHESIS OF NEW DERIVATIZEDDITHIZONELIGANDS

AND ITS USE IN THE ESTIMATION OF MERCURY FROM WASTE
WATER SAMPLE USING UV-VIS SPECTROPHOTOMETRY
A.Tejaswini* and P.V.Krupakra

Department of Chemistry, Cambridge Institute of Technology-North campus,
Bangalore – 562110;*Email: tejaswinianumula@gmail.com
Industrialization and Urbanization has lead to the use of heavy metal ions like
Mercury, Lead, and Cadmium, these are released as effluent and enter in aquatic systems
from which it enter into the food chain and start accumulating in human body.
Spectrophotometric method is found to be simple, cost effective method but with moderate
sensitivity. Dithizone is a standard and extensively used chromogenic reagent in estimating
Mercury ions. In this work, p-methoxy aniline was diazotized and reduced to obtain p-
methoxy phenyl hydrazine further condensing with CS2 and KOH to obtain compound (4).
Similarly p-methoxy phenyl hydrazine was reacted with 3,4,5-trimethoxyphenylisothi
ocyanate and p-methoxyphenylisothiocyanate followed by oxidation using N-
BromoSuccinimide to obtain compound(7 &10). The synthesised compound was used as
ligand for the estimation of Hg+2 using colorimeter and UV-Vis spectrophotometer. We
didn’t observed any prominent colour or change in UV absorbance with respect to the blank
solution. Further modification in the structure of ligands and experimental parameters are to
be conducted in order to study the complexation behaviour of Mercury with synthesized
ligands.
OP-63: COPPER MEDIATED SELECTIVE HALOGENATION OF

ARYLCYANAMIDES: SYNTHESIS OF ORTHO HALO
ARYLCYANAMIDES
S N Murthy Boddapati,a,b Chandra Mohan Kurmarayunia, Hari Babu Bollikolla*a
a
Dept. of Chemistry, Acharya Nagarjuna University, Guntur, Andhar Pradesh, India-522510.
b
Dept. Of Chemstry, Sir C. R. Reddy College, P G Courses, Eluru, Andhra Pradesh, India-
534002.Email: dr.b.haribabu@gmail.com
A novel, efficient and selective route for the synthesis of various substituted
2- halo arylcyanamides via C-H functionalization of arylcyanamides using a cheap, air stable
and readily available copper source as catalyst is achieved. The present method involves a
selective ortho halogenation strategy. Moreover, the reaction mechanism has also been
investigated. The reported protocol is simple, general with excellent substrate tolerance in
good to high yields thus paving an efficient and useful way to establish a library of 2- halo
arylcyanamides.

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OP-64: COBALT(II) COMPLEXES OF PYRIDYL TETRAZOLE

LIGANDS WITH PENDANT ARMS AMIDE AND HYDRAZIDE -
SYNTHESIS, CHARACTERIZATION, DNA BINDING STUDIES
Shaik Mustafa* and Syed Khasim sharif

Department of Chemistry, Tagore Govt. Arts and Science College, Lawspet, Puducherry-605
008 E-mail: mustaff_02@yahoo.co.in
New pyridyl tetrazole ligands 2-(5-(pyridin-2-yl)-1H-tetrazol-1-yl)acetamide (L1), 2-

(5-(pyridin-2-yl)-2H-tetrazol-2-yl)acetamide (L2), 2-(5-(pyridin-2-yl)-1H-tetrazol-1- yl)aceto
hydrazide (L3) and 2-(5-(pyridin-2-yl)-2H-tetrazol-2-yl)acetohydrazide (L4) have been
prepared. These ligands have been coordinated with CoCl2.6H2O to furnish corresponding
complexes [Co(L1)2]-[Co(L4)2]. Electro paramagnetic resonance spectra of all the Cobalt
complexes are characteristic of square planar geometry, with nuclear hyperfine spin 3/2.
DNA- binding studies using UV–Vis absorption spectroscopy, viscosity and thermal denature
studies revealed that all of these complexes are avid binders of calf thymus DNA. The
antioxidant properties of the free ligands and the Co(II) complexes were investigated using
the DPPH radical scavenging method, and [Co(L4)2] was found to show thehighest activity.
OP-65: Synthesis And Characterisation Of Some New Hydrazone

Derivatives Of 3- (2-Hydroxyl-5-Methyl Phenyl)-3- Phenyl Propane)
Ramya Krishna Pallapati1 and Dr. B. Hari babu*1

1
Department of Chemistry, Acharya Nagarjuna University, Guntur-522 510, Ap., India.
Email : dr.b.haribabu@gmail.com and ramyapallapati@gmail.com
Some new analogues of 3- (2-hydroxyl-5-methyl phenyl)-3- phenyl propane) derived

hydrazide-hydraones were produced by using various structurally divergent hydrazide-
hydrazones were obtained in moderate to good yields in methanol at 45-85 °C for 4-8 hr
stirring. Out of all aromatic benzaldehydes, substitutions with 4-methyl exhibit good yield
(81.5%) compared with 4-ethoxy and 4-trifluoro methyl 80.2 and 78.5% respectively. These
derivatives exhibit good antibacterial activities.
OH
OH
H
N NH2 H
a N N CH
H3C
H3C
O
O
R
2a : 4-CH3
2b : 4-CF3
2c : 4-OCH3

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a: Methanol, at 0-15 °C, and 45-85 °C for 4-8 hrs.

1. Belkheiri N, Bouguerne B & Belval F, Eur J Med Chem, 45, 2010, 3019.
2. Almasirad A, Tajik M & Baktiari D, J Pharm Sci, 8, 2005, 419.
3. Sridhar S K, Pandeya S N, Stables J P & Ramesh A, Eur J Pharm Sci, 16, 2002, 129.
4. Salgin U, Oksen G, Okhan-Kelekci N G & Oktasl O G, bioorg Med Chem, 15, 2007,
5738.
OP-66: 3-[(3-(TRIMETHOXYSILYL)PROPYL)THIO]PROPANE-1-
OXY-SULFONIC ACID: AN EFFICIENT RECYCLABLE
HETEROGENEOUS CATALYST FOR THE SYNTHESIS OF 1H-
PYRAZOLO[1,2-B]PHTHALAZINE-5,10-DIONE DERIVATIVES AND
THEIR BIOLOGICAL EVALUATION
Srinivasa Rao Jetti,*1 Shubha Jain2

1
Department of Chemistry, Narasaraopeta Engineering College, Narasaraopet,
Andhra Pradesh-522601, India
2
School of Studies in Chemistry & Biochemistry, Vikram University, Ujjain,
Madhya Pradesh-456010, India
*Corresponding author: srinujetti479@gmail.com
A rapid and efficient one-pot three-component protocol for the synthesis of 1H-
pyrazolo [1,2-b] phthalazine-5,10-diones has been developed by domino coupling of
phthalhydrazide, 1,3-diketones, and aldehydes catalyzed by 3-[(3-(trimethoxysilyl) propyl)
thio] propane-1-oxy-sulfonic acid. The structures of all synthesized compounds have been
established by spectral and elemental analysis. All synthesized compounds have been
evaluated for their antibacterial, antifungal and antioxidant activity.
Keywords: 3-[(3-(trimethoxysilyl)propyl)thio]propane-1-oxy-sulfonic acid, Heterogeneous
Catalysis, 1H-pyrazolo[1,2-b]phthalazine-5,10-diones.
O
Ar
O
Ar CN
TMSPTPOSA N
NH
X
NH Ethanol, Reflux N
O H X
O NH2
X = CN, COOEt O

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1. Xu, Y.; Guo, Q‐X. Heterocycles 2004, 63, 903‐974.

2. Li, J.; Zhao, Y.F.; Yuan, X.Y.; Xu, J.X.; Gong, P. Molecules 2006, 11, 574‐582.
3. Srinivasa Rao Jetti, Amitbodh Upadhyaya, Shubha Jain. Medicinal Chemistry Research,
2014, 23(10), 4356-4366.
OP-67: NEW POLYMERIC COPPER 2-PICOLONICACID CRYSTAL

STRUCTURES AND ITS BIOLOGY
Dr. K.Mohana Rao1 Dr. B.Kishore Babu2 S.V Venkat Rao1

1
Department of Chemistry RGUKT-Nuzvid Krishna
2
Department of Engineering Chemistry, AUCE (A), VSKP
mohaneusi03@gmail.com, 9502814852
In modern years, chemists have tended to plan and synthesis metal-organic frameworks,
which are undergoing accelerated and continuous growth because of their fascinating
structures, and potential applications, such as molecular adsorption, catalysis,gas storage,
multifunctional materials, and chemical separation 2-picolinic acid is not only a potential
proton acceptor but also proton donor depending on deprotonated groups [1-2]. To the best
of our understanding the Pyridine,2-carboxaldehyde into 2-picolinic acid (scheme 1). Lattice
water molecules from the metal salt are trapped in the Cu-picolinate network lattice forming a
1-D water chain and playing a major role in formation of the H-bonded coordination network.
In this crystal engineering of copper picolinate complex formed by in situ oxidation of
pyridine-2-carboxaldehyde with copper (II) perchlorite is discussed. The carboxylates ions
not only take part in coordination to metal but also provide hydrogen bond acceptor sites for
lattice water. The synthesis, single-crystal X-ray structure, FTIR, luminescence and Biology
of a new Cu (II)-picolinate coordination complex is reported.
1. Li, Y.W., Tao, Y and Hu, T.L. Solid State Sciences.2012, vol.14,8,1117–1125.
2. Colacio, E., Maria,A., Palacios, A.R. AntonioRodrguezDieguez, Inorganic Chemistry.
2010, vol.49, 4, 1826–1833.
3. unay, G., Yes¸O.Z., ilel,M., Soylu,S., Keskin,G and Dal, H. Synthetic Metals. 2011,
vol.161, 21-22, 2471–2480.

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OP-68: SYNTHESIS AND CHARACTERIZATION OF SOME NEW

IMIDAZOLEBENZOHYDRAZIDE DERIVATIVES.
Mannem Gangadhara Rao, Navudu Ramesh and B. Hari Babu*

Department of Chemistry AcharyaNagarjuna University, Nagarjuna Nagar-522510
Mail: dr.b.haribabu@gmail.com
A string of new Imidazolebenzohydrazide derivatives were synthesized starting from

Imidazolecompound(1) in three consecutive steps. Step one involves N-alkylation of
imidazole compound with chlorocompound(2)in acetone and potassium carbonate.Stepis two
azide (4) formation with hydrazine hydride in ethanol and final Step continues with various
aldehydes (5a-5n)condensationat room temperature in ethanol. Synthesized a total of 14
newImidazolebenzohydrazide derivatives (6a-6n) and all the synthesized compounds were
characterized by 1HNMR ,13C NMR and Mass spectral analysis.
O
OC2H5
O
OC2H5 N K2CO3 N
Cl N OH
HN + N
OH N N
Acetone
N
1 2 3
EtOH NH2NH2
Ar
N O
O NHNH2
NH ArCHO
5a-5n N
N N OH
N OH N N
N N
6a-6n
OP-69: ANTIMICROBIAL ACTIVITIES OF SOME ARYL

CYANAMIDES AND ARYL TETRAZOLEAMINE DERIVATIVES
Nathani Srinivasa Raoa and Kondraganti Lakshmib

a
Department of Chemistry, Bapatla Engineering College, Bapatla,
Andhra Pradesh, 522 102, India
b
Bapatla Municipality, Women Protection Secretary, Bapatla,
Andhra Pradesh, 522 101,India
The emergence of new infectious diseases, the resurgence of several infections that
appeared to have been controlled and the increase in bacterial resistance have created the
necessity for studies directed towards the development of new antimicrobials[1-2].
Considering the failure to acquire new molecules with antimicrobial properties from
microorganisms, the optimization for screening methods used for the identification of

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antimicrobials from other natural sources is of great importance. This study was carried out
with an objective to investigate the antibacterial and antifungal activity of aryl cyanamides
and aryl tetrazoleamines. The aim of the study is to assess the antimicrobial activity and to
determine the zone of inhibition of extracts on some bacterial and fungal strains. In the
present study, the microbial activity of aryl cyanamides and aryl tetrazoleamines was
evaluated for potential antimicrobial activity against medically important bacterial and fungal
strains. The antimicrobial activity was determined in the extracts using filter paper disc
diffusion plate method [3] The antibacterial and antifungal activities of extracts (40 μg/ml) of
aryl cyanamides and aryl tetrazoleamines were tested against Bacillus cereus (MTCC 430),
Staphylococcus aureus (MTCC 3160), Pseudomonas aeruginosa (MTCC 424),and
Escherichia Coli (MTCC 40) Zone of inhibition of extracts were compared with that of
different standards like ampicillin, and norfloxacin, for antibacterial activity and griseofulvin
for antifungal activity. The results showed that the remarkable inhibition of the bacterial
growth was shown against the tested organisms.
1. D. S. Arora, Singh, J. and Aneja, K. R (1999) Some Indian spices and their
antimicrobial properties. Pp. 33-40. In:. (Eds.). From Ethnomycology to Fungal
Biotechnology: Exploiting Fungi from Natural Resources for Novel Products. Kluwer
Academic/Plenum Publishers, New Yok
2. Qiong Chen, Xiao-Lei Zhu, Li-Li Jiang, Zu-Ming Liu, Guang-Fu Yang (2008)
Synthesis, antifungal activity and CoMFA analysis of novel 1,2,4-triazolo[1,5-
a]pyrimidine derivatives. European Journal of Medicinal Chemistry 43, 3,595-603
3. Bonev, B., Hooper, and J.,Parisot, J. (2008). "Principles of assessing
bacterialsusceptibility to antibiotics using the agar diffusion method". The Journal of
Antimicrobial Chemotherapy. 61, 1295–301.
OP-70: STABILITY INDICATING ASSAY METHOD DEVELOPMENT

AND VALIDATION OF NETARSUDIL AND LATANOPROST BY RP-
HPLC AND ITS DEGRADATION
M.Manoranjani
Department of Chemistry , P.B.Siddhartha College of Arts and Science,Vijayawada
An accurate, rapid economical and simple, reliable method was developed and
validated for the simultaneous estimation of Netarsudil and Latanoprost using RP-HPLC. In
the proposed method efficient chromatographic separation was achieved using X-Bridge
Phenyl column (150x4.6mm, 3.5μm) as a stationary phase and acetonitrile: buffer (30:70v/v,
2.5g of Octane-1-Sulphonic acid in 1lt of water adjust pH-2.5 with Ortho Phosphoric Acid) as
a mobile phase with a flow rate of 1ml/min and UV detection at 228nm. Chromatography

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was carried out isocratically at ambient temperature and the run time was approximately
10min. In this method a good linearity was observed in the range of 0.0063-0.0938μg/ml and
0.0013-0.0188μg/ml with the limit of detection (s/n=7, 4) respectively. The proposed method
was validated as per ICH guidelines.
OP-71: EFFECT OF PH, COPPER SULPHATE AND VITAMIN E ON

SEED GERMINATION OF BLACK GRAM AND BENGAL GRAM
V. Ravi1, B.Sai kiran2, B.Ajay kumar3, N Madhavi4 *

P.G. Department of Chemistry, J.K.C. College, Guntur, Andhra Pradesh, India.
The present study deals with effect of pH,copper sulphate and vitamin E on in vitro
germination of black gram and bengal gram. At pH range of 2.5 to 12 the seeds are
germinated .Highest seed germination is observed at pH 8.0. Poor germination is observed at
pH 2.5 and 1.2. The increase in concentration of copper sulphate retarded the germination
rate .But with the addition of vitamin E ,enhanced seed germination is observed.This
investigation confirms that it is preferable to choose pesticides without copper in their
composition and presoaking of these seeds in vitaminE solution increases the seed
germination rate.
1. Chatterjee J, Chatterjee C,2000, Phyto toxicity of cobalt,chromium and copper in
canliflower, Eniviron.Pollut.109:69-74.
2. Sing D, Nath K, Sharma YK, 2007, Response of wheat seed germination and on
seedling growth under copper stress, J.Environ.Biol. 28(2),409-414.

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OP-72: AN ANALYTICAL METHOD DEVELOPMENT AND

VALIDATION FOR THE DETERMINATION OF 4,6 DICHLORO 5-
NITRO-2-(PROPYLTHIO)PYRIMIDINE IN TICAGRELOR DRUG
SUBSTANCE BY LC-MS
Krishna Moorthy Manchuri1 Gopi Reddy Venkata Subba Reddy2

1. Department of Chemistry, JNTUA, Ananthapur-515002, Andhra Pradesh, India.
2. Department. of Chemistry, JNTUA College of Engineering, Pulivendula,
Kadapa-560 390, Andhra Pradesh, India.
A novel selective and sensitive liquid chromatography coupled with mass

spectrometric (LC-MS) method was developed and validated for the determination of 4,6
dichloro 5-nitro-2-(propylthio)pyrimidine in Ticagrelor drug substance. The method utilizes
Chromolith RP-18 endcapped column (100 mm×4.6 mmx1.2 µm) with positive ionization in
multiple reaction monitoring (MRM) detection mode. An isocratic program developed for
rapid analysis and mobile phase selected as a pH 3.5 Ammonium formate and acetonitrile in
the ratio of 15:85(v/v). The flow rate of 0.7mL/min used and elution monitored through mass
spectrophotometer. The method validation performed according to current International
Conference on Harmonization (ICH) guidelines for an impurity: 4,6 dichloro 5-nitro-2-
(propylthio)pyrimidine with validation parameters of accuracy, precision at LOQ, limit of
detection, limit of quantification, specificity, linearity and sensitivity. A linearity performed
in the concentration range of 2.4 to 12.2 ppm (Limit of quantification to 150% of the
specification limit) and obtained correlation coefficient is 0.999. An accuracy performed
from the range of 50% to 150% of the specification limit and an obtained accuracy is between
80% to 120%. All the method validation parameters demonstrated that this method is
accurate, precise, specific, linear and reproducible.
OP-73: SYNTHESIS AND ANTI-DIABETIC ACTIVITY EVALUATION

OF SOME NEW THIAZOLIDINEDIONE DERIVATIVES
Ch. Ramya, P. Lakshmi Sowjanya, V. Yugandhar and Dr. Ch. Subramanyam*

Department of Chemistry, Bapatla Engineering College, Bapatla - 522101,
India.Corresponding author: Email: *subbusree04@gmail.com, 9490385962
Type 2 diabetes mellitus (T2DM), is a quick progressing sickness worldwide; is one

of the fundamental motive of deaths.[1] Inhibition of saccharide hydrolyzing enzyme (α-
amylase) have been useful as oral hypoglycemic drugs for the control of hyperglycemia
mainly in patients with T2DM. Inhibition of this enzyme hold-up carbohydrate digestion and

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extend overall carbohydrate digestion time, causing a decrease in the rate of glucose
absorption and, therefore, reducing the postprandial plasma glucose rise. Heterocyclic
compounds are the basis in anti-diabetic treatment for many years. Thiazolidinediones are
well known for causing reduction in blood glucose levels. A number of thiazolidinediones
have been approved for clinical use in diabetes. However many TZD based drugs are proved
to be oral antidiabetic drugs, hepatotoxicity is one of the serious side effects of these drugs.
Hence there is a need of novel drug with better safety profile.[2] On the basis of previous
literature and present want of developments on the synthesis of novel anti-diabetic drugs and
in extension of our studies to develop new anti-diabetic agents; we have synthesized some
new TZD derivatives. The newly synthesized compounds were characterized by NMR (1H
and 13C), IR spectra. In vitro anti-diabetic activity of synthesized compounds was examined
by standard α-amylase inhibition assay. Some of the synthesized compounds exhibited good
activity when compared with the standard drug.
[1]. Rang, H.P., Dale, M.M., Ritter, J.M., Flower, R.J., 2008. Pharmacology, sixth ed.
Elsevier Science, London.
[2]. Prasanna A. Datar, Sainath B. Aher, Journal of Saudi Chemical Society (2016) 20,
S196-S201
OP-74: ANALYTICAL METHOD DEVELOPMENT AND

VALIDATION FOR THE DETERMINATION OF LUMACAFTOR
USING RP-HPLC
Syed. Ibrahim Baje1,M.L.N.Raj Gopal2, G.Praveen Chand3, N Madhavi4 *.

2
P.G. Department of Chemistry, T J.P.S. College, Guntur, Andhra Pradesh, India.
1,3,4
A simple, accurate and precise method was developed for the estimation of
Lumacaftor in liquid dosage form. Chromatogram was run through Kromocil 100 C18, 150
x 4.6 mm, 3.5. Mobile phase –A containing dilute 1.5 ml of ortho phosphoric acid in 1000
ml of water after mixing thoroughly and mobile phase-B : Acetonitrile (100)% pumped
through column at a flow rate of 1.5ml/min. Buffer used in this method was 1.0 ml/min, ortho
phosphoric acid in 1000 ml of water.Temperature was maintained at 25°C. Optimized
wavelength for Lumacaftor was 222nm. Retention time of Lumacaftor was found to be 25
min, %RSD of system precision was found to be 0.8- 1.0. % RSD of method precision for
lumacftor was found to be 0.6 , and % recovery was obtained as 99.96%. LOD, LOQ values
obtained from regression equations were 0.02ppm, 0.07ppm respectively. Regression
equation of Lumacaftor was y = 16942.x + 543.0. Retention times were decreased so the
method developed was easy and economical that can be adopted in regular Quality control
test in Industries.

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OP-75: ISOLATION AND CHARACTERIZATION OF ACTIVE

NATURAL PRODUCTS FROM CORDIA DICHOTOMA
Gone. Swapna and Prof B. Kesava Rao *

*Department of Chemistry, University College of Sciences, Acharya Nagarjuna University,
Nagarjuna Nagar-522510, Guntur Dt, Andhra Pradesh, India.
krbhattiprolu@gmail.com2*
Drugs derived from naturally occurring herbal plants are serving to treat several
diseases to the mankind from several centuries. In this paper we are describing about the
isolation of active secondary metabolites from the plant Cordia dichotoma (Boraginaceae).
This plant was found to have several active compounds (1), and this plant is being used in
traditional medicine (2). The leaves of C.dichotoma reported to have anti-oxidant (3) and
contraceptive properties (4). We have isolated four compounds from this plant by using
sequential extraction, purified by column chromatography, crystallization and preparatory
HPLC. The spectrometric identification of these compounds is described in this present
paper. The isolated compounds are first time report from the bark of C.dichotoma.
OP-76: CONVENIENT PREPARATION AND DOCKING STUDIES OF

7-HYDROXYFLAVANONE BASED ACRYLATE COMPOUNDS AS
POTENTIAL ANTI-MICROBIAL AGENTS
P. Asha Bhanu, Prof. B.Kesava Rao*,

* Former Chairman-BOS, Department of Chemistry,
University College of Sciences, Acharya Nagarjuna University,
Nagarjunanagar-522510, Guntur Dt., Andhra Pradesh, India
bhanuphd.13@gmail.com, krbhattiprolu@gmail.com*.
Flavanones are important natural products having different pharmacological

properties such as antioxidant (1), anti-microbial (2), anti-inflammatory (3), antihypertensive
(4), antitumor (5) and antiviral (6). A series of flavanone acrylates (8a-k) were synthesized
starting from 7-hydroxy flavanone. All the compounds were evaluated for their anti-microbial
activity and in silico docking studies using proteins DNA gyrase B and sterol 14-α
demethylase. Compounds 8h were shown highest binding affinity scores in present series of
compounds. This is the first report assigning anti-microbial activity and structure activity
relationship of 7-hydroxyflavanone based phenylchromanyl acrylates.

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OP-77: SYNTHESIS AND IN-VIVO ANTI-INFLAMMATORY

ACTIVITY OF CINNAMYL ESTERS OF PHYTOSTEROLS
G. Usha Rani & B. Kesava Rao*

Department of Chemistry, Acharya Nagarjuna University,
Guntur, Andhra Pradesh
Plant sterols or phytosterols are naturally occurring molecules with steroid nucleus in
several plants and can regulate many biological processes. Phytosterols are structurally
similar to cholesterol with 28 or 29 carbon atoms but differ in carbon side chain with or
without a double bond and shows various health benefits especially cholesterol lowering
effects. The phytosterols are chemically modified at 3rd position to synthesize various
cinnamyl esters and their in-vivo anti-inflammatory activity was evaluated using
carrageenan-induced paw edema model. The resultant compounds have shown significant
inhibition of paw edema when compared to standard drug indomethacin and can be
developed as potent anti-inflammatory agents.
OP-78: SCHIFF’S BASE – ROLE IN BIOLOGICAL SCIENCES
Godha Lakshmi1; Hema Naga Chakra2; Prasanthi3, Basaveswara Rao4*

Department of Chemistry1,2,4, Krishna University Dr MRAR PGC, Nuzvid
Department of Biochemistry3, Krishna University Dr MRAR PGC, Nuzvid
EMAIL:godhanaidu@gmail.com
Schiff bases are common ligands in coordination chemistry. The imine nitrogen is
basic and exhibits pi-acceptor properties. The ligands are typically derived from alkSchiff
bases are active against a wide range of organisms since they play an important role in living
organisms, such as decarboxylation, transamination and C-C bond cleavage which are
important in the biosynthesis of hormones, neurotransmitters and pigments. Various industry
areas such as in food industry, dye industry, analytical chemistry, catalysis, fungicidal,
agrochemical and biological activities. In azomethine derivatives, the C=N linkage is
essential for biological activity, several azomethines were reported to possess remarkable
antibacterial, antifungal, anticancer and diuretic activities Barboiu et al (1996).Antibacterial
Activities Schiff base derived from indoline-2, 3-dione and 2- aminobenzoic acid and its Tin
complex showed antibacterial activity against Staphylococcus aureus. This activity might be
due to the presence of a hydroxyl and phenyl group Salvat et al., (2001). The increased
activity in the organo tin complexes may be due to the coordination and polarity of a tin (IV)
atom with oxygen of the ligand. Amino acid Schiff base derived from 2- hydroxy-5-

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methylacetophenone and glycine and its transition metal complexes showed bacterial
activities. The ligand was bacteriostatic against bacterial strains except Proteus vulgaris,
Shigella flexneri, and Bacillus coagulans. All complexes are either resistant or less sensitive
against P.vulgaris. Canpolat et al, (2004).The microbial activity of the N-(2-hydroxy-1-
naphthalidene) phenylglycine and its transition metal complexes has antifungal activity of
the ligand has ncreased upon complexation. Cu(II), Ni(II) Semicarbazones and
thiosemicarbazones complexes of Ni(II) metal showed antifungal activities against 11
pathogenic fungi. Sulekh Chandra, L.K.Gupta (2005). Antitumor and Cytotoxic Activities
Metal complexes of Schiff base derived from 2- thiophenecarboxaldehyde and 2-
aminobenzoic acid (HL) have been recommended and/ or established a new line for search to
new antitumor particularly. A tridentate Schiff basederived from the condensation of S-
benzyldithiocarbazate with salicyldehyde and its Zn, Sb, Cu complexes showed cytotoxic
properties Tarafder, (2000). Copper(II) complexes containing Schiff bases derived from S-
benzyldthiocarbazate and saccharinate showed anticancer properties. Antiviral Activities for
Schiff bases of gossypol show high antiviral activity. The main characteristic of an
antioxidant is its ability to trap free radicals. Research evidences suggests that antioxidants
reduce the risk for chronic diseases including cancer and heart disease. Literature shows that
the study of antioxidant property of Schiff base metal complexes (Demir, et al., 2015) has
been increased recently. To study the antioxidant potential through free radical scavenging by
the metal complexes, the change in optical density of DPPH radical were monitored. Schiff
bases have been receiving significant attention as one of the promising immobilization
alternatives for biopolymer biosensors and in biosciences.
OP-79: NUCLEAR CHEMISTRY – ROLE IN HUMAN LIVES
Saidathu, Sarveswara Rao, Basaveswara Rao*

Department of Chemistry, Krishna University Dr MRAR PGC, Nuzvid
Email: k.saidathu143@gmail.com
Nuclear chemistry is the sub-field of chemistry dealing with radio activity, nuclear
processes and transformations in the nuclei of atoms such as nuclear transmutation and
nuclear properties. It includes the study of the production and use of radioactive sources for a
range of processes. Nuclear chemists work with various isotopic forms of elements to study
fission and fusion processes, or they develop into the effects of ionizing radiation on
materials, living organisms (including people), and the environment. Nuclear medicine is a
specialized area of radiology that uses very small amounts of radioactive materials, or
radiopharmaceuticals, to examine organ function and structure. Nuclear medicine imaging is
a combination of many different disciplines. These include chemistry, physics, mathematics,

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computer technology, and medicine. This branch of radiology is often used to help diagnose
and treat abnormalities very early in the progression of a disease, such as thyroid cancer. A
tiny amount of a radioactive substance is used during the procedure to assist in the exam. The
radioactive substance, called a radionuclide (radiopharmaceutical or radioactive tracer), is
absorbed by body tissue. Several different types of radionuclides are available. These include
forms of the elements technetium, thallium, gallium, iodine, and xenon. The type of
radionuclide used will depend on the type of study and the body part being studied. The
different scans present to diagnose the diseased conditions of different body parts are Renal
scans, Thyroid scans, Bone scans, Gallium scans, and Heart scans, Brain scans, Breast
scans.They greatly improve the day to day quality of our lives. Nuclear methods in chemical
analysis: conventional and prompt gamma neutron activation analysis, gamma spectroscopy,
alpha and beta backscattering. Heavy ion radiation biology is an applied science involving
atomic, nuclear physics, biochemistry and molecular biology accelerates the charged particles
on the biological systems at the molecular level to study the modelling radiation effects,
Heavy ion cancer therapy, radiation protection in manned space flights, exploring different
pathways to radiation induced effects. Nuclear methods in structural research are Mössbauer-
spectroscopy, positron annihilation spectroscopy. Electron spectroscopies, Radiation
chemistry, Hot-atom chemistry, application of radioisotopes in industry. Stable isotopes in
nature, isotope enrichment methods. Nuclear reactors and energy production, Applications in
biochemistry, biology and medicine. radiation protection.
OP-80: MAGNETIC NANOPARTICLES – ROLE IN THERAUPETICS
Reyaz; Jayasri; Basaveswara Rao*

Department of Chemistry, Krishna University Dr MRAR PGC, Nuzvid
Email:skreyaz5556@gmail.com
Magnetic nanoparticles are a class of nanoparticle that can be manipulated

using magnetic fields. Such particles commonly consist of two components, a magnetic
material, often iron, nickel and cobalt, and a chemical component that has
functionality. Magnetic nanoparticles possess not only the general characteristics of
nanoparticles but also the magnetic properties. The concept of using magnetic micro- and
nanoparticles for drug delivery was proposed in the late 1970s by Widder, Senyi and
colleagues (Senyi et al 1978). Magnetic NPs own the following properties. Firstly, magnetic
nanometer materials have nonvirulence and nonimmunogenicity. Secondly, magnetic NPs
possess the surface effect. In detail, they have great specific surface area, which is good for
carrying a large amount of DNA fragments, drugs, and modified compounds. After
modification, they can be used as vector. Thirdly, excellent biocompatibility; fourthly,

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superparamagnetism. These particles may have magnetic cores with a polymer or metal
coating which can be functionalized, or may consist of porous polymers that contain
magnetic nanoparticles precipitated within the pores. By functionalizing the polymer or metal
coating it is possible to attach, for example, cytotoxic drugs for targeted chemotherapy or
therapeutic DNA to correct a genetic defect. Surface-modified compounds are available,
including polyethylene glycol (PEG), polyethylenimine (PEI), folic acid (FA), liposome,
noble metal, and inorganic materials. Surface modification can enhance the water solubility,
biocompatibility, and stability of NPs; they thus can be served as vectors for drug delivery,
gene transfer, MRI, and thermotherapy (Ting Guo, et al, 2018).The application of nanoprobe
in MRI is one of research hotspots in molecular imaging field. After NPs coupled with
ligands or antibodies enter the human body, they may be bound with the specific receptors or
antibodies of tumor cells and thus be phagocytosed by tumor cells or bind onto the tumor cell
surface, resulting in NPs aggregating in tumor tissues. Research on magnetic nanoparticles
as vectors for studying gene therapy as a treatment into the target gene of subject. It is
another novel treatment for tumor after traditional surgery, radiotherapy, and chemotherapy
(Fu et al., 2012). With the development of nanoscience and nanotechnology, the research and
application of magnetic NPs have made considerable progress in the biomedical field. For
example, magnetic NPs have been extensively applied in biomolecule vectors, targeted
localization, MRI, and thermotherapy. However, most studies remain at the laboratory
research stage, and these advances must be reachable to the mankind to combat the disease
progression.
OP-81: SYNTHESIS OF NOVEL DACLATASVIR ACID ANALOGUES

FOR NS5A HEPATITIS-C VIRUS INHIBITOR
Dr. Sd.Khasim Sharif*, Dr. Shaik. Mustafa

Department of Chemistry, Tagore Govt Arts & Science College, affiliated o Pondicherry
Central University, Pondicherry Pin: 605008.
Daclatasvir is hepatitis-c virus NS5A inhibitor. It stops HCV viral replication and
protein translation by directly inhibiting HCV protein NS5A. It was discovered by scientists
of Bristol- Myers Squibb. In the present study, we efficiently synthesized a series of novel
acid substituted Daclatasvir analogues as potential prodrug.The world health organisation
(WHO) 2014 guidelines for the screening, care and treatment persons with hepatitis C
infection state that worldwide more than 185 million people are infected with hepatitis C
virus (HCV). An estimated one third of these who become chronically infected (or)
hepatocelullar carcinoma. Nearly 350000 to 500000 die each year (HCV affected people).
Scheme:

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MDC O O
O
AlCl3 Cl Cl
+ Cl Cl
Reflux 12 Hours
Step-I
OH
ACN N
K2CO3 O
COB
Step-II
BOC
O
O O
N
O O
N
O
COB
950C-1000C Tolune
reflux CH3COO NH4
3 hours 2-Butanol
Step-III
BOC
H N N
N
N N
N H
BOC
CH3OH/HCl
Step-IV Reflux 2 hours
at 500C
H O
H N N
N
HO
N N +
H N H R
DIPEA EDC.HCl
1-HOBT.H2O
Step-V MDC
O
H N N
R N R
N N
N H
O
OP-82: A FACILE AND GREEN SYNTHESIS OF AG-GRAPHENE

NANOCOMPOSITE BY HYDROTHERMAL METHOD AND ITS
CATALYTIC REDUCTION OF 4-NITROPHENOL
Basavaiah Chandu,1 Syed Akhil,1 Syam Sundar Majety,1 Ganesh Majji,2 Vasu babu
Ala,3 Hari Babu Bollikolla.4
1 Dept. of Nanotechnology, ANU College of Sciences, ANU, Guntur, A. P. India -522510
2 Dept. of Chemistry, RGUKT-AP, Ongole Campus, Ongole, A. P. India -523001
3 Dept. of Chemistry, QIS College of Engineering & Technology, Ongole, A. P. India-523272
4 Dept. of Chemistry, ANU College of Sciences, ANU, Guntur, A. P. India -522510
Corresponding Author: chandupcu@gmail.com
In this work, a complete green synthesis approach to reduced graphene oxide-silver

(RGO-Ag) nanocomposite using Betel leaves extract as both reducing and stabilizing agent is
proposed. The precursor solution having GO and Ag+ ions was transferred to a Teflon coated
autoclave and heated at 160 0C for 12 h. Then, the reaction mixture was cooled and filtered to
get black precipitate of Ag-RGO nanocomposite. The Betel leaves extract (BLE) can
efficiently reduce GO and Ag+ ions to corresponding RGO-Ag nanocomposite. The

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composite was thoroughly characterized using UV-Visible, FT-IR, XRD, Raman and
HRTEM spectroscopic techniques. 4-nitrophenol (4-NP) in aqueous media is difficult to be
bio-degraded or self-cleaned, which causes a potential toxic threat to amphibians and aquatic
lives, even to human beings. So we envisaged the reduction of 4-NP with RGO-Ag
nanocomposite. In a typical catalytic reaction, aqueous solution of 4-NP and NaBH4 solution
was purged with nitrogen for 10 min to remove the dissolved oxygen. Then aqueous Ag-
RGO suspension was added to the reaction mixture and immediately transferred the contents
to a standard quartz cell and the UV-Visible absorption spectra were recorded with a time
interval of 120 s in scanning range of 200-700 nm at ambient temperature. The results
showed that the absorption peak of 4-NP at 400 nm was gradually reduced its intensity and a
new peak of 4-AP at 320 nm was gradually increased during the course of reduction reaction.
OP-83: Simultaneous Method Development and Validation of

Trastuzumab and Hyaluronidase-Oysk and Its Pharmacokinetic studies
with LC-MS/MS
Subba Rao Yarlagadda1, Pavani Y2, Subba Rao Mannam*

1, 2 Research Scholars, Department of Chemistry, Acharya Nagarjuna Univeristy, Guntur,
AP-522 510, India.
*Correspond Author: Department of Chemistry, Acharya Nagarjuna Univeristy, Guntur,
AP-522 510, India.
A highly reactive and simple LC-MS/MS assay was developed for the series of
changes in Trastuzumab and Hyaluronidase-Oysk using rat plasma. A mobile phase of 0.1%
Formic acid and Acetonitrile in the ratio of 70:30 was used and a positive mode of
electrospray ionization detection was carried out using MS. An isocratic mode using Waters
Symmetry C18 (150x4.6mm, 3.5μm) column was used. The method was validated with a
linear range 1.2-24ng/ml of Trastuzumab and 0.06-1.2ng/ml of Hyaluronidase-oysk. The

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%CV values of intraday, interday precision and accuracy were found to be within the
acceptance criteria. The %recovery of Trastuzumab was 99.5% and Hyaluronidase-oysk was
98.6% respectively. Using liquid liquid extraction, both the drugs were extracted from rat
plasma. From freeze thaw, auto sampler, bench top and long term stability studies, we found
that the drugs were stable throughout the stability studies. According to United States Food
and Drug administration guidelines, the Liquid Chromatography Tandem Mass Spectrometry
method was validated successfully. The pharmacokinetic research of Trastuzumab and
Hyaluronidase-oysk in rat plasma was utilized successfully using LCMS/MS.
Keywords: LCMS/MS, Trastuzumab, Hyaluronidase-oysk, Development, Validation.
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for modifiable lifestyle factors and breast cancer in New Zealand women. IMJ. 43
(11):1198-1204.
4. Yager JD, Davidson NE (2006). Estrogen carcinogenesis in breast cancer. N. Engl. J.
Med. 354 (3): 270‐82.

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OP-84: REMOVAL OF HEXAVALENT CHROMIUM IONS IN

SYNTHETIC WASTEWATER BY NOVEL BIOCARBON – A WAY
FORWARD FOR INDUSTRIAL WASTEWATER TREATMENT.
Singanan Malairajan1* Sundaram Rethinasabapathy2, Lingaraj Chellappa 1,

Sure Veera Venkatrao3 and Gunnam Nagarjuna4
PG and Research Department of Chemistry, Presidency College (Autonomous), Chennai – 600 005,
Tamil Nadu. Email: swethasinganan1966@gmail.com
Dept. of Chemistry, P.T. Lee C.N. Polytechnic College, Vepery, Chennai – 600 007, Tamil Nadu,
India.
Dept. of Chemistry, Rajiv Gandhi University of Knowledge Technologies, Nuzvid – 521 202, Andhra
Pradesh, India.
Dept. of Chemistry, SRR and CVR Govt. Degree College (Autonomous), Vijayawada – 520 004,
Presence of heavy metals in environmental systems may contribute adverse effects on

environmental and human health due to their acute and chronic exposure and magnifies
thorough food chain. Chemically, they are non – biodegradable in nature. It is also acts as
essential nutrients in human metabolism in limited quantities, but when threshold limits
exceeds, it causes severe problems to human beings. They are released into the aquatic
environment from various industrial activities. Heavy metals such as mercury, cadmium,
lead, chromium, nickel and copper are widely used in industrial productions and their
remains presents as toxic pollutants in the wastewater. Hence, treatment of wastewater and
possible removal of toxic pollutants are very essential before discharge into receiving
environment. There are number of methods and adsorbents were used in water treatment
process. Most of the conventional treatment methods of metal removal were often limited by
their operational cost and ineffectiveness at low concentrations. In this respect, a new search
is in progress to identify cheap materials which may have higher potential for the removal of
pollutants water and wastewater systems. In the current investigation, we introduced a new
biocarbon material, which are produced from a medicinal plant called Ocimum sanctum
(Lamiaceae) for the metal removal. This new biocarbon material is considered as a cheaper
and potential alternative adsorbent. In a model trial, 2.5g/100mL of biocarbon is used as
adsorbent to the removal of Cr (VI) ions with the initial concentration of 100 mg/L. At the
equilibrium time of 180min, at the working pH of 5.2, it was noticed that 98.45% removal of
Cr (VI) ions was observed. In real sample analysis, leather industry wastewater was tested
and treated with the biocarbon material. The analytical results found very much promising.
The removal process of metal ion on the biocarbon matrix is an ion-exchange mechanism and
also mainly depends on the physical characteristics of the materials.

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OP-85: APPROACHES TO DIGITIZE THE CHEMICAL INDUSTRIES

IN INDIA
Anupriya Koneru1 M.Sreelatha2 K.Lavanya3

1
Associate Professor, Department of IT,
2
Professor & Head, Department of CSE, RVR &JC, Guntur, India
3
Assistant Professor, Department of IT,
1,3
Lakireddy Bali Reddy College of Engineering, Mylavaram, India
In the present days the technology is growing rapidly. The technologies like Artificial
intelligence (AI), Machine learning (ML) and Internet of Things (IoT) bought the society
closer. These technologies have changed our life styles and automated many things. If
chemical industries would like to stay competitive in a changing world, they need to rapidly
transform their digital technologies with innovative technologies. This abstract discusses on
challenges faced by Chemical industries in India and providing solutions to these challenges
through the digitalization approaches. In our country, Chemical industries are facing
challenges like the instability of grist costs, currency rate of exchange, and charges undergoes
to make a dynamic situation in the chemical industry. If the manufacturer is not flexible that
definitely effects the business margins. Novelty is a key pole of competitiveness. The
requirements of the consumers are changing and the products need to change based on these
requirements. In the conventional model of marketing, chemical companies maintain straight
associations with their consumers. But now a days consumers are looking for the right
products and sellers online. Chemical industries that are not in close communication with
their consumers may miss the important information regarding the consumer profiling and,
ultimately, may lose these consumers. Companies have constantly used mergers and
acquisitions (M&A) to raise, develop their strategic direction and merge the divisions in
which they operate. These unifications increases the complexity of execution. In the process
of digitalization, the recent technologies like AI, ML and IoT could solve many chemical
industry problems. To provide the solution for instability of grist cost, use prediction
algorithms in the machine learning to predict [1] the grist cost and take the decisions based on
it. The most preferable algorithms for this application are Naive Bayes and Support Vector
Machines. To provide novel solutions for chemical industry, Intelligent and optimized
solutions [2] are available through AI & ML. To understand the needs of the consumers in
the chemical industry, use Data analytics. It offers better understanding of consumer
requirements. In the case of online trading, to improve the sales of chemical products choose
the proper portals which had already established the relation with the consumers or otherwise
decide which products need to sell through online portals and which products may sell
directly. To take these sort of decisions the ML techniques like Association rule mining helps
the Industries. The best algorithms for this process are Apriori algorithm and FP-growth

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algorithm. To reduce the complexity of execution in the chemical industry during M&A, use
IoT which automates many of the industrial procedures. This automation reduces the risks
and improves the performance.
OP-86: DEVELOPMENT AND VALIDATION OF THE HEAD SPACE

GAS CHROMATOGRAPHY FOR SIMULTANEOUS
QUANTIFICATION OF ORGANIC VOLATILE IMPURITIES IN
ENROFLOXACIN API AND ITS PHARMACEUTICAL DOSAGES
J. Krishna Kishore, K. PrasadaRao*

*Department of Chemistry, Bapatla Engineering College, Bapatla- 522101, Andhra Pradesh,
India.Email:krishna4kishorephd@gmail.com
A simple and rapid gas chromatographic method for quantification of organic volatile
impurities present in Enrofloxacin and its pharmaceutical dosage forms is described. The
organic solvents such as Methanol,1-Butanol, and Toluene are frequently used in the
manufacturing of Enrofloxacin. Even after such a manufacturing process, some solvents
(OVI’s) still remain in small quantities [1]. So in this work to quantifies that type of solvents
(OVI’s) as per ICH guidelines.Method for the quantification of residual solvents (OVI’s)
present in Enrofloxacin API was done by headspace gas chromatography with flame
ionization detector [2] and utilizes the Shimadzu GC- 2010 with FID capillary column,
Nitrogen as carrier gas with a flow rate of 3.0 mL/min. The critical experimental parameters
such as oven temperature, zero air, make-up flow, injection temperature; split ratio,
headspace conditions and the selection of diluent were studied and optimized.The retention
time of various residual solvents (OVI’s) taken individually and in spiked standard solutions
were determined. The retention times are 2.30 min for Methanol, 7.07 for 1-Butanol and 8.48
for Toluene respectively. The USP resolution is more than 5.0 for four solvents. The % RSD
for six injections should be NMT 10%. The recovery ranges from 85-115%. The correlation
coefficient (R2) is NLT 0.999. The LOQ and LOD were found to be 252 ppm, 83 ppm for
Methanol, 100 ppm, 33 ppm for 1-Butanol and 56 ppm, 19 ppm for Toluene. Precision,
method precision, intermediate precision and in dosage forms were found to be within the
acceptance limit[3]. From the obtained validation results the proposed method has been
successfully applied for the quantification of organic volatile impurities present in
Enrofloxacin API and its pharmaceutical dosage forms.

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OP-87: NOVEL BENZYL IMIDAZOLYLCARBAMATES SYNTHESIS

FROM Α-AMIDO SULPHONES
T. Vijaya Durga, K. Santha Kumari, B. Hari Babu*

Acharya Nagarjuna University, NNagar-522 510, AP-INDIA
Email: dr.b.haribabu@gmail.com
An efficient and convenient protocol was developed for the synthesis of novel benzyl
(1-methyl-1H-imidazol-5-yl) (aryl) methyl carbamate derivatives from α-amido sulphones
with a solution 5-bromo-1-methyl-(1H)-imidazole in THF under Hexamethylphosphoramide
(HMPA) and t-BuLi medium at -78 oC to room temperature. The reactions were monitored
with TLC for about 3-4 h. The yields obtained were also considerably good.
Keywords: α-amido sulphones, benzyl (imidazolyl) (aryl) methyl carbamate,
Hexamethylphosphoramide, t-BuLi, Imidazole, imidazolyl carbamate.
N
Br N N
R1
O S O 2 N Cbz
t-BuLi N R2
NH H
-78 oC to rt
Cbz 4h
R R5 R3
R4
4a-4j 5a-5j
4a = 5a: R1= H, R2 = H, R3 = OMe, R4 = H, R5 = H

4b = 5b: R1= H, R2 = OMe, R3 = H, R4 = OMe, R5 = H
4c = 5c: R1= H, R2 = H, R3 = H, R4 = C(CH3)3, R5 = H
4d= 5d: R1= H, R2 = H, R3 = H, R4 = Me, R5 = H
4e = 5e: R1= H, R2 = H, R3 = H, R4 = H, R5 = H

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OP-88: STUDY OF NON-COVALENT INTERACTIONS DUE TO

STERIC AND ELECTRONIC EFFECTS IN THE LIQUID MIXTURES
OF N-METHYL-2-PYRROLIDONE AND ALKANAMINES THROUGH
THERMODYNAMIC AND TRANSPORT STUDIES
D. Bala Karuna Kumar* a , N. Shareef a, Y. Subbareddy a, P. Sugandha Kumarb, and

G. Srinivasa Raoc
a
Department of Chemistry, Andhra Loyola College, Vijayawada, A.P., India-520 008
b
Department of Chemistry, Acharya Nagaruna University, Nagarjuna Nagar, A.P., India-
c
Department of Physics, Andhra Loyola College, Vijayawada, A.P., India-520 008
*
Corresponding author. e-mail: dbkarun@ gmail.com;Fax: 0866-2486084
The conventional interpretation and examination of the properties of binary liquid

mixtures by excess thermodynamic functions has turn out to be more practicable as it offers
rich and more exhaustive information with regard to neighbouring environment of molecules
in the liquid mixture [1,2]. In industry, there is a continuous need for dependable
thermodynamic-data of liquid mixtures. This data offers information about the nature and
degree of non-covalent interactions that may be used for the process development in industry
besides suggesting molecular models for unfolding the solution structure.The measured
viscosities, densities and ultrasonic speeds for pure liquids and for the binary liquid mixtures
of NMP (N-methyl-2-pyrrolidone) with Propyl amine (PA) or Butyl amine (BA) or Dipropyl
amine (DPA) are used to calculate deviations in adiabatic compressibilities, excess
intermolecular free lengths, excess Gibb’s energy of activation of viscous flow, excess
enthalpies and deviations in viscosity. The excess values are fitted to a Redlich–Kister type
polynomial to obtain their coefficients and the corresponding standard deviations. The excess
properties are observed to be positive/negative depending on the molecular interactions
between dissimilar molecules. To further examine the solute–solvent interactions partial
molar volumes at infinite dilution and excess partial molar volumes are calculated. Also, the
experimental viscosities are compared by using different models for theoretical viscosity that
include Katti-Chaudhri, Heric-Brewer, Grunberg-Nissan and McAllister for the systems
studied to verify the applicability of these theories.

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OP-89: NEW AVENUES FOR THE GREEN ECONOMY AND

SUSTAINABLE FUTURE OF SCIENCE AND TECHNOLOGY
Sk. N. Shareef a D. Bala Karuna Kumar a

a
Department of chemistry, Andhra Loyola College, Vijayawada
The compound business assumes a principal job in continuing the world economy and
supporting future advances and logical advances in new materials, less harmful items,
sustainable power source sources, ecological assurance, mechanical procedures with vitality
effectiveness and inexhaustible crude materials. Green Chemistry (GC) or Sustainable
Chemistry points, under more noteworthy cultural desires, for a supportable worldwide fate
of the planet Earth, for the structure of concoction items that wipe out the utilization of
unsafe substances for man and the earth. GC offers improved substance process financial
matters, accompanying with a diminished natural weight. GC can be applied to structure
naturally kind engineered conventions, to deliver life-sparing prescriptions, naturally
agreeable agrochemicals, new proteins for biocatalytic synthetic procedures, inventive
sustainable power sources, vitality effectiveness in concoction responses, and imaginative
materials while limiting ecological effect.
Coordinated advancement created by biotechnological techniques an incredible
exhibit of enzymatic impetuses that are amazingly effective to catalyze compound responses
of modern and fine synthetic concoctions. The ―bionic leafǁ strategy accomplished the
parting of water by daylight, the generation of hydrogen and with the assistance of
microscopic organisms the development of isopropanol as biofuel. Biocatalysts utilized an
enormous scale in the pharmaceutical amalgamation conveying ease and great intermediates
and medications. Biodegradable polymers and polymers from carbon dioxide have been
progressed by numerous concoction organizations. Natural photovoltaic sun-powered cells
have been created for low-vitality generation photovoltaic arrangements providινγ power at a
lower cost than first-and second-age sun oriented advances. The utilization of ―greenǁ
solvents and mechanical responses in water has been applied in the most recent decade to
various mechanical procedures. Vegetable oils have been utilized in various applications,
including oil-based paints. The supplanting of oil with biomass as crude material for fuel and
concoction generation is an intriguing alternative for the advancement of biorefinery
buildings. Green Chemistry visualized mechanical mediations for conventional cultivating
rehearses that will decrease ecological contamination what's more, expanded yields of
numerous harvests. There is concentrated research on sustainable power hotspots for practical
stockpiling advances (batteries). This survey contains and introduces chosen to inquire about
papers and ventures on imaginative green science and green building which are expecting a
maintainable future for science and innovation and imaginative substance items.

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OP-90: ASTROCHEMISTRY: OVERVIEW AND CHALLENGES
D.Prasanna , K.NagaRamyaKrishna and K. Padmalatha

Department of Physical chemistry, Vijaya institute of Pharmaceutical Sciences for Women,
Enikepadu, Vijayawada – 521108, India
Astrochemistry is the study of the abundance and reactions of molecules in the

Universe, and their interaction with radiation. Astrochemistry may be applied to both the
solar System and the interstellar medium. Interstellar medium presents a very hostile
environment to the formation and survival of complex molecules. It is crisscrossed by violet
shocks and pervaded by intense UV-X ray radiation which can easily break delicate
molecular bonds. Even the composition of the interstellar medium presents a problem to the
formation of complex molecules, since the gas is mostly hydrogen and helium.
Astrochemistry turns out to be a rich and complex field. It embraces many disciplines and
enhances them by the mutual interactions that it stimulates. Astrochemistry address deeply
significant issues arising in the formation of galaxies, stars, planets and ultimately perhaps
the origin of life.
OP-91: CORROSION CHARACTERIZATION OF ALUMINIUM 7075 -

TITANIUM NITRIDE COMPOSITES
P.V.Krupakara1* and M.Singanan2

1
Department of Chemistry, Cambridge Institute of Technology, North Campus,
Bangalore – 562 110, Karnataka.
2
Department of Chemistry, Presidency College (Autonomous), Chennai – 600 005, T. Nadu.
*
Email: drkrupakara@gmail.com
Metal matrix composites are in demand nowadays with respect to their demand in the
engineering applications like aeronautical, automobile and marine industries. Continuous
studies with respect to mechanical properties of composite materials. Where as detailed
corrosion studies of the metal matrix composites is less. Hence in this paper a detailed study
of corrosion properties of composite materials with various techniques in different
concentrated solutions of sodium chloride have been done. The matrix alloy selected is
Aluminium 7075 alloy which is commercially available. The reinforcement added is 50-80
micron size titanium nitride particulates. The composites containing 5, 10 and 15 weight
percentage of titanium nitride particulates are manufactured by liquid melt metallurgy
technique. Preheated but uncoated titanium nitride particulates are added in to the vortex
created by an impeller in the molten aluminium alloy. Then stirred well, degasified by adding

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hexachloroethane tablets. Then the composites were casted using pre heated cast iron moulds.
The castings are subjected to machining to get specimens as per standards to conduct static
weight loss corrosion test, polarization test and electrochemical impedance tests using
electrochemical work station are conducted. Salt spray tests using salt spray chamber was
also conducted.. In all the tests corrosion rates are calculated and the results are simulated
using computer. The results reveal that the corrosion rates of the composite materials
decreases with increase in reinforcement content. Hence the composite materials are more
proffered than the matrix alloy in various applications already mentioned above.
OP-92: COMPETITIVE ADSORPTION STUDIES OF BORON AND

THALLIUM (I) IONS ON TO LIGNOCELLULOSIC CARBON
Dr.Ch.S.R.G.Kalyani
Assistant Professor, Department of Chemistry (PG), SVRM College (Autonomous),
Nagaram, AP., India;E-mail chsrgk@gmail.com
The contamination of water sources with heavy metals such as Boron and Thallium
(I) ions [1-2], has generated special concern because these are frequently mixed in Industrial
waste waters, complicating their remediation. Because of this it is important to study the
simultaneous adsorption of Boron and Thallium (I) to evaluate the effect of the interactions
present when the two metals meet in the middle. This situation has led to the use of
lignocellulosic based carbon like Ocimum sanctum (Holy Basil) bio-mass received much less
attention as precursor for the preparation of activated carbon as adsorbent material. In this
context, the objective of this work was to evaluate the simultaneous adsorption of B and Tl
(I) on an adsorbents prepared from holy basil indicated as (OSC) chemical treatment with of
0.5 M HNO3 and thermal at 200°C. Simultaneous adsorption tests of B and Tl (I) were
performed using different combinations of metal cations in aqueous solutions to assess the
effect of different concentrations. Different process parameters, equilibrium conditions and
kinetics were analysed. Operation parameters studied were initial metal ion concentration,
solution pH, adsorbent dosage and temperature. Isotherm models to assess adsorption
equilibrium in studies reviewed are discussed. Adsorption was found between 74 and 88% of
both metals. An antagonistic effect was presented between B and Tl (I), which compete to
occupy the active sites on the surface of the adsorbent material. Similarly, it was determined
that there was a preferential adsorption of Boron on the material regardless of its initial
concentration, indicating a greater affinity between the adsorbent B and Tl (I). Finally, the
results show that the affinity of each metal ion onto the surface of the activated carbon is
influenced by binary mixture pH values. The antagonistic competitive effect was observed
for two metals, which is relevant to the size and electro negativity of the metal ions [3]. It was

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also concluded from the present study adsorption capacity of prepared carbon samples
towards Boron and Thallium is more in single element system than binary system [4], it is due
to competitive adsorption process. The adsorbent material evaluated is efficient in the
simultaneous removal of two heavy metals such as Boron and Tl (I), so it could be used as an
alternative for decontamination of water sources contaminated with these metals.
OP-93: NEW CYTOTOXIC ICETEXANE DITERPENES FROM THE

STEM BARK OF PREMNA TOMENTOSA
A. Hymavathi,A,B K. Rekha,A K. Suresh Babua And J. Madhusdana Rao.A

a
Division of Natural Product Chemistry, CSIR-IICT, Hyderabad-7
b
Sreyas Institute of Engineering and Technology, Bandlaguda, Hyderabad-68
The genus Premna (Verbenaceae) comprises a group of more than 200 different trees,
distributed in tropical and subtropical areas of the world. Premna tomentosa (Verbanaceae) is
a well known medicinal plant, is one of the important constituents among ten herb
formulations called ‘Dashmula’, a decotion of ten plants used in Indian traditional system of
medicine. All parts of P. tomentosa have been employed for the treatment of various
disorders. Its bark extract is claimed to have a lasting cure for hepatic disorders. Extracts
from P. tomentosa leaves are known to have diuretic, hepatoprotective, antioxidant, lipid-
lowering, immunomodulatory activities, and protective against acetaminophen-induced
mitochondrial dysfunction properties. Earlier studies on this species have resulted in the
isolation of various compounds, including flavonoids, triterpenoids, and steroids. As part of
our continuing efforts directed towards the discovery of the structurally interesting and
biologically active compounds from the Indian medicinal plants, an initial screening
procedure was conducted using the cancer cell lines. Bioactivity-guided phytochemical
analysis of hexane extract led to the isolation of three new icetexane type diterpenes (1–3)
(Fig. 1) and the three known compounds (4-6).

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OP-94: DESIGN, SYNTHESIS AND BIOLOGICAL EVALUATION OF

SUBSTITUTED STYRYL INCORPORATED QUINAZOLINE
DERIVATIVES AS ANTICANCER AGENTS
Pruthu Kala,DittakaviRamachandran*
Department of Chemistry, AcharyaNagarjuna University, Nagarjuna Nagar – 522 510,
Andhra Pradesh, India;*Corresponding author Email: dittakavirc@gmail.com
A novel series ofsubstituted aryl ethynyl incorporated quinazolines (11a-j) were

designed and synthesized, and their structures were characterized by 1HNMR, 13CNMR and
mass spectral analysis. Further, these compounds were screened for their preliminary
anticancer activity against human cancer cell lines like MCF-7 (human breast cancer), A549
(human lung cancer), DU-145 (human prostate cancer) and MDA MB-231 (human breast
cancer). Among all compounds, four compounds (11b, 11c, 11d and 11g) were showed more
potent activity than etoposide.
O O 5 O Cl
N O H NH2 N NH N N
SOCl2, DMF
N NH2 reflux, 10 h N N reflux, 5 h N 7N
NH2
4 6
MeCN,
K2CO3, reflux, 8h MeO OMe
8 OMe
OMe OMe
OMe OMe
HN OMe O H HN OMe
N N AcOH, reflux, 12h N N

R
N N N N
11a-j 10a-j 9
R 11a; R = H 10a; R = H
11b; R = 3,4,5-trimethoxy 10b; R = 3,4,5-trimethoxy
11c; R = 3,5-dimethoxy 10c; R = 3,5-dimethoxy
11d; R = 4-methoxy 10d; R = 4-methoxy
11e; R = 4-chloro 10e; R = 4-chloro
11f; R = 4-bromo 10f; R = 4-bromo
11g; R = 4-nitro 10g; R = 4-nitro
11h; R = 4-cyano 10h; R = 4-cyano
11i: R = 3,5-dinitro 10i: R = 3,5-dinitro
11j; R = 4-bromo-3,5-dimethoxy 10j; R = 4-bromo-3,5-dimethoxy
Keywords:Rigosertib, Gefitinib, Erlotinib, quinazoline, styryl and anticancer activity.

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OP-95: Luminescence features of erbium ions in ZnO-Ta2O5/Nb2O5/ZrO2-

B2O3 glass system
A. Siva Sesha Reddy* and N. Veeraiah

Department of Physics, Acharya Nagarjuna University,
Nagarjuna Nagar- 522 510, A.P., India
Email: shivaseshareddy@gmail.com
Zinc borate glasses doped with 2.0 mol% of Er2O3 and mixed with thermally resistant
transition metal oxides viz., Ta2O5, Nb2O5 and ZrO2 were synthesized. OA and PL spectra
and decay profiles of these glasses were recorded at ambient temperature. The spectra were
characterized using Judd–Ofelt theory and various radiative parameters viz., transition
probability, A, branching ratio, and the radiative lifetime, of principal emission
transitions of these glasses have been evaluated. The results indicated a significant
enhancement in the intensity of green emission (related to 4S3/2 4I15/2 transition) of Er3+ ions
due to co-doping of the glass system with Ta2O5, Nb2O5 and ZrO2. Quantitative analysis of
the results has pointed out that ZrO2 is the most favourable oxide among the three co-dopants
for achieving the highest quantum efficiency of green emission. Results were further
analysed based on the structural modifications taking place in the vicinity of Er3+ ions due to
co-doping with Ta2O5, Nb2O5 and ZrO2 and the reasons for such improvement have been
identified by means of IR and Raman spectroscopy studies. Additionally, the 4S3/2 energy
level of Er3+ ions (from which the green emission is originated) seemed to have been
additionally by the energy transfer from the excited state of ZnO and contributed to the
amplification of green emission.
OP-96: Influence of iron ions on physical and spectroscopic properties

of zinc aluminoborate glass system
A. Venkata Sekhar, Ch. Chandrakala, G. Naga Raju*

Department of Physics, Acharya Nagarjuna University,
Nagarjuna Nagar- 522 510, A.P., India
Email: gnaga9@gmail.com
Zinc alumino borate glasses doped with different concentrations of iron oxide were
prepared by melt quenching method and their spectroscopic studies were investigated. The
chemical composition of the glasses chosen for this study is: 20 ZnO–5 Al2O3–75 B2O3:
xFe2O3 (0  x  1.0 in mol %). The prepared glasses were characterized by XRD and SEM to
confirm the amorphous nature of the samples. Spectroscopic studies viz., optical absorption

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and IR studies indicated iron ions exist in Fe2+ and Fe3+ valence states with different
coordinations in glass matrix, the Fe3+ ions were found be in both tetrahedral and octahedral
positions whereas Fe2+ occupy the octahedral positions. In the optical absorption spectra the
intensity of the bands observed due to the transitions of Fe3+ ions is observed to increase at
the expense of the Fe2+ ions band viz., 5T2g  5Eg indicating gradual decrement in the
concentration of Fe2+ ions with increase of Fe2O3 content. In the IR spectra, the intensity of
the tetrahedral bands FeO4 and BO4 is observed to increase at the expense of the bands due to
BO3, FeO6 units with the increase in the concentration of Fe2O3. This result clearly suggests a
gradual increase in the tetrahedral occupancy of iron ions in these glasses that alternate with
BO4 units. The concentration of Fe2O3 is increased, the iron ions mostly exist in trivalent
state and occupy tetrahedral and octahedral substitutional positions and strengthen the glass
network and such glasses are more corrosion resistant and exhibit transparency over the
broad spectral region.
OP-97: Studies on spectroscopic properties of Sm3+ ions in sodium

antimonite glasses co-doped with manganese ions
B. Madhavi, P. Pavani Koteswari Devi and V. Ravi Kumar*

Department of Physics, Acharya Nagarjuna University, Nagarjuna Nagar- 522 510, A.P.,
India * Email: vrksurya@rediffmail.com
Na2O-Sb2O3:Sm2O3 glasses co-doped with different concentrations of manganese ions

were prepared by melt quenching technique. A systematic study on optical absorption and
luminescence spectral properties of titled glasses has been presented. The optical absorption
spectra of the samples exhibited absorption bands at about 405 nm and 524 nm attributed
to6A1(S) 4T2(G) (Td) and 6A1g(S) 4T1g(G) (Oh) transitions of divalent manganese ions. In
addition, the spectra have exhibited absorption bands due to the Sm3+ ions in UV–visible and
NIR region.The pattern of the absorption spectra for all the glasses remained the same;
however, the absorption strength under the given peak is found to vary with the increase of
MnO concentration. The emission spectra of all the glass samples recorded at room
temperature at lexc = 402 nm. As the concentration of MnO increased gradually from 0 to 1.0
mol%, the intensity of all the above bands is observed to grow. Moreover, in the region of
orange-red emission (due to 4G5/2 → 6H7/2 transition) of Sm3+ ions; the Mn2+ ions also give
rich emission in this region due to 4T1g(G)6A1g(S) (Oh) transition. As a result the intensity
of PL emission is observed to enhance to a large extent. This is attributed to energy transfer
from Mn2+ ions to Sm3+.

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OP-98: RECYCLIZATION OF GREEN HOUSE GASES INTO FUEL

AND HYDROGEN GAS USING CATALYST TO REDUCE GLOBAL
WARMING
Sapavat Madhavi
Lecturer in Chemistry, O/o Commissionerate of Collegiate Education, A.P.
The emission of greenhouse gases like carbon dioxide, methane, and nitrous oxide can
cause global warming effects include rising sea levels, regional changes in precipitation,
more frequent extreme weather events such as heat waves, expansion of deserts and ocean
acidification etc., The reports of Climate model projections summarises that, during the 21st
century, the global surface temperature is likely to rise a further 0.3 to 1.7 °C (0.5 to 3.1 °F)
in a moderate scenario, or as much as 2.6 to 4.8 °C (4.7 to 8.6 °F) in an extreme scenario,
depending on the rate of future greenhouse gas emissions and on climate feedback effects.
To overcome such problems, it is suggested that, there is a great need to recycle
greenhouse gases into useful fuel and hydrogen gas etc. This step will lead to a remarkable
effort for reverse global warming. For this process, the use of effective catalyst such as
Nickel, Magnesium and Molybdenum can convert large amounts of greenhouse gases like
carbon dioxide and methane. The catalyst, made from inexpensive and abundant nickel,
magnesium, and molybdenum, initiates and speeds up the rate of reaction that converts
carbon dioxide and methane into hydrogen gas. This conversion is called 'dry reforming',
where harmful gases, such as carbon dioxide, are processed to produce more useful chemicals
that could be refined for use in fuel, plastics, or even pharmaceuticals. Further the
experiments with the use of nickel-molybdenum nanoparticles under a reductive environment
in presence of a single crystalline magnesium oxide show greater results in capturing green
house gases and make the environment on earth become safe and healthy.
Key Words: Global warming; greenhouse gases; catalyst; nanoparticles. Global warming;
greenhouse gases; catalyst; nanoparticles.
Journal Reference:
1. Youngdong Song, Ercan Ozdemir, Sreerangappa Ramesh, Aldiar Adishev, Saravanan
Subramanian, Aadesh Harale, Mohammed Albuali, Bandar Abdullah Fadhel, Aqil
Jamal, Dohyun Moon, Sun Hee Choi, Cafer T. Yavuz. Dry reforming of methane by
stable Ni–Mo nanocatalysts on single-crystalline MgO. Science, 2020; 367 (6479): 777
DOI:10.1126/science.aav2412
2. The Korea Advanced Institute of Science and Technology (KAIST). "New catalyst
recycles greenhouse gases into fuel and hydrogen gas." ScienceDaily. ScienceDaily, 18
February 2020. <www.sciencedaily.com/releases/2020/02/200218104741.htm>.
3. PCC AR5 WG2 Technical Summary 2014, pp. 44–46;D'Odorico et al. 2013
4. IPCC AR5 WG1 Technical Summary 2013, p. 57.
(RACPABS - 2020)
OP-99: Development of Flavone-Cyanoacetamide Hybrids as Multitarget

Directed Ligand against Alzheimer’s Disease
A.G. Damu1*, K. Yelamanda Rao1, S. Jeelan Basha1, S. Rajagopal2

1
Department of Chemistry, School of Physical Sciences, Yogi Vemana University, Kadapa.
2
Department of Plant Sciences, School of life Sciences, University of Hyderabad, Hyderabad.
agdamu01@gmail.com
Alzheimer’s disease (AD) is an irreversible, progressive neurodegenerative brain

disorder that assaults the central nervous system1. Facing the complex etiology of AD, at
present researchers are focused on developing new multi-targetdirected ligands (MTLDs) to
fight back against this disease. In line with this modern paradigm of AD, a congeneric set of
compounds composed of flavone and cyanoacetamide moieties have been synthesized and
evaluated as multifunctional agents against AD2,3. Biological evaluation demonstrated that
compounds with fused iminopyrene ring exhibited excellent inhibitory potency and
selectivity to AChE, good anti-oxidant activity, strong modulation effects on self-induced Aβ
aggregation, low cytotoxicity, and neuroprotectivity in human neuroblastoma SK-N-SH cells.
Experimental Approach Flavone Derivative Computational Approach

Further, a molecular interaction study of active compounds with AChE using
fluorescence, circular dichroism, and molecular docking methods suggested that these
derivatives bind strongly to the peripheral anionic site of AChE. Overall, the multifunctional
profiles and strong AChE binding affinity highlight compounds with fused iminopyrene ring
as promising prototypes than acyclic cyanoacetamides for further pursuit to develop as
MTDLs against AD.
References:
1. Walsh, D. M.; Selkoe, D. J. Neuron. 2004, 44, 181–193.
2. Basha, S. J.; Mohan, P.; Yeggoni, D. P.; Babu, Z. R.; Kumar, P.B.; Rao, A. D.;
Subramanyam, R.; Damu, A. G. Mol. Pharmaceutics, 2018, 15, 2206–2223.
3. S. Jeelan Basha, Y. Daniel Pushparaju, K.Yelamanda Rao, P. Mohan, Z. Raveendra
Babu, K. Kasi Viswanath, D. Mark Manidhar, A. Dinakara Rao, S. Rajagopal, A. G.
Damu, Bioorganic Chemistry, 88 (2019), 102960.
(RACPABS - 2020)
OP-100: Metal free Multi-Component Approach: Preparation of Benzoyl

Derivatives
Bajivali Shaik1, Mohan Seelam,2 Prasad Rao Kammela2*

1
Dept.of Chemistry, Gudlavalleru Engineering College (Autonomous), Gudlavalleru-521 356
2
Dept.of Chemistry, Bapatla Engineering College (Autonomous), Bapatla-522 101
Cheap, readily available and non-toxic molecular Iodine catalyst is used for
developing this methodology. This methodology involves subsequent reactions of
Nucleophilic substitution/Addition/Removal of sulphur. This method is simple, general, and
efficient and it is demonstrated under mild reaction conditions. In addition, functional group
tolerance and reaction mechanism have also been studied.
Keywords:
Iodine catalyst • Desulfurization • Benzoyl Tetrazoles • Benzoyl Guanidines • One pot
reaction
O N N
N
N N
H H
tN
I 2, E 3 , 4 h 93%
N ,rt
O Na 3
O O S
I. KSCN, EtOAc
Cl 80 °C, 1 h II. NH3, rt, 3 h
NCS N NH2
H
I2 O NH2
, E
Intermediate I Intermediate III NH t3 N
3 ,rt, NH
4h N
H
89%
References:
1. (a) Balme, G.; Bossharth, E.; Monteiro, N. Eur. J. Org. Chem., 2003, 4101. Kim, J.G.;
Jang, D.O. Synlett, 2010, 2093. (b) Zeng, Li. Y.; Cai, C. J. Comb. Chem., 2010, 12, 35.
2. Rauf, M.K.; Din, I.; Badshah, A.; Gielen, M.; Ebihara, M.; Vos, D.D.; Ahmed, S. J.
Inorg. Biochem., 2009, 103:1135.
3. (a) Pinapati, S.; Mandapati, U.; Rudraraju, R. ChemistrySelect, 2017, 2:295. (b)
Ramana, T.; Punniyamurthy, T. Chem. Eur. J., 2012, 18:13279.
(RACPABS - 2020)
OP-101: Degradation of Pesticides by Ferrous doped TiO2 Nanoparticles
Y. Prashanthi1, T. Nageshwar Rao2, V. Sridhar1

1
Department of Chemistry, Mahatma Gandhi University, Nalgonda.
2
Changwon National University, South Korea
Degradation of Pyrazosulfuron-ethyl under direct sunlight using Fe doped TiO2

nanoparticles (FeTiO2) as catalyst. FeTiO2 nanoparticles are synthesized and characterized by
scanning electron microscopy (SEM) combined with energy Dispersive X-ray analysis
(EDX) and Fourier transform Infrared Spectroscopy (FT- IR). The experiment was conducted
by spiking in three different aqueous buffer solutions having pH 4.0, 7.0 and 9.0 to give the
uniform concentrations of T0 – Untreated Control, T1 – Pyrazosulfuron-ethyl 70% WG @ 1
mg/L of water and T2 – Pyrazosulfuron-ethyl 70% WG @ 2 mg/L of water. The spiked
samples were kept under sunlight. The sampling occasions were 0, 1st, 5th, 10th, 24th, 48th, 72
and 96th hour for acidic water (pH 4.0), neutral (pH 7.0) and basic water (pH 9.0). The
collected samples were quantified using a validated HPLC-UV method. The DT50 (Half
Life) of Pyrazosulfuron-ethyl was calculated by regression analysis from the dissipation data.
Key words: Fe doped TiO2, Pyrazosulfuron-ethyl, HPLC-UV, SEM, TEM and XRD.
OP-102: Ru(III) CATALYSED OXIDATION OF BENZILIC ACID BY

PERIODATE IN ALKALINE MEDIUM
*Dr.Neeraja .Valluru 1 ,Sailaja .Valluru 2 , Dr.V. Phani Kumar 3
1 &3 Dept . of chemistry ,SRR &CVR Government Degree College ( A) ,Vijayawada.
2 Dept. of Chemsitry P.BSiddartha College of Arts &Science (A) Vijayawada.
Periodate oxidation is a classic reaction in chemical kineticsused to split bonda
between vicinal un substituted carbons bearing hydroxyl or amino groups. Kinetics of
oxidation of benzilic acid has been investigated by periodate using Ru(III) as catalyst in t –
BuOH – H2O mixtures in alkaline medium. The kinetic orders are zero order in oxidant, first
order in substrate, first order in Ru(III) and first order in hydroxide ion. The reaction rate
increase with decrease in dielectric constant . A mechanism involving complexation of
benzilic acid with Ru(III) is proposed. The Ru(III) – benzilic acid complex in a rate
determining step gives benzophenone.
Key words : Periodate , Benzilic acid .t-Butyl alcohol , Ru(III) catalyst
References:
1. U.Mishra, K.Sharma and V.K.Sharma, J.Indian Chem.Soc., 63, (1986) 586.
2. N.Venkatasubramanian and R.Natarajan, Indian.J.Chem.13. (1995) 261.
3. K.Vidyanathan and N.Venkatasubramanian. Indian.J.Chem.11.(1973) 1146.
(RACPABS - 2020)
OP-103: A One-pot synthesis of thiazolo[3,2-a]pyrimidine derivatives, their cytotoxic

evaluation and molecular docking studies
P. Thriveni, a T. Sekhar,b and A.Venkateswarlu b

a )Assistant professor, b) Research Scholar, Department of Chemistry, Vikrama Simhapuri
University, Nellore-524320, India.
Email: ptriveni1@gmail.com
The synthesis of heterocyclic compounds has been an important area of study as these
molecules are used in various fields such as agriculture, medicine, pharmacy and contribute
to potent and selective drugs. Thiazolopyrimidines are an interesting group of heterocyclic
compounds which shows a variety of medicinal properties such as anticancer, antimicrobial,
anti-inflammatory, antioxidant, antiparkinsonian, antitumor and antiviral. An
environmentally benign, viable procedure and efficient one-pot method has been developed
for the synthesis of novel series of 2,4-diaryl-6,7,8,9-tetrahydro-4H-benzo[4,5]thiazolo[3,2-
a]pyrimidine hydrobromide, derivatives 3 by the α-bromination of Cyclohexanone 1 with N-
Bromosuccinamide (NBS) and followed by cyclization with 3,4-dihydropyrimidine-2(1H)-
thiones, 2 respectively, in the presence of p-toluenesulfonic acid (PTSA) in acetonitrile. The
significant features of this method are novel, simple, inexpensive experimental procedure,
short reaction time, and good yield. Some of the synthesized compounds were evaluated for
the cytotoxic activity against human lung adenocarcinoma cell line (A549), human breast
carcinoma cell line (MCF-7), Tested compounds 3(b-e) showed the excellent anticancer
activity against various cell lines. Particularly compounds 3c and 3e with IC50 values of 2.2 ±
0.6µM, and 5.6± 0.4 µM showed best cytotoxic effects against A549 and HeLa cancer cell
lines. Furthermore, Molecular docking study was performed for synthesized compounds 3(b-
e) against topoisomerase-II by using anti docking method. Docking results of the compound
3e exhibited highest docking score than the standard doxorubicin.
O S
i) NBS , PTSA, CH3CN, 35-40oC
S N N HBr
, 50oC Ar1 Ar2
ii) HN NH
1 3 (a-h)
Ar1 Ar2
2
(RACPABS - 2020)
Molecular Docking Compound 3e interaction with Topoisomerase – II

Keywords: cytotoxicity, molecular docking, NBS, PTSA, thiazolo [3,2-a] pyrimidines, one-
pot synthesis.
OP-104: Action against chemical traces in food
*Dr.Neeraja .Valluru 1 ,Sailaja .Valluru 2 , Dr.V. Phani Kumar 3 ,Dr.Sudhamasa .B 4

1 ,3&4 Dept . of chemistry ,SRR &CVR Government Degree College ( A) ,Vijayawada.
2 Dept. of Chemsitry P.BSiddartha College of Arts &Science (A) Vijayawada.
Scientific knowledge about chemical contamination of food has developed

considerably in recent years. The main groups of chemical contaminants are a) They are not
intentionally added to food like intentional adulterants. b) Contamination can happen in one
or more steps in food production and packaging process. c) Severe illness is resulting if
consumer ingest enough of them. In our study we identified most of the residues in food
chemicals are pesticides ,veterinary drugs, migrants from packaging materials ,food
processing contaminants .In our study we identified majority of the people belongs to all
economical groups in urban areas of Andhra Pradesh consuming Tea , rice ,curries ,Tiffin
carried in plastic carry bags or plastic tins . By the above process in long time resulting
majority people are reporting kidney failure and throat cancer .In our research we are
reporting some controlling measures to women against presence of chemical traces in food .
References
1. Food safety chemistry by Liangli Yu, Shuu wang, Buo Guo son.
2. Ingelfinger J.R, Melamine and the global implications of food contamination, New
England journal of medicine – 2008, 359 (26) 2745-2748.
(RACPABS - 2020)
OP-105: STUDIES ON REACTIONS OF 2-CHLOROQUINOLINE-3-

CARBOXALDEHY- DE WITH DIFFERENT NUCLEOPHILES
K. Shiva Kumara,b*, A. Naidua and P. K. Dubeya

a
Department of Chemistry, Jawaharlal Nehru Technological University Hyderabad
College of Engineering, Kukatpally, Hyderabad, India-500 085.
b
Department of Chemistry, Osmania University, Hyderabad-500 007, India
shivakumarkota@yahoo.co.in
Interest in quinoline containing structures stems from their widespread occurrence in

molecules that display a plethora of useful biological properties.1-2 Quinoline derivatives have
found commercial application in veterinary medicine as anthelmintic agents and in such
diverse human therapeutic areas such as anti bacterial, anti-viral, anti-cancer etc. Therefore, it
has been considered worthwhile to synthesise quinoline derivatives as potentially biologically
active molecules.2-4 Here we are presenting reaction of the 2-chloroquinoline-3-
carboxaldehyde with systematic nucleophilic displacement of chlorine in quinoline with O, N
and S nucleophiles.
References
1. S. Emami, E. Ghafouri, M.A. Faramarzi, N. Samadi, H. Irannejad, A. Foroumadi, Eur. J.
Med. Chem. 68, 185–190 (2013).
2. A. Barteselli, S. Parapini, N. Basilico, D. Mommoa, A. Sparatore, Bioorg. Med.
Chem. 22, 5757–5765 (2014)
3. S. Vandekerckhove, T. Desmet, H.G. Tran, C.d. Kock, P.J. Smith, K. Chibale, M.
D’hooghe, Bioorg. Med. Chem. Lett. 24, 1214–1217 (2014)
4. N.C. Desai, G.M. Kotadiya, A.R. Trivedi, Bioorg. Med. Chem. Lett. 24, 3126–3131
(2014)
(RACPABS - 2020)
OP-106: “Thermoacoustic studies in binary liquid mixture of 2-

methoxyaniline with ketones at various temperatures.”
E.Kalavathia,b#, A. Venkatesulu b,*, M. Gowrisankar c

a
Research scholar,Department of Physics,school of sciences, NEFTU, AP 791001, India
b
Postgraduate and research studies, department of Physics, Govt First Grade College,
Hosakote, Bangalore Rural, Karnataka 562114, India
c
Department of Chemistry, J.K.C. College, Acharya Nagarjuna University,
Guntur 52006, AP,India
e-mail Id is venkatesuluadavala@gmail.com; e- mail Id is ethirajulukalavathi@gmail.com
Binary mixtures of 2-methoxyaniline with aliphatic ketones have been studied

extensively from the view point of their acoustic and thermodynamic behaviour at different
temperatures. Experimental values of soundvelocities (u), density (ρ) are used to compute
derived parameters viz., isentropic compressibility (Ks), Intermolecular free length (Lf) and
acousticimpedance (Z). These properties ofisentropic compressibility Ks and freelength Lf
decrease with mole fraction for the entire range of temperature of investigation where as
acoustical impedance increases.These trend of decrease /increase reveal the existence of
specific type of molecular interactions in the binary mixtures.
(RACPABS - 2020)
 PHARMACEUTICAL
OP-1: PARADIGM SHIFT IN TREATMENT USING BIOLOGICS AND

SMALL MOLECULES
Dr.Anil S. Khile, Ph.D

Senior General Manager- R&D; Pharmaceutical Science & Technology; Eisai
Pharmaceuticals India Private Limited; Vizag, India.
In recent years, the novel segment of biotechnology has gained the momentum into
the pharmaceutical sector. Biotechnology products – or biologics – cover a wide range of
molecular classes. They range from proteins, like monoclonal antibodies, to peptide drugs up
to vaccines as well as gene and cell therapy products.The benefit of biological products is
their high effectiveness and site specific when compared to small molecule drugs. They
usually react highly specific against one target, which results in less undesirable side effects
and low toxicity which is beneficial for patients. A major challenge for the success of
biologics, especially peptide and protein drugs, has been their lack of oral absorption.
Recently, new delivery systems have been developed to overcome this challenge. Another
hurdle to the wider adoption of biologics has been the transition of candidates from academic
to industrial research and difficulties in large-scale production of biotechnological products
as well as affordability. Compared to the production of small molecules, biologics production
is highly complex and need technological advancement to fix the quality. For example,
protein therapeutics are produced in living cells, they require several post-translational
modifications to enhance their specificity and complex purification processes to ensure their
safety. However, parallel many small molecules are getting approval for wider treatments and
also they are acting as a very important payload in case of ADC.This paradigm shifts in
treatment certainly well accepted however many challenges need to overcome towards
patient benefits.
(RACPABS - 2020)
OP-2: ANTI CANCER STUDIES OF ALOE SINANA ROOT AND LEAF

EXTRACTS OF ETHIOPIA WITH REFERENCE TO LUNG
CARCINOMA
Akalu Terfa1, Anuradha. G1, J. SreeKanth Kumar2, Hailemicahel Tesso1 and

Young-Sik Jung3
1
Adama Science and Technology University, Adama, Oromia, Ethiopia.
+ 251911889977, jitusegni@gmail.com, anuradha.mythri@gmail.com,
2
Acharya Nagarjuna University, Guntur, A.P, India.
+91 77806 75835, sreekanth.mythri@gmail.com.
3
Cancer and Infectious Diseases Therapeutics Research Group, Korea Research Institute of
Chemical Technology, Sinseongno, Yuseong, Daejeon 305-600, South Korea.
The study was aimed to evaluate the anticancer activity of crude methanol (MeOH)
extracts and its organic solvent fractions including Chloroform (CH), ethylacetate (EA) and
n-butanol (Bu) of Aloe sinana root and leaf latex on human cancer cell lines: A549 (lung).
Sulforhodamine B (SRB) in vitro cyto toxicity assay was used to determine the percentage of
growth inhibition and IC50 values of root and leaf latex extracts of Aloe sinana. The results
were compared to standard anticancer drug Etoposide. Results demonstrated that extracts and
solvent fractions of the root and leaf latex of A. sinana exhibited dose dependent cyto toxicity
effect on human cancer cell lines. In this study, all organic solvent fractions of the root of A.
sinana were significantly active (IC50 ≤ 30µg/ml) against A549 cancer cell line, but Crude
MeOH and CH of the leaf latex of A. sinana were significantly active (IC50 ≤ 30µg/ml)
against A549 cancer cell line. The highest cyto toxicity for root and leaf latex was found in
the chloroform fraction. Extracts and solvent fractions of the root and leaf latex were less
potent as a cyto toxic agent in the all cancer cell lines when compared with reference standard
anticancer drug Etoposide. American National Cancer Institute (NCI, USA) recommended
that an IC50 ≤ 30 µg/ml is considered active and a promising for purification of a crude
extract. Likewise, the broad spectrum of extracts and solvent fractions of this plant should be
studied for active principles, and the mechanism of cell growth inhibition along with further
anticancer activity in vitro and in vivo. Therefore, purified compounds as well as their
derivatives could hold great promise towards good active leads for the development of a new
group of cancer chemotherapeutics agents.
(RACPABS - 2020)
150 120
Net growth as % of control

Net growth as % of
100 100
Me 80 Me
50
CH 60 CH
0
EA 40 EA
‐50 1 2 3 4 5 6
control
BU 20 BU
‐100 0
1 2 3 4 5 6
Concentration (µg/ml) Concentratio
Concentration (µg/ml)
Fig. 1 Dose response effects of A. sinana root Fig. 2 Dose response effects of A. sinana leaf latex
on the viability of A549 cancer cell lines. on the viability of A549 cancer cell lines.
OP-3: STANDARDIZATION OF TRIVRUT (OPERCULINA

TURPETHUM LINN) A MIRACULOUS MEDICINAL PLANT W.S.R
TO PHYTOCHEMICAL ANALYSIS AND PHARMACOLOGICAL
PROPERTIES.
Dr.M. Prasanna lakshmi. Professor and HOD ,Dravyaguna department

Dr.P.Sugunajyothy MD.Ayu P.G Scholar
Dr.K.Madhusudana rao MD. Assistant professor,
Dr.N.R.S.Govt.Ayurveda college AyuVijayawada-Andhrapradesh.
Ayurveda a science of life has a comprehensive management with natural remedies.

Home remedies have long been used to combat ailments as an alternative to traditional
medicine.One of such common used herb is Trivrut of Convolvulaceae. The present study is
on phytochemical and pharmacological profile of Trivrut roots, collected from south Indian
states of market samples, identified and taken for study and for standardization. Trivrut roots
found to be a potent source of bioactive compounds such as α- and β-turpethein, turpethinic
acids and salicylic acid etc1, which are useful in fever, oedema, ascites, anorexia,
constipation, hepatosplenomegaly, hemorrhoids, fistulas, constipation, gout, bronchitis,
ulcers, tumors, obesity, jaundice and skin disorders.Hence it is reported to possess
pharmacological properties like antibacterial, Antiviral2, anti-inflammatory, analgesic,
antiarthritic, ulcer protective, antidiabetic, and cytotoxic 3 properties.
(RACPABS - 2020)
OP-4:
4: POTENTIATION OF APOPTOSIS BY NOVEL IR ((Ⅲ)
COMPLEXES ON TRIPLE NEGATIVE BREAST CANCER CELLS
Rajeeva Lochana Panchangam1, Kaushik Chanda*, Venkatraman Manickam*

Department of Biosciences, School of Biosciences and Technology,
Vellore Institute of Technology, Vellore-632014,
Vellore 632014, Tamil Nadu, India.
Although chemotherapy with cisplatin remains the mainstay treatment option for
treating triple negative breast cancer (TNBC), the development of drug resistance and severe
side effects makes it unproductive in treating TNBC (Lee and Djamgoz 2018) 2018). To address
this problem there is an utter need for finding novel non-platinum
platinum metal complexes for filling
the void in TNBC treatment. In this study, we demonstrated the effect of series of novel
cyclo-metalated 2H-indazoles
indazoles Ir (Ⅲ)
( on the proliferation MDA-MB-231,231, a TNBC cell line.
The in vitro cell viability
ability studies showed all compounds exhibited potential cytotoxic activity
against these cell lines. Enhanced cytotoxicity has been observed in cyclo cyclo-metalated
complexes when compared to ligands alone. Among these complexes evaluated, Ir Ir-3 complex
was foundnd to be most effective and selected for further investigation. We have analysed the
effect of Ir-33 on TNBC cell proliferation, apoptosis induction, cell cycle inhibition and cell
migration. Ir-33 inhibited the proliferation of TNBC cells by increasing the levels of reactive
oxygen species and triggering mitochondrial mediated apoptotic cell death pathway.
Complex Ir-33 should be further investigated in vivo to establish it as an alternative strategy to
improve TNBC treatment.
Graphical abstract
Fig 1. Schematic representation of proposed mechanism of Ir-3 Ir 3 induced apoptosis in TNBC

cells. Treatment with Ir-33 for 24 h at an appropriate dose induced the generation of Reactive
oxygen species (ROS) which causes mitochondrial membrane depolarization (MMP),
upregulated pro-apoptotic
apoptotic (Bax & Bad) down regulated anti-apoptotic
anti apoptotic (Bcl
(Bcl-2) molecules
leads to mitochondrial mediated activation of Caspase 3 and finally leads to caspase
dependent apoptosis in TNBC cells.

522510, Guntur
Guntur, A.P. 111
(RACPABS - 2020)
OP-5: SYNTHESIS, CHARACTERISATION AND BIOLOGICAL

EVALUTION OF MULTI COMPONENT REACTION PRODUCTS OF
7-CHLORO-6-FLUORO 1,3-BENZOTHIAZOLE 2-ISOCYANIDE .
Dr.D.Ravi Shankar Reddy, Burra Mahesh Reddy

Pharamaceutical Chemistry, University College Of Pharmacetical Sciences,
Acharya Nagarjuna University
α- acyl amide carboamides are important templets for synthesis of heterocyclic

scaffolds. Scaffolds mimic peptide and protein hormone functions in human biological
system, from 7-chloro-6-fluoro-1,3-benzothiazole-2-isocyanide based multi component
products are might fulfill the synthesis of high potential scaffold templets synthesis
(electronegative atoms like F, Cl substituents increases receptor binding potency of
molecule). N-7-chloro-6-fluoro-1,3-benzothiazole-2-yl)-2-(N,1-diphenylformamido)-2-
phenylacetamide derivative having good features to synthesize scaffolds.
OP-6: EFFECTS OF AEGLE MARMELOS (L.) METHANOLIC LEAF

EXTRACTS ON BIOCHEMICAL PARAMETERS IN DIABETIC RATS.
Ravi Babu Birudu1, P.Padmavathi*1, M.Sathish Kumar2

Department of Zoology &Aquaculture, Acharya Nagarjuna University, Guntur,
Andhra Pradesh, India1.
Department of Pharmacology, Bapatla College of Pharmacy, Bapatla, Andhra Pradesh, India2
Background: Aegle marmelos (L.) Correa is a widely distributed plant in India as

well as in South Asia. For more than several centuries it is being widely used for its
medicinal properties. Objective: The objective of the study was to evaluate the biochemical
changes in alloxan induced diabetic rats treated with methanolic leaf extracts of Aegle
marmelos. Materials and Methods: 6 treatment groups (namely control, diseased, standard
(Glimperide), low dose (100mg/kg), medium dose (250mg/kg) and high dose (500mg/kg) of
methanolic leaf extracts were used in the study The biochemical effects were evaluated by the
determination of Albumin to Globulin Ratio (A/G Ratio), Albumin, Amylase, Bilirubin,
Blood Urea, Blood Urea Nitrogen, Calcium, Direct Bilirubin, Globulin, Glucose- 6-
Phosphate, HB1Ac, Homocystein, Indirect Bilirubin, Inorganic Phosphate, Lipase, Mean
Blood Glucose, Serum Uric Acid and Vitamin D3. Results: There were no significant
changes in A/G Ratio among the treatment groups when compared with the diseased and
control treatment groups. Low and medium dose treated animals showed significant change
(RACPABS - 2020)
in albumin, bilirubin, calcium, direct bilirubin, indirect bilirubin, globulin, glucose-6-

phosphate, homocystein, inorganic phosphate, lipase and Vitamin D3 levels when compared
with standard treatment group as well as diseased group. Whereas low dose treatment group
animals showed significant increase in amylase and mean blood glucose levels and
significant decrease in HbA1c levels than the diseased treatment groups. Conclusion:
Through the biochemical changes it is evident that the low and medium dose of methonolic
leaf extract of Aegle marmelos can be used the treatment of diabetes and its complications.
Key words: Aegle marmelos, Rutaceae, Bael, Alloxan, Diabetes.
OP-7: ELECTRO-ORGANIC SYNTHESIS OF LATE PHASE

PHARMACEUTICALS
Dr. Anuradha.G1, Dr. Fedlu Kedir Sabir1, Prof. Tesfaye Biftu1

1
Assistant Professor, Program Chair, Director for Institute of Pharmaceutical Sciences,
Adama Science and Technology University, Adama, Ethiopia.
+251901723466, anuradha.g@astu.edu.et, fedluked130@gmail.com, etbir1@gmail.com
Electro-Organic Synthesis is emerged as a safer and best alternative to conventional

methods in the derivation of the active molecules for pharmaceutical applications. The
present study is focused on the synthesis of compounds from selected commercial or late
phase pharmaceuticals using organo electrolysis method. The drugs with known chemical
formulations are transformed in to new species through electrochemical process by varying
the types of electrodes, solvents and electrolytes at different voltages determined by a Cyclic
Volta gram study. The synthesized compounds were characterized using various analytical
techniques like UV-Visible, FTIR, NMR and Mass spectroscopy. Investigation of anti-
bacterial antifungal and anti protozoan activity of synthesized compounds was carried and
compared with the reference drugs. The relevance of this study is to investigate the active
compounds from the selected drugs used in medicine. Identification of new leads would help
World Pharmaceutical industries for venturing into similar kind of research areas. In the
present day exigencies it would be expedient to identify different biological, environmental
friendly and cost effective methods. In future the main focus is to elucidate newer molecules
which are exactly responsible for activity and to find more applications in the area of
medicine.
(RACPABS - 2020)
OP-8: EVALUATION OF ANTIDIABETIC POTENTIAL OF

FENUGREEK SEED IN PREDIABETIC PATIENTS.
Genet Alem Geberemeskel1, Tewabech Zewde2 and Kidanu Estifanos3

1
Department of Medical Physiology, Institute of Bio-Medical Sciences, College of Health
Sciences, Mekelle University, Mekelle, Ethiopia.
2
Department of Medical Physiology School of Medicine, College of Health Sciences Addis
Ababa University, Addis Ababa, Ethiopia.
3
Ben Meskerem Hospital and Prime Ben Meskerem University College of Health Sciences,
Mekelle, Ethiopia.*Email: ggenet83@gmail.com / genet.alem@mu.edu.et
WHO estimates that over 170 million people worldwide are affected with Diabetes
mellitus and its complications, the number is estimated to be over 380 million by the
year2030. Fenugreek is one of the medicinal plants used traditionally to manage diabetes
mellitus. In this study was to investigate the effect of fenugreek seed powder solution for
antidiabetic potential on prediabetic patients. A total of (N = 114) prediabetic patients without
any significant diabetes complication were selected. They were grouped into two groups: the
treatment group (n =57) consumed 25 g fenugreek seed powder solution orally twice a day
for one month and the second group is the control (n =57). Blood sample was collected from
each participant by a medical technologist/technician before and after the study. Fasting
blood glucose level was analyzed by using Mindray BS 200E fully automated clinical
chemistry analyzer. Treatment group had significantly reduced fasting blood glucose level
23.3% (184.88±38.9 vs 141.0±31.1 (P< 0.001)) compared with the baseline fasting blood
glucose level, and the control group141.0 ±31.12 vs 179.2 ±21.5 (P <0.001). However,
fasting blood glucose level in control group was not significantly changed 181.46±16.41
vs179.22±21.45 (p = 0.25). The present study showed that administration of fenugreek seed
powder solution had pronounced effects in improving fasting blood glucose in prediabetic
patients with no adverse effects. Therefore, fenugreek seed may provide new alternatives for
the clinical management of diabetes.
(RACPABS - 2020)
OP-9: EVALUATION, CHARACTERIZATION OF FORCED

DEGRADATION PRODUCTS OF LEVETIRACETAM BY LC-MS/MS,
1H, 13 C & 2D NMR
Gollu Siva sankara Rao1, S. Venkat Rao2

1
Hetero drugs Pvt ltd, 22-110, IDA, Jeedimetla, and Hyderabad, India-50055
2
R&D center (A DSIR approved facility), SMS pharmaceuticals Ltd, Sy. No.189, 190,
Gagillapur (V), Hyderabad-500047, India
Email Id: gssr1978@gmail.com, svr.org@gmail.com
This research describes the isolation, predication of proposed chemical structures of

the degradation products (DP) formed during degradation study of Levetiracetam (LCM) in
its drug substance and as well as in drug product by high performance liquid
chromatography (HPLC) coupled with mass spectrometry (LC-MS/MS). The drug substance
was subjected to stress conditions including acid, base, thermal, oxidation, humidity and
photolytic degradations. Based on the fragmentation pattern, the possible chemical structures
of the unknown/known impurities were characterized with 1H, 13 C & 2D NMR techniques.
DP1
O FD
DP2
N
NH2
DP3
O
OP-10: QUALITATIVE ANALYSIS OF CHLOROFORM AND

ETHANOL EXTRACTS OF CITRUS MEDICA L. - PERICARP
Mahesh Kancherla1*, Mohammad Younis Dar1, Zaheer Ahmed N1

1
Drug Standardization Research Unit, Regional Research Institute of Unani Medicine (Under
CCRUM, Ministry of AYUSH, Govt. of India, New Delhi), Chennai, Tamilnadu, India-600
013.E-mail: cfraumaheshkancherla@gmail.com
Medicinal plants play a prominent role in pharmaceuticals industry in developing

alternative drugs to overcome the pitfalls possessed by the synthetic drugs. Citrus Medica L.
is well familiar as “Citron”. The peel possesses citro-flavonoids which are stomachic,
stimulant, exporant, and diuretic, and they are anti-inflammatory and antihistamine in nature,
thus used as a tonic (1). The bioactive compounds such as carotenoids (β-Carotene, β-
(RACPABS - 2020)
Cryptoxanthin, Lycopene, Lutin, and Xanthin), flavonoids

flavonoids (Neoeriocitrin (27.6 mg/100 mL),
Naringin (26.1mg/100 mL), Neohesperidin (22.1mg/100 mL) (2) and phenolics are the main
phytochemical present in fruit part.
Due to its medicinal demand, herein we have reported the qualitative analysis including
physicochemical properties, phytochemical analysis and development of HPTLC fingerprint
profiles for chloroform and ethanol extractives at λmax 254 and 366 nm wavelengths for the
first time.
OP-11:
11: BIOLOGICAL PERSPECTIVE OF 2,5-DIAMINO
2,5 DIAMINO
THIADIAZOLE ANALOGUES
ANALOGUES FOR ANTIMICROBIAL ACTIVITY –
CONFORMATION WITH DFT STUDIES
Ch. Ravi Shankar Kumar* M.Prasanti1 and Anjali Jha2
*
Faculty, Dept. of Physics, Institute of Science, GITAM University,Visakhapatnam
1
Scholar, Dept. of Physics, Institute of Science, GITAM University,Visakhapatnam
2
Faculty,Dept.of Chemistry,Instituteof Science, GITAM University,Visakhapatnam
Drug delivery, a different approach in treatment of diseases but major challenge still
persists between theraupeutic and toxic activity. A great deal of work is reported with gene
pairs, plasma treated solutions and metal/nano complexes, however geometric approach for
study is still conceited. The present article focus on conformational studies of reported green
synthesis of bis-derivatives
derivatives of 2,5-diamino
2,5 no thiadiazole derivatives with Isatin /Chalcone /
Acridone that provide insights of antimicrobial activity. The link between Isatin /Chalcone /
Acridone and 1,3,4 thiadiazole -2,5-diamine
diamine was implemented with electron density method
using Gaussian package 6-311++(d,p)
6 311++(d,p) basis set to explore reason contributing to
antimicrobial response in terms of related molecular and electronic properties. The complexes
of acridone are more active against antimicrobial activity which was also supported by DFT
studies. Conformational
ormational studies reveal a reduced energy gap with high kinetic stability and
electrophilicity index in the complex with delocalized electrophilic sites of 1,3,4 thiadiazole -
2,5 diamine and nucleophilic sites of reactants .

522510, Guntur
Guntur, A.P. 116
(RACPABS - 2020)
Fig.1 ESP contour of Acridone Complex
OP-12:
12: BILAYER TABLET TECHNOLOGY: A NOVEL APPROACH
S. Venkateswara Rao, R. Vyshnavi*, Padmalatha Kantamneni

Vijaya Institute of Pharmaceutical Sciences for Women, Vijayawada, Andhra Pradesh, India.
Bilayer tablet is new era for the successful development of controlled release
formulation along with various features to provide a way of successful drug delivery system.
Controlled release dosage forms have been extensively used to improve therapy with sseveral
important drugs. Use of bilayer tablet is a very different aspect for anti
anti-inflammatory and
analgesic. Bilayer tablet is suitable for sequential release of two drugs in combination,
separate two incompatible substances and also for sustained release tablet in which one Layer
is immediate release as initial dose and second layer is maintenance dose. Bilayer tablet is
improved beneficial technology to overcome the shortcoming of the single layered tablet.
This article provides an overview of the bilayer
bilayer tablet technology, highlighting the main
benefits of this type of oral dosage forms while providing a description of current challenges
and advances toward improving manufacturing practices and product quality.

522510, Guntur
Guntur, A.P. 117
(RACPABS - 2020)
OP-13: BIOANALYTICAL LC-MS/MS METHOD FOR DETERMINATION AND

COMPARISON OF SELEXIPAG ASSAY IN VARIOUS BIOLOGICAL MATERIALS
AND ITS APPLICATION TO PHARMACOKINETICS STUDIES IN RAT PLASMA
Namburi L A Amara babu1, Kalyani Koganti1, Babji Palakeeti2, Koduri S V Srinivas3,

and Koya Prabhakara Rao1*
1
New Generation Materials Lab (NGML), Department of Science and Humanities, Vignan’s
Foundation for Science Technology and Research University (VFSTR) (Deemed to be
University), Vadlamudi, Guntur-522 213, Andhra Pradesh, India.
2
Department of Chemistry, National Institute of Technology Warangal, Warangal-506004,
Telangana, India.
3
Syngene International LTD, Bommasandra, Bangalore-560099, Karnataka, India.
*Correspondence: Prof. Koya Prabhakara Rao, New Generation Materials Lab (NGML),
Department of Science and Humanities, Vignan’s Foundation for Science Technology and
Research University (VFSTR) (Deemed to be University), Vadlamudi, Guntur-522 213,
Andhra Pradesh, India. Email: kprao2005@gmail.com, drkpr_sh@vignanuniversity.org,
Phone: +91-967617858
A rapid, sensitive and selective bioanalytical method was developed and validated by
Liquid Chromatography - Mass spectrometry (LC-MS/MS) for determination and
comparison of Selexipag % assay in various biological materials. Methods: Selexipag was
extracted and compared its % assay after protein precipitation technique from various
biological materials such as rat plasma, rabbit plasma, human plasma and urine. Ambrisentan
was selected as internal standard. Selected analytical column Waters, X-Bridge C18 3.5µ (50
x 4.6 mm), mobile phase consists of Hexane sulfonic acid and Acetonitrile (80:20 v/v) at a
flow rate of 1.0 mL /min in isocratic mode and Selexipag was determined by the +ve mode of
electrospray ionization by using Mass detector. The method was developed to assess the
lower limit of detection (LLOD) (0.5 ng/mL), lower limit of quantification (LLOQ) (5
ng/mL) concentrations and Linearity range of 1 ng/mL to 20 ng/mL concentration with
regression correlation coefficient 0.999 were observed for Selexipag in Rat plasma. The test
samples at lower, medium and higher concentrations of Selexipag shows precision (% CV
was 0.8 to 1.11) and accuracy results (97.3 % to 100.6 %) for inter-day and intra-day analysis
at 0.5, 5, 10, 15 ng/mL concentrations of Selexipag. Appreciable recoveries for Selexipag
were observed when extracted in Rat plasma compared with other biological materials.
(RACPABS - 2020)
 BIOLOGICAL
OP-1:TRANSCRIPTOMIC ANALYSIS OF COPPER STIMULATED

INDIAN WHITE SHRIMP (PENAEUSINDICUS)
1
Polaki Suman, 2 T. Samuel David Raj, 2 P. R. Vani
1 Department of Biological Sciences, Rajiv Gandhi University of Knowledge Technologies,
Srikakulam, Andhra Pradesh-532402. Email: drpsuman.biology@rguktsklm.ac.in
2 Department of Zoology, Dr.VSK. Govt. Autonomous College, Visakhapatnam
Heavy metals such as copper is a trace element generally found in very low
concentrations which is essential for life and function of specific enzymes, a few are
beneficial while many are highly toxic. Copper, a common chemical contaminant in aquatic
environment, is known to be toxic to aquatic life at high concentrations. Biomarkers have
been used increasingly to investigate the adverse impacts of pollutants on different aspects
of the structure and function of an ecosystem. In this study, the transcriptome of Indian
white shrimp was characterized by a comparison between control and Copper-treated samples
using RNA-sequence approach. Totally, 88234826 bp and 13.24G clean reads were obtained.
The total length and number of unigenes were 68411206 bp and 100918, respectively. The
maximal and average length of unigenes was 24906 bp and 678 bp, respectively (N50, 798
bp). 7115 of these unigenes accounted for 7.05% of the total that were annotated in all
databases. After annotation of assembled unigenes, 35619 of them were assigned into 3
functional categories and 56 subcategories using Gene Ontology; 18580 of them were
assigned into 26 functional categories using Clusters of Orthologous Groups of proteins;
16864 of them were assigned into 5 major categories and 32 subclasses using KEGG. Finally,
1730 genes were differentially expressed (DGEs), 9 overexpressed pathways such as protein
digestion and absorption, neuroactive ligand-receptor interaction, pancreatic secretion,
tyrosine metabolism, amoebiasis, ECM-receptor interaction, riboflavin metabolism, amino
sugar and nucleotide sugar metabolism and AGE-RAGE signaling pathway in diabetic
complications were significantly enriched (q < 0.05), and one down-regulated pathway
(Staphylococcus aureus infection) was significantly enriched (q < 0.05). Overexpression of
genes in the pathways of protein digestion or absorption (PepT1/SLC15A and ATP1B) and
environment information processing (COL1AS, COL4A; LAMA3_5, LAMB3; FN1 and TN)
may imply the potentially positive toxicity resistance mechanisms.
(RACPABS - 2020)
OP- 2: ANTIMICROBIALPOTENCY OF SILVER NANOPARTICLES

OF EUPHORBIA MILIIDES MOUL. STEM EXTRACT
A.Ch. Pradyutha1, V. Umamaheswara Rao2

1
Department of Microbiology, R.B.V.R.R Womens College, Hyderabad, Telangana State.
2
Dept. of Botany and Microbiology, Acharya Nagarjuna University, Guntur District, A.P.
The development of green processes for the synthesis of nanoparticles is evolving into
an important branch of nanotechnology. In the present study,silver nanoparticles were green
synthesized from freshly prepared aqueous stem extract of Euphorbia miliiby adding aqueous
solution of silver nitrate (AgNO3) at room temperature(Kasithevar et al., 2017). The silver
nanoparticles formed were characterized using UV-Visible spectrophotometer,
Transmissionelectron microscope, Scanning electron Microscope and FTIR studies(Devraj et
al., 2013). The initial yellowish brown colour of AgNO3 was changed to reddish brown and
finally to colloidal brown solution with addition of stem extract after reaction period of 15
min. This colour change is due to the Surface Plasmon Resonance phenomenon. The UV-
Visible absorption peaks between 434.11nm clearly indicates the formation of silver
nanoparticles.The shape and size of the synthesized AgNps was examined by TEM and
SEM.TEM images revealed the poly disperse nature of the synthesized silver nanoparticles
with a size range of approximately 60-100nm which confirmed the nanometer size of the
particles. The shapeof the synthesized nanoparticles was mostly spherical with few as
ellipsoidal. SEM image provided further insight into the morphology and size details of the
silver nanoparticles. The FTIR analysis showed the functional groups involved in the AgNps
formation. The antimicrobial potential of AgNps synthesized from the stem extract of
E.miliiwas evaluated against both Gram positive and Gramnegativebacteria and fungi. AgNps
synthesized from the stem of E.milii exhibited significant antibacterial activity against both
Gram positive and Gram negative bacteria.Micrococcus luteus MTCC 106 exhibited highest
sensitivity (20.5mmzone of inhibition) to the stem AgNps than positive control
(streptomycin). Sclerotium hydrophilum MTCC 2157 showed highest susceptibility
(25.2mm ZOI) towards the synthesized AgNps than the other tested fungi.This promised
result of antimicrobial activity divulges the pharmacological application of silver
nanoparticles against the diseases caused by both Gram positive and Gram negative bacterial
strains as well as fungal strains.
(RACPABS - 2020)
OP-3: STUDIES ON ACUTE TOXICITY OF GLYPHOSATE (SL 41%)

IMPACT ON FRESHWATER FISH CIRRHINUS MRIGALA
A.Rajeswari, K. Sunita And K. Veeraiah

Department of Zoology & Aquaculture, Acharya Nagarjuna University, Nagarjuna Nagar,
Guntur, Andhra Pradesh, India
Glyphosate is a non-selective systemic herbicide that is applied directly to plant

foliage (Tomlin, 2006). It was discovered by Monsanto chemist John Franz in 1970 (US
patent no 3799758). In 1970s glyphosate (41% SL) was marketed under the trade name
“Roundup” by Monsanto. Glyphosate (41% SL) act as a plant growth regulator when used in
smaller quantities (RED Facts, 1993). The acute toxicity tests for 24, 48, 72 and 96 h to the
freshwater fish, Cirrhinus mrigala was exposed to the toxicant glycil (SL 41%) (Glyphosate),
an organophosphate herbicide under static and continuous flow-through methods. The LC50
values obtained were 3.757, 3.461, 3.15 and 3.043 mg/L respectively for 24, 48, 72 and 96 h
for static; 2.71, 2.383, 2.181 and 2.087 mg/L respectively for continuous flow-through
method. The fish exposed to chemical showed erratic swimming, loss of balance, surfacing
and convulsions in a dose dependent manner. Glyphosate was reported as moderately toxic to
freshwater fish, Cirrhinus mrigala and producing altered behavioral pattern in dose dependent
manner. These changes were in agreement with the earlier reports. The results obtained in all
were discussed with the available literature. Hence, the present study revealed that, the LC50
value gradually decreased with the increase of exposure period and mortality rate increased
with increase in concentration of herbicide.
OP-4:SYNTHESIS OF NANOPARTICLES USING PLANT EXTRACTS:

CHARACTERIZATION AND BIOLOGICAL APPLICATION
B. Pavani Bai, M. Sandhya Rani, G. Gowthami, Sudhakar Podha*

Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna nagar.
Corresponding author Email.id : sudhakarpodha@gmail.com
Nanotechnology has become one of the most promising technologies applied in all
areas of science. Nanoparticles (NPs) cause attraction of researchers because of their unique
properties, owing to their small size (1–100 nm), large surface-to-volume ratio and increased
reactivity. The synthesis of metal and semiconductor nanoparticles is an expanding research
area due to the potential applications in the development of novel technologies. Especially,
biologically synthesized nanomaterial has become an important branch of nanotechnology.
The present work, described the synthesis of silver, copper and zinc oxide nanoparticles
(RACPABS - 2020)
(NPs) using aqueous plant extract and its antimicrobial activities. The nanoparticles was
obtain characterized by UV–Vis spectroscopy, X-ray diffraction (XRD), Fourier transform
infrared spectroscopy (FTIR), Scanning electron microscope (SEM) analysis and Energy
dispersive X-ray analysis (EDAX). In this study we also investigated antimicrobial activity of
green synthesized NPs. Finally concluded the silver, copper and zinc oxide nanoparticles
exhibited an interesting antimicrobial activity with both Gram positive and Gram negative
bacteria. Synthesized nanoparticles will be evaluated for their biological activity like
anticancer, antioxidant, catalytic activity. a) Anticancer activity: The in vivo and in vitro
anticancer activity of green synthesized Ag nanoparticles will be evaluated. b) Antioxidant
activity: The antioxidant activity will be checked by DPPH radical scavenging assay. c)
Catalytic Activity: The catalytic ability of green synthesized AgNPs will be investigated
using the reduction reaction of Methylene blue by NaBH4as a model reaction.
OP-5: NATURAL PRODUCTS AND THEIR ROLE IN MODERN

MEDICINE
Ch. Anithasmruthi, Lalitha Vinnakota And Dr.V.Venkata Rathnamma

Department Of Zoology And Aquaculture
Acharya Nagarjuna University, Guntur-522510, A.P, India.
Email: anithachittakula@gmail.com
Natural products have high structural diversity and unique pharmacological or

biological activities due to the natural selection and evolutionary processes that have shaped
their utility over thousands of years. In fact, the structural diversity of natural products far
exceeds the capabilities of synthetic organic chemists within the laboratory. Thus, natural
products have been utilized in both traditional and modern medicines for treating diseases.
Currently, natural products are often used as starting points for drug discovery followed by
synthetic modifications to help reduce side effects and increase bioavailability. In fact,
natural products are the inspiration for approximately half of U.S. Food and Drug
Administration approved drugs. In addition to medicine, natural products and their
derivatives are commonly used as food additives in the form of species and herbs,
antibacterial agents, and antioxidants to protect food freshness and longevity. Natural organic
products find their way into almost every facet of our lives; form the clothes on our backs, to
plastic and rubber products, health and beauty products are often divided into two major
classes: Primary and Secondary metabolites. Primary metabolites are organic molecules that
have an intrinsic function that is essential to the survival of the organism that produces them.
Secondary metabolites in contrast are organic molecules that typically have an extrinsic
function that mainly affects other organisms outside of the producer. Secondary metabolites
(RACPABS - 2020)
are not essential to survival but do increase the competitiveness of the organism within its
environment. Natural products, especially within the field of organic chemistry, are often
defined as primary and secondary metabolites. A more restrictive definition limiting natural
products to secondary metabolites is commonly used within the fields of medicinal chemistry
and pharmacognacy.
OP-6: HISTOPATHOLOGICAL STUDIES ON PHOSALONE INDUCED

TOXICITY IN RESHWATER FISH CTENOPHARYNGODON IDELLA
(GRASS CARP)
Dr. K.Nirmala1 and Dr. V. Venkata Rathnamma2

1. Dr. K. Nirmala, Teaching Assistant, Dept. of Zoology, Dr. Abdul Haq Urdu University,
Kurnool -518002. E-Mail: nirmalakallagadda@gmail.com
2. Dr.V.Venkata Rathnamma, Associate Professor, Dept of Zoology, Acharya Nagarjuna
University, Guntur-522510. E-Mail: dhone_venkata@yahoo.co.in
The toxic impact of phosalone (35% EC) an organophosphate histopathological

changes in certain tissues such as gill, liver and kidney of freshwater fish Ctenopharyngodon
idella (Grass carp) exposed to sublethal and lethal concentrations. these fish were
acclimatized to the laboratory conditions at 28±20C for 15 days, and the fish were exposed to
sublethal concentrations (0.16 ppm) and lethal concentrations (1.6 ppm) of organophosphorus
pesticide Phosalone for 24 hrs lethal and sublethal 4 day and 8 days along with controls. At
the end of the exposure period, fish were randomly selected for histopathological
examination. The toxicant caused highly marked pathological changes in the gill than in liver
and kidney. They include progressive degeneration, epithelial hyperplasia, degeneration of
primary gill lamellae and secondary gill lamellae, fusion. Phosalone caused profound
pathological changes under chronic exposure in fish liver such as degeneration of cytoplasm
in hepatocytes, formation of vacuoles, necrosis and nuclear degeneration. Renal excretion is
one of the ways of eliminating the non-detoxified toxicant molecule resulting in severe
pathological changes in haemopoietic tissue, severe necrosis; cloudy swelling of renal
tubules, disintegration of interstitial tissue, pyknotic nuclei, etc were also noticed in kidney.
Concentration and time dependent oxidative stress was observed in the tissues (gill, liver and
kidney) of C.idella at subletha and lethal concentrations of toxicant. All these changes
indicate the hepatotoxic nature of the Phosalone pesticide.
(RACPABS - 2020)
OP-7: PHYTOCHEMICAL SYNTHESIS OF SILVER NANOPARTICLE

SYNTHESIS USING H. TILIACEUSAQUEOUS LEAF EXTRACT AND
PRELIMINARY EXAMINATION OF ANTIOXIDANT AND
ANTICANCER PROPERTIES
Dr. P. Sudhakar, Asst.Prof. Department of Biotechnology, Acharya Nagarjuna

University.Guntur.A.P.522510. email in – Sudhakarpodha@gmail.com
K. Vinay Viswanath. Research scholar, Department of Biotechnology, Acharya Nagarjuna
University.Guntur.A.P.522510. email.id – kvv3686@gmail.com
The present study reports the synthesis and characterization of silver nanoparticles
using aqueous extract of Hibiscus tiliaceus L. leaves and its application in anti-oxidant and
anti-cancer studies. The phytochemical analysis has proved the presence of flavonoids which
played a major role in reduction and stabilization of silver nanoparticles. FTIR analysis
showed the other functional groups which played a role in reduction and stabilization of
AgNPs, X-ray crystallography (XRD) indicated that the AgNPs were in crystal form, UV–
VIS spectroscopy, DLS and zeta potential analysis proved the formation, average size in
colloidal form and stability of the AgNPs synthesized. FE-SEM, and HR-TEM showed the
size and shape of the nanoparticles which are spherical in shape and are of average size of 30-
35nm. EDAX confirmed the presence of silver. The synthesized AgNPs were applied for
antioxidant studies i.e., total antioxidant assay, DPPH assay, reducing power assay and metal
ion chelating assay which showed very good activity which were almost equal to standard
controls. The synthesized AgNPs showed good anticancer activity on MCF-7 cells with 88%
inhibition in MTT assay, which provides favourable methodology for alternate nano-drug
development.
OP-8: DETERMINATION OF BT GENE AND BT PROTEIN IN BT

COTTON SEEDS BY USING ELISA AND PCR SCREENING AND GEL
ELECTROPHORESIS.
Dr.K.Suneetha, K.Praveen Kumar,SK.Neha Fatima and SK.Razeena.

Department of Biochemistry, Acharya nagarjuna university.
Bt cotton contains genes from Bacillus thuringiensis that make the plant resistant to
the cotton bollworm complex. This inbuilt insect resistance can lead to savings in chemical
pest control and higher effective yields in farmers’ fields(1).Number of researchers have
shown that Bt cotton adoption is associated with significant benefits to farmers in various
(RACPABS - 2020)
countries (2). In addition to productivity gains (3), Bt adoption entails reduced incidence of
acute pesticide poisoning among small holders (4). During 2006–2008, the cost difference
was lower because of government interventions in seed pricing and increasing competition in
the market for Bt technology (5). This paper aim is to determine the Bt gene and Bt protein
in Bt cotton seeds which are obtaining from Bt cotton hybrids. In this ELISA test was
performed on the Bt hybrids to identify the Bt protein positive and negative seeds, then
isolated the Bt positive seeds. The isolated DNA solution was amplified through PCR
amplification method by using Bt primers. Finally the zygosity of the Bt gene in the isolated
DNA is tested by GEL Electrophoresis method.
OP-9: EVALUATION OF THERMAL AND MECHANICAL

CHARACTERISTICS OF PULLULAN EDIBLE FILMS
INCORPORATED WITH POLYSACCHARIDES AND PLASTICIZERS
G. V. S. Ramakrishna1,*, P. Sudhakar2
1
Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram,
A.P., India.
2
Department of Biotechnology, Acharya Nagarjuna University, Guntur, A.P., India.
*
Corresponding author: krishna.ganduri@kluniversity.in
Thermo-mechanical-physical characteristics of aqueous suspension-casted edible

films composed of 10 %(w/v) pullulan (10Pu), 10 %(w/v) pullulan + 0.5 %(v/v) gelatin
(10Pu_0.5G), 10 %(w/v) pullulan + 0.5 %(v/v) polyethylene glycol (10Pu_0.5PG), 10
%(w/v) pullulan + 0.5 %(w/v) sodium alginate (10Pu_0.5SA), 10 %(w/v) pullulan + 1
%(w/v) calcium chloride + 2 %(v/v) lime juice (10Pu_1CC_2L) were investigated by
Modulated differential scanning calorimetry (MDSC) analysis, Thermo-Gravimetric Analysis
(TGA), Universal Testing Machine (UTM) analysis, Differential scanning calorimetry (DSC)
analysis, Scanning Electron Microscopy (SEM) analysis, X-ray diffraction (XRD) analysis
and Atomic force microscopy (AFM) analysis. Glass transition temperature was increased
due to addition of 0.5 %(w/v) polysaccharides and plasticizer, when compared to pullulan
alone films (Ebrahimi et al., 2016). The X-ray diffractograms shows 10Pu_0.5G film presents
slightly longer peak height than remaining films implies a tendency toward high crystalline
structure. All the films showed very comparable tensile properties, although the elongation at
break of 10Pu films was higher, while other films displayed the lowest elastic modulus and
tensile strength, and low elongation at break among the studied samples (Ghasemlou et al.
2011b). SEM analysis shows that the outer surfaces of all the film samples were uniform and
the appearance of films incorporated with plasticizers (polyethylene glycol, calcium chloride)
were generally smooth and homogeneous (Zhang and Shi, 2016). 2-dimensional (2-D) and 3-
(RACPABS - 2020)
dimensional (3-D) AFM images indicated the morphology (qualitative parameter) and the
surface’s roughness (quantitative parameters) of different pullulan-based films shown that
pullulan bonding between gelatin, sodium alginate, plasticizers molecules and water
molecule, van der Waals forces, and electrostatic interactions between polysaccharides,
plasticizers were also observed (Shi et al., 2012).
Key words: Pullulan, films, thermal, mechanical, characteristics, morphology.
References:
 Ebrahimi et al. (2016). Food hydrocolloids, 54, 302-314.
 Ghasemlou et al. (2011). Carbohydrate Polymers, 84(1), 477-483.
 Zhang and Shi (2016). Carbohydrate polymers, 153, 345-355.
 Shi et al. (2012). Journal of agricultural and food chemistry, 60(23), 5988-5993.
OP-10: ROLE OF BIO INFORMATICS IN ANIMALS AND DRUGS
G.Mounika, A.Anitha and Dr.V.Venkata Rathnamma

Department of zoology and Aquaculture
Acharya nagarjuna University,Guntur-522510,A.P,India
Email:mounika geedigunta@ gmail .com
The bioinformatics involves compailation and storage of biological data across the
globe inorderly and deciplined manner and also include the analysis interpretation and
various biological data including nucleotide sequences,aminoacid sequences, protein domains
and protein structures It also involves development of new algarithams and statistics to assess
the biological information such as relationship among the members of large data search.It
also helps in making sense of biological data in away to improve the understanding of
organisms,plants,animals,and human being.Which intern has implication and heip to
medicine and it heips to integrate the data in a manner inableling cross linking current
biological research out come with that which was generated in the past.Though ever sense
there has been emergence and sequences from the field of genomics there has been past
amount of data pertaing to DNA sequencing including the human genomic project but side by
side there has been an emergency of transcriptomics which pertain to studing the expression
levels of defferent messenger RNA from particular organism (or) tissue as particular point of
time,this had simultaneously validated &substantiated the field of genomics.However
because of alternative splicing and because RNA is highly degradable by RNA ases there
emerse the field of proteomics which pertanines to studing of proteins and detailed analysis
of their expression,structure,functionand interactions.These together from the different fields
constistute the biological data.
(RACPABS - 2020)
CHEMO INFORMATICS:The use of information technology and management has become a

critical part of drug discovery process.Possibly the most defining features of a drug is its
ability to interact with its biological target.Today drug discovery is a highly rigorous process
that spns across structural and cell biology.Bio informatics and computational and medicinal
chemistry
Key words:Nucleotides,Genomics,Transcriptomics,Proteomics.
OP-11: ANTIBACTERIAL ACTIVITY OF PSEUDOMONAS

AERUGINOSAVUR-Z ISOLATED FROM POLLUTED WATER OF
RIVER MUSI AND LAKE HUSSAIN SAGAR
Konatham Samuel John and Umamaheswara Rao V*

Department of Botany and Microbiology
Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh - 522510
Metabolic pathways play an important role in living organisms. They are very
important in breaking and making of bonds to form various metabolites, which are of
immense value to the living organisms. Secondary metabolites are one of them,which also
include antibacterial metabolites that are produced by one microorganismwhich inhibit the
growth of other microorganism. Today, antibacterial metabolites are like chemical weapons
in the hands of humans to combat a number of infectious diseases. Bacteria are the best
choice for exploitation, among all other microorganisms, because of their numerous abilities
in varied conditions. It is well known that highly polluted water exerts both biotic and abiotic
stress on microbes (Tripathyet al., 2009), which compels the bacteriato undergo various
modifications in their metabolism for their survival in that habitat.So, the present work has
focussed onscreening the bacteria isolated from polluted waters of River Musi and Lake
Hussain Sagar for antibacterial metabolite production.Bacteria were isolated from the
collected polluted water samples by plating method. The representative colonies of the
isolates were subcultured and then checked for their antibacterial activity against some
selected Gram positive and Gram negative bacteriaby Agar well diffusion method
(Mohammad et al., 2011). One of the isolates, which was identified as Pseudomonas
aeruginosa by 16S rRNA sequencing and named as Pseudomonas aeruginosa vur-z, wasfound
to be a potent producer of antibacterial metabolite.The isolate has shown antibacterial activity
against 12bacteria, of the 16 test bacteria used in the study. Maximum antibacterial activity
was noted against Salmonella entericaand minimum zone of inhibition was noted against
Alcaligenes faecalis and Streptococcus mutans. Further work on isolation, characterization
and identification of the potent antimicrobial compound from this strain is under progress.
(RACPABS - 2020)
OP-12: STUDIES ON EFFICACY OF ENCAPSULATED POTENTIAL

PROBIOTICS BEADS AGAINST THE VIBRIOSIS IN SHRIMPS
THROUGH ORAL ADMINISTRATION
K. Rajyalakshmi, Syed Shabana, M.Kishore babu, Dr. Alapati Krishna Satya*

Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar,
Guntur, Andhra Pradesh -522510, India.
Corresponding author e-mail: akrishnasatya78@gmail.com.
Probiotics have recently captivated extensive attention in aquaculture which is an

alternate method to antibiotics in aquatic disease management. The trend of using probiotic
micro organisms in aquaculture is increased due to research results indicating their ability to
increase production and prevent diseases in aqua farming. Bacterial encapsulation has been
used to maintain probiotic viability in human food; however, the usefulness of the
encapsulation techniques in animal and aquatic feed needs to be evaluated. Our present
investigation was carried out to evaluate the probiotic potentiality of shrimp gut isolates on
pathogenic Vibrio parahaemolyticus, Vibrio cholera, Vibrio harveyi and Vibrio alginolyticus
in shrimps. Initially probiotic products were isolated from healthy shrimp gut and isolation
process was carried out by using appropriate media MRS agar. Thirty five probiotic strains
were identified based on morphology and biochemical characterization. Out of these 25
potential strains were selected by performing pH (7.0 -14.0) NaCl (3-9%) and temperature
(300C, 350C, 370C, 400C, 450C) tolerance assay. Further these promising strains were
analyzed for antimicrobial against the vibriosis causing agents and pathological activity.
Isolates were Lactobacillus acidophilus, Pediococcus acidilactici, and Lactobacillus
rhamnosus are identified on molecular characterization. Encapsulation work is executed for
promising probiotic organisms along with the storage analysis. The promising potent
encapsulated and un-encapsulated probiotic bacteria were analyzed in 50Lt tanks in matrix at
different stages of shrimp farming (juvenile and adult). The encapsulated disease resistant
probiotic products will be very helpful in eradicating vibriosis in shrimps.
(RACPABS - 2020)
OP-13: ENVIRONMENTAL POLLUTION AND PROTECTION
K. Saicharitha, Sudharani Chalapala and S. Anwar

Silver Jubilee Government College, Kurnool-515002.
Environmental Pollution is defined as “the contamination” of the physical and

biological components of the Earth/Atmosphere system to such an extent that common
environmental processes are adversely affected. “Pollutants” can be naturally occurring
substances or energies, but they considered as contaminants when in excess of standard
levels. Any use of natural resources at a rate higher than nature’s capacity to restore itself can
result in pollution of air, water, and land.
Steps to be taken for the Protection of Environment:
1. Increasing efficiency in energy use.
2. Use of environmentally benign fuel sources.
3. Reducing the use of water and chemical inputs in agricultural sector.
4. Adorption of less environmentally harmful pesticides or cultivation of crop strains with
natural resistance to pests in agriculture.Pollution prevention reduces both financial costs
(waste management & cleanup) and Environmental costs (health problems and
environmental damage). Pollution prevention protects the Environment by conserving
and protecting natural resources which strengthening economic growth through more
efficient production in industry and less need for households, businesses and
communities to handle waste.
OP-14: ANTIPLASMODIAL ACTIVITY OF CLEOME VISCOSA

AGAINST CHLOROQUINE SENSITIVE PLASMODIUM
FALCIPARUM (3D7 STRAIN) AND CHLOROQUINE SENSITIVE
PLASMODIUM BERGHEI (ANKA)
K. Sunita, G. Sravani, D. Santha Kumari and Amna Ali Alhadad

Department of Zoology and Aquaculture, Acharya Nagarjuna University,
Nagarjuna Nagar - 522510, Guntur, Andhra Pradesh, India.
Malaria is a deadly parasitic disease, every year ten million cases are being diagnosed
mainly from Africa and Southeast Asia and approximately 2 million people died with malaria
(World Malaria Report, 2014). The spreading and development of resistance is a great
problem to malaria treatment and control. So there are critical challenges that still deserve
attention in order to boost malaria prevention and control because of the resistance of
parasites and recently developed RTS,S vaccine does not protected Plasmodium vivax
(RACPABS - 2020)
malaria and partial protection against P. falciparum (Benelli and Mehlhorn, 2016). Hence, the
plant based formulations are an alternative source for development of new drugs for
treatment of malaria. Thus the present study was aimed to evaluate the antiplasmodial activity
of the plant Cleome viscose (Devi et al., 2002).
The collected plant parts were washed with tap water and dried in shade under
laboratory condition. The crude extracts were extracted through soxhlet apparatus, the
residues were concentrated in rotary vacuum evaporator. The parasites were cultured in
RPMI medium supplemented with O+ serum in 96-well plate. The THP-1 cell lines were also
cultured in RPMI medium in 96-well plate. In vivo studies were conducted in BALB/c albino
mice.Of all the extracts the ethyl acetate extract of leaves has shown excellent antimalarial
activity with IC50 value of 7.00 µg/ml. In cytotoxicity evaluation of crude extracts, none of
the extracts has shown cytotoxicity on THP-1 cell line. The in vivo study has revealed that
the ethyl acetate leaf extract of C. viscosa displayed a very good activity against Plasmodium
berghei.
Conclusion: The ethyl acetate leaf extract of C. viscosa was found to be a good
source for the development of new medicine or drug for the treatment of drug resistant
malaria.
Key words: Plasmodium falciparum, Cleome viscosa, antimararial activity,
cytotoxicity, THP-1 cell line, IC50, Plasmodium berghei
Figure 1: Cleome viscose (Whole Plant)
(RACPABS - 2020)
OP-15: OVER EXPLOITATION OF GROUNDWATER AT COASTAL

AREAS AND THEIR IMPACT ON CHEMICAL CHANGES OF
NATURAL ENVIRONMENT
1.Lalitha Vinnakota, A. Anitha And Dr. V. Venkata Rathnamma

2.Department Of Zoology And Aquaculture
Acharya Nagarjuna University, Guntur-522510. A.P, India
Email: Vinnakotalalitha090@Gmail.Com
Environmental chemistry deals with the chemical processes that occur in water, air,
terrestrial and living environments including the effects of human activity on them. Water has
many unique properties, characteristics and is very important for every living organism on
this earth but only 0.61% of freshwater is available in the form of groundwater that found
below the earth’s surface. Over exploitation of groundwater at seashore or coastal areas by
natural processes or human activities by digging more wells for freshwater storage induces
saltwater intrusion due to the hydraulic connection between groundwater and seawater results
in decreasing the groundwater levels or by increasing seawater levels. It is a widespread
environmental problem of coastal regions It can accelerate salinity processes and aggravating
the affected land. Chemical constituents which are present in seawater can deteriorate the
water quality by changing their temperature, pH, salinity, alkalinity, metal concentration, DO,
nutrients and texture. High concentration of sodium and chloride can make water unfit for
human consumption, horticulture, agriculture, fisheries and for industrial uses and it also
contribute to various kinds of diseases to animals and plants. The chemical substance which
enters into the groundwater is very difficult and impossible to remove back; this change
brings marked reduction in vegetation levels and adversely affects their growth. Long term
effects include natural dynamism of affected wet and dry land ecosystems of coastal areas
that are prone to several severe natural hazards. The rate of groundwater extraction is
unsustainable thus it was seen as a threat for future. So withdraw of groundwater shall be
permitted where no other source of water is available and maximum try to look for alternative
water supplies. Protection of the groundwater resources is needed to continue the life on
earth.
(RACPABS - 2020)
OP-16: EFFICIENCY OF STREPTOMYCES SP. AS BIOCONTROL

AGENT FOR MANAGEMENT OF FUNGAL DISEASES IN BLACK
GRAM.
M.Kishore babu, K. Rajyalaxmi, Dr.D.S.D suman joshi, Luay Kadhim Hanoon,

Qasim Turki, Dr. A. Krishna Satya*
Department of biotechnology, Acharya Nagarjuna University, Nagarjuna nagar,
Guntur, Andhra pradesh-522510, India.
Corresponding author e-mail:akrishnasatya78@gmail.com
Approximately 25% of the crop is losing in agriculture lands are affected by

phytopathogens. In most cases, chemical pesticides are commonly used for the effective
control of phytopathogens and pests. They use of chemical pesticides are effective in the
environmental and it causes human health problems. Biopesticides or biological pesticides
are better alternatives that are eco-friendly and equally effective. In the present study
Actinomycetes sp were evaluated for their antagonistic potential against Colletotrichum
lindemuthianum (Anthracnose), Fusarium oxysporum (Fusarium wilt) and Rhizoctonia solani
(Blight disease) in Vigna mungo (Black Gram). A Total of 93 Actinomycetes sps were
isolated from rhizosphere of Black Gram at different places in Guntur district of Andhra
Pradesh, India the antagonistic activity of Actinomycetes sp against Colletotrichum
lindemuthianum, Fusarium oxysporum and Rhizoctonia solani was done by dual culture
assay. Among the 93 isolates, only TVS-2, NVS-10, DVS-11, KZSS-6, ESS-4, and TSS-3
showed maximum inhibition against three plant fungal pathogens. These six antagonistic
potential Actinomycetes sp were further characterized for their plant growth promoting
properties and enzyme production like, Indole-3-acetic acid (IAA) production (18.9 to 82.0
µg ml-1), Siderophore production (24-65.6 units), Hydrocyanic acid (HCN), β- 1, 3-
Glucanases assay (25.9-32.3 units), chitinase, cellulase, lipase and proteases, And also
characterized for their physiological properties i.e., Temperature, pH and salinity, carbon
source utilization and antibiotic production. The 16 S rDNA analysis of TVS-2, NVS-10,
DVS-11, KZSS-6, ESS-4, and TSS-3 showed that all the isolates were matching with
Streptomyces sp.
(RACPABS - 2020)
OP-17: HETEROLOGOUS PRODUCTION OF POLY-EXTREMO

TOLERANT LACCASE FROM HALOPHILIC BACTERIA WITH ITS
APPLICATION IN DYE DECOLORIZATION
Manne Anupama Ammulu, Vinay Viswanath K, Ajay Kumar G, Sudhakar Poda *

Department of Biotechnology, Acharya Nagarjuna University, Guntur, India
Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna nagar,
Andhra Pradesh, Guntur, India
Email id: sudhakarpodha@gmail.com,
The need to explore biodiversity for the production of novel metabolites is increasing
progressively. Bioprospecting is the most frequently used term for screening of biological
resources for the extraction of commercially important compounds. Furthermore, laccases are
also capable of performing polymerization, depolymerization, methylation, and
demethylation reactions. Because of their wide substrate activity, laccases have received
broad interest for their biotechnological applications in paper pulping, dye decolourization,
bioremediation, denim refining, textile cleaning, juice and wine clarification, biosensor and
biofuel cell design, manufacture of new compounded material from lignin waste
detoxification of environmental pollutants, and revalorization of wastes and wastewaters. The
bacterial laccases may have many advantageous properties compared to classical fungal
laccases because of their highly efficient expression, much higher thermostability, and
alkaline torelancy, Based on a survey of biotechnical and enzymatic applications involving
halophilic organisms, it is likely that halolaccases will offer up disruptive technologies where
a high pH, temperature, and salt, metal, and chemical contents and low water content media
are desirable. Despite such stability and the numerous advantages that prokaryotic enzyme
production ,may offer over eukaryotic production. Industrial or biotechnological use of
bacterial laccase is still not viable because of its extremely low yield. Therefore, there is a
need to find novel halo-bacterial laccases with potential industrial relevance through the
exploration of natural diversity and also to improve the yield which can have tremendous
laccase applications. Due to the less diversity of the enzyme isolation in prokaryotes and its
wide range of biotechnological applications, the present investigation aims to isolate and
characterize halo-bacterial isolates, capable of laccase production, from different resources
and to establish optimal physiological parameters for higher concentration of laccase
production from a selected strain and to investigate dye decolorization potential of laccases
thus produced.
(RACPABS - 2020)
OP-18: CHEMICALS AND DRUGS FROM ORCHIDS
Md. Rahamtulla1, J. Ramudu2 and S.M.Khasim1*

1
Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar-
522510, Guntur, Andhra Pradesh, India; 2Department of Botany, V.S.R Govt. Degree and
P.G. College, Movva, Krishna Dt., Andhra Pradesh, Pin: 521135
*Email: prof.smkhasim@gmail.com
The Orchidaceae, one of the biggest of the angiosperm families with more than
763 genera and an estimated 28,000 species, is well-known for its floral complexities. In
India, the orchids are engaged for a variety of therapeutic uses in different systems of
traditional medicine. In the ayurvedic system of medicine, orchids such as Habenaria
intermedia, H. edgeworthii, Malaxis acuminata and M. muscifera are the key components of
Ashtavarga, which is a group of eight medicinal plants employed in the preparation of a
number of rejuvenating formulations and tonics. Other commonly used orchid drugs in the
ayurvedic system are salem (Orchis latifolia, Eulophia latifolia), Jiwanti (Dendobium
alpestre) and rasna (Acampe papillosa). Likewise, many orchid species are used for a variety
of ailments. Ever since the isolation of ‘dendrobine’ from Dendrobium nobile, a large number
of alkaloids and other phytochemicals have been reported from the family Orchidaceae. The
present review work mainly highlights the traditional therapeutic uses of orchids, and
orchidaceous phyto-constituents such as alkaloids, carbohydrates, flavonoids, glycosides,
resins, sterols terpenes and tannins. Further, some of the therapeutic properties and the
important chemicals isolated from orchids are presented in detail.
OP-19: EFFECT OF PHYSICAL PARAMETERS AND NUTRIENT

SOURCES ON DIBENZOTHIOPHENE BIODESULFURIZATION
ACTIVITY AND GROWTH OF THE STREPTOMYCES SP.
VUR PPR 101.
Praveen Reddy. P1 and UmaMaheshwara Rao. V2*

1
Department of Microbiology, Vivekananda Degree and PG College, Vavilalpally,
Karimnagar-505001, Telangana.
2*
Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjunanagar-
522510, Guntur District, Andhra Pradesh.
Globally, fossil fuels (petroleum products and coal) are enormously used by humans
in their daily life. Fossil fuels are composed of a wide range of complex organic compounds.
Oxidation of fossil fuels, during their utilization emits hazardous gaseous pollutants into the
(RACPABS - 2020)
atmosphere. The sulphur dioxide is one among those gaseous pollutants, released as a result
of oxidation of organosulfur compounds occurring in fossil fuels. The sulphur dioxide is
deleterious to humans and one of the major causes of acid rain. The hydrodesulfurization
process, which in current practice for the removal of sulphur content from petroleum products
is not a convincing approach as it is ineffective in the removal of certain highly recalcitrant
organosulfur compounds, especially, dibenzothiophene. An alternative approach suggested
by many researchers is biodesulfurization, which exploits microbes for the elimination of
sulphur from organosulfur compounds present in petroleum products. The dibenzothiophene
is regarded as a model organosulfur compound to carry out biodesulfurization experiments.
The microbes which can remove sulphur atom via 4S biochemical pathway are regarded as
commercial strains as they selectively disassociate sulphur from dibenzothiophene in the
form of sulphite without affecting the ring structure of dibenzothiophene and thus restoring
the calorific value of the petroleum product. The present work was aimed to study the effect
of physical parameters (temperature and pH) and nutrient sources viz., carbon (Glucose,
Fructose, Sucrose, Maltose, Starch, Cellulose, Mannitol and Glycerol), nitrogen (Peptone,
Yeast extract, Casein, Urea, Ammonium chloride, Ammonium sulfate, Sodium nitrate and
Potassium nitrate) and amino acid sources (Proline, Alanine, Phenylalanine, Tyrosine,
Arginine, Lysine, Cysteine, Methionine and Glutamine) on the dibenzothiophene
desulfurization activity and growth of bacterium, Streptomyces sp. VUR PPR 101 isolated
from oil contaminated soils of mechanical workshops. Minimal salt broth with
dibenzothiophene was inoculated with the bacterium and incubated at different temperatures
and pH levels. Similarly, the bacterium was grown in dibenzothiophene containing minimal
salt broth supplemented with different concentrations of the selected nutrient sources to
determine the optimum concentration of each nutrient source required for enhanced
dibenzothiophene desulfurization activity and growth .The results obtained during this
optimization study revealed that 30o C temperature and pH 7.0 of the physical parameters;
3% glucose, 1% yeast extract and 0.3 mg/ml glutamine of carbon, nitrogen and amino acid
sources, respectively were optimum for the enhanced desulfurization activity as well as
growth of the Streptomyces sp. VUR PPR 101.
(RACPABS - 2020)
OP-20: 2-DEOXYGLYCOSYL 3-BENZOYLPROPIONATES AS

NOVELDONORS FOR THE DIRECT AND STEREOSELECTIVE
SYNTHESISOF 2-DEOXY-GLYCOSIDES
Ramakrishna Bandi, Sudharani Chalapala and Srinivasan Chandrasekaran

Lewis acid mediated stereo selective synthesis of 2-deoxy-O-glycosides has been

demonstrated using 2-deoxyglycosyl 3-benzoylpropionates as novel glycosyl donors. These
newly developed donors are easily synthesized from simple glycals, are stable at room
temperature and react with ease to provide products with high stereo selectivity. These donors
can be successfully utilized with all types of acceptors (primary, secondary and tertiary
alcohols) for the synthesis of 2-deoxy-glycosides. Additionally, these newly developed
glycosyl donors are also efficient for the synthesis of tri-saccharides.
OBn
BnO
OBn O Ph OH
BnO O
Sc(OTf) 3 (0.5 eq) BnO
O BnO O OBn
BnO O OBn
BnO CH2 Cl2 O
OBn , rt,
O OMe
O 1.5 h
OBn
OBn
1 2 3 (75% only )
OP-21: A STUDY ON EFFECT OF METAL IONS IN

POLYGALACTURONASE PRODUCTION FROM PENCELLIUM
SPECIES
Ravi Ketipally and M. Raghu Ram*

Department of Botany and Microbiology
Acharya Nagarjuna University, Guntur, Andhra Pradesh-522510
*Corresponding author: mraghuram2002@gmail.com
In the present investigation, two Penicillium species were isolated from mangrove
habitats (Gilakaladhindi) of Krishna district, Andhra Pradesh, India. The isolated strains
showed positive results for polygalacturonase. The pectinase positive cultures were identified
based on cultural, morphological and molecular characterization. The sequence was deposited
in NCBI Gen bank with accession numbers (KU 613360 and KU 613362). Different metal
ions such as, ZnSo4, CaCl2, MgSO4, MnSO4, FeSO4 and CuSO4 were added to the Czapek-
(RACPABS - 2020)
dox medium, and polygalacturonase production was estimated. Further various

concentrations (0.1, 0.2, 0.3, 0.4 and 0.5%) of metal ions were also tested. Zinc supported
maximum enzyme production by the two Penicillium species studied. Among them
Penicillium griseofulvum produced 4.15 U/ml of enzyme while Penicillium citrinum RR101
produced 4.46 U/ml With increase in metal ion concentration an increase in enzyme
production was observed. Maximum enzyme production was observed at 0.3% in Penicillium
griseofulvum and 0.4% in Penicillium citrinum RR101. Polygalacturonase from Penicillium
species are suitable for large scale industries.
OP-22: KNOWLEDGE AND PRACTICE OF EMERGENCE

CONTRACEPTIVES AMONG FEMALE STUDENTS OF EJERE
WOREDA, OROMIA REGION, CENTRAL ETHIOPIA
Tolera Gudissa Dame

Department of Nursing and midwifery, College of Medicine and Health Sciences, Ambo
University, Ambo, Ethiopia.*Email:tolerag@gmail.com
Emergency contraceptive is a type of modern contraception that is indicated after

un-protected sexual practice or due to contraceptive failure. Although Emergency
contraceptive prevent unwanted pregnancy and its consequences like unsafe abortionand
unintended child birth, there is no information about the knowledge and practice of
Emergence contraceptive among young femalesin the study area.The aim of this study is to
assess the knowledge and practice of Emergence contraceptive among female students of
Ejere high school and preparatory school. A facility based cross sectional study design was
conducted among270 Ejere high school and preparatory school female students from April 1-
15 of 2015. A simple random sampling technique was used to select the study subjects until
the required sample size was collected. Then the collected data was analyzed andpresented in
tables, figures and narratives. From the total respondents, about 116 (43%) of the respondents
were replied as they have heard about emergence contraceptives and only 44 (38%) and 14
(22%) of the respondents were knowledgeable and ever practiced emergency contraceptive
respectively. Among those sexually active respondents(23.7%), some of the reasons why they
were not using ECs arelack of knowledge about ECs, no desire to use the methods and due to
religious reasons.The study indicated that the knowledge and utilizationlevel of emergency
contraceptives amongthe study respondents were low. Therefore, Ejere woreda health office
in collaboration with Ejereworedaeducational office should work on awareness creationabout
emergency contraceptives, and on its advantage to prevent unwanted pregnancy and its
damaging consequences after unprotected sex among the young female students.
(RACPABS - 2020)
OP-23: TRANSCRIPTIONAL PROFILING OF ORANGE RIPENING

MUTANT IN TOMATO(SOLANUM LYCOPERSICUM)
P. John Elia Prashanth, N. Rajesh, C. Jareena Begum and P. Osman Basha*

Department of Genetics and Genomics, Yogi Vemana University, Kadapa, YSR District,
A.P., India* Corresponding author: osmanbasha@yahoo.co.in, osmanbasha@gmail.com
One of the ultimate goals of agriculture research is to design beneficial phenotypes to

increase yield and quality of the crop plants and it is achieved through plant breeding over
decades. Genetic variability is a very basic asset for crop domestication and improvement, as
well as genetic research. Fruit ripening, shelf life and yield are important traits in tomato and
most of research targeted these traits. Mutants with altered carotenoid pathway are valuable
and are particularly helpful to understand the complete regulatory pathway, which may help
to attempts to increase the levels of carotenoids by conventional plant breeding or genetic
manipulation methods. Comparison were made between eight samples at transcription level
using Tomato Microarray (Agilent technologies) at Mature Green, Breaker, Turning and Red
ripe stages of orange ripening mutant and wild type cv. Arka Vikas. Ripening-associated
pathway genes including the Phytoene synthase-1, Phytoene desaturase, Lycopene ε-cyclase,
Lycopene β-cyclase, Carotenoid isomerase and transcription factors were evaluated for
expression levels. mRNA of CRTISO was not detected in fruit of Orange ripening mutant
compared to wild type expression. Sequence analysis of ripening-associated pathway and
transcription factors identifies deletion in Carotenoid Isomerase (CRTISO) gene promoter
region at 407bp compared to wild type Solanum lycopersicum cv. Arka Vikas. Genetic
investigation of orange ripening mutant clearly indicated that this mutation monogenic
recessive in nature. The mutant may provide insight into devising gene-based strategies for
enhancing carotenoid accumulation in tomato fruits. Detail biochemical and genetic
investigation of orange ripening mutant (Orr2) may provide insight into devising gene-based
strategies for enhancing carotenoid accumulation in tomato fruits.
(RACPABS - 2020)
OP-24: OUTER MEMBRANE VESICLES OF BOVINE RESPIRATORY

DISEASE ASSOCIATED PASTEURELLA MULTOCIDA AND
MANNHEIMIA HAEMOLYTICA AS PLATFORM IN VETERINARY
VACCINE TECHNOLOGY
*Mirtneh Akalu Yilma (mirtneh2010@gmail.com)
Bovine Respiratory Disease (BRD) is a complex multi-factorial disease causing a

severe form of pneumonia in cattle. The two members of the Pasteurellaceae family,
Mannheimia haemolytica (M. haemolytica) and Pasteurella multocida (P. multocida)
represent major bacterial causative agents of respiratory disease in cattle. Immunization and
treatment controlling approaches are used to minimize the burden of the disease. Currently
used commercial vaccines are inefficacious, 50-70% prevention. Antibiotics treatment is
costly and impractical with a question of antimicrobial resistance. Hence, cost effective
vaccines and therapeutic intervention are indispensible to control the disease. Study of the
outer membrane vesicles (OMV) production under natural and diverse stress conditions has
broadened the horizons, and also opened new frontiers in delineating the molecular
machinery involved in disease pathogenesis, development of effective diagnostic tools and as
vaccines against diverse pathogenic species of Gram-negative origin. OMVs are small
spherical structures (approximately 10-300 nm), which are naturally released from the outer
membrane of Gram-negative bacteria and can be purified from the culture supernatant by
filtration and centrifugation steps. It is enriched with bioactive proteins, toxins, and virulence
factors, and play a critical role in the bacteria-bacteria and bacteria-host interactions. The
immunogenicity of OMV has been evaluated for specific antibody induction, and together
with functional bactericidal and challenge assays in mice has shown their protective potential.
The use of membrane vesicles as a nonliving, acellular vaccine has been studied with several
bacteria. In addition, vesicles can serve as their own adjuvant, which can further decrease
production costs. The outer membrane proteins OmpA, OmpH, and P6 were identified as the
three major immunogenic proteins of P. multocida driven OMVs, while P2 and OmpA were
found to be the most important antigens of M. haemolytica OMVs. Colonization studies
showed the significant cross-immunogenic potential of these OMVs. In general these findings
serve as a platform in veterinary vaccine technology, future development of broad-spectrum
OMV based vaccines against BRD and other infections caused by P. multocida or M.
haemolytica.
(RACPABS - 2020)
OP-25: ISOLATION AND SCREENING OF ANTIBIOTIC PRODUCING

ACTINOMYCETES FROM THE RIFT VALLEY AREAS OF ETHIOPIA
Firew Elias, Sudhamani Muddada and Driba Muelta

Department of Biotechnology, KL University, Guntur, India
Department of Biotechnology, KL University, Guntur, India
Environmental Biotechnology Unit, Institute of Biotechnology,
Addis Ababa University
Infectious diseases caused by antibiotic resistant bacteria lead to considerable increase

in human morbidity and mortality globally. This situation requires more attention to find
solutions by searching and producing new and effective antibiotics. Antibiotics are secondary
metabolites produced by microorganisms. Actinomycetes are the top producers of antibiotics.
The purpose of the present study was design to isolate, and screen actinomycetes isolates that
produce bioactive metabolites from rift valley areas of Ethiopia. Soil samples were obtained
from rhizosphere of plants, agricultural soils and shoreline of which composite soil samples
prepared. Composite soil samples were pre-treated with heat and CaCO3. Soil suspension
(0.1 ml) from 10-5 serially diluted composite soil sample was spread on selective media for
selective growth and isolation of actinomycetes. The primary and secondary screenings of
antibacterial active isolates were done by streak plate and disc diffusion assay, respectively.
A total of 259 actinomycetes were isolated. Out of the 259 actinomycetes,176 (60%) isolates
showed varying levels of antimicrobial activity against the test bacteria. In this study, isolates
RVE129 and RVE187 were found to inhibit all of the test organisms. The isolates were
identified and characterized by cultural, morphological, physiological, and biochemical
characteristics and found to be classified under the genus Streptomycetes. In order to extract
the active compounds from isolates, fermentation was carried out on 24 isolates and the
former was extracted by using ethyl acetate. Finally, isolate RVE129 was selected for further
study based on spectrum activity from disc diffusion assay. The finding of this study, in
general, showed that, antimicrobial compounds obtained from RVE129 demonstrate broad
spectrum antimicrobial activity against S. aureus S. epidermidis-ATCC-12228 S. thypi
ATCC-13311 E. coli ATCC 25922, S. typhi ATCC1402, P. aeruginosa ATCC25853 and K.
pnemoniea, ATCC-700603. Thus, searching effective antibacterial agents from
microorganisms isolated from undiscovered habitat should be given high priority.
Actinomycetes isolated from large unscreened and diverse ecosystem for the isolation of
potent antibiotic producing actinomycetes are the first report in this area. Overall, the study
revealed that the selected actinomycetes recovered from rift valley areas of Ethiopia
presented good candidates to be explored as new sources of bioactive compounds.
(RACPABS - 2020)
OP-26: FISHERIES IN TRANSITION: IMPLICATIONS FOR FOOD

AND NUTRITION SECURITY IN INDIA
Darwin, Ch., P. Padmavathi and Gatreddi Srinu

Department of Zoology & Aquaculture
Acharya Nagarjuna University,Nagarjuna Nagar – 522 510,Andhra Pradesh, India
E-mail: darwin.chatla@gmail.com; padmapin@yahoo.com
India has made considerable progress against human development indicators in recent
years, but malnutrition resulting from poor dietary diversity and low micronutrient intakes
remains entrenched. Surveys show that in the developing countries diets are qualitatively
deficient in nutritional values due to low intake of foods from animal origin. Besides that,
fish has unique advantages when compared to other animal proteins. It is the cheapest source
of quality protein which plays a great role in quenching the nutritional requirement in both
developing and under developed countries. In particular, marine fishes are known to be a
reservoir of essential nutrients like digestible proteins, amino acids, polyunsaturated fatty
acids, vitamins, and trace elements along with other secondary metabolites with bioactive
properties which are essential for holistic nutritional security. In many countries wild capture
fisheries remain the dominant supplier to provide elite group for nutrition security. Whereas
in India, the marine fish supply is undergoing a transition with aquaculture. However, this
transition increased availability of fish from aquaculture but not have fully compensated the
loss of fish from capture fisheries in terms of dietary diversity, micro nutrients and nutrition
security. Hence the present paper reviews the range of approaches that are recommended to
sustain and enhance the contributions from capture fisheries to make ensure the nutritional
security in India.
OP-27: ADVANCED MOLECULAR METHOD IN DISEASE

MONITORING FOR DNA VIRUSES
D.N.V.Raja Rajeswara Rao, Jaganmai.G, K.Sabasteen, Rajeswari Jinka*

Department of Biochemistry, University College of Sciences, Acharya Nagarjuna University,
Guntur, Andhra Pradesh, India.;(jinkarajeswari@gmail.com)
Viruses are associated with a variety of diseases that affect different systems of the
human body. DNA viruses like Hepatitis B Virus (HBV) may cause acute and chronic
diseases which may lead to hepatic carcinomas and antibiotic resistance in humans. Hence
the early and accurate diagnosis of the disease is critically important. Undiagnosed patients
can unknowingly transmit the disease to others. So, early diagnosis can help to prevent or
stop an outbreak.
(RACPABS - 2020)
The current study mainly focussed to identify an infectious disease through molecular
based diagnosis by using Real-time PCR (qPCR). There are several tools used in molecular
diagnostics which include- Fluorescence In-Situ Hybridization (FISH), DNA Microarray,
Sequencing, Spectrometry, Flow-cytometry etc., but the use of Polymerase Chain Reaction in
molecular diagnostics has increased to the point where it is now accepted as the Gold
standard for detecting nucleic acids and has become an essential tool in disease diagnosis.
Real-time PCR has engenerated wider acceptance of the PCR due to its improved rapidity,
sensitivity, reproducibility and reduced risk to carry-over contamination.
Keywords: Molecular diagnostics, DNA Microarray, FISH, Real-time PCR.
OP-28: PRELIMINARY PHYTOCHEMICAL SCREENING OF

ETHANOMEDICINAL PLANTS HOMALIUM ZEYLANICUM AND
SCHREBERA SWIETENIOIDES.
Santosh kumari .M & Ammani.K

Department of Botany,Acharya Nagarjuna University.
Medicinal plants are the reservoirs of potentially useful chemical compounds that have
been serving for ages as theureupetic agents and leads for modern drug design .The main
objective of the present study is to investigate the presence of various phytochemicals from the
whole plant methanolic extracts of the medicinal plants Homalium zeylanicum and
Schrebera swietenioides. The various parts of the plant including root, stem, leaves and bark
are having many traditional uses, mainly in diabetes, leprosy, wound healing ,rheumatism, spleen
enlargement and hydrocele. Phyto chemical screening plays an important role in the detection of
the bioactive compounds. The phytochemical tests of the extracts from whole plant were found
individually to contain alkaloids, tannins, flavonoids, phenolic compounds, triterpenoids, steroids,
glycosides and saponins. The generated data provides the basis for their wide use in traditional
and folk medicine.This can be exploited for new drug design in the future. Results will be
discussed in depth.
(RACPABS - 2020)
OP-29 ENVIRONMENTAL EDUCATION AND SCIENTIFIC

APPROACHES IN SCHOOLS: THE INDIAN SCENARIO
Dr. Sudhakar G 1, Swarna latha G2, Dr. P.Brahmaji Rao3

1
Post doctoral Fellow, Dept. of Environmental sciences, Acharya Nagarjuna University
2
Research Scholar, Dept. of Environmental sciences, Acharya Nagarjuna University
3
Associate Professor, Dept. of Environmental sciences, Acharya Nagarjuna University
Environmental pollutions and social issues are damage the nature and life, to
overcome these problems the honorable Supreme Court of India formed the directive
judgment to introduce the Environmental education (EE) as a regular course in all formal
education system in India, Prior to this directive several programmes were current in the
country related to EE. In schools higher classes have scientific approaches to understand, as
well as one of the program was centrally sponsored scheme "Environmental Orientation to
School Education (EOSE)". Through this program it was expected to orient school children
to its immediate environment using locale-specific examples and materials with scientific
reasons, To do so a range of activities were initiated including production of locale-specific
print materials, training modules, practical activities and so on. The Ministry of Human
Resource Development, Govt. of India had appointed three Nodal Agencies to implement the
Scheme. The present study focus on EE with scientific approaches should practice to identify
and understand the pollution or social problems, the remedy from root level of the
communities because the school students are future generation or social community
Key words: Environmental education, school education, formal education, scientific
approaches
OP-30: DYE YIELDING PLANTS - A TREASURE OF ETHIOPIA

Dr. Anuradha.G1, Dr. Teshome Abdo1, Dr. Alemu Gonfa Robi1, J. Sreekanth Kumar2
1
Assistant Professor, Vice president for Academic Affairs, Director of Center for Excellence,
Adama Science and Technology University, Adama, Ethiopia.
+251901723466, anuradha.g@astu.edu.et, teshomeab@yahoo.com, alemug4@gmail.com
2
Acharya Nagarjuna University, Guntur, A.P, India.
+91 77806 75835, sreekanth.mythri@gmail.com.
Nature is a big source of colours, has been ignored by the mankind for many years.
Though the ancient civilizations utilized the Natural colours, very little light is thrown in this
area in recent times. Over the past many years, it has been observed that synthetic dyes have
many disadvantages like toxicity, pollution and allergenicity despite natural dyes have no
such disadvantages. Natural colourants are used mainly for textile dyeing, painting, glass,
(RACPABS - 2020)
leather, food, beverages, cosmetics and paper industry. Application of natural colourants is
very much restricted then synthetic dyes in terms of market captivity. Ethiopian historical
legacy reflects the use of jubilant colours in their ancient pictures, paintings and archeological
remains probably derived from natural sources. Significant database is not available with
reference to production of colours from natural sources. The colourants can be derived from
leaves, roots, bark, fruits, flowers, trunk of plants and vegetables either in dried or wet form
with tremendous applications and with reduced toxicity. In this article we discuss the use of
dye yielding plants and their application from the land of Ethiopia.
Fig. 1 Natural dyes in ancient manuscripts Fig. 2 Natural dyes in Ethiopian tradition
OP-31: NICKEL STRESS TOLERANCE AND BIOSORPTION

POTENTIAL OF RHIZOBIA ASSOCIATED WITH HORSE GRAM
[MACROTYLOMA UNIFLORUM (LAM.) VERDC.]
Prabhavati Edulamudi1*, Anthony Johnson Antony Masilamani3, Venkata Ramana Sai

Gopal Divi2, Umamaheswara Rao Vanga1, Veera Mallaiah Konada1
1
Department of Botany and Microbiology, Acharya Nagarjuna University, Andhra Pradesh, India
2
Department of Virology, Sri Venkateswara University, Tirupati, Andhra Pradesh, India
3
Department of Botany, Sri Krishnadevaraya University, Ananthapur, Andhra Pradesh, India
E-mail: prabha_anumicro@rediffmail.com.
The rhizobia isolated from root nodules of Horse gram [Macrotyloma uniflorum
(Lam.) Verdc.] were screened for their ability of heavy metal (Ni) tolerance. Heavy metal
tolerance studies revealed that the strain HGR-4 have shown significant growth on Yeast
Extract Mannitol (YEM) agar medium containing 1000 µg g-1 of Nickel (Ni). Horse gram
plants associated with HGR-4 have shown Ni stress tolerance in Ni amended soils up to a
concentration of 100 µg g-1. They have shown higher nodulation, nitrogen level and
leghaemoglobin content at 80 µg g-1 of Ni in comparison to control plants (without rhizobial
inoculation). Analysis of biosorption potential of Ni in Horse gram plants inoculated by the
strain HGR-4 using Atomic Absorption Spectroscopy (AAS) revealed maximum biosorption
(RACPABS - 2020)
in both nodules and soil samples. This study demonstrated that the Horse gram plants
associated with rhizobia besides having nitrogen fixing capacity also have shown Ni stress
tolerance and the ability to remove Ni from soils. The strain HGR-4 (GQ483457 Rhizobium
sp. ATCC BAA-2335) could be used for the phytoremediation in the treatment of Ni
contaminated soils upon its association with Horse gram. The study could be of use in
phytoremediation of metal (Ni) contaminated soils in the future.
OP-32: DEVELOPMENT & VERIFICATION OF LCMS/MS METHOD

FOR DETERMINATION OF N-NITROSO-N-METHYL-4-
AMINOBUTYRIC ACID (NMBA) CONTENT IN VALSARTAN
Dr.Ch.Prasanna1* & Yarram Rama Koti Reddy2

1
Department of Environmental Sciences, Acharya Nagarjuna University, Guntur.
2
Centre for Chemical Science and Technology, IST, JNTU Hyderabad.
Email: prasannachimata7@gmail.com
The aim of the present study was the development and verification of a simple,
Linear, Precise, accurate and specific LCMS/MS method for the Determination of NMBA
(N-Nitroso-N-Methyl-4-Aminobutyric Acid) content in valsartan as per FDA specification
Limits.Simple, sensitive, specific, Precise and accurate LCMS/MS method was developed
and verified for the determination of NMBA content in valsartan, separation was achieved on
a Phenomenex Kinetex F5 (150mmX4.6mm),2.6µ under gradient Mode. The mobile phase
composed of Mobile Phase-A: (0.1% Formic acid in Water) and Mobile Phase B:(0.1%
Formic acid in Methanol). The flow rate was monitored at 0.5 mL/min by using binary pump
ESI, positive mode at m/z 146.90 and standard retention time at 4.159 min. (N-Nitroso-N-
Methyl-4-Aminobutyric Acid).The retention time of NMBA (N-Nitroso-N-Methyl-4-
Aminobutyric Acid) were found at 4.159 min in standard. Linearity was established for
NMBA content was in the range of LOQ level to 200% specification Level. The percentage
recoveries for NMBA content were found in the range of 80% at LOQ & 100 % specification
levels. This method can be successfully employed for quantitative analysis of NMBA content
in valsartan.
(RACPABS - 2020)
OP-33: FUNGAL PATHOGEN ASSOCIATED WITH MURRAYA

KOENIGII L.
1
1
Department of Biotechnology, Government Womens College, Kolar-563101, Karnataka.
2
Vikram B.Ed,. College, Chintamani,Kanataka.
Curry leaf (Murraya koenigii L.) of the family Rutaceae have long been considered a
premier leafy vegetable with attractive habit and graceful leaves. It is used in fresh, dried and
processed forms for flavoring food stuffs. The species is native to India and Srilanka. It is
extensively used in the indigenous system of medicine as an anti-diabetic agent. Further it is
known to possess anti-inflammatory, antioxidant and diverse pharmacological properties. The
plant is grown presently in the backyards of every South Indian home, but its nutritive value
and medicinal properties have not been fully appreciated. A very common disease in the plant
is leaf spot and powdery mildew so it has gained least attention of researchers. Curry leaf
plants with leaf spot and powdery mildew disease were collected from Mandya district of
Karnataka, India. The pathogen was isolated on Potato Dextrose agar (PDA) from surface
sterilized small pieces of the leaves and twigs, incubated at 25°C, and identified as Rhizopus
and Aspergillus was the cause for the disease according to its morphological and cultural
characteristics and study was done based on colony characters.
OP-34: IRISIN- EFFECT ON METABOLISM
Sai Bhavani, Sandeep kumar, Sravanthi Malempati*

Department of Biochemistry, Krishna University Dr MRAR PG Center, Nuzvid
Email:malempati.sravanthi0@gmail.com
Irisin was described as an exercise-induced myokine with a peptide structure of 112

amino acids Hecksteden et al (2013). Irisin is the product of type I membrane protein
cleavage encoded by fibronectin type III domain containing 5 (FNDC5) genes Boström et al.
(2012). Irisin is secreted mainly in skeletal muscle, especially in the perimysium,
endomysium, and nuclear parts, albeit adipose tissue, pancreas, sebaceous glands, and cardiac
muscle have been identified as secretory tissues. Irisin was immunoreactivity in salivary
glands, ovaries, testes, rectum, intracranial arteries, tongue, optic nerve, stomach, neuronal
cells, and sweat glands Aydin (2014).Irisin is a thermogenic protein that promotes energy
expenditure by WAT browning and belongs to the class of adipomyokines since it acts both
in adipose and muscle tissue (adipokine and myokine) Rodriguez et al (2017). Irisin
facilitates glucose uptake by skeletal muscles, improves hepatic glucose and lipid
(RACPABS - 2020)
metabolism, having a positive effect on hyperlipidemia and hyperglycemia caused by obesity

and metabolic syndrome. Outcomes based on the research studies of Moreno-Navarrete et al
(2013) irisin influences the organs and tissues involved in type 2 diabetes, such as the liver
and pancreas, by reducing insulin resistance, but the mechanisms by which it modulates the
function of pancreatic islets are still unknown. Liver lipid metabolism also plays an essential
role in glucose homeostasis. When the intake of carbohydrates exceeds the capacity to store
and oxidize, they are converted into fat by de novo lipogenesis. Considerable evidence has
suggested that ER stress, especially hepatic ER, is closely linked to metabolic diseases, by
promoting glucose production in the liver, lipogenesis and IR in obesity, and diabetes irisin
remains a hope in prevention and treatment of metabolic syndrome, an effect that needs to be
validated in future work. Irisin could be a potential therapeutic target for human metabolic
diseases and other disorders that are improved with exercise. However, further studies are
required for better understanding of its metabolic effects and their possible mechanisms.
OP-35: TERPENOIDS AND THERE CLASSIFICATION
G.Mounika, Sudharani Chalapala and S. Anwar

Plant terpenoids are used extensively for their aromatic qualities. They play a role in
traditional herbal remedies and are under investigation for antibacterial, antineoplastics, and
other pharmaceutical functions. Plants do not only accumulate terpenes for herbivore defense,
but also emit volatile blends in response to herbivory and many other biotic and abiotic
stresses. These terpene containing volatiles attract natural enemies of the attacking herbivores
but due to the complexity of these volatile blends, it is difficult to attribute a specific function
to a particular terpene. The importance of terpenes in both nature and human application is
difficult to overstate. Basic knowledge of terpene and isoprene biosynthesis and chemistry
has accelerated the pace at which scientists have come to understand many plant biochemical
and metabolic processes. The abundance and diversity of terpene compounds in nature can
have ecosystem-wide influences. Although terpenes have permeated human civilization since
the Egyptians, terpene synthesis pathways are only now being understood in great detail. The
use of bioinformatics and molecular databases has largely contributed to analyzing exactly
how and when terpenes are synthesized. The steroids and sterols in animals are biologically
produced from terpenoid precursors. Sometimes terpenoids are added to proteins, e.g., to
enhance their attachment to the cell membrane; this is known as isoprenylation
(RACPABS - 2020)
OP-36: BETTER PROTECTION- NANOFIBERS IN MASKS AND

RESPIRATORS
Murthy, Sarath Kumar, Naga Raju, Sunil, Kishore*

Department of Biochemistry, Krishna University Dr MRAR PGC, Nuzvid
Email: gkrbio@gmail.com
Society needs enhanced filters to remove, protect the people and environment. Recent
developments in nanoscale materials have elevated general public. Nanofibers enhance filter
performance for capture of naturally occurring nanoparticles such as viruses as well as
micron-sized particles such as soot from diesel exhaust. Wells (1930) was the first to
hypothesize droplet nuclei transmission of airborne infections in the 1930s. When a
contagious individual coughs or sneezes, numerous droplets containing infectious particles
(bacteria and viruses) are released Chao et al (2009). A facemask is a loose-fitting, disposable
device that creates a physical barrier between the mouth and nose of the wearer and potential
contaminants in the immediate environment. They are generally labelled as surgical,
isolation, dental or medical procedure masks and personal air purifiers. They are designed to
protect the wearer from inhaling dangerous substances such as toxic chemicals and infectious
particles. Respirators are designed to help reduce the wearer’s respiratory exposure to
airborne contaminants such as particles that are small enough to be inhaled - particles less
than 100 microns in size.
Nanofibers could be the key elements for filter materials in face masks or respirators.
They have a very high surface area per unit mass that enhances capture efficiency and other
surface area-dependent phenomena that may be engineered into the fiber surfaces (such as
catalysis or ion exchange). They could enhance filter performance for capture of naturally
occurring nanoparticles such as viruses, as well as micron-sized particles such as bacteria or
man-made particles such as soot from diesel exhaust.Nanofibers and nanofiber webs, used in
the filtration and separation of sub-micron sized contaminants is a major concern of modern
day nanotechnology. Nowadays in industrial workplaces the rapid growth of nanotechnology
(RACPABS - 2020)
has increased the production of these engineered nanoparticles. Tasks and processes such as
cleaning engineered nanomaterials from dust collection systems, spills, and disposal can
present a potential for inhalation exposure and a high level of risk. Nanofibrous media have
low weight, high permeability and small pore size that make them appropriate for a wide
range of filtration applications especially for smaller particles. In addition, nanofiber
membranes offer unique properties such as high specific surface area (ranging from 1-100 m2
/g depending on the diameter of fibres and intrafiber porosity), good interconnectivity of
pores and potential to incorporate active chemistry or functionality on a nanoscale Barhate, et
al (2008).Nanofibers do not exist independently in filtration, but are produced onto a
substrate, typically a nonwoven fabric. There are four main types of relevant filtration
mechanism using nanofiber filter media. These are surface straining, depth straining, depth
filtration and cake filtration; Hutten, (2016).Nanofibers masks with disposable non-woven
face mask, non-woven mask for operation room, PM2.5, a N95 and a R95 respirator which
efficiently filter out the PM2.5 particles and simultaneously preserve a good breathability.
Filter efficiency increases as the size of the fibers decreases. In this point nanofibers could be
the key elements for filter materials in face masks or respirators. They could enhance filter
performance for capture of naturally occurring nanoparticles such as viruses, as well as
micron-sized particles such as bacteria or man-made particles such as soot from diesel
exhaust. We believe respiratory protective equipment developments require more research on
advanced nanofibers filtration technology can provide the necessary protection from airborne
threats.
OP-37: PHYTO-CHEMICAL STUDIES AND EVALUATION OF

ANTIOXIDANT PROPERTIES OF SELECTED MANGROVE
EXTRACTS
Deepthimahanthi Divya1, G. Beulah2, K. Govinda Rao2, N.S. Sampath Kumar3 and

G. Simhachala2*
Lecturer in Zoology, St. Anns College for women, Hyderabad, Telangana, India
Department of Zoology and Aquaculture, Acharya Nagarjuna University, Guntur, A.P., India
Department of Biotechnology, Vignan’s University, Guntur, A.P., 522213, India
* Corresponding Author
The free radicals are highly reactivity species with very short half-life and damaging
activity towards macromolecules like proteins, DNA and lipids. Dietary plants contain
variable amounts of antioxidants that prevent the oxidation and prolong the life of the
oxidizable matter. The present study was undertaken to determine phytochemical (phenolic
and flavonoids) content and free radical scavenging ability via DPPH radical scavenging
(RACPABS - 2020)
assay and Reducing ability assay of selected mangrove leaf extracts Acanthus ilicifolius,
Avicennia marina, Bruguiera cylindrical, Halosarca indica, Rhizophora mucoronata,
Sesuvium portulacastrum, Suaeda martima, Exocoecaria agallocha collected from
Machilipatnam region. Tested extracts showed better DPPH radical scavenging ability at
varying concentration from 102.94 - 373.63 μl/ml compared to the ascorbic acid. Among the
tested plants, the leave extract of Acanthus ilicifolius and Suaeda martima were found to
possess high antioxidant activity. With rigorous investigation on various mangrove plants it
can be anticipated that the natural antioxidants are going to replace the synthetic antioxidants
in the near future.
OP-38: REMEDIATION OF ENVIRONMENTAL POLLUTION—ROLE

OF NANOPARTICLES
Mounika, Krishna Tara, Kalavathi, Ammulu, Vardhan*

Department of Biochemistry, Krishna University Dr MRAR PG Center, Nuzvid
Email:svm2032@gmail.com
Environmental pollution is undoubtedly one of the main problems that society facing
today. Various new technologies are constantly being explored for the remediation of
contaminants of the air, water, and soil. Particulate matter, heavy metals, pesticides,
herbicides, fertilizers, oil spills, toxic gases, industrial effluents, sewage, and organic
compounds are just a few examples of the many concerning contaminants. The unique
properties of Nanomaterials’ allow removing pollutants from the environment are extremely
small size, typically billionth of a metre, creates a large surface area in relation to their
volume, which makes them highly reactive, compared to non-nano forms of the same
materials. Silver, iron, gold, titanium oxides and iron oxides are some of the commonly used
nanoscale metals and metal oxides. (AgNPs) Silver nanoparticles are significant antibacterial,
antifungal, and antiviral activity, and thus applied as water disinfectants. Silver nanoparticles
of less than 10 nm in diameter were found to be highly toxic to Escherichia
coli and Pseudomonas aeruginosa and can also prevent viruses glycoproteins from binding to
host cells. Triangular AgNPs exhibiting better antibacterial effects than Ag nanorods and Ag
nanosphere.Carbon based nanomaterials are also employed to remediate contaminants
through photocatalytic approaches promote nanotubes to the generation of valence band holes
(h+). Surface treatments, activation, or functionalization of the pristine carbon material as
Carbon nanotubes; multi-walled & single walled carbon nanotubes significantly used for
environmental remediation applications. The adsorption properties of these materials make
them particularly useful for the removal of organic and inorganic pollutants from air and from
large volumes of aqueous solution (Kharisov et al (2014), Ren et al., (2011). Graphene oxide
(RACPABS - 2020)
(GO) is an example of modified graphene that has been described for environmental
remediation by adsorption of a variety of gaseous and water contaminants, such as SOx, H2S,
NH3, volatile organic compounds, heavy metals, pesticides, and pharmaceuticals Wang et al
(2013). Nanoparticle such as Ag doped Tio2; Cu/Fe/Ag-doped TiO2; Gold coated with
citosan polymer, Carbon nanotubes/ Al2O3 models that demonstrate efficient removal of some
pollutants were 2,4,6- Trichlorophenol; nitrate; Zn2+, Cu2+ form aqueous solutions; fluoride
Fernanda et al (2018). In addition to remediating pollution, they can be used as sensors to
monitor toxins, heavy metals and organic contaminants in land, air and water environments.
Therefore, nanomaterials due their powerful potential applications to reduce remediation of
contaminants will increase at a great leap in the near future, and it will play a critical role in
sustainable development. Much research is necessary to elucidate the fate of these materials
after introduction to the environment for remediation purposes in order to avoid the
possibility of these materials becoming themselves a source of environmental contamination.
OP-39: FFECT OF ETHION (50%EC) ON ENZYMATIC ACTIVITIES

OF SDH AND LDH IN FRESHWATER FISH LABEO ROHITA
(HAMILTON)
Dr.Ch. Prasanna,
Department of Environmental Sciences, Acharya Nagarjuna University, Guntur-522510, A.P,
India. Email:prasannachimata7@gmail.com
Water is an essential for life and to human existence. Organic organisms are sensitive
to pesticide chemicals and toxic concentrations of excessive spillage of agricultural activities
and several other resources. The ability to predict the effects of pollutants or chemicals on
organisms and to estimate toxicant effects from laboratory to population and community
levels has become a very important factor. The Indian major carp Labeo rohita was exposed
to lethal (1.2µg/l) and sub-lethal (1/10th of lethal) concentrations for 24hrs, 5th and 10th days
of Ethion (50%EC) to examine the SDH and LDH enzymatic activity, in different tissues like
gill, liver, kidney, brain and muscle and control group were also maintained in all the
exposure periods.SDH prevents the transfer of electron to molecular oxygen, resulting the
inhibition of SDH activity and shifting to aerobic metabolism to anaerobiosis.LDH plays a
very important role in carbohydrate metabolism Lactate convert to Pyruvate. The SDH and
LDH enzymes activity levels are increased in all tissues of subleathal concentrations when
exposed 5th and 10th days when compare with controls.
The SDH activity levels in control fish were in order of: Liver>Kidney>Muscle>Gill>
Brain and subleathal: Liver>Kidney>Brain>Muscle>Gill. The activity of LDH in control
fish: Liver>Kidney>Brain>Muscle>Gill, and subleathal: Liver>Kidney>Muscle>Gill>Brain.
(RACPABS - 2020)
OP-40: DNA BARCODING TECHNOLOGY: IMPLICATIONS FOR

SEAFOOD TRACEABILITY AND AUTHENTICITY
Gatreddi Srinu, P. Padmavathi and Ch. Darwin

Department of Zoology and Aquaculture
Acharya Nagarjuna University, Nagarjuna Nagar – 522 510, Andhra Pradesh, India
E-mail: gatreddisrinu@gmail.com; padmapin@yahoo.com
Food traceability and authenticity is a primary concern for enhanced safety in trading
and consumer confidence to ensure right and quality food due to socio-economic and health
implications. Identification of commercially mislabelled or deliberately substituted species is
a major challenge in seafood trade industry especially in case of processed raw materials, i.e.
those modified by milling, drying, or extraction, which move through various stages before
reaching the finished product. DNA-based approaches are increasingly acknowledged as
powerful tools for assessing the reliability of seafood labelling of various processed and
semi-processed products. In this direction, DNA barcoding technology based on Cytochrome
oxidase I (COI) was established as a promising technology for sea food traceability and
authenticity. Seafood certification using this technique is an easy, rapid, accurate and cost
effective technology that helps in getting the right food by the consumer. Existence of various
DNA barcode reference libraries (BOLD, NCBI-GenBank, CBOL, Fish-BOL, DDBJ,
EMBL) across all regions of the globe are being maintained by various international agencies
helps in correct identification of species at rapid pace even by a non-taxonomist. In this
paper, the data of several researches are critically analyzed and reviewed in order to exploit
the effectiveness of DNA barcoding in food traceability, and to delineate some best practices
in the application of DNA barcoding. This review highlights the problems raised by the
mislabelling with reference to economic fraud and conservation of threatened species as well.
OP-41: Syzygium aqueum for better health and welfare of mankind
Kethipalli Swapna*, Kethipalli Ravi1 and Babitha Bobba *

*Department of Foods and Nutritional sciences
1
Department of Botany and Microbiology;Acharya Nagarjuna University,
Nagarjuna Nagar, Guntur, A.P., India;Email: babithbobba@yahoo.co.in
Consumption of diets containing fruits and vegetables has been an essential means of
living a healthy life because of the essential bioactive compounds. Fruits and vegetables can
reduce the risk of stroke and cancer because antioxidant contained in the plant parts. Recent
days fruits and leafy vegetables become a crucial part of scientific research these foods itself
(RACPABS - 2020)
can be used as remedy for most of the diseases. Although chemicals, drugs and different
medicines are used for treating various diseases, most of the drugs show side effects, which
can be sometimes fatal to the end user. Syzygium aqueum also known as watery rose apple or
water apple. Water apple, is one of the underutilized and less studied fruit in India. It is
grown on domestic level mostly in the backyard or in the kitchen garden. Commercial
plantations of Water Apple are rarely seen in India. Recently due to greater awareness and
passion about health and nutrition, people are exploring more dietary options. Watery Apple
is one such fruit getting attention in Indian fruit markets. Water apple has thirst relieving
properties and is consumed raw. This fruit is reported to have health benefits for patients,
suffering from diabetes. Fruits, leaves and bark of this fruit has many medicinal uses like
fever treatment, detoxify the liver, headaches, lower cholesterol, skin conditions and
prevention against certain types of cancers.
OP-42: HEPATOPROTECTIVE EFFECT OF INDIGENOUS

FOENICULUM VULGARE IN ETHANOL INTOXICATED MALE AND
FEMALE ALBINO RATS
Vinodhini Rajamanickam
Unit of Biochemistry, Department of Medicine, College of Medicine and Health Sciences,
Ambo University, P.O. Box. 19, Ambo, Ethiopia.
Email: vinobchem@gmail.com
The liver performs many vital functions to eliminate toxins and harmful substances
from the body. Hepatotoxic agents can directly interact with the liver through the formation
of reactive radicals and consequently induce liver lesions with the progression of alcoholic
liver disease. The aim of this study was to investigate the possible hepatoprotective effect of
Foeniculumvulgareagainst ethanol induced hepatotoxicity in albino rats. The male and female
rats with an average body weight (245 – 255g) were divided into ten groups as (Group 1 –
Group 5) males and (Group 6 – Group 10) females in each group (n = 6) respectively. The
rats were intoxicated with 30% of ethanol for 30 days. At the start of 31st day the rats (males:
Group 4 – Group 5 and females: Group 9 – Group 10) were treated with 50 mg/kg and 1 g/kg
of very fine powder of F. vulgaresuspended in PBS. The males (Group 3) and Females
(Group 8) were treated with suspension of Silymarin (50 mg/kg) for 30 days. At the end of
experimental period all the animals were sacrificed to isolate the liver and blood samples.
The biochemical markers such as alkaline phosphatase (ALP), alanine transaminase (ALT),
aspartate transaminase (AST), total and direct bilirubin are increased in alcohol intoxicated
groups. Supplemented with F. vulgarein the treatment period decreased and stabilized the
concentration of all parameters in male and female rats. The reduction in total protein and
(RACPABS - 2020)
albumin levels of alcohol intoxicated groups were significantly increased in F. vulgaretreated

male and female rats. The results of F. vulgareare in consistent with the Silymarin and control
rats. This study confirmed the hepatoprotective effect of F. vulgarein alcohol intoxicated
male and female albino rats.
OP-43: SYNTHESIS OF SILVER NANOPARTICLES FROM

PSEUDERANTHEMUM MALABARICUM AND EVALUATION OF ANTICANCER
ACTIVITY.
Luay Kadhim Hanoon, M.Kishore babu, Qasim Turki, K. Rajyalaxmi, Dr.

A. Krishna Satya*
Department of biotechnology, Acharya Nagarjuna University, Nagarjuna nagar, Guntur,
Andhra pradesh-522510, India. Corresponding author e-mail:akrishnasatya78@gmail.com
Silver salts occupy the predominating position in usage since the start of the
civilization but the synthesis of silver nanoparticles using various sources is a recent
development. Hence, the present study was focused on eco-friendly and green approach
synthesis of silver nanoparticles using plant extracts of P. malabaricum. The morphological
and physical properties of synthesized silver nanoparticles were analyzed using various
spectroscopic and microscopic studies such as UV-Visible spectroscopy, FT-IR, XRD, SEM
with EDAX, DLS, Zeta potential and the Particle size analyzer. After structural
characterization, the synthesized silver nanoparticles were subjected to anti-cancer activity
studies against cervical cancer cell line i.e., HeLa cells using MTT assay.
OP-44: Methylene blue degradation by green synthesized Silver

Nanoparticles from Oldenlandia corymbosa
K.Raghavendra1,V.UmaMaheswaraRao2,R.Chinthayyanaidu3,K.V.A.Naidu1
K.Suryanarayana3,M.Rajeswari3,J.HariHindhuja3 ,J.Dinesh3,T.Pavani3, B.Akhila3
1
Dept. of Biosciences,RGUKT,Nuzvid
2
Dept. of Botany & Microbiology, Acharya Nagarjuna University, Guntur
3
Dept. of Chemical Engineering,RGUKT,Nuzvid
Sunlight is an abundantly available natural source of energy which can be

conveniently exploited for the photodegradation of pollutants and can make the process
economically more viable.
(RACPABS - 2020)
In this present study the silver nanoparticles AgNPs are prepared from Oldenlandia
corymbosa, a flowering plant in the family Rubiaceae. [1].The synthesized AgNPs are used
to study photocatalytic activity [2] on Methylene Blue (MB) a synthetic dye released in
effluents from textile industries.In aqueous medium MB shows an absorption bands at 664
nm with a shoulder at 614 nm.The 5mg of prepared 0.1M AgNPs are added to 50 ml of
0.001% (w/v) Methylene Blue and kept in the sunlight. It’s UV Vis spectra is recorded with
a time interval of 15min.A sharp decrease in absorbance over time along with change of MB
color from Blue to light Blue confirms its degradation.
Further photodegradation studies have to be done with azo dyes like methyl orange,
congo red, methyl red, and methyl yellow which are toxic and non biodegradable.
0.001% MB MB solution after Decrease in MB absorbance with

solution photodegradation 15min time interval
OP-45: GREEN SYNTHESIS OF SILVER, COPPER AND ZINC

NANOPARTICLES BY USING PODRANEA RICASOLIANA (TANF.)
SPRAGUE LEAF EXTRACT
K. Raghavendra1 and V. Umamaheswara Rao2

1Dept. of Biosciences, RGUKT, Nuzvid
2 Dept. of Botany and Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar,
Nanoparticles are microscopic particles with a diameter range between 1 to 100 nm.
These particles possess physical properties such as high conductance, uniformity and special
optical properties which make them ideal for use in biology and materials science [1].
Nanoparticles synthesis has been achieved through various routes, but Green synthesis [2]
has been regarded as a safer alternative to usual physical, chemical and microbial methods
due to its cost-effectiveness, environment-friendly nature and easy handling.In the present
study, firstly reporting an eco-friendly, bottom-up and facile method for biogenic synthesis of
(RACPABS - 2020)
Silver (Ag), Copper (Cu) and Zinc (Zn) Nanoparticles (NPs) using leaf extract of Podranea
ricasoliana (Tanf.) Sprague (PLE) with their respective metal salt precursors. Podranea, a
vulnerable native ornamental plant in South Africa and cultivated in Asia, belongs to
Bignoniaceae family. The formation of Silver, Copper and Zinc nanoparticles of PLE was
primarily determined based on the change in color of the respective metal salts precursors,
from colorless to wine red, blue to green and colorless to brown, respectively. The prepared
Silver, Copper and Zinc nanoparticles are characterized by UV–Vis spectroscopy with
respect to their surface plasmon resonance (SPR) values. Further characterization has to be
done with Fourier Transform Infra Red spectroscopy (FTIR) for functional groups involved
in biosynthesis process, X-Ray Diffraction (XRD) for particle size and crystallite characters,
XRF to distinguish the Cu & CuO/ Zn & ZnO nanoparticles, Dynamic Light Scattering
(DLS) for particle size and stability and Transmittance Electron Microscopy (TEM) or
Energy-Dispersive X-ray Spectrum (SEM-EDS) for physical shape and inorganic elemental
analysis. The work on antimicrobial activity and photo catalytic activity of the silver, copper
and zinc nanoparticles of PLE is in progress.
Pondranea ricasoliana and its Colors of silver, copper Changed colors of silver,
leaf extract (PLE) and zinc salt solutions copper and zinc salt solutions
before adding plant extract after formation of
nanoparticles with plant
extract
(RACPABS - 2020)
OP-46: RECENT ADVANCES IN NANO-BIOTECHNOLOGY FOR

IMPROVEMENT IN THERAPEUTIC APPROACHES FOR THE
TREATMENT OF DIABETES
Dr. T. Sujatha,
Head of Dept. of Chemistry, Sri Sarathi Institute of Engineering & Technology,
NUZVID, Krishna District, A.P
Diabetes mellitus is a chronic metabolic disorder which results in elevated blood

glucose. It can be broadly classified as Type 1 and Type 2, depending on the reason for the
high blood glucose levels. In Type 1 diabetes enough insulin is not produced by pancreatic
beta cells, whereas in Type 2 the body's cells poorly respond to the presence of
insulin. Nanotechnology holds a great deal of promise in diabetes management for efficient
drug delivery, generation of smart drugs which only activate when needed. Anti-diabetes
therapy is significantly transformed by using nanotechnology in many ways like in delivery
of therapeutic molecules, fabrication of nanosensors to monitor dynamic changes in blood
glucose level, and development of smart imaging molecule to visualise and quantify beta
cells etc. This review article explores the way in which nanotechnology could be used to
effectively treat, and possibly cure, diabetes. We anticipate in the long run, nanotechnology
will clearly open up many routes to treatments and participate in understanding and finding a
cure that would improve the lives of all people affected by diabetes.
OP-47: APPROACH TOWARDS SOLID WASTAGE MANAGEMENT
B. Satish Babu1, N. Chimpiraiah2, Dr. P. Brahmaji Rao3

1&2
. Research Scholar, Dept. of Environmental Sciences, Acharya Nagarjuna University.
3.
Associate Professor, Dept. of Environmental Sciences, Acharya Nagarjuna University.
Municipal wastes in India are about 75 to 80% organic. Uncontrolled dumping of

waste is major health concern. Degradation by use of microbes is safe, efficient and
economic. Enzymatic secretion by microbes as concern with biodegradation is an important
strategy to overcome over waste related problems. Samples were collected from dumping
ground and inoculated on biodegradable waste such as kitchen waste, garden waste and
temple waste. The obtained inoculums where treated with biodegradable waste. After 45 to
60 days degraded compost where identified for several parameters such as physical,
biochemical and chemical. The compost produced had a dark colour and volume of waste
was produced. Amylase, protease and cellulose secretion of isolates indicate the ability to
(RACPABS - 2020)
degrade waste. PH, N, organic carbon and moisture content where identified which are
required to enhance soil fertility.
Key Words: Municipal Solid waste, Degradation, Microbial isolates, Waste Management,
Soil fertility.
OP-48: Green Synthesis of Silver Nanoparticles using Orthosiphon

stamineus (Benth) leaf extract
N. Vijaya Sree*, P.Udaya Sri* & N. RamaRao#

*Department of Biotechnology, Acharya Nagarjuna University, Guntur-522510,
#
Principal, Chalapathi Institute of Pharmaceutical Sciences, Lam, Guntur-34,
For Correspondence Email: vijaya_1410@yahoo.com
Today, nanotechnology represents a promising approach to improve agricultural

production, waste management and environmental remediation. Green nanotechnology
focuses on challenges within the nanoscale that need to be overcome to ensure eco-friendly
processes and products. Itinvolves the use of less energy during manufacture, ability to
recycle after use, using eco-friendly materials. Green synthesis is a harmless and
environment-friendly method for producing nanoparticles. The use of environmentally benign
materials like plant leaf extract, bacteria, fungi and enzymes for the synthesis of nanoparticles
offers numerous benefits of ecofriendliness for waste management and environmental
remediation and compatibility for pharmaceutical and other biomedical applications as they
do not use toxic chemicals for the synthesis protocol. This study aims to investigate the
Methanolic extracts of the leaves of Orthosiphon stamineus for the presence of various
phytochemicals and to synthesize silver nanoparticles using the methanolic extract of
Orthosiphon stamineus leaves. The Methanolic extracts of the leaves of Orthosiphon
stamineus were investigated for the presence of phytochemicals such as polyphenols,
alkaloids, terpenoids, flavonoids, carbohydrates, steroids and steroids using standard
biochemical methods. The synthesis of silver nanoparticles was analyzed by UV-Visible
spectroscopy, Scanning Electron Microscopy. Stable silver nanoparticles were formed by
treating aqueous solution of AgNO3 with the plant extract as reducing agent of Ag+ to Ag0.
UV-visible spectroscopy was used to monitor the quantitative formation of silver
nanoparticles. The synthesized silver nanoparticles were characterized with inductively
coupled plasma spectrometry (ICP), energy dispersive X-ray spectroscopy (EDS), scanning
electron microscopy (SEM), transmission electron microscopy (TEM), and particle analyzer.
Key words: Orthosiphon stamineus; Nanoparticles; Green synthesis.
(RACPABS - 2020)
OP-49: Structural investigation of the exopolysaccharide produced by

Heavy metal resistant Rhizobium radiobacter strain
*Pallaval Veera Bramhachari

Assistant Professor, Department of Biotechnology, KRISHNA UNIVERSITY,
Machilipatnam - 521 001- AP, India
Phone: 08672-225963; Fax No: 08672-225960; Mobile No : 8897019580
E-mail-veerabramha@gmail.com
Increasing attention has been given, over the past decades, to the production of
exopolysaccharides (EPS) from Rhizobium radioabcter, due to their various biotechnological
applications. Overall characterization of biopolymers involves evaluation of their chemical,
physical, and biological properties; this evaluation is a key factor in understanding their
behavior in different metal exposed environments, which enables researchers to foresee their
potential applications. Production of exopolysaccharides by Rhizobium has been linked with
efficient nodulation of leguminous plant roots by the bacteria in presense of toxic metal
polutants. The exopolysaccharides produced were analyzed for polysaccharide composition
by column chromatography and thin-layer chromatography. Further, structure of
polysaccachride was confirmed by HPLC, FT-IR and NMR spectroscopy. Based on this
spectroscopy results, the polysaccharide produced by Rhizobium sp. was 3 linked β-D-
galactopyronosyl units. The results suggested a heteropolymer structure for these EPS
composed by mannose and galactose as prevailing monomer unit has an approximate
molecular mass of 750 kDa. Knowledge of the Rhizobal - EPS composition now facilitates
further investigations relating polysaccharide structure and dynamics to rheological
properties.
(RACPABS - 2020)
OP-50: MANGROVE –MEDIATED SYNTHESIS AND

CHARACTERIZATION OF SILVER NANOPARTICLES USING
ACANTHUS ILICIFOLIUS PLANT LEAF EXTRACT.
Sunila Rani Pudota, Gorrepati Rosaiah *, Nattala Tirupati Swamy,

Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur,
Andhra Pradesh, India-522510.
*Corresponding author: gorrepati_rosaiah@yahoo.co.in
The world of material is rapidly progressing with new technologies and obviously
novel applications in different fields. Nanotechnology is one among these modern and
sophisticated advanced technologies, which is creating waves in the modern times. The
development of eco-friendly and non-toxic processes for the synthesis of nanoparticles is one
of the most important discussed issues in nanotechnology science. The synthesis,
characterization and application of biologically synthesized nanomaterials are in important
aspects in nanotechnology. Biological synthesis of silver nanoparticles has received a
tremendous attention and has been a focus of research due to their high chemical, thermal
stability and promising applications in medicinal field due to its environmental friendly
approach and also low cost techniques. This study reports the green synthesis of silver
nanoparticles (AgNPs) using aqueous extract of leaf, Acanthus ilicifolius, the native
mangrove plant of Machillipatnam - Andhra Pradesh. AgNPs were synthesized by using
1mM AgNO3 solution mixed with leaf aqueous extract. The extract of leaves yielded the
maximum synthesis of AgNPs. Synthesized AgNPs were investigated using UV–visible
spectrophotometry, Scanning Electron Microscopy (SEM), X-ray diffraction (XRD), energy-
dispersive spectroscopy (EDS), Fourier transform infrared (FTIR) spectroscopy and Gas
chromatography and Mass spectroscopy (GC-MS) analysis. Absorption spectrum in 420 nm
confirmed the synthesis of AgNPs. The results of SEM image showed nanoparticles with a
particle size of 62.13 nm. The XRD pattern of biosynthesized AgNPs suggested that the
particles are in crystalline nature. EDS results confirmed the presence of elements like silver,
carbon, oxygen, silicon, and calcium in the nanoparticles produced from leaf extract. Silver
had the maximum percentage of formation, 41.7%. FTIR indicated the presence of different
functional groups such as amines, alcohol, alkaloids, phenol, nitro compounds, and aromatic
loops in the synthesis process. The Phytochemical compounds present in the leaf extract
through GC-MS analysis showed 10 different bioactive compounds. Green biosynthesis of
AgNPs using aqueous extract of native A. ilicifolius appears rapid, reliable, nontoxic, and
eco-friendly. These results not only provide a green approach, for the synthesis of
nanoparticles but also pave a way for new pharmaceutical leads.
Keywords: Nanotechnology; Biological synthesis; mangroves; Acanthus ilicifolius; Silver
nitrate; Silver nanoparticles; aqueous extract; Bioactive compounds.
(RACPABS - 2020)
OP-51: Characterization and Bioactivities evaluation of herbal

nanoparticles mediated by the Leaves of Tabebuia argentea Bur.
& K. Sch.
Nattala Tirupati Swamy, Gorrepati Rosaiah*, Kakumanu Babu and P. Sunila Rani
Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur,
Andhra Pradesh, India-522510.
*Corresponding author: gorrepati_r@yahoo.co.in
Recently nanotechnology has become an essential research field of modern material

science. Herbal nanoparticles have gained large interest owing its innate features like haste,
eco-friendly and cost-effectiveness. Fist time, herbal nanoparticles were successfully
synthesized using Tabebuia argentea leaf powder through Ball mill apparatus in the present
study. The synthesized herbal nanoparticles were characterized by Ultra Violet-Visible
Spectrophotometer, X-ray diffraction (XRD), Scanning Electron Microscopy (SEM) and
Fourier-Transform Infrared Spectroscopy (FTIR). The UV-Vis spectral range between 200nm
to 1200nm was used to study the optical properties of nanoparticles. The X-ray diffraction
studies confirmed that the herbal nanoparticle particles synthesized size in the nano-scale
range of 48.32 nm. SEM results revealed the presence of amorphous nature of the obtained
nanoparticles in the homogenous leaf powder. The FTIR spectrum peaks identified various
functional groups such as alcohol, alkanes, alkynes, amides, esters, nitro compounds, alkyl
halide, ether, sulphone, halogen and aromatic compounds. Synthesized herbal nanoparticles
exhibited diuretic and cytotoxic properties. T. argentea showed more diuretic index (2.81)
and diuretic activity (0.92) at 200 mg/kg dose concentration in vivo studies. It also exhibited
profound cytotoxicity against the MCF 7 cell lines with very less IC50 value of 14.11μg
followed by cell viability percentage of 36.35% at 100 μg concentration in vitro conditions.
So such plant mediated nano-carriers because of their profound biological activities can be
used in advanced drug delivery systems.
Key words: Tabebuia argentea, UV-Vis Spectroscopy, XRD, SEM, FTIR and MCF7.
(RACPABS - 2020)
 CHEMICAL
PP-1: REDUCED GRAPHENE OXIDE (RGO) SUPPORTED PD-RU

BIMETALLIC NANOCOMPOSITE FOR HIGH PERFORMANCE
SUPERCAPACITOR APPLICATIONS
Y.Veera Manohara Reddya, M. Venua, N. Guru Prakash b, B. Sravani c, O.M. Hussain b,

M. Nagalakshmi Devammad, K.Anitha e, P.Saraswathi, G.Madhavi*a
a
Electrochemical Research Laboratory, Department of Chemistry, Sri Venkateswara University, Tirupati,
b
Thin Film Laboratory, Department of Chemistry, Sri Venkateswara University, Tirupati, India
c
Nanoelectrochemistry Laboratory, Department of Chemistry, Yogi Vemana University, Kadapa,
d
Department of Botany, Sri Venkateswara University, Tirupati, India
e
Department of Chemistry, S.K University, Anthapuram, India
e
Department of Physics, S.D.G.S Degree & P.G. College, Hindupuram, Anthapuram, India
Corresponding author email: - gmchem01@gmail.com, gmadhavi@svuniversity.edu.in
Recently, nanocrystalline novel metals combine with carbonaceousmaterial is a

potential electrode material for energy storage applications. However, the size and shape of
nanocrystallites play a predominant role in obtaining high electrochemical performance.
Hence, the investigations were made to synthesizer rGO supported Pd-Ru bimetallic
nanocomposite using hydrothermal method. The morphological, structural and
electrochemical properties were studied. From TEM studies confirm that the rGO decorated
with Pd-Ru nanoparticles are with an average grain size of below 10 nm. XPS measurements
were performed to analyze the surface composition and valance state of the nanocomposite.
The survey XPS spectrum showed that Pd, Ru and C coexist in the prepared rGO/Pd-
Runanocomposite. The electrochemical experiments were carried out in 1M Na2SO4 aqueous
solution and the rGO/Pd-Ru nanocomposite electrode showed the pseudocapacitive nature.
The as prepared rGO/Pd-Ru nanocomposite electrode demonstrated a high discharge specific
capacitance of 705 F/g with good cycling stability. The high specific capacitance of newly
fabricated rGO/Pd-Ru nanocomposite electrode is mainly associated with the synergetic
effect between Pd-Ru nanoparticles and rGO layers and also motivates the future
investigation for the other bimetallic nanocomposites to fabricate the supercapacitor
electrodes.
(RACPABS - 2020)
PP-2: STUDIES ON THE INTERACTIONS OF 1,2,4-TRIAZINES

THROUGH INVERSE DEMAND AZA-DIELS-ALDER REACTIONS
VERSUS ARYNE MEDIATED DOMINO PROCESS AS PROMINENT
FLUORESCENT MATERIALS.
Aluru Rammohan1,2* and Grigory V. Zyryanov1

1
Department of Organic and Biomolecular Chemistry, Ural Federal University, 19 Mira Str.,
Yekaterinburg, 620002, Russian Federation.
2
Department of Chemistry, Sri venkateswara University, Tirupati-517502, AP, India
*E-mail: rammohan4ever@gmail.com
The in situ generated aryne based synthesis of traizines [1,2] is always very attracting
attention in synthetic organic chemistry. Because, this aryne-based methodology is well
recognized as a convenient, one-pot synthetic to a great number of compounds with broad
spectrum of biological activities, alike antiviral [3], antimalarial, cytostatic [4], etc., as well as
their unique fluorescent properties [2]. In the present study, various 5,6-disubstituted-1,2,4-
traizines was treated with anthranilic acid in organic solvents, we got ID aza-Diels-Alder
products (isoquinolines) and domino products (pyrido[1,2-a] indoles) in different ratio of
yields. In case of some reactions, the products were either isoquinolines or pyridoindoles but
not both, based on substitution pattern in starting moiety 5,6-disubtitute-traizine and
anthranilic acid.
Traizine R1 R2 Pyridoindole Isoquinoline

(yields) (yield)
1a Me Ph-Me 2a (42%) 3a (11%)
1b OMe Ph-OMe 2b (39%) 3b (22 %)
1c F Ph-F 2c (42%) -
1d Me CN 2d (9 %) 3d (38%)
1e Me C2F5 2e (12 %) 3e (36%)
(RACPABS - 2020)
PP-3: GREEN SYNTHESIS OF DIETHYL((2-IODO-4-

(TRIFLUOROMETHYL) PHENYL)AMINO)(ARYL)
METHYL)PHOSPHONATES AS POTENT Α-GLUCOSIDASE
INHIBITORS
Mahammad Sadik Shaik1, Maheshwara Reddy Nadiveedhi1, Suresh Reddy Cirandur1*

1
Department of Chemistry, Sri Venkateswara University, Tirupati-517 502, A.P., India.
*E-mail : csrsvu@gmail.com
Novel diethyl ((2iodo4(trifluoromethyl) phenyl) amino) (aryl)methyl) phosphones

(4a-j) were synthesized via a simple and efficient one pot by three-component condensation
reaction (Kabachnik-Fields reaction) [1] of 2-iodo-4-trifluoromethyl aniline, aromatic
aldehydes and diethyl phosphite in presence of anatase TiO2 nanoparticles [2] as catalyst
under solvent free conditions. The molecular docking studies [3] of synthesized compounds
with α-glucosidase enzyme revealed that these compounds have strong α-glucosidase
inhibitory activity. The synthesized compounds (4a-j) are also screened for in vitro
α-glucosidase inhibitory activity and the results showed compound 4i as the strongest
inhibitor and compounds 4a, 4b, 4f and 4g as stronger inhibitors even better than the
reference standard acarbose.
(RACPABS - 2020)
PP-4: STUDY ON PARTIAL MOLAR ISENTROPIC

COMPRESSIBILITY FOR BINARY MIXTURES OF
PROPIOPHENONE WITH 1-ALKANOLS AT VARIOUS
TEMPERATURES 303.15K, 308.15K, 313.15K AND 318.15K
Nanduri Gayatri Devi 1 , D.Bala2 ,D.Ramachandran3

1
Ch.S.D.St.Theresa’s(A) College for Women, Eluru.
2,3
balajivishy@yahoo.comPh.No.+919492702645
Densities ’ρ’, Ultrasonic speeds of sound ‘u’ of binary mixtures of Propiophenone

(PPH) with 1- Propanol, 1-Butanol and 1-Pentanol were measured over the entire
composition range at temperatures from 303.15K to 318.15K and at atmospheric pressure
0.1MPa. From the experimental data, excess isentropic compressibility KsE, partial molar
,
compressibility (PMC) K , , K , , excess partial molar compressibility K , ,
, , ,∞ , ,∞
K , over the whole composition range and K , , K , at infinite dilution
were calculated. The excess isentropic compressibility values correlated using Redlich-Kister
polynomial equation. The variation of these parameters with composition and temperature of
the mixtures have been discussed in terms of molecular interactions in these mixtures. The
observed trend in excess isentropic compressibility values indicates the presence of specific
interactions between Propiophenone and 1-alkanol molecules. It is observed that excess
isentropic values depend upon the number of hydroxyl groups and alkyl chain length in these
binary mixtures.
Study on excess thermodynamic and transport properties of the binary mixtures is
essential to understand more on the molecular interactions. Mixing of the various compounds
with different and complex molecular structure causes various intermolecular interactions,
resulting in non-ideal behavior [Error! Reference source not found.-3].
(RACPABS - 2020)
PP-5: Synthesis and Biological Evaluation of Amide Derivatives of

Imidazopyridine as Anticancer Agents
V. Ramesh a*, G. Purna Chander Raoa, D. Ramachandrana, and A. Kalyan

Chakravarthyb
a
Department of Chemistry, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India.
b
Dr.Reddy's Laboratories, Integrated Product Development, Bachupally, Hyderabad, India.
A series of new amide derivatives of imidazopyridine is synthesized and structures of

the products are confirmed by 1H and 13C NMR, and mass spectral data. The synthesized
derivatives are screened for their anticancer activity against four human cancer cell lines:
lung cancer (A549), breast cancer (MCF-7), melanoma cancer (A375), and colon cancer (HT-
29). Six synthesized compounds exhibit more potent activity than the control drug.
Keywords: Nocodazole (1), Celebrex (2), pyrazole and anticancer activity
Scheme 1. Synthetic pathway to furan sulphonamide derivatives 10a–10j.
R = H (9a,10a),3,4,5-trimethoxy (9b,10b), 4-methoxy (9c,10c), 4-chloro (9d,10d), 4-bromo

(9e, 10e), 4-fluoro (9f,10f), 4-nitro (9g,10g), 4-cyano (9h,10h), 4-methyl (9i,10i), 4-
trifluoromethyl (9j, 10j).
(RACPABS - 2020)
PP-6: DESIGN, SYNTHESIS AND ANTICANCER EVALUATION OF

NOVEL FURAN SULPHONAMIDE DERIVATIVES
G. Purna Chander Raoa*, V. Ramesha, D. Ramachandrana, and

A. Kalyan Chakravarthyb
a
Department of Chemistry, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India.
b
Dr. Reddy’s Laboratories, Integrated Product Development, Bachupally, Hyderabad, India
A series of novel furan sulphonamide derivatives 13a–13j was synthesized. The

structures of the compounds are confirmed by 1H and 13C NMR, and mass spectra. All newly
synthesized compounds wereevaluated for their anticancer activity against three human
cancer cell lines, including breast cancer cell (MCF-7), lung cancer cell (A549) and
melanoma cancer cell (A-375). The compounds 13a–13c, 13e, 13g, 13j demonstrated the
activity higher than the positive control (Doxorubicin).
Scheme 1. Synthetic pathway to furan sulphonamide derivatives 13a–13j.
R = phenyl (12a, 13a), 4-chlorophenyl (12b, 13b), 4-bromophenyl (12c, 13c), 4-fluorophenyl
(12d, 13d), 4-methoxyphenyl (12e, 13e), 4-nitrophenyl (12f, 13f), 4-methylphenyl (12g, 13g),
4-trifluoromethylphenyl (12h, 13h), 3, 5-dichlorophenyl (12i, 13i), 5-bromothiophenyl (12j,
13j).
(RACPABS - 2020)
PP-7: Green synthesis, characterization and application study of Zinc Nano

particles synthesized using aqueous root extract of Sphagneticola
trilobata Lin
Sk.Abdul Mathin1*, M.David Raju2, D.Rama Sekhara Reddy3

1
Department of Basic Sciences and Humanities, Gudlavalleru Engineering College,
Gudlavalleru- 521356, AP.
2
Department of Chemistry, P.B.Siddhartha College of Arts and Sciences, Vijayawada-521010, AP.
3
Department of Chemistry, Krishna University, Machilipatnam-521001, AP.
*Corresponding author: abdulmathin786@gmail.com
In the present investigation, we have described the green biosynthesis of Zinc

nanoparticles (ZnNPs) using aqueous root extract of Sphagneticola trilobata Lin as capping
agent. Scanning Electron Microscopy analysis (SEM) results confirmed the irregular shape of
ZnNPs with different sizes of the particles and an average from 65-80 nm. X-ray diffraction
(XRD) result confirmed the characteristic peaks indicated at 111, 200, 221 and 311 crystal
planes for the crystalline of the face centered cubic lattice. Further, aqueous root extract and
ZnNPs were evaluated to assess their antioxidant potential using various in vitro systems
such as using 1,1-diphenyl-2-picryl-hydrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzothiazoline-
6-sulfonic acid) (ABTS), Ferric reducing antioxidant (FRAP) Assay and Hydroxyl radical
scavenging activity. The anti-diabetic and anti-inflammatory activity of the aqueous root
extract and ZnNPs were also studied and the results confirm the Zinc nano particles
synthesized using aqueous root extract of Sphagneticola trilobata Lin were found to having
enhanced pharmacological activities.
(RACPABS - 2020)
PP-8: DETERMINATION OF GENOTOXIC IMPURITY 2,3-XYLIDINE

IN PARACETAMOL AND MEFENAMIC ACID GFOS SAMPLES
USING RP-LC
Dr. D. Ravi Kumar1, Dr. V. Siva Ramakrishna3, Dr. R.S.K.Sharma1, T.Benjamin2 and
Dr. D. Ramachandran3
1
Department of Chemistry, Krishna University, Dr.MRAR PG Centre,Nuzvid-521201,
2
Department of Chemistry, Noble College, Machilipatnam, A.P., India.
3
Department of Chemistry, Acharya Nagarjuna University, A.P., India.
Email:drkumar.orgchem@gmail.com
Highly sensitive method for the determination of genotoxic impurity such as 2,3-
Xylidine in Paracetamol and Mefenamic acid GFOS samples using RP-LC has been
described in the present paper. Quantification of 2,3-Xylidine content in Paracetamol and
Mefenamic acid GFOS sample by HPLC with UV Detector. 2,3-Xylidine was determined by
RP-LC method using Inertsil ODS-3V(250 x 4.6mm, 5µ) column as stationary phase.
Column temperature maintained 40°C, Injection volume 25µL, flow rate was 1.0 ml.min-1,
sample cooler temperature ambient, 2,3-Xylidine was detected using UV detector at the
wavelength of 230 nm and run time was 40mintues. The mobile phase-A(pH 6.50 phosphate
buffer) and mobile phase -B (methanol and water in the ratio of 90:10 v/v) in gradient
mode. Literature survey indicates that few HPLC methods for the simultaneous assay of
drugs selected under investigation [1-5] and there was no RP-LC method reported for the
determination ofgenotoxic substances in Paracetamol and Mefanamic acid formulations. The
method validation has been carried as per International Conference on Harmonization
guidelines (ICH). Limit of quantitation (LOQ) was found 0.08ppm for 2,3-Xylidine.
(RACPABS - 2020)
PP-9: DEVELOPMENT AND VALIDATION OF A METHOD FOR

SIMULTANEOUS ESTIMATION OF DAUNORUBICIN AND
CYTARABINE IN RAT PLASMA BY LC-MS-MS AND ITS
APPLICATION IN BIOEQUIVALENCE STUDY

Department of chemistry, Acharya Nagarjuna University, Nagarjuna nagar- 522510 .
A unique method for both HPLC & MS/MS assay was developed for the series of
changes in Daunorubicin and Cytarabine in the plasma of rat. For this a mobile phase of 0.1%
Triethyl amine of pH-2.5 adjusted with Ortho Phosphoric Acid and Acetonitrile in (80:20%)
ratio was used and a positive mode of electrospray ionization detection was carried out using
MS. An isocratic mode using Waters X-Bridge C18 (150x4.6mm, 3.5µm) column was used.
The method was validated with a linear range 0.88-17.6ng/ml of Daunorubicin and 2-40ng/ml
of Cytarabine. The %CV values of intraday, interday precision and accuracy were found to be
within the acceptance criteria. The % recovery of Daunorubicin was 99.5% and Cytarabine
was 98.6% respectively. Using liquid liquid extraction, both the drugs were extracted from rat
plasma. From freeze thaw, auto sampler, bench top and long term stability studies, we found
that the drugs were stable throughout the stability studies. According to United States Food
and Drug administration guidelines, the liquid Chromatography Tandem Mass Spectrometry
method was validated successfully. The pharmacokinetic research of Daunorubicin and
Cytarabine in rat plasma was utilized successfully using LCMS/MS.
PP-10: VISCOSITY STUDIES ON BINARY LIQUID MIXTURES OF

PROPIOPHENONE WITH 2-ALKOXYETHANOLS
AT T = (303.15 TO 318.15) K
Nanduri Gayatri Devi 1 , P.Bharath2 ,D.Ramachandran3

1
Ch.S.D.St.Theresa’s (A) College for Women, Eluru.
2,3
balajivishy@yahoo.com; Ph.No.+919492702645
Densities and viscosities of binary mixtures of Propiophenone with 2-

Methoxyethanol, 2-Ethoxyethanol & 2-Butoxyethanol were measured over the entire
composition range at T = (303.15 - 318.15) K and at 0.1Mpa atmospheric pressure.
Experimental data used to calculate excess molar volume (VE), deviation of viscosity Δη,
excess Gibb’s free energy G*E activation of viscous flow for each binary system and the
(RACPABS - 2020)
results were fitted to the Redlich-Kister polynomial equation to obtain the fitting coefficients
and standard deviations. Viscosity values used to compute single adjustable interaction
parameters from Grunberg and Nissan, Katti and Chaudhri, Hind et al. and Tamara Kurata
relations. Deviations in thermodynamic properties of the binary mixtures were discussed in
terms of their molecular interactions between the components. Viscosity data correlated with
the McAllister’s three body /four body models, Auslander, and Jouyban-Acree relations for
two and three adjustable parameters for the studied binary mixtures. Viscosity relations like
Kendall-Monroe, Bingham, Arrhenius, and Kendall were used to compare and calculate the
standard percentage deviation, σ(%), between the experimental and calculated viscosity data.
The studied systems shown weak specific intermolecular interactions and the percentage
deviations were in good agreement with the experimental values. Obtained results are useful
in various chemical and industrial processes. Propiophenone is used as a component in some
perfumes and as an intermediate in the synthesis of pharmaceutical and other organic
compounds [1]. Alkoxyethanols are used as additives to gasoline due to their octane
enhancing and pollution reducing properties [2]. Alkoxyethanols find wide use as important
industrial solvents such as scrubbing liquids in the cleaning of exhaust air and gas streams
from industrial production plants. Therefore the study of alkoxyethanols in pure as well as in
aqueous mixtures is significant from both the theoretical and industrial points of view [3].
PP-11: VOLUMETRIC BEHAVIOUR OF BINARY LIQUID MIXTURES

CONTAINING ALCOHOLS
Sk.Haseena Begum1 and K.Bhagya Lakshmi2
1Dept. of Chemistry, Acharya Nagarjuna University, Guntur, A.P
2Dept. of Chemistry, V.S.R. Govt. Degree and P.G. College, Movva, A.P
The Volumetric effects involved in the binary mixtures of normal and branched
alcohols and various components (Aromatics, Halo and Nitro Aromatics & Halo Aliphatics),
some primary alcohols (Methanol, Ethanol, n-propanol & butanol) with the molecular
interactions of unlike molecules. Molecular interactions should be considered to know their
behavior at different mole fractions at different temperatures. This leads to the determination
of physical parameters like density, viscosity and ultrasonic velocity. Literature inspection
provides so many experimental methods for parameters determination. This experimental
data of mixtures were used to calculate the excess molar volumes and viscosity deviations.
These values can be substituted in the Redlich-Kister polynomical equation for correlation.
This review is a condensation of research activities developed in recent years as results of a
better understanding of volumetric behavior and a stronger insight into the complex structure
of those mixtures. This article also provides several applications of the different combinations
of binary liquids containing alcohols.
(RACPABS - 2020)
PP-12: SIMULTANEOUS DETERMINATION OF AMLODIPINE AND

IRBESARTAN IN FORMULATIONS USING RP-HPLC METHOD
T.Naga Raju1, D.Ravi Kumar1 and D.Ramachandran*

1
Department of Chemistry,Krishna University-Dr.MRAR PG Centre,Nuzvid-521201.
*Department of Chemistry,Acharya Nagarjuna University, N.Nagar-522510.
Email:nagarajutalam@gmail.com
A simple, accurate validated RP- HPLC method for the simultaneous determination of
amlodipine[1] and irbesartan[2] in formulations was developed. Literature survey reveals that
very few analytical methods have been reported for the determination of amlodipineand
irbesartan in combination [3]. The separation of amlodipine and irbesartan was made on
Waters X-Bridge C18 column (250 x 4.6mm, 5μ) using the mobile phase (methanol,
acetonitrile and 0.05M phosphate buffer (pH 4.0 ) in the ratio of 45:25:30 %v/v/v) at a flow
rate of 1.0mL/min with UV detection at 250nm, and the injection volume was 20μL
respectively. Amlodipine and Irbesartan eluted at 2.290 and 5.883 min respectively. The
method is validated within the concentration range of 2.5 – 15μg/mL for amlodipine and 50 –
300 μg/mL for irbesartain with correlation coefficients of r2= 0.9996 and 0.9992 respectively.
The method was validated as per ICH guidelines.The proposed RP-HPLC method can be
used for the routine assay of above mentioned drugs in their tablet formulations.
PP-13: TEMPERATURE DEPENDENT STUDY OF

THERMOPHYSICAL PROPERTIES OF BINARY MIXTURES OF NVP
WITH VARIOUS IMIDAZOLIUM BASED IONIC LIQUIDS
V.Visalakshammaa, T.S.Krishnab,* D.Ramachandranc

a
Department of Chemistry, P.B.Siddhartha College of Arts & Science, Vijayawada – 520010
b
Department of Physics, P.B.Siddhartha College of Arts & Science, Vijayawada – 520010,
c
Department of Chemistry, Acharya Nagarjuna University,Nagarjuna Nagar-522510
Andhra Pradesh, India; E-mail: sritadikonda@gmail.com
The experimental values of densities () and speeds of sound (u) of the binary
mixtures of N-Vinyl-2-pyrrolidinone (NVP) with 2-alkoxyalkanols (2-methoxyethanol, 2-
ethoxyethanol and 2-propoxyethanol) at 298.15-323.15 K have been used to calculate the
excess molar volumes, VmE , excess isentropic compressibility, sE , excess speeds of sound,
E
u E , excess molar isentropic compressibility, Ks,m were calculated and fitted with Redlich-
(RACPABS - 2020)
E E
Kister equation. The excess partial molar volumes, Vm,1 and Vm,2 and excess partial molar
E E °E °E
isentropic compressibilities, K s,m,1 and K s,m,2 , excess partial molar volumes, Vm,1 and Vm,2
E °E
and excess partial molar isentropic compressibilities, K s,m,1 and K s,m,2 of the components at
infinite dilution have also been measured. The results have been interpreted in terms of
intermolecular interactions between the component molecules in the mixture.
PP-14: SYNTHESIS OF BISMUTH MOLYBDATE VIA CO-

PRECIPITATION METHOD AT DIFFERENT PH CONDITION AND
EVALUATION OF CATALYTIC ACTIVITY FOR DEGRADATION OF
METHYLENE BLUE DYE UNDER VISIBLE LIGHT IRRADIATION
B.Lavakusa1*, K.Basavaiah2, D.Srinivasarao3

1
Department of Chemistry, V.K.R College, Buddhavaram, Gannavaram -521101, A.P., India.
*2
Department of Inorganic & Analytical Chemistry, Andhra University,
Visakhapatnam - 530003, A.P., India.
3
Department of Physics, V.K.R College, Buddhavaram, Gannavaram -521101, A.P., India
Corresponding Author : lavakusa99@gmail.com.
Synthesis of bismuth molybdate at different pH conditions as 1.5, 3.0, 5.0 has been
successfully by co-precipitation method and followed by calcination using muffle furnace
with the stoichiometry ratio of bismuth nitrate penta hydrate [Bi(NO3)3 .5H2O], nitric acid
(HNO3), ammonium molybdate tetra hydrate [(NH4)6Mo7 O24 .4H2O] as as precursor
materials. The synthesized bismuth molybdate nanoparticles characterized by X-ray
diffraction (XRD) for identifying crystalline phases and particle size, Raman spectroscopy
identifies active species during the reaction, Fourier transform infrared spectroscopy (FTIR)
in catalysis is to identify adsorbed species and to study the way in which these species are
chemisorbed at the surface of the catalyst, Thermogravimetric analysis (TGA) for thermal
stability of synthesized material, UV-Visible diffuse reflectance spectroscopy (UV-DRS)
revealed for band energy of semiconductors, Field emission scanning electron microscopy
(FE-SEM) is to determine morphology and shape of supported particles and Energy
dispersive X-ray analysis (EDX) is for elemental analysis of synthesized nanoparticles. The
catalytic activity of synthesized bismuth molybdate (pH 1.5,3.0,5.0)catalyst evaluated using
the degradation of methylene blue dye from pollutant water under Visible light irradiation at
room temperature.
(RACPABS - 2020)
PP-15: DETERMINATION OF GENOTOXIC IMPURITY HYDRAZINE

IN URSODEOXYCHLOIC ACID USING RP-LC
Dr. D. Ramachandran* Dr.V.Siva Ramakrishna

*Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar,
Highly sensitive method for the determination of genotoxic impurity such as

hydrazine in ursodeoxychloic acid using RP-LC has been presented in the present paper.
Quantification of hydrazine content in ursodeoxychloic acid sample by HPLC with UV
Detector. Hydrazine was UV inactive compound. Derivatization procedure was established to
detect the hydrazine in HPLC. For this benzaldehyde was used as a derivatizing agent which
reacts with hydrazine in the presence of Disodium tetraborate to form a compound which was
UV active. Hydrazine was determined by RP-LC method using LiChrospher 100-RP18
(250X4.6mm, 5µm) column as stationary phase. Column temperature maintained 35°C,
Injection volume 50µL, Flow rare was 1.0 ml/min, sample cooler temperature ambient,
hydrazine was detected using UV detector at the wavelength of 350 nm and run time was
45mintues. The mobile phase used water and methanol in gradient mode. The method
validation has been carried as per International Conference on Harmonization guidelines
(ICH). Limit of quantitation (LOQ) was found 0.28ppm for hydrazine.
(RACPABS - 2020)
PP-16: FLUORESCENT CHEMOSENSOR FOR VARIOUS METAL

IONS
Eswar Pagadala,Shiva Krishna Loke,Ravi K. Kottalanka*and V. Srinivasadesikan*

Division of Chemistry, Department of Sciences and Humanities,
Vignan’s Foundation for Science, Technology and Research, Guntur,
Andhra Pradesh- 522 213, India.
Email: ravikottalanka@gmail.com, vsdgun@gmail.com
Recent days, a number of fluorescent chemosensors for several metal ions, anions and
amino acids have been designed, synthesized and investigated by chemists. From the
inception of chemosensor, wide applications have been developed in the fields of chemistry,
biology, environmental science, and physiology. In the current study, we have synthesized
furan based Schiff base as chemosensor to detect the metal ions at a various concentration in
water, vegetable, biological system etc., The sensing power of fluorescent sensor of metal
ions will be compared with the results existing in the literature. The synthetic scheme with
the yield has been reported here. The chemosensor applications towards metal ions will be
revealed in due course. Moreover, to understand the metal ion binding behaviour with the
fluorescent sensor the state-of-the-art density functional theory will also be carried out to
support the on-off mechanism.
rt
(RACPABS - 2020)
PP-17: MOLECULAR INTERACTIONS IN BINARY MIXTURES

CONTAINING N-BUTYL ACETATE WITH AROMATIC KETONES AT
VARIOUS TEMPERATURES
A Venkatesh1, M. Radha Sirija2, B. Hima Bindu3, G.Sireesha4,

D. Ani Manjeera5, D.Ramachandran*
,1,2,3,4,5,*
Email: dittakavirc@gmail.com
Densities, viscosities and ultrasonic velocities of binary mixtures containing N-butyl

acetate with Aromatic ketones were measured at various temperatures. From experimental
data, excess parameters were calculated. The obtained values were fitted to the Redlich-
Kister equation. The parameters of excess functions are found sensitive for the intermolecular
interactions between the binary mixtures. Theoretical values of viscosity of the binary
mixtures were calculated using empirical relations and theoretical equations. The relative
merits of these relations and theories were discussed.
PP-18: DESIGN AND SYNTHESIS OF DIFURANBASED

FLUORESCENTCHEMO-SENSOR FOR SELECTIVE DETECTION OF
Fe+3 IONS
Shiva Krishna Loke, Eswar Pagadala, Ravi K. Kottalanka*and V. Srinivasadesikan*

Division of Chemistry, Department of Sciences and Humanities,
Vignan’s Foundation for Science, Technology and Research, Guntur,
Andhra Pradesh- 522 213, India.
Email: ravikottalanka@gmail.com, vsdgun@gmail.com
The aim of colorimetric and fluorescence sensors forthe detection of different species,
such as metal cations,anions, and neutral species, has become a major area ofinterest in recent
years due to their significant roles inbiology, industry, and the environment.In specific,the
designing of a sensor for detecting transition metalions has received significant attention
because the transitionmetal ions lead to criticalenvironmental and health
complications.Among the essential heavy metals, Fe in thehuman body plays a vital role in a
number of physiological activities.The World Health Organization (WHO)suggested that the
average daily iron intake from foods and supplements is 16.2 -18.5mg/day.Iron-deficiency
anaemia can cause serious health problems.Hence, an effectivemethod for the selective
detection of iron ions even at lower concentrationswith high accuracy is desirable.Several
(RACPABS - 2020)
approaches,
ches, including electrochemical assays,inductively coupled plasma mass spectrometry
andatomic absorption spectrometry have been reported which are complicated and expensive.
However,fluorescence chemosensors have a number ofadvantages such as low cost, hig high
sensitivity andselectivity. Considering the importance of Fe (II)/Fe(III) ions in the
biologicalsystems and environment, particular efforts havebeen made to developfuran based
fluorescence on-off chemo-sensors
sensors for sensing Fe ions in iron containing biolo
biological systems
and surface/ground water.Difuran based ancillary ligands can be easily synthesized andthey
can effectively coordinate with a number of metal ions. In this presentation we will discuss
the synthesis of difuran dithiolane (C17H20O2S4)chemo-sensor or and its binding&sensing
affinity towards various transition metals such as Fe, Co, Cu and Zn.The characterization was
carried outbyusing UV–Visible,
Visible, fluorescence titrations & NMR studies.
PP-19: NEW RP-HPLC

HPLC METHOD FOR THE SIMULTANEOUS ESTIMATION OF
ARTEMETHER AND LUMEFANTRINE IN COMBINATION DRUG PRODUCTS

*┼Department of Chemistry, R V R & J C College of Engineering, Chowdavaram,
Guntur, Andhra Pradesh – 522019.
This abstract illustrate an precise, isocratic RP-HPLC

RP HPLC strategy have been created by
the creator for the concurrent estimation of artemether and lumefantrine in unadulterated and
advertised details by utilizing Inertsil C18 column (250×4.6 mm, 5μ) improved mobile phase
containing phosphate buffer(pH 4.5) and acetonitrile in the extent of 40:60 %v/v and
discovery wavelength at 218nm. The retention times were 2.207min and 3.733min for
artemether and lumefantrine respectively. The linearity range was found to be 10-30µg/ml for

522510, Guntur
Guntur, A.P. 177
(RACPABS - 2020)
artemether and 20-60µg/ml for lumefantrine individually. The developed method was
validated for specificity, system suitability, precision, linearity, accuracy, Limit of Detection,
Limit of Quantification, robustness, and Stability and the examine results acquired for all the
approval parameters by this proposed strategy were in reasonable concurrence with ICH
standards. Thus, the developed RP-HPLC method represents another good alternative for the
already existing HPLC methods especially those using certain types of detectors which are
not present in most of the laboratories.
PP-20: ESTIMATION OF PIRACETAM AND CITICOLINE IN

TABLETS BY RP-HPLC METHOD
Meka Srinivasa Rao* & Dr.K.Rambabu┼

*┼Department of Chemistry, R V R & J C College of Engineering, Chowdavaram, Guntur,
Andhra Pradesh – 522019
In this paper a simple, accurate and precise RP-HPLC method for the simultaneous
estimation of piracetam and citicoline in synthetic mixture has been developed and validated.
Separation of drugs was carried out using buffer and Acetonitrile with proportion of 60:40
%v/v as mobile phase at 5 min. run time and 265nm. The Rt value for piracetam and
citicoline was found to be 3.158 and 5.196 min respectively. The developed method has been
validated for linearity, accuracy and precision, LOD, LOQ and system suitability according
to ICH guidelines. The low values of LOD and LOQ illustrate that the developed method was
sensitive, accurate and precise as it can detected and quantify with very low concentration.
The low % RSD values below 2 indicate that the method is precise. The above validation
studies revealed that method is specific, rapid, reliable, and reproducible. The high recovery
and low relative standard deviation confirm the suitability of the method for routine
determination of piracetam and citicoline in combined dose products.
(RACPABS - 2020)
PP-21, VALIDATED REVERSED-PHASE-HIGH PERFORMANCE

LIQUID CHROMATOGRAPHIC ASSAY METHOD FOR
FUROSEMIDE AND SPIRONOLACTONE IN GENERIC
COMBINATION DRUGS
P.Sivannarayana* & Dr. K. Rambabu┼

*┼Department of Chemistry, R V R & J C College of Engineering, Chowdavaram,
Guntur, Andhra Pradesh – 522019.
In the present paper a simple, specific, and validated reversed-phase-high

performance liquid chromatographic assay method was reported for furosemide and
spironolactone in fixed combination dose using C18 column (Chromosil- 250 mmx4.6 mm
I.D; particle size 5µm) with mobile phase consisting of methanol, water and 0.1% Tri ethyl
Amine (pH-4.5) in the ratio of 75:15:10 (%v/v/v) at a flow rate of 1.0ml/min with UV
detection of 237nm in ambient temperature. Furosemide and spiranolactone were
successively eluted at the retention times 3.721 and 4.469min respectively. Regression
analysis revealed linear calibration curves within the concentration of 5.0-15µg/mL
(r2=0.9996) and 10-30µg/ml (r2=0.9999) for furosemide and spironolactone respectively.
Statistical results Student’s t-test for accuracy and F-test for precision revealed no significant
difference between the developed RP-HPLC assay method and the literature method at the 95
% confidence level with respect to accuracy and precision. As the validation results were
within the limits of International Conference on Harmonization guidelines (ICH) it is
concluded, that the developed RP-HPLC assay method can be employed for ordinary quality
control analysis by pharmaceutical companies.
PP-22: VOLUMETRIC, ACOUSTIC AND SPECTROSCOPIC STUDY

OF DIETHYLENE TRIAMIME-AMINO ETHYL ETHANOLAMINE
WITH ALCOHOLS
Dr. D.Rama Chandran department of chemistry , ANU, Guntur, AP, India.

Mrs.Shaik Gousia research scholar in ANU, Guntur, AP, India.
Densities and speeds of sounds for binary mixtures diethylene triamime-emino ethyl
ethanolamine with alcohols were measured at 288.15K, 298.15 & 308.15K over the entire
range of compositions at atmospheric pressures using an antonparr DSA 5000 density meter.
The experimental Densities and speeds of sounds have been used to calculate excess molar
volumes and isentropic compressibility. speed of sound data are compared with theoretical
speed of sound calculated using several approaches.
(RACPABS - 2020)
PP-23: THERMODYNAMIC, TRANSPORT AND AUCOSTIC

PROPERTIES OF BUTYL ACETATE WITH SOME ALCOHOLS AT
DIFFERENT TEMPERATURES
U.Vidyamani, Dr.D.Ramachandran
Department of chemistry, KVR, KVR&MKR College, khajipalem, Acahrya Nagarjuna
University, Guntur-522329, AP, India.
Email:vidyauppalapati@gmail.com
The objective of this investigation is to study the variations of ultrasonic Velocity,

density and viscosity and various acoustic and thermodynamic parameters.
Mehods: The binary mixtures of butyl acetate with ethanol, propanol, butanol, pentanol and
hexanol containing different ultrasonic properties have been studied at different temperature
of 303.15K and 313.15K at the frequency range from 0.5 to few mega hertzs. The ultrasonic
related physical parameters like velocity (U), density (ρ), viscosity (η), adiabatic
compressibility (βad), free volume (Vf), intermolecular free length, are determined.
Results: The results of these studies may also be used for examining the suitability of these
mixtures for other practical applications such as in paints, varnishes, printing ink industries,
bio-medical engineering, textile industry, leather industry and pharmaceutical industry. In
recent years sound velocity measurements are very useful in the technology of polymers.
Conclusion: This work can be effectively utilized as a qualitative study to predict the extent
of molecular interactions between the components.
PP-24: VALIDATION OF A HEAD SPACE GAS

CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF
ETHYLENE OXIDE CONTENT IN DIPYRIDAMOLE API
Sagi.Sambamurthy Raju, Gachakayala.Naresh Babu, Dunna.Sivaram

Department of PG Chemistry, KVRKVR&MKR College, Khajiapelm, Acharya Nagarjuna
University,Guntur-522329, AP, India; Email.smsrajusagi@gmail.com, Email-
lnigchrm@gmail.com,Email-sivarambellamvaripalem@gmail.com
To provide quality control over the manufacture of any API, it is essential to develop
highly selective analytical methods. Gas chromatography with Head space (HSGC) is widely
used for the determination of residual impurities and solvents in API’s. In the current article
we are reporting the development and validation of a rapid and specific Head space gas
chromatographic (HSGC) method for the determination of Ethylene oxide in Dipyridamole
(RACPABS - 2020)
API. The developed method was validated in terms of Specificity, Linearity, Precision,
Accuracy, Limit of detection (LOD) and Limit of quantitation (LOQ) and Precision and
Accuracy at LOQ. The developed method was utilized for the investigation of Ethylene oxide
content in bulk drug.A single, rapid and highly selective HSGC method was developed and
validated for the quantification of Ethylene oxide present in Dipyridamole API through an
understanding of LOD, LOQ, and nature of stationary phases of columns. In linearity study
the correlation coefficient values observed were more than 0.99.The residue Ethylene oxide
was determined in ppm levels also. The method was shown to be specific for Dipyridamole
API and was applied successfully to monitor and control impurity level. Further to the
validation study, three batches of Dipyridamole were analyzed for the content of Ethylene
oxide and the results showed that the Ethylene oxide was absent in all the three batches.
Hence, after studying the data obtained from the validation activity and analysis of three
batches, this Method was applicable for the routine analysis of the Dipyridamole API in
pharmaceutical industry.
PP-25: DEVELOPMENT AND VALIDATION OF RP-LC METHOD

FOR ARIPIPRAZOLE IN PHARMACEUTICAL FORMULATIONS
Dr. D. Ravi Kumar1, Dr. V. Siva Ramakrishna2, T.Benjamin3,
T. Naga Raju1 and Dr. D. Ramachandran2
1
Department of Chemistry, Krishna University- Dr.MRAR PG Centre,
Nuzvid-521201, Andhra Pradesh, India.
3
Department of Chemistry, Noble College, Machilipatnam, India.
2
Department of Chemistry, Acharya Nagarjuna University, N.Nagar-522510.
Email: drkumar.orgchem@gmail.com
A new, simple, rapid, selective, precise and accurate isocratic reverse phase high
performance liquid Chromatography assay method has been developed for estimation of
Aripiprazole in tablet formulations. The separation was achieved by using column symmetry
C18(100 × 2.1 mm), 5µm column, in mobile phase consisted of Methanol and Water in the
ratio of 70:30 v/v. The flow rate was 0.8 mL/min-1 and the separated Aripiprazole was
detected using UV detector at the wavelength of 273 nm. Column temperature 25°C and
sample temperature ambient and injection volume 20µl. The retention time of Aripiprazole
was 2.29 min, indicative of rather shorter analysis time. The method was validated as per ICH
guidelines. The Linearity of Aripiprazole was carried out at different concentrations ranging
from 50-90ppm and correlation coefficient was found to be 0.999. Precision of the method
was performed by injecting standard injections, the %RSD value of Aripiprazole was found
to be 0.1%. Accuracy was confirmed by recovery studies. The percentage recovery of
Aripiprazole was found to be 100.07%.
(RACPABS - 2020)
PP-26: DEVELOPMENT AND VALIDATION OF SIMULTANEOUS

ESTIMATION OF GLYCOPYRROLATE AND FORMOTEROL
FUMARATE IN IT’S BULK AND PHARMACEUTICAL ROTA CAPS
DOSAGE FORM BY USING RP-UPLC
T.Benjamin1* , G.Rahul 2, Dr.Ch.Rajyalakshmi3 and Dr. D. Ramachandran4.

1
Department of Chemistry, Noble College, Machilipatnam, Andhra Pradesh, India
2
Department of Chemistry, The Hindu College, Machilipatnam, Andhra Pradesh, India
3
Department of Chemistry ,Vishnu Institute of Technology, Bhimavaram , Andhra Pradesh, India
4
Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur.
A new, simple, rapid, selective, precise and accurate isocratic reverse phase Ultra
Performance Liquid Chromatography (UPLC) assay method has been developed for
estimation of Glycopyrrolate and Formoterol fumarate in tablet formulations. The separation
was achieved by using column BEH C18 (50 x2.6mm, 1.8m) in mobile phase consisted of
Triethyl amine pH 2.5: Methanol in the ratio of (50:50 v/v). The flow rate was 0.2 mL/min
and the separated Glycopyrrolate and Formoterol fumarate was detected using UV detector at
the wavelength of 250 nm. Column oven temperature and sample cooler temperature set as
ambient. The retention time of Glycopyrrolate and Formoterol fumarate, was noted to be 0.68
and 1.04 minutes respectively, indicative of rather shorter analysis time 6.0 minutes. The
linearity range was found to be 22.5-135 µg/ml of Glycopyrrolate and 12-300µg/ml of
Formoterol fumarate and correlation coefficient of 0.999 and 0.999. Recovery was found to
be 99.74% and 99.90% for Glycopyrrolate and Formoterol fumarate. The %RSD of Precision
0.6 and 0.8 for Glycopyrrolate and Formoterol fumarate. The LOD and LOQ for
Glycopyrrolate was found to be 2.0 and 6.1 and LOD and LOQ for Formoterol fumarate was
found to be 1.3 and 3.9. The method was validated as per ICH guidelines. The proposed
method was found to be accurate, reproducible, and consistent.
(RACPABS - 2020)
PP-27: STUDY OF MOLECULAR & THERMODYNAMIC

PARAMETERS OF BINARY LIQUID MIXTURES BY USING
ULTRASONICS
* D. Ramachandran1, D.Nagendra2
*1
Department of Chemistry, ANU campus, Guntur
2
Department of Chemistry, RGUKT(APIIIT), Nuzvid.
1
dittakavirc@gmail.com ,2 nagendra.devanaboyina@rguktn.ac.in
The intermolecular forces of liquids in a mixture show a considerable effect on its

physical and chemical properties. There is continuing need for reliable thermodynamic data
of binary systems for chemical industries as the data is essential in the design of process
involving chemical separation, heat transfer, mass transfer and fluid flow. The ultrasonic
studies in liquid mixtures can be helpful in assessing degree of interaction between
molecules. The measurements of ultrasonic velocity (U), viscosity (η) and density (ρ) at
various temperatures (303K, 308K, 313K) over entire composition range and its data has
been used to calculate various thermodynamic and acoustic parameters like molar volume
(V), internal pressure ( ), adiabatic compressibility( ad), inter molecular free length (Lf),
acoustic impedance(Z), Enthalpy(H) and Gibbs free energy(G) etc. These results have been
analyzed and interpreted in terms of molecular dipole-dipole interactions. The data obtained
is used to understand intermolecular interactions between the unlike molecules and to test the
theories of solutions. The departure of these real mixtures from ideal behavior can be
explained in terms of effect of hydrogen bond breaking, loss of dipolar association,
differences in size and shapes, dipole-dipole interactions and complex forming tendencies of
different component molecules.
PP-28: DEVELOPMENT AND VALIDATION OF A NEW RP – HPLC

METHOD FOR ESTIMATION OF DAPAGLIFLOZIN
Dr. P. Srinivasa Rao, Head of the Dept. of Chemistry, SVRM College- Nagaram
Mr. R. Prasanna Babu, Lect. in Chemistry, SVRM College- Nagaram
A simple, rapid, precise, sensitive and reproducible reverse phase high performance
liquid chromatography (RP-HPLC) method has been developed for the quantitative analysis
of Dapagliflozin in pharmaceutical dosage form. Chromatographic separation of
Dapagliflozin was achieved on Waters Alliance -2695, by using Luna C18 (250mm x 4.6mm,
5µm) column and the mobile phase containing 0.1% TEA adjust pH-2.5 with OPA & ACN
(RACPABS - 2020)
in the ratio of 60:40% v/v. The flow rate was 1.0 ml/min, detection was carried out by
absorption at 296 nm using a photodiode array detector at ambient temperature. The number
of theoretical plates and tailing factor for Dapagliflozin were NLT 2000 and should not more
than 2 respectively. The linearity of the method was excellent over the concentration range
10-120 µg/ml for Dapagliflozin respectively. The correlation coefficient was 0.999. %
Relative standard deviation of peak areas of all measurements always less than 2.0. The
proposed method was validated according to ICH guidelines. The method was found to be
simple, economical, suitable, precise, accurate & robust method for quantitative analysis of
Dapagliflozin and study of its stability.
PP-29: SIMULTANEOUS DETERMINATION OF DICLOFENAC

SODIUM AND TOLPERISONE HCL IN MARKETED TABLETS BY
RP-HPLC METHOD
T.Naga Raju1, D.Ravi Kumar1, S.V.M.Vardhan2 and D.Ramachandran*

1
Department of Chemistry,Krishna University-Dr.MRAR PG Centre,Nuzvid-521201.
2
Department of Bio Chemistry,Krishna University-Dr.MRAR PG Centre,Nuzvid-521201.
*Department of Chemistry,Acharya Nagarjuna University, N.Nagar-522510.
Email:nagarajutalam@gmail.com
A novel, precise and accurate validated RP- HPLC method for the simultaneous assay
of diclofenac sodium[1] and tolperisone HCl[2] in combined dosage formulations was
developed. Existing literature reveals that few methods were reported for the simultaneous
assay of selected drugs[3-4]. The chromatographic separation of diclofenac sodium and
tolperisone HCl were performed using Hypersil column (I.D; 5µ, 250 x 4.6mm), maintained
at ambient temperature, and at the flow rate of 1.0 mL/min. The mobile phase consisted of
methanol, acetonitrile and 0.1% Triethylamine buffer (pH-4.5) in the ratio of 60:30:10
%v/v/v was used. Measurements were made with injection volume 20μL and ultraviolet
detection at 232nm. The total run time was 10.0minutes and the retention times of diclofenac
sodium and tolperisone HCl were 2.246 and 6.342 minutes respectively. The developed
method was validated as per ICH guidelines. Linearity for diclofenac sodium and tolperisone
HCl were found in the range of 10 - 30μg/mL and 50 - 150 μg/mL, respectively. The % drug
content of diclofenac sodium and tolperisone HCl in market tablets was found to be 99.98
and 99.99% respectively, making the developed RP-HPLC method to have potential
application in pharmacokinetic studies for diclofenac sodium and tolperisone HCl
combinations respectively.
(RACPABS - 2020)
PP-30: SELECTIVE SEPARATION, DETECTION OF DAUNORUBICIN

AND CYTARABINE MASS SPECTRAL CHARACTERIZATION OF
DEGRADANTS BY LC-MS/MS/QTOF

Department of chemistry
Acharya Nagarjuna University, Nagarjuna nagar- 522510
Two simple and sensitive spectrophotometric and liquid chromatographic (LC)

methods are described for the determination of Daunorubicin and Cytarabine. The first
method was based on the absorption factor. Daunorubicin and Cytarabine exhibit λmax 280nm.
Beer’s law was obeyed for Daunorubicin and Cytarabine at 4.4-88µg/ml and 10-200µg/ml
respectively. The second method, high-performance liquid chromatographic method was
developed for the determination of Daunorubicin and Cytarabine using Acetonitrile and 0.1%
Tri ethyl amine of pH-2.5 adjusted with OPA (20:80 v/v) as the mobile phase. The analysis
was performed on a X-Bridge C18 (150x4.6mm, 3.5µ) column. The calibration curve was
obtained for Daunorubicin and Cytarabine at 4.4-88µg/ml and 10-200µg/ml respectively. The
mean recovery was 99.9 and 98.7 respectively. The methods were validated according to the
ICH guidelines.
PP-31: THERMODYNAMIC PARAMETERS OF BINARY LIQUID

MIXTURES OF CHLOROFORM AND O- SUBSTITUTED ANILINE
K. Naveen kumara, K. Sambasiva Raob

a
Department of Physics, Acharya Nagarjuna University, Guntur-522510, AP, India
b
Department of Physics, J.K.C.College, Acharya Nagarjuna University, Guntur-522006, AP,
India; *Corresponding Author e-mail: ksrjkc@rediff.com
The densities (ρ) speeds of sound (u)and viscosities () are reported for binary
mixtures of chloroform with ortho-substituted aniline (2-chloroaniline, 2-methylaniline and
2-methoxyaniline) over the entire composition range from 303.15 K to 318.15 K and at
atmospheric pressure 0.1 MPa. The excess / deviation functions (VE, κsE and ) are
calculated from the measured densities, speeds of sound and viscosities at experimental
temperatures. The excess properties have been analysed through molecular interactions. The
FT-IR data indicate that the formation of intermolecular hydrogen bonding (N….H-C)
between nitrogen atom of amine group of o-substituted aniline and hydrogen atom of
chloroform in the binary liquid mixtures.
(RACPABS - 2020)
PP-32: INFLUENCE ON CHLORINE ATOM ON THERMODYNAMIC

AND TRANSPORT PROPERTIES OF BINARY LIQUID MIXTURES
AT VARIOUS TEMPERATURES (HALO-HYDROCARBONS AND 3-
METHOXYANILINE)
P. Nagababua, M. Gowrisankarb*
a
Department of Chemistry, Acharya Nagarjuna University, Guntur-522510, AP,
India. Email nagababuchem@rediff.com
b
Department of Chemistry, J.K.C.C. Acharya Nagarjuna University, Guntur-522006, AP,
India; Contact email: gowrisankar127@gmail.com
The densities (ρ), ultrasonic speeds (u), and viscosities (η) are reported for binary
mixtures of o-chlorotoluene, m-chlorotoluene and p-chlorotoluene with 3-methoxy aniline at
temperature range from (303.15 to 318.15) K and at a pressure p = 0.1 MPa. In addition,
excess functions such as excess molar volume, excess isentropic compressibility and
deviation in viscosity are calculated from the density, speed of sound and viscosity and the
°E
Redlich – Kister equation, correlates these properties. The excess partial molar volumes, Vm,1
°E E °E
and Vm,2 , and excess partial molar isentropic compressibilities, Ks,m,1 and K s,m,2 of the
components at infinite dilution are calculated. As well, the calculated excess properties were
discussed in terms of influence of chlorine atom on interactions between halo hydrocarbons
and 3-methoxy aniline. Moreover, the VE values have been analyzed with Prigogine- Flory-
Patterson theory and shown that the free volume contribution is the supporting factor for
negative values of excess molar quantities
PP-33: CONVERGENT SYNTHESIS OF ALIPHATIC AND AROMATIC

THIOUREAS
Venkata Bhavanarushi Pendema, Nannapaneni Madhavi*b

a
Department of Chemistry, RGUKT Srikakulam, ,A.P.
b
PG Department of Chemistry, J.K.C College, Guntur, Andhra Pradesh, India
E-Mail: rushichem09@gmail.com
In a one pot strategy we have achieved an efficient method for the synthesis of
alkyl/aryl thioureas starting from amines. In this strategy thioureas were prepared by
desulfurization of thiocarbomate salt which was formed by reaction of amines with
carbondisulphide. All the reactions were carried out under mild reaction conditions by using
eco-friendly solvents and reagents and the target products were prepared in excellent yields.
(RACPABS - 2020)
Scheme:
PP-34: BINARY MIXTURES OF 2,6-DIMETHYL CYCLOHEXANONE

WITH VARIOUS FUNCTIONAL GROUPS (M-CRESOL, P-CRESOL
AND ORTHO-CHLOROPHENOL)
Shaik Parveen Sulthanaa, M. Gowrisankarb*

a
Department of Chemistry, Acharya Nagarjuna University, Guntur-522510, AP,
India. Email : mail2sulthana@gmail.com
b
India; Corresponding author Email: gowrisankar127@gmail.com
In the binary mixtures of 2,6-dimethyl cyclohexanone with various functional groups

(m-cresol, p-cresol and orthochlorophenol) the densities, speeds of sound and viscosities
including those of pure liquids, were measured over the entire composition range at different
temperatures (303.15 -, 318.15) K and atmospheric pressure 0.1MPa. Using this
experimentally determined data, the excess values of molar volume, deviation in isentropic
compressibility, partial molar volumes, partial molar isentropic compressibilities and
deviation in viscosity of these components at infinite dilution were calculated. The results are
discussed both in terms acid-base interactions and the formation of - complexes between
the unlike molecules.
(RACPABS - 2020)
PP-35: THERMODYNAMIC PARAMETERS OF BINARY LIQUID

MIXTURES OF ORTHO-NITRO TOLUENE WITH VARIOUS
FUNCTIONAL GROUPS (2-ETHYL-1-HEXANOL, 3-
METHYLANILINE, AND 2,6-DIMETHYLCYCLOHEXANONE)
D.Rahula, D. Ramachandranb,*,
a
Department of Chemistry, J.K.C.C. Acharya Nagarjuna University, Guntur-522006,
b
Department of Chemistry, Acharya Nagarjuna University, Guntur-522510, AP, India,
*Corresponding Author e-mail: dittakavirc@gmail.com
In the binary mixtures of ortho-nitro toluene with various functional groups (2-ethyl-
1-hexanol, 3-methyl aniline, and 2,6-dimethyl cyclohexanone) densities, speeds of sound and
viscosities including those of pure liquids were measured over the entire composition range at
different temperatures (303.15, 308.15 and 313.15) K and atmospheric pressure 0.1MPa.
Using this experimentally determined data, the excess  deviation parameters (molar volume,
isentropic compressibility and deviation in viscosity) partial molar volumes, partial molar
isentropic compressibilities of these components at infinite dilution were calculated. The
results are discussed in terms the intermolecular forces between the different component
molecules in the binary mixture are more than the average intermolecular forces existing
between the similar molecules of pure components.
PP-36: MOLECULAR INTERACTIONS IN BINARY MIXTURES

CONTAINING HALO-SUBSTITUTED CARBONYL COMPOUND+
ALKOXY ALKANOLSAT VARIOUS TEMPERATURES
M.RadhaSirija, P.Bharath,D.Ramachandran*
Email: sireeja.maganti@gmail.com,dittakavirc@gmail.com
Thermo-physical, and excess parameters has a great impact to know the behaviour
and type of molecular interactions exist between the components of binary liquid mixtures at
different mole fractions and temperatures. Densities, viscosities and ultrasonic velocities of
binary liquid mixtures of halo-substituted carbonyl compounds with Alkoxy alkanols have
been measured over the entire composition range and at temperatures
303.15K,308.15K,313.15K and 318.15K. From experimental data, Excessparameters were
calculated. The obtained values were fitted to the Redlich-Kister polynomial equation. The
parameters of excess functions are found sensitivefor the intermolecular interactions between
the binary mixtures. Theoretical values of viscosity of the binary mixtures were calculated
using empirical relations and theoretical equations.The relative merits of these relations and
theories were discussed.
(RACPABS - 2020)
PP-37: PHARMACOLOGICAL STUDIES OF COBALT OXIDE

NANOPARTICLES
VeronicaDeekala, Jyothsna Pragathi Yazala1, Anitha Kowthalam2,

,RameshrajuRudraraju*1
1.Departmennt Of Chemistry, Acharya Nagarjuna University, Nagarjuna nagar-522510
2.Department Of Chemistry, Sri Krishnadevaraya University,Ananthapur-515003.
The cobalt oxide Nanoparticles were synthesized from Cobalt Nitrate Hexahydrate
aqueous solution under the chemical method at 60-90°C. FESEM, EDX, XRD were used to
characterize the structural features of the product. FTIR spectra confirmed the adsorption of
the cobalt oxide nanoparticles. In addition, UV-visible absorption spectra were employed to
estimate the band gap energy of the copper oxide nanoparticles. The effect of cobalt oxide
nanoparticles is screened in vitro for antimicrobial activity by Disc diffusion method. The
bacterial organisms used in this study are E.coli, Bascillus Subtilis. The observed inhibition
zones for these nanoparticles are in the range of 22mm for E.coli and 20mm for Bascillus
Subtilis. The cytotoxicity activities of copper oxide nanoparticles screened by MTT-assay.
We have screened for one type of cancer cell-line i.e MCF-7( Breast Cancer), cobalt oxide
nanoparticles obtained IC50 values in the range of 54ug/ml for MCF-7 cell line.
PP-38: SYNTHESIS AND CHARACTERIZATION OF

SILVERNANOPARTICLES USING LEAF EXTRACT OF THE PLANT
ALTERNANTHERABRASILIANA
KollimarlaSiri, G. Sravani, K. PreeniPraneethaand K. PrasadaRao*

*email ID: prasad17467@gmail.com
Due to the wide range of applications and increased properties, there has been an
increased emphasis on the topic of green synthesis of silver nanoparticles (AgNps).
Nanoparticles which are having one structural dimension of less than 100nm, making them
comparable in size to subcellular structures, including cell organelles or biological
macromolecules and enabling their ready incorporation into biological systems[1].
Alternantherabrasiliana is an erect, herbaceous plant that can grow up to 3 metres tall, but is
usually less than 1 metre in cultivation[2].The plant is sometimes harvested from the wild for
local use as a food and medicine. It is often grown as an ornamental. It is evergreen, perennial
herbs, native totropical and sub-tropical regions of Brazil, Australia & South America.. All of
(RACPABS - 2020)
its parts are used in traditional system of medicine; leaves arethe most important parts which
are use medicinally.Traditionally, the plant is used as a galactagogue, cholagogue,
abortifacient and febrifuge. It is also used for indigestion. The leaves are used like spinach,
and in soups. It is claimed to be a good fodder which increases milk in cattle. It is also used
for hazy vision, night blindness, diarrhoea, dysentery and post-natal complaints. The
phytochemistry of the extract of the leaves has been studied. The green synthesis of silver
nanoparticles was done using aqueous leaf extract of Alternantherabrasiliana has been
carried out and synthesised silver nanoparticles (AgNPs) were characterized with UV-Vis-
spectroscopy, Fourier transform infrared (FTIR) analysis and Scanning Electron Microscope
(SEM) Diffraction and Powder X-Ray Diffraction [3].The prepared AgNPs are found to be
mostly spherical shapes.SEM analysis was carried out to understand the topology and the size
of the Ag-NPs. The activity of the synthesised nanoparticles is studied and the reports show
the presence of significant antimicrobial activity.
PP-39: THERMO PHYSICAL AND SPECTROSCOPIC PROPERTIES

OF BINARY LIQUID SYSTEMS OF 2-METHYLPROPONIC ACID
WITH VARIOUS FUNCTIONAL GROUPS (2-ETHYL-1-HEXANOL, 2-
METHYLANILINE, 2-METHOXYANILINE AND 2-
METHYLCYCLOHEXANONE)
P. VenkateswaraRaoa, M. Gowrisankarb, K. Ravindhranathc*

a
Department of Chemistry, R.V.R. & J.C. Acharya Nagarjuna University, Guntur-522019, A.P, India
b
Department of Chemistry, J.K.C.C. Acharya Nagarjuna University, Guntur-522006, AP, India
c
Department of Chemistry, K L University, Guntur-522502, AP, India
*Corresponding Author e-mail: mgsankar965@gmail.com
In the binary mixtures of 2-methylproponic acid with various functional groups (2-
ethyl-1-hexanol, 2-methyl aniline, 2-methoxy aniline and 2-methyl cyclohexanone) densities,
speeds of sound and viscosities including those of pure liquids were measured over the entire
pressure 0.1MPa. Using this experimentally determined data, the excess  deviation
parameters (molar volume, isentropic compressibility and deviation in viscosity) partial
molar volumes, partial molar isentropic compressibilities of these components at infinite
dilution were calculated. The results are discussed both in terms of formation of hydrogen
bonds in the amine and the formation of - complexes between the unlike molecules. The
results are analyzed in terms of dipole-dipole interactions and hydrogen bonding between 2-
methylproponic acid and various functional groups. The VE results are analyzed in the light of
Prigogine-Flory-Patterson theory. Analysis of each of the three contributions viz.
(RACPABS - 2020)
interactional, free volume and P* to VE has shown that interactional contribution are positive
for all systems while the free volume and P* contributions are negative for all the binary
mixtures. The variations of these parameters with composition and temperature are discussed
in terms of intermolecular interactions prevailing in these mixtures. A good agreement is
obtained between excess quantities and spectroscopic data.
PP-40: ESTIMATION OF STRENGTH OF MOLECULAR

INTERACTIONS BETWEEN 2,6-DIMETHYLCYCLOHEXANONE AND
CHLORINATED ETHANE / ETHENE COMPONENTS AT VARIOUS
TEMPERATURES
K. Srinivasa Raoa, M. Gowrisankarb*

a
Department of Chemistry, Acharya Nagarjuna University, Guntur-522510, AP, India
b
Data on density, speed of sound, and viscosity for mixtures of 2,6-

dimethylcyclohexanone with 1,2-dichloroethane, 1,1,1-trichloroethane,1,1,2,2-
tetrachloroethane, trichloroethene, and tetra chloroethene over the entire composition range
of mole fraction at T = (303.15 - 313.15) K and at atmospheric pressure 0.1 MPa. The results
are used to excess molar volume, excess isentropic compressibility and deviations in
viscosity. Values of excess isentropic compressibility are positive in mixtures of 2,6-
dimethylcyclohexanone with 1,2dichloroethane and tetrachloroethene and negative in the
systems 2,6-dimethylcyclohexanone + 1,1,1-trichloroethane, +1,1,2,2-tetrachloroethane, and
+ trichloroethene. All the systems exhibit positive deviation in viscosity. The results are
interpreted in terms of electron-donor-acceptor interactions between unlike molecules.
(RACPABS - 2020)
PP-41: LIQUID CHROMATOGRAPHIC ESTIMATION OF

MIANSERIN
Giri Prasad Gorumutchu1and Venkata Nadh Ratnakaram 2*

1
Department of Chemistry, SBG Degree and PG College, Satenapalli-522510, India;
2
GITAM University – Bengaluru, Karnataka-562163, India.
Email: doctornadh@yahoo.co.in; Mobile: +91-99902632733
Mianserin is a popular medicine for depression and exhibits activities of

antihistaminic and hypnosedative. It causes a stimulateddischarge of norepinephrine. A
reverse phase high performance liquid chromatographic method was developed for the
estimation of mianserin. The separation conditions (chromatographic column selection,
composition and pH of eluting agent, flow rate) were optimized. The established procedure
was validated as per the current ICH guidelines. The developed method was validated with
respect to sensitivity, linearity, precision, accuracy and robustness.
PP-42: ULTRA PERFORMANANCE REVERSE PHASE LIQUID

CHROMATOGRAPHIC METHOD FOR THE QUANTIFICATION OF
2,4-DIFLUOROBENZOIC ACID IN ELVITEGRAVIR IN DRUG
PRODUCT
Dr.D.Mogilireddy
Fortune pharma training institute,Near JNTU, HYDERABAD-500049
8919286318;mogilireddy@gmail.com
A new and simple, rapid, selective gradient liquid chromatographic method was
developed for the quantification of 2,4-Difluorobenzoic acid in Elvitegravir. Good resolution
was achieved between 2,4-Difluorobenzoic acid in Elvitegravir and its related substances by
using Phenomenex Kinetex F5 100*2.1,1.7um column over a gradient elution comprising
mobile phase A (pH -3.0 buffer) and mobile phase B (Mixture of Acetonitrile: water:90:10
v\v ) at 35°C column oven temperature and Sample compartment temperature 5°C. The USP
Tailing factor for 2,4-Difluorobenzoic acid in Elvitegravir was found less than 1.5 and
Column efficiency Plate counts (N) was found 3571. Mobile phase flow was fixed at a rate of
0.3 mL/min and elution was monitored at 240 nm. The method was validated as per ICH
guidelines for accuracy, precision, specificity, linearity, sensitivity and stress conditions.
Linearity was observed for 2,4-Difluorobenzoic acid in Elvitegravir in the concentration
range of 0.05% to 0.15% obtained correlation coefficient was 0.9998. Obtained accuracy data
(RACPABS - 2020)
was from 99.6 to 100.2 from the range of 50% to 150% of the sample concentration. The
RSD for intra-day and inter-day precision was found to be less than 0.5% and the sample
solution was stable up to 48 hrs at 25°C. Validation studies demonstrated that this HPLC
method is stability-indicating, accurate, specific, rapid, reliable and reproducible.
PP-43: TOTAL SYNTHESIS OF DIPLODIALIDE C AND D
V.B.Ramanujan,Dr.Ch.N.Sai Pavan Kumar Dept of Chemistry, VFSTR University, Guntur
Diplodialides (C (1), and D (1a)) are the first 10-membered lactone pentaketides (Fig.
1), which were isolated from the plant pathogenic fungus Diplodiapinea (IFO 6472) by Wada
and Ishida4,5. Diplodialides exhibit various biological activities, in particular, inhibitory
activity against progesterone 11a-hydroxylase in vegetable cell cultures of Rhizopus
stolonifer at 125 ppm. The absolute stereochemistry of diplodialide C and D is (3R, 9R)
determined by Wada and Ishida6.
O O
O (R) O (R)
(R) (R)
HO HO
O
Diplodialide C (1) Diplodialide D (1a)
Figure 1
We report our successful total synthesis of compound 1 and 2 using Sequential double
alkylation of 1, 3-dithiane with bromide and chiral epoxide and Yamaguchi
macrolactonisation as the key steps. Our retro synthetic approach for the synthesis of 1 and
1a was outlined in Scheme 1. The target molecule 1 and 1a could be made from common
intermediate hydroxy acid 2 by intra molecular Yamaguchi macro-lactonization, whereas 2
could be synthesized from the coupling reaction of two key fragments bromo compound 4
and chiral epoxide 5 with 1,3 di thiane, while Both bromo compound 4 and chiral epoxide 5
could be obtained from known chiral epoxide.
O
O (R)
( R)
HO
O
1
OTBS S S
OH S S OPMB 3 O
Br
O (R) ( S)
COOH +
4 5
2
O (R)
( R)
HO
1a O
TsO
6
(RACPABS - 2020)
PP-44: ISOLATION AND CHARACTERIZATION OF NEW

PENTACYCLIC TRITERPENOID FROM CARALLUMA ATTENUATA
ROOT
G.Jayalakshmi, Prof. V. Anuradha*

Department of Chemistry,Vignan degree and PG College, Palakaluru road, Guntur.
Email: gjayalakshmi17@gmail.com
Recently triterpenoids are known to exhibit several pharmacological activities. These

compounds can be used as anticancer agents [1-4], anti HIV, antiinflammatory, antiviral and
against neurodegeneration disorders . Caralluma species known for many triterpenoids is one
among the best source in indian traditional medicine. From the roots of Caralluma attenuata
belonging to the family Asclepiadiacea , a novel pentacyclic triterpenoid, attendiol was
isolated using various chromatographic techniques. Its structure elucidated on the basis of
spectroscopic data. This is the first report of such novel pentacyclic triterpenoid from
Caralluma attenuata root. Isolation and structure elucidation will be discussed.
PP-45: PHARMACOLOGICAL STUDY OF ANTIDEPRESSANT ACTIVITY OF

PLOCAMA PENDULA ROOT EXTRACT
Dr. K. Uma Maheswar1*, Dr. KRS Prasad2, Chaitanya Darapureddy2

1. Dept. of Chemistry, Dr. APJ Abdul Kalam Campus, RGUKT, Ongole, AP, India
2. Department of Chemistry, K.L. University, Vaddeswaram-522 502, India
Plocama is a flowering plant genus belonging to Rubiaceae family. The natural

products are soxlated from the dried powder of Plocama root with various solvents like n-
Hexane, Ethyl acetate, Chloroform, Water and Ethanol. Phytochemical screening revealed
that Alkaloids are present in chloroform extract where as Anthocyanins are present in ethanol
extract. Terpenoids, Flavonoids, Alkaloids and Phenolic compounds are present in water
extract.In the present study Antidepressant activity of Plocama pendula root extract was
identified by forced swimming test(1) and Tail Suspension Test(2). Water Extract of
Plocama pendula root powder (PPRWE) (100, 200 and 400 mg/kg) administered to mice,
(RACPABS - 2020)
produced significant antidepressant‐like effect in both Tail Suspension Test and Forced swim
test and their efficacies were found to be comparable to Imipramine (15 mg/kg) and
Fluoxetine (20 mg/kg). Drugs that alter general motor activity(3) may give
false‐positive/negative results in the forced swimming test.The effects produced by PPRWE
and Diazepam (1.0mg/kg) upon the open field test demonstrated that these products do not
modify the spontaneous locomotor activity of mice, which indicates that the plant extract
exerts antidepressant effects without modifying significantly this parameter. Therefore, it is
probable that these effects are not related to the stimulation of general motor activity. Results
of this study showed that the administration of the PPRWE produced a diminution of
immobility time of mice(4) exposed to the both forced swimming and tail suspension tests.
Ecliptin alkaloid & Culumbin, a flavonoid present in PPRWE may be facilitating
monoaminergic transmission there by producing antidepressant effects. Harmaline alkaloids
present in Plocama pendula act as reversible monoamine oxidase inhibitors and in common
with other beta carboline binds to 5‐Hydroxy Tryptamine receptors. Inhibition of this enzyme
causes a reduction in metabolism and subsequent increase in the concentration of biogenic
amines. The flavonoid components of PPRWE might be interacting with adrenergic and
serotonergic systems in mediating the antidepressant effects of PPRWE. However, the
precise mechanisms by which the extract produced antidepressant‐like effect are not
completely understood. Further studies would be necessary to evaluate the contribution of
active chemical constituents for the observed antidepressant activity.
PP-46: SYNTHESIS OF 1,4-DIHYDROPYRIDINES DERIVATIVES
Upendar Reddy Mandadapu1, Murali Krishna Cheepi2 & Mahesh Madala3

1. Department of Chemistry Rayalaseema University,Kurnool 2.Department of Chemistry,
Adikavi Nannaya University, Rajamahendravaram 3.True Labs Private Limited, Hyderabad.
1,4-dihydropyridines (1,4-DHPs) represent a class of compounds possessing various

structural modified derivatives, particularly amongst natural products and bioactive agents.
The clinically important drugs such as Nicardipine, Nifedipine, Nimodipine, Felodipine,
Isradipine and Amlodipine appeared on the market with new active functional group in their
main skeleton. In recent years, the clinical use of the existing antimicrobials has been limited
due to the following parameters (i) relatively high risk of toxicity (ii) pharmacokinetic
problems and (iii) development of bacterial and fungal resistance resulting from the
widespread use and misuse of classical antimicrobial agents.
(RACPABS - 2020)
Retro synthetic analysis of 1,4-dihydropyridine derivatives (Scheme 1) revealed that

one pot synthesis of multi components (2-amino Chromone,benzaldehyde and substituted
1,3-diketo cyclohexane) in the presence of (±) lactic acid, ethyl-L-lactate.
Antibacterial evaluation: The synthesized 1,4-dihydropyridine derivatives were tested

against (a) Staphylococcus aureus (MTCC 902), (b) Bacillus subtilus (MTCC 441) a Gram
positive strains and (c) Escherichia coli (MTCC 2692), (d) Pseudomonas aeruginosa (MTCC
2453) a Gram negative strains (at concentration of 30 μg/mL) using agar well diffusion
method6-8. The results of the antibacterial activity of 1,4-dihydropyridine derivatives
indicated that compounds Methyl, Ethyl and Dimethyl exhibited good activities.
PP-47: SYNTHESIS THEORETICAL CALCULATION, DOCKING

STUDIES AND ANTI MICROBIAL EVALUATION OF 1-(3-FLUORO-4-
MORPHOLINOPHENYL)-1H-TETRAZOL-5-AMINE DERIVATIVES.
B.S.N Murthy1*, T. Ramana2, M. D. A. Saibabu3

1
Department of Chemistry, Sir C R Reddy P G college, Eluru-534007, A.P., India-534007.
2
Dept. Of Chemstry, GITAM Deemed to be University, Bengaluru Campus, Karnataka, India-562163
3
School of Chemistry, University of Hyderabad, Hyderabad, Telangana-500046.
*Corresponding author information: Dr. B. S N Murthy, E-mail: snmboddapati@gmail.com .
Tetrazoles are well known for their biological and pharmacological applications.
Inspired from these and in continuation of our efforts towards the development of various
biologically potent heterocycles, in this work the authors described their efforts towards the
(RACPABS - 2020)
development of a simple and effective method for the construction of novel N-substituted
acetyl and aryl-N-(1-(3-fluoro-4-morpholinophenyl)-1H-tetrazol-5-yl) amide derivatives and
evaluation of their in vitro antibacterial properties. All the synthesized compounds were
screened for their anti-bacterial properties against the three medically relevant organisms like
Escherichia coli, Staphylococcus aureus, Bacillus subtilis. Among the tested compounds the
compounds three compounds exhibited superior activity compared to reference drug.
Additionally the authors performed the HOMO-LUMO analysis and docking studies also.
For docking studies staphylococcus aureus “High resolution crystal structure of the Pleckstrin
Homology Domain of Protein Kinase B/Akt Bound To Ins(1,3,4,5)-Tetrakisphophate”
(1UNQ) retrieved from protein data bank was Protein Kinase B/Akt protein by using
Autodock 4.2. In the present investigation the authors mainly focused on the binding energy,
hydrogen bonds and distance between the protein and ligand. The results reveal that one of
the compound was potently bound to Lys20 with highest score ΔG = -6.09 Kcal/Mol and KI
= 34.51 µM.
PP-48: SYNTHESIS NI(II) COMPLEXES USING N, O DONOR SCHIFF

BASE LIGANDS: ANTIOXIDANT, ANTIMICROBIAL, DNA BINDING
AND CLEAVAGE STUDIES
V. Sumalathaa,, K.Geetha bhavania, K. Niramala Jyothia

a
Department of Chemistry, J.M.J College for Women, Tenali, Andhrapradesh-522202, India.
A series of two bivalent metal complexes M(L1)2 and M(L2)2 where M = Ni(II) and
HL1=2-((E)-(4-(trifluoromethoxy)phenylimino)methyl)-4-chlorophenol,HL2=2-((E)-(4-
(trifluoro methoxy) phenylimino) methyl)-4-nitrophenol were synthesized and characterized
by elemental analysis, SEM, Mass, 1H NMR, 13C NMR, UV–Vis, FT-IR, ESR and magnetic
susceptibility measurements. Based on the analytical and spectral data a square
planar geometry has been assigned to all the metal complexes. DNA binding
properties of these complexes have been explored using electronic absorption spectroscopy,
fluorescence spectroscopy and viscosity measurements. DNA cleavage efficacy of these
metal (II) complexes with super-coiled pBR322 DNA have been investigated by gel
electrophoresis in presence of H2O2 and UV light, and it is observed that all the metal
complexes showed better nuclease activity. The in vitro antimicrobial activity results by
paper disc method against few bacterial pathogens, fungal species have shown that the
complexes are biocidally active than free ligands. Finally the compounds were screened for
antioxidant activity using 1,1-diphenyl-2-picryl hydrazyl (DPPH) which was compared with
that of standard drug vitamin-C.
(RACPABS - 2020)
PP-49: A KINETIC STUDY ON THE OXIDATION OF POLYOLS BY N-

HALOAGENT
K.V.S.Koteswara Rao1 and R. Venkata Nadh2*

1
Department of Chemistry, GVSM Government Degree College, Ulavapadu-523292, India
2
* GITAM University – Bengaluru Campus, DoddaballapurTaluk, Bengaluru Rural District,
Karnataka - 561 203, India, email: doctornadh@yahoo.co.in; Mobile: +91-9902632733
Polyols are the approved sugars for consumption by human beings and are with low
calorie values. Ruthenium (III) catalysed kinetic study of the selected polyols (xylitol and
adonitol)was studied in acidic medium using DCICA, a familiar N-haloagent as an oxidant.
Reactions were unit order in oxidant concentration. Zero order reaction was observed in
substrate concentration. A decrease in the rate of reaction with an increase in acid
concentration was observed. Effect of temperature was studied. Mechanisms of oxidation
reactions were proposed based on the kinetic orders.
PP-50: EXTRACTIVE ION PAIR COMPLEX FORMATION FOR

DETERMINATION OF FEBUXOSTAT
Sudhir S Muvvala1,VenkataNadh Ratnakaram2*

1
Department of Chemistry, Sri Subbaraya and Narayana College, Narasaraopet – 522 601,
Andhra Pradesh, India, Tel: +91 9490645147, email: sudheer45147@yahoo.com
2
GITAM University – Bengaluru, Karnataka-562163, India;
A simple, sensitive and economical visible spectrophotometric method was proposed

for determination of febuxostat in bulk and pharmaceutical dosage forms. The proposed
method was based on the formation of an ion-pair complex between Febuxostat (drug) and a
basic dye Methylene blue (0.2 % w/v) in presence of aammonia–ammonium chloride buffer
(pH 9.8) followed by extraction of the complex into chloroform, and measuring the
absorbance of blue green colored drug-dye complex at 620 nm. The linear regression
analysis data for the calibration plot showed good linear relationship (r = 0.99937) with in the
concentration range of 4 – 20 μg mL-1. The limit of detection and limit of quantitation were
found to be 0.150 μg mL-1and 0.454 μg mL-1.The proposed method was tested and validated
for various parameters according to ICH guidelines and the results demonstrated that the pro-
cedure is accurate, precise and reproducible (R.S.D. < 2 %).
(RACPABS - 2020)
PP-51: MICROWAVE ASSISTED SYNTHESIS AND ANTI-

INFLAMMATORY ACTIVITY EVALUATION OF PYRAZOLE
DERIVATIVES
P. Naveen Kumar B. Ch. Naga Raju, K. Anjaneyulu and Dr. Ch. Subramanyam*
Department of Chemistry, Bapatla Engineering College, Bapatla, India
Corresponding author: Email: *subbusree04@gmail.com, 9490385962
Pyrazole refer to a class of simple aromatic ring organic compound of heterocyclic

diazole series characterized by a 5- member ring structure composed of three carbon atom
and two nitrogen atom in the adjacent position and to unsaturated parent compound. These
are important organic compounds for pharmaceutical [1] and agrochemical industry [2].
Microwave promoted easy, efficient and environment friendly procedure has been devised for
the synthesis of a series of pyrazole derivatives in high yields by the reaction of chalcones
with phenyl hydrazine hydrochloride under catalyst free, solvent free conditions. The newly
synthesized compounds were characterized by NMR (1H and 13C), IR spectra. In vitro anti-
inflammatory activity of the synthesized compounds was investigated against inhibition of
Albumin Denaturation and Membrane Stabilization test methods. Majority of the compounds
showed good activity when compared with the standard drugs.
PP-52: HPLC METHOD DEVELOPMENT AND VALIDATION FOR

THE ESTIMATION OF SULFOMOXOLE IN BULK AND
FORMULATIONS
M.KamalaKaruna1andR.VenkataNadh2*
1
Department of Chemistry, Government Women’s College, Guntur-522001, India.
2
A rapid, sensitive, RP-HPLC method has been developed and validated for the
estimation of sulfomoxole in bulk and pharmaceutical formulations. In this method we used
C18 column (250x 4.6 mm) and a mobile phase methanol, acetonitrile and water in
55:30:159(w/v) at 5.8 PH, at a flow rate of 1ml/min at room temperature. Detection was
carried out using a UV detector at236nm. The total chromatographic time was 10 min. The
linearity is between the range 5-30 µgm/ml. The proposed method was validated according
to ICH guide lines. Forced degradation conditions of hydrolysis, oxidation, photolytic and
thermal stress were also performed.
(RACPABS - 2020)
PP-53: DESIGN AN EFFICIENT METHOD FOR THE SYNTHESIS OF

2-(1,3-DIPHENYL-1H-PYRAZOL-4-YL)BENZO[D]THIAZOLE AND
STUDY THEIR ANTIMICROBIAL ACTIVITY
B. Srinivas*1 and K. Saidulu2

1
Department of Humanities and Sciences, Guru Nanak Institutions Technical Campus,
Ibrahimpatam, Telangana
2
Department of Chemistry, Nagarjuna Government College, Nalgonda, Telangana
*srichemistry@gmail.com
Benzothiazoles are important heterocyclic compounds and it has diversified valuable

applications in the area of drug discovery and pharmaceutical chemistry [1,2] such as
including antimicrobial [3,4] and antifungal, cytotoxic,and antidiabetic applications and also
substituted benzo thiazole have been designed and synthesized for biological evaluations.
Pyrazoles are the privileged compounds for the pharmaceutical and agricultural research,
such as Celebrex, Viagra, Zometapine, Cyenopyrafen,Fenpyroximate and Tebufenpyrad and
pyrazole containing compounds in the field of medicinal chemistry such as anticancer, and
antimicrobial activities. A simple, highly efficient and environmentally friendly method has
been developed for the synthesis of 2-(1,3-diphenyl-1H-pyrazol-4-yl)benzo[d]thiazole by
using Gadolinium related sulfonatecatalyst and ethanol reflux reaction conditions by using
this method. Here synthesized pyrazolo benzo thiazole derivatives these are an evaluation for
their antimicrobial activity, some derivatives were showed excellent antimicrobial activity on
both Gram-positive and gram-negative bacterial strains.
PP-54: QUALITY CHARACTERISTICS OF SOME EDIBLE OILS AND

EFFECT OF ADDITIVES ON CHEMICAL CONSTANTS
B.Jyothi1, Sk.Ameena2, A.Amulya3, N Madhavi4 *.

The Present investigation deals with analysis of iodine ,saponification ,peroxide and
acid values of available samples in Guntur district of Andhra Pradesh. The least iodine value
was identified for coconut oil.Sunflower oil showed the highest saponification value. Lowest
acid values were noticed for rice bran and palm oil. By addition of carrot, papaya and lime
the variations of chemical constants of oil samples were analysed. Little variation is observed
in case of saponification values and noticeable changes were investigated for peroxide values.
Almost same values were resulted in case of iodine and acid values.
(RACPABS - 2020)
PP-55: BIOLOGICAL SCREENING OF PYRIMIDINES AND

PHENOTHIAZINES
Boggavarapu Jyothi1, Thiyyagura Sravanthi2 ,Suryadevara Kalpana3 ,

Nannapaneni Madhavi*
2,Department Of Chemistry,Sdmsm Kalasala,Vijayawada
1, 2 ,*Pg Department Of Chemistry, J.K.C College, Guntur, Andhra Pradesh, India
Corresponding Author Email : Madhavijkcchempg@gmail.com;9441021731
We evaluated the selected pyrimidines and phenothiazines for their antibacterial

activity by using the broth dilution nutrient agar method against gram negative bacteria. Their
antibacterial activity is aggrandized due to the omnipresence of chloro and fluoro groups in
the benzene ring of pyrimidine linked benzene sulphonamide. In phenothiazines their activity
is due to the latency of nitro, methyl and fluoro groups espied in phenyl ring of
phenothiazines.Antifungal activities were implacable by agar cup assay method. Due to the
bromo and fluoro groups on benzene ring of pyrimidine linked sulphonamides their
antifungal activity is emphasized. Methyl and fluoro groups on the benzene ring of
phenothiazines are obligated for antifungal activity.
PP-56: SYNTHESIS AND CHARACTERIZATION OF SILVER

NANOPARTICLES USING CAMPSIS GRANDIFOLIA PLANT
EXTRACT
B.Suresh, B. Bhaskar, E. Narsimha Reddy, P. Nagaraju and K. PrasadaRao*

Email id: *prasad17467@gmail.com
An eco-friendly green synthesis of silver nanoparticles using leaf extract of Campsis

grandifolia was investigated. Nanotechnology is a science based on atomic, molecular and
supramolecular molecules aiming to create nano-structures with enhanced functionalities [1],
and the term nanoparticle describes particulate matter ranging in size from 1–100 nm Bearing
a nano scale size offers the advantage of having a significantly large surface area to volume
ratio. Green synthesis of NPs using plants extract has several advantages over chemical
synthesis, such as simplicity, cost effectiveness as well as compatibility for biomedical and
pharmaceutical applications [2]. Silver nanoparticles have excellent medical and nonmedical
properties and applications when compared with other metal nanoparticles[3]. The main
advantage of using plant extracts to synthesize silver nanoparticles is easy accessibility, Safe
(RACPABS - 2020)
and in most cases non-toxic plants which can help to reduce silver ions. The flowers and the
whole plant of Campsis grandifolia are blood tonic, carminative, depurative diuretic and
febrifuge. They are used in the treatment of women's complaints. The plant extract has been
and is used to correct menstrual disorders, rheumatoid pains, traumatic injuries, difficult
urination, pruritis and oozing dermaphytoses. The silver nanoparticles of this plant leaf
extract haven prepared in aqueous medium. The silver Nanoparticles were charecterised by
UV Visible, FTIR, SEM analysis and XRD spectrum. The obtained particles have studied for
their biological activity and shown good antimicrobial activity.
PP-57: SYNTHESIS OF CHAIN TRANSFER AGENT FOR

REVERSIBLE ADDITION FRAGMENTATION CHAIN TRANSFER
(RAFT) POLYMERIZATION TECHNIQUE.
B.S.V.S.M.Ganesh1 , D Ramachandran
Email: dittakavirc@gmail.com
This paper provides an overview and discusses some recent developments in

reversible addition fragmentation Chain transfer (RAFT) polymerization .guidelines for the
selection of RAFT agents are presented .RAFT Polymerization with appropriately designed
trithiocarbonate RAFT agents is successfully applied the utility of raft process is then
illustrated with synthesis of polymers is then illustrated with synthesis of polymer with
reactive end group. And here we also report the synthesis and uses of versatile chain transfer
agent (CTA) that produce well controlled macromolecular architectures wit specific chain
end functionalities via using RAFT polymerization including block co polymer, star polymer,
linear polymer. And these CTAs are also used for the grafting of poly styrene and poly
methyl acrylate from cotton.IN this project we synthesis 4-cano 4(dodecyl
sulfanoylthiocarbonyl) sulfnyl pentanoic acid (CDP)a chain transfer agent (CTA).
(RACPABS - 2020)
PP-58: VALIDATED METHOD DEVELOPMENT FOR THE ASSAY OF

BREXPIPRAZOLE IN PHARMACEUTICAL FORMULATIONS
Dr T N V S S Satyadev
Dept of Chemistry, P B Siddhartha College of Arts & Science, Vijayawada - 10
sdtpati@gmail.com
Analytical method was developed for the estimation of Brexpiprazole drug substance
by liquid chromatography. The chromatographic separation was achieved on Inertsil ODS C8
150*4.6, 3um at ambient temperature. The separation achieved employing a mobile phase
consisting of 0.1%v/v Trifluoro acetic acid in water: Acetonitrile (30:70). The flow rate was
0.7 ml/ minute and ultra violet detector at 315nm. The average retention time for
Brexpiprazole was found to be 2.16 min. The proposed method was validated for selectivity,
precision, linearity and accuracy. All validation parameters were within the acceptable range.
The assay methods were found to be linear from 50-150µg/ml for Brexpiprazole.
PP-59: PREPARATION OF LIPBALM FROM THE PEEL OF

HYLOCEREUS UNDATUS
K. Aravind lokesh, P. Asha Bhanu, G. Swapna, and Prof.B. Kesava Rao,*

Department of Chemistry, University College of Sciences, Acharya Nagarjuna University,
Nagarjuna Nagar-522510, Guntur Dt, Andhra Pradesh, India
krbhattiprolu@gmail.com
Hylocereus undatus fruit belongs to the family (Cactaceae) and it is commonly known
as dragon fruit. This research was emphasized on the application of natural colorant extracted
from peels of Dragon Fruit in the preparation of lip balm. In this research, betalain colorant
was extracted from peels of dragon fruit using ethanol. The extraction parameters such as
extraction time, temperature and ratio of material to solvent were determined for the
maximum yield of betalain. The colorant was identified by Ultraviolet Spectroscopy. The
betalain colorant (0.2%) was used in the preparation of lip balm. Further the methanolic
extract of dragon fruit was subjected for saponification.
(RACPABS - 2020)
PP-60: SYNTHESIS AND CHARACTERIZATION OF SILVER

NANOPARTICLES USING POLYSTICHUM MUNITUM PLANT
EXTRACT
G. V. V. Ramesh, D. Lakshmi Narayana, P. Manikanta Reddy and K. PrasadaRao*

Email ID: *prasad17467@gmail.com
Silver is extensively used in nanosystems and employed in various biomedical

purposes. Silver nanoparticles have excellent medical and nonmedical properties and
applications when compared with other metal nanoparticles [1]. The green approach of
nanoparticles synthesis possesses reduced or no toxicity and number of plants and herbal
extracts has been reported to be involved in such synthesis. Plant extracts contain number of
secondary metabolite which plays a critical role during the nanoparticle synthesis by acting as
reducing or capping agents [2]. Studies have shown that silver nanoparticles are highly stable
and toxic to bacteria, fungus, and viruses. An infusion of the fronds has been used as a wash
or poultice to treat boils and sores. The young shoots have been chewed and eaten as a
treatment for cancer of the womb and to treat sore throats and tonsillitis. The leaves have
been chewed by women to facilitate childbirth. The leaf extract has antimicrobial activity and
we have used low cost, ecofriendly, and quick method for the synthesis of silver
nanoparticles using Polysticum munitum. These nanoparticles were characterised by their UV
Visible, FTIR, and SEM anasys.
PP-61: EXTRACTION AND SAPONIFICATION OF OIL FROM

AVOCADO FRUIT
M.V.S. Manikanta, P Asha Bhanu and G Swapna, Prof.B. kesava Rao,*

The avocado tree with probable origin in south-central Mexico. It is classified as a

member of the flowering plant family Lauraceae. Avocado oil has generated growing interest
among consumers due to its nutritional and technological characteristics, which is evidence
by an increase in the number of scientific articles that have been published on it. The purpose
of the present research was to discuss the extraction methods, chemical compositions and
saponification of avocado oil. Our research work carried out through a systematic literature
search in scientific database. The quality of avocado oil will depend on the quantity and
(RACPABS - 2020)
maturity of the fruit and extraction technique in relation to temperature, solvents and
conservation. While avocado fruit has been studied, there is a lack of knowledge about
avocado oil and the potential health effects of consuming it. On the basis of literature data,
avocado oil has been established itself as oil that has a very good nutritional value and health
benefits.
PP-62: PRELIMINARY CHEMICAL INVESTIGATION OF CLITORIA

TERNATEA
G. Chandrika , P. Asha Bhanu, G. Swapna, and Prof.B. Kesava Rao,*

Clitoria ternatea, commonly known as butterfly pea or blue pea, is found throughout
the tropical regions. The plant bears blue flowers and grows in wild and also in gardens. It is
well known as tropical perennial climber herb from family Fabaceae with slender downy
stem. The flower could be used as a natural colorant. Anthocyanins in the flower are the
major constituents, determines the color. Pelargonidin is responsible for orange-red color,
cyanidin for red colour and delphinidin for blue color. Anthocyanins are also widely used as a
natural supplements and have been shown to have antiinflammatory activity, anti-oxidant,
anticarcinogenic and anti -microbial activities.The chemical compounds isolated from clitoria
ternatea include various triterpenodis,flavonol, glycosides, anthocyanins and steroids. The
color of Clitoria is a result of anthocyanins
PP-63: PHYTOCHEMICAL INVESTIGATION OF CURCUMA LONGA
G. Kumudini Sri, P. Asha Bhanu, G. Swapna, and Prof.B. Kesava Rao,*

Turmeric,curcumin lona L of Zingiberaceae family is a widely cultivated spice in

India and other Asian countries.Turmeric is rich in Curcuminoids, they are recognized for
their broad spectrum of Biological activities. Curcuminoids vary in their chemical structure,
physiochemical characteristics aswell as the functional properties. Turmeric comes from the
root of curcuma longa plant and has tough brown skin and orange flesh. Turmeric has long
(RACPABS - 2020)
been used as powerful anti inflammatory in both Indian and Chineese medicine.Turmeric is
also known as “Indian saffron” because of its deep yellow orange color. In this paper we are
discussing about the preliminary chemical investigation of Curcuma longa L.
Keywords: Curcumin, Anti inflammatory Activity, Zingiberaceae.
PP-64: ISOLATION OF LYCOPENE FROM SOLANUM

LYCOPERSICUM
M. Supriya, P Asha Bhanu, G Swapna and Prof.B. Kesava Rao,*

Lycopene is a carotenoid pigment and Phytochemical found in tomatoes. Tomato is

the edible, often red, berry of the plant solanum Lycopersicum belongs to the family of
Solanaceae. The species originated in western South America and Central America. The
antioxidant properties of Lycopene have attracted several researchers. Extraction and
isolation can be done with Methanol-cc14 method and Nacl method. Lycopene is one of 600
carotenoids found in nature and easily identified in tomatoes which help in reduced risk of
cancer, coronary and cardiovascular diseases.
PP-65: SIMULTANEOUS UV AND FIRST ORDER DERIVATIVE

SPECTROPHOTOMETRIC ESTIMATION OF IMIPENEM AND
CILASTATIN IN PHARMACEUTICAL DOSAGE FORMS
Masimukku Siva Kishorea,B* And Dittakavi Ramachandrana

a
Department of Chemistry, Acharya Nagarjuna University, Guntur, AP, India.
b
Department of Chemistry, K.B.N. College, Vijayawada, AP, India.
Mobile No: +91 98661 43345, Email: siva.masimukku@gmail.com
To develop simple, rapid, accurate, precise and economical UV spectrophotometric

methods for the simultaneous determination of imipenem and cilastatin in combined tablet
dosage form using simultaneous equation method and first order derivative method. The
methods are based on the simultaneous equations for analysis of both the drugs using
Methanol: Acetate buffer pH 5.2 in the ratio of 8:2 (v/v) as solvent. Imipenem has
absorbance maxima at 226 nm whereas cilastatin at 217 nm. The linearity was obtained in
(RACPABS - 2020)
the concentration range of 3-18 μg/mL for both imipenem and cilastatin. The concentrations
of the drugs were determined by using the simultaneous equation method and regression
equation in simultaneous equation method and first order derivative method respectively. The
developed methods were found to be simple, accurate and precise and were applicable for the
simultaneous determination of imipenem and cilastatin in the pharmaceutical tablet dosage
form. The results of analysis have been validated statistically and by recovery studies.
PP-66: SYNTHESIS OF SOME NEW 2-BENZYLOXY-5-ALKYNE

SUBSTITUTED PYRIMIDINES THROUGH SONOGASHIRA
COUPLING
O. Surendranatha Reddy, M. Baby Ramana, T. Vijaya Durga, Bollikolla Hari Babu*

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, AP,
India-522510, ph: 0863-2346575. E-mail address: dr.b.haribabu@gmail.com
Sonogashira coupling between diversely substituted 2-benzyloxy-5-bromo

pyrimidines and various aryl and aliphatic alkynes in the presence of catalytic amount of
PdCl2(PPh3)2 with diethylamine and CuI as a co catalyst in THF at 60 oC affords 2-
benzyloxy-5-alkyne substituted pyrimidines in good yields (85-95%). The cytotoxic activity
of resultant compounds were evaluated against A549and HCT116 human cancer cell lines.
Some of the 2-benzyloxy-5-alkyne pyrimidines have shown moderate in vitro cytotoxic
activity against A549 (Human lung adenocarcinoma) cell line.
PP-67: SYNTHESIS, STRUCTURAL CHARACTERIZATION AND

ANTIMICROBIAL ACTIVITY ZINC OXIDE NANOPARTICLES
1
*T.Aparna, 2M.Krishna Murthy,2 K Surendra babu
1
Department of chemistry, RGUKT (APIIIT), Nuzivid-521202,India
2
Department of chemistry, S.V.R.M.College, Nagaram-52226, India
*Corresponding Author:aparnatirumalasetti@gmail.com
Zinc oxide (ZnO) has very particular physical and chemical properties, such as high
chemical stability, high electrochemical coupling coefficient, wide radiation absorption
range, high photo stability and biodegradability; properties that make it a multifunctional
materiali. Due to the aforementioned properties, ZnO is widely used in many areas ranging
from the pharmaceutical industry, to chemistry, tire manufacturing, additives in ceramics and
paints, as well as in agriculture where it is incorporated into fertilizers by its antibacterial
(RACPABS - 2020)
activityii.The objective of this work was to develop a methodology of synthesis and

characterization ofZnO nanoparticle on laboratory scale in a simple and scalable way. In
addition, the antimicrobial activity of ZnOnanoparticles was determined according to their
minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).
ZnO nanoparticles were synthesized by using Zn(CH3CO2)2·2H2O as a precursor, citric acid
(C6H8O7) as chelating agent, which gave way to the formation of the metal chelate; and
ethylene glycol (CH2OH)2 as a solvent, in a metal:citric acid :ethylene glycol molar ratio
equal to 1:4:16. To adjust the pH, ammonium hydroxide (NH4OH) was usediii. The variables
of the process were the times and temperatures of the calcination process of the product
obtained from the synthesis. ZnO particle characterised by X-ray diffraction tests (XRD),
scanning electron microscopy (SEM), Dynamic light scattering (DLS), Transmission electron
microscopy (TEM) and FTIR spectroscopy analysis. The nanoparticles synthesized at the
different calcination conditions analyzed through XRD and SEM confirmed the presence of
100% ZnO in the form of zincite. Through the DLS analysis, a minimum effective diameter
of approximately 23 nm was obtained for the calcination conditions of 700°C and 3 hours.
This represents the best synthesis condition. The TEM analyses allowed corroborating the
particle size obtained by DLS, as well as determining that the synthesized nanoparticles have
a spherical shape and are dispersed, although they tend to agglomerate in certain areas. The
FTIR analysis allowed to verify the formation of ZnO by means of the comparison of the
spectra of the samples of ZnO synthesized with respect to a commercial sample, the values of
correlation coefficients were greater than 0.98.The tests carried out demonstrate a strong
antimicrobial activity of ZnO NP against both the Gram negative bacteria E. coli and for the
Gram positive S. aureus being that the MIC and MBC values determined in both models are
considerably lower than those reported in the literatureiv for zinc oxide nanoparticles under
experimental conditions similar to those developed in this work.
PP-68: INTERNATIONAL CONFERENCE ON RECENT ADVANCE IN

CHEMICAL, PHARMACEUTICAL &BIOLOGICAL SCIENCES SUB THEME:
GREEN CHEMISTRY
1. B.Sujatha, M.Sc 2.P.Neeradha, M.Sc 3.Dr B.Sridevi M.Sc ,Phd

Lecturer in Chemistry. Lecturer in Chemistry. Lecturer in Chemistry
S.K.R Gov Degree college VRS&YRN College Y.A .Gov Degree college
Gudur Chirala Chirala
Green chemistry, also called sustainable chemistry, is a philosophy of chemical

research and engineering that encourages the design of products and processes that minimize
the use and generation of hazardous substances. seeks to reduce the negative impact of
(RACPABS - 2020)
chemistry on the environment by preventing pollution Whereas environmental chemistry is

the chemistry of the natural environment, and of pollutant chemicals in nature, green
chemistry at its source and using fewer natural resources. As a chemical philosophy, green
chemistry applies to organic chemistry, inorganic chemistry, biochemistry, analytical
chemistry, physical chemistry and even chemical engineering. While green chemistry seems
to focus on industrial applications, it does apply to any chemistry choice. Click chemistry is
often cited as a style of chemical synthesis that is consistent with the goals of green
chemistry. The focus is on minimizing the hazard and maximizing the efficiency of any
chemical choice. Green chemistry, also called sustainable chemistry, is a philosophy of
chemical research and engineering that encourages the design of products and processes that
minimize the use and generation of hazardous substances. Whereas environmental chemistry
is the chemistry of the natural environment, and of pollutant chemicals in nature, green
chemistry seeks to reduce the negative impact of chemistry on the environment by preventing
pollution at its source and using fewer natural resource. One of the most important new
aspects of chemistry is the development of Green Chemistry. Supported by the American
Chemical Society, the USEPA and other Federal agencies, Green Chemistry has emerged as
an important aspect of all chemistry Green Chemistry is the design of chemical products and
processes that reduce or eliminate the use and generation of hazardous substances.
Green Chemistry is based on Twelve Principles. 12 Principles of Green Chemistry
 Prevent Waste
 Atom economy
 Less Hazardous Synthesis
 Design Benign Chemicals
 Benign Solvents & Auxiliaries
 Design for Energy Efficiency
 Use of Renewable Feedstocks
 Reduce Derivatives
 Catalysis(vs.Stoichiometric)
 Design for Degradation
 Real-Time Analysis for Pollution Prevention
 Inherent Benign Chemistry for Accident Prevention
Science and business move forward through discovery and innovation. The design
principles of green chemistry and engineering provide researchers with new tools to create
processes and products that cost less, and are healthier and more sustainable.
(RACPABS - 2020)
PP-69: GREEN SYNTHESIS OF GRAPHENE-HYDROXYAPATITE

NANOCOMPOSITES WITH IMPROVED MECHANICAL
PROPERTIES FOR BONE IMPLANT MATERIALS
Syed Akhil,1 and Basavaiah Chandu1*

Presenting Author: e-mail: chandupcu@gmail.com
1 Dept. of Nanotechnology, ANU College of Sciences, ANU, Guntur-522510
An in situ synthesis of reduced graphene oxide-hydroxyapatite (RGO-HA)

nanocomposite via a green reduction protocol is presented here. Three different samples with
0.5, 1.0 and 1.5 % of graphene in HA are synthesized by reducing the precursor solution with
custard apple leaf extract. The XRD analysis showed the formation of standard HA matching
with the JCPDS card No.09-0432. Raman and UV-Vis investigations confirmed the
formation of graphene in this green chemistry method. The 10x5 mm sized discs of the RGO-
HA nanocomposite are prepared and investigated for their mechanical properties. Nano-
indentation analysis showed improved mechanical properties with Young’s modulus of 30.2
GPa and hardness of 1.106 GPa for 1.5 % RGO-HA nanocomposite. The nanocomposite also
showed superior wear volume (42.06 x 10-21 m3) and coefficient of friction (0.609) compared
to pure HA. Therefore, the prepared 1.5 % RGO-HA nanocomposite may find applications in
bio-implants such as bones and hard tissues.
(RACPABS - 2020)
PP-70: DESIGN, SYNTHESIS AND BIOLOGICAL EVALUATION OF

SULPHONAMIDE DERIVATIVES OF BENZOFURAN-
IMIDAZOPYRIDINES AS ANTICANCER AGENTS
I. Sreenivasa Murthya , R. Sreenivasulub , and R. Ramesh Rajua *

a
Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, 522510,
b
Nehru Technological University, Kakinada, 533003, Andhra Pradesh, India.
*Corresponding author Email: rrraju1@gmail.com
We have designed and synthesized a novel library of sulphonamide derivatives of

benzofuran-imidazopyridines (9a-j) and their structures were characterized by 1HNMR,
13
CNMR and mass spectral analysis. All these compounds were screened for their anticancer
activity against four human cancer cell lines such as breast cancer (MCF-7), lung cancer
(A549), colon cancer (Col-205) and ovarian cancer (A2780) by employing MTT assay. The
results are expressed with IC50 µM, which indicated that all of the compounds showed good
to moderate activity on tested cell lines. Among all synthesized compounds, compounds 9a,
9c, 9h, 9i and 9j displayed more significant anticancer activity than etoposide.
O OMe
H HO OH
Br B
MeO H2N Br
IBD, dioxane N
rt, 10 min
O H2N N N N
3 4 O MeO OMe
5 H 6
OMe
Pd(PPh3)4, dioxane
Cs2CO3, reflux, 7h
OMe OMe OMe OMe

OMe O Cl OMe
S
MeCN, Cs2CO3 O
N N OMe
OMe rt, 12h
O Ar O
N N N
N H 7
O 8a-j
O S
Ar 8a; R = phenyl 8a; R = phenyl

8b; R = 3,5-dichlorophenyl 8b; R = 3,5-dichlorophenyl
9a-j 8c; R = 4-methoxyphenyl 8c; R = 4-methoxyphenyl
8d; R = 4-chlorophenyl 8d; R = 4-chlorophenyl
8e; R = 4-bromophenyl 8e; R = 4-bromophenyl
8f; R = 4-nitrophenyl 8f; R = 4-nitrophenyl
8g; R = 4-cyanophenyl 8g; R = 4-cyanophenyl
8h; R = 4-methyl 8h; R = 4-methyl
8i; R = 2,6-dimethylpyridine-4-yl 8i; R = 2,6-dimethylpyridine-4-yl
8j; R = 4,5-dimethylthiophene-2-yl 8j; R = 4,5-dimethylthiophene-2-yl
(RACPABS - 2020)
PP-71: DESIGN, SYNTHESIS AND ANTICANCER EVALUATION OF

1,2,4-THIADIAZOLE LINKED PYRIDONE DERIVATIVES
Neerada Perupogua,b, Syed Khasim Sharifa, and Dittakavi Ramachandrana*

a
Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar,
Andhra Pradesh 522510, India.
b
Department of Chemistry, VRS & YRN College, Chirala, Andhra Pradesh, 523115, India.
*Corresponding author Email: dittakavirc@gmail.com
Abstract: We have synthesized a new series of 1,2,4-thiadiazole linked pyridone (9a-j)

derivatives and their structures were determined by 1HNMR, 13CNMR and mass spectral
data. Further, all compounds were investigated for their anticancer activity against a panel of
human cancer cell lines including MCF-7 (breast), A549 (lung), Colo-205 (colon) and A2780
(ovarian) by using MTT assay. All these tested compounds exhibited outstandingly
anticancer activities against all cell lines, and these results were comparing with clinical agent
etoposide (positive control). Among all synthesized compounds, six compounds9b, 9c, 9d,
9e, 9i and 9j were showed more potent anticancer activity than positive control. In which
compound 9b was a displayed remarkable anticancer activity.
CHO O
O
Piperidine, EtOH MeO OMe
MeO OMe MeO OMe reflux, 6h 5
OMe
3 OMe
OMe OMe
4
O
t-BuOK, DMSO,
NH2
50 oC, 3h N
H
6 O
ClH.HN NH2 O
O 8a-j NH
MeO R MeO OMe
NH
N
OMe K3PO4, S, DMSO 7 OMe
R N S 130 oC, 12h
MeO OMe OMe
9a-j
OMe
MeO
9a; R = H 8a; R = H
9b; R = 3,4,5-trimethoxy 8b; R = 3,4,5-trimethoxy
9c; R = 4-methoxy 8c; R = 4-methoxy
9d; R = 4-chloro 8d; R = 4-chloro
9e; R = 4-bromo 8e; R = 4-bromo
9f; R = 4-nitro 8f; R = 4-nitro
9g; R = 4-methyl 8g; R = 4-methyl
9h; R = 4-cyano 8h; R = 4-cyano
9i; R = 3,5-dimethyl 8i; R = 3,5-dimethyl
9j; R = 4-(dimethylamino) 8j; R = 4-(dimethylamino)
(RACPABS - 2020)
PP-72: PURIFICATION FREE AND CHEMOSELECTIVE ACYLATION

OF NON-NUCLEOPHILIC N-HETEROCYCLES USING OXYMA AND
BENZOTRIAZOLE ACTIVATIONS
T. Bhagya Kumar1 & M. Manoranjani2

1 Department of Chemistry, KBN College, Vijayawada-1
2 Department of chemsitryP.B. Siddhartha College of Arts and Science, Vijayawada
e-Mail: tbhagyakumar@gmail.com
A method for acylating the N heterocyclic compounds with particular reference

to indoles has been developed under milder conditions without the column
chromatography and work up. The reagents and the solvents involved are milder and
the conditions are very much green. Such a scalable process is also economic and can
serve the need of the industry in clean isolation of the products. The yields for
complicated and much less nucleophillic indoles is encouraging. The substrate scope is
wider and tolerant for many functional groups. In none of the cases, di and tri acylated
products are seen.
(RACPABS - 2020)
PP-73: DESIGN, SYNTHESIS AND BIOLOGICAL EVALUATION OF

CHALCONE LINKED THIAZOLE-IMIDAZOPYRIDINE
DERIVATIVES AS ANTICANCER AGENTS
Vellanki Ragha
Suma, Reddymasu Sreenivasulu, Madala Subramanyam,4Kuppili Ram Mohan Rao1*
1,2 3
1
Department of Chemistry, GITAM Institute of ScienceGandhi Institute of Technology and
Management (Deemed to be University), Visakhapatnam – 530045, Andhra Pradesh, India
2
Department of Chemistry, Government Degree and PG College, Bhadrachalam – 507111,
Telangana, India
3
Nehru Technological University, Kakinada – 533003, Andhra Pradesh, India
4
Department of Chemistry, SR and BGNR Govt. Arts and Science College (Autonomous),
Khammam - 507002, Telangana, India
*Corresponding author Email:rammohanrao.k@gmail.com
A new novel library ofchalcone linked thiazole-imidazopyridine (12a-j) derivatives

were designed, synthesized and their structures characterized by 1HNMR, 13CNMR and mass
spectral data. Further, all compounds were tested for their anticancer effects on four human
cancer cell lines including MCF-7 (breast carcinoma), A549 (lung carcinoma), DU-145
(prostate carcinoma) and MDA MB-231 (breast carcinoma) by employing MTT method, and
etoposide used as positive control. Among them, compound12b displayed more potent
anticancer activitieagainst four cancer cell lines than positive control.
o
CHO CuBr, EtOH, 60 C, 8h N
N NH2 N
3 4 CHO 5
Br NH2OH.HCl, DMSO
O 90 oC, 2h
N NaSH, MgCl2.6H2O N
N DMF, 40 o
C, 90 min N
MeO OMe 7
OMe S 6 CN
H2N
8 CHO
Ethanol, rt, 1h
reflux, 20 min
MeO
S OMe
MeO N EtOH, SeO2, reflux
N N N
N
24h N OMe
MeO 10
S
OMe
9
O
11a-j EtOH, aq KOH

11a; R = H R
11b; R = 3,4,5-trimethoxy rt, 12h
11c; R = 3,5-dimethoxy
11d; R = 4-methoxy
11e; R = 4-chloro N
N R
11f; R = 4-bromo
11g; R = 4-nitro
11h; R = 3,5-dinitro
11i; R = 4-cyano N
S
11j; R = 4-methyl O
MeO 12a-j
MeO
OMe
12a; R = H
12b; R = 3,4,5-trimethoxy
12c; R = 3,5-dimethoxy
12d; R = 4-methoxy
12e; R = 4-chloro
12f; R = 4-bromo
12g; R = 4-nitro
12h; R = 3,5-dinitro
12i; R = 4-cyano
12j; R = 4-methyl
(RACPABS - 2020)
PP-74: DESIGN, SYNTHESIS AND ANTICANCER ACTIVITIES OF

1,2,4-THIADIAZOLE LINKED 1,3,4-THIADIAZOLE-CARBAZOLES
ANALOGUES
EeduriRamya Devi1,2, Reddymasu Sreenivasulu2, KoyaPrabhakara Rao1, Ratnakaram

Venkata Nadh3, MalladiSireesha*1
1
Division of Chemistry, Department of Science & Humanities, Vignan’s Foundation for
Science, Technology and Research (Deemed to be University), Vadlamudi - 522 213,
Guntur, Andhra Pradesh, India.
2
Nehru Technological University, Kakinada – 533 003, Andhra Pradesh, India.
3
Department of Chemistry, Gandhi Institute of Technology and Management (Deemed to be
University), Bangalore campus – 561 203, Karnataka, India.
*Corresponding author Email: sireesha_malladi@yahoo.co.in
We have synthesized a new library of 1,2,4-thiadiazole linked 1,3,4-

thiadiazolecarbazole(12a-j) and were evaluated for their anticancer activities against four
human cancer cell lines such as MCF-7 (breast cancer), A549 (lung cancer), Colo-205 (colon
carcinoma)and A2780 (ovarian carcinoma) by employing MTT assay with etoposide as a
referencestandard. It was noticeable from the preliminary screening that most of the tested
compounds exhibited significantly anticancer activity on tested cell lines. Among them, the
compound12b showed potent anticancer activities against MCF-7, A549, Colo-205 and
A2780 cell lines with IC50 values of 0.035±0.0051 μM, 0.012±0.0036 μM, 0.014±0.0011
μM and0.017±0.0029 μM respectively.
(RACPABS - 2020)
PP-75: DETERMINATION OF 3-CHLORO-1,2-PROPANEDIOL IN

IOHEXOL DRUG SUBSTANCE USING HIGH PERFORMANCE
LIQUID CHROMATOGRAPHY WITH REFRACTIVE INDEX
DETECTOR
N. Sanni Babu1, G. Sailaja2, M. Santha Kumari3, B. Hari Babu1

1
Department of Chemistry, Acharya Nagarjuna University, NNagar, Guntur-522510, AP-India
2
Department of Chemistry, Singareni Collieries Women’s Degree College, Kothagudem, Telangana,
India
3
Department of Chemistry, Govt. College for Women, Srikakulam, AP- India
Iohexol is used in intrathecal administration in adults during myelography and in

enhancement of contrast during computerized tomography. 3-Chloro-1,2-propanediol is used
during the chemical synthesis of Iohexol. The quantification of residual 3-chloro-1,2-
propanediol in Iohexol drug substances is a significant analytical control because of necessity
to determine Iohexol purity and the toxicity of 3-chloro-1,2-propanediol. A reversed phase
high performance liquid chromatography method is developed wherein refractive index
detector is used to quantify trace levels of residual 3-chloro-1,2-propanediol in Iohexol drug
substance. The separation, detection and quantification were achieved using a Symmetry
shield reverse phase C18 analytical column (250 mm × 4.6 mm, 5 µm particle size). Linearity
was seen in the concentration range of 1.29 to 38.77 µg ml-1 for 3-chloro-1,2-propanediol.
The correlation coefficient for 3-chloro-1,2-propanediol was 0.9998. Following international
conference on harmonization guidelines, sensitivity (LOD - 0.43 µg ml-1; LOQ - 1.29 µg/ml),
percent recoveries (99.30% - 100.89%), relative standard deviation (1.07% - 1.42%),
robustness (˂2% RSD) for the method were determined.
PP-76: A NEW STEROID ISOLATED FROM LEUCAS CEPHALOTES
K.Kavitha Priya*1; V.Anuradha1,2 ; G.Jayalakshmi1& S.Ratnakumari2

*,1
Department of Chemistry, Vignan Degree College, Palakaluru,Guntur, Andhra
Pradesh,Pin-522005,India.
2
Vignans Nirula Institute of Technology & Science for women, Palakaluru,Guntur,
AndhraPradesh,Pin-522005, India.
From the flowers of Leucas cephalotes, a new steroidal triterpenoid was isolated. Its
structure was elucidated as (Z)-17-(4,4-dimethylhept-2en-2-yl)-8,10,14-trimethy-2,3,4,5,6,
7,8,10,12,13,14,15,16,17-tetradecahydro-1H-cyclopent [a] phenanthren-3-ol, basing on
spectral data. This is the first report from Leucas cephalotes in particular, from Lamiaceae
(RACPABS - 2020)
family and in nature in general. Earlier reports reveal isolation and characterization of
essential oils [1]. -sitosterols and stigmasterols were reported in major percentages [2] in
Leucas cephalotes. The research was carried out in research lab of Vignan degree
college,Pedapalakalur,Guntur. The Process how compound was obtained is by extraction, gel
washing of extract and column chromatography and recrystallisation. The extract was
prepared from the air dried flowers of Leucas cephalotes using soxhlet apparatus and ethanol.
The extract was then washed using Solvents hexane,benzene,ethyl acetate,chloroform and
methanol respectively. The hexane wash is taken and concentrated and subjected to fractional
crystallization using methanol. A solid was separated and filtered. Filtrate is taken and
subjected to column chromatography after observing spots with different Rf values.
Compound was obtained from Hexane:Ethyl acetate (9:1) fraction after recrystallisation. The
compound was obtained as a white colour crystalline needles and showed single spot when
observed in TLC. The compound gave positive result for Solkowski reaction [3] and
Libermann-Burchard reaction [4] confirming it is a steroid. The spectral data was obtained
from IICT,Hyderabad. It is named as Leuasterenol and the structure was established using
Uv, IR, Proton NMR, 13CNMR and EI mass fragmentation as (Z)-17-(4,4-dimethylhept-2en-
2-yl)-8,10,14-trimethy-2,3,4,5,6,7,8,10,12,13,14,15,16,17-tetradecahydro-1H-cyclopent[a]
phenanthren-3-ol. This is the first report from Leucas cephalotes and from family Lamiaceae
and nature.
PP-77: A NOVEL PYRIDYL ESTER ISOLATED FROM LEUCAS

CEPHALOTES
Kavitha Priya Kummaraa, Anuradha Vejendlaa*

a
Department of Chemistry, Vignan Degree College, Guntur,Andhra Pradesh,
pin-522004,India.
a*
Department of BS&H, VNITSW, Guntur,Andhra Pradesh,pin-522004,India.
From the flowers of Leucas cephalotes pyridine-3-carboxylic acid ester was isolated.
Its structure was established as 2’,5’- dihydroxy-3’-pyridyl-(2,5, dihydroxy)-pyridinyl 3-
carboxylate on the basis of spectroscopic data. This is the first report from Leucas cephalotes
in particular and Labiatae family in general. The compound was named as above basing on
the spectral data obtained from IICT,Hyderabad. Uv,IR,Proton NMR ,13CNMR and EI mass
fragmentation spectras helped us in elucidating the structure of the compound. The whole
work was carried out in chemistry research lab of Vignan degree college,
Pedapalakalur,Guntur. Leucas cephalotes is the plant from which compound was isolated.
The plant belongs to family Lamiaceae and the plant was chosen by us as it has many
medicinal values and particularly the flowers were chosen by us. Antifilarial, antibacterial,
(RACPABS - 2020)
antidiabetic and hepato-protective activities were reported from the whole plant of L.
cephalotes [1]. The syrup of flowers mixed with honey are useful in diagnostic remedies of
cough and colds [2,3]. The plant has been reported to exert multiple biological effects
pharmacologically like antioxidant [4], analgesic,anthelmintic and antimicrobial activities.
The method how we isolated the Compound is by extraction, gel wash, PTLC and
recrystallisation. The flowers were separated from the plant and air-dried. The air –dried
material is repeatedly extracted in a soxhlet apparatus using ethanol for many days. The
ethanol extract was then washed, which we refer to as gel filtration using solvents
hexane,benzene, ethyl acetate,chloroform and methanol respectively. The hexane wash is
taken and it is subjected to PTLC using plates of 19x16 cms size and developing solvent was
Benzene:Ethyl acetate (8:2). Four bands were observed in Uv chamber. The four layers were
separated and the Compound was obtained from the second layer after recrystalisation and it
is a light blue coloured. It is named as 2’,5’-dihydroxy-3’-pyridyl,(2,5-dihydroxy)-pyridinyl-
3-carboxylate. This is the first report of this Compound from the plant Leucas cephalotes ,
from the family Lamiaceae (Labiatae) and from nature.
PP-78: COLORIMETRIC DETERMINATION OF BENDAMUSTINE
K. Kiran Kumar1and R.VenkataNadh2*

1
Department of Chemistry, KBN College, Vijayawada-520001. India.
2
A simple and sensitive visible spectrophotometric methodshas been developed for the
determination of Bendamustine in bulk drug and pharmaceutical formulations. The developed
methods involve the formation of colored complex of Bendamustine with PCA in acid
medium, exhibiting absorption maxima at 520and and obeying Beer’s law in the
concentration range of 2.0–12.5µg/ml with good correlation. Statistical analysis of the results
of the proposed methods reveals high accuracy and good precision. The proposed method
could be successfully extended to the commercial pharmaceutical formulations containing
Bendamustine.
(RACPABS - 2020)
PP-79: BIOANALYTICAL LC-MS/MS METHOD DEVELOPMENT

AND VALIDATION OF NOVEL ANTIDIABETIC DRUGS IN RAT
PLASMA AND ITS APPLICATION TO PHARMACOKINETIC AND
ORAL BIOAVAILABILITY STUDIES
T.Subrahmanyam1, V.Anuradha*2, BSN.Murthy3, G.R.Satyanarayana4

Department of Chemistry, Sir.C.R.Reddy College, P.G. Courses, ELURU, Andhra Pradesh,
INDIA-534007
*Department of Chemistry, Vignan Degree College, GUNTUR, Andhra Pradesh, INDIA-534007
Email id : subbutlr@gmail.com
A sensitive and selective LC-MS/MS method has been developed and validated for
antidiabetic drugs in rat plasma using an internal standard. The API 4000 triple quadrupole
LC-MS/MS system was operated under multiple reactions monitoring mode using
electrospray ionization technique in positive mode. The sample processing method involves
2-step liquid-liquid extraction using n-hexane as an extracting solvent. The analyte was
chromatographed on C18 18, waters column (3.5 µm, 4.6 mm i.d.×15 mm) with guard using
acetonitrile and ammonium acetate buffer (pH 2.5 with OPA) in 50:50 (v/v) composition at a
flow rate of 1.00 mL min-1. The chromatographic run time was 10 min. Calibration curve
shows linearity over concentration range 1.56-200 ng mL-1. The lower limit of detection was
0.39 ng mL-1 and lower limit of quantitation was 1.56 ng mL-1. The inter- and intra-day
accuracy and precision were found to be within the assay variability limits as per US FDA
guidelines. The absolute recovery was found to be >90%. Antidiabetic drugs does not show
any stability problems as it was stable at room temperature for 12 h. Antidiabetic drugs was
also stable up to 3 freeze-thaw cycles and can be stored up to 30 days at -60°C. The assay
was successfully applied to both oral (40 mg kg-1) and intravenous (10 mg kg-1)
pharmacokinetic studies in male Sprague-Dawley rats.
Reference
1. Gelboin, Harry V, Kristopher W, Gonzalez, Frank J, Yang, Tian J (1999). Inhibitory
monoclonal antibodies to human cytochrome P450 enzymes. A new avenue for drug
discovery. Trends in Pharmacological Sciences. 20 (11): 432-8.
2. Tansey EM, Catterall PP (1994). Monoclonal antibodies: a witness seminar in
contemporary medical history. Med. Hist. 38 (3): 322-7.
3. Hayes, James; Richardson, Ann; Frampton, Chris (2013). Population attributable risks
for modifiable lifestyle factors and breast cancer in New Zealand women. IMJ. 43 (11):
1198-1204.
4. Sengupta, Nandini; Sahidullah, Md; Saha, Goutam (2016). Lung sound classification
using cepstral-based statistical features. Computers in Biology and Medicine. 75 (1):
118-129.
(RACPABS - 2020)
PP-80: Development and Validation of Stability Indicating RP-HPLC

Method for Simultaneous Estimation of Epalrestat and Pregabalin in Bulk
and Tablet Dosage Form
Md.Shabana Sulthana1
Research Scholar, Dept. of Chemistry, Acharya Nagarjuna University,
Nagarjuna Nagar – 522510, AP, India.
G.Ratna Babu
Research Scholar, Dept. of Chemistry, Acharya Nagarjuna University,
Nagarjuna Nagar – 522510, AP, India.
The purpose of the investigation was to develop a new RP-HPLC method for
simultaneous estimation of Epalrestat at and Pregabalin in pharmaceutical dosage forms.
Chromatography was carried out on Kromasil 250 C-18 column (4.6 x 250mm, 5μ particle
size) with a isocratic mobile phase composed of ortho phosphoric acid buffer, Acetonitrile,
47:53v v-1) at a flow rate of 1 mL min-1. The column temperature was maintained at 30°C
and the detection was carried out using a PDA detector at 210 nm. Validation parameters
such as system suitability, linearity, precision, accuracy, specificity, limit of detection (LOD),
limit of quantification (LOQ), Stability of sample and standard stock solutions and robustness
were studied as reported in the International Conference on Harmonization guidelines. The
retention times for Epalrestat and Pregabalin and were 2.575 min and 3.406 min respectively.
The percentage recoveries of Epalrestat and Pregabalin were 99.29 % and 100.34 %
respectively. The relative standard deviation for assay of tablets was found to be less than 2
%. The method was fast, accurate, precise and sensitive hence it can be employed for routine
quality control of tablets containing both drugs in quality control laboratories and
pharmaceutical industries.
Keywords: Epalrestat, Pregabalin, High performance liquid chromatography, Acetonitrile,
Orthophosphoric acid, Terbutaline
(RACPABS - 2020)
PP-81:SYNTHESIS
81:SYNTHESIS AND CHARACTERIZATION OF
PHENANTHROIMIDAZOLES
Thota Ambika,, Final year M.Sc Inorganic chemistry, Acharya Nagarjuna University,
Guntur.
Dr.V.Krishnan,, Associate professor, National Institute of Science Education and Research,
Bhubaneswar.
Imidazoles are nitrogen containing heterocyclic compounds found applications in

diverse fields. In recent years, considerable efforts have been paid to the development of
imidazole based luminescent materials due to their ease of synthesis and their potential
application in the field of Organic Light Emitting Diodes (OLEDs). For instance, 9,10 9,10-
phenanthroo based imidazoles have attracted tremendous interest to produce efficient deepdeep-
blue OLEDs. This development of phenanthroimidazole for blue OLEDs is studied due to its
electroluminescent properties. These efficient blue-emitting
emitting materials are extremely eessential
for commercialization of white OLEDs. However, despite the many advantages of displays
built with OLED, there is still a need to improve their efficiency. Therefore, the current
research directions are primarily focused on improving the luminous eefficiency. The
compounds which enjoy growing interest for use as blue light emitters are
phenanthroimidazoles. These are synthesized based on Debus-Radziszewski
Debus Radziszewski reaction of
aldehyde, amine, diketone. Starting materials such as amine and diketone are commer
commercially
available and some aldehydes are prepared according to literature. All these reactions are
carried out under nitrogen atmosphere. The structures of aldehyde and phenanthroimidazoles
are confirmed by 1H-NMR
NMR and 13C-NMR analysis.
Keywords: OLEDs, Imidazoles,
azoles, Phenanthroimidazoles, Aldehydes, Amines, Diketones.

522510, Guntur
Guntur, A.P. 221
(RACPABS - 2020)
PP-82: Isolation and Biological activity of Nybomycin
B. Meenakshi, A. Mallikarjuna rao, Dr. D. Ramachandran*.

Dept of Chemistry, Acharya Nagarguna University, Nagarjuna Nagar-522510, A.P., India.
Nybomycin is a broad antiphage spectrum, it was isolated from different environment

such as Missouri soil, terrestrial soil, surface of carpenter ants and marine sediments. The
concentrate was prepared form culture liquid were found to have no effect on PR8 influenza
or on SK polio virus. In further studies a colorless crystalline compound was isolated from
the culture liquid and mycelium of A 717 having antiphage and antibacterial activity.
Nybomycins are pyridoquinolinedione-based metabolites of Streptomyces. These secondary
metabolites are selective inhibitors of a quinoline-resistant Ser84Leu mutantDNA gyrase
(GyrA). Nybomycins are also active against both proliferating and dormant
Mycobacteriumtuberculosis,some nybomycins are shows potentcancer cell line cytotoxicity
and also inhibit cell division cycle 25 (CDC25) phosphatases which is a key regulators of the
eukaryotic cell cycle and are highly overexpressed in many cancers. the isolation and
structure elucidation of three new nybomycins (nybomycins 1−3) and six previously reported
metabolites (nybomycin, 4; deoxynyboquinone, Bioactivity studies revealed compounds 3
and 5 to afford moderate (3) to potent (5) cancer cell line cytotoxicity and weak to moderate
anti-Gram-(+) bacterial activity.
Key words: Nybomycins , cytotoxicity, Streptomyces.
(Ref: J. Nat. Prod. 2019, 82, 12, 3469-3476)
Isolated Nybomycin and its derivatives from streptomycessp. AD-3-6
(RACPABS - 2020)
PP-83: Synthesis and Anticancer Evaluation of 1, 2, 4-Thiadiazole bearing

combretastatin-A4 Derivatives as Anticancer Agents
S. Premalatha, G. and D. Ramachandran*

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Andhra
Pradesh, 522510, India.
*Corresponding author Email:dittakavirc@gmail.com
A new series of 1,2,4-thiadiazole bearing combretastatin-A4 (8a-j) derivatives were

designed and synthesized. All these compounds tested for their anticancer activities against a
panel of human cancer cell lines such as breast cancer (MCF-7), lung cancer (A549), cervical
cancer (Hela) and breast cancer (MDA MB-231). The clinical drug like combretasttin-A4
used as standard reference. Among all compounds, six compounds 8b, 8c, 8d, 8e, 8i and 8j
were displayed superior anticancer activities to compare with standard reference. Especially,
compound 8b was showed strongest anticancer activity.
Keywords: Combretastatin-A4, combretastatin A-4 phosphate (CA-4P), cefozopram and
anticancer activity.
PP-84: Evaluation of flavone-8-acrylamide derivatives as Multifunctional

anti-Alzheimer agents and their Mechanism of Action with
Acetylcholinesterase
K. Yelamanda Rao1, S. Jeelan Basha1, P. Mohan, Z. Raveendra Babu1, K. Mounika2,

S. Rajagopal2, A. G. Damu1*
1
Department of Chemistry, School of Physical Sciences, Yogi Vemana University, Kadapa.
2
Department of Plant Sciences, School of life Sciences, University of Hyderabad, Hyderabad.
yelamanda10@gmail.com
Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized

by cognitive impairment, loss of memory and language deterioration and become one of the
biggest global public health issues around the world [1]. In a search for novel multifunctional
anti-Alzheimer agents, a congeneric set of seventeen flavone-8-acrylamide derivatives were
synthesized and evaluated for their cholinesterase inhibitory, antioxidant, neuroprotective and
modulation of Aβ aggregation activities. [2]. The target compounds showed effective and
selective inhibitory activity against the AChE over BuChE. In addition, the target compounds
also showed moderate antioxidant activity and strong neuroprotective capacities, and
accelerated dosage-dependently the Aβ aggregation. The binding constant and free energy
(RACPABS - 2020)
values coincide for both biophysical and computational analysis clearly suggesting that
AChE-flavone complex is more stable. Altogether, these results suggest that flavone and
cyanoacetamide hybrid compounds are an encouraging starting point for new ways in the
development of multifunctional compounds for the treatment of AD.
HO OH CHO
HO O H3CO O
1
iv) AcOH, HMTA, MW, 300W, 3
i) ZnCl2, AcOH, 145-1500C; 2
O 8 min, 10% HCl, 5min O
ii) K2CO3, acetone, PhCOCl, 20h, reflux v) (CH3)2SO4, dry acetone, R1
0
iii) H2SO4, 4h, 5 C; 5% K2CO3, conc HCl K2CO3, 4h, reflux O N
R2
NC
OEt R R NH, EtOH R1 3, Et3N, EtOH
NC 1 2 H3CO O
N
O NC R2
4 O 6
5
O
References
1. W. Luo, T. Wang, C. Hong, Y.C. Yang, Y. Chen, Y.J. Cen, S. Q. Xie, C. J. Wang, Eur. J.
Med. Chem., 122 (2016), 17–26.
2. S. Jeelan Basha, Y. Daniel Pushparaju, K.Yelamanda Rao, P. Mohan, Z. Raveendra Babu,
K. Kasi Viswanath, D. Mark Manidhar, A. Dinakara Rao, S. Rajagopal, A. G. Damu,
Bioorganic Chemistry, 88 (2019), 102960.
PP-85: “Synthesis of Arylcyanamides, Aryl Tetrazole Amines,

Benzothiazoles and Benzoxazoles by Copper Catalyzed Desulfurization and
C-N Cross Coupling Strategies”
S N Murthy Boddapati1,2, Hari Babu Bollikolla1⃰

1
Department of Chemistry, Acharya Nagarjuna University, Guntur-522510, A.P., India.
2
Department of Chemistry, Sir C R Reddy College, P G courses, Eluru-534002, A.P., India.
Corresponding author: Dr. B.Hari Babu, Email: dr.b.haribabu@gmail.com
Novel, efficient and simple protocols were developed towards the construction of a
series of aryl cyanamides/aryltetrazoleamines/substituted 2-aminobenzothiazoles and
benzoxazoles from thiourea using an efficient, inexpensive, air-stable and readily available
copper source as catalyst under moderate reaction conditions. The methodologies involve the
copper promoted desulfurization/nucleophilic substitution/electrocyclization and
mono/domino C-N cross coupling strategies. In addition, the reactivity of aryl halides was
studied and the mechanisms have also been investigated. The synthesized compounds were
well characterized by 1H, 13C-NMR and elemental analysis. All the protocols feature for their
operational simplicity and broad substrate scope.
KEYWORDS: Copper catalyst, Desulfurization, Electrocyclization, C-N cross coupling.
(RACPABS - 2020)
PP-86: PRELIMINARY CHEMICAL INVESTIGATION OF PIPER

BETEL LEAVES.
Karanam Santoshi,P Asha Bhanu and G Swapna, Prof.B. kesava Rao,*

Department of Chemistry, University College of Sciences, Acharya Nagarjuna University
Nagarjuna Nagar-522510, Guntur Dt, Andhra Pradesh, India.
Betel leaf is a vine from the family of Piperaceae, mostly consumed in India and Asia
as a ‘Paan’. Betel leaves or Pan Patta are mostly grown in South and South-East Asian
countries basically in India, Pakistan, , Bangladesh, Nepal , Sri Lanka Papua New Guinea etc.
Paan growing needs humid and warm climate and semi shed area with well drained soil.
Betel leaf is an excellent analgesic that offers instant relief from pain. It can be used in
alleviating pain caused due to cuts, bruises, rashes. Betel leaves are a powerhouse of
antioxidants which clear radicals from the body. Leaves have amazing antiseptic properties as
they are rich in polyphenols especially chavicol offering dual protection from germs. It is also
used extensively in treating arthritis and orchitis. The leaf contains allylbenzene compounds
such as hydroxychavicol, chavibetol, chavicol, estragole, eugenol and methyl eugenol.
KEYWORDS: Betel leaf, antioxidants, allylbenzene, hydroxyl chavicol.
References:
[1] S. Chaurasia, G. T. Kulkarni, and L. N. Shetty, “Phytochemical studies and in vitro
cytotoxicity screening of Piper betle leaf (PBL) extract,” Middle-East J. Sci. Res., vol.
6, pp. 532–536, 2010.
[2] P. Guha, “Betel Leaf: The Neglected Green Gold of India,” J. Hum. Ecol., vol. 19, no.
2, pp. 87–93, 2006
PP-87: Design, Synthesis and Anticancer Evaluation of 1,2,3-Triazole

Likned Thiazole-1,2-Isoxazole Derivatives as Anticancer Agents
Thota Yakanthama, Reddymasu Sreenivasulub, and Rudraraju Ramesh Rajua*

a
Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Andhra
Pradesh, 522510, India
b
Nehru Technological University, Kakinada, 533003, Andhra Pradesh, India
*Corresponding author E-mail: rrraju1@gmail.com
A series of novel 1,2,3-triazole likned thiazole-1,2-isoxazole (14a-j) derivatives were

designed and synthesized. Further, all the derivatives were characterized by 1H NMR, 13C
NMR, and Mass spectral analysis. Moreover, these compounds were tested for their
anticancer activity towards MCF-7 (breast cancer), A549 (lung cancer), Colo-205 (colon
(RACPABS - 2020)
cancer) and A2780 (ovarian cancer) four human cancer cell lines by using MTT method. The
well-known anticancer agent etoposide was used as standard reference. The results indicated
that most of the tested compounds were showed good to moderate activity on all cell lines
with IC50 values ranging from 0.01±0.009 to 19.5±5.13 µM, standard drug displayed IC50
values range from 0.13 ± 0.017 to 3.08 ± 0.135 µM. Among them, compounds 14b, 14e, 14g
and 14h showed stronger inhibitory activity than etoposide against four cell lines. In
particularly, one compound 14h was exhibited most promising activity.
Keywords: Triazole, thiazole, isoxazole, MTT assay, anticancer activity.
(RACPABS - 2020)
 PHARMACEUTICAL
PP-1: EVALUATION OF ANTIBACTERIAL ACTIVITY OF SILVER

NANO-PARTICLES ON BANANA BLOSSOMS
B.Nausheen
Department of Nano Science , Prabhath institute of pharmacy , Nandyal, Kurnool dist.
Application of Nano technology is one of the most vigorous research work in present
technological field. Antimicrobial efficiency of banana blossoms with silver nano particles
were examined using methanol and water extract. The micro-organisms such as E.coli,
Bacillus, Streptococcus are used for the anti microbial activity. They are analysed by zone of
inhibition method, disk diffusion method. The formation of silver nanoparticles are
confirmed by FT-IR studies with ultra violet spectro metric analysis showed maximum peak
absorbance was noted at 350-450 nm and also visible by the formation of blackish brown
colour solution of the extract. The macersted extracts of blossoms were tested on micro-
organisms, in which 1mM AgNO3 (std) and 10 mg antibiotic, 10-50 micro gm of plant
methanol extract are selected for the studies. The blossom extracts with nanoparticles show a
high zone of inhibition when compared to normal blossom extract and antibiotic. This
activity supports the use of nano particles in the treatment of resistance bacterias which is
derived from natural extracts of banana
PP-2: IN-SILICO PREDICTION AND MOLECULAR DOCKING

STUDIES AS NOVEL TARGETS FOR VARIOUS DRUGS
Thota Imran Basha
Prabhath institute of pharmacy, Nandyal,Kurnool(dist).
Mail I'd:malliboya6@gmail.com
Discovering drugs to a disease is still a challenging task for medical researchers due
to the complex structures of biomolecules which are responsible for disease such as
AIDS,cancer,autism,alzimer etc. Design and development of new efficient anti drugs for the
disease without any side effects are becoming mandatory in the recent history of human life
cycle due to changes in various factors which includes food habit environmental and
migration in human life style. Cheminformatics deals with discovering drugs based in
modern drug discovery techniques.Che informatics is a new emerging interdisplinary field is
primarly aims to discover novel chemical entities (NCE).Cheminformatics software,
calculations of molecular properties, prediction of bioactivity, virtual screening, molecular
data bases .
(RACPABS - 2020)
PP-3: SYNTHESIS AND EVALUATION OF ISATINS SHOWING ANTI

MICROBIAL ACTIVITY
Bandapalle Malli
Prabhath institute of pharmacy, Nandyal, Kurnool (dist)
with the aim of developing potential anti-microbials a series of novel isatin

derivatives was synthesize in the present investigation. involving reactions of isatin with a
view to synthesized some biological activity compounds. the first isatin derivatives was
synthesized by erdamann and lorentisatin derivatives posses anti bacterial , anti convulsant,
anti depressants, sedatives , hypnotics and anti inflammatory and soon here. we focus on the
isatin showing anti microbial activity.
PP-4: GREEN PROJECT IS BOTH CONVERSATION AND

EDUCATION INTIATIVE FOCUSED ON SUSTAINABLE USE OF
LAND. ONE OF THE GOALS OF GREEN CHEMISTRY IS TO
PREVENT POLLUTION AFTER IT HAS OCCURED.
D.G.Manasa
Prabhath Institute of Pharmacy, Nandyal,kurnool dist.
The remarkable absorption capacity biodegradable and non-toxicity of ligin

nanopartcles enable them to be appropriate vehicles for drug molecules and inorganic
particles. Lignin nano[articcles have porential applications in the biomedical amd
environmental field, such as tissue enginering (or) regeneration artifical muscles, and sstrong
under water antifouling materials. Additionally, the synthesis of nanoparticles using
physicochemical methods can generate toxic and hazardous effecs in the environmenr and
often need expensive materials and instruments. Thus, applications of cost effective, safer,
simple, sustainable and ecofriendlu approaches for fabricating nano particles which comply
with the greenchemistry principles are very important. In this review significant recent
advancement pertaining to the biomedical and therapeutic applications of lignin nanoparticles
are highlighted including their geener synthetic options.
(RACPABS - 2020)
PP-5: poisonous chemicals emitted in factories are dangerous and exposes

us to various elements that cause cancer
S.Vanaja
Prabhath institutions of pharmacy, nandyal, kurnool dist.
Pollution is the introduction of contaminants in to a natural environment that cause

instability, disorder, harm or discomfort to the ecosystem i.e, physical systems or living
organisms. Pollution can take the form of chemical substances or energy, such as noise, heat,
or light. Pollutants, the elements of pollution, can be foriegn substances or energies, or
naturally occuring; when naturally occuring, they are considered contaminants when they
exceed natural levels.
PP-6: BIOANALYTICAL TECHNIQUES FOR DETERMINATION OF

DRUG AND IT’S METABOLITE
M.Balakrishna, Ch.Suneetha and K. Padmalatha

Department of Pharmaceutical Analysis
Vijaya Institute of Pharmaceutical Sciences For Women, Enikepadu, Vijayawada
Bioanalytical method relates specifically to determine the concentration of drug and

its metabolite or both in biological matrix such as plasma, plasma, serum, urine etc. The
purpose is to perform the pharmacokinetics, pharmacodynamic and bioequivalence studies.
Various bioanalytical studies such as hyphenated techniques and ligand binding assays are
important for new drug discovery, new drug development, preformulation studies, analysis of
compound and bioanalytical research purpose. In this article contains complete information
about bioanalytical techniques for determination of drug and its metabolite.
(RACPABS - 2020)
PP-7: COMBINATORIAL CHEMISTRY IN DRUG DISCOVERY: A

REVIEW
DPrasanna, A.Hari chandana and K.Padmalatha

Department of medicinal chemistry
Vijaya institute of pharmaceutical sciences for women, Enikepadu, Vijayawada
Combinatorial chemistry is redefining the way pharmaceuticals and other high

performance chemicals and materials as discovered and developed. Since several
combinatorial chemistry methods have been developed to create diverse chemical libraries
with a wide range of linear or macrocyclic chemical molecules and organic molecules.
Combinatorial chemistry is used because it is affordable, faster, less expensive and more
comprehensive exploitation of new drug targets. As with every new technological
breakthrough, combinatorial chemistry imposes new operational constraints while improving
the efficiency of discovery operations. In this article, we provide a brief overview of
combinatorial chemistry in drug discovery with examples on recently developed new
technologies for design, synthesis, screening and decoding of combinatorial library. The
limitations and strength of combinatorial chemistry are also briefly discussed.
Key Words: Combinatorial chemistry, combinatorial library, drug discovery, comprehensive
molecules, efficacy.
PP-8: THE NEXT LEVEL IN CHEMICAL SPACE NAVIGATION:

GOING FAR BEYOND ENUMERABLE COMPOUND LIBRARIES
D.Prasanna, T.Deepika and K. Padmalatha

Department of Pharmaceutical chemistry
Vijaya Institute of Pharmaceutical Sciences for Women,Enikepadu,Vijayawada-521108,India
Recent innovations have bought pharmacophore driven methods for navigating virtual
chemical spaces, the size of which can reach into the billions of molecules, to the finger tips
of every chemist. There has been a paradigm shift in the underlying computational chemistry
that drives chemical space search applications, incorporating intelligent reaction knowledge,
into their core so that they can readily deliver commercially available molecules as nearest
neighbour hits from within gaint virtual spaces. These vast resources enable medicinal
chemistry to execute rapid scaffold-hopping experiment, rapid hit expansion, and structural
activity relationship, exploitation in largely Intellectual property free territory and at
unparalleled low cost.
(RACPABS - 2020)
PP-9: BIOMARKERS IN ACUTE MYOCARDIAL

INFARCTION:CURRENT PERSPECTIVES
M. Bala Krishna, Divya Sree. Ch and K. Padmalata

Department of Analysis
A bio-marker is a measurable indicator of some biological state or condition.

Biomarkers are often measured and evaluated to examine normal biological processes,
pathogenic processes, or pharmacologic responses to a therapeutic intervention. Acute
myocardial infarction (AMI) is a disease related to heart which is the most common cause of
death in the world. Comprehensive risk assessment of patients presenting with chest pain,
eliminating undesirable results should decrease morbidity and mortality rates, increase the
quality of life of patients, and decrease health expenditure in many countries. In this study,
the advantages and disadvantages of the enzymatic and nonenzymatic biomarkers used in the
diagnosis of patients with AMI are given in historical sequence, and some candidate
biomarkers with a possible role in the diagnosis of AMI are discussed.
PP-10: HERBAL MEDIATED SILVER NANOPARTICLES: A NEW

HORIZON OF ANTINEOPLASTIC DRUG DELIVERY SYSTEM
S.Venkateswara Rao, M.Anvitha*, Padmalatha Kantamneni

Vijaya Institute of Pharmaceutical Sciences for Women, Vijayawada, Andhra Pradesh, India.
Cancer is a disease characterized by the uncontrolled growth and spread of abnormal

cells, and is still the second most common cause of death worldwide. Several classes of drugs
are available to treat different types of cancer. Currently, researchers are paying significant
attention to the development of drugs at the nanoscale level to increase their target specificity
and to reduce their side effects. Silver nanoparticles are the topics of researchers because of
their distinctive properties (e.g., size and shape and electrical properties). Synthesis of herbal
mediated silver nanoparticles targeting biological pathways has become tremendously
prominent due to the higher efficacy and fewer side effects as compared to other commercial
cancer drugs. A variety of preparation techniques have been reported for the synthesis of
silver nanoparticles such as physical, chemical and biological methods. In this review,
different medicinal plants and their active compounds, as well as synthesized silver
nanoparticles from medicinal plants, are discussed in relation to their anticancer activities.
(RACPABS - 2020)
PP-11: ORGAN –ON- A- CHIP TECHNOLOGY: A REVIEW
D.Prasanna, O.KrupaSanthi and K.Padmalatha

Department of Pharmaceutical Chemistry
Organ-on-a-chip is a 3D invitro microfabricated unit that contains a multichannel

microfluidic cell structure. It stimulates the activites, mechanics and physiological response
of entire organs and organ system. This technology is used to develop human organs on chip
such as lung, gut, liver, heart, skin, bone marrow, blood brain barrier, kidney. These micro
devices are translucent and provide a window to see organ’s functionality, behavior and
response at cellular and molecular level. Organ-on-a-chip offer a potential alternative to
traditional animal testing. They mimic the physical microenvironment of organs and human
specific disease state. Organ chips are used as tools for accurately predicting drug efficacies
and toxicity. The use of an organ- on-a-chip model in the drug development process can be
beneficial in either the basic research stage or in the preclinical stage. This could change the
drug development process by replacement of animal models. Thus organ-on-a-chip is an
important tool in new drug discovery process.
PP-12: ROLE OF NATURAL PRODUCTS IN DRUG DISCOVERY
Jayarami Reddy A, Rajyalakshmi K and K.Padmalatha

Department of Pharmacology,
Vijaya Institute of Pharmaceutical Sciences for Women
Enikepadu, Vijayawada, Andhra Pradesh, India.
Drug discovery using natural products is a challenging task for designing new leads. It
describe the bioactive compounds derived from natural resources, its phytochemical analysis,
characterization and pharmacological investigation. It focuses on the success of these
resources in the process of finding and discovering new and effective drug compounds that
can be useful for human resources. From many years, natural products have been acting as a
source of therapeutic agents and have shown beneficial uses. Only natural product drug
discovery plays an important role to develop the scientific evidence of these natural
resources. Research in drug discovery needs to develop robust and viable lead molecules,
which step forward from a screening hit to a drug candidate through structural elucidation
and structure identification through GC–MS, NMR, IR, HPLC, and HPTLC. The
development of new technologies has revolutionized the screening of natural products in
discovering new drugs. Utilizing these technologies gives us an opportunity to perform
(RACPABS - 2020)
research in screening new molecules using a software and database to establish natural
products as a major source for drug discovery. It finally leads to lead structure discovery.
Powerful new technologies are revolutionizing natural herbal drug discovery.
PP-13: PRINCIPLES AND APPLICATIONS OF LIQUID

CHROMATOGRAPHY-MASS SPECTROSCOPY
M.Vamsi Krishna, N.Sailaja and K.Padmalatha

Department of Pharmaceutical Analysis
Liquid chromatography-mass spectroscopy is an important analytical tool .It is a

combined action of both liquid chromatography and mass spectroscopy.It has its use in
various stages in new drug discovery from chemical synthesis to compound selection for
development .There are many types of mass spectrometers available for interfacing with
HPLC .The more common system used for HPLC-MS is single quadrupole mass
spectrometer .An HPLC-MS system will be used to provide information on compound
identity and purity .It has many advantages when compared to normal HPLC that it reduces
the number of samples that had to be injected.LC-MS has also become a common method for
speeding up throughput discovery.LC-MS also found its use in identification of metabolites
.It is also used in chemistry library synthesis and ADME-PK screening (In vitro screening
and In vivo screening). Thus HPLC-MS continue to be an important part of new drug
discovery process. This review article contains complete information about LC-MS.
PP-14: GREEN CHEMISTRY AND ITS MULTIDIMENSONAL

IMPACTS: A REVIEW
D.Prasanna, R .Satyaveni and K. Padmalatha

Department of Pharmaceutical Chemistry
Vijaya Institute of Pharmaceutical Sciences For Women,Enikepadu, Vijayawada
The growing process of industrialization was a milestone for world economic

evolution. Twelve principles of green chemistry rely on the non use of toxic solvents in
chemical processes and the non generation of wastes from these process. The main objective
is to minimize the environmental and occupational hazards in industrial activities. The
impacts of green chemistry are multidimensional. The future of green chemistry as well as
our future and the environment is also contemplated in this work. The impacts of green
chemistry on pharmaceutical analyzers, environmental, population, analyst and company are
described in this review.
(RACPABS - 2020)
PP-15: NANOMEDICINE IN DIABETES MELLITUS- A REVIEW

A Jayarami Reddy, P. SINDHU and K. Padmalata
Department of Pharmacology, Vijaya Institute of Pharmaceutical Sciences for Women
Enikepadu, Vijayawada, Andhra Pradesh, India.
Diabetes is a chronic disease which has been affecting the global population for a long
time. Diabetes mellitus is a growing health problem in the world that causes severe morbidity
and mortality. The prevalence of diabetes is rising day by day. Nanotechnology-based
approaches hold substantial potential for improving the care of patients with diabetes.
Nanoparticles are being developed as imaging contrast agents to assist in the early diagnosis
of type 1 diabetes. Glucose nanosensors are being incorporated in implantable devices that
enable more accurate and patient-friendly real-time tracking of blood glucose levels, and are
also providing the basis for glucose-responsive nanoparticles that better mimic the body’s
physiological needs for insulin. Finally, nanotechnology is being used in non-invasive
approaches to insulin delivery and to engineer more effective vaccine, cell and gene therapies
for type 1 diabetes. Here, we analyse the current state of these approaches and discuss key
issues for their translation to clinical practice.
PP-16:ROLE OF TELOMERES IN CANCER AND AGEING

T. Maniratnam
Department: pharmaceutical chemistry; Prabhath institute of pharmacy, Nandyal, Kurnool dist.
Telomeres are structures found at the end of human chromosomes that contain
thousands of repeats of repetitive TTAGGG DNA sequences which protect genome from
nucleolytic degradation, unnecessary recombination and interchromosomal fusion. Telomeres
therefore play a vital role in preserving the information in genome. As normal cellular
process, a small portion of telomere DNA is lost with each cell division. Telomere length in
humans seem to decrease at a rate of 24.8-27.7 base pairs per year the rate of telomere
shortening can either increase or decrease by specific lifestyle factors. Progressive shortening
of telomeres leads to senescence, apoptosis, or oncogenic transformation of somatic cells,
affecting health and lifespan of individual. Individuals with shorter telomeres seem to have
great risk for development of lung, bladder, gastrointestinal, head and neck cancer, may have
genetic disorder and at a greater risk to develop premature coronary heart disease and
premature aging. An enzyme named telomerase adds bases to the ends of telomeres. It is
present in germline and hematopoietic cells. Telomerase’s are reactivated in most cancers and
immortalized cells. The activation of telomerase represents the most common pathway for
stabilizing telomeres in human cancers. Better choice of diet and activities has great potential
to reduce the rate of telomere shortening or prevent excessive telomere attrition, leading to
delayed onset of age associated disease and increased lifespan.
(RACPABS - 2020)
 BIOLOGICAL
PP-1, EFFECT OF SALT STRESS ON FOXTAIL MILLET (Setaria

italica L.Beauv) DURING SEED GERMINATION
D. Rama Rao and Y.R.K.V.Tirupati Rao*

Department of Botany and Microbiology, Acharya Nagarjuna University
Nagarjuna Nagar – 522510 Guntur, Andhra Pradesh. ramarao472@gmail.com
Salt stress is an important abiotic stress that confers enormous loss to the crops, and
one of the serious limiting factors for the growth and productivity of crop yield in arid and
semi-arid regions. Salt stress is the focus screening of 24 Foxtail millet (Setaria italic L.
Beauv) accessions, procured from ICRISAT. All these seeds were grown in plastic trays
treated with four different salt concentrations, i.e., Control, 60mM, 120mM and 180mM of
NaCl for 9 days old seedlings. The parameters studied include Germination Percentage (%)
Root Length, Shoot Length, Seedling Vigour Index(SVI), Relative Water Content (RWC),
Fresh and dry weigh. It was found in our study that maximum tolerance towards salt stress
was shown by ISE-375 and ISE-783. The increased sensitivity towards salt was exhibited by
accessions ISE-1269 and ISE-963. The remaining accessions were found to be moderately
tolerant of salt stress. So the objective of the present study, screening of 24 different
accessions of foxtail millet seedlings were studied regarding germination and their different
growth parameters.
PP-2:EXTRACTION, IDENTIFICATION AND ESTIMATION OF

THIAMINE IN WHOLE GRAINS BY HPLC
Dr.K.Suneetha and Hari chandana

Department of Biochemistry, Acharya nagarjuna university
Vitamins are a class of essential nutrients in the body, thus, they play important t roles
in human health. Thiamine (B1) is essential to the health of all living organisms and
deficiency has long been associated with diseases in animals such as fish, bird, alligators, and
domesticated ruminant animals. Thiamine deficiency results in the disease called Beri- Beri.
Thiamine functions as the co-enzyme thiamine pyrophosphate (TPP) in the metabolism of
carbohydrates and branched chain amino acids (1).Whole grains or foods made from them
contain all the essential parts and naturally occurring nutrients of the entire grain seed in their
original proportions (2). The whole grains consists of three main parts: Germ (contains the
(RACPABS - 2020)
plant embryo or seed contains genetic material for a new plant, essential fatty acids, vitamin
E, B-group vitamins, minerals, phytonutrients), endosperm (provides food for the growing
seed starchy carbohydrates and protein), outer bran (contains vitamins, minerals and
phytonutrients) along with aleurone layer (located between the endosperm and the bran, but
in the milling process, the aleurone layer is removed together with the bran) (3). The thiamine
rich whole grains are barley, brown rice, whole wheat. The thiamine was extracted by using
boiling water and the identification of thiamine done through Thin layer chromatography (4)
The estimation of thiamine is done through High performance liquid chromatography.
PP-3: IDENTIFICATION AND ESTIMATION OF FLAVONOIDS IN

ALOEVERA BY TLC AND HPLC
Dr.K.Suneetha, R.Sushma and M.Sri Lakshmi Durga

Department of Biochemistry, Acharya Nagarjuna University
Aloevera, a succulent perennial and drought resisting plant, is well known for its
therapeutic potential(1). A number of beneficial effects of Aloevera have been reported,
including immunomodulatory, anti-carcinogenic, wound and burn healing, hypoglycemic,
anticancer, gastro-protective, antifungal, and anti-inflammatory properties(2). Flavonoids are
an important class of natural products; particularly, they belong to a class of plant secondary
metabolites .This kind of plants can be utilized as new sources of natural antioxidants(3).
In this study Aloevera powder has been taken because it is one of the medicinal plant
and it is rich in vitamins, Enzymes, sugars, aminoacids, vitamins-A, C, E and B12. For the
identification of the compounds which is present in the given sample Thin Layer
Chromatography(TLC) method is used, With the help of TLC the flavonoids and phenolics
compounds are identified Rf values are calculated, estimation of compounds can be done with
the help of HPLC.
(RACPABS - 2020)
PP-4, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF

PHYTOCHEMICAL CONSTITUENTS OF ZORNIA DIPHYLLA L.
L. Mutyala Naidu
Department of Botany, Adikavi Nannaya University, Rajamahendravaram-533296,
Address for corresponding: lagudu3@gmail.com, Mobile: 09885361145
Microbial pathogens develop resistance to a particular antibiotic after repeated

administration during the treatment of infectious diseases. Moreover, multiple drug resistance
is a very common problem especially in hospital acquired infections. Therefore, it is
necessary to find out alternative antimicrobial drugs and present trend is focused on
medicinal plants. This preliminary research work was carried out to find out the antimicrobial
and antioxidant activity of solvents as well as aqueous extracts of Zornia diphylla whole
plant. The extracts were tested against bacterial and fungal strains by agar well diffusion
method. It was observed that methanol extract showed the highest zone of inhibition against
Multi drug resistance Staphylococcus aureus at 100 mg/ml concentration. The most
susceptible bacterium was S. aureus. However, Enterococcus faecalis, Micrococcus luteus
and Streptococcus pneumoniae are also susceptible. But the Klebsiella pneumoniae is
resistant against the tested extracts. The extracts contained relatively higher levels of total
phenolics and flavonoids. All the extracts showed dose dependent reducing power activity
and moreover, they are well correlated with the total phenolic substances. A similar dose
dependant trend has also been observed for hydroxyl radical scavenging activity and DPPH
radical scavenging activity.
PP-5:CYANOTOXINNS AND ALGAL-BLOOMS
M. Rajitha, Sudharani Chalapala and S. Anwar

Blooms containing microcystin were creates a problem worldwide in freshwater

ecosystems. Microcystins are cyclic peptides and can be very toxic for plants and animals
including humans. They accumulate in the liver of fish, in the hepatopancreas of mussels, and
in zooplankton. They are hepatotoxic and can cause serious damage to the liver in humans. In
this way they are similar to the nodularins and conbinely the microcystins and nodularins
account for most of the toxic cyanobacterial blooms in fresh and brackish waters. In 2010, a
number of sea otters were poisoned by microcystin. Marine bivalves were the likely source of
hepatotoxic shellfish poisoning. This was the first confirmed example of a marine mammal
dying from ingesting a cyanotoxin.
(RACPABS - 2020)
PP-6:THE GREEN SYNTHESIS, CHARACTERIZATION, AND

EVALUATION OF THE BIOLOGICAL ACTIVITIES OF SILVER
NANOPARTICLES
M. Sandhya Rani, B. Pavani Bai, G. Gowthami, Sudhakar Podha*.

Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna nagar.
Corresponding author Email.id: sudhakarpodha@gmail.com
The advance of reliable and eco-friendly strategies for the development of

nanoparticles is a fundamental key to the discipline of nanotechnology. Nanoparticles have
been continuously evaluated and have been used in many industrial applications for a decade.
The green route of synthesis is rather safe and eco-friendly when compared to physical and
chemical means of synthesis. This is a simple, cost-effective, stable for a long time and
reproducible aqueous synthesis method to obtain a self-assembly Ag nanoparticles. The size
and shape of Ag nanoparticles were characterized by XRD, TEM, and SEM-EDS. The
formation and stability of the reduced silver nanoparticles in the colloidal solution were
monitored by UV-Vis spectrophotometer analysis. Zeta potential was confirmed by DLS
study. Furthermore, XRD analysis confirmed the crystalline nature of the particles. FTIR
analysis was carried out to identify possible biomolecules responsible in reduction of silver
ions. The antibacterial activity of the biosynthesized Ag NPs against Gram-positive and -
negative bacteria species was done by disk diffusion method. Antimicrobial assay verified
bactericidal activity of biosynthesized Ag NPs against Gram-positive and Gram-negative
bacteria. According to the results, by growing the plants under controlled conditions, it is
feasible to synthesize nanoparticles with desired properties. The powerful bioactivity
demonstrated by the synthesized silver nanoparticles leads towards the clinical use as
antibacterial, antioxidant as well as cytotoxic agent.
PP-7: Β-SITOSTEROL (BS) BIOLOGICAL ACTIVITY

R. Gayathri, Sudharani Chalapala and S. Anwar
Activation of IR and GLUT4 in the adipose tissue of high fat and sucrose-induced
type-2 diabetic patients. The aim of the present topic was to evaluate the antioxidant potential
of β-sitosterol (BS), a phytosterol from Abutilon indicum, using an experimental model for
diabetes-induced oxidative damage. Treatment with BS doses also increased pancreatic
antioxidant levels, with a concomitant decrease in thiobarbutiric acid-reactive substances.β-
Sitosterol has promising antidiabetic as well as antioxidant effects and may be considered in
clinical studies for drug development.
(RACPABS - 2020)
Abutilon indicum
Beta- Sitosterol
PP-8: ENVIRONMENTAL BIODIVERSITY CONSERVATION
S. Rehana, Sudharani Chalapala and S. Anwar

The term bio diversity was 1st coined by WALTER G.ROSEN in 1986.The biosphere
comprises of a complex collection of innumerable organisms known as biodiversity, which
constitute the vital life support for survival of human race. Bio diversity is the variety of flora
and fauna on this planet earth.
(RACPABS - 2020)
Conservation of biological diversity leads to conservation of essential ecological

diversity to preserve the continuity of food chains. Conservation of biodiversity can lead to
health risks because of the positive relationship between biodiversity and diversity of
infectious diseases.
PP-9:MARINE FUNGI - A SOURCE OF BIOACTIVE METABOLITES
S. Shabana1, k. Rajya Lakshmi1 and A. Krishna Satya1*

1. Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar-522510,
Marine fungi are potent sources of bioactive compounds for medicinal and
pharmaceutical industries. Bioactive metabolites such as polyketides, sesquiterpenoids,
alkaloids, diterpenoids, indole derivatives, ether derivatives, peptides and azaphilone
derivatives are reported from marine fungi. So, in order to find the novel bioactive
compounds from marine fungi we have selected the Bay of Bengal coast. Further, this present
communication is on the marine fungi of the Hamsaladeevi (Bay of Bengal coast), Krishna
district, Andhra Pradesh, India. In this report, 5 species of marine fungi belonging 3 genera
are reported with their habitats. Interestingly some Penicillium sps are new to this area.
Intensive work is in progress to study the rest of the marine fungi from this locality, as well
as to screen the bioactive metabolites.
PP-10: COMPARATIVE STUDIES ON THE BIOCHEMICAL COMPOSITION OF

MALE AND FEMALE MUD CRABS, SCYLLA SERRATA AND S. OLIVACEA
Parvathi, D. and P. Padmavathi

Department of Zoology and Aquaculture
Acharya Nagarjuna University, Nagarjuna Nagar – 522 510 Andhra Pradesh, India
Email:sharonblessy@gmail.com and padmapin@yahoo.com
The biochemical composition (glycogen, proteins, fat, ash and moisture) of muscle
tissue in male and female mud crabs Scylla serrata and S. olivacea was determined. Twelve
samples collected from Scylla serrata and S.olivacea male and females (1:1) were used in this
study. The mean glycogen, protein and fat contents are significantly (p<0.05) higher in
females than in males of both species. In Scylla serrata, the biochemical composition of male
and female crabs respectively are 3.943±2.091, 4.593 1.249 of glycogen; 64.17±9.370,
70.503±14.279 of protein; 2.583±0.687, 3.043±0.853 of fat; 10.793±3.060, 7.063±0.99 of
(RACPABS - 2020)
ash; and 81.033±1.296, 80.91±4.718 of moisture, whereas in S. olivacea, the respective

contents in male and female crabs are 4.116±2.851, 5.156±2.532 of glycogen, 62.29±14.039,
66.006±16.959 of protein, 3.106±1.091, 3.476±0.863 of fat, 10.303±3.961, 8.436±3.127 of
ash and 80.47±2.215 of moisture. The results indicated that there are marked variations in the
biochemical components of male and female crabs of two species and the females are
nutritionally superior over male crabs.
PP-11:EFFECTS OF PESTICIDAL POLLUTION ON ENVIRONMENT
K. Ravibabu, G.Rajeswari& Dr. V. VenkataRathnamma

Department Of Zoology & Aquaculture
Acharya Nagarjuna University, Guntur – 522510. A.P, India
Pesticide are chemicals used to kill different kinds of pests which are harmful and
develops a decrement in concern agricultural production. Depending upon the concentration
of pesticides, the exposure of pesticides are two types. Long term pesticide exposure has been
linked to development of Parkinson disease,asthmaand anxiety,attention deficit and
hyperactivity disorder {ADHD}. Short term exposure includes skin rashes, eye irritation and
lethal effects might occur. Man-made toxic chemicals are released into the environment
during production, transportation as well as utilization, and thus pose a threat to living biota.
Therefore the assessment of environmental hazards due to toxic substances is an important
challenge to toxicologists and Eco toxicologists. Pollution is the unfavorable alteration of our
environment, largely because of human activities. Pollutants are substances, which cause
pollution. They might the substances that occur in nature or unnatural substances released
into the environment by human handiwork- ego pesticides, herbicides etc. More pesticides
have been developed and spread across both agricultural and urban landscapes. Thus, it is no
wonder that pesticides or the residues of degraded residue pollute much of our water
resources. The pollution of water resources by (1) the direct application of pesticides to water
bodies to control aquatic plants (2) the indirect pollution of surface waters by air transport
and/or surface water runoff of pesticides and (3) the pollution of groundwater by pesticide
seepage through soil has been well documented since the 1940s.One of the best ways to
remove 97-99% of all pesticides ,insecticides and herbicides from drinking water with a
reverse osmosis or granulated activated carbon system. Buying of organicand locally grown
fruit and vegetables to escape from contaminated of pesticides.
(RACPABS - 2020)
PP-12: NUTRITIONAL GENOMICS: AN OVERVIEW
S. Sundar, S. Geetha and K.Padmalatha

Department of Biotechnology
Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu, vijayawada
Nutritional genomics are also known as nutrigenomics. Nutritionalgenomics is the

combination of two areas nutrigenomics and nutrigenetics. Nutrigenomics is the study of how
foods influence gene expression and how individual genetic variation affects the way an
individual responds to nutrients in food. Bioactive food components are nutrients and
phytochemicals of foods that alter physiological traits by interacting directly or indirectly
with genes. Nutrigenomics will determine the association between diet and chronic diseases
which will help to understand the etiologic aspects of chronic diseases such as cancer, type-2
diabetes, obesity and cardiovascular disease (CVS). In this article, complete information
about the nutrients and related diseases are discussed.
Keywords: Genomics, nutritional, cancer, obesity.
PP-13:AMINOACIDS FOR PLANTS
M.Kala, Sudharani Chalapala and S. Anwar

Proteins have a structural function, metabolic function (enzymes), a transport function

and a stock of Amino Acids function. Only L - Amino Acids are assimilated by plants. ...
These Amino Acids help to increase chlorophyll concentration in the plant leading to higher
degree of photosynthesis. Plants are a rich source of amino acids and their individual
abundance in plants is of great significance especially in terms of food. Therefore, it is of
utmost necessity to create a database of the relative amino acid contents in plants as reported
in literature. Since in most of the cases complete analysis of profiles of amino acids in plants
was not reported, the units used and the methods applied and the plant parts used were
different, amino acid contents were converted into relative units with respect to lysine for
statistical analysis.The most abundant amino acids in plants are glutamic acid and
aspartic acid. Glycine and Glutamic Acid are fundamental metabolites in the process of
formation of vegetable tissue and chlorophyll synthesis. These Amino Acids help to increase
chlorophyll concentration in the plant leading to higher degree of photosynthesis. This makes
crops lush Green.
(RACPABS - 2020)
PP-14:REDUCINGTHE INCIDENCE OF INSULIN RESISTANCE BY

THE MUTAGNESIS OF WHEAT
S. Sundar, SupriyaMareedu and K.Padmalatha

Department of Biotechnology
Vijaya Institute of Pharmaceutical Sciences for Women, Enikepadu, Vijayawada.
Wheat is one of the mostly used food supplements in many nations across the globe.
The incidence of diabetes is gradually increasing day-by-day. One of the epidemiological
factor associated with the development of insulin resistance in adults as well as in elderly
people is consumption of Triticum aestivum(wheat). This is also a factor influencing the
development of type-2 diabetes. The known cause for the occurrence of celiac disease is the
gluten protein which is a major constitute of the wheat.So,by the mutagenesis of the Triticum
aestivum at the particular sites leads to the development of new variety. This mutagenesis can
be done with the new DNA editing technologies like CRISPR,etc.By this, adverse effects on
diabetes mellitus type-2, celiac disease by the consumption of wheat can be restricted.As
protein intake enriches their health;especially it serves a lot in the diabetic care of the
patients. In this review article, includes the editing of genome of the wheat brings the
acceptable variety with negligible adverse reaction to the body by it’s consumption.
(RACPABS - 2020)
PP-15: FUNGAL PATHOGEN ASSOCIATED WITH MURRAYA

KOENIGII L.
1
1
Department of Biotechnology, Government Womens College, Kolar-563101, Karnataka.
2
Vikram B.Ed,. College, Chintamani,Kanataka.
Curry leaf (Murraya koenigii L.) of the family Rutaceae have long been considered a
premier leafy vegetable with attractive habit and graceful leaves. It is used in fresh, dried and
processed forms for flavoring food stuffs. The species is native to India and Srilanka. It is
extensively used in the indigenous system of medicine as an anti-diabetic agent. Further it is
known to possess anti-inflammatory, antioxidant and diverse pharmacological properties. The
plant is grown presently in the backyards of every South Indian home, but its nutritive value
and medicinal properties have not been fully appreciated. A very common disease in the plant
is leaf spot and powdery mildew so it has gained least attention of researchers. Curry leaf
plants with leaf spot and powdery mildew disease were collected from Mandya district of
Karnataka, India. The pathogen was isolated on Potato Dextrose agar (PDA) from surface
sterilized small pieces of the leaves and twigs, incubated at 25°C, and identified as Rhizopus
and Aspergillus was the cause for the disease according to its morphological and cultural
characteristics and study was done based on colony characters.
PP-16:ISOLATION AND PURIFICATION OF BIOACTIVE FORM

MICROBES TO ESTABLISH THEIR ANTAGONISTIC ACTIVITY
AGAINST FUNGAL PATHOGENS ON SOYA BEAN.
Qasim Turki Kadhim, Merakanapalli Kishore Babu, Kunchala Rajyalakashmi, DSD

Suman Joshi, Subramaniam Gopalakrishnan, Vadlamudi Srinivas,
Alapati Krishna Satya *
Department of biotechnology, Acharya Nagarjuna University, Nagarjuna nagar, Guntur,
Andhrapradesh-522510, India.
Corresponding author e-mail:akrishnasatya78@gmail.com.
Black gram (Vigna Mungo L.; Family: Fabaceae) is cultivated in the country form
way back 2200 B.C. Guntur District ranks first in Andhra Pradesh for the production of black
gram. Till date chemical pesticides are commonly used to control the effect of pathogens.
Biopesticides or biological pesticides are better alternatives which are eco-friendly and
(RACPABS - 2020)
equally effective. Black gram is commonly infected by fungal pathogens like : Anthracnose:
Colletotrichum lindemuthianum, Bacterial Leaf Blight: Xanthomonasphaseoli, Cercospora
leaf spot: Cercospora canescens, Powdery Mildew: Erysiphe polygoni, Root Rot and Leaf
Blight: Rhizoctonia solani, Rust: Uromyces phaseoli, Macrophomina blight: Macrophomina
phaseolina, wilt: fusarium oxysparum by which the yield is reduced or becomes totally zero
in worst cases. The vermicompost is excellent rich sources of organic pesticide. The main
objective of the present study is isolation, purification, and characterization of antifungal
compounds from actinomycetes of soil samples and vermicompost. Initially samples were
collected from 5 different places in Guntur districts and surrounding area under aseptic
conditions. Actinomycetes and bacteria were isolated fallowing the serial dilution by using
selective media-Actinomycetes isolation media, Nutrient agar media, Potato dextrose agar
media based on pour plate method. Totally 218 micro organism both actinomycetes and
bacteria isolated were tested for biochemical and morphology studies. The best actinomycetes
and bacteria were further analysed for PGP quantitative and qualitative assays such as
phosphatase, cellulase, lipase, protease, chitinase, IAA, Sideophore assays. The best
performed 124 isolates were further to be screened for antibiotic and antagonistic activity
against important fungal pathogens of black gram. Molecular identification, secondary
metabolites extraction, finalised best actinomycetes and bacteria isolates were evaluated in
field trails against the growth of toxic fungal pathogens.
PP-17: IN SILICO STUDIES IN IDENTIFICATION OF MAP4K4

INHIBITORS
Dorka Vijayakumari Beri1,2, Krupanidhi Srirama1

1.
Dept. of Biotechnology, VFSTR Deemed to be University, Guntur, Andhra Pradesh, 522213
2
Govt. College for Women (A), Sambasiva Pet, Guntur, Andhra Pradesh 522001
Mitogen-activated protein 4 kinase 4 (MAP4K4) is a serine/threonine kinase involved

in various signaling pathways like cell activation, differentiation, proliferation and apoptosis.
Over-expression of MAP4K4 plays an important role in different cancers and became an
attractive therapeutic target for cancer (Wright et al., 2003; Collins et al., 2006; Liu et al.,
2011; Liang et al., 2008; Qiu et al., 2012;). The pyridopyrimidine-based analogs as novel
MAP4K4 inhibitors have been performed by using the QSAR model generation program
using ‘Ezqsar’(Shamsara, 2017) . Total 26 molecules having wide range of activities were
taken (Crawford et al., 2014), which were divided into training set and test set. They were
used to build the QSAR model and to validate the model. The best QSAR model generated
from the training set has the regression square value (r2) 0.92, and the validation (q2) has the
value of 0.84. External set of molecules were taken from GSK-PKIS database and were used
(RACPABS - 2020)
to predict the activity using the QSAR model. Out of many (367), two molecules found to
have good predicted inhibitory values. These analogs of the pyridopyrimidine were docked
into the 3D model of the MAP4K4 kinase domain using Autodock 4.2 software (Morris et al.,
2009) and found to have good binding affinity with the enzyme.
PP-18: Studies on Improvement of Efficacy of Lactobacillus acidophilus for

Industrial Productivity
J. Someswari Devi, G. Naveena, K. Jamalaiah, Dr. Alapati Krishna Satya*

Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur,
Andhra Pradesh -522510, India.
Corresponding author e-mail: akrishnasatya78@gmail.com.
The gut microbiome is not a silent ecosystem but exerts several physiological and
immunological functions. For many decades, lactobacilli have been used as an effective
therapy for treatment of several pathological conditions displaying an overall positive safety
profile. Applications of lactobacilli include kidney support for renal insufficiency, pancreas
health, management of metabolic imbalance, and cancer treatment and prevention. In vitro
and in vivo investigations have shown that prolonged lactobacilli administration induces
qualitative and quantitative modifications in the human gastrointestinal microbial ecosystem
with encouraging perspectives in counteracting pathology-associated physiological and
immunological changes. Finally, we need to determine the adequate amount of bacteria to be
delivered in order to achieve the best clinical efficacy decreasing the risk of side effects. Our
main objective in present study is to reduce the cell size of L.acidophilus which results in
increase of spore count thereby enhancing the productivity in industrial sectors. In order to
get short morphology and high cell densities, the protein source was changed with fixed
carbon source (dextrose) from original MRS composition. The peptone was replaced by soya-
peptone, commercial and Hi Media make. Our results shown that Cells grown in
combinations of Hi Media soy-peptone with dextrose (15/15; 20/10) lowered pH 4.0 and OD
600 to ≈1.8. All results are preliminary up to 10lts, 50 lt’s and small fermentors need scale up
and CFU/ml analysis to establish final composition of mediaCommercial and CSL showed
promising results as compared with all. The cell size observed with above combinations was
50% less compared to rest.
(RACPABS - 2020)
PP-19: STUDY OF MUNICIPAL SOLID WASTAGE MANAGEMENT

SCENARIO OF TENALI CITY
B.Satish Babu1, G. Vijaya Kumar 2, Dr.P. Brahmaji Rao3

1&2
Research Scholar, Dept.of Environmental Sciences, Acharya Nagarjuna University.
3
Associate Professor, Dept.of Environmental Sciences, Acharya Nagarjuna University.
Solid waste is the major pollution from the cities in present days, the management of
solid waste is very important in the environment, if we do not follow proper management
methods that may cause several environmental problems. Tenali city is one of the fastest
developing cities in Andhra Pradesh with population of 164.937 (one lakh sixty four thousand
nine hundred and seven) in 2011 in urban agglomeration, registering a growth of 3% the fast
decade. The city has around 43,593 houses which generate 130 metric tons of solid waste per
day. At present they are not participating any scientific processing and disposal of solid
waste. The solid waste collected is being transported to the cheedelapora disposal site where
is being midiscriminately.The Pollution Control Board (PCB) introduced common facilitator
– EVB Technologies in 2005 to collect bio-medical waste from 100 hospitals in Guntur
district out of which 35 are Tenali city (Annual Report CBWTF, A.P.PCB, 2010). Bio-
medical waste dumped by clinics, hospitals and medical labs and its dump the waste
materials in the drains or near municipal garbage collection point.
Key Words: Solid waste, Management, Municipal Corporation, biomedical, Ground water.
(RACPABS - 2020)
(RACPABS - 2020)

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1st INTERNATIONAL

5th to 7th March, 2020

The faculty members Prof.N.S.Prakasa Rao, Prof.P.V.V.Satyanarayana,

At present 7 major research projects are in succession and 10 BSR fellowships, 6

The department of chemistry is offering four specializations: Analytical, Inorganic ,

S.No Title of the Paper and Author Name P.No.

Influence Of Ligand On The Activity Of Metal Ions In Heterogeneous Water-Oxidation 60

Efficient Synthesis of 8-fluoro-10-methyl-3,4-dihydrobenzo[b][1,6] naphthyridine-2(1H)- 61

Molecules with Celebrity Status

Nature, through biosynthetic pathways, generates simple to architecturally very

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 1

IT1: Phytonanotechnology – Anti-diabetic drugs and Possible Mechanisms

Pasupuleti Visweswara Rao

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 2

IT2: Friccohesity and Survismeter: Theory and Applications in Areas of

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 3

IT3: Borneon Natural Products: Anti-Inflammation/Anti-Cancer Activities

Charles Santhanaraju Vairappan

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 4

IT4: WETLAND DEGRADATION AND ITS IMPACTS ON NATURAL

Lakew Wondimu Abachiri

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 5

IT5: Exploring the Prevalence and Risk Factors of Prediabetes in Adult

Dr. R.Vinodhini1*and Dr. Gudeta Imana2

Diabetes mellitus (T2DM) is a state of chronic hyperglycemia due to complete or

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 6

IT6: Recent Approaches in Natural Products for New Drug Development

Dr. K. Suresh Babu

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 7

IT7: Exploring Synthetic Molecules and the Nano-Bio Platform as

The prevalence of drug-resistant pathogenic bacteria in conjunction with an acute

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 8

IT8: Synthesis, spectroscopic and electrical characteristics of alkali

P. Raghava Rao1, A. Siva Sesha Reddy2 and N. Veeraiah2*

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 9

IT9: Synthesis of an antipsychotic drug substance Clozapine with a very

An economic, commercial manufacturing process for clozapine is reported by using

Graphical abstract / Route of Synthesis

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 10

IT10: Design and Synthesis of Novel Bio-active Organophosphorus

Suresh Reddy Cirandur

In the last decades, organophosphorus compounds (OPC’s) have been widely

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 11

IT11: MULTI-FUNCTIONAL LIPID-DYE- Fe@CNPs CONJUGATES

Mandava V. Basaveswara Rao

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 12

IT12: Mutagenesis aids in studying pathogenesis and vaccine development

Ehrlichia chaffeensis is an obligate intracellular tick-borne bacterium that causes

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 13

IT13: Nanoscience in Interventional oncology: Opportunities

Recent developments in nanoscience started to answer outstanding clinical questions

IT14: Green chemistry: Examples and perspectives for future

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 14

Presently, most of the chemicals are synthesized from non-renewable petroleum

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 15

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 16

IT15: Recent Advances in Hydrogen Fuel Technology

Mannam Krishna Murthy

IT16: STRATEGIES FOR PROTECTING CHEMICAL,

Togapur Pavan Kumar

Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar-522510, Guntur, A.P. 17

IT17: Catalysis Using Functionalized Nanomaterials for the Synthesis of

Dr. Kalluri V.S. Ranganath

5-hydroxymethylfurfural (HMF) has been widely recognized as a key intermediate in

D.Prasannaa, S. Ratna Kumaria, Ch. Rajinia, K.Krishnadevi, V.Anuradhaa,c