STEROCLAVE 24 & 55 User’s manual rev.

03/2002 page 1

STEROCLAVE 24 & 55
Sterilisation Autoclaves

USER’S MANUAL

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 2

 IMPORTANT FOR YOUR SAFETY

This user’s manual provides information on the correct
use of the Autoclave. During sterilisation, the pressure
applied to the lid inside the chamber at above 121 °C is
equivalent to 2.1 bars. In order to achieve complete
sterilisation, high temperature and extreme pressure of
saturated steam are essential. The Autoclave must be
handled with great care, then
is strongly recommended to read it
carefully before using this autoclave.

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 STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 3

Dear Customer
We thank you to have chosen once again one product of ours.
We kindly please you to send back us this Warranty Certificate
properly fulfilled.

------------------------------------------------------------------------------

Warranty Certificate
No. _________

Purchasing date _____________________________________

Product code 745 _____

Serial number ________________ Lot No._______________

REGISTRATION DATA OF THE CUSTOMER

Corporation Name __________________________________

Address __________________________________________

CAP ___________________ City ____________________

Contact ________________ Phone __________________

See conditions on page 20

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 4

Application

The Autoclave is used for sterilisation of utensils, glassware, culture

media and solutions in unsealed bottles, All major functions are
controlled by the microprocessor, offering greater safety, reliability
and simplicity-of-use.

Technical specifications 1

Steroclave 24 Steroclave 55

Capacity 23,7 litres 55 litres

Sterilisation vessel Stainless steel AISI 316 Stainless steel AISI 316
Number of programs 20 20
Sterilisation temperature 101 -134 °C 101 -134 °C
Max pressure 2.1 bars at 134 °C 2.1 bars at 134 °C
Sterilisation time 1 - 99 minutes 1 - 99 minutes
Consumption 1.800 VA 2.000
Dimensions (l x d x h) 46 x 52 x 1.03 cm 55 x72 x 1.11 cm
Supply, single phase 230 V, 50/60 Hz 230 V, 50/60 Hz
Weight 67 Kg 85 Kg

1
Subject to variations without previous notice

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 5

Installation

The hood is packed in a carton. Before open it check any sings of

damage on the package. If any damaged is found everything to the
carrier and to your supplier. Neither the manufacturer nor its agent
can be considered responsible for damage in transit, but they will
make any effort to help you claim from the carrier. After this initial
inspection open carefully the original package, in which the
Autoclave has been delivered to you and verify that inside have been
put the following items:

• Autoclave Steroclave
• Perforated pan (inside the pressure container)
• A heat polypropylene tank for receiving hot air/steam exhaust,
preventing leakage of hot steam and bad odours the room

In case you will find that one or more items are missing, please relate
it to us together with the Warranty certificate (see on page 3). We will
provide to deliver you them as soon as possible

Place the unit far away from any heat-source and excessive humidity,
taking care of leaving it at 10 cm of space from the lateral walls.
Insert the electrical plug, after having checked that the characteristics
of the power source suit the ones on the label that is placed next to
the feeding cord.

NOTE
Be sure that the electrical equipment is ground provided and
also that the earth connection is working.

Do not use any extensions or multiple shunts to feed the

instrument

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 6

Description of the working cycle

The Steroclave carries out a sterilisation cycle consisting of:

1. Preheating
Heating of water from the starting temperature of 100 °C value.
2. Air hole (Breather)
Generation of steam, adequate to let the part of the air, still inside the
container, come out from the breather valve.
3. Heating
Warming up of the internal steam up to the value of the set-up
temperature.
4. Sterilisation
Maintenance of the set-up temperature for the fixed time of the
sterilisation.
5. Ending of the Cycle
Acoustic alarm until the intervention of the Operator.

Working preparation

A. Turn the lid handles counter-clockwise and swing the lid

sideways

B. Pour water into chamber

C. Place objects to be sterilised into the chamber. Close the lid.

D. Switch on the unit

E. Select the proper sterilisation program to be executed. If it is not

present, program it as described below.

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 7

Programming

All the functions of insertion or request of numerical data are shown

on the alpha–numerical display, Fig.1 (4) by the symbol > on the left
side of the field, to be inserted or corrected,
Generally a value in the inserting field is proposed to be accepted or
corrected, as you want. The C button cancels all the present numbers
one by one, starting from the one on the far right (last Entry).
The E button confirms the value shown on the display.

Example: Presented value 1.246, desired value 1.346

Push “C” button, it’s shown > 124
Push “C” button, it’s shown > 12
Push “C” button, it’s shown > 1
Push “3” button, it’s shown > 13
Push “4” button, it’s shown > 134
Push “6” button, it’s shown > 1.346

Confirm the shown value by pushing the E button.

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 8

Fig.1

Push the button PROGRAM, Fig. 1, and set up, through the
numerical buttons the number of the program you are going to insert
or modify.

When the number of the program is confirmed, the “STER:TEMP”.

parameter is shown, related to the temperature of the required
sterilisation, which can be modified, as you desire within the working
limitation of the Autoclave (101 °C - 134 °C).

At this point when also this last value is set-up, the parameter
“TIME” is shown, related to the duration in minutes (1-99) of the
maintenance of the sterilising temperature.

Once this last parameter has been inserted, the program is

permanently kept in memory until the following alteration, even in
absence of electrical feeding.

When is necessary, repeat the same operation for the other programs.

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 9

Use

Turn the Steroclave on, by operating on the main switch (located on

the right side of the Control Panel, Fig. 1 (2) of the cover and lift it
up.

If the Autoclave is equipped with the security cap (code 750-CP),

push the appropriate releasing push-button Fig. 2 (1).

Fig. 2

Open the cap of the sterilisation chamber Fig. 2 (3) and turn counter-
wise the closing hand-wheel, Fig. 2 (1), and put the cap itself apart.
Fill the bottom part of the container with a quantity of distilled water,
sufficient to cover the warming worm wheel, placed on the bottom
part. Generally it requires around 2 litres.

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 10

Using water not distilled will cause the formation of lime deposits,
which may damage all the components and the container itself.

Put the perforated pan over the warming worm wheel.

Take the pressure cover back to the closing position, Fig. 2 (2) and
turn the closing hand –wheel clockwise to lock the cover itself.

Verify the following conditions:

• The bottle for the steam harvesting contains at least an half

litre of water
• The steam waste-pipe is plunged in the water itself
• The cap is well screwed down on the bottle
• The hole over the cap, to equalise the pressure, is not obstructed.

If the Autoclave is equipped with the security cover (code 750-CP),

pull it down, then press the appropriate locking push-button.

Verify that the tap is turned off, Fig. 3.

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 11

Fig. 3

Selection of the program to use

Press the button “SELECT”, Fig. 1, and set up, through the
numerical keys, the number of the program you need to use. Press the
button “START” .If the Autoclave is equipped with the security cap
(cover) (code 750-CP), and if it is not properly closed, the message
“CLOSE THE COVER” is shown, when closed the Steroclave itself
is providing to lock it.

On this first step the message “PRE HEATING” will appear till the
displayed temperature is of 100.

During these first minutes of the cycle (on condition that the
Steroclave is “cold”) can be noticed the running of the heating
element and if it is not revealed a proper increase of the temperature,
can be signalised a possible failure in the system and the message
“ERROR 2 NO HEATING” is shown.

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 12

A further check over the heating element locks the cycle in case of
overheating of the element itself, situation marked by an acoustic
alarm and by the message “ERROR 1 OVER HEATING” on the
display.

WARNING
The cap reaches a very high temperature; please pay
attention in touching this part!!!

When the Steroclave reaches the 100 °C it will proceed automatically

to the expulsion of the residual and unsaturable steam and air exhaust
(AIR EXHAUST) still existing in the sterilisation chamber.
The phase of the expulsion of air/steam begins at the same time of the
preheating of the Autoclave and becomes more and more evident
with the raising of the temperature followed in the latest phases, by a
light noise arising from the air and the steam gurgling together
(through the steam recovery bottle.)
At the end of the expulsion cycle the breather valve will
automatically be closed, the message “STEAM GENERATION”
will be on and the temperature will raise till getting the value already
set-up in the program. Once the value has been reached, the message
“STERILISATION” is shown and will start the countdown of the
required time for the sterilisation.
The temperature appearing on the display will be approximated to the
whole closer to it, this means that the changes of the phases due to
the temperature are not in accordance to the visualised value.

When the sterilisation cycle is over, the message “END OF

CYCLE” is shown and you will hear an acoustic alarm till the
operator pushes the button STOP in Fig. 1.

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 13

Right now the program is over and it is time to wait for the
Steroclave to cool down.

It is possible to accelerate this phase operating on the tap, placed on

the right side of the manometer, Fig. 3 (1), turning it counter-wise to
let the steam coming out from the chamber, through the steam
recovery bottle.

It is also possible to use the electrical control by opening and closing

for a short time the breather valve, in case the pressure is already
considerably reduced.

WARNING
An extreme care has to be taken in dosing the entity of the steam-
outlet, as inside the Chamber is existing pressure steam.

If the Autoclave is equipped with the security cap (code 750-

CP), and it is required to unlock it, on the screen will appear
the message “CHECK TEMP.PRESSURE “.

WARNING
Before opening the cap, please verify also that there is not any residual
pressure inside the chamber, watching the indications on the manometer,
Fig. 2 (2) and operating on the control of the air breather valve taking it in
position OPEN. Then proceed anyway with extreme care, reminding that the
machine has reached a very high temperature and because inside of it there is
still steam.

In case you have been waiting until the Steroclave has become cool
without any steam-outlet, it is important to remember that an internal
temperature lower than 100 °C has made a certain gap, which besides
hampering the opening of the cover, can also recall water from the

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 14

exhaust bottle, if there is (as it should be) and the connection through
the tap or the electrical valve is opened.
In that case it is better to remove the exhaust pipe from the bottle
before proceeding, taking care to put it back correctly on position
before using it again.
At the end of the use, take the residual water still inside the
Autoclave away. Verify periodically the status of the heating element,
accessible by taking the perforated pan away, located on the bottom
of the pressure container.
In case of lime deposits, provide to remove them without any damage
for the element itself.

Further information

On the bottom of the Autoclave there is a lever tap to make the

pressure container empty.

Safety devices

Besides the already mentioned checks right inside the sterilisation

cycle, concerning the warming element, there is a further protection
against the overpressure, totally independent from the microprocessor
cycle.

The Printer-recorder

The Steroclave has a standard Printer-recorder to print the report of

the sterilisation cycle.

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 15

Fig. 4

For each executed sterilisation the printer prints as shown in the Fig.
4

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 16

NOTE: F2 key enables or disenables the graphic of the printer

Referring to the Fig. 4 we can note:

1. Graph of the temperature

2. Line indicating the present setpoint
3. Moment of air exhaust
4. Sterilisation period
5. Number and parameters of the program in force
6. Scale of the times, with graduation in minutes (6 values every
minute)
7. Marker of the times and temperatures with a point every 10 °C,
printed every 5 minutes
8. Last data of the cycle

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 17

Fig. 5

If there are events that forced the cycle break the printing reports the
event, Fig. 5.

In case of main power blackout during the cycle, the printer reports
the blackout and restart times.

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 18

Spare-parts list Stero Clave 24

745024102 KT-AUT Electronic Board

745024110 Temperature probe
745024111 0.5% PT100 Validation Probe
745024122 25A Solid state relay
745024130 AC Filter
745024140 Fuse Holder (1 piece)
745024141 10A F.I. Fuse
745024180 80x80mm Axial Fan
745024300 Main switch
745024310 Safety pressure limit switch
745024311 Safety thermostat
745024330 Heating element for 745 024
745024350 Power step-down transformer
745024501 Electro valve for steam
745024502 24VCC Coil for electric valve
745024511 Pressure gauge
745024550 Safety valve for max pressure
745024560 2L Condensed steam tank
745024561 Steam discharge tap
745024600 Sterilisation tank exhausting tap
745024656 Autoclave cover’s gasket
745024850 Validation’s probe port
745024851 Bottom grill for basket
745024CT1 220 x 215 mm (diam. x h) stainless steel basket
745024CT2 220 x 430 mm (diam. x h) stainless steel basket

Spare-parts list Stero Clave 55

The same as the Stero Clave 24, change only the code 024 into 055.

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STEROCLAVE 24 & 55 User’s manual rev. 03/2002 page 19

GUARANTEE CONDITIONS

All our apparatus have been carefully tested and are covered by 12 months
guarantee from the purchasing date.
TEKNOLABO A.S.S.I. S.R.L. undertakes to free repair or replace the parts that
within the guarantee period Teknolabo itself should consider not efficient.
The complete replacement of the apparatus and the extension of the guarantee after
an operation due to a failure are excluded.
TEKNOLABO A.S.S.I. does not respond for possible direct or indirect damages
caused to people or things by failures of the apparatus or depending on forced
suspension of its use.
The guarantee does not cover: aesthetic parts, damages caused by careless, bad or
not proper use or installing, wrong connection to tension different from the proper
one of the apparatus, or different from the limit fixed by CEI rules (+-10% of
nominal value).
The eventual laboratory repairing is performed in our Technical Assistance Centre
in Milan Via Varalli 1/B 20089 Rozzano (Italy), except for different EXPLICIT
indication or authorisation of the manufacturer.
The guarantee declines if the apparatus has been tampered or repaired by not
authorised staff.
The apparatus considered defective will have to be sent to us together with a copy
of the certificate of guarantee.
The expenses and the risks of travelling from and to our laboratory will be at charge
of customer.
In case of field repairing (to be performed only on manufacturer's not discussible
decision), the customer has to pay the calling-right as refund for travelling of the
staff.

Validity of the warranty

The guarantee is valid only if the coupon has been completely fulfilled and sent to
Teknolabo A.S.S.I. S.R.L. within 8 days from the date of receiving the apparatus.
For any dispute should occur the Court of Milan has to be considered competent.

TEKNOLABO A.S.S.I. S.R.L.

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