Republic of the Philippines

Department of Education
REGION IV-A CALABARZON
SCHOOLS DIVISION OF LAGUNA PROVINCE
LAGUNA SENIOR HIGH SCHOOL
PROVINCIAL CAPITOL COMPOUND, POBLACION I SANTA CRUZ, LAGUNA

Frog Dissection Lab

Exploring Amphibian Anatomy

Objectives:
• Describe the appearance of various organs found in the frog.
• Name the organs that make up various systems of the frog.
Purpose:
In this lab, you will dissect a frog in order to observe the external and internal structures of frog anatomy.
Materials:
• gloves, face masks and a lab apron • dissecting tray
• dissecting kit • garbage bag
• 1 - large frog • Disinfecting alcohol
• dissecting pins (6–10) • Paper towels/ Tissue paper
Dissection Instructions
A. Place the frog in the dissecting pan ventral side up.
B. Use the scalpel to cut through the frog’s skin so you can
see the muscle tissues. Cut along the midline of the body
to the forelimbs.
C. Once you have looked at the muscle tissue, then use
your scissors or your scalpel to carefully cut through
muscle tissue without cutting the internal organs. Lift the
abdominal muscles away from the body cavity.
D. Cut along the midline of the body to the forelimbs.
Make transverse (horizontal) cuts near the arms and legs.
E. Locate each of the organs of the frog and take a photo,
remove each organ and place to the side of the dissecting
pan.
https://biologyjunction.com/frog-dissection/
*If your specimen is a female, the body may be filled with eggs. You may need to remove these eggs to
view the organs. Male frogs are also usually smaller than female frogs. Observe several frogs to see the
difference between males and females.

F. Locate each of the organs below. Use the diagram below to locate and identify the organs of the
digestive system: esophagus, stomach, small intestine, large intestine, cloaca, liver, gallbladder, and
pancreas.

G.
Use a probe and scissors to lift and remove the
intestines and liver. Use the diagram to identify
the parts of the urinary and reproductive systems.
Remove the peritoneal membrane, which is
connective tissue that lies on top of the red
kidneys. Observe the yellow fat bodies that are
attached to the kidneys. Find the ureters; the
urinary bladder; the testes and sperm ducts in
the male; and the ovaries, oviducts, and uteri in
the female.
H. Remove the kidneys and look for threadlike
spinal nerves that extend from the spinal cord.
Dissect a thigh, and trace one nerve into a leg muscle. Note the size and texture of the leg muscles.
I. Dispose of your materials according to the directions from your teacher.
J. Clean up your work area and wash your hands before leaving the lab.
 Frog Dissection Lab Activity

Complete the table: Take a photo of every organ that will be removed from the frog.
Name the organs and identify the function of each. Follow the example no. 1

Organs Function
1. Liver

2. Fat Bodies

3. Heart

4. Lungs

5. Gall Bladder

6. Stomach
7. Small Intestine
8. Large Intestine
9. Spleen
10. Esophagus

-
Label the diagram.

Post Lab Questions

1. The membrane holds the coils of the small intestine together: _________________________
2.This organ is found under the liver, it stores bile: ___________________________
3. Name the 3 lobes of the liver: _________________, ____________________, _______________
4. The organ that is the first major site of chemical digestion: _______________________
5. Eggs, sperm, urine and wastes all empty into this structure: __________________________
6. The small intestine leads to the: _______________________________
7. The esophagus leads to the: ______________________________
8. Yellowish structures that serve as an energy reserve: _________________________
9. The first part of the small intestine(straight part): ____________________________
10. After food passes through the stomach it enters the: _________________________
11. A web-like membrane that covers the organs: ___________________________
12. Regulates the exit of partially digested food from the stomach: _____________________
13. The large intestine leads to the _______________________
14. Organ found within the mesentery that stores blood: __________________________
15. The largest organ in the body cavity: __________________________