30/5/2024 15:47:17

Maria Fernanda Cervantes Sánchez (8117 bp)

CBD,T7 terminator,PstI,MluI,AgeI,StuI,BfuAI,+7 AmpR promoter,AmpR,BspHI,BssSI,BssSαI

BsaI,MreI,SgrAI ScaI
Mxe GyrA intein,ZraI,+5 PvuI
BsrGI,BsiWI FspI
EcoNI BsaI
MCS,MscI,EcoRI,+7 AhdI
SpeI 0
750
EcoRV SwaI
BamHI Amp PsiI
R
SwaI AloI
AleI,BtgI,+1 BsaAI
PciI DraIII
BseRI

M1
BglII BspHI

3
)

StuI
se

or
NA ge minida

i
NA CDS (ne 7164

ne
ura

MfeI
5725-

AlwNI

PsiI

ori
+1 Maria Fernanda Cervantes Sánchez
+1
BmtI
8117 bp +1
NheI
ATG PciI
MCS
+4

0
250
+3 BstZ17I
BsaAI,+2
+1 p
BsmBI
ro

Esp3I

+1
50
00
+1 EagI
lacI
rrnB T1 terminator AfeI
rrnB T1 terminator,+1 PshAI
SphI KasI,NarI,SfoI,PluTI,+2
lacI promoter,EcoNI EcoRV,HpaI
BclI,MluI BssHII
PspOMI,ApaI,BstEII

https://benchling.com/fercervantes/f/lib_mAqW4YAK-dna-sequence/seq_0DSAofYx-plasmidnaexamen-2darevision/edit 1/1