Abstract Book Issmart -Afob 2022

The 4th International Seminar on Smart Molecule of Natural Resources (ISSMART) – Asian
Federation of Biotechnology (AFOB) 2022 | i

OPENING SPEECH RECTOR OF UNIVERSITAS BRAWIJAYA
Assalamualaikum wr. wb.
On behalf of organizing committee of ISSMART 2022, I would like to extend my warmest

welcome to all delegates of ISSMART 2022 (4th International Seminar on Smart Molecules of
Natural Resources). Welcome to Malang, one of the educational cities in Indonesia. There are about
40 universities in Malang. The largest university in Malang is Universitas Brawijaya. Universitas
Brawijaya is also included in the top ten the best universities in Indonesia. This year, Universitas
Brawijaya is placed in the third rank, behind UI and UGM.
ISSMART 2022 has collaborated with AFOB (Asian Federation of Biotechnology), to have a
joint conference. This makes ISSMART 2022 is an exceptional event.
ISSMART is held annually, starting three years ago with ISSMART 2019. This conference is
organized by Research Centre of Smart Molecules of Natural Genetic Resources (SMONAGENES).
SMONAGENES is one of the Research Centre in Brawijaya University. SMONAGENES is also one
of the leading Research Centre in Brawijaya university. Also, one of top five Research Centre in
Brawijaya university.
ISSMART 2022 aimed to promote mutual exchange between scientists and experts, to discuss
of new research results in the fields of theoretical and experimental of smart molecules from natural
resources. The seminar facilitates of researchers and academic members or experts from universities,
government institutions, private sectors, and non-government organization to share their knowledge
through the discussion on plenary session, and parallel session of oral presentation.
I believe that ISSMART 2022 should bring advantages for all participants. They will learn
many new aspects of research in the related topics, either from keynote speakers or general
participants. In addition, collaborations between Universitas Brawijaya and other universities both
from Indonesia and overseas can be initiated.
Due to the corona virus outbreak or COVID-19 pandemic, ISSMART 2022 is going to be held
as a hybrid conference.
Finally, I would like to express my gratitude to keynote speakers, for their expertise and
knowledge they will bring to the conference, and of course the inevitable discussion of their talks.
Special thanks are also extended to the members of organizing committee for their hard work in
bringing this conference together. And last but not least, I would like to thank all of the conference
participants who will contribute to making this the most memorable ISSMART yet.
Federation of Biotechnology (AFOB) 2022 | ii
Please enjoy ISSMART 2022 and have a delightful seminar.
Wassalamulaikum wr. wb.
Sincerely yours
Rector of Universitas Brawijaya
Federation of Biotechnology (AFOB) 2022 | iii
WELCOME MESSAGE ISSMART 2022 CHAIRMAN
On behalf of the Organizing Committee, I would like to welcome all of you, keynote speakers,
invited speakers, distinguished guests, and participants, to the 4th International Seminar on Smart
Moelcule of Natural Resources – Asian Federation of Biotechnology (ISSMART – AFOB 2022).
The ISSMART – AFOB 2022 is an annual scientific meeting organized by Research Centre of
SMONAGENES (Smart Molecules of Natural Genetic Resource), Brawijaya University, Indonesia.
We are grateful that ISSMART 2022 has been joint conference with AFOB (Asian Federation of
Biotechnology) for second years.
This meeting aimed to promote mutual exchange between researchers and also experts, to
discuss innovative ideas in scientific research. The ISSMART – AFOB 2022 runs for 2 days, 24-25
August 2022 as a hybrid conference. However, I believe that the excitement to participate in
ISSMART – AFOB 2022 will not be lessened.
This year the is “Smart Molecules for Strengthening Community Life After Pandemic Era”, which
covers a broadrange of research field with various scopes of:
• Biochemistry
• Bioinformatics
• Biophysical Science
• Biomedical Engineering
• Herbal Medicine
• Nutrigenomics & Nutrigenetics
• Medical Physics
• Molecular Biology
• Neurosciences
This particular theme is intended to promote recent advances in the field of natural genetic
resources, importantly to address global issue that is outbreak of the corona virus. This international
forum also provides a platform where national and international academia or researchers, policy
makers, and other stakeholders to translate technology, exchange ideas, and help stimulate
multidisciplinary international collaborations in a convergent-manner for shaping a worldwide
sustainable development.
Federation of Biotechnology (AFOB) 2022 | iv
The selected papers of the conference will be published in Journal of Tropical Life Science or
in Journal Sains and Technology Indonesia. Both are indexed by Scopus. Also in Majalah Kesehatan
that nationally accredited as SINTA 2.
Around 70 people registered to this seminar. They consist of 43 presenting participants, about
11 non-presenting participants, and 6 keynote speakers.
I would also like to thank to our keynote speakers:
• Prof. Katsuhiro Mijayija from University of Agriculture, Japan.

• Assoc. Prof. Ts. ChM. Dr. Nik Ahmad Nizam Nik Malek from Universiti Teknologi, Malaysia.
• Prof. Fatchiyah, M.Kes., Ph.D from Brawijaya University.
• Prof. Dr. Mohamed Elsayed Mohamed from Zagazig University, Egypt.
• Dr. Nurhadi Ibrahim, Ph.D from The Department of Medical Physiology and Biophysics, Faculty
of Medicine, Universitas Indonesia.
• Prof. Montarop Yamabhai, Ph.D from School of Biotechnology, Suranaree University of
Technology, Thailand.
The ISSMART – AFOB 2022 could not become a reality without the help and assistance of
many parties. Thus, in this occasion I would like to sincerely thank to the Rector of Universitas
Brawijaya, Head of LPPM UB, Research Centre of SMONAGENES, and all of members of the
Organizing Committee, who have provided meaningful help and assistance for the implementation
of this conference. And last but not least, I would like to thank all of the conference participants who
will contribute to making this most memorable event of ISSMART – AFOB 2022.
Last but not least, we have tried to do our best to prepare ISSMART – AFOB 2022.
Nevertheless, there is nothing completely perfect in the world, including this conference. Therefore,
please accept our deep apologizes for any inconveniences found in this conference.
Please enjoy ISSMART – AFOB 2022.
Malang, 23 August 2022
Chairman of the Organizing Committee
Dr. dr. Nia Kurnianingsih, M.Biomed
Federation of Biotechnology (AFOB) 2022 | v
TABLE OF CONTENTS
OPENING SPEECH RECTOR OF UNIVERSITAS BRAWIJAYA ........................................ii
WELCOME MESSAGE ISSMART 2022 CHAIRMAN ..........................................................iv
TABLE OF CONTENTS ...........................................................................................................vi
SCIENTIFIC PROGRAM .........................................................................................................ix
Keynote Speaker
Application of urinary liver-type fatty acid-binding protein (L-FABP) on the mice DIC
(disseminated intravascular coagulation) models in COVID-19 study ………………………...1
Exploring Bio-Inspired Green Metal Nanoparticles Synthesis using Plant Extract ……………2
Overview Exploration of Natural Bioactive Compounds as Antioxidant, Anti-inflammatory, and
Antiaging for healthy cells nutrition ……………………………………………………………3
Infectious diseases transmitted between animals and humans with reference to Corona Virus .4
Molecular Docking Analysis of Centella asiatica’s compounds against Alzheimer's Diseases’
target protein due to Post-COVID-19 Infection ………………………………………………..5
Discovery of Specific Recombinant Antibody for Smart Theranostic …………………….....6
Invited Speaker
Regulatory mechanism of morphogen signals by the autism-related gene cdc2-like kinase 2
(clk2) in Xenopus neural development ......................................................................................8
Oral Presentation
Cell-based evidence to the Anti-COVID-19 potential of honeybee propolis ingredients ....…..9
The Effect of Serial Dilution on Viability 3T3-L1 Cell Line …………………………….…..10
Color Decoration of Chicken Egg Using Natural Dye Moleculein Sappan Wood ……..…….11
In Silico Study of Bioactive Compounds from Tangerines (Citrus reticulata) as Protein Inhibitor
for 3CLpro SARS-CoV-2 : Omicron Variant …………………………………………..…….12
The Effect of Black Cumin Extract in Self Nanoemulsifying Drug Delivery System (Snedds)
Preparation On Renal Histopathology After Diabetes Mellitus Induction With Alloxan …....13
The Potential of Ferulic Acid as an Anti-virulence to Combat Pathogenic Bacteria ……...…14
In Silico and ADMET Analysis of Potential Compounds in Licorice Root (Glycyrrhiza glabra)
as PPAR-γ Activator to Increase Insulin Sensitivity in Type 2 Diabetes Mellitus ……….......15
Comparison of The Effect Between Black Cumin (Nigella sativa) in Form of Self-
Nanoemulsifying Drug Delivery System (SNEDDS) and Non-SNEDDS on The Number of
Hippocampus Pyramid Cells of Alloxan-Induced Diabetic Rats (Rattus Norvegicus) ……….17
Computational Insight on Potential Activity of Star Apple (Chrysophyllum cainito L.) Bioactive
Compound as PPAR-γ Antagonist for Obesity ……………………………………………….18
Virtual Based Screening of Rosmarininc Acid and its Derivatives as Potential Anticancer
Approach Targeting HIF-1α …………………………………………………………………..19
The Potential of Bioactive Compounds from Cyperus rotundus as First Aid Medication on
Snakebite Envenomation by Bungarus candidus: In-silico Study ……………………………20
Difference Effect Between Black Cumin Seeds Extract (Nigella Sativa) in The Form of Self-
Nanoemulsifying Drug Delivery System (SNEDDS) and Non-SNEDDS on The Histopathology
of Hepar of Alloxan-Induced Diabetic Rattus Norvegicus: An Experimental Study …………21
In Silico Approach of Potential Compounds in Tomato (Solanum Lycopersicum) as
Antidiabetic ……………………………………………………………………………………22
Effect of Black Cumin Seed Extract in Self Nanoemulsifying Drug Delivery System (SNEDDS)
on Histopathological Islet Pancreas in Alloxan Diabetic Rattus norvegicus: An Experimental
Study …………………………………………………………………………………………..23
Federation of Biotechnology (AFOB) 2022 | vi
Effect of Black Cumin Extract in Self Nanoemulsifying Drug Delivery System Preparation on
Histopathological Appearance of Rattus Norvegicus Myocardium Post Alloxan Diabetes
Mellitus Induction ………………………………………………………………………….....24
Pathophysiological Analysis of kidney and comparison of male and female SDT fatty rats, an
obese model of type 2 diabetes mellitus, fed a high sucrose and high fat diet ………………..25
Investigation of the usefulness of hL-FABP tg mice as biomarkers for NAFLD/NASH. …....26
Anti-angiogenic effect of non-contact electro capacitive cancer therapy on DMBA-induced rat
breast tumor model …………………………………………………………………………....27
Enzymatic Synthesis and Cytotoxicity Activity of Phenolic Acid Glucosyl Ester in
Cholangiocarcinoma Cells ……………………………………………………………………29
BRAZILIN AND 3 – O- METHYL BRAZILIN REVEALED THERAPEUTIC AGENT FOR
ECZEMA: MOLECULAR DOCKING APPROACH ………………………………………..30
Anti-inflammatory effects of Channa micropeltes extract through NF-κβ and TNF-α in diabetic
rat ………………………………………………………………………………………….......31
Computational and in vitro experimental evidence to the anti-migration activity of Cucurbitacin-
B ……………………………………………………………………………………………....32
In vitro cell culture-based evaluation of antistress potentials of fucoxanthin ………………...33
Phytochemical Screening and In-vitro Antioxidant Activity of leaf extract of Clinacanthus
Nutans …………………………………………………………………………………………………....34
Lactate dehydrogenase (LDH) in the vagina of ratus norvegicus wistar after exposure to candida
albicans at 12, 24 and 48 hours …………………………………………………………….....35
Pharmaceutical Application of Microcapsules from The Combination of Ruellia tuberosa L. and
Cosmos caudatus K. Extracts ………………………………………………………………....36
Effect of Recombinant Protein Nano-vaccine C. vulgaris on Interferon-Gamma Gene Response
and Growth in Grouper ………………………………………………………………………..37
IOT-Based Microbubble Aerator Application for Water Quality Control in Catfish Farming at
Mina Berkah Sejahtera Farmers Community, Situbondo …………………………………….38
MortaparibMild- A novel small molecule inhibitor of mortalin inhibits proliferation and migration
of cancer cells by multiple pathways …………………………………………………………39
Alginate-Based Microencapsulation of Ruellia tuberosa L. Ethanolic Extract and Their Activities
as Alpha-Amylase Inhibitor …………………………………………………………………..40
Effect of acetic acid in inhibition Pseudomonas aeruginosa biofilm in chronic suppurative otitis
media (in vitro study) …………………………………………………………………………42
Medication Adherence Outpatients Type 2 Diabetic Mellitus at Four Primary Health Care Centers
in Medan City, Indonesia ……………………………………………………………………..43
Anthocyanin From Purple Sweet Potatoes Modify Visceral Fat Tissue Inflammation and Hepatic
Stress Oxidative in Chronic Restraint Stress-Induced Mice ………………………………….44
Correlation Between p53 and Ki67 with Aggressiveness Factor in Recurrent Respiratory
Papillomatosis ………………………………………………………………………………...45
Metabolite Profiles and Hypoglycemic Activities of Seed Extract of Pinang (Areca catechu
L.) ……………………………………………………………………………………………..46
Correlation between Serum Levels of Matrix Metalloproteinases 1 and 9 with Cavity and Fibrosis
in Multidrug-Resistant Subjects Using High-Resolution Computerized Tomography of the
Thorax ………………………………………………………………………………………...47
SLEEP DISTURBANCES SCREENING AMONG ADOLESCENTS IN BATU, EAST JAVA,
INDONESIA ………………………………………………………………………………….48
Microencapsulation of Ethanolic Leaves Extract of Cosmos caudatus Kunth Using Gum Arabic
in various pH and Extract concentration. …………………………………………………….49
Development of Smart Deploy Web Big Data App for Determining Modeling of Medicinal
Compounds to Healing Various Disease Especially for Covid-19 and Its New Variants Using
Quantum Meta-Deep AI Algorithm …………………………………………………………..51
Federation of Biotechnology (AFOB) 2022 | vii
Detection of Myxobolus sp. infection (Myxosporeasis) on Cultured Koi fish in Blitar District,
East Java Province …………………………………………………………………………….53
The Molecular Prediction of Indonesian Medicinal Plants' Activity on 5-HT2a Receptor Involved
in Schizophrenia ………………………………………………………………………………54
The Addition of Red Dragon Fruit Peel and Lemon Peel in The Development of Coconut Water
Fermented Beverage Products using Lactic Acid Bacteria to Decrease Tannin Levels and Increase
Antioxidant Activity …………………………………………………………………………..55
Design of multifunctional engineered-modular endolysin as a ‘tailor-made’ antimicrobial
candidate for Streptococcal and Staphylococcal infections …………………………………..57
Effect of Water Clover (Marsilea crenata Presl.) extract on SOD1 and Nrf2 Expression Level of
Male Rat (Rattus norvegicus) Testis .........................................................................................58
.....
Federation of Biotechnology (AFOB) 2022 | viii
SCIENTIFIC PROGRAM
THE 4th
INTERNATIONAL SEMINAR ON SMART MOLECULE OF NATURAL
RESOURCES (ISSMART) – ASIAN FEDERATION OF BIOTECNOLOGY (AFOB)
2022
Link Zoom Meeting for Plenary Session (Main Room)

https://us02web.zoom.us/j/83032962460?pwd=RTk3WU9SWlMzL0ZZeVk0WWE3TVpSZz09
Meeting ID : 830 3296 2460
Passcode : 4thissmart
PLENARY SESSION
Wednesday, 24th August 2022 (DAY 1)
07.30– 08.30 WIB : Registration
08.30 – 08.35 WIB : Welcome speech from ISSMART-AFOB 2022 Chair
08.35 – 08.45 WIB : Opening speech from Rector Universitas Brawijaya
Moderator: Tomohiko Sasase, Ph.D

08.45 – 09.15 WIB : Keynote speaker speech
10.45 - 11.15 (Japan Time)
Prof. Katsuhiro Miyajima (Tokyo University of Agriculture, Japan)
"Application of urinary liver-type fatty acid-binding protein (L-FABP) on the mice DIC
(disseminated intravascular coagulation) models in COVID-19 study"

dr. Nurhadi Ibrahim, PhD. (The Department of Medical Physiology and Biophysics, Faculty of
Medicine, Universitas Indonesia)
"Molecular Docking Analysis of Centella asiatica’s compounds against Alzheimer's Diseases’ target
protein due to Post-COVID-19 Infection”

04.45- 05.15 (Egypt Time)
Prof. Dr. Mohamed Elsayed Mohamed (Zagazig University, Egypt)
"Infectious diseases transmitted between animals and humans with reference to Corona Virus"
10.15 – 10.30 WIB : Discussion

10.30 – 10.35 WIB : Awarding of certificates for keynote speaker
10.35 – 10.55 WIB : Presentation from Prof. Renu Wadhwa, Ph.D and Discussion
12.35- 12.55 (Japan Time)
"Cell-based evidence to the Anti-COVID-19 potential of honeybee propolis ingredients"
10.55 – 12.00 WIB : Lunch Break
12.00 – 14.00 WIB : Oral presentation for offline participant (Hybrid)
Federation of Biotechnology (AFOB) 2022 | ix
PLENARY SESSION
Thursday, 25th August 2022 (DAY 2)
07.30 – 08.00 WIB : Registration
Moderator : Regina Putri Virgirinia, Ph.D

Prof. Fatchiyah, M.Kes., PhD. (Universitas Brawijaya, Indonesia)
"Overview Exploration of Natural Bioactive Compounds as Antioxidant, Anti-inflammatory, and
Antiaging for healthy cells nutrition "

09.30- 10.00 (Malaysia Time)
Assoc. Prof. Ts. ChM. Dr. Nik Ahmad Nizam Nik Malek (Universiti Teknologi Malaysia)
"Exploring Bio-Inspired Green Metal Nanoparticles Synthesis using Plant Extract"

09.00- 09.30 (Thailand Time)
Prof. Montarop Yamabhai, PhD. (School of Biotechnology, Institute of Agricultural Technology,
Suranaree University of Technology, Thailand)
“Discovery of Specific Recombinant Antibody for Smart Theranostics”
09.30 – 09.45 WIB : Discussion

09.45 – 09.50 WIB : Awarding of certificates for keynote speaker
09.50 – 10.00 WIB : ResearchHub as a solution for accelerating biomedical research in Indonesia
10.00 - 10.05 : Break
10.00 – 12.00 WIB : Oral Presentation for online participant (Hybrid)
12.00 – 12.10 WIB : Closing ceremony and awards
Federation of Biotechnology (AFOB) 2022 | x
ORAL PRESENTATION SCHEDULE-DAY 1 (Wednesday, 24th August 2022)
Room : MC 1.1
Link Zoom Meeting for Plenary Session (Main Room)
https://us02web.zoom.us/j/83032962460?pwd=RTk3WU9SWlMzL0ZZeVk0WWE3TVpSZz09
Meeting ID : 830 3296 2460
Passcode: 4thissmart
Moderator : Yeni Avidhatul Husnah, M.Si
Times Presenter Title
12.00 - 12.10 Regina Putri Virgirinia (Amphibian Regulatory mechanism of morphogen
Research Center, Graduate School signals by the autism-related gene cdc2-like
of Science, Hiroshima University, kinase 2 (clk2) in Xenopus neural
Japan; Research Center of Smart development
Molecule of Natural Genetics
Resource, Brawijaya University,
Malang, East Java)
12.10 - 12.20 Tutik Setianingsih (Department of Color Decoration of Chicken Egg Using
Chemistry, Brawijaya University) Natural Dye Molecule in Sappan Wood
12.20 - 12.30 Cicin Vinolia Alvionita (Faculty of The Effect of Serial Dilution on Viability
Mathematics and Natural Sciences, 3T3-L1 Cell Line
Brawijaya University)
12.30 - 12.40 Daniel Tumbur Hamonangan Sirait IN SILICO STUDY OF BIOACTIVE

(Faculty of Mathematics and COMPOUNDS FROM TANGERINES
Natural Sciences, Brawijaya (CITRUS RETICULATA) AS PROTEIN
University) INHIBITOR FOR 3CLPRO SARS-COV-2 :
OMICRON VARIANT
12.40 - 12.50 Dinda Nawang Sari (Faculty of The Effect of Black Cumin Extract in Self
Medicine, Universitas Islam Nanoemulsifying Drug Delivery System
Indonesia) (Snedds) Preparation On Renal
Histopathology After Diabetes Mellitus
Induction With Alloxan
12.50 - 13.00 Discussion
13.00 - 13.10 Ernanin Dyah Wijayanti (Faculty The Potential of Ferulic Acid as an Anti-
of Mathematics and Natural virulence to Combat Pathogenic Bacteria
Sciences, Brawijaya University)
13.10 - 13.20 Maurin Ardhina Syafiudin (Faculty In Silico and ADMET Analysis of Potential
of Mathematics and Natural Compounds in Licorice Root (Glycyrrhiza
Sciences, Brawijaya University) glabra) as PPAR-γ Activator to Increase
Insulin Sensitivity in Type 2 Diabetes
Mellitus
Federation of Biotechnology (AFOB) 2022 | xi
13.20 - 13.30 Muhammad Zenryu Asmara Comparison of The Effect Between Black
(Faculty of Medicine, Universitas Cumin (Nigella sativa) in Form of Self-
Islam Indonesia) Nanoemulsifying Drug Delivery System
(SNEDDS) and Non-SNEDDS on The
Number of Hippocampus Pyramid Cells of
Alloxan-Induced Diabetic Rats (Rattus
Norvegicus)
Federation of Biotechnology (AFOB) 2022 | xii
Room : MC 1.2 / Breakout Room B
Moderator : Ernanin Dyah Wijayanti, M.P
12.00 - 12.10 Nabila Nur Rosyada (Faculty of Computational Insight on Potential
Mathematics and Natural Sciences, Activity of Star Apple (Chrysophyllum
Brawijaya University) cainito L.) Bioactive Compound as
PPAR-γ Antagonist for Obesit
12.10 - 12.20 Nisa Nabila Aufa Wachid (Faculty Virtual Based Screening of Rosmarininc
of Mathematics and Natural Acid and its Derivatives as Potential
Sciences, Brawijaya University) Anticancer Approach Targeting HIF-1α
12.20 - 12.30 Rahmat Grahadi (Faculty of The Potential of Bioactive Compounds
Mathematics and Natural Sciences, from Cyperus rotundus as First Aid
Brawijaya University) Medication on Snakebite Envenomation
by Bungarus candidus: In-silico Study
12.30 - 12.40 Rulianty Febriani (Universitas Difference Effect Between Black Cumin
Islam Indonesia) Seeds Extract (Nigella Sativa) in The
Form of Self-Nanoemulsifying Drug
Delivery System (SNEDDS) and Non-
SNEDDS on The Histopathology of
Hepar of Alloxan-Induced Diabetic
Rattus Norvegicus: An Experimental
Study
12.50 - 13.00 Sekararum Narwasthu (Faculty of In Silico Approach of Potential
Mathematics and Natural Sciences, Compounds in Tomato (Solanum
Brawijaya University) Lycopersicum) as Antidiabetic
13.00 - 13.10 Syafira Laila Nurulita (Universitas Effect of Black Cumin Seed Extract in
Islam Indonesia) Self Nanoemulsifying Drug Delivery
System (SNEDDS) on Histopathological
Islet Pancreas in Alloxan Diabetic Rattus
norvegicus: An Experimental Study
13.10 - 13.20 Talenta Nugroho Suryanto Effect of Black Cumin Extract in Self
Mahardhika (Faculty of Medicine, Nanoemulsifying Drug Delivery System
Universitas Islam Indonesia) Preparation on Histopathological
Appearance of Rattus Norvegicus
Myocardium Post Alloxan Diabetes
Mellitus Induction
Federation of Biotechnology (AFOB) 2022 | xiii
ORAL PRESENTATION SCHEDULE-DAY 2 (Thursday, 25th August 2022)
Breakout Room A
Moderator : Tomohiko Sasase, Ph.D
10.00 - 10.10 Kana Watanabe (Tokyo University Pathophysiological Analysis of kidney and
of Agriculture, Japan) comparison of male and female SDT fatty
rats, an obese model of type 2 diabetes
mellitus, fed a high sucrose and high fat
diet
10.10 - 10.20 Marika TOHMA (Tokyo University Investigation of the usefulness of hL-
of Agriculture, Japan) FABP tg mice as biomarkers for
NAFLD/NASH.
10.20 - 10.30 Endah Sri Palupi (Faculty of Anti-angiogenic effect of non-contact
Biology, Universitas Gadjah Mada) electro capacitive cancer therapy on
DMBA-induced rat breast tumor model
10.40 - 10.50 Eko Suyanto (Brawijaya University) Enzymatic Synthesis and Cytotoxicity
Activity of Phenolic Acid Glucosyl Ester
in Cholangiocarcinoma Cells
10.50 - 11.00 Dewi Ratih Tirto Sari (Faculty of BRAZILIN AND 3 – O- METHYL
Medical Science, Universitas BRAZILIN REVEALED THERAPEUTIC
Ibrahimy) AGENT FOR ECZEMA: MOLECULAR
DOCKING APPROACH
11.00 - 11.10 Juliyatin Putri Utami (University of Anti-inflammatory effects of Channa
Lambung Mangkurat, Indonesia) micropeltes extract through NF-κβ and
TNF-α in diabetic rat
11.10 - 11.20 Hariyatun Hariyatun (Research Design of multifunctional engineered-
Center for Genetic Engineering, modular endolysin as a ‘tailor-made’
National Research and Innovation antimicrobial candidate for Streptococcal
Agency) and Staphylococcal infections
11.20 - 11.30 Sri Rahayu (Biology Department, Effect of Water Clover (Marsilea crenata
Brawijaya University) Presl.) extract on SOD1 and Nrf2
Expression Level of Male Rat (Rattus
norvegicus) Testis
Breakout Room B
Moderator : Ernanin Dyah Wijayanti, M.P
10.00 - 10.10 He Huifu (University of Tsukuba, Computational and in vitro experimental
Japan) evidence to the anti-migration activity of
Cucurbitacin-B
Federation of Biotechnology (AFOB) 2022 | xiv
10.10 - 10.20 Huayue Zhang (University of In vitro cell culture-based evaluation of
Tsukuba, Japan) antistress potentials of fucoxanthin
10.20 - 10.30 Nurlaili Susanti (Faculty of Phytochemical Screening and In-vitro
Medicine, Universitas Airlangga) Antioxidant Activity of leaf extract of
Clinacanthus nutans
10.20 - 10.40 Nurdiana (Faculty of Medicine, Lactate dehydrogenase (LDH) in the vagina
Universitas Brawijaya) of ratus norvegicus wistar after exposure to
candida albicans at 12, 24 and 48 hours
10.50 - 11.00 Choirin Annisa (Faculty of Pharmaceutical Application of
Mathematis and Natural Sciences, Microcapsules from The Combination of
Brawijaya University) Ruellia tuberosa L. and Cosmos caudatus K.
Extracts
11.00 - 11.10 Uun Yanuhar (Faculty of Fisheries IOT-Based Microbubble Aerator
and Marine Sciences, University of Application for Water Quality Control in
Brawijaya) Catfish Farming at Mina Berkah Sejahtera
Farmers Community, Situbondo
11.10 - 11.20 Uun Yanuhar (Faculty of Fisheries Cell-based evidence to the Anti-COVID-19
and Marine Sciences, University of potential of honeybee propolis ingredients
Brawijaya)
Breakout Room C
Moderator : Ema Pristi Yunita, S. Farm., M. Farm., Klin., Apt.
10.00 - 10.10 Shi Yang (University of Tsukuba, MortaparibMild- A novel small molecule
Japan) inhibitor of mortalin inhibits proliferation
and migration of cancer cells by multiple
pathways
10.10 - 10.20 Andriana Kusuma Pertiwi (Faculty Alginate-Based Microencapsulation of
of Mathematis and Natural Ruellia tuberosa L. Ethanolic Extract and
Sciences, Brawijaya University) Their Activities as Alpha-Amylase
Inhibitor
10.20 - 10.30 Artono (Faculty of Medicine, Effect of acetic acid in inhibition
Universitas Airlangga) Pseudomonas aeruginosa biofilm in
chronic suppurative otitis media (in vitro
study)
10.20 - 10.40 Embun Suci Nasution (Faculty of Medication Adherence Outpatients Type 2
Pharmacy, Universitas Sumatera Diabetic Mellitus at Four Primary Health
Utara) Care Centers in Medan City, Indonesia
Federation of Biotechnology (AFOB) 2022 | xv
10.50 - 11.00 Nia Kurnianingsih (Faculty of Anthocyanin From Purple Sweet Potatoes
Medicine, Universitas Brawijaya) Modify Visceral Fat Tissue Inflammation
and Hepatic Stress Oxidative in Chronic
Restraint Stress-Induced Mice
11.00 - 11.10 Rizka Fathoni Perdana (Faculty of Correlation Between p53 and Ki67 with
Medicine, Universitas Airlangga) Aggressiveness Factor in Recurrent
Respiratory Papillomatosis
11.10 - 11.20 Trini Suryowati (Medical College, Metabolite Profiles and Hypoglycemic
Universitas Kristen Indonesia) Activities of Seed Extract of Pinang (Areca
catechu L.)
Breakout Room D
Moderator : Yeni Avidhatul Husnah, M.Si
10.00 - 10.10 Anse Diana Valentiene Messah Corelation serum levels MMP-1 and
(Biomedical Science Faculty Of MMP-9 with cavity and fibrosia at
Medicine, University of Indonesia) Multidrug resistance isinya Cheta HRCT
10.10 - 10.20 Novita Titis Harbiyanti (Faculty of SLEEP DISTURBANCES SCREENING

Medicine, Universitas Brawijaya) AMONG ADOLESCENTS IN BATU, EAST
JAVA, INDONESIA
10.20 - 10.30 Anna Roosdiana (Faculty of Microencapsulation of Ethanolic Leaves Extract
Mathematis and Natural Sciences, of Cosmos caudatus Kunth Using Gum Arabic
Brawijaya University) in various pH and Extract concentration
10.20 - 10.40 Imam Cholissodin (Faculty of Development of Smart Deploy Web Big Data
Computer Science, Brawijaya App for Determining Modeling of Medicinal
University) Compounds to Healing Various Disease
Especially for Covid-19 and Its New Variants
Using Quantum Meta-Deep AI Algorithm
10.50 - 11.00 Muchammad Yunus (Faculty of Detection of Myxobolus sp. infection
Veterinary Medicine, Airlangga (Myxosporeasis) on Cultured Koi fish in Blitar
University) District, East Java Province
11.00 - 11.10 Wike Astrid Cahayani (Faculty of The Molecular Prediction of Indonesian
Medicine, Universitas Brawijaya) Medicinal Plants' Activity on 5-HT2a Receptor
Involved in Schizophrenia
11.10 - 11.20 Sofi Nabila (Faculty of The Addition of Red Dragon Fruit Peel and
Mathematics and Natural Sciences, Lemon Peel in The Development of Coconut
Brawijaya University) Water Fermented Beverage Products using
Lactic Acid Bacteria to Decrease Tannin Levels
and Increase Antioxidant Activity
Federation of Biotechnology (AFOB) 2022 | xvi
Federation of Biotechnology (AFOB) 2022 | xvii
Keynote Speaker
Application of urinary liver-type fatty acid-binding protein

(L-FABP) on the mice DIC (disseminated intravascular
coagulation) models in COVID-19 study
Prof. Katsuhiro Miyajima
Tokyo University of Agriculture, Japan
Severe acute respiratory syndrome coronavirus has spread all over the world, causing a pandemic. Appropriate
intervention for coronavirus disease 2019 (COVID-19) patient at early stage is required for improvement of
prognosis and subsequent complications. The number of severe cases of COVID-19 has increased and the
medical system has reached near its limits, therefore, there is a need to develop effective biomarkers to
diagnose patients at high risk of progressing to severe disease at an early stage. Liver-type fatty acid binding
protein (L-FABP) has potential utility as early prognostic biomarker ahead of traditional severity scores. We
investigated the background mechanisms of urinary L-FABP excretion through non-clinical animal models of
thrombus formation induced by histone or LPS injection, which is a cause of the aggravating factors in
COVID-19. It has been reported that the risk of severe symptoms of COVID-19 is enhanced in the patients
with obese and diabetes mellitus. Therefore, we also examined the responsiveness of L-FABP to the induction
of underlying diseases such as lifestyle-related diseases.Taking it into consideration, 12- or 13-week-old male
human L-FABP chromosomal transgenic mice (hLFABP tg-mice) were used to reproduce COVID-19-
associated thrombosis, then examined the relation between urinary concentration of the human L-FABP and
histopathological findings in kidney. To evaluate the effects of underlying disease, hLFABP tg mice were
given streptozotocin (STZ) injections, a high-fat diet (HFD), or both STZ and HFD, each of these groups was
defined as diabetic, obese, and metabolic syndrome groups, respectively. In addition, to determine the efficacy
of therapeutic agents, histones were combined with anticoagulants and anti-inflammatory agents, and the
usefulness of urinary L-FABP as a biomarker was also investigated.The results revealed that concentration of
urinary L-FABP significantly correlated with histopathological findings in both LPS and histone
administration tests. LPS and histone administration caused the increase in urinary L-FABP at the time of
observation. In the groups with underlying disease, the concentration of urinary L-FABP further increased
after being administered with histone, compared to the control group. This concentration then decreased in the
drug-combined administered groups. In histopathology, LPS and histone administration caused inflammatory
cell infiltration and urinary casts in kidney. Urinary casts are also observed in the groups with underlying
disease and the drug-combined administered groups in different levels, which showed the impact of each factor
on DIC models. Overall, we suggest that urinary L-FABP could be used as a potential biomarker reflecting
hemodynamic change. This research is hoped to contribute to develop treatments and prevention methods for
COVID-19.
Federation of Biotechnology (AFOB) 2022 | 1
Keynote Speaker
Exploring Bio-Inspired Green Metal Nanoparticles Synthesis

using Plant Extract
Assoc. Prof. Ts. ChM. Dr. Nik Ahmad Nizam Nik Malek
Universiti Teknologi, Malaysia
Nanomaterials such as silver and gold nanoparticles are widely used in various applications. Because of the
tiny particles of these metals, it is a wonderful material with distinct properties. The properties of materials
at nanoscale differ from those at bulk scale. Because nanomaterials have a larger surface area, they are more
reactive and can be used in a variety of applications, such as antimicrobial agents. Nanomaterials can be
synthesized in the lab with the help of a chemical reducing agent, but the hazardous chemicals cause problems
in the final product. Aside from that, the physical method of nanoparticle synthesis requires a lot of energy
and radiation. As a result, the more sustainable biological synthesis approach is preferred. Fruit extract,
bacteria, honey, fungi, microalgae, and plant extract can all be used to reduce Ag+ to Ag0 (silver
nanoparticles). Plant extract is more desirable than microorganisms because it is easier to prepare and more
readily available due to its abundance. The plant extract contains a variety of bioactive compounds that can
be used as an alternative to chemical reducing agents. In this study, we investigated some important
Malaysian herbs as bioreducing agents for the synthesis of silver and gold nanoparticles. According to our
preliminary findings, there is a strong relationship between antioxidant and reducing ability and metal
nanoparticle synthesis yield. Because of the bioactive compounds responsible for reducing Ag+ to Ag0, plant
extracts with high antioxidant activity yield a high amount of silver nanoparticles (AgNP). The studied
Malaysian herbs are Persicaria odorata (or Polygonum minus) (Kesum), Diplazium esculentum (Pucuk
paku), Strobilanthes crispa (Pecah kaca), Orthosiphon aristatus (Misai kucing), Clinacanthus nutans
(Belalai gajah) and Ficus deltoidea (Mas cotek). These herbs can be used to produce silver nanoparticles, but
their synthesis parameters and yield differ. This is due to the fact that the bioactive compounds found in each
plant differ. The majority of them, however, contain bioactive compounds with high antioxidant activity.
They all produced silver nanoparticles with sizes ranging from 20 to 5 nm and spherical particle shapes. In
our study, we used silver nanoparticles as antimicrobial agents against various types of bacteria and wound
healing agents. Results from in vitro antibacterial and cytotoxicity assays and in vivo animal testing studies
showed promising results in which the biosynthesized silver nanoparticles are safe for humans and have high
antibacterial activity against a wide spectrum of bacteria. The benefits of using plant extract as bioreducing
agent on an industrial scale are avoiding chemical waste, hence reducing the production cost. We are planning
to commercialize the biosynthesized silver nanoparticles in the future, but the large-scale production needs
to be initially optimized. Other local herbs can be used as bioreducing agent; however, the exploration of the
plant analysis and fundamental synthesis parameters need to be initially studied.
Keynote Speaker
Overview Exploration of Natural Bioactive Compounds as

Antioxidant, Anti-inflammatory, and Antiaging for healthy
cells nutrition
Fatchiyah, Fatchiyah1,2
1
Dept. Biology, Faculty of Mathematic & Natural Science,
Brawijaya University, Jl Veteran, Malang 65145, INDONESIA
2
RC Smart Molecule of Natural Genetic Resources UB
*Corresponding author: fatchiya@ub.ac.id
Healthy cells provide structure for the body, take nutrients from food, convert them into energy, and perform
special functions. Eating a healthy diet can help maintain healthy cells, promote healthy cell aging and reduce
the risk of developing chronic diseases and controling metabolic disorders. Cells require proper nutrition for
metabolic regulation processes at the cellular level. The cellular nutrition helps remove active toxins in cells
and is able to absorb all the essential nutrients needed to function properly. Indonesia has various varieties of
flora and fauna containing bioactive compounds and secondary metabolites that have high nutritional value
and can be recommended as functional foods. The content of bioactive compounds or secondary metabolites
derived from local varieties has activity as an antioxidant, antiobesity, anti-inflammatory, antiaging, or other
functions that have healthy benefits for controlling cellular mechanisms. The energy consumption limits
longevity, age-related changes are associated with energy availability mainly from the mitochondria and
declining mitochondria function. These changes are caused by a decrease in the volume, integrity, and function
of mitochondrial DNA due to accumulation of mutations and oxidative damage caused by reactive oxygen
species (ROS). In addition, mammalian sirtuin (SIRT1-7) controls nutrients associated with physiological
responses to diet and exercise, and high expression of SIRT1 in tissues after calorie restriction (CR) may be
an influencing factor life-prolonging in diverse species. In this study will focus on the exploration of natural
bioactive compounds as antioxidants, anti-inflammatory, and antiaging for healthy cell nutrition. Indonesian
pigmented rice consists of many varieties, such as brown, red, purple, and black. Due to the rich nutritional
landscape and antioxidant nutraceuticals, pigmented rice varieties have the potential to improve human health
by managing various metabolic disorders. The diversity of Indonesian local pigmented rice and its potential to
reduce the risk of hyperlipidemia, and hyperglycemia, as well as for the prevention of cancer or metabolic
diseases. Recently our study demonstrated that a mouse model of dyslipidemia fed a diet of black rice extract
succeeded in reducing the susceptibility of the molecular pathway to dyslipidemia. In addition, Indonesian red
rice anthocyanins exhibit anti-oxidant and anti-diabetic functions that prevent the AGEs-RAGE interactions,
and Indonesian purple rice ferulic acid is potentially indicated as antimicrobial, anti-inflammatory, and anti-
aging properties.
Keynote Speaker
Infectious diseases transmitted between animals and humans

with reference to Corona Virus
Prof. Dr. Mohamed Elsayed Mohamed
Department of Zoonotic Diseases, Faculty of Veterinary Medicine, Zagazig University, Egypt.
bishet68@yahoo.de
Zoonoses (Z) may be defined as diseases or infections that are naturally transmitted from vertebrate animals
to humans. It was reported that 1415 species of pathogens known to infect humans, 868(61%) were zoonotic.
They are classified according to their reservoir hosts (Anthropozoonoses, Zooanthroponoses,
Amphixinoses). According to maintenance cycle in nature, they are direct zoonoses, Cyclozoonoses,
Metazoonoses and Saprozoonoses. Regarding the causative agents, they are Bacterial, Mycotic, Viral,
Rickettsial, Chlamydial and Parasitic Z. Concerning localities; they are rural and urban Z. The special types
are emerging Z and Occupational Z.
Coronaviruses are family of RNA viruses in the Nidovirales order, significant viral pathogens in human and
animal. Corona Prefix comes from the Latin word for crown. They are classified into 4 types: Alpha, Beta,
Gamma and Delta. Human Coronaviruses are Alpha and Beta Coronaviruses. They are medium sized viruses
enveloping a positive-stranded RNA. Coronaviruses infect birds and mammals. Bats are hosts to the largest
number of viral genotypes of them. Epidemics can occur when viruses transmit from one species to another.
They can cause mutations in proteins that allow viruses to bind to cells. Human Coronaviruses are causes of
respiratory and gastrointestinal tract infections. There are seven strains of human CoVs, which include NL63,
229E, HKU1, OC43, Middle East respiratory syndrome (MERS-CoV), severe acute respiratory syndrome
(SARS-CoV or SARS-CoV1), and 2019-novel coronavirus (SARS-CoV2)
The history of coronaviruses: Severe Acute respiratory Syndrome (SARS)-2002/2003 which are transmitted
from bats to civets to humans, began in Feb (2003 China) and outbreak lasted until July 2003 (774 death,
fatality rate 9.6%). Middle East Respiratory Syndrome (MERS) started in Saudi Arabia (2012), transmitted
from bat to camel to humans (858 deaths, fatality rate 34.4%). COVID-19 started in China, isolated from
snakes, pangolin and bats in animal markets.
As of 27 February 2022, over 433 million confirmed cases and over 5.9 million deaths have been reported
globally. At the regional level, the Western Pacific Region reported a 32% increase in the number of new
weekly cases while all other regions reported decreases. The number of new weekly deaths increased in the
Western Pacific (+22%) and the Eastern Mediterranean (+4%) Regions, whilst a decreasing trend have been
reported by the Regions of Africa (-59%), South-East Asia (-18%), Europe (-13%) and Americas (-8%).
Key words: Zoonoses, Corona, virus, public health.
Keynote Speaker
Molecular Docking Analysis of Centella asiatica’s compounds

against Alzheimer's Diseases’ target protein due to Post-
COVID-19 Infection
Dr. Nurhadi Ibrahim
Department of Medical Physiology and Biophysics, Faculty of Medicine, Universitas Indonesia
Background: A recent clinical study found that SARS-CoV-2 infection had a 3.5 times increased risk of being
diagnosed with Alzheimer’s disease (AD) and SARS-CoV-2-infected patients with AD had a higher rate of
death due to the disease than SARS-CoV-2-infected patients without AD. As the pandemic persists, we face
post-covid effects: a substantial number of individuals may develop short-, medium- and long-term
neurodegenerative diseases which have a major impact on everyday activities and quality of life. However, the
medications currently available to treat AD have limited efficacy and are associated with side effects. Natural
products are one of the most vital and conservative sources of medicines for treating neurological problems.
Centella asiatica (L.) Urb. (CA) is an important medicinal plant with a wide range of ethnomedicinal uses. To
evaluate the neuroprotective effect of CA compounds, in silico studies using molecular docking against three
putative targets for AD were conducted.
Methods: We used seven compounds of Indonesian CA, i.e asiatic acid, asiaticoside, madecassic acid,
madecassoside, madasiatic acid, and terminolic acid. For the protein target, we used ACE (angiotensin-
converting enzyme; PDB ID: 1O86), BACE1 (b-site APP cleaving enzyme 1; PDB ID: 4DJU), and GSK-3
(glycogen synthase kinase-3; PDB ID: 1Q5K). Molecular docking was conducted using Autodock Vina, in
UNIX-based scripts.
Results: The docking scores (kcal/mol) showed high potency against AD through inhibition of BACE1 and
GSK-3 mechanisms. Asiaticoside showed as the best inhibitor to BACE1 and GSK-3, with docking scores of
-10.50 kcal/mol and -8.84 kcal/mol, respectively. All the tested compounds didn’t have good inhibitory
activities on ACE protein.
Conclusions: Overall, the potential binding affinity from molecular docking suggests that CA could be
considered a suitable therapeutic lead for AD treatment post- COVID-19 infection by inhibiting BACE1 and
GSK-3 receptors.
Keywords: Alzheimer's disease, Centella asiatica, molecular docking, post-COVID-19
Keynote Speaker
Discovery of Specific Recombinant Antibody for Smart

Theranostics
Prof. Montarop Yamabhai, Ph.D
School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology
Theranostics is a novel concept of smart medicinal strategy that combine the ability to identify target of disease
for high precision delivery of desired therapeutic agent. The term "theranostic" is a combination of the word
therapeutics and diagnostics, implying the integration of diagnosis and therapy. While the current application
of theransotics is mainly in the field of nuclear medicine for targeted-based treatment of cancer; this emerging
field is expanding to include the use of various types of nanoparticle for personalized/precision medicine. The
main component of theranostics approach for disease treatment is the highly specific affinity moiety that can
be incorporated and direct the diagnostic/therapeutic reagents to the target of interest. One of the most effective
approaches to identify an affinity fragment for incorporation into a variety of theragnostic platform is by
affinity selection of peptide or antibody fragment using phage display technology, of which the co-inventors,
George P. Smith and Sir Gregory P. Winter, were award the Nobel Prize in Chemistry in 2018. This technology
has been employed in our laboratory for the discovery of affinity reagents against various targets, including
variable regions of monoclonal antibodies (mAb) 1-5, chitin 6 mycotoxins, viral particles 7, cancer and bacterial
cell surfaces 8,9. Our phage display peptide library (SUT-12), comprising random short linear peptide (12-
amino acid long) 10 has been used to generate specific epitopes and mimotopes to almost all mAb tested as
well as chitin polymer 11. In 2008, a compact phage display human single chain variable fragment (scFv)
antibodies library was constructed in our laboratory from peripheral blood mononuclear cells (PBMC) of 140
healthy volunteers in Nakhon Ratchasima province in Thailand 12. The reason why scFv fragments against
diverse targets could be identified from this non-immunized human phage display antibody library could be
because this library was created from blood of healthy volunteers who had been exposed to a wide variety of
targets within the north-eastern part of Thailand. In addition to specific scFv fragments against hazardous
mycotoxins ( Aflatoxin 13, Zearalenone 14, and Ochratoxin 15), scFv against targets with biomedical applications
have been identified by a simple but powerful whole-cell biopanning methods. These targets include
pathogenic bacteria 16 and cancer cell surface 17. Preliminary antibody engineering studies have shown that
while it is possible to improve the binding affinity of by random chain-shuffling technics 18, after conversion
of the scFv into different formats, the binding characteristic of the antibody varied, depending on the sequence
of each scFv 19. Interestingly, despite low binding affinity, our scFv could be successfully implemented for the
4th generation of CAR-T cell platform 20. These findings suggested that scFv from our library could be used as
affinity reagent for incorporation into myriad theragnostic platforms, after subjected to appropriate antibody
engineering techniques.
REFERENCE
1 Na-ngam, N. et al. Immune responses of selected phagotopes from monoclonal antibodies of Burkholderia pseudomallei.
Southeast Asian J Trop Med Public Health 39, 443-451 (2008).
2 Na-ngam, N. et al. Mimotope identification from monoclonal antibodies of Burkholderia pseudomallei using random peptide
phage libraries. Trans R Soc Trop Med Hyg 102 Suppl 1, S47-54, doi:S0035-9203(08)70014-2 [pii]
3 Srila, W. & Yamabhai, M. Identification of amino acid residues responsible for the binding to anti-FLAG™ M2 antibody using a
phage display combinatorial peptide library. Appl Biochem Biotechnol 171, 583-589, doi:10.1007/s12010-013-0326-8 (2013).
4 Yasamut, U. et al. Determination of a distinguished interferon gamma epitope recognized by monoclonal antibody relating to
autoantibody associated immunodeficiency. Sci Rep 12, 7608, doi:10.1038/s41598-022-11774-9 (2022).
5 Intasai, N. et al. Immunoreactivity of humanized single-chain variable fragment against its functional epitope on domain 1 of
CD147. Sci Rep 12, 6719, doi:10.1038/s41598-022-10657-3 (2022).
6 Khoushab, F., Jaruseranee, N., Tanthanuch, W. & Yamabhai, M. Formation of chitin-based nanomaterials using a chitin-binding
peptide selected by phage-display. Int J Biol Macromol 50, 1267-1274, doi:S0141-8130(12)00113-4 [pii]
7 Pruksametanan, N., Yamabhai, M. & Khawplod, P. in. 78-81 (IEEE International Conference on Nano/Molecular Medicine and
Engineering, NANOMED).
8 Vu, N. X. et al. Generation of a rabbit single-chain fragment variable (scFv) antibody for specific detection of Bradyrhizobium
sp. DOA9 in both free-living and bacteroid forms. PLoS One 12, e0179983, doi:10.1371/journal.pone.0179983 (2017).
9 Khaing, K. K. et al. Application of Recombinant Human scFv Antibody as a Powerful Tool to Monitor Nitrogen Fixing
Biofertilizer in Rice and Legume. Microbiol Spectr 9, e0209421, doi:10.1128/Spectrum.02094-21 (2021).
10 Kay, B. K., Kasanov, J. & Yamabhai, M. Screening Phage-Displayed Combinatorial Peptide Libraries. Methods 24, 240-246
(2001).
11 Khoushab, F. & Yamabhai, M. in The 2010 TechConnect World Conference and Trade Show. ((oral)).
12 Pansri, P., Jaruseranee, N., Rangnoi, K., Kristensen, P., Yamabhai, M. . A compact phage display human scFv library for selection
of antibodies to a wide variety of antigens. BMC Biotechnol 9, 6 (2009).
13 Rangnoi, K., Jaruseranee, N., O'Kennedy, R., Pansri, P. & Yamabhai, M. One-step detection of aflatoxin-B(1) using scFv-alkaline
phosphatase-fusion selected from human phage display antibody library. Mol Biotechnol 49, 240-249, doi:10.1007/s12033-011-
9398-2 (2011).
14 Sompunga, P., Pruksametanan, N., Rangnoi, K., Choowongkomon, K. & Yamabhai, M. Generation of human and rabbit
recombinant antibodies for the detection of Zearalenone by phage display antibody technology. Talanta 201, 397-405,
doi:10.1016/j.talanta.2019.04.034 (2019).
15 Pranomphon, R., Srila, W. & Yamabhai, M. Generation of recombinant scFv antibody against Ochratoxin A (OTA). Indonesian
Journal of Biotechnology 22, 107,
16 Min, T. T. & Yamabhai, M. Human Hexa-Histidine-Tagged Single-Chain Variable Fragments for Bioimaging of Bacterial
Infections. ACS Omega 6, 762-774, doi:10.1021/acsomega.0c05340 (2021).
17 Sumphanapai, T., Chester, K., Sawatnatee, S., Yeung, J. & Yamabhai, M. Targeting Acute Myeloid Cell Surface using a
Recombinant Antibody Isolated from Whole Cell Biopanning of a Phage Display Human scFv Antibody Library. Med Oncol in
press (2022).
18 Rangnoi, K., Choowongkomon, K., O'Kennedy, R., Ruker, F. & Yamabhai, M. Enhancement and Analysis of Human
Antiaflatoxin B1 (AFB1) scFv Antibody-Ligand Interaction Using Chain Shuffling. J Agric Food Chem 66, 5713-5722,
doi:10.1021/acs.jafc.8b01141 (2018).
19 Rangnoi, K. et al. Binding Characteristic of Various Antibody Formats Against Aflatoxins. ACS Omega 6, 25258-25268,
doi:10.1021/acsomega.1c03044 (2021).
20 Wutti-in, Y. et al. Development of a Novel Anti-CD19 CAR Containing a Fully Human scFv and Three Costimulatory Domains.
Front Oncol 11, doi:10.3389/fonc.2021.802876 (2022).
Invited Speaker
Regulatory mechanism of morphogen signals

by the autism-related gene cdc2-like kinase 2 (clk2)
in Xenopus neural development
Regina Putri Virgirinia1,2*, Kimiko Takebayashi-Suzuki1, Makoto Nakamura1,
Fatchiyah Fatchiyah2,3, and Atsushi Suzuki1
1
Amphibian Research Center, Graduate School of Science, Hiroshima University, Japan
2
Research Center of Smart Molecule of Natural Genetics Resource, Brawijaya University, Malang, East Java,
Indonesia
3
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia
*Corresponding author: regina.putri.bio@gmail.com
clk2 is an autism risk gene that contributes to neuronal defects in an autism model mouse. Although the
upregulation of Clk2 is highly associated with autistic phenotypes in the adult stage, its function during early
neural development is not well understood. Neural development in vertebrates is regulated by several
morphogens, such as bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs). Ventral
ectoderm differentiates into epidermis in response to BMPs, whereas BMP signaling is tightly inhibited in the
dorsal ectoderm which develops into neural tissues. We previously found that Clk2 promotes early neural
development and inhibits epidermis differentiation in Xenopus embryos. clk2 is specifically expressed in neural
tissues along the anterior-posterior axis during early Xenopus embryogenesis. When overexpressed in
ectodermal explants, Clk2 induces the expression of both anterior and posterior neural marker genes. Clk2
downregulates the level of p-Smad1/5/8 in cooperation with BMP inhibition, in addition to increasing the level
of activated MAPK together with FGF. These results suggest that Clk2 plays a role in early neural development
of Xenopus via modulation of morphogen signals such as the BMP and FGF pathways. As Clk2 showed an
overlapping substrate specificity with other Clk family members, it is possible that Clk family proteins act
together to promote neural development. We will present and discuss the role of Clk family proteins in early
neural development of Xenopus. The detailed analysis on the mechanism of Clk2-induced neural formation
will provide an insight into the molecular understanding of human autism spectrum disorders.
Oral Presentation
Cell-based evidence to the Anti-COVID-19 potential of

honeybee propolis ingredients
Renu Wadhwa1*, Durai Sundar2, Yoshiyuki Ishida3, Keiji Terao3, Sunil C. Kaul1
1
AIST-INDIA DAILAB, National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba 305-8565,
Japan
2
DAILAB, Department of Biochemical Engineering & Biotechnology,
Indian Institute of Technology (IIT)-Delhi, New Delhi 110-016, India
3
CycloChem Bio Co. Ltd., 7-4-5 Minatojima-Minamimachi, Chuo-ku, Kobe 650-0047, Japan
* renu-wadhwa@aist.go.jp
INTRODUCTION. The COVID-19 pandemic emerged in Wuhan city of China in December 2019 has taken
millions of lives globally. Caused by a new strain of SARS-CoV-2 (Severe Acute Respiratory Syndrome
Coronavirus-2, it has marked 2020-2021 with new endeavours in repurposing of existing drugs, as well as
initiation of anti-COVID-19 vaccine and drug development. Natural products, due to their general safety and
wider availability, have attracted research and public attention to identify and explore crucial ingredients that
could help mitigate COVID-19 infection. In this study, we have investigated two natural compounds from
honeybee propolis - CAPE (Caffeic acid phenethyl ester) and ARC (Artepillin C) for their anti-COVID-19
potential. Effect of these compounds on human cell surface receptors (ACE2-Angiotensin Converting
Enzyme2/Spike protein and TMPRSS2-Transmembrane Protease Serine 2) that are critical for virus infection,
and virus main protease (Mpro, essential for virus replication) was studied through molecular simulations and
in vitro experiments. METHODS. Structure-based computational analyses were performed to predict the
effect of these compounds on virus-host cell surface receptors. Cell-based experimental approaches were used
to investigate the effect of these compounds on the expression level of the target proteins and virus replication.
RESULTS AND DISCUSSION. Computational analyses revealed that whereas none of the two compounds
(CAPE and ARC) could bind to the ACE2/Spike interface, both interacted stably at the active site of ACE2 as
well as the viral protein, Mpro. ARC, but not CAPE, showed stable interaction with TMPRSS2. Human cells
treated with CAPE or ARC showed downregulation of both the receptors. Furthermore, cell- and PCR-based
SARS-CoV-2 replication assays endorsed their antiviral activity in which CAPE caused higher inhibition than
ARC. CONCLUSION. The findings suggest that the honeybee propolis-derived compounds, CAPE and ARC,
could be helpful in the reduction of viral replication/infection, and hence warrant further experimental and
clinical studies.
…..
Oral Presentation
The Effect of Serial Dilution on Viability 3T3-L1 Cell Line

Cicin Vinolia Alvionita1 and Fatchiyah Fatchiyah1,2*
1
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, East Java,
65145, Indonesia
2
Research Center of Smart Molecule of Natural Genetics Resources, Brawijaya University, Malang, East Java, 65145,
Indonesia
*Corresponding author: Fatchiyah Fatchiyah; e-mail: fatchiya@ub.ac.id ; tel: +62-341-575841
INTRODUCTION. 3T3-L1 is the cell line that has a morphology like a fibroblast, but under appropriate
conditions, the cells differentiate into a phenotype like an adipocyte. 3T3-L1 is isolated from murine
preadipocytes that are widely used in studies related to adipogenesis such as dyslipidemia, obesity, diabetes,
and related correlation on aging cells. This study aims to determine the effect of the viability of 3T3-L1 cells
with serial dilutions, so that they can be used as a reference for preliminary studies. METHODS. This study
used cell lines 3T3-L1 which had passed two passaging to stabilize the growth of cell lines. Cells were cultured
in flasks until they were about 70% - 80% confluent. Confluent cells 3T3-L1 were serially diluted in 24 wells
culture, Which was divided into 3 parts so that each part had 8 culture wells. This method was designed for
measurements at 24, 48, and 72 hours. RESULTS AND DISCUSSION. The results showed that serial dilution
affected the viability of the 3T3-L1 cultured cells. The graph has decreased from 24 hour to 72 hour.
CONCLUSION. Conclusion of analysis shows that there is an influence between cell density and media
volume in a culture well which causes a decrease in viability over time.
Serial dilution
3T3-L1 cell line
Graphical result
Counting viability
REFERENCE
[1] Mehra, A.; Macdonald, I.; Pillay, T. S. Variability in 3T3-L1 adipocyte Differentiation Depending on Cell Culture Dish. Analytical
biochemistry, 362(2) : 281-283(2007).
[2] Stoddart, M. J. Cell Viability Assays: Introduction. Mammalian cell viability. 1-6 (2011).
[3] Tsai, C. Y.; Peh, M. T.; Feng, W.; Dymock, B. W.; Moore, P. K. Hydrogen Sulfide Promotes Adipogenesis in 3T3L1 Cells. PLoS
One, 10(3) : 1 – 16 (2015).
[4] Dong, S. F.; Yasui, N.; Negishi, H.; Kishimoto, A.; Sun, J. N.; Ikeda, K. Increased Oxidative Stress in Cultured 3T3-L1 Cells
Was Attenuated by Berberine Treatment. Natural product communications. 10(6) : 895 – 897 (2015).
Oral Presentation
Color Decoration of Chicken Egg Using Natural Dye Molecule

in Sappan Wood
Tutik Setianingsih1*, Danar Purwonugroho1, Yuniar Ponco Prananto1, Afif Eka Rahma
Setiyanto2, Adi Kurnia Soesantyo1, Silvi Zakiyatul Ilmiyah2, Kashifa Maria Jihan1
1
Department of Chemistry, Brawijaya University, Malang; danar@ub.ac.id; prananto@ub.ac.id,
stevinoadi20@student.ub.ac.id; kashifamj02@student.ub.ac.id
2
Department of Biology, Brawijaya University, Malang; afarato@gmail.com; silvi_zakiya@student.ub.ac.id
*coresponding author: tutiksetia@ub.ac.id
Chicken egg is one of popular foods in Malang city. This animal product is rich of nutrients such protein,
fat, various amino acids, minerals, and vitamins [1]. Decoration of egg has been introduced but still one
color (brown) and no pattern of decoration, such as Bacem of Jogja. The other various colors of the
decorated eggs have been made but still using the synthetic food dyes so that no addition of nutrition or
antioxidant. In our community service 2022 by DPP-SPP funding, colorization of the chicken egg by
involving the patterned decoration art was introduced by using sappan wood. This natural product contains
brazilein dyes molecules [2,3]. Decoration process included the boiling of egg, the patterned peeling of the
egg shell, re-boiling of the decorated egg with addition of sappan wood and spices, immersion of the
decorated egg in the mixture, and finally peelling of all colorized egg shells. The different color decoration
of the eggs as result of different conditions (spice composition and different concentration of dyes) were
observed. The molecular chemical interactions which are involved in color decoration of the eggs at the
different conditions were predicted.
REFERENCES:
[1] Wulandari, Z dan Arief, I.I., Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan, 10(2), 62-68 (2022)
[2] Sari R. dan Suhartati, Teknis Eboni, 13 (1), 57 - 67 (2016)
[3] Sari, DY, Widiantoro, A, Alimuddin, AH, 3(1),1-15(2018)
Oral Presentation
IN SILICO STUDY OF BIOACTIVE COMPOUNDS FROM

TANGERINES (CITRUS RETICULATA) AS PROTEIN
INHIBITOR FOR 3CLPRO SARS-COV-2 : OMICRON
VARIANT
Victor Alvianoes Guterez Hose1*, Shella Zahra Kumala Azmi1, Muhammad Faiz Maulana1,
Daniel Tumbur Hamonangan Sirait1, Ailsa Calista Salsabila1, Rahmat Grahadi2 and
Fatchiyah Fatchiyah2*
1
Department of Biology, Faculty of Mathematics and Natural Science, Brawijaya University
(Jalan Veteran, Malang, East Java, Indonesia, 65145)
2
Research Center of Smart Molecules of Natural Genetic Resources (SMONAGENES), Brawijaya University
(Malang, East Java, Indonesia)
COVID-19 is a disease caused by coronavirus that was identified as a new strain of coronavirus last December
2019. Coronavirus disease can be handled by blocking its protein using bioactive compounds, it is known that
there are compounds from Citrus reticulata that have potential as antiviral. An in silico evaluation of the active
compounds available in Citrus reticulata was carried out to compare their anti-inflammatory effects based on
the molecular characteristics of docking with nirmatrelvir as a control compound. In addition, analysis of
binding affinity, binding site, ADME prediction, and molecular dynamics simulation was also carried out. The
active compounds in Citrus reticulata were collected from the PubChem database tested on the Way2Drug
website with the AntiVir-Pred service. The docking process had done with Autodock Vina. The results
obtained indicate that neohesperidin compounds can bind to receptors that bind to catalytic sites on Cys145
and His41 as well as nirmatrelvir. In addition, there is also phlorin which can bind to the catalytic site of the
His41 receptor. Active site analysis of the interaction suggests neohesperidin and phlorin compounds may
have a therapeutic effect against COVID-19. However, from the results of ADME predictions, not all of these
compounds qualify Lipinski's rule criteria such as neohesperidin and diosmin.
Oral Presentation
The Effect of Black Cumin Extract in Self Nanoemulsifying

Drug Delivery System (Snedds) Preparation On Renal
Histopathology After Diabetes Mellitus Induction With
Alloxan
Dinda Nawang Sari1*, Syaefudin Ali Akhmad2
1
Undergraduate Student, Faculty of Medicine, Universitas Islam Indonesia
2
Department of Biochemistry, Faculty of Medicine, Universitas Islam Indonesia
*Corresponding author: dindanawang@gmail.com
INTRODUCTION. The disease Diabetes Mellitus (DM) is one of the major public health problems1. The
objective of therapy in DM by continuously controlling blood sugar level is to prevent further complications,
such as nephropathy which causes highest morbidity in patients2. Black cumin seed oil is well acknowledged
to contain anti-diabetic properties3. The utilization of black cumin in the form of SNEDDS complemented by
its high bioavailability is envisaged to elevate the therapeutic effect with low toxicity in people with DM4. This
study aims to determine the effectiveness of black cumin ethanol extract in the form of SNEDDS on the
histological structure of rats' kidneys after diabetes induction with alloxan. METHODS. Researchers apply
the “true experimental” and a post-test control group research design by utilizing male Rattus norvegicus rats
which are then categorized into seven groups. The experimental group rats are induced with diabetes by
administering alloxan (150 mg/kgBW, intraperitoneally) and intervened for 4 weeks. The control group rats
are administered with glibenclamide and placebo. The treatment group rats are administered with black cumin
seed extract (200 mg/kgBW and 400 mg/kgBW) as well as black cumin seed extract in the form of SNEDDS
(200 mg/kgBW and 400 mg/kgBW). Subsequently, the rats' kidneys undergo histological examination.
Ultimately, the data attained is analyzed by employing SPSS 24 statistical software. RESULTS AND
DISCUSSION. The histological observation demonstrates the presence of tubular narrowing, necrosis cells,
and increased diameter of the glomerulus. The analytical results demonstrate no significant difference between
the group of (p value>0.05) and (p=0.091) for tubular narrowing, (p=0.067) for necrosis cells, and (p=0.526)
for glomerulus diameter. CONCLUSION. Black cumin seed extract in the form of SNEDDS does not
significantly affect the histological changes of rats’ kidneys.
REFERENCE
[1] WHO. GLOBAL REPORT ON DIABETES. 2016. Retrieved from https://www.who.int/publications/i/item/9789241565257
[2] Tridjaja B, Yati NP, Faizi M, Marzuki N, Moelyo AG, Soesanti F. Konsensus Nasional pengelolaan diabetes mellitus tipe 1.
UKK Endokrinologi Anak dan Remaja; 2015.
[3] Younus H, Sawhney. Molecular and Therapeutic: Actions of Thymoquinone. Younus H, editor. Berlin/Heidelberg, Germany:
Springer; 2018.
[4] Baloch J, Sohail MF, Sarwar HS, Kiani MH, Khan GM, Jahan S, Rafay M, Chaudhry MT, Yasinzai M, Shahnaz G. Self-
nanoemulsifying drug delivery system (SNEDDS) for improved oral bioavailability of chlorpromazine: in vitro and in vivo
evaluation. Medicina. 2019 May;55(5):210.
Oral Presentation
The Potential of Ferulic Acid as an Anti-virulence to Combat

Pathogenic Bacteria
Ernanin Dyah Wijayanti1,2,3, Anna Safitri2,4, Dian Siswanto1, Lidwina Faraline Triprisila2,5,
and Fatchiyah Fatchiyah1,2*
1
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya
2
Research Center of Smart Molecule of Natural Genetics Resources, Universitas Brawijaya
3
Academy of Pharmacy of Putra Indonesia Malang
4
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya
5
Biosains Institute, Universitas Brawijaya
INTRODUCTION. Pathogenic bacteria contain virulence factors, including lipoproteins (LPP),

lipopolysaccharides (LPS), and flagellins. They are recognized by toll-like receptors (TLRs) that allow bacteria
to enter the cells, causing infection and inflammation. Therefore, anti-virulence strategies could be applied as
a new alternative target for antimicrobial therapy. Ferulic acid (FA) has been known for its antimicrobial and
anti-inflammatory properties, but the mechanism for preventing bacterial virulence factors recognition has not
been elucidated yet. This study aimed to determine the potential of FA as anti-virulence factor for bacteria by
in silico study. METHODS. The interactions among FA (CID 445858), LPP (2RA2), LPS (3VQ2) flagellin
(1IO1), TLR2 (6NIG), TLR4 (2Z63), and TLR5 (3J0A) were docked by Hex 8.0.0 Cuda, then visualized by
Discovery Studio 2020 and LigPlot. RESULTS AND DISCUSSION. FA could bind to all virulence factors
and TLRs in some amino acid residues. Interaction of FA to TLRs-bacterial virulence factors complexes
produces higher binding energies than the interaction of TLRs-bacterial virulence factors only. FA reduces the
binding of TLR2-LPP, TLR4-LPS, and TLR5-flagellins complexes and inhibits recognizing bacterial
virulence factors by binding to TLRs. CONCLUSION. These remarkable findings suggest that FA could
prevent bacterial pathogenesis and thus could be employed as an anti-virulence therapeutic strategy.
REFERENCE
[1] Wijayanti, Ernanin Dyah; Safitri, Anna; Siswanto, Dian; Triprisila, Lidwina Faraline; and Fatchiyah, Fatchiyah, Makara Journal of
Science, 25, 4 (2021).
Oral Presentation
In Silico and ADMET Analysis of Potential Compounds in

Licorice Root (Glycyrrhiza glabra) as PPAR-γ Activator to
Increase Insulin Sensitivity in Type 2 Diabetes Mellitus
Maurin Ardhina Syafiudin1,2, Firda Nuri Asyhari1, Atika Mahardika Indriani1, Faizah
Nurhabibah1, Puteri Adila Azizah1, and Nina Regina Nathania1,2, Nia Kurniawan1, Fatchiyah
Fatchiyah1,2
1
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jalan Veteran, Malang,
East Java, Indonesia, 65145
2
Research center of SMONAGENES, Brawijaya University, Jalan Veteran, Malang, East Java, Indonesia, 65145
*Corresponding author: Fatchiyah Fatchiyah e-mail: fatchiya@ub.ac.id

*
Diabetes mellitus type 2 is characterized by normal or increased insulin secretion, but reduced target cell
sensitivity to insulin. Insulin resistance or insulin secretion damage is the cause of type 2 diabetes. Diabetes
today affects 5% of the global population, and its incidence is rapidly increasing, particularly among the
elderly. One medication or method for reducing diabetes mellitus type 2 is by increasing the sensitivity of the
insulin receptor, namely PPAR-ɣ. Thiozolididinedione as a PPAR-ɣ agonist is useful in the regulation of
diabetes by increasing insulin sensitivity. Several secondary metabolites of licorice (G. glabra) have many
pharmacological benefits, such as antidiabetic. This study aims to determine the potential of licorice compound
(G. glabra) for type 2 diabetes mellitus by increasing insulin sensitivity. This research use PPAR-ɣ receptor
and Glycycoumarin, glycyrin, glyasperin D, dehidrogenylasperin C, glyasperin B, isoglycyrol, glycyrrhetinic
acid, glabridin, glabrene, isoliquiritigenin and liquiritigenin as ligands. This research is done by molecular
docking & visualizations, ADME, and toxicity predictions. Compounds that have the best bond are Glycerin
B and Isoliquiritigenin. Isoliquiritigenin has great ADME and drug-like properties. Isoliquiritigenin is the best
compound that can be chosen as a drug material for insulin sensitizer based on molecular docking, ADME,
and toxicity analysis.
REFERENCE
[1] Goldstein BJ & Wieland-Müller D., Type 2 Diabetes: Principles and Practice, 2016, CRC Press, Florida.
[2] Sherwood L. Introduction to Human Physiology. Cengage Learning. 2016. New York.
[3] Arif R, Ahmad S, Ghulam M, Mahrosh HS, Ali M, et al. Molecular Docking and Simulation Studies of Antidiabetic Agents Devised from
Hypoglycemic Polypeptide-P of Momordica charantia. BioMed Research International. 2021. 5561129: 1-15.
[4] Selvaraj G, Kaliamurthi S, & Thirugnanana-sambandam, R. Molecular docking studies on potential PPAR-γ agonist from Rhizophora apiculate.
Bangladesh J Pharmacol. 2014. 2014(9): 298-302.
[5] Hasan MK, Ara I, Mondal MSA, & Kabir, Y. Phytochemistry, pharmacological activity, and potential health benefits of Glycyrrhiza glabra.
Heliyon. 2021. e07240 (2021): 1-10.
[6] Pratama, M. R. F. & T. Gusdinar. Docking study of secondary metabolites from Glycyrrhiza glabra as PPAR-γ agonist. Biointerface Research
in Applied Chemistry. 2019. 9(4): 4006-4010.
[7] Saputri, K, E., N. Fakhmi, E. Kusumaningtyas, D. Priyatama, & B. Santoso. Docking Molekular Potensi Anti Diabetes Melitus Tipe 2
Turunan Zerumbon Sebagai Inhibitor Aldosa Reduktase Dengan Autodock-Vina. Chimica et Natura Acta. 2016. Vol. 4(1):16-20.
[8] Xue, Q., X. Liu, P. Russell, J. Li, W. Pan, J. Fu, & A. Zhang. Evaluation of the binding performance of flavonoids to estrogen receptor alpha
by Autodock, Autodock Vina and Surflex-Dock. Ecotoxicology and Environmental Safety. 2022. 233: 1-7
[9] Bhat, V., & Chatterjee, J. The Use of In Silico Tools for the Toxicity Prediction of Potential Inhibitors of SARS-CoV-2. Alternatives to
laboratory animals: ATLA. 2021. 49(1-2): 22–32.
[10] Sharif, M. A., I. Tsakovska, P. Alov, V. Vitcheva, A. Diukendjieva, & I. Pajeva. Molecular Modeling Approach to Study the PPARγ–
Ligand Interactions. Methods in Molecular Biology. 2019. Vol. 1996:261-289.
[11] Guasch, L., E. Sala. A. Castell-Auvi, L. Cedo, K.R. Liedl, G. Wolber, et all. Identification of PPARɣ Partial Agonists of Natural Origin (I):
Development of a Virtual Screening Procedure and In Vitro Validation. PLoS One. 2012. Vol. 7(11):1-13.
[12] Kai, QXA., Rumengan IFM., Lintang RAJ, Wullur S, Sumilat DA, Pankey H, & Luntungan HA. Penambatan Molekul Glutation Fauna Laut
Terhadap Reseptor dari Beberapa Penyakit Virus. J Pesisir dan Laut Tropis. 2021. Vol. 9 (2):53-58.
[13] Amano, Y. T. Yamaguchi, K. Ohno, T. Niimi, M. Prita, H. Sakashita & M. Takeuchi. Structural basis for telmisartan-mediated partial
activation of PPAR ɣ. Hypertension Research. 2012. Vol. 35:715-119
[14] Olusola OE, Oluwamodupe CE, Jean PK, Oluwaseun BA, Ayodeji F, Damilare DA, et al. 2018. Discovery of potential visfatin activators using in
silico docking and ADME predictions as therapy for type 2 diabetes. J. of Basic and Applied Sci. 7 (2018): 241-249.
[15] Bucao XEN & Judilynn NS. 2021. In Silico Evaluation of Antidiabetic Activity and ADMET Prediction of Compounds from Musa acuminata
Colla Peel. Philippine J. of Sci. 151 (1): 171-192.
[16] Abdi B, Fekadu M, Zeleke D, Eswaramoorthy R, Melaku Y. Synthesis and Evaluation of the Antibacterial and Antioxidant Activities of Some
Novel Chloroquinon Analogs. Journal of Chemistry. 2021. 2021(2408006): 1-13.
Oral Presentation
Comparison of The Effect Between Black Cumin (Nigella

sativa) in Form of Self-Nanoemulsifying Drug Delivery System
(SNEDDS) and Non-SNEDDS on The Number of
Hippocampus Pyramid Cells of Alloxan-Induced Diabetic
Rats (Rattus Norvegicus)
Muhammad Zenryu Asmara1, Syaefudin Ali Akhmad2
1
2
Email: 1zenryua@gmail.com
INTRODUCTION: Nigella sativa is acknowledged as an alternative treatment for Diabetes Mellitus (DM)
and prevent organ damage due to its complication. The use of Nigella sativa which is insoluble in water has
several limitations, especially in absorption if administrated via oral route. Self-Nanoemulsifying Drug
Delivery System (SNEDDS) was developed to increase the bioavailability of molecules that are difficult to
dissolve in water. The administration of Nigella sativa in the form of SNEDDS is expected to provide better
effects than non-SNEDDS form. Objective: The purpose of this study was to compare the effect of Nigella
sativa in form of SNEDDS with the non-SNEDDS form on the number of hippocampus pyramid cells of Rattus
norvegicus after the induction of Diabetes mellitus with alloxan. METHODS: The method used is a true-
experimental study using 35 male rats divided into 7 groups: K+F (physiological positive); K+P (glibenclamide
5 mg/kgBW); K- (placebo); P1 (non-SNEDDS Nigella Sativa (200 mg/kgBW)); P2 (non-SNEDDS Nigella
sativa (400 mg/kgBW)); P3 (SNEDDS Nigella sativa (200 mg/kgBW)) and P4 (SNEDDS Nigella sativa (400
mg/kgBW)). The intervention group induced diabetes using 150 mg/KgBW of alloxan via intraperitoneal
injection. The intervention was administered orally for 30 days. Calculation of the number of hippocampus
pyramid cells was carried out by making hematoxylin-eosin (HE) preparations and observed under a
microscope. Data were analyzed using one way ANOVA. RESULTS AND DISCUSSION: The results of
this study showed that there was no significant difference in the number of hippocampus pyramid cells between
Nigella sativa in form of SNEDDS and non-SNEDDS (P > 0.05). The mean number of hippocampus pyramid
cell were K+F (42,68), K+P (46,04), K- (40,24), P1 (42,84), P2 (40,64), P3 (41,16), P4 (41,80). The
discrepancy between the results and the hypothesis was caused by the induction of diabetes using alloxan so
the blood sugar levels were unstable. Furthermore, the 30 days of intervention was not long enough to affect
the brain. CONCLUSION: The effect of Nigella sativa in form of SNEDDS is not better than Nigella sativa
without SNEDDS on the number of pyramidal cells of the hippocampus.
Keywords: Black cumin, Nigella sativa, Diabetes mellitus, SNEDDS, Hippocampus.
Oral Presentation
Computational Insight on Potential Activity of Star Apple

(Chrysophyllum cainito L.) Bioactive Compound as PPAR-γ
Antagonist for Obesity
Nabila Nur Rosyada1,2, Regina Shania Andyni1,2 , Cicin Vinolia Alvionita1,2 , Sekararum
Narwasthu1,2 , Robiatul Adawiyah1, Feri Eko Hermanto1, Nia Kurniawan1 and Fatchiyah
Fatchiyah1,2*
1
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang 65145,
Indonesia
2
Research Center of Smart Molecule of Natural Genetics Resources, Universitas Brawijaya, Malang 65145, Indonesia
INTRODUCTION. Studies reported that star apple (C. cainito) has the potential as an anti-obesity agent.
However, the mechanism underlying that activity remains unknown. on the other hand, targeting PPAR-γ has
played a beneficial role in regulating obesity. Therefore, this study will explain the anti-obesity potential of
natural compounds from C. cainito in modulating obesity through inhibition of PPAR-γ. METHODS.
Molecular docking was employed to identify the possibility of several compounds binding with the PPAR-γ.
Moreover, the stability of the interaction between PPAR-γ and each compound also was evaluated using
molecular dynamics analysis. RESULTS AND DISCUSSION. The result showed that β-sitosterol, lupeol,
myricitrin, quercitrin, rutin, vitamin A, epicatechin, and chlorogenic acid are worthy of potential as PPAR-γ
antagonists. In advance, molecular dynamics simulations revealed that chlorogenic acid, quercitrin, and
epicatechin were the most stable compound to interact as an inhibitor of PPAR-γ. CONCLUSION. In
conclusion, compounds from star apple have some potential to improve the health status of patients’ obesity
through PPAR-γ antagonist activity.
REFERENCE
[1] M.I. Cardel, M.A. Atkinson, E.M. Taveras, J.-C. Holm, A.S. Kelly, Obesity Treatment Among Adolescents: A Review of Current Evidence and
Future Directions, JAMA Pediatr. 174 (2020) 609–617. https://doi.org/10.1001/jamapediatrics.2020.0085.
[2] M.L. Endalifer, G. Diress, Epidemiology, Predisposing Factors, Biomarkers, and Prevention Mechanism of Obesity: A Systematic Review,
Journal of Obesity. 2020 (2020) e6134362. https://doi.org/10.1155/2020/6134362.
[3] N. Sangaji, V.H. Wiyono, T. Mulyaningsih, Socio-Economic Determinants of Obesity in Indonesia: Analysis of IFLS 2014 Data, Budapest
International Research and Critics Institute (BIRCI-Journal): Humanities and Social Sciences. 5 (2022) 1939–1947.
https://doi.org/10.33258/birci.v5i1.3794.
[4] V.S. Malik, W.C. Willet, F.B. Hu, Nearly a decade on — trends, risk factors and policy implications in global obesity, Nat Rev Endocrinol. 16
(2020) 615–616. https://doi.org/10.1038/s41574-020-00411-y.
[5] S.P. Sharma, H.J. Chung, H.J. Kim, S.T. Hong, Paradoxical Effects of Fruit on Obesity, Nutrients. 8 (2016) 633.
https://doi.org/10.3390/nu8100633.
[6] S. Alinia, O. Hels, I. Tetens, The potential association between fruit intake and body weight--a review, Obes Rev. 10 (2009) 639–647.
https://doi.org/10.1111/j.1467-789X.2009.00582.x.
[7] L. Hebden, F. O’Leary, A. Rangan, E. Singgih Lie, V. Hirani, M. Allman-Farinelli, Fruit consumption and adiposity status in adults: A systematic
review of current evidence, Crit Rev Food Sci Nutr. 57 (2017) 2526–2540. https://doi.org/10.1080/10408398.2015.1012290.
[8] S.J. Guyenet, Impact of Whole, Fresh Fruit Consumption on Energy Intake and Adiposity: A Systematic Review, Frontiers in Nutrition. 6 (2019).
https://www.frontiersin.org/article/10.3389/fnut.2019.00066 (accessed May 17, 2022).
[9] H.V. Doan, T.P. Le, Chrysophyllum cainito: A Tropical Fruit with Multiple Health Benefits, Evidence-Based Complementary and Alternative
Medicine. 2020 (2020) e7259267. https://doi.org/10.1155/2020/7259267.
Oral Presentation
Virtual Based Screening of Rosmarininc Acid and its

Derivatives as Potential Anticancer Approach Targeting HIF-
1α
Achmad Hanif Naufal1, Nisa Nabila Aufa Wachid1, Fairuz Sarah Kamila1, Nadia
Wahyuningsih1, Silvi Zakiyatul Ilmiyah1, Wirdatun Nafisah2, Nia Kurniawan3 and Fatchiyah
Fatchiyah3*
1
Undergraduate Program, Departemen of Biology, Faculty of Mathematics and Natural Science, Brawijaya University
2
Doctoral Program, Departemen of Biology, Faculty of Mathematics and Natural Science, Brawijaya University
3
Departemen of Biology, Faculty of Mathematics and Natural Science, Brawijaya University
INTRODUCTION. Cancer is one of the leading causes of death in the world. Rosmarinic acid is an essential
polyphenolic compound and includes derivatives of two amino acids, namely caffeic acid from phenylalanine
and 3,4-dihydroxy phenyl-lactic acid from tyrosine, secondary metabolites found in many herbal plants from
the subfamily Lamiaceae and Boraginaceae. Rosmarinic acid works in inhibiting the occurrence of metastases
and tumor formation. METHODS. In this study, we evaluated rosmarinic acid and its derivatives through
study literature. The compounds were downloaded from the PubChem database, while the three-dimensional
structure of hif-1ɑ was retrieved from RCSB PDB. The biological activity was analyzed using the PASS server
and the cytotoxic effect was predicted using CLC-Pred. The pharmacological properties of bioactive
compounds were analyzed using SwissADME. The compounds and hif-1ɑ were prepared using PyRx 0.8
version and Discovery Studio software, docked specifically using Autodock Vina integrated with PyRx 0.8,
visualized using Discovery Studio software, and molecular dynamic simulation using CABS-flex 2.0.
RESULTS AND DISCUSSION. Rosmarinic acid and its derivative showed an inhibition with the highest
affinity value of compound against hif-1α are control hif-1α (-6.9 kcal/mol), rosmarinic acid (-6.4 kcal/mol),
methyl rosmarinate (-6.3 kcal/mol), and 3-O-methylrosmarinic acid (-6 kcal/mol). Residues interaction
residues of the four compounds bind to hif-1α at the PasB domain site, where the PasB domain is crucial for
establishing protein-protein interactions with hif-1β. The PasB domain of important in ‘scaffolding’ the
complex. CONCLUSION. Dimerization of hif-1α and hif-1β cause further complications in metastases or
cancer and rosmarinic acid is predicted as an inhibitor of hif-1α and hif-1β dimerization. Comprehensive and
further analysis is required in future research based in vivo and in vitro for the development of cancer
treatments.
Keywords: cancer, HIF-1ɑ, Rosmarinic acid, ADME, docking
REFERENCE
[1] Groot, P. M.; Wu, C. C.; Carter, B. W.; Munden, R. F. The epidemiology of lung cancer. Translational Lung Cancer Research.
2018, 7, 220–233, https://doi.org/10.21037/tlcr.2018.05.06.
[2] Imani, A. Maleki, N. Bohlouli, S. Kouhsoltani, M. Sharifi, S. Dizaj, S. M. Molecular mechanisms of anticancer effect of rutin.
Phytotherapy Research. 2021, 35: 2500-2513, https://doi.org/10.1002/ptr.6977
[3] Song, Y.; Ye, M.; Zhou, J.; Wang, Z.; Zhu, X. Targeting E–cadherin expression with small molecules for digestive cancer
treatment. J. Am. Transl. Res. 2019, 11, 3932–3944.
[4] Burslem, G. M.; Kyle, H. F.; Nelson, A.; Edwards, T. A.; & Wilson, A. J. Hypoxia inducible factor (HIF) as a model for studying
inhibition of protein-protein interactions. Chemical science. 2017, 8(6), 4188–4202. https://doi.org/10.1039/c7sc00388a.
[5] Wilkins, S. E.; Abboud, M. I.; Hancock, R. L.; & Schofield, C. J. Targeting Protein-Protein Interactions in the HIF
System. ChemMedChem. 2016, 11(8), 773–786. https://doi.org/10.1002/cmdc.201600012.
[6] Poyya, J.; Joshi, C. G.; Kumar, D. J.; & Nagendra, H. G. Sequence Analysis and Phylogenetic Studies of Hypoxia-Inducible
Factor-1α. Cancer informatics. 2017, 16, 1176935117712242. https://doi.org/10.1177/1176935117712242.
[7] Hu, Y.; Romão, E.; Vincke, C.; Brys, L.; Elkrim, Y.; Vandevenne, M.; Liu, C.; & Muyldermans, S. Intrabody Targeting HIF-1α
Mediates Transcriptional Downregulation of Target Genes Related to Solid Tumors. International journal of molecular sciences.
2021, 22(22), 12335. https://doi.org/10.3390/ijms222212335.
Oral Presentation
The Potential of Bioactive Compounds from Cyperus rotundus

as First Aid Medication on Snakebite Envenomation by
Bungarus candidus: In-silico Study
Rahmat Grahadi1, Nia Kurniawan2, and Fatchiyah Fatchiyah3*
1
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang,
Indonesia, 65145; Research Center of Smart Molecules of Natural Genetic Resources (SMONAGENES), Brawijaya
University, Malang, Indonesia. Email: rahmat@student.ub.ac.id
2
Indonesia, 65145. Email: wawan@ub.ac.id
3
Indonesia, 65145; Research Center of Smart Molecules of Natural Genetic Resources (SMONAGENES), Brawijaya
University, Malang, Indonesia. Email: fatchiya@ub.ac.id
INTRODUCTION. Bungarus candidus is one of the most venomous snakes in the Southeast Asia. This snake
primarily inhabits rice fields and cause several mortality cases around that area. Proper first aid management
may prevent the victim from death. Therefore, we analyze the potential of bioactive compounds of the Cyperus
rotundus to block major neurotoxin of the Bungarus candidus as alternative medication for the first aid
management. METHODS. Twenty one bioactive compounds of the Cyperus rotundus were screened using
SwissADME with Lipinski parameter and lipophilicity. We analyzed the interactions between bioactive
compounds and the α-Bungarotoxin using molecular docking using Autodock Vina on PyRx v8.0. RESULTS
AND DISCUSSION. From 21 bioactive compounds of the Cyperus rotundus, 19 of them were showing
druglikeness characteristic that met lipinski parameters. Five of the bioactive compounds namely, Cyperol,
Cyperusol B2, Rotundine A, Rotundine B, and 4,6,3’,4’ Tetramethoxyaurone has highest lipophilicity value.
These bioactive compounds were docked and can bind into active site of the α-Bungarotoxin. The lipophilicity
and docking results showed that these 5 bioactive compounds could cross phospolipid membrane and then
delivered to lymphatic system to block one of the Bungarus candidus major toxin. CONCLUSION. The five
bioactive compounds of the Cyperus rotundus showed the potential to be alternative medication to help first
aid management for the Bungarus candidus envenomation.
REFERENCE
[1] Hemanth Kumar K, Razack S, Nallamuthu I, Khanum F. Ind Crops Prod. 2014;52:815–26.
[2] Scherf T, Kasher R, Balass M, Fridkin M, Fuchs S, Katchalski-Katzir E. Proc Natl Acad Sci U S A. 2001;98(12):6629–34.
[3] Daina A, Michielin O, Zoete V. Sci Rep. 2017;7(March):1–13.
Oral Presentation
Difference Effect Between Black Cumin Seeds Extract (Nigella

Sativa) in The Form of Self-Nanoemulsifying Drug Delivery
System (SNEDDS) and Non-SNEDDS on The Histopathology
of Hepar of Alloxan-Induced Diabetic Rattus Norvegicus: An
Experimental Study
Rulianty Febriani1, Syaefudin Ali Akhmad2
1
Sleman, Special Region of Yogyakarta, Indonesia, Universitas Islam Indonesia, rulianti.feby@gmail.com
2
Sleman, Special Region of Yogyakarta, Indonesia, Universitas Islam Indonesia, aliakhmad@uii.ac.id
*Corresponding author: rulianti.feby@gmail.com
INTRODUCTION. As hepatoprotector and antidiabetic1, Nigella sativa is acknowledged to prevent organ

damage due to complication of Diabetes Mellitus (DM), a metabolic disease which causes complications in
various organs, especially in the liver. The administration of Nigella sativa formulated in the form of SNEDDS
is expected to provide better therapeutic effects than non-SNEDDS form. The aim of the study was to
comprehend the effect of Nigella sativa’s extraction in SNEDDS form compared to its extraction without
SNEDDS on the histopathological changes of Rattus norvegicus’ liver after the induction of type I DM with
alloxan. METHODS. Researchers use 35 male rats categorized into seven groups: Physiological positive
(K+F); pathological K+ (glibenclamide); K- (placebo); P1 (non-SNEDDS Nigella sativa, 200 mg/kgBW); P2
(non-SNEDDS Nigella sativa, 400 mg/kgBW), P3 (SNEDDS Nigella sativa, 200 mg/kgBW) and P4
(SNEDDS Nigella sativa, 400 mg/kgBW). Diabetes was induced in the intervention groups via intraperitoneal
injection of alloxan (150 mg/kgBW) and intervened for 4 weeks. Subsequently, the result of histopathological
examination of the liver is analyzed by the Kruskal Wallis test with SPSS 24. RESULTS AND
DISCUSSION. Histopathological examination demonstrates that damage occurs to hepatocyte cells in the
form of ballooning degeneration in all groups. Bar chart below shows that the second highest cell damage
occurred in the P3 group which is inconsistent with the research objectives. This study finds that the
administration of Nigella sativa formulated in the form of SNEDDS and non-SNEDDS has no significant
difference (p>0.05). The failure of this study is prompted by a procedural error during the administration of
alloxan, which eventually affects its successful performance in rats’ bodies. Furthermore, the unmeasured level
of thymoquinone administration also contributes to the failure of this study. As a comparison, Abdellatif (2013)
has confirmed that the administration of 29.7% of thymoquinone succeeded in reversing the changes in the
liver of rats induced by DM. CONCLUSION. The administration of black cumin extract formulated in the
form of SNEDDS and non-SNEDDS has no significant effect on repairing liver histological damage in rats
induced by type I DM with alloxan.
Average score in each group

3
Scoring Result
0
K+F K+P K- P1 P2 P3 P4
Group
REFERENCE
[1] Mathur. Antidiabetic Properties of a Spice Plant Nigella sativa. Journal of Endocrinology and Metabolism (2011).
[2] Abdellatif, N. A. Protective Effect of Nigella Sativa Against Diabetic Complications on the Liver in White Male Rats. The Egyptian Journal of
Hospital Medicine (2013).
Oral Presentation
In Silico Approach of Potential Compounds in Tomato

(Solanum Lycopersicum) as Antidiabetic
Sekararum Narwasthu1,2 and Fatchiyah Fatchiyah1,2*
1
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, East Java,
65145, Indonesia
2
Research Center of Smart Molecule of Natural Genetics Resources, Brawijaya University, Malang, East Java, 65145,
Indonesia
*Corresponding author: Fatchiyah Fatchiyah; e-mail: fatchiya@ub.ac.id; tel: +62-341-575841
INTRODUCTION. Diabetes mellitus is a chronic disorder caused by lack of insulin produced by the pancreas.
Insulin is a hormone that regulates blood sugar or glucose. In the process, there is an immunoglobulin receptor
that is often studied in the context of diabetes, namely RAGE which will bind to hyperglycaemic and pro-
inflammatory biomarkers, resulting from the non-enzymatic glycation of protein and sugar, namely Advanced
glycation end products (AGEs). Previous studies have shown that tomato (Solanum lycopersicum) has potential
as an antidiabetic carried out in vivo and in vitro. However, the mechanism of tomato activity as an antidiabetic
carried out in silico is still not clearly known. Therefore, this study explains the potential of natural compounds
in tomatoes as antidiabetic through in silico analysis. METHODS. AGEs and potential compounds in
tomatoes were taken from the PubChem database and RAGE was taken from the RCSB PDB database in 3D.
Molecular docking is used to analyze several natural compounds that bind to protein targets using AutoDock
Vina software and visualized using Discovery Studio. RESULTS AND DISCUSSION. The results showed
that Apigenin and Naringenin could inhibit the AGEs-RAGE signaling pathway. Complex interaction between
RAGE-Naringenin-Pentosidine, RAGE-Naringenin-MOLD, RAGE-Apigenin-Pentosidine, RAGE-Apigenin-
MOLD with binding affinity of -6.4 kcal/mol, -5.6 kcal/mol, -5.9 kcal/mol, -5.7 kcal/mol, respectively. The
three compounds in tomatoes block RAGE activation by binding to the V domains. CONCLUSION. In
conclusion, Apigenin and Naringenin are compounds that have the potential to inhibit AGEs-RAGE signaling.
This was indicated by the stable complex formed from Apigenin-RAGE-AGEs and Naringenin-RAGE-AGEs.
REFERENCE
[1] Agustin, A.T.; A.Safitri.; F.Fatchiyah. An in Silico Approach Reveals the Potential Function of Cyanidin-3-o-glucoside of Red Rice in Inhibiting
the Advanced Glycation End Products (AGEs)-Receptor (RAGE) Signaling Pathway. Acta Inform Med. 28 (3): 170-179 (2020).
[2] Bongarzone, S.; V.Savickas.; F.Luzi.; A.D.Gee. Targeting the Receptor for Advanced Glycation Endproducts (RAGE): A Medicinal Chemistry
Perspective. Journal of Medicinal Chemistry. 60: 7213-7232 (2017).
[3] Kumar, M.; M.Tomar.; D.J.Bhuyan.; S.Punia.; S.Grasso, dkk. Tomato (Solanum lycopersicum L.) seed: A review on bioactives and biomedical
activities. Biomedicine & Pharmacotherapy. 142: 1-18 (2021).
[4] Perone, A.; A. Giovino.; J. Benny.; F.Martinelli. Advanced Glycation End Products (AGEs): Biochemistry, Signaling, Analytical Methods, and
Epigenetic Effects. Hindawi. 3: 1-18 (2020).
[5] Simbolon D.; A.Siregar.; R.A.Talib. Physiological Factors and Physical Activity Contribute to the Incidence of Type 2 Diabetes Mellitus in
Indonesia. National Public Health Journal. 15 (3): 120-127 (2020).
Oral Presentation
Effect of Black Cumin Seed Extract in Self Nanoemulsifying

Drug Delivery System (SNEDDS) on Histopathological Islet
Pancreas in Alloxan Diabetic Rattus norvegicus: An
Experimental Study
Syafira Laila Nurulita1*, Syaefudin Ali Akhmad2
1
Sleman, Special Region of Yogyakarta, Indonesia, Universitas Islam Indonesia, syafiralailanurulita@gmail.com
2
Sleman, Special Region of Yogyakarta, Indonesia, Universitas Islam Indonesia, aliakhmad@uii.ac.id
*Corresponding author: syafiralailanurulita@gmail.com
INTRODUCTION. Nigella sativa (Ns) has antidiabetic and protective effects on the pancreas 1. Ns is
hydrophobic so its oral bioavailability is low2. Self Nanoemulsifying Drug Delivery System (SNEDDS)
increases bioavailability thereby maximizing the therapeutic effect of Ns for the pancreas of DM patients3. The
aims of this study is to determine the effect of SNEDDS Ns on the histopathology of pancreatic islets in DM-
induced rats METHODS. The method used was a true-experimental post-test only control group design with
a sample of 35 Rattus norvegicus in 7 groups. K+F: without induction and intervention. K+P: alloxan and
glibenclamide. K-: alloxan and placebo. P1: alloxan and NonSNEDDS Ns 200mg/KgBB. P2: alloxan and
NonSNEDDS Ns 400mg/KgBB. P3: Alloxan and SNEDDS Ns 200mg/KgBB. P4: alloxan and SNEDDS Ns
400mg/KgBB. After the intervention of a month, histopathological observations of the pancreas were carried
out. RESULTS AND DISCUSSION. The mean number of pancreatic islet cells, respectively, was
P4(179.6±70.5); K+F(159.2±98.1); P3(132.6±20.6); P2(104.0±48.0); K-(80,80±55.5); K+P(78,56±34,4);
P1(71.0±28.7) (p=0.041). P3 and P4 are better than K+P and P3 and P4 are better than P1 (p<0.05). The mean
diameter of the islets of langerhans, respectively K+F(205.8±76.4); K+P(127.3±25.4); K-(120.9±38.8);
P1(131.9±13,4); P2(128.0±34.6); P3(135.4±15.9);P4(180.7±50,0) (p=0.123). CONCLUSION. This study
shows that there was an effect of SNEDDS black cumin extract at a dose of 200mg/KgBB on the number of
cells in the pancreatic islets in Rattus norvegicus after DM induction with Alloxan.
REFERENCE
[1] Abdelmeguid NE, Fakhoury R, Kamal SM, Al Wafai RJ. Effects of Nigella sativa and thymoquinone on biochemical and subcellular changes in
pancreatic β-cells of streptozotocin-induced diabetic rats. J Diabetes. 2010;2(4):256–66.
[2] Procopet B, Berzigotti A. Diagnosis of cirrhosis & portal hypertension: Imaging, non-invasive markers of fibrosis & liver biopsy. Gastroenterol
Rep. 2017;5(2):79–89.
[3] Kalam MA, Raish M, Ahmed A, Alkharfy KM, Mohsin K, Alshamsan A, et al. Oral bioavailability enhancement and hepatoprotective effects of
thymoquinone by self-nanoemulsifying drug delivery system. Mater Sci Eng C [Internet]. 2017;76:319–29. Available from:
http://dx.doi.org/10.1016/j.msec.2017.03.088
Oral Presentation
Effect of Black Cumin Extract in Self Nanoemulsifying Drug

Delivery System Preparation on Histopathological
Appearance of Rattus Norvegicus Myocardium Post Alloxan
Diabetes Mellitus Induction
Talenta Nugroho Suryanto Mahardhika1*, Syaefudin Ali Akhmad2

1
2
*Corresponding author: mahardhika.talenta@gmail.com
INTRODUCTION. People with diabetes mellitus have a higher risk of heart disease.1 Treatment for diabetes
mellitus is carried out for life using chemical drugs that can cause various side effects.2,3 Nigella sativa has the
active substance thymoquinone which has antioxidant and antidiabetic effect but has low bioavailability.4,5 Self
Nanoemulsifying Drug Delivery System (SNEDDS) is able to increase the bioavailability of substances by
increasing the solubility of these substances.6 The aim of the study is to determine the effect of Nigella sativa
extract in SNEDDS preparation on histopathological appearance of Rattus norvegicus myocardium after
diabetes mellitus induction with alloxan. METHODS. Researchers use the “true-experimental” and a posttest
only control group design by utilizing 35 male rats induced with diabetes by administering alloxan (150
mg/KgBW) intraperitoneally and intervened for 4 weeks. Rats categorized into seven groups: Physiological
positive control (K+F), pathological positive control (K+P) administered with glibenclamide, negative control
(K-) administered with placebo, P1 administered with black cumin extract 200 mg/KgBW/day, P2
administered with black cumin extract 400 mg/KgBW/day, P3 administered with black cumin extract in
SNEDDS preparation 200 mg/KgBW/day, and P4 administered with black cumin extract in SNEDDS
preparation 400 mg/KgBW/day. Subsequently, the result of histopathological examination of the heart is
analyzed with SPSS 24 statistical software. RESULTS AND DISCUSSION. The result of the myocardial
degeneration measurement are obtained mean (µm2) K+F (6748,1), K+P (0), K- (6026,6), P1 (7378,6), P2
(6980), P3 (5344,7), P4 (0). There were no significant differences between the group (p=0,77).
CONCLUSION. Black cumin extract in SNEDDS preparation does not significantly affect the
histopathological changes of rats heart after alloxan induction of diabetes mellitus.
REFERENCE
[1] Bamosa, A. et al. Nigella sativa: A potential natural protective agent against cardiac dysfunction in patients with type 2 diabetes
mellitus, Journal of Family and Community Medicine. (2015).
[2] Kurniawaty, E. and Lestari, E. E. Uji Efektivitas Daun Belimbing Wuluh (Averrhoa bilimbi L.) sebagai Pengobatan Diabetes
Melitus. Majority. (2016).
[3] Soelistijo, S. A. et al. Pedoman pengelolaan dan pencegahan diabetes melitus tipe 2 dewasa di Indonesia 2019. Perkumpulan
Endokrinologi Indonesia. (2019).
[4] Atta, M. S. et al. Diabetic cardiomyopathy: Pathophysiology, theories and evidence to date. World Journal of Diabetes. (2018).
[5] Elmowafy, M. et al. Enhancement of Bioavailability and Pharmacodynamic Effect of Thymoquinone Via Nanostructured Lipid
Carrier (NLC) Formulation, AAPS PharmSciTech. (2016).
[6] Kazi, M. et al. Evaluation of self-nanoemulsifying drug delivery systems (SNEDDS) for poorly water-soluble talinolol:
Preparation, in vitro and in vivo assessment. Frontiers in Pharmacology. (2019).
Oral Presentation
Pathophysiological Analysis of kidney and comparison of male

and female SDT fatty rats, an obese model of type 2 diabetes
mellitus, fed a high sucrose and high fat diet
Kana Watanabe1*, Katsuhiro Miyajima1,2, Kouhei Mandai1, Keita Sekiguti1, Noriko
Kemuriyama1,2, Tomohiko Sasase5, Toshihisa Watanabe3, Hideki Ito3, Masami Shinohara4
and Takeshi Ohta5.
1
Dept. Nutri. Sci. Food Safety, Fac. Applied Bioscience, Graduate School of Tokyo Univ. of Agricul.
2
Dept.Nutri. Sci. Food Safety, Fac. Applied Bioscience, Undergraduate School of Tokyo Univ. of Agricul.
3
CLEA Japan, Inc. Fuji Breeding Facility
4
CLEA Japan Inc. Business Promotion Dept.
5Laboratory of Animal Physiology and Functional Anatomy, Graduate School of Agriculture, Kyoto University.
*Corresponding author: (wkana0327@outlook.jp)
Background and Aim. Type 2 diabetic model SDT fatty rats (SDTf rats) develop severe diabetes with obesity
and male rats show severer phenotype compared to female rats. However, the effects of food on their kidney
have not been reported. In this study, we investigated the effect of high-sucrose/high-fat diet (Quick fat; QF)
on kidney of SDTf rats, including sex differences. Materials and Methods. Four-week-old male and female
SDTf rats were fed the standard diets (CE-2) or QF (CLEA Japan Inc.). During the feeding period, body
weights, food intake and blood glucose levels were measured. At 27 weeks of age in male and 38 weeks of
age in female, animals were necropsied, and organ weights were measured, and blood and kidneys were
sampled. Blood biochemistry, histopathological observation in kidneys and gene expression analysis were
conducted using the collected samples. Result. During the feeding period, some SDTf rats fed QF (both sex)
died. A postmortem examination revealed that the cause was worsening renal dysfunction. In male SDTf rats,
histopathological findings were as follows; enlargement, adhesion, mesangial hyperplasia and fibrosis in
glomerulus and formation of urinary cast, dilation, regeneration, Armanni-Ebstein lesion, and inflammatory
cell infiltration in tubules. Each lesion tended to worsen with QF feeding. Similar histopathological changes
were found in female SDTf rats, however, female showed severer mesangial hyperplasia, macrophage
infiltration and fibrosis in glomerulus and milder lesions in tubules when compared to male. Conclusion. From
these results, QF feeding exacerbates legal lesion in SDTf rats in both sexes and it might be as a new diabetic
kidney disease (DKD) model for tubules in male and for glomerulus in female.
QF Inflammation
Fatty
metamorphosis
DM
REFERENCE
[1]CLEA JAPAN Inc.“SDT fatty/Jcl”. https://www.clea-japan.com/products/diabetes/item_a0150
[2] ]CLEA JAPAN Inc.“Quick Fat”. https://www.clea-japan.com/products/general_diet/item_d0070
Oral Presentation
Investigation of the usefulness of hL-FABP tg mice as

biomarkers for NAFLD/NASH.
Marika TOHMA1*, Keiichi Ohata2,3, Ayaka HORIUCHI2 Teppei Uechi1, Noriko

KEMURIYAMA1,3, and Katsuhiro Miyajima1,3
1
Department of Nutritional Science and Food Safety, Graduate School of Applied Biosciences,
Tokyo University of Agriculture,
2
Department of Nutritional Science and Food Safety, Faculty of Applied Biosciences, Tokyo
University of Agriculture,
3
L-FABP Business Department, CMIC Holdings Co., Ltd, Tokyo, Japan,
*Corresponding author: (email),
INTRODUCTION. The mechanism leading to the onset and progression of nonalcoholic fatty liver disease
(NAFLD)/nonalcoholic steatohepatitis (NASH) remains unclear, and no curative treatment has been
established. Liver-type fatty acid binding protein (L-FABP) is localised in the liver and kidney and plays a
central role in intracellular lipid transport. It is also increased by ischemia and oxidative stress. L-FABP has
already been reported to be useful as a biomarker for NASH in humans, but its knowledge is still limited. We
used choline-deficient methionine-reduced amino acid diet (CDAA). Choline and methionine are materials for
VLDL and release triglycerides out of the liver. By reducing these, triglycerides are not released into the blood
and accumulate in the liver. By these mechanisms, CDAA diet feeding induces oxidative stress, fatty liver and
fibrosis. We aimed to investigate the potential of blood L-FABP as a biomarker in the early stages of liver
disease using an animal model of CDAA-induced NAFLD/NASH. METHODS. Sixteen-week-old male hL-
FABP tg mice were fed with CDAA or HFD for 1, 3, 7, or 14 days, respectively. After each feeding period,
the mice were dissected for histopathological observation of the liver, blood biochemical examination, and
measurement of hL-FABP concentration by ELISA. A control group was fed a standard diet (SD) for 14 days.
RESULTS AND DISCUSSION. Blood hL-FABP levels were increase over time with CDAA.
Histopathological observation showed that on day 1, diffuse fatty changes of hepatocyte in CDAA or focal
change in marginal zone in HFD. The area of fatty change became wider over time on 3, 7 or 14 -days of
feeding. CONCLUSION. The concentration of hL-FABP was detected at an earlier stage than the increase in
blood AST and ALT activities, indicating that L-FABP should be useful as a biomarker in the early stages of
NAFLD.
REFERENCE
[1] Tokunori Yamamoto, JASN November 2007, 18 (11) 2894-2902.
[2] Masahiko Matsumoto, Int. J. Exp. Path. (2013), 94, 93–103
[3] Akiko Eguchi , Pharmaceutical Research volume 38, pages89–95 (2021).
Oral Presentation
Anti-angiogenic effect of non-contact electro capacitive cancer

therapy on DMBA-induced rat breast tumor model
Endah Sri Palupi1, Bambang Retnoaji2, Pudji Astuti3, Firman Alamsyah4, Rarastoeti
Pratiwi5*
1
Postgraduate Program, Faculty of Biology, Universitas Gadjah Mada
2
Animal Structure and Development Laboratory, Faculty of Biology, Universitas Gadjah Mada
3
Department of Physiology, Faculty of Veterinary, Universitas Gadjah Mada
4
Center for Medical Physics and Cancer Research, Ctech Labs Edwar Technology, Tangerang
5
Biochemistry Laboratory, Faculty of Biology, Universitas Gadjah Mada
*Email corresponding: rarastp@ugm.ac.id
Electro Capacitive Cancer Therapy (ECCT) is a non-contact anti-tumor device with an anti-proliferative and
pro-apoptotic capability in inhibiting tumor growth, particularly breast tumor, but its effect on angiogenesis is
unclear. Angiogenesis contributes to tumor growth and metastasis. VEGFA is a primary growth factor and
HIF1α is its transcription factor during angiogenesis. Both mRNA expressions induce angiogenesis, while
their blocking may inhibit tumor growth. Twenty-four rats were divided into 4 groups. Two groups of non-
DMBA-induced rats, while the other 2 groups were DMBA-induced rats, each with and without ECCT therapy.
ECCT exposure was 150 kHz and 18 Vpp of the alternating current (AC) source. Mammary gland and breast
tumor tissue were collected and preserved. Gene expression was measured using qPCR. The following primers
were F-5’-GCCTCAGGACATGGCACTAT-3’ and R-5’-GGAGGAGGAGGAGCCATTAC-3’ for VEGFA,
while F-5’-TGCTCATCAGTTGCCACTTC-3’ and R-5’-CCATCCAGGGCTTTCAGATA-3’ for HIF1α.
Relative gene expressions were analyzed using the Livak method with GAPDH normalization. The data were
statistically analyzed using ANOVA and LSD test. The results show that ECCT exposure has no impact on
VEGFA and HIF1α expression of normal breast tissue. HIF1α mRNA expression has been downregulated
insignificantly by ECCT exposure, but the significant downregulation resulted in VEGFA mRNA expression
after ECCT therapy. It suggests the possibility of other pathways, otherwise HIF1α-dependent pathway. The
HIF1α-independent pathway may regulate VEGFA expression or both pathways co-operate. Thus, further
research will be necessary.
Keywords: angiogenesis, breast tumor, ECCT exposure, HIF1α, VEGFA
REFERENCE
1. Giladi M, Schneiderman RS, Voloshin T, Porat Y, Munster M, Blat R, et al. Mitotic Spindle Disruption by Alternating Electric
Fields Leads to Improper Chromosome Segregation and Mitotic Catastrophe in Cancer Cells. Sci Rep. 2015 Dec 11;5.
2. Mursilatun. PENGARUH MEDAN LISTRIK TERHADAP PERTUMBUHAN SEL KANKER. Fakultas Matematika dan Ilmu
Pengetahuan Alam, Universitas Indonesia. Depok; 2010.
3. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global Cancer Statistics 2020: GLOBOCAN
Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021 May;71(3):209–49.
4. Alamsyah F, Niswah Ajrina I, Nur F, Dewi A, Iskandriati D, Prabandari SA, et al. Antiproliferative Effect of Electric Fields on
Breast Tumor Cells In Vitro and In Vivo. Indones J Cancer Chemoprevention. 2015;
5. Alamsyah F, Fadhlurrahman AG, Pello JI, Firdausi N, Evi S, Karima FN, et al. PO-111 Non-contact electric fields inhibit the
growth of breast cancer cells in animal models and induce local immune reaction. ESMO Open. 2018 Jun;3:A269.
6. Mujib SA, Alamsyah F, Taruno WP. Cell Death and Induced p53 Expression in Oral Cancer, HeLa, and Bone Marrow
Mesenchyme Cells under the Exposure to Noncontact Electric Fields. Integr Med Int. 2017 Dec 21;4(3–4):161–70.
7. Chusnu RA, Sahudi, Muhtarum Y, Urip M, Dwi SA, Khairina, et al. The Electro-Capacitive Cancer Therapy (ECCT) as
Adjuvant Therapy for Advanced Stage Nasopharyngeal Carcinoma. 18th ASEAN ORL-HNS CONGRESS. 2019.
8. Alamsyah F, Pratiwi R, Firdausi N, Irene Mesak Pello J, Evi Dwi Nugraheni S, Ghitha Fadhlurrahman A, et al. Cytotoxic T
cells response with decreased CD4/CD8 ratio during mammary tumors inhibition in rats induced by non-contact electric fields.
F1000Research. 2021 Jan 19;10:35.
9. Pratiwi R, Antara NY, Fadliansyah LG, Ardiansyah SA, Nurhidayat L, Sholikhah EN, et al. CCL2 and IL18 expressions may
associate with the anti-proliferative effect of noncontact electro capacitive cancer therapy in vivo. F1000Research. 2019;8.
10. Vogelstein B, Kinzler KW. Cancer genes and the pathways they control. Nat Med. 2004;10(8):789–99.
11. Mittal V. Epithelial Mesenchymal Transition in Tumor Metastasis. Annu Rev Pathol Mech Dis. 2018;13:395–412.
12. Hanahan D, Weinberg RA. The Hallmarks of Cancer Review evolve progressively from normalcy via a series of pre. Vol. 100,
Cell. 2000.
13. Harmey JH. VEGF and Cancer. VEGF and Cancer. 2004.
14. Roskoski R. Vascular endothelial growth factor (VEGF) signaling in tumor progression. Vol. 62, Critical Reviews in
Oncology/Hematology. 2007. p. 179–213.
15. Curtis KK, Wong WW, Ross HJ. Past approaches and future directions for targeting tumor hypoxia in squamous cell
carcinomas of the head and neck. Crit Rev Oncol Hematol [Internet]. 2016;103:86–98. Available from:
http://dx.doi.org/10.1016/j.critrevonc.2016.05.005
16. LaGory EL, Giaccia AJ. The ever-expanding role of HIF in tumour and stromal biology. Nat Cell Biol. 2016;18(4):356–65.
17. Kim EH, Song HS, Yoo SH, Yoon M. Tumor treating fields inhibit glioblastoma cell migration, invasion and angiogenesis.
Oncotarget. 2016;7(40):65125–36.
18. Wu L, Wu Y, Xiong Z, Yao C, Zeng M, Zhang R, et al. Effects and possible mechanism of a picosecond pulsed electric field on
angiogenesis in cervical cancer in vitro. Oncol Lett. 2019 Feb 1;17(2):1517–22.
Oral Presentation
Enzymatic Synthesis and Cytotoxicity Activity of Phenolic

Acid Glucosyl Ester in Cholangiocarcinoma Cells
Eko Suyanto1,2,3,4, Chutima Talabnin1, Jaggaiah Naidu Gorantla2, and James R. Ketudat-
Cairns1,2*
1
Biochemistry and Biochemical Technology Program, School of Chemistry, Institute of Sciences, Suranaree University
of Technology, Muang, Nakhon Ratchasima, Thailand
2
Center for Biomolecular Structure, Function and Application, Suranaree University of Technology, Muang, Nakhon
Ratchasima, Thailand
3
Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, East Java,
Indonesia
4
Research Center of Smart Molecule of Natural Genetics Resource, Brawijaya University, Malang, East Java, Indonesia
*Corresponding author: cairns@sut.ac.th
Rice Os9BGlu31 (EC 3.2.1.21) is an enzyme in glycoside hydrolase family 1 (GH1), a family that
mostly catalyzes hydrolysis reactions. Os9BGlu31, however, mainly has transglycosylation activity that can
transfer a glucosyl moiety to new aglycon moieties to form glucoconjugates through a retaining mechanism
[1]–[3]. This reaction may improve stability, solubility, bioactivity, bioavailability, and physicochemical and
physiological properties of the compounds. In this study, we investigated the ability of rice Os9BGlu31
transglucosidase for glycosylation of phenolic acids to synthesize glucosyl esters. Os9BGlu31 and its mutants
were expressed in Escherichia coli strain Origami B(DE3), then purified by an immobilized metal affinity
chromatography (IMAC). The glucoconjugate products were obtained by transglycosylation reactions, then
detected by thin layer chromatography (TLC), and measured by ultra-high performance liquid chromatography
(UHPLC) and their structures were verified by Nuclear Magnetic Resonance (NMR) spectroscopy.
Os9BGlu31 transglucosidase and its mutants transferred a glucosyl moiety from p-nitrophenol β-D-
glucopyranoside as glucose donor to phenolic acids. Os9BGlu31 mutants had higher activity than wildtype on
phenolic acids to produce their glucosides and glucosyl esters. Meanwhile, Os9BGlu31 wildtype tended to
produce a single product of phenolic acid glucosyl ester. One of the most interesting products was β-
glucogallin, which had higher antioxidant activity (IC50 value = 3.58 ± 0.10 µg/ml) than other phenolic acid
glucosyl esters. β-Glucogallin has cytotoxicity in cholangiocarcinoma cells (KKU-213A, KKU-100 and KKU-
055) and its highest cytotoxicity on KKU-055, with an IC50 value of 4.89 ± 0.14 µM on KKU-055. In contrast,
β-glucogallin has no cytotoxicity activity on a normal fibroblast cell line (IMR-90). Rice Os9BGlu31
transglucosidase is promising enzyme for production of β-glucogallin, which may act as an anti-cancer agent
to inhibit the cell proliferation of cholangiocarcinoma cells.
Keywords: Os9BGlu31, transglycosylation, β-glucogallin, cytotoxicity, anti-cancer.
This research was supported by International Research Network grant IRN62W0004 from the Thailand
Research Fund.
REFERENCE
[1] J. R. K. Cairns and A. Esen, “β-Glucosidases,” Cell. Mol. Life Sci., vol. 67, no. 20, pp. 3389–3405, 2010.
[2] S. Luang et al., “Rice Os9BGlu31 is a transglucosidase with the capacity to equilibrate phenylpropanoid, flavonoid, and
phytohormone glycoconjugates,” J. Biol. Chem., vol. 288, no. 14, pp. 10111–10123, 2013.
[3] J. Komvongsa et al., “Active site cleft mutants of Os9BGlu31 transglucosidase modify acceptor substrate specificity and allow
production of multiple kaempferol glycosides,” Biochim. Biophys. Acta - Gen. Subj., vol. 1850, no. 7, pp. 1405–1414, 2015.
Oral Presentation
BRAZILIN AND 3 – O- METHYL BRAZILIN REVEALED

THERAPEUTIC AGENT FOR ECZEMA: MOLECULAR
DOCKING APPROACH
Dewi Ratih Tirto Sari1,2,*, Venny Dyah Ningsih1, Fatinatul Lailiyah1
1
Pharmacy Department, Faculty of Medical Science, Universitas Ibrahimy, Situbondo
2
Research Center of Smonagenes, Brawijaya University, Malang, Indonesia
*Corresponding author: dewiratihtirtosari@ibrahimy.ac.id
INTRODUCTION. Caesalpinia heartwood is a local wood plant in Indonesia, containing several bioactive
metabolites and was used for natural cloth dye, herbal medicines, and others [1, 2]. Caesalpinia sappan
heartwood extract was reportedly contains 29 compounds that perform antidiabetic, antioxidant, antibacterial,
and anti-inflammatory activities [2, 3]. One of inflammatory diseases is eczema, that expressed on the skin.
This study identified the potential activity of the brazilin and 3-O-methyl brazilin as therapeutic agents for
eczema through molecular docking approach. METHODS. The JAK-STAT protein pathway for eczema,
included JAK3, RORC, PRKCQ, and IL-6, were used as an eczema protein target therapy. Those 3D structures
were downloaded from Protein Data Bank. Brazilin and 3 – O – methyl brazilin were a bioactive ingredients
of Caesalpinia sappan heartwood were retrieved from the PubChem NCBI database with the CID 73384 and
CID 13846641. Those proteins and compounds were docked by Molegro virtual Docker 5 in the active sites
of proteins, then were visualized by using Discovery studio and PyMol version 2.2 [4]. RESULTS AND
DISCUSSION. Molecular docking showed the interaction both brazilin and 3 – O- Methyl brazilin at the
inhibitor sites of JAK3 protein. furthermore, 3D complex structure also revealed binding activity of Brazilin
and 3 – O – methyl brazilin with RORC, PRKCQ, and IL6 at the active sites. Two-dimensional complex
structures performed hydrogen bonds, hydrophobic interactions, and van der Waals, which were contributed
to the low binding energy of those ligand – proteins complex. CONCLUSION. We summarized that brazilin
and 3 -3 – O methyl brazilin inhibited the JAKSTAT pathway revealing low erythema and might have potential
as eczema antagonist. Further in vivo experiment was required for supporting in silico study.
REFERENCE
[1] Dapson RW, Bain CL. Biotechnic and Histochemistry 2015; 90: 401–423.
[2] Mueller M, Weinmann D, Toegel S, et al. Food and Function 2016; 7: 1671–1679.
[3] Warinhomhaun S, Sritularak B, Charnvanich D. Thai Journal of Pharmaceutical Sciences 2018; 42: 208–213.
[4] Bitencourt-Ferreira G, de Azevedo WFJ. Methods in molecular biology (Clifton, NJ) 2019; 2053: 149–167.
Oral Presentation
Anti-inflammatory effects of Channa micropeltes extract

through NF-κβ and TNF-α in diabetic rat
Amy Nindia Carabellya, Juliyatin Putri Utamib*, Didit Aspriyantoc, Milka Widya Sarid, Erika
Norfitriyahe
a
Department of Oral Pathology and Maxillofacial, Faculty of Dentistry, University of Lambung Mangkurat,
Banjarmasin, Indonesia
b
Department of Biomedicine Faculty of Dentistry, University of Lambung Mangkurat, Banjarmasin, Indonesia
c
Department of Oral biology Faculty of Dentistry, University of Lambung Mangkurat, Banjarmasin, Indonesia
d
Faculty of Dentistry, University of Lambung Mangkurat, Banjarmasin, Indonesia
e
Department of Pediatric dentistry Faculty of Dentistry, University of Lambung Mangkurat, Banjarmasin, Indonesia
E-mail:juliyatin.utami@ulm.ac.id
Inhibition of tissue repair is a symptom of prolonged diabetic inflammation that occurs in wounds
resulting in delayed healing. Inflammation can be controlled by giving food supplements that have
anti-inflammatory properties such as Channa micropeltes (CM). CM has high protein content
including albumin, omega 3 and omega 6 fatty acids, vitamin C, and zinc. Therefore, this study aimed
to analyze the effect of CM extract on the levels of molecular markers Nf-κB and TNF-α in the wound
healing process of normal and diabetic rats. This study was a true experimental design with a post-
test-only control group design. The samples were the diabetic model group given CM extract at 16
mL/kgBB dose and negative control group for 14 days. DM-CM group showed decreasing level of
NFkB. TNF- α was detected with lowest level in N-CM group. The levels of TNF- α declined close
to normal levels in the DM-CM group. Hence, the conclusion was the CM at a dose of 16 mL/kg BW
for 14 days in the wound healing process of Wistar rats can reduce Nf-κB and TNF-α in groups of
normal and diabetic rats.
Oral Presentation
Computational and in vitro experimental evidence to the

anti-migration activity of Cucurbitacin-B
He Huifu1,2, Huayue Zhang1,2, Durai Sundar3, Renu Wadhwa1,2 and Sunil Kaul 1,2*
Graduate School of Science and technology, University of Tsukuba, Ibaraki 305-8575, Japan
1
AIST-INDIA DAILAB, National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba –
2
305-8565, Japan
3
DAILAB, Department of Biochemical Engineering & Biotechnology, Indian Institute of Technology (IIT)-Delhi, New
Delhi 110-016, India
*Corresponding author: Sunil Kaul Email: s-kaul@aist.go.jp
INTRODUCTION. Cancer metastasis, a highly complex transformation of cancer cells, is mostly treated with
synthetic chemotherapeutic molecules that are known to cause severe adverse effects and are very expensive.
Natural compounds, on the other hand, are considered safe, easily available and economic. Cucurbitacin-B
(Cuc-B; 19-(10→9β)-abeo-10-lanost-5-ene triterpene) is a steroid with peculiar bitter taste commonly found
in the plants of cucurbitaceae and other families such as brassicaceae, salicaceae, chrysobalanaceae,
tricholomalaceae and begoniaceae. Withanone (Wi-N; 6,7-epoxy-5,17-dihydroxy-1-oxowitha-2,24-dienolide)
is a c6, c7 epoxy steroidal lactone, one of the major withanolides found in withania somnifera. While both Cuc-
B and Wi-N are know to possess anticancer potential, their combination CucWi-N was shown to offer better
anti-cancer activity [1]. METHODS. Effect of Cuc-B, Wi-N and their combination on proliferation and
migration characteristics of cancer cells was determined by in vitro assays. Protein expression was investigated
by Western blotting and immunocytostaining with specific antibodies. Docking potential of each of the
compounds to proteins involved in cancer cell migration was dtermined by Molecular docking tools.
RESULTS AND DISCUSSION. Cancer cells treated with Cuc-B and Wi-N showed growth arrest/apoptosis.
The combination, CucWi-N, caused induction of cellular senescence and decrease in migration and was
supported by molecular analyses of biomarkers involved in these phenotypes. Molecular dynamics analyses
revealed that Cuc-B and Wi-N have ability to disrupt mortalin-p53 interaction yielding nuclear translocation
and activation of wild type p53 function. Furthermore, interaction of Cuc-B with two proteins (Mortalin and
HDM2) that are enriched in cancer cells, negatively regulate p53 and promote cancer cell migration showed
that it has capability to interact with HDM2 and was similar to its known inhibitor Y30. Furthermore,
downregulation of mortalin, MMP-3 and hnRNP-K proteins that are critically involved in cell migration was
observed suggesting that Cuc-B is a potential natural drug that warrants further mechanistic and clinical studies
for its use in management of metastatic cancers. CONCLUSION. Cuc-B is a potential natural drug that
warrants further mechanistic and clinical studies for its use in management of metastatic cancers.
REFERENCE
[1] Sukant.G, et al. J Gerontol A Biol Sci Med Sci, 2020, Vol. 75, No. 6, 1031–1041
Oral Presentation
In vitro cell culture-based evaluation of antistress potentials of

fucoxanthin
Huayue Zhang1, Mallika Khurana2, Motomichi Doi3, Sunil C. Kaul4 and Renu Wadhwa5*
1
Graduate School of Life & Environmental Sciences, University of Tsukuba, Ibaraki 305-8575, Japan;
Japan. Email: zhang-huayue@aist.go.jp
2
Japan. Email: mallikakhurana98@gmail.com
3
Molecular Neurobiology Group, Biomedical Research Institute, National Institute of Advanced Industrial Science &
Technology (AIST), Tsukuba 305-8565, Japan. Email: doi-m@aist.go.jp
4
Japan. Email: s-kaul@aist.go.jp
5
Japan. Email: renu-wadhwa@aist.go.jp
*Corresponding author: renu-wadhwa@aist.go.jp
INTRODUCTION. A variety of environmental stresses have been shown to play a key role in determining
the Quality of life (QOL) especially towards later years of human lifespan when there is an accumulated
increase in intrinsic stresses and decrease in efficiency of defence/damage-repair processes. Stress
management with natural compounds is a priority research field as it is expected to decrease the disease burden
and hence good for the health care systems. In this study, we examined the antistress potential of fucoxanthin,
a popular carotenoid found in marine organisms and known for its antioxidant and cytoprotective capacities
[1]. METHODS. An in vitro replicative senescence-based physiologically relevant stress model was
developed by serial passaging of human normal lung fibroblast (TIG-3) by 1:8 split ratio to achieve three
population doublings (PD) per passage. Cells were frozen at each passage until they reached senescence (PD
68) as confirmed by SA-β-Gal staining. Young, mid- and old-age fibroblasts representing the progressively
increasing states of intrinsic stress were treated with extrinsic stresses followed by recovery either in the normal
or fucoxanthin supplemented medium. Phenotypic, biochemical and imaging based analyses were undertaken
using various stress models. RESULTS AND DISCUSSION. Phenotypic and molecular analyses of control,
stressed (with or without fucoxanthin) cells showed that fucoxanthin offered protection against the stress
induced (i) apoptosis, (ii) ROS accumulation coupled with mitochondrial depolarization and (iii) DNA double
strand breaks. As expected, the replicative senescent model revealed accumulation of oxidative stress and
DNA damage in the old vs young fibroblasts. Of note, we found that fucoxanthin offered protection against
the oxidative stress, UV and metal induced cytotoxicity both in the young and old fibroblasts. Furthermore, in
a unique genetic model of amyloid beta aggregation (an established causative factor of Alzheimer’s disease),
fucoxanthin was seen to cause deaggregation of beta-amyloid yielding protection against beta-amyloid induced
cytotoxicity. CONCLUSION. Taken together, these results demonstrated antistress potentials of fucoxanthin
that may be useful for management of stress and old-age related pathologies.
REFERENCE
[1] Afzal, S., et al. Mar. Drugs 17, 189 (2019).
Oral Presentation
Phytochemical Screening and In-vitro Antioxidant Activity of

leaf extract of Clinacanthus nutans
Nurlaili Susanti1,2, Arifa Mustika3*, Djunaidi Khotib4
1
Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Indonesia,
nurlaili.susanti-2020@fk.unair.ac.id
2
Faculty of Medicine and Health Science, UIN Maulana Malik Ibrahim, Indonesia
3
Department of Anatomy, Histology, and Pharmacology, Faculty of Medicine, Universitas Airlangga, Surabaya,
Indonesia, arifa-m@fk.unair.ac.id
4
Department of Pharmacy Practice, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia,
junaidi-k@ff.unair.ac.id
*Corresponding author: arifa-m@fk.unair.ac.id
Abstract.
INTRODUCTION. Antioxidant properties of medicinal plants have been demonstrated to play a protective
role against oxidative stress-related diseases in the body. Phenolic compounds, including phenols and
flavonoids, contribute to plants’ antioxidant capacity. Clinacanthus nutans, native to Asia, including Malaysia,
Thailand, and Indonesia, are used for treating diabetes and many other diseases, but the exploration of this
plant in Indonesia is still limited. The purpose of this research was to explore the phytochemical constituents
and antioxidant activities of ethanol and aqueous extracts. METHODS. 70% ethanol and aqueous were used
to extract dried leaf powder. A qualitative phytochemical screening was performed on each extract. Total
phenolic and flavonoid content was calculated using Folin-Ciocalteu method and colorimetric assay with
aluminum chloride. Antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical
scavenging method. RESULTS. Qualitative phytochemical test showed that extracts contain phenolic,
flavonoid, tannin, and saponin. Total phenolic content was found to be 4,14±1,9% mgGAE/g (ethanol extract)
and 3,89±3,2% mgGAE/g (aqueous extract). Total flavonoid content was found to be 0,19±1,0% mgQE/g
(ethanol extract) and 0,03±0,5% mgQE/g (aqueous extract). Both extracts showed high antioxidant activity
(DPPH IC50 30.57 µg/ml for ethanol extract and 37.24 µg/ml for aqueous extract). CONCLUSION. C. nutans
is a good source of natural antioxidants. The ethanol extract had a higher total phenolic and flavonoid content
as well as more powerful antioxidants than the aqueous extract.
Keywords: Phytochemical screening, total phenolic content, total flavonoid content, antioxidant activity,
Clinacanthus nutans.
Oral Presentation
Lactate dehydrogenase (LDH) in the vagina of ratus

norvegicus wistar after exposure to candida albicans at 12, 24
and 48 hours
Nurdiana1, Sri Winarsih2, Agustina Tri Endharti3, Sri Handayani4,5*
1
Departement of Microbiology Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia,
2
Departementof Pharmacy, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia,
3
Departementof Parasitology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia,
4
Doctoral Program of Medical Science, Faculty of Medicine, Brawijaya University, Malang,
Indonesia, 5Departement of Midwifery of Aisyiyah Surakarta University,
*Corresponding author: nurdianafarmako.fk@ub.ac.id
Candida albicans is a fungal pathogen that is one of the causes of candidiasis. The cytotoxic potential
of candida albican infection is characterized by changes in lactate dehydrogenase levels. This study
aims to determine the effect of holothurin and caspofungin on the degree of infection seen from levels
of lactate dehydrogenase (LDH). The sample in this study was the experimental animal Rattus
norvegicus wistar, which was divided into 6 treatment groups. Negative control (K1), positive
control/c.albicans (P1), holothurin (P2), caspofungin (P3), c. albicans + holothurin (P4), and c.
albicans + caspofungin (P5). Based on the results obtained the average and standard deviation of
lactate dehydrogenase levels (LDH). K1 (336 ± 16), P1 (338 ± 31), P2 (331 ± 29), P3 (377 ± 45), P4
(350 ± 5), P5 (386 ± 33). LDH did not change significantly in both the negative and positive groups
for candida albican, as well as after administration of antifungal therapy in the form of holothurin and
caspofungin at 12, 24 and 48 hours.
Kata kunci: LDH, candida albican, antifungal
Oral Presentation
Pharmaceutical Application of Microcapsules from The

Combination of Ruellia tuberosa L. and Cosmos caudatus K.
Extracts
Choirin Annisa1*, Sasangka Prasetyawan1, and Anna Safitri1,2
1
Chemistry Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, 65145,
Indonesia (email: choirinannisa@student.ub.ac.id)
2
Research Center for Smart Molecules of Natural Genetic Resources (SMONAGENES), Brawijaya University,
Malang, 65145, Indonesia
*Corresponding author: a.safitri@ub.ac.id
INTRODUCTION. The combination of R. tuberosa L. and C. caudatus K. extracts is expected to have

potential as a candidate for an antidiabetic agent. Each of these plants has biological activity derived from
bioactive compounds, such as cirsimarin and quercetin, which have low bioavailability. The bioavailability of
the bioactive compounds can be increased through microencapsulation. Microencapsulation is an extract
coating method used as a drug delivery system. Microencapsulation was carried out with chitosan-Na-TPP
(sodium tripolyphosphate) coating material. METHODS. Microencapsulation was made by varying the pH of
chitosan (3, 4, 5, and 6), the concentration of Na-TPP (0.15; 0.20; 0.25; and 0.30% (w/v)), and stirring time
(30, 60, 90, and 120 minutes). The optimum conditions for microcapsules were selected based on the highest
percentage of encapsulation efficiency (% EE), calculated from the total flavonoid content. The optimum
condition of microcapsules was then characterized using FTIR, SEM, and PSA instruments. Followed by the
antidiabetic activity test using the alpha-amylase enzyme inhibition method and the antioxidant activity test
using the DPPH method. Finally, the release test was carried out using HCl pH 1.2 and phosphate buffer pH
7.4 medium. RESULTS AND DISCUSSION. The optimum condition of microcapsules was obtained at pH
4, Na-TPP concentration of 0.15% (w/v), and stirring time of 60 minutes with 96.25 ± 0.11 % encapsulation
efficiency. The FTIR spectrum showed that the P=O functional group of Na-TPP was 1213.71 cm-1 and the C-
N of chitosan was 1155.23 cm-1. Characterization by SEM and PSA showed the microcapsules were spherical
and had an average diameter of 132.08 µm. The alpha-amylase inhibition test and antioxidant activity results
were expressed as IC50 values of 223.64 ± 0.81 µg/mL and 104.05 ± 0.88 µg/mL, respectively. The bioactive
compounds released at pH 1.2 and 7.4 within 120 minutes were 5.99% and 58.96%, respectively.
CONCLUSION. This study shows that microencapsulation is an appropriate strategy to enhance the
pharmaceutical application of extract combinations.
REFERENCE
[1] Annisa, C., Prasetyawan, S., and Safitri, A., Co-microencapsulation of Ruellia tuberosa L. and Cosmos caudatus K. Extracts for Pharmaceutical
Applications, Makara Journal of Science. 26, 2, 96-106 (2022).
Oral Presentation
Effect of Recombinant Protein Nano-vaccine C. vulgaris on

Interferon-Gamma Gene Response and Growth in Grouper
Uun Yanuhar1*, Abd. Rahem Faqih2, Septi Anitasari1, Nur Sakinah Junirahma3 and Nico
Rahman Caesar4
1
Departement of Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, University of Brawijaya,
Malang, Jl. Veteran, Malang – East Java, Indonesia, 65145
2
Departement of Aquaculture, Faculty of Fisheries and Marine Sciences, University of Brawijaya, Malang, Jl. Veteran,
Malang – East Java, Indonesia, 65145
3
Master Program of Aquaculture, Faculty of Fisheries and Marine Science, University of Brawijaya, Jl. Veteran,
4
Doctoral Program of Environmental Science, Postgraduate Program, University of Brawijaya, Jl. Veteran, Malang,
65145, Indonesia
*Corresponding author: doktoruun@ub.ac.id
INTRODUCTION. Viral Nervous Necrosis (VNN) is an RNA virus capable of infecting grouper larvae and
juveniles with a mortality rate of up to 100%. The alternative to deal with this problem is the administration
of adjuvant conjugated C. vulgaris nano-vaccines. Administration of recombinant nano-vaccine can also
stimulate fish growth through changes in fish length and weight [1]. This study aimed to determine the effect
of administering recombinant C. vulgaris nano-vaccine on grouper physiology and its potential anti-viral
activity. METHODS. The method used in this research is experimental. Water quality (temperature, pH, DO,
CO2, salinity, nitrate, ammonia and phosphate) was carried out in all treatment tanks was under controlled.
The treatments were (A) Healthy fish without treatment; (B) VNN-Infected fish; (C) fish infected with VNN
+ 33 µl recombinant vaccine C. vulgaris + 8.5 µl Chitosan nanoparticles; (D) VNN-infected fish + 66 µl
recombinant vaccine C. vulgaris + 16.5 µl Chitosan nanoparticles; (E) VNN-infected fish + 112 µl recombinant
vaccine C. vulgaris + 28 µl Chitosan nanoparticles. Confirmation of Interferon-gamma (IFN-ɣ) response using
PCR and growth observations by measuring the length and body weight of fish for 28 days. RESULTS AND
DISCUSSION. The results of the length and weight of grouper were negative allometric (b<3). The increase
in length is faster than the increase in weight. The results of PCR analysis showed that the fish in this study
that showed an IFN-ɣ response obtained a band at 500 bp or parallel to the positive control. The results of
water quality temperature parameters were obtained in the range of 27.7-30.20C, pH 7.1-8.2, dissolved oxygen
between 4-7 mg L-1, CO2 about 20-22 mg L-1, the salinity of about 29-32.5 g L-1, nitrate about 0.02-0.07 mg L-
1
, ammonia about 0.5-0.75 mg L-1, and phosphate about 0.07-0.25 mg L-1. CONCLUSION. The administration
of the recombinant C. vulgaris nano-vaccine affected the growth of the length and weight of cantang grouper
and also affected the IFN-ɣ response in each treatment so that it could be recommended as an alternative anti-
virus for grouper commodities.
REFERENCE
[1] Chojnacka, K., Skrzypczak, D., Izydorczyk, G., Mikula, K., Szopa, D., & Witek-Krowiak, A. (2021). Antiviral Properties of
Polyphenols from Plants. Foods, 10(10): 22-77.
Oral Presentation
IOT-Based Microbubble Aerator Application for Water

Quality Control in Catfish Farming at Mina Berkah Sejahtera
Farmers Community, Situbondo
Uun Yanuhar1*, Muhammad Musa1, Herly Evanuarini2, Aulia Fuad Rahman3, Dyah Kinasih
Wuragil4, Nur Sakinah Junirahma5 and Nico Rahman Caesar6
1
Aquatic Resources Management, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, Jl. Veteran,
Malang – East Java, Indonesia, 65145
2
Faculty of Animal Husbandry, Brawijaya University, Malang, Jl. Veteran, Malang – East Java, Indonesia, 65145
3
Faculty of Economics and Business, Brawijaya University, Jl. MT. Haryono No.165, Malang - East Java, Indonesia,
65300
4
Faculty of Veterinary Medicine, Brawijaya University, Puncak Dieng Eksklusif, Kalisongo, Malang - East Java,
Indonesia, 65151
5
Master Program of Aquaculture, Faculty of Fisheries and Marine Science, Brawijaya University, Jl. Veteran, Malang,
Indonesia, 65145
6
Doctoral program of Environmental Science, Postgraduate Program, Brawijaya University, Jl. Veteran, Malang,
Indonesia, 65145
*Corresponding author: doktoruun@ub.ac.id
INTRODUCTION. Intensive catfish farming can lead to poor media water quality, including low levels of
dissolved oxygen and high levels of organic waste, especially organic nitrogen. Keeping the water quality of
aquaculture ponds in good condition can be done by using Internet of Things (IoT)-based microbubble
technology, that water quality can be carried out in real-time via smartphones. This study aims to report
differences in water quality parameter values before and after the application of microbubbles in super
intensive catfish culture ponds. METHODS. The method used in this research is descriptive. The water quality
parameters studied were temperature, water brightness (cm), pH, Dissolved Oxygen (DO), Carbon Dioxide
(CO2), nitrate, orthophosphate, and Total Organic Matter (TOM). The measurement of water quality
parameters was carried out in catfish cultured ponds that did not use microbubbles and in catfish ponds with
aplication of microbubble. RESULTS AND DISCUSSION. The results of pond after microbubble application
was in temperature parameters is 30ºC, water brightness is 9.5 cm, pH 6.8, DO obtained 6.2 mg L-1, CO2
obtained 32.96 mg L-1, nitrate obtained 10.8 mg L-1, phosphate obtained 2.6 mg L-1, and TOM obtained 130.19
mg L-1. The results of pond after microbubble application was in temperature parameters obtained 28ºC, water
brightness obtained 9.5 cm, pH 8.3, DO obtained 6.5 mg L-1, CO2 obtained 9.4 mg L-1, nitrate obtained 0.532
mg L-1, phosphate obtained 0.9 mg L-1, and TOM between 23.8 mg L-1. Based on the results of the analysis of
water quality parameters, the parameter values in catfish culture ponds using microbubble technology have
improved and closer to the optimal value for catfish culture productivity. CONCLUSION. The use of
microbubbles has an impact on optimizing the value of water quality parameters in catfish culture ponds.
Oral Presentation
MortaparibMild- A novel small molecule inhibitor of mortalin

inhibits proliferation and migration of cancer cells by multiple
pathways
Shi Yang1,2, Hazna Noor Meidinna2, Anissa Nofita Sari2, Huayue Zhang1,
Sunil C Kaul2 and Renu Wadhwa2*
1
Graduate School of Life & Environmental Sciences, University of Tsukuba, Ibaraki 305-8575, Japan;
2
AIST-INDIA DAILAB, National Institute of Advanced Industrial Science & Technology (AIST), Tsukuba - 305 8565,
Japan. Email: s-kaul@aist.go.jp
*Corresponding author: renu-wadhwa@aist.go.jp
INTRODUCTION. Mortalin, a member of the Hsp70 family of proteins, enriched in many types of cancers,
has been shown to be involved in multiple ways in the process of carcinogenesis. It is known to inactivate
tumor suppressor protein p53 by its retention in the cell cytoplasm. Disruption of mortalin-p53 interaction and
reactivation of p53 has been shown to trigger growth arrest/apoptosis in cancer cells and hence, suggested to
be useful in cancer therapy. In this context, screening of a chemical library to identify potential abrogators of
mortalin-p53 interaction was performed, and two novel synthetic small molecules called Mortaparib and
MortaparibPlus have been reported, which were shown to possess anticancer activity that was mediated through
targeting mortalin and PARP1 proteins, essential for cancer cell survival and proliferation. Most recently,
another novel compound, (4-[(4-amino-5-thiophen-2-yl-1,2,4-triazol-3-yl)sulfanylmethyl]-N-(4-
methoxyphenyl)-1,3-thiazol-2-amine, isolated in the same screening was shown to be capable of interacting
with mortalin, p53, and PARP1. The compound was called MortaparibMild and is proposed to be a novel
member of Mortaparib class of anti-cancer compounds that warrants further experimental and clinical
attention. METHODS. We used cancer cells possessing wild type, mutant or null p53 status. Cell proliferation,
growth arrest, apoptosis, migration and invasion capacity of control and Mortaparibmild treated cells was
determined by biochemical, imaging and expressin analyses. RESULTS AND DISCUSSION. We found that
MortaparibMild can cause growth arrest or apoptosis of cancer cells by abrogating mortalin-p53 interaction
yielding reactivation of p53 function. It also causes inactivation of PARP proteins accounting for its anticancer
activity. Furthermore, decrease in mortalin and PARP1 expression was seen that are suggested to compromise
the migration capacity of cancer cells by multiple ways. CONCLUSION. Taken together, we report the
anticancer potential of MortaparibMild that warrants further attention in laboratory and clinical studies.
Oral Presentation
Alginate-Based Microencapsulation of Ruellia tuberosa L.

Ethanolic Extract and Their Activities as Alpha-Amylase
Inhibitor
Andriana Kusuma Pertiwi1, Zubaidah Ningsih1, and Anna Safitri1,2*
1
Chemistry Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, 65145,
Indonesia
2
Research Center for Smart Molecules of Natural Genetic Resources (SMONAGENES), Brawijaya University,
*Corresponding author: a.safitri@ub.ac.id
INTRODUCTION. The bioactive compounds naturally occurring in plants are important due to their
bioactive properties. The ethanolic extract of R. tuberosa L. is supposed to have an antidiabetic activity because
of the presence of flavonoid compounds. Nevertheless, these compounds are very unstable and have low
bioavailability. Microencapsulation is one of the shielding techniques to protect and increase the stability of
bioactive compounds. This work used sodium alginate and calcium chloride as shell materials. METHODS.
Microcapsules of R. tuberosa L. extracts were prepared using a freeze-drying method by varying the acetate
buffer pH (4, 5, 6, and 7), sodium alginate concentration (1; 1.5; 2; and 2.5% (w/v)), and stirring time (15, 30,
45, and 60 minutes). The optimum conditions of microcapsules were determined from the highest percentage
of encapsulation efficiency (%EE). The activity of optimum microcapsules was then tested using the alpha-
amylase inhibition assay. Moreover, the characterization was also carried out using FTIR, SEM, and PSA
instruments. Lastly, the release behaviour of microcapsules was conducted on two different pHs, pH 1.2 and
pH 7.4, with times release of 30, 60, 90, and 120 minutes. RESULTS AND DISCUSSION. The optimum
conditions of microcapsules were acquired at pH 6, with 1% (w/v) of alginate concentration, and 30 min
stirring time (51.63 ± 0.09% EE). The alpha-amylase inhibition assay from microcapsules yielded high activity
with the IC50 value of 46.66 ± 0.13 mg/mL. The FTIR spectrum showed that cross-linking between sodium
alginate and CaCl2 was obtained from alginate’s C-O ester band in 1036.68 cm-1. Characterization with SEM
and PSA denoted that microcapsules had a few irregular and spherical shapes, with a mean diameter of 197.53
μm. The bioactive compounds released at pH 1.2 in 120 min was only 3.51%, while at pH 7.4 resulted in a
higher amount of 55.78%. CONCLUSION. This work confirms that the obtained microcapsules may be used
to develop plant extracts with their activities as the alpha-amylase inhibitor.
REFERENCE
[1] M. Ramadhan, A. Sabarudin, A. Safitri A, "In Vitro anti-microbial activity of hydroethanolic extracts of Ruellia tuberosa L.: Eco-
friendly based-product against selected pathogenic bacteria", IOP Conference Series: Earth and Environmental Science, vol. 239,
Article ID 012028 (2019).
[2] A. Safitri, A. Roosdiana, I. Rosyada, C. Evindasari, Z. Muzayyana, and R. Rachmawanti, “Phytochemicals screening and anti-
oxidant activity of hydroethanolic extracts of Ruellia tuberosa L,” IOP Conference Series: Materials Science and Engineering, vol.
509, p. 012017 (2019)
[3] A. Nandiyanto, R. Oktiani, and R. Ragadhita, “How to Read and Interpret FTIR Spectroscope of Organic Material,” Indonesian
Journal of Science and Technology, vol. 4, pp. 97–118 (2019)
[4] O. Abbas, G. Compère, Y. Larondelle, D. Pompeu, H. Rogez, and V. Baeten, “Phenolic compound explorer: A mid-infrared
spectroscopy database,” Vibrational Spectroscopy, vol. 92, pp. 111–118 (2017)
Oral Presentation
Effect of acetic acid in inhibition Pseudomonas aeruginosa

biofilm in chronic suppurative otitis media (in vitro study)
Artono,1 Sukma Nisa Janitra,2 Nyilo Purnami2, Edi Handoko3 and Agung Dwi Wahyu
Widodo4
1
Doctoral Program, Faculty of Medicine, Universitas Airlangga, Dr. Moestopo Road no. 47, Surabaya
60285, Indonesia
2
Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Airlangga
Dr Soetomo General Hospital. Dr Moestopo Road no. 47, Surabaya 60285, Indonesia
3
Department of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Brawijaya,
Veteran Malang Road, Malang 65145, Indonesia
4
Department of Clinical Microbiology, Faculty of Medicine, Universitas Airlangga Dr Soetomo General
Hospital. Dr Moestopo Road no. 47, Surabaya 60285, Indonesia
Correspondence : Nyilo Purnami; nyilo@fk.unair.ac.id
Background: The high incidence of Chronic Suppurative Otitis Media (CSOM) is caused by multifactorial.
One of them is the ability of pathogenic bacteria to form biofilms. The formation of biofilms on bacteria can
inhibit the penetration of antimicrobial agents and avoid the immune defense system. It becomes one of the
factors causing therapy failure and chronicity of infection. Pseudomonas aeruginosa is the most common
bacteria found in CSOM which has the virulence ability to form biofilm structures. A number of studies have
reported that acetic acid has the ability to inhibit and eradicate biofilm complexes and is thought to be an
alternative to additional therapy against bacterial infections that form biofilms..
Objective:
Explain the effect of acetic acid in inhibiting Pseudomonas aeruginosa biofilm in CSOM.
Methods:
This study used an experimental in vitro laboratory with a post-test-only control group method. Samples of
Pseudomonas aeruginosa were taken from the secretions of the mastoid cavity of CSOM patients. The
inhibitory effect of acetic acid was observed by administering acetic acid to Pseudomonas aeruginosa. The
concentrations of acetic acid used were 0.04%, 0.08%, 0.16%, 0.31%, 0.63%, 1.25%, 2.5% and 5%. The
observations in this study were using the microtiter plate assay method and were measured with an ELISA
reader. Data analysis used the One Way Anova test and multiple comparisons (Tukey HSD Test).
Result:.
The inhibitory effect of acetic acid on the growth of Pseudomonas aeruginosa biofilm was obtained (p=0.000)
with significant results (p <0.05) between the positive control group and the concentration group of 0.16%,
0.31%, 0.63%, 1.25. %, 2.5%, and 5%. The Minimum Biofilm Inhibitory Concentration (MIBC) value of
acetic acid in the formation of Pseudomonas aeruginosa biofilms was 0.16%.
Conclusion :
Acetic acid has an effect on inhibiting the formation of Pseudomonas aeruginosa biofilms in CSOM.
Keyword: Chronic suppurative otitis media (CSOM), Biofilm, Pseudomonas Aeruginosa, Acetic Acid,
Human Disease
Oral Presentation
Medication Adherence Outpatients Type 2 Diabetic Mellitus

at Four Primary Health Care Centers in Medan City,
Indonesia
Embun Suci Nasution1*, Urip Harahap1, Baharudin Ibrahim2 and Khairunnisa1
1
Departement of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
2
Department of Clinical Pharmacy, Faculty of Pharmacy, University of Malaya, Kuala Lumpur, Malaysia.
*Corresponding author: urip@usu.ac.id and embun@usu.ac.id
INTRODUCTION. Diabetic mellitus is considered as ”the mother of all diseases" because it causes many
complications. Knowing and measuring medication adherence may have a greater effect on diabetic patients.
Several studies in Indonesia used a questionnaire scale to measure adherence, however they do not validate
the study population, so it could still be found anomalous correlation analysis between adherence and clinical
data even though it measured in the same country and scale. METHODS. This study measures the adherence
level of type 2 diabetes patients, evaluates the validity of the medication adherence scale, and analyze the
correlation with the clinical outcome of type 2 diabetes patients in four primary health centers in Medan City,
Indonesia. The study uses a cross-sectional design in Prolanis type 2 diabetic mellitus patients of November-
December 2021 and the MARS-5 questionnaire is valid and reliable. RESULTS AND DISCUSSION. 89 of
Pronalis type 2 diabetic mellitus patients from four primary health care centers, the measurement showed
89.1% was adherent patients and 10.1% was non-adherent patients. Correlation analysis showed that there was
significant relationship (p> 0.05) between patient adherence and clinical outcome. CONCLUSION. Those
results showed that type 2 diabetes mellitus patients in four primary health centers were categorized as adherent
and correlated with the clinical outcome.
Keyword: diabetic mellitus, adherence, outpatients, primary health care center
REFERENCE
[1] Alfian SD, Sukandar H, Lestari K, Abdullah R. Medication adherence contributes to an improvedquality of life in type 2
diabetes mellitus patients: a cross-sectional study. Diabetes Ther.2016:7(4):755-64.
[2] Alfian SD, Sukandar H, Arisanti N, Abdulah R. Complementary and alternative medicine use decreases adherence to
prescribed medicationin diabetes patients. Ann Trop Med Public Health. 2016;9(3):174-9.
[3] Acik, Y., Hulya, Y. B., Canan, G., Ozge, A., and Nevin I. (2004). Effectivenes of A Diabetes Education And Control
Intervention Program On Blood Glucose Control For Patient With Type 2 Diabetes In A Turkish Community. Southeast
Asian J Trop Med Public Health. 35(4):1012-1018.
[4] Agrimon, O. H. (2014). Exploring The Feasibility of Implementing Self- Management and Patient Empowerment Through A
Structured Diabetes Education Pragramme in YogyakartaCity Indonesia : A Pilot Cluster Randomised Controlled Trial.
Thesis. Adelaide: Dicipline ofGeneral Practice and Dicipline of Public Health School of Population Health. Faculty of
Health Sciences The Unversity of Adelaide.
[5] Cani CG, Lopes LS, Queiroz M, Nery M. Improvement in medication adherence and self- management of diabetes with a
clinical pharmacy program: a randomized controlled trial in patients with type 2 diabetes undergoing insulin therapy at a
teaching hospital. Clinics. 2015;70(2): 102–106.
[6] International Diabetes Federation. IDF Diabetes Atlas-Eight edition 2017. 8th ed. International Diabetes Federation. 2017.
Krapek K, King K, Warren SS, George KG, Caputo DA, Mihelich K, et al. Medication adherenceand associated hemoglobin
A1c in type 2 diabetes. Ann Pharmacother. 2004;38(9);1357-62. Krass I, Schieback P, Dhippayom T. Adherence to diabetes
medication: a systemic review. Diabet Med. 2015;32(6):725-62.
[7] World Health Organization. Diabetes country profile. 2016-
Indonesia. WorldOragnization.https://www.who.intl/diabetes/country-profile/idn_en.pdf?ua=1.
[8] Wibowo Y, Parson R, Sunderland B, Hughes J. An evaluation of community pharmacy-basedservices for type 2 diabetes in
an Indonesian setting: patient survey. PerrJ.2015:3:e1449.
Oral Presentation
Anthocyanin From Purple Sweet Potatoes Modify Visceral Fat

Tissue Inflammation and Hepatic Stress Oxidative in Chronic
Restraint Stress-Induced Mice
Nia Kurnianingsih1,3*, Dian Artamevia2, Antania Kashira Winarta2, Andini Putri
Wulandari2, Syifa Insiya Siregar2, Dian Hasanah1 Edwin Widodo1, Retty Ratnawati1
1
Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
2
Bachelor Program of Medicine, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
3
Research Centre of Smart Molecules of Natural Genetics Resiources Universitas Brawijaya, Malang, Indonesia
*Corresponding author: nia_fkub@ub.ac.id
INTRODUCTION. Stress has prominent adverse effect on psychopathological changes and metabolic
diseases. The systemic consequences of stress are strongly correlates with tissue stress oxidative and
inflammation. The application of plant metabolite compound is promising to overcome these circumstances.
Several plant anthocyanins previously showed favourable effect as powerful antioxidant and anti-
inflammatory properties. However, the effect of anthocyanin (ANC) from purple sweet potatoes on stress is
lacking and need further investigation. Thus, this study was purposed to explore the effect of total anthocyanin
from purple sweet potatoes on inflammation and stress oxidative of chronic restraint stress-induced mice
model. METHODS. A total of 25 male adult mice at a age of 7-8 weeks were randomly assigned into 5 group
consist of control, stress, stress+ANC 10mg/BW, stress+ANC 20mg/BW and stress+ANC40mg/BW. Stress
was exposed as immobilitation stress 2 hours/day for 14 days. Total ANC extract was administrated intragastric
once per day. The level of interleukin-6 (IL-6) and IL-10 from visceral fat tissue as well as hepatic superoxide
dismutase (SOD) and malondyaldehide (MDA) were analysed using enzyme-linked immunoassay. The
ANOVA statistic was performed with the level of significant was set as p<0,05. RESULTS AND
DISCUSSION. Stress is dramatically increases the level of IL-6. Anthocyanin is significantly lowering the
level of IL-6 (p=0,005). Meanwhile the combination of stress and ANC is tend to increase the level of IL-10
in 20 mg/BW of ANC group. Despite the level of MDA has no difference among groups, the level of SOD is
significantly reduce among combination of stress and ANC treatments. We suggest the modifying effect of
anthocyanin on stress-induce inflammation in visceral tissue and attribute as antioxidant in hepatic tissue
CONCLUSION. The administration of total anthocyanin extract of purple sweet potatoes is reduce visceral
fat inflammation and tend to inhibit the stress oxidative in hepatic tissue of chronic restraint stress-induced
mice. Further studies are necessary to evaluate further these effect of behavioral measurements.
REFERENCE
[1] Kotera, et al., International Journal of Mental Health and Addiction. (2021).
[2] Bergmann, et al, Endocrine Connetions, 3(2) (2014).
[3] Settapramote, et al, Molecules, 26, 4978 (2021)
[4] Reyhanditya, et al, Jurnal Kedokteran Brawijaya 32(1) (2022)
[5] Kurnianingsih, et al., Systematic Rev. in Pharm, 11(10) (2020)
Oral Presentation
Correlation Between p53 and Ki67 with Aggressiveness Factor

in Recurrent Respiratory Papillomatosis
Rizka Fathoni Perdana1, Sri Herawati2*, Muhtarum Yusuf3, Puguh Setyo Nugroho4
1
Doctoral Program, Faculty of Medicine, Universitas Airlangga, Dr. Moestopo Road no. 47, Surabaya 60285,
Indonesia, email : rizka-f-p@fk.unair.ac.id
2
Departemen of Otorhinolaryngology Head and Neck Surgery, Faculty of Medicine, Universitas Airlangga, Dr.
Moestopo Road no. 47, Surabaya 60285, Indonesia, email : sri-h-j@fk.unair.ac.id
3
Moestopo Road no. 47, Surabaya 60285, Indonesia, email : muhtarum.yusuf@fk.unair.ac.id
4
Moestopo Road no. 47, Surabaya 60285, Indonesia, email : puguh-s-n@fk.unair.ac.id
*Corresponding author: Sri Herawati. Email : sri-h-j@fk.unair.ac.id
INTRODUCTION. RRP is a benign papilloma tumor that grows in the upper respiratory tract and is mainly
caused by HPV types 6 and 11. The HPV activities during neoplasm pathogenesis are associated with a
complex cell cycle that causes cell replication and growth. The p53 and Ki67 proteins are tumor gene
controllers that regulates the cell cycle and helps during the process of DNA apoptosis. METHODS. This
research is an analytical observational study with a cross-sectional approach. An immunohistochemical
method of staining the monoclonal antibody rabbit Anti-Human p53 and Ki67 clone 318-6-11 is used to
examine the p53 and Ki67 proteins. The aggressiveness of the disease was determined by evaluating the
medical records during treatment at Dr. Soetomo Hospital, Surabaya. RESULTS AND DISCUSSION. Out
of nineteen samples, there are more women (57.90%) than men, with the juvenile type of RRP dominating
(68.40%). There are 12 patients (63,20%) with the aggressive disease course compare with 36,80% indolen
dissease. Positive p53 protein can be obtained in 8 (42.19%) patients. In comparison, the Ki67 examination
found 5 (26.03%) patients with positive results. High p53 protein expression indicates an uncontrolled cell
cycle. It is known that the p53 protein is the controller of the apoptotic process. The uncontrolled cell cycle
causes tumors to grow rapidly. High levels of Ki67 expression indicate an uncontrolled cell cycle because
most cells are in a dormant state. CONCLUSION. There was no correlation between the aggressiveness of
RRP disease and the degree of expression of protein p53 and Ki67. Factors that may have a significant role
in the aggressiveness of RRP disease are the type of RRP (juvenile type being more aggressive than adult)
and HPV (type 11 is more aggressive than 6).
REFERENCE
[1] Buchinsky FJ, Valentino WL, Ruszkay N, Powell E, Derkay CS, Seedat RY, et al. Age at diagnosis, but not
HPV type, is strongly associated with clinical course in recurrent respiratory papillomatosis. PLoS One 2019;14(6)
[2] Donne AJ, Clarke R. Recurrent respiratory papillomatosis: an uncommon but potentially
devastating effect of human papillomavirus in children. Int J STD AIDS. 2010;21(6):381–5
[3] Perdana RF, Herawati S, Surarso B, Aksono EB. Correlation of Aggressivity Papilloma Recurrent
Respiratory Tract With Human Papillomavirus Types 6 And 11. Med Leg Updat. 2020;20(1):471–6
[4] Foster I. Cancer: A cell cycle defect. Radiography. 2008;14(2):144–9
[5] Ganguly N, Parihar SP. Human papillomavirus E6 and E7 oncoproteins as risk factors for
tumorigenesis. J Biosci. 2009;34(1):113–23.
[6] Ghittoni R, Accardi R, Hasan U, Gheit T, Sylla B, Tommasino M. The biological properties of E6 and
E7 oncoproteins from human papillomaviruses. Virus Genes. 2010;40(1):1–13.
[7] Wiatrak BJ, Wiatrak DW, Broker TR, Lewis L. Recurrent respiratory papillomatosis: a longitudinal
study comparing severity associated with human papilloma viral types 6 and 11 and other risk factors
in a large pediatric population. Laryngoscope. 2004;114(S104):1–23.
[8] Stern Y, Mueller K, Willging JP, Myer CM, Cotton RT. S-phase fraction as a predictor of prognosis in juvenile respiratory papillomatosis. Arch
Otolaryngol Neck Surg. 1998;124(5):541–4
[9] Bonagura VR, Vambutas A, DeVoti JA, Rosenthal DW, Steinberg BM, Abramson AL, et al. HLA alleles, IFN-γ responses to HPV 11 E6, and
disease severity in patients with recurrent respiratory papillomatosis. Hum Immunol. 2004;65(8):773–82
[10] DeVoti JA, Steinberg BM, Rosenthal DW, Hatam L, Vambutas A, Abramson AL, et al. Failure of gamma interferon but not interleukin-10
expression in response to human papillomavirus type 11 E6 protein in respiratory papillomatosis. Clin Diagn Lab Immunol. 2004;11(3):538–47.
Oral Presentation
Metabolite Profiles and Hypoglycemic Activities of Seed

Extract of Pinang (Areca catechu L.)
T Suryowati1*, Forman E. Siagian2, R A K Putri3
1*
Department of Biochemistry, Medical College, Universitas Kristen Indonesia, trini.suryowati@uki.ac.id
2
Department of Parasitology, Medical College, Universitas Kristen Indonesia, forman.siagian@uki.ac.id
3
Medical Student, Medical College, Universitas Kristen Indonesia, rinikusumaputri@gmail.com
*Corresponding author: trini.suryowati@uki.ac.id
INTRODUCTION. Pinang (Areca catechu L.) fruit is edible as local indigenous plant from West Irian Jaya
(Papua) Indonesia. The aim of this work is to analyze antidiabetic activity and characterize its biological active
compounds (glycosidase inhibitory) and their specific properties, including antioxidant properties.
METHODS. It is an experimental study to determine the chemicals compound in seeds and leaves by GC-MS
technique and the screening for the potent natural glycosidase inhibitory. Place and Duration of Study: Medical
Faculty of Universitas Kristen Indonesia, between December 2021 to March 2022. RESULTS AND
DISCUSSION. The bioactive compound evaluation of Pinang seeds confirmed the presence of: Piperazine,
2-methyl- (CAS) 2-Methylpiperazine (16.70 %); 16.04 Tetrahydro-4H-pyran-4-ol (16.70%); 1,4-Butanediol
(CAS) Sucol B (9.72%); Nickel 1-amino-1,9 Diisothiocipno- 4,8-Diazaundecamine-Azaundecamine (9.38%);
6H-Pyrazolo[1,2-a][1,2,4,5] tetrazine, hexahydro-2,3-dimethyl- (CAS) 1,3,4,6-Tetraazabicyclo (7.895). The
analysis of Pinang fruits revealed the presence of Trans- 3,5-Dideutero Hygroxy Cyclopentene (13.13%); 6.86
3- Methyl-Oxirane-2-Carboxylic Acid (6.86%); 5.73 N-Ethyl-N-Methyl-3-Butenylamine (5.73%); 1H-Pyrrole
(CAS) Pyrrole (5.44%); 1,2-(1'-Methyltrimethylene)-Diborane (5.24%); Vitamin E (0.20 %). The analysis of
Pinang leaves revealed the presence 6H-Pyrazolo[1,2-a][1,2,4,5]tetrazine, hexahydro-2,3-dimethyl- (CAS)
1,3,4,6-Tetraazabicyclo (6.28%); Cyclohexanone, 3-hydroxy- (CAS) 3-Hydroxycyclohexanone (5.25%);
Pregeijerene (5.22%); Silanediamine, 1-chloro-N,N,N',N',1-pentamethyl- (5.08%); Butanal, 3-methyl- (CAS)
3-Methylbutanal (4.82%); Vitamin E (1. 38%). IC50 values inhibition of α-glucosidase seeds extract was 82.74
ppm and glucobay standart was 0.34 ppm. CONCLUSION. This study confirmed that Pinang seeds contain
glucosidase activity that have the potency to inhibits glucose and potentially responsible for health benefits.
Further study is required to reveal its biological effects on cell lines or even on animal models.
REFERENCE
[1] You Q et al., J. Agric. Food Chem. 59:9506-9511 (2011)
[2] Yin Z et al., Food Sci. Hum. Wellness. 3:136-174 (2014)
Oral Presentation
Correlation between Serum Levels of Matrix

Metalloproteinases 1 and 9 with Cavity and Fibrosis in
Multidrug-Resistant Subjects Using High-Resolution
Computerized Tomography of the Thorax
Anse Diana Valentiene Messah
Biomedical Science Daculty of Medicine, University of Indonesia
Matrix metalloproteinases (MMPs) are proteins that play a role in inflammation and remodeling processes
caused by infections, including pulmonary tuberculosis (TB). This research aims to determine the relationship
between serum levels of MMP-1 and MMP-9 with cavities characteristics (number, diameter, wall thickness)
and distribution of fibrosis and compare in multidrug-resistant (MDR) and drug-sensitive patients. (DS) TB.
This research uses a cross-sectional comparative study design and correlation study. The topic is from
outpatients at Abdoel Moelok Hospital, Lampung Province from April to December 2021, who have passed
the ethical test. A total of 70 subjects were divided into two groups, 34 subjects in the MDR-TB group and 36
subjects in the DS-TB group. MMP-1 and MMP Level levels were carried out by ELISA test. In addition,
cavities and fibrosis were measured using the thorax High-Resolution Computerized Tomography (HRCT)
Imaging. More cavities than 3, the cavity is 3 mm in diameter, and the thickness of the SS cavity is 2.4 mm
more often in HRCT MDR-TB than in DS-TB. Although MMP-9 levels in the MDR-TB group were the
highest compared to the DS-TB group, there was no statistically significant difference in cavities
characteristics and distribution of fibrosis in the lung segments between MDR-TB and DS-TB subjects. This
study showed that cavity features (number, diameter, and wall thickness) were more common in MDR-TB
patients than in DS-TB patients. However, serum levels of MMP-1 and MMP-9 did not contribute to cavity
characteristics and distribution of fibrosis in MDR-TB and DS-TB.
Keywords: Multidrug resistance Tuberculosis, Cavity, Fibrosis, MMP-1, MMP-9, and High-Resolution
Computerized Tomography
Oral Presentation
SLEEP DISTURBANCES SCREENING AMONG

ADOLESCENTS IN BATU, EAST JAVA, INDONESIA
Novita Titis Harbiyanti1*, Aris Widayati1, Dian Hasanah1, Edwin Widodo1,
Agwin Fahmi F1, Retty Ratnawati1, Endang Sriwahyuni1, Nia Kurnianingsih1
Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
*email : dr.novitatitis@ub.ac.id
Sleep disturbances are common among adolescents and have prominent adverse impacts on their daily
activities such as cognitive deficit, attention problems, physical development impairment and emotional
disturbance as well as social function. However, sleep disturbances among adolescents frequently delayed
recognition thus resulting in inadequate treatment. This study aimed to screen the profiles of sleep disturbances
among adolescents in Batu, East Java, Indonesia. A total of 33 adolescents at the age of 16-18 were involved
in this descriptive study. A set of questionnaires that consist of Insomnia Severity Index Indonesian version
and Epworth Sleepiness Scale in Indonesian version were utilized for screen insomnia and hypersomnia
respectively. All the participants were students in Vocational High School, 91% of participants were female.
Insomnia was prevalent in female participants. Moderate insomnia identified in 15,2% female participants and
none of male participants. Mild insomnia identified in 54,5% female participants and 6,1% male participants.
Hypersomnia was identified in 33,3% participants consisting of 90,9% female and 9,1% male. We concluded
that sleep disturbances of insomnia are common in females. Hypersomnia is also found more frequently in
females. Further investigations are necessary to evaluate the effect of these disturbances in adolescent’s quality
of life .
Keywords : Sleep disturbance, adolescents, insomnia
Oral Presentation
Microencapsulation of Ethanolic Leaves Extract of Cosmos

caudatus Kunth Using Gum Arabic in various pH and Extract
concentration.
Anna Roosdiana 1*, Anna Safitri2, Winda Ayu Dwi SetyoNingsih3 and Sutrisno4
1
Faculty of Mathematics and Natural Sciences, Brawijaya University , aroos@ub.ac.id
2
Faculty of Mathematics and Natural Sciences, Brawijaya University a.saftri@b.ac.id
3
Faculty of Mathematics and Natural Sciences, Brawijaya University ayudwiwinda@ub.ac.id
4
Faculty of Mathematics and Natural Sciences, Brawijaya University tris_mc@ub.ac.id
*Corresponding author: aroos@ub.ac.id
INTRODUCTION. Cosmos caudatus Kunth contains flavonoid compounds which can act as antioxidant and
easily affected by surrounding environment. The flavonoid of Cosmos caudatus Kunth might be used as
therapy agent for cancer and diabetic diseases . An alternative process to protect the flavonoids can be done
through microencapsulation that can coat small particles of bioactive compounds. This study aimed to
determine the effect of pH and concentration of ethanolic leaves extract of C. caudatus K on
microencapsulation using gum arabic as coating agent and to investigate its antioxidant activity METHODS.
In this study the pH was applied 3, 4, 5, and 6, while the concentration of ethanolic leaves extract of C.
Caudatus K were 0.1%; 0.2%; 0.3%; 0.4%; and 0.5% (w/v). The microencapsulation efficiency was used to
determine the optimum conditions .The resulted microcapsule was identified by using FTIR spectrophotometer
and tested for its antioxidant activity using FRAP (ferric reducing antioxidant power ) method. RESULTS
AND DISCUSSION. Results showed that pH 5 and 0.2 % of concentration of ethanolic leaves extract of
C. Caudatus K indicated the highest microencapsulation efficiency of 79.92%. The FTIR spectra showed
absorption at wave numbers of 3385.83 Cm-1 (OH), 2935.14 Cm-1 (CH3 alkane), 1603.06 cm-1 (C=C aromatic),
1571.69 Cm-1 and 1471.65 Cm-1 (carboxylic acid), and 1143.82 cm-1 (C-O alcohol) which approved the
formation of microcapsules from ethanolic leaves extract of C. caudatus K in gum arabic. The microcapsules
had antioxidant activity with the Ic50 value of 66.56 ± 1.25 µg/ml. CONCLUSION. The microencapsulation
of ethanolic leaves extract of C. caudatus K in gum arabic has been successfully done. The optimum
condition of microencapsulation was occurred at pH 5 and 0.2% of ethanolic leaves extract of C. caudatus
that showed high antioxidant activity with Ic50 value of 66.56 ± 1.25 µg/ml . However to apply the
microcapsules of C. Caudatus extract as an alternative therapy for diabetes , further study should be done
for in vivo its biological activity such as antioxidant and amylase inhibition activities. In addition ,the extract
released from microcapsules in pH digestive system is also necessary observed.
Figure 1. FTIR spectra of microcapsule of C.caudatus K extract, gum arabic and

C.caudatus K extract
REFERENCE
[1] A. Mediani, F. Abas, A. Khatib, and C. Tan, Molecules. 18, 10452–10464 (2013)
[2] A. Safitri, A. Roosdiana, E. Hitdatania, and S. A. Damayanti , Indones. J. Chem . 22,212( 2021)
[3] V. Perumal, A. A. Hamid, A. Ismail, K. Saari, F. Abas, and I. S. Ismail, J.Food Chem. Nutr.1, 43-51(2014)
[4] A. Adam, Food Hydrocolloids, 126, 107451(2022)
Oral Presentation
Development of Smart Deploy Web Big Data App for

Determining Modeling of Medicinal Compounds to Healing
Various Disease Especially for Covid-19 and Its New Variants
Using Quantum Meta-Deep AI Algorithm
Imam Cholissodin1,*, Lailil Muflikhah2, Sutrisno3, Arief Andy Soebroto4, Aurick Yudha
Nagara5, Tamara Gusti Ebtavanny6, and Widodo7
1,2,3,4
Faculty of Computer Science, Computer Science, Brawijaya University, Malang, Indonesia,
{imamcs, lailil, trisno, ariefas}@ub.ac.id
5,6
Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia, {aurickyudha.fk, tamara.gusti}@ub.ac.id
7
Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Malang, Indonesia, widodo@ub.ac.id
*Corresponding author: (imamcs@ub.ac.id)
The global Covid-19 pandemic has shown signs of becoming endemic. This is encouraging news and also
become very big challenge, due to the many new variants of Covid-19 that have not been resolved quickly,
for example to determining model of medicinal compounds. These many variants also require more powerful
of computational than only 1 variant like in the previous cases. In this study, an approach was developed
using the Quantum meta-Deep AI algorithm to overcome the complexity and difficulty of building the
model. The steps of this algorithm are proposed by starting with Quantum Deep Learning (QDL), then
included in the meta-heuristic algorithm, namely Parallel Time-Variant Particle Swarm Optimization
(PTVPSO) based on Deep Extreme Learning Machine (Deep ELM). Optimization is carried out by taking
several parameters from the native QDL to be optimized in the form of several scenarios, namely using a
virtual Quantum RAM (QRAM) approach, namely without implementation on physical hardware or trying
physical ones and finally using a hybrid concept between virtual in binary QRRAM and physical. Then the
smart deploy technique of the web big data app was also developed as a place for the manufacture and
determination of drug candidate models for various diseases, especially Covid-19 and its new variants. Based
on the scheme of preparing the scenario, it is hoped that the results of the test will obtain a stable model for
the interaction of healing and accelerating health recovery as well as prevention in the human body where it
is endemic or when there are indications that a new virus reappears, the system quickly provides better
model recommendations.
Keywords: smart deploy, web big data app, modeling, various diseases, virus, covid-19, new variant, medicinal
compounds, quantum meta-deep ai algorithm
REFERENCE
[1] Amin, J., Sharif, M., Gul, N., Kadry, S., & Chakraborty, C. 2021. Quantum Machine Learning Architecture for COVID-19
Classification Based on Synthetic Data Generation Using Conditional Adversarial Neural Network. Cognitive computation, 1–12.
Advance online publication. https://doi.org/10.1007/s12559-021-09926-6
[2] Bradbury, J., 2018. Automating Software Development Using AI. Faculty of Science (Computer Science) University of Ontario
Institute of Technology Oshawa, Ontario, Canada.
[3] Cholissodin, I., Sutrisno, S., Santoso N., Soebroto, A.A., Hidayat N., dan Rochman N. T., 2020. Smart Development of Big Data
App for Determining the Modelling of Covid-19 Medicinal Compounds Using Deep AI Core Engine System. J. Phys.: Conf. Ser.
1665 012003 Published under licence by IOP Publishing Ltd
[4] Kemenkopmk, 2021. Pemerintah Siapkan Langkah Pandemi Covid-19 Menjadi Endemi.
https://www.kemenkopmk.go.id/pemerintah-siapkan-langkah-pandemi-covid-19-menjadi-endemi diakses 24 April 2022.
[5] Kemkes, 2021. Penguatan Sistem Kesehatan dalam Pengendalian COVID-19. http://p2p.kemkes.go.id/penguatan-sistem-
kesehatan-dalam-pengendalian-covid-19/ diakses 24 April 2022.
[6] Khan, T. M., dan Robles-Kelly, A., 2020. Machine Learning: Quantum vs Classical. in IEEE Access, vol. 8, pp. 219275-219294,
doi: 10.1109/ACCESS.2020.3041719.
[7] Maayan, G. D., 2021. Best of 2021 – 7 Popular Open Source CI/CD Tools. https://devops.com/7-popular-open-source-ci-cd-tools/
diakses 24 April 2022.
[8] Pittet, S., 2021. Continuous integration vs. delivery vs. deployment. https://www.atlassian.com/continuous-
delivery/principles/continuous-integration-vs-delivery-vs-deployment diakses 24 April 2022.
[9] Putri, S. A. E., 2022. Varian-varian Covid-19, Apa Perbedaannya?. https://corona.jakarta.go.id/id/artikel/varian-varian-covid-19-
apa-perbedaannya diakses 24 April 2022.
[10] Siddiqi, R., 2018. Is artificial intelligence related to software engineering?. https://www.quora.com/Is-artificial-intelligence-
related-to-software-engineering diakses 11 Maret 2020.
[11] Russell, S., dan Norvig, P., 2003. Artificial Intelligence: A Modern Approach. International Edition, Edisi 2. Pearson
Prentice-Hall Education International. New Jersey.
[12] Quora Contributor., 2018. Will Machine Learning Take Over Computer Science Jobs?.
https://www.forbes.com/sites/quora/2018/04/23/will-machine-learning-take-over-computer-science-jobs/#1e1074e950e9 diakses
11 Maret 2020. © 2020 Forbes Media LLC. All Rights Reserved.
[13] Zhao, Y., Huang, J., Zhang, L., Chen, S., Gao, J., Jiao, H., 2022. The global transmission of new coronavirus variants.
Environmental Research, Volume 206, 112240, ISSN 0013-9351, https://doi.org/10.1016/j.envres.2021.112240.
[14] Cholissodin, I., Sutrisno, S., Soebroto, AA., Hasanah, U., Febiola, YI., 2020. AI, Machine Learning & Deep Learning,
Fakultas Ilmu Komputer, Universitas Brawijaya, Malang.
[15] WHO, 2022. Genomic sequencing of SARS-CoV-2: a guide to implementation for maximum impact on public health.
https://www.who.int/publications/i/item/9789240018440 diakses 7 April 2022.
[16] WHO, 2022. Status of COVID-19 Vaccines within WHO EUL/PQ evaluation process (20 January 2022).
https://extranet.who.int/pqweb/sites/default/files/documents/Status_COVID_VAX_20Jan2022_v2.pdf diakses 7 April 2022.
Oral Presentation
Detection of Myxobolus sp. infection (Myxosporeasis) on

Cultured Koi fish in Blitar District, East Java Province
Muchammad Yunus1*, Agus Sunarso2, and Agus Wijaya3
1, 2
Veterinary Parasitology Division, Department of Veterinary Science, Faculty of Veterinary Medicine, Airlangga
University
3
Veterinary Internal Medicine Division, Department of Clinic, Reproduction and Pathology, Faculty of Veterinary
Medicine, IPB University
*Corresponding author email: muchammad-y@fkh.unair.ac.id
Abstract. Koi fish is one of the popular freshwater, ornamental fish throughout the world. Koi fish is
commercial fish that have high economic value currently being cultivated in Blitar District, East Java Province.
Myxozoa is economically and ecologically important group of metazoan parasites. Several myxozoa are an
important parasitic protozoan of freshwater fishes reported from almost all over the world. Koi fish can also
be infected one of myxozoa parasites. The severity of infection may lead to mortality of fish host. Observation
of the koi fish that became the sample and target of the study was carried out by observing clinical signs and
continued with macroscopic and microscopic observations. Parasite microscopically examination carried out
by combined conventional methods, native fresh mount then confirmed with histological section with HE
staining. While the analysis of the observation data was presented and explained descriptively. Koi fish gill
showed swelling and the presence of white nodule (plamodium) and it caused opened gill operculum.
Morphological observations showed the characteristics of plasmodium in which there were many spores that
characterized the Myxozoa group, especially Myxobolus sp. (Myxozoa: Myxosporea, Bivalvulida,
Myxobolidae). In detail plasmodium can be seen that many mature spores appeared pear-shaped in frontal
view and each spore contains two polar capsules and a sporoplasm. Mature spores measuring 12.51 – 14.72
µm in length and 7.07 – 8.97 µm in width. Two polar capsules were pyriform, polar capsule was between 7.7
– 8.95 µm in length and 2.65 – 3.58 µm in width containing 5–6 turns of polar filaments.
Keywords: Detection, Koi fish, Morphology, Myxobolus sp,
Oral Presentation
The Molecular Prediction of Indonesian Medicinal Plants'

Activity on 5-HT2a Receptor Involved in Schizophrenia
Wike Astrid Cahayani1*, Dearisa Surya Yudhantara2, and Nia Kurnianingsih3, 4
1
Department of Anatomy and Histology, Faculty of Medicine, Universitas Brawijaya, Indonesia
2
Department of Psychiatry, Faculty of Medicine, Universitas Brawijaya/ dr. Saiful Anwar Hospital, Indonesia
3
Department of Physiology, Faculty of Medicine, Universitas Brawijaya, Indonesia (nia_fkub@ub.ac.id)
4
Research Center of Smart Molecule and Natural Genetics Resources, Universitas Brawijaya, Indonesia.
*Corresponding author: cahayani.wa@ub.ac.id
INTRODUCTION. The adverse effects of antipsychotic agents and recurrent episodes remain considerable
challenges in schizophrenia management that drive the patient to seek medicinal plants as alternative medicine.
However, efficacy and safety aspects of herbal medicine in schizophrenia have not been fully explored. Thus,
this study was purposed to investigate the potential candidate of plant metabolite compound either as therapy
or adjuvant for schizophrenia. METHODS. In silico study was performed by molecular docking using seven
metabolite compounds of Indonesian herbal medicine towards 5-HT2a (PDB IB: 7wc8) protein. The ligands
of curcumin, asiaticoside (madecassol), ginkgolide B, bacoside A, mitragynine, 7-hydroxymitragynine, and α-
asarone were retrieved from PubChem. For virtual docking, AutoDock Vina in PyRx software was used. 5-
HT2a and ligand binding visualized and analyzed using PyMol and Discovery Studio Visualizer. RESULTS
AND DISCUSSION. Curcumin has the highest negative binding affinity (-9.3 kcal/mol) among metabolite
compounds, surpassing clozapine, lumateperone, lysergic acid diethylamide (LSD), and others. Two amino
acid residues that show the identical interaction between 5-HT2a-curcumin and 5-HT2a-lumateperone were
Leu229 and Val366. Val 156 amino acid residue mediated the interaction between 5-HT2a-curcumin and 5-
HT2a-clozapine. However, the interactions between 5-HT2a-curcumin and 5-HT2a-LSD were identical at four
amino acid residues: Val156, Phe340, Leu229, and Trp151. CONCLUSION. The 5-HT2a receptors may
respond to curcumin as an agonist or antagonist. The mechanism of action of these active drugs on 5-HT2a
receptors, which may help in schizophrenia treatment, requires further investigation.
REFERENCE
[1] Hoenders HJR, Bartels-Velthuis AA, Vollbehr NK, et al. J Nerv Ment Dis. 2, 81-101 (2018).
[2] Schmidt CJ, Sorensen SM, Kehne JH, et al. Life Sci. 56, 2209-2222 (1995).
[3] Miodownik C, Lerner V, Kudkaeva N, et al. Clin Neuropharmacol. 42, 117-122 (2019).
Oral Presentation
The Addition of Red Dragon Fruit Peel and Lemon Peel in

The Development of Coconut Water Fermented Beverage
Products using Lactic Acid Bacteria to Decrease Tannin
Levels and Increase Antioxidant Activity
Sofi Nabila, Arie Srihardyastutie*, Sasangka Prasetyawan, Aulanni’am, and Rurini Retnowati
Chemistry Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, 65145, Indonesia
(email: Sofinabila@student.ub.ac.id , sasangka@ub.ac.id, rretnowati@ub.ac.id, aulani@ub.ac.id )
*Corresponding author: (arie_@ub.ac.id)
INTRODUCTION. Coconut water is part of the coconut fruit which has a fairly complete nutritional content
with very limited added value. Bioactive compounds contained in coconut water are thought to have high
antioxidant activity, due to the presence of tannin compounds. However, this compound is anti-nutritional
because it can interfere with the availability of protein in the body. Considering the risk of tannins, these
components can be reduced by fermentation techniques using lactic acid bacteria, where the tanase activity of
lactic acid bacteria is able to degrade tannins into glucose and gallic acid. The aims of this research is to study
the addition of dragon fruit peel and lemon peel in fermentation of coconut water using lactic acid bacteria in
reducing tannin levels and its potential capability as antioxidants. METHODS. Coconut water fermented drink
is made by fermentation technique using lactic acid bacteria with the addition of variations in mass of dragon
fruit peel and lemon peel consisting of 5 treatments, namely P1 = without the addition of fruit peel, P2 = 30 g
of red dragon fruit peel, P3 = 30 g of orange peel , P4 = 20 g red dragon fruit peel : 10 g lemon fruit peel, P5
= 10 g red dragon fruit peel : 20 g lemon peel. The optimum conditions for fermenting coconut water using
lactic acid bacteria were determined from the decrease in tannin levels (mg/100mL), total acid (%) and the
highest total lactic acid bacteria (cfu/mL). The optimum conditions of fermentation were then tested using an
HPLC instrument. Finally, the antioxidant activity was tested using the FRAP (Ferric Reducing Antioxidant
Power) method. RESULTS AND DISCUSSION. The results showed that the addition of dragon fruit peel
and lemon peel in coconut water fermented drinks using lactic acid bacteria can reduce tannin levels and can
increase total acid levels and the number of lactic acid bacteria formed in coconut water fermented drinks. The
best proportion was obtained in the treatment of 10 g of dragon fruit peel: 20 g of lemon peel with the highest
decrease in tannin content was 83.94 mg/100mL, with a total acid content of 1.13% and a total lactic acid
bacteria of 8.4 × 107 cfu/mL. The HPLC chromatogram showed that fermented coconut water using lactic acid
bacteria contained organic acid compounds such as lactic acid, acetic acid, and ascorbic acid. The highest
antioxidant activity test was 82.37 mmgAAE/g. CONCLUSION. This study confirmed that the addition of
dragon fruit peel and lemon peel affected the degradation of tannins and increased antioxidant activity during
coconut water fermentation using lactic acid bacteria.
REFERENCE
[1] T.D. Ananda, A. Srihardyastutie, S. Prasetyawan, and A. Safitri, "Effect of mixed inoculums volume and ph on anti nutritional
level in cabbage fermentation using saccharomyces cerevisiae and lactobacillus plantarum", IOP Conference Series: EMaterial
Science and Engineering, vol. 546, (2019).
[2] L. C. Zhou, D.N. J, A.S.K. Yong, R. Shapawi, and Y.H. Lin, “Effects of fermented lemon peel supplementation in diet on
growth, immune responses, and intestinal morphology of asian sea bass, lates calcarifer,” Aquac. Reports, vol.21, pp. 100-801 (2021)
[3] A. Izquiredo-Llopart, A. Carretero, and J. Saurina, “Organic acid profiling by liquid chromatography for the characterization of
base vines and sparkling wines,” Food Anal. Methods, vol. 13, no. 10, pp. 1852-1866 (2022)
[4] Y.S. Zhao, “Fermentation affects the antioxidant activity of plant-based food material through the release and production of
bioactive components” Antioxidants, vol. 10, no. 12, (2021)
Oral Presentation
Design of multifunctional engineered-modular endolysin as a

‘tailor-made’ antimicrobial candidate for Streptococcal and
Staphylococcal infections
Hariyatun Hariyatun1*, Eko Wahyu Putro2, Asep Muhamad Ridwanuloh1, Gita Syahputra3,
and Wien Kusharyoto1
1
Research Center for Genetic Engineering, National Research and Innovation Agency, Jl. Raya Bogor Km. 46,
Cibinong, Bogor, West Java, Indonesia. E-mail: hariyatun@brin.go.id, asep.muhamad.ridwanuloh@brin.go.id,
wien.kusharyoto@brin.go.id
2
Research Center for Applied Microbiology, National Research and Innovation Agency, Jl. Raya Bogor Km. 46,
Cibinong, Bogor, West Java, Indonesia. E-mail: eko.wahyu.putro@brin.go.id
3
Research Center for Vaccine and Drugs, National Research and Innovation Agency, Jl. Raya Bogor Km. 46, Cibinong,
Bogor, West Java, Indonesia. E-mail: gita.syahputra@brin.go.id
*Corresponding author: hariyatun@brin.go.id
Streptococcal and Staphylococcal infections can cause diverse animal and human diseases and further systemic
infections which could lead to serious zoonotic or foodborne diseases. Increasing antibiotic resistance of these
Gram-positive pathogenic bacteria poses a major threat to public health. Bacteriophage modular endolysins,
and their engineered ones, are promising alternative antimicrobial candidates which are unlikely to evoke
antimicrobial resistance. Given the previous success and the potential of its modular design, therefore, this
research developed the design of multifunctional engineered-modular endolysin as a ‘tailor-made’
antimicrobial candidate targeting both Streptococcal and Staphylococcal infections. Accordingly, this research
in silico-designed the synthetic gene encoding the multifunctional engineered-modular endolysin and
investigated the protein expression using a rhamnose-based tightly regulated expression system, as well as its
purification and characterization. The multifunctional engineered-modular endolysin expression cassette
encoding the fusion of three different domains of modular endolysins, i.e. an enzymatically active domain
(EAD) of the λSA2 modular endolysin recombined with a cell wall-binding domain (CBD) and an EAD of the
phiZJ20091101-3 modular endolysin, has been successfully designed. This expression cassette design could
facilitate the generation of other improved engineered-modular endolysins. The multifunctional engineered-
modular endolysin designed here was in silico predicted to have higher binding activity and relatively
maintained catalytic activities than that of its respective templates. In addition, based on online allergenicity
analysis, it has no possibility to elicit allergenic reactions, thus it is considered as safe for medical applications.
Furthermore, the multifunctional engineered-modular endolysin designed has been successfully expressed in
Escherichia coli NiCo21(DE3) as an insoluble protein. The protein has also been coupled purified and on
column-refolded and characterized to have a molecular weight of approximately 48.2 kDa. These results
suggest that the multifunctional engineered-modular endolysin designed here could be potential as a ‘tailor-
made’ antimicrobial candidate for Streptococcal and Staphylococcal infections and may offer further
generation of other improved engineered-modular endolysins.
Oral Presentation
Effect of Water Clover (Marsilea crenata Presl.) extract on

SOD1 and Nrf2 Expression Level of Male Rat (Rattus
norvegicus) Testis
Sri Rahayu1, Sasangka Prasetyawan2, Sri Widyarti3
1
Biology Department, Faculty of Natural Sciences, Brawijaya University, Malang 65145,email : srahayu@ub.ac.id
2
Chemistry Department, Faculty of Natural Sciences, Brawijaya University, Malang 65145,email : sasangka@ub.ac.id
3
Biology Department, Faculty of Natural Sciences, Brawijaya University, Malang 65145,email : swid@ub.ac.id
*Corresponding author: srahayu@ub.ac.id
INTRODUCTION. The testes are the primary male reproductive organs for spermatogenesis and
steroidogenesis. During spermatogenesis and steroidogenesis a number of ROS (Reactive Oxygen Species)
are produced as a result of metabolism. Capacitance, hyperactivation, motility, and binding of spermatozoa
with oocytes are some of the cellular processes that require sufficient amounts of ROS. High levels of ROS
can cause oxidative stress (SO), which has an impact on DNA fragmentation and decreased sperm membrane
integrity. This will result in a decrease in sperm quality. For this reason, the amount of ROS in the spermatozoa
environment needs to be maintained, one of which is by increasing antioxidants. Naturally, the body can
synthesize endogenous antioxidants, including Superoxide dismutase (SOD), catalase (CAT) and glutathione
peroxidase (GPx), whose expression is regulated by nuclear factor erythroid 2–related factor 2 (Nrf2). The
activity of nrf2 can be induced by the presence of ROS or some chemical compounds belonging to the
flavonoid group. Water clover (Marsilea crenata) is a plant that contains various compounds that act as
antioxidants, including quercetin, gallic acid, β-carotene, genistein, apigenin, daidzein, naringenin, β-sitosterol
and formonetin. The results of the in silico study showed the potential of several active compounds of M.
crenata in activating the synthesis of endogenous antioxidants through the Nrf2/Keap1 pathway, so it was
assumed that in vivo M. crenata could increase the synthesis of endogenous antioxidants in the testes.
METHODS. This study used 24 male rats (n=6), which divided into 4 groups: control (normal rat without any
treatment); PS1, PS2 and PS3 (normal rat which orally received ethanol extract of M. crenata leaves at a dose
of 43.2, 86.4 and 129.6 mg/200 g BW for 30 days, respectively). The expression level of Nrf2 and SOD1 were
analyzed using flow cytometry. RESULTS AND DISCUSSION. The result showed that extract ethanol of
water clover significantly affected the expression of SOD1 and Nrf2 in all groups. The administration of
ethanol extract of water clover dosage 2 (0.432 mg/gBB) was significantly increased the expression of SOD1
and dosage 1 (0,216 mg/gBB) was increased the expression of Nrf2 testicular cells. CONCLUSION. M.
crenata can increase the expression of antioxidant SOD1 and transcription factor nrf-2
REFERENCE
[1] Ordobez A., Bellido R., Vassena R., Barragan M., and Zambelli F.Biology. 9 (269) : 1-22 (2020)
[2] Ko, E.Y., Sabanegh, E.S., Agarwal, A. Fertil. Steril. 102, 1518–1527 (2014).
[3] Wright, C., Milne, S., Leeson, H. Reprod. Biomed. Online 28, 684–703 (2014).
[4] Fraczek, M., Lewandowska, A., Budzinska, M., Kamieniczna, M., Wojnar, L., Gill, K., Piasecka, M., Kups, M., Havrylyuk, A.,
Chopyak, V., Nakonechnyy, J., Nakonechnyy, A., Kurpisz, M. Int. J. Environ. Res. Public. Health 19, 2713 (2022)
[5] Kumar, N., Singh, A.K. Environ. Sci. Eur. 34, 6 (2022)
[6] Yu, B. dan Z. Huang. 2015.. BioMed Reseacrh International (2015).
[7] Yao, Q.M., Wang B., An X., Zhang J., Ding L. Endocrine connection. 7 (1) : 220-231(2018)

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The 4th International Seminar on Smart Molecule of Natural Resources (ISSMART) – Asian

Federation of Biotechnology (AFOB) 2022 | i

Assalamualaikum wr. wb.

On behalf of organizing committee of ISSMART 2022, I would like to extend my warmest

Wassalamulaikum wr. wb.

Rector of Universitas Brawijaya

I would also like to thank to our keynote speakers:

• Prof. Katsuhiro Mijayija from University of Agriculture, Japan.

Please enjoy ISSMART – AFOB 2022.

Malang, 23 August 2022

Chairman of the Organizing Committee

Dr. dr. Nia Kurnianingsih, M.Biomed

Link Zoom Meeting for Plenary Session (Main Room)

Moderator: Tomohiko Sasase, Ph.D

09.15 – 09.45 WIB : Keynote speaker speech

09.45 – 10.15 WIB : Keynote speaker speech

10.15 – 10.30 WIB : Discussion

Moderator : Regina Putri Virgirinia, Ph.D

08.30 – 09.00 WIB : Keynote speaker speech

09.00 – 09.30 WIB : Keynote speaker speech

09.30 – 09.45 WIB : Discussion

12.30 - 12.40 Daniel Tumbur Hamonangan Sirait IN SILICO STUDY OF BIOACTIVE

10.10 - 10.20 Novita Titis Harbiyanti (Faculty of SLEEP DISTURBANCES SCREENING

Application of urinary liver-type fatty acid-binding protein

Exploring Bio-Inspired Green Metal Nanoparticles Synthesis

Overview Exploration of Natural Bioactive Compounds as

Infectious diseases transmitted between animals and humans

Key words: Zoonoses, Corona, virus, public health.

Molecular Docking Analysis of Centella asiatica’s compounds

Discovery of Specific Recombinant Antibody for Smart

Regulatory mechanism of morphogen signals

Cell-based evidence to the Anti-COVID-19 potential of

The Effect of Serial Dilution on Viability 3T3-L1 Cell Line

3T3-L1 cell line

Color Decoration of Chicken Egg Using Natural Dye Molecule

IN SILICO STUDY OF BIOACTIVE COMPOUNDS FROM

The Effect of Black Cumin Extract in Self Nanoemulsifying

The Potential of Ferulic Acid as an Anti-virulence to Combat

*Corresponding author: fatchiya@ub.ac.id

INTRODUCTION. Pathogenic bacteria contain virulence factors, including lipoproteins (LPP),

In Silico and ADMET Analysis of Potential Compounds in

*Corresponding author: Fatchiyah Fatchiyah e-mail: fatchiya@ub.ac.id

Comparison of The Effect Between Black Cumin (Nigella

Computational Insight on Potential Activity of Star Apple

Virtual Based Screening of Rosmarininc Acid and its

The Potential of Bioactive Compounds from Cyperus rotundus

Difference Effect Between Black Cumin Seeds Extract (Nigella

INTRODUCTION. As hepatoprotector and antidiabetic1, Nigella sativa is acknowledged to prevent organ

Average score in each group

In Silico Approach of Potential Compounds in Tomato

Effect of Black Cumin Seed Extract in Self Nanoemulsifying

Effect of Black Cumin Extract in Self Nanoemulsifying Drug

Talenta Nugroho Suryanto Mahardhika1*, Syaefudin Ali Akhmad2

Pathophysiological Analysis of kidney and comparison of male

*Corresponding author: (wkana0327@outlook.jp)

Investigation of the usefulness of hL-FABP tg mice as

Marika TOHMA1*, Keiichi Ohata2,3, Ayaka HORIUCHI2 Teppei Uechi1, Noriko

Anti-angiogenic effect of non-contact electro capacitive cancer

Keywords: angiogenesis, breast tumor, ECCT exposure, HIF1α, VEGFA

Enzymatic Synthesis and Cytotoxicity Activity of Phenolic

*Corresponding author: cairns@sut.ac.th

Keywords: Os9BGlu31, transglycosylation, β-glucogallin, cytotoxicity, anti-cancer.

BRAZILIN AND 3 – O- METHYL BRAZILIN REVEALED

*Corresponding author: dewiratihtirtosari@ibrahimy.ac.id