Virtual Laboratory Activity - BIOL 1121 - Unit 8

Data Collection and Result

While you are working on this virtual lab activity, please record your data in the tables
below.
1) Data Collection Tables

 Section 1: Expression
Gene 1
Table 1. Expression Gene 1A

Set 1 Results Inference

Positive Transcription Factor Gene transcription occurred Positive Transcription Factor

Polymerase Successfully on a boosts gene transcription
transcribed
and turn a gene On.
region and mRNA
produced.

Table 2. Expression Gene 1B

Set 2 Results Inference

Negative Transcription Gene transcription was Negative Transcription

Factor unsuccessful. factor represses
Polymerase transcription and turn gene
off.

Table 3. Expression Gene 1C

Set 3 Results Inference

Positive Transcription Factor By attaching ribosome there Ribosome catalyzes the

 Polymerase was synthesis in the gene Formation of protein.
Ribosome expression
by translating
genetic code transcribed in
mRNA into protein.

Gene 2
Table 4. Expression Gene 2

Set 1 Observations Inference

Positive Transcription By adding mRNA destroyer mRNA destroyer silences or

Factors (Type 1 and Type 2)
it signals to proteins that turns gene off before
Polymerase translation OCcurs
they destroy mRNA before
Ribosome as controlling measure
translated to make protein.
production process of
mRNA Destroyer
protein.

Gene 3
Table 5. Expression Gene 3A

Set 1 Results Inference

Positive Transcription Factor Gene transcription was Positive Transcription Factor

(Type 1) unsuccessful.
ffType 1) interacts with
Polymerase
specific polymers but not all
Ribosome
of them.

Table 6. Expression Gene 3B

Set 2 Results Inference

Positive Transcription Factor Gene transcription occurred A combination of type 1 and

 (Type 1 and Type 2) successfully on a transcribed positive transcription
Polymerase region and mRNA activates gene to prduce
Ribosome is 2
produced. mRNA.

 Section 1: mRNA
Table 7. mRNA 1

Set 1 Results Inference

Positive Transcription Factor Gene transcription was Transcription factor proteins

(middle concentration and unsuccessful.
regulate genes by not
low affinity)
binding on low affinity
RNA Polymerase (low
affinity) binding sites.

Table 8. mRNA 2

Set 2 Results Inference

Positive Transcription Factor Gene transcription occurre- Transcription factor proteins

(high concentration and
successfully on a transcribe regulate genes by binding
high affinity)
region and on high affinity binding
RNA Polymerase (high
affinity) mRNA is sites.
produced.
 Section 3: Multiple Cells
Table 9. Multiple cells 1

Set 1 Observations Inference

Positive transcription The When

factors - High concentration.
mRNA destroyer – Low
concentration
Positive transcription
factors - High affinities
Polymerase – High affinity
Protein Degradation – Fast
results
are
Escherichia coli cells
expressing green florescent
proteinffGFP). The cells are
genetically identical, the
shows
expressing differing
amounts of protein, some
average and some low.
there is
destroyer low
mRNA
concentration, it allows an
average production or
translation of protein.

Table 10. Multiple cells 2

Set 2 Observations Inference

Positive transcription The When there is high

 factors - High concentration. Results shows Escherichia concentration of mRNA
coli cells expressing no
mRNA destroyer – High
presents protein.
concentration
destroyer,
Positive transcription
protein
factors – High affinities
production is inhibited or
Polymerase – High affinity
restrained.
Protein Degradation – Slow

2) Conclusion
Write a conclusion about this virtual lab activity in 5-6 sentences.
. DNA is divided into genes that can express polypeptides or functional RNA. Gene expression
is a process in which information from genes is used to produce material. Genes encoding po
lypeptides are expressed in two steps: transcription and translation. Transcription involves c
opying a strand of DNA into RNA, which undergoes further processing to become messenge
r RNA ffmRNA). Translation, on the other hand, requires tRNA to determine the nucleotide se
quence of the mRNA to specify the amino acid sequence of the polypeptide. The presents of
mRNA destroyer is crucial for proper gene expression and regulating the levels of proteins
within cells.