LIFS2720– Introductory Biochemical Laboratory

Worksheet 08

Metabolic Syndrome - Lipids

Name:Tang Tsun Long

ID:20867909

Group number:23

Q1. Construct standard curves to calculate the following:

(6 marks)
a. Total cholesterol in plasma for control and fructose-treated rats

Chlesterol concentration v.s.Absorbance

0.7000
y = 0.0294x + 0.0624
0.6000 R² = 0.9984
Absorbance

0.5000

0.4000

0.3000

0.2000

0.1000

0.0000
0.0000 5.0000 10.0000 15.0000 20.0000 25.0000

Chlesterol Concentration
y = 0.0294x + 0.0624

For control, absorbance is 0.1445

0.1445=0.0294x + 0.0624
x=2.7925 mg/dL

For fructose-treated rats, absorbance is 0.2472

0.2472=0.0294x + 0.0624
x=6.2857mg/dL
b. Triglycerides in plasma for control and fructose-treated rats

Triglyceride concentration vs
Absorbance
0.8000
0.7000
Absorbance
0.6000
0.5000 y = 0.0319x + 0.1076
0.4000 R² = 0.998
0.3000
0.2000
0.1000
0.0000
0.0000 5.0000 10.0000 15.0000 20.0000 25.0000
Triglyceride concentration

y = 0.0319x + 0.1076

For control, absorbance is 0.2340

0.2340 = 0.0319x + 0.1076

x=3.9624 mg/dL

For fructose-treated rats, absorbance is 0.3959

0.3959 = 0.0319x + 0.1076

x=9.0376 mg/dL

c. LDL in plasma for control and fructose-treated rats

LDL Cholesterol concentration v.s.

Absorbance difference between 600nm and
700nm
0.5000
Difference

0.4000

0.3000
y = 0.0224x + 0.0197
0.2000 R² = 0.9299

0.1000

0.0000
0.0000 5.0000 10.0000 15.0000 20.0000 25.0000

LDL Cholesterol concentration

y = 0.0224x + 0.0197

For control, absorbance is 0.0257

0.0257= 0.0224x + 0.0197

x=0.26786 mg/dL

For fructose-treated rats, absorbance is 0.0936

0.0936= 0.0224x + 0.0197

x=3.2991 mg/dL

d. HDL in plasma for control and fructose-treated rats

HDL Cholesterol concentration v.s.

Absorbance difference between 600nm and
700nm
0.3500
Difference

0.3000
0.2500 y = 0.014x + 0.0004
R² = 0.9819
0.2000
0.1500
0.1000
0.0500
0.0000
0.0000 5.0000 10.0000 15.0000 20.0000 25.0000

HDL Cholesterol concentration

y = 0.014x + 0.0004

For control, absorbance is 0.1068

0.1068 = 0.014x + 0.0004

x=7.6 mg/dL

For fructose-treated rats, absorbance is 0.241

0.241= 0.014x + 0.0004

x=17.186 mg/dL
e. Hepatic cholesterol for control and fructose-treated rats
Hepatic cholesterol vs Absorbance
0.8000
0.7000
y = 0.0308x + 0.004
Absorbance 0.6000 R² = 0.9063
0.5000
0.4000
0.3000
0.2000
0.1000
0.0000
0.0000 5.0000 10.0000 15.0000 20.0000 25.0000
Hepatic cholesterol

y = 0.0308x + 0.004

For control, absorbance is 0.1135

0.1135= 0.0308x + 0.004

x=3.5552 mg/dL

For fructose-treated rats, absorbance is 0.2843

0.2843= 0.0308x + 0.004

x=9.1006 mg/dL

f. Hepatic triglycerides for control and fructose-treated rats

Hepatic triglycerides vs Absorbance
0.8000
0.7000 y = 0.0305x + 0.0065
Absorbance

0.6000 R² = 0.9087
0.5000
0.4000
0.3000
0.2000
0.1000
0.0000
0.0000 5.0000 10.0000 15.0000 20.0000 25.0000

Hepatic triglycerides Concentration

 y = 0.0305x + 0.0065

For control, absorbance is 0.1561

0.1561 = 0.0305x + 0.0065

x=4.9049 mg/dL

For fructose-treated rats, absorbance is 0.2749

0.2749 = 0.0305x + 0.0065

x=8.8 mg/dL

Q2. Compare, describe and explain differences between control and fructose-treated
calculations for each of the above.
(3 marks)

First ,there are significant difference in the concentration of cholesterol and

triglycerides in plasma between control and fructose-treated rats. This is because for
the fructose-treated rats, they will accumulate excessive carbohydrate and produce
more triglycerides and cholesterol in the body than control rats.

Second,for the LDL and HDL concentration in plasma,in fructose-treated rats, both
concentration are generally higher than control rats. And in control rats, the LDL
concentration are realaively low and HDL concentration is relatively high, vice versa
for fructose-treated rats. The reasons of these observation is because fructose-treated
rats accumulate excessive carbohydrate, and the liver cannot function well to convert
LDL into HDL. Thus, more LDL will accumulate in the plasma while a higher
concentration of HDL is stored in the cells when compare to control rats.

Third,for the concentration of hepatic triglycerides and hepatic cholesterol, both

concentration are generally higher than control rats. The reasons of these observation is
because fructose-treated rats accumulate excessive carbohydrate, and the liver cannot
function well to convert triglycerides and cholesterol into VLDL and LDL.Thus, more
triglycerides and cholesterol will accumulate in the liver.

Q3. Explain why lipids are generally increased in fructose-treated rats as compared
to control.
(1 mark)

This is because for the fructose-treated rats, they will accumulate excessive
carbohydrate, with more calories gained, the body can convert it to triglycerides, and
they will be stored in the cells. Thus, lipids are generally increased in fructose-treated
rats as compared to control rats.