Professional Documents
Culture Documents
DNA Extraction From Fungi, Yeast, and Bacteria
DNA Extraction From Fungi, Yeast, and Bacteria
DNA Extraction From Fungi, Yeast, and Bacteria
1. Introduction Although individual microorganisms may well require a unique DNA extraction procedure, here we include robust techniques for the preparation of DNA from fungi, yeast, and bacteria, which yield DNA suitable for a PCR template. 2. Materials 2.1. Fungal Extraction
1. CTAB extraction buffer: 0.1 M Tris-HCl, pH 7.5, 1% CTAB (mixed hexadecyl trimethyl ammonium bromide), 0.7 M NaCl, 10 mM EDTA, 1% 2-mercaptoethanol. Add proteinase K to a final concentration of 0.3 mg/mL prior to use. 2. Chloroformisoamyl alcohol (241).