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MCB101 Exercise 5
MCB101 Exercise 5
BACTERIA
(bacterium)
= relatively simple (Prok.) single-celled; unicellular organisms defined as mcgs considerably smaller than molds (fungi) Antoni van Leeuwenhoek (1676) -first person to see bacteria while studying (pepper) water infusion -reffered to them as wee animalcules
Cultural Characteristics
PROCEDURE.a
Pseudomonas aeruginosa Enterobacter sakazakii Micrococcus luteus
2/4/2012
PROCEDURE.A
NA slant
PROCEDURE.B
TZCA
unknown
YDCA KMBA NA
PLATES
PLATES
punctiform granular / spindle circular rhizoid
filamentous
irregular
2/4/2012
PLATES
PLATES
before
after
Figure 4. The relative growth requirements for oxygen. The medium without bacteria is yellow, while bacterial growth is indicated by the green color. For example, obligate aerobes and anaerobes, grow respectively only at the top (A) and bottom (D) of a tube of liquid medium. Obligate aerobes often form a film (scum) or pellicle, that floats on the top of the liquid. Facultative aerobes have the best of both worlds, as they are able to grow under both aerobic and anaerobic conditions (C). Microaerophilic bacteria require a little bit of oxygen, but too much is toxic (B).
2/4/2012
Pellicle, turbidity, sedimentation Environmental factors that might alter formation of a pellicle: amount of oxygen enzymes
- cells present, light is scattered - cell mass is proportional to cell numbe *motile baceria - also cause turbidity (movement)
*obligate anaerobes - grow away from the surface where O2 cannot penetrate
KMBA
KMBA
Pure culture of P.fluorescens on Kings B medium under normal light B: Pure culture of P.fluorescens on Kings B medium under U.V light
2/4/2012
KMBA
Pseudomonas aeruginosa
FORM MARGIN ELEVATION Turbidity Sedimentation Pellicle/Ring irregular entire umbonate ++ -
TZCA
Enterobacter sakazakii
FORM punctiform MARGIN entire ELEVATION convex Turbidity Sedimentation Pellicle/Ring +
-
YDCA
Micrococcus luteus
FORM MARGIN ELEVATION Turbidity Sedimentation Pellicle/Ring circular entire convex +; uniform -