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Laemmli Buffer
Laemmli Buffer
http://www.molecularstation.com/wiki/Laemmli_buffer
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Laemmli buffer
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Contents
1 History of the Laemmli buffer 2 Laemmli buffer Background 3 Purpose of the Laemmli buffer 4 Laemmli Buffer Recipe Preparation 5 Recommended Storage Temperature of Laemmli buffer 6 Laemmli buffer References
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02.01.2012 16:14
Laemmli buffer
http://www.molecularstation.com/wiki/Laemmli_buffer
20% glycerol 10% 2-mercaptoethanol 0.004% bromphenol blue 0.125 M Tris HCl The solution has a pH of approximately 6.8. Other formulations have been devised including: Sample Buffer (4X Laemmli Buffer) 2.4 ml 1 M Tris pH 6.8 (Same as upper gel buffer) 0.8 g SDS stock 4 ml 100% glycerol 0.01% bromophenol blue. Final Concentration is .02% 1 ml -mercaptoethanol (electrophoresis grade) 2.8 ml water
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Buffer PH 4
5X TBE
10X 5X
I O Buffer
Laemmli buffer
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02.01.2012 16:14