RIPROSAT ECUADOR First detection of the very virulent form of Infectious Bursal Disease Virus (vvIBDV) in Peru

Marco Cisneros T. DMVZ MSc.

INTRODUCTION
IBDV is widely present in Peru, classical and variant (both A and E) strains are well recognized.
(Icochea 2001, Banda 2006, Ceva findings until 2006)

Since 2004 clinical cases of Bursal Disease were reported in Puerto Maldonado region (south east and far away from poultry producer areas). In August 2007 the region was visited and bursa samples were collected High mortalities between 28 35 d.o.
Layer pullets up to 60% Broilers up to 30%

Samples were taken from three different farms

19 d.o. layers pullets apparently normal 35 39 d.o. broilers with history of clinical IBD at 28 days 27 31 d.o. broilers showing clinical signs of the disease.
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VENEZUELA

COLOMBIA

PERU

BRAZIL

Puerto Maldonado

BOLIVIA

Commercial Pullets (19 d.o.)

No clinical signs or lesions indicative of IBDV infection

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Broilers (35 39 d)

Whitish diarrhea (the only clinical sign) Muscular hemorrhages (most frequent lesion) Some lesions in kidneys and proventriculus No macroscopic lesions in bursas (the bursa shown in the picture is not normal, it is edematous!

Broilers (2731 d)

Depressed birds, ruffled feathers, prostration, fallen wings, whitish diarrhea Mortality rising dramatically
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 Swollen and edematous bursas Extensive hemorrhagic areas in bursas and alterations in folds
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 Muscular hemorrhages (more frequently observed lesion) Proventriculitis, spleen lesions

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DIAGNOSIS
Bursa samples tested at three different labs for molecular detection and characterisation of IBDV.
CEVA Phylaxia : Budapest Hungary (frozen bursas)
RT PCR - Sequencing

SIMBIOS Biotechnology : Canoas, RS Brazil (FTA)

RT PCR - RFLP

Sao Paulo University : Sao Paulo Brazil (FTA)

RT PCR - Sequencing
UNIVERSIDADE DE SO PAULO Faculdade de Medicina Veterinria e Zootecnia

PHYLAXIA R&D Virology

Departamento de Patologia Laboratrio de Ornitopatologia

RESULTS
FARMS Layer Pullets LABORATORY Phylaxia USP Simbios Phylaxia USP Simbios Phylaxia USP Simbios RESULT --Negative --vvIBD vvIBD Group 11 vvIBD vvIBD + Classic Groups 11 + 5 Interpretation

Broilers 35 39 d.o

vvIBD

Broilers 27 31 d.o

vvIBD + Lukert?

Molecular analysis: VP2 hypervariable region

Ceva-Phylaxia: 408 bp (721 1128) Simbios: 248 bp (804 1052) USP: vvIBD 200 bp, Classic 500 bp
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Phylogenetic Tree - Phylaxia

Distance 0.02 DV86/86NL OKYMT/95JP OKYM/91JP VEN7 VEN6 VEN9 CS89/89GB TULA94/RU UK661/86GB D819/3/07PE D819/2/07PE G92/2/03BR BR-7 BR-2 BR-3 G138/3/03BR G138/4/03BR 849VB/87BE

Very virulent IBDVs

52/70GB CEVACIBDL STC/67US 228E U28/88US VarE/85US VarA/85US 3212/88US GLS/87US P2/73DE CEVACGumbo GUMBCT/gen TAD/DE D78 Cu1/76DE PBG98/76GB BURSINE2 00273/73AU

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% Relationship vvIBDVs - Phylaxia

Seq-> OKYMT/95JP UK661/86GB 849VB/87BE Cu1/76DE VEN9 G92/2/03BR G138/4/03B G138/3/03B D819I2PE D819I3PE BR-7

OKYMT/ 95 JP
ID 0.977 0.95 0.924 0.985 0.977 0.963 0.963 0.965 0.965 0.975

UK661/ 86 GB
0.977 ID 0.958 0.926 0.987 0.99 0.975 0.975 0.977 0.977 0.987

849VB/ 87 BE
0.95 0.958 ID 0.924 0.96 0.963 0.948 0.948 0.95 0.95 0.96

Cu1/ 76 DE
0.924 0.926 0.924 ID 0.924 0.931 0.916 0.916 0.928 0.928 0.928

VEN9
0.985 0.987 0.96 0.924 ID 0.987 0.973 0.973 0.975 0.975 0.985

G92/2/ 03 BR
0.977 0.99 0.963 0.931 0.987 ID 0.985 0.985 0.987 0.987 0.997

G138/4 /03 B
0.963 0.975 0.948 0.916 0.973 0.985 ID 0.995 0.973 0.973 0.987

G138/3 /03 B
0.963 0.975 0.948 0.916 0.973 0.985 0.995 ID 0.973 0.973 0.987

D819I2

D819I3

PE
0.965 0.977 0.95 0.928 0.975 0.987 0.973 0.973 ID 1 0.985

PE
0.965 0.977 0.95 0.928 0.975 0.987 0.973 0.973 1 ID 0.985

BR-7
0.975 0.987 0.96 0.928 0.985 0.997 0.987 0.987 0.985 0.985 ID

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ASSIS

RIO ACRE

IAPARI
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Current Situation of vvIBD in Latin America

BRAZIL (1997)
Di Fabio et al, Vet.Rec. 1999, 145(7):203-204

DOMINICAN REPUBLIC (1998)

P.Mant et al, Med.Vet. 2000, 17(1):20-25

VENEZUELA (2001)
Y.Gardin et al. World Poultry. 2003, 19(3):36-38

COLOMBIA (2004)
Ochoa et al. Rev. Plumazos. 2005, 23:10-14

URUGUAY (2004)
M.Hernandez et al, Av.Dis. 2006, 50:624-631

BOLIVIA (2004) ARGENTINA (2006) PERU (2007 2004?) Molecular identification (RFLP)

P.Remorini et al, Av.Dis. 2006, 50:245251

NEXT ?
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Thank you...

marcocisneros@dimune.com

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