INT. J. NEW. INN.

, 2012, 1(1), 18-20

ISSN:2277-4459

EFFECT OF CARBON-DIOXIDE ON PRODUCTION OF ALGAL BIOMASS UNDER DIFFERENT DILUTIONS

Ajay Kumar1, A.K. Giri2, Ashutosh Patel2, Surendra Tripathi2
2

Mewar University, Chittorgarh, Rajasthan IEDS, Bundelkhand University, Jhansi (U.P.)

ABSTRACT
Phosphorus (P) is a key nutrient that stimulates the growth of algae and other photosynthetic microorganisms such as toxic cyanobacteria (blue-green algae), and must be removed from wastewater to avoid eutrophication in aquatic water systems (Oehmen et al 2007). Objective of our work is to study the effect of CO2 on algal biomass production and characterization of wastewater before and after algal biomass production. Wastewater sample was collected. The raw waste and effluents are analysed for pH, dissolved oxygen (DO), Biological oxygen demand (BOD), Chemical oxygen demand (COD), Nitrate (NO3), Phosphate (PO4-) and Ammonia (NH3). After the analysis of wastewater the experiment is setup into the bucket in the open environment. The experiment is setup for 21 days in different dilutions. As per the literature review, Carbon-dioxide helps in increasing the biomass production, so we provide CO2 manually for two hours a day under different photoperiods. Diluted wastewater culture with two times aeration seems to have commanding influence on algal biomass production in prevailing conditions at Jhansi city.

KEYWORDS: Cyanobacteria, Phosphorous and Wastewater.

1. THE PROBLEM
An estimated 700 million Indians have no access to a proper sanitation, and 1000 Indian children die of diarrheal sickness every day. Sewage effluents are largely due to the increasing use of domestic synthetic detergents during the last decade, which has increased the phosphorus content of sewage to 3-4 times the level before the advent of detergents (Stumm and morgan, 1962). The risk of adverse effects to the plant and animal communities in waterways declines as Phosphorous concentrations approach background levels (Mainstone and Parr, 2002). The role of nutrient in water is harmful. In water, the nutrients cause eutrophication and algal bloom. Eutrophication (e.g., over enrichment of nutrients) of marine and surface water caused by human activities and neglect is currently a common problem encountered in many countries (Crump, 1993). The removal of nutrients from wastewater is called wastewater treatment and in this process different types of micro and macrophytes are used. The wastewater treatment is a natural process in pond or lake by the help of microorganisms like bacteria, cyanobacteria, green

algae, fungi and some macrophytes like Eichornia, Salvenia etc.

2. OBJECTIVE OF STUDY
1. To characterize the use of wastewater for algal production. 2. Study the effect of CO2 on algal biomass production. 3. Characterization of wastewater before and after algal biomass production.

3. MATERIALS & METHOD

Wastewater sample was collected. The raw waste and effluents are analysed for pH, dissolved oxygen (DO), Biological oxygen demand (BOD), Chemical oxygen demand (COD), Nitrate (NO3-), Phosphate (PO4-), Ammonia (NH3) as per the standard procedure given in the Standard Method for the Examination of Water and Wastewater published by APHA (1998). Relationship between various physico-chemical parameters were measured by one-way analysis of variance (ANOVA). After the analysis of wastewater the experiment is setup into the bucket in the open International Journal of New Innovations

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