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AS/APR 2011/BMS652

UNIVERSITI TEKNOLOGI MARA FINAL EXAMINATION

COURSE COURSE CODE EXAMINATION TIME

PLANT AND ANIMAL BIOTECHNOLOGY BMS652 APRIL 2011 3 HOURS

INSTRUCTIONS TO CANDIDATES 1. This question paper consists of two (2) parts : PART A (4 Questions) PART B (3 Questions)

2.

Answer ALL questions from PART A and PART B in separate Answer Booklets. Start each answer on a new page. Do not bring any material into the examination room unless permission is given by the invigilator. Please check to make sure that this examination pack consists of: i) ii) the Question Paper two Answer Booklet - provided by the Faculty

3.

4.

DO NOT TURN THIS PAGE UNTIL YOU ARE TOLD TO DO SO

This examination paper consists of 4 printed pages
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AS/APR 2011/BMS652

QUESTION 1 a) Define the following: i) ii) b) Explant culture Callus culture (4 marks) List and explain the steps involved in plant tissue culture techniques. (4 marks) c) Discuss the rationale for the composition of a tissue culture medium. (8 marks)

QUESTION 2 a) Antisense technology is based on stopping the production of a protein. Explain how the antisence technology works. (4 marks) "The Agrobacterium is considered as Natural Genetic engineer of plants". Comment. (4 marks) What is direct gene transfer? What are the methods of direct gene transfer? (5 marks)

b)

c)

QUESTION 3 a) Biotechnology is already contributing enormously to pest management. Discuss some of the benefits and concerns about the use of genetically modified (GM) plants. (8 marks) Discuss the use of biofertizers in agriculture. (8 marks)

b)

QUESTION 4 a) What are molecular markers? (2 marks) b) List four (4) uses of molecular markers in plant and animal breeding. (4marks) c) Briefly explain four (4) specific examples of molecular marker. (8 marks)

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AS/APR 2011/BMS652

PARTB

QUESTION 1 Write short notes on the following: a) Quantitative trait loci. (4 marks) b) Transgenic fish. (4 marks) c) Limitations of animal cloning. (4 marks) d) Endomycorrhizas. (4 marks) e) Patenting of biological materials. (4 marks)

QUESTION 2 Biological control is a population leveling process in which a population of one species lowers that of another. a) List three (3) advantages of using biological control agents in controlling insect pests. (3 marks) The three main types of biological control are conservation, classical biological control and augmentation. Discuss the classical type of biological control and in your answer describe two (2) examples of successful programmes. (7 marks)

b)

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AS/APR 2011/BMS652

QUESTION 3 Traditionally animal models in biomedical research have made used of rats, mice, guinea pigs, hamsters and rabbits. But larger domestic animals are becoming more valid as research models because their anatomy and physiology are more similar to humans. Also, their body and organ sizes are much closer to humans. a) Define the term Biopharming. (2 marks) b) Discuss issues (societal, religious, environmental, safety) involving transgenic livestock in biomedical research. (Hint: transgenic livestock have been used as disease models, as organ and tissue donors, xenogenic cells and tissues, producers of new medications). (8 marks)

END OF QUESTION PAPER

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