1o.

Simpsio Integrado de Cincias da Sade e Biolgicas do Centro Acadmico de Vitria/UFPE Vitria, 08 e 10 de novembro de 2006

"Biosensores: realidades e perspectivas "

Prof Dr. Jos Luiz de Lima Filho LIKA (UFPE)

Laboratrio de Imunopatologia Keizo Asami

Introduction
It is hard to place a price tag either on human life or on the quality of life. This has become starkly evident since the attack on the World Trade Center on September 11, 2001 and Madrid on 11 march, 2004. There is a similar, yet silent, crisis that is dramatically affecting the quality of life for individualslack of access to affordable and highquality healthcare.

Technology is the key to enhancing the quality of life

Consider the following facts:

In a recent report, the Institute of Medicine concluded The U.S. healthcare delivery system does not provide consistent, high-quality medical care to all people Experts estimate that as many as 98,000 people die in any given year from medical errors that occur in hospitals. More people die annually from medication errors than from workplace injuries.

And Then

Healthcare spending in the U.S. soared to US$1.42 trillion in 2001, as across-the-board increases fueled the fastest annual growth in a decade Seven major diseases accounted for 80% of deaths in the United States in 1990: heart disease, cancer, diabetes, arthritis, chronic bronchitis, influenza, and asthma In Brazil 32% of deaths are by heart disease, external causes (15%), cancer (15%) folowing by chronic bronchitis, pneumonia and ashma (11%) and parasitoses and infections (9%). For many of these health conditions, early, systematic intervention would be highly beneficial. With universal access to information, todays healthcare consumer is demanding more options and taking control in determining the course of healthcare.

Responding to the Healthcare Challenge

Reduce healthcare costs while maintaining the high quality of care; provide access to care for as many people as possible; provide easy access to specialized professionals anywhere and at anytime; shift the focus of healthcare expenditures from treatment to prevention through wellness programs; control length of hospital stay and decentralize the provision of healthcare; address the increase in the aging population and caring for chronically ill patients.

Actual procedure - HOME

Complex Hospital

Cost

Transport, Nurse, exam appointment

Actual procedure - hospital

Hospital Local Complex Hospital

Maternidade

Cost
Paciente Removido

Patient exam

Patient is re-exam

Transport, Nurse, exam appointment

New Procedure UFPE

Complex Hospital HC UFPE

Maternidade

Safety
SUCESS Conexo via Internet

LIKA, HC, Informatics - UFPE

Internet System - UFPE

Royal Brompton Londres - GB

UCMF

HOSPITAIS/ POSTOS DE SADE

CISAM

HOSPITAIS/ POSTOS DE SADE

HOSPITAIS/ POSTOS DE SADE

CSSE

Casa de Sadeda Encruzilhada -Caruaru - PE Maternidade Santa Efignia UPE Unidade de Cardiologia & Medicina Fetal

Intranet System - UFPE

Laboratory Doctor

Internet

Biosensors - Glucose -Uric acid -Urea -TGO,TGP

Intranet
Local link

UCL

Patient room

Nurse Monitoring Blood pressure Temperature Liquid Flow Diurese

Services - Alarm - INTERNET - Reports

Retornar

How improve the health quality?

System of information Diagnostics Sensors Temperature, pH, gases, images etc. Biosensors

Global Biosensors Markets

Biosensor market application

Clinical (89%), Industrial (6%), Environmental/waste water (3%) Food & beverage applications (1.5%). by 2005 medical biosensor sales will reach over $8 billion and hold the overwhelming share (96%) of all biosensor sales

Where are you going to use them??

Sport Home Travel

Work

Hospital

Introduo

Biossensores

Dispositivos que constituem um subgrupo de sensores qumicos, onde molculas biolgicas, tais como anticorpos, antgenos, enzimas, receptores, organelas, clulas, cidos nuclicos, lectinas, entre outros, so integrados no processo de reconhecimento qumico. Primeiro biossensor (Clark & Lyons, 1962)

Caractersticas ideais

Sinal de sada proporcional concentrao ou a atividade do analito Rpido tempo de resposta Seletivo Sensvel

Transdutores
0.000

Processamento do sinal

Eletroqumica:

-Amperomtrica

-Condutimtrica -Potenciomtrica

Transduo

Eletromagntica:
-ptica -Acstica

Trmica
Camada ativa (reconhecimento molecular

Interface

Analito

Amostra

Eletrodos impressos (screen printed)

Rod o Tel a Tinta supo rte Impress o manual Eletr odo impr esso

Impresso automtica Imobilizao da

Tintas utilizadas
Prata

biomolcula

Grafite Prata/Cloreto de prata

Carbono

Biosensor commercial

Glucose Biosensor Diabetes application

Glucose Biosensor version 1 Optical

Glucose Biosensor version 2 Electrochemical

Glucose Biosensor Application

LIKAs glucose sensor

glucose biosensor

glucose biosensor

Hardware FPGA Microcontrolador RAM EPROM

Fabricante Xilinx MHS Toshiba AMD

Modelo Srie XC40XX Intel (P-80C32-16) TC551001BPL-70 AM27C512

V 1.1

Glucose Implantable sensor

Optical glucose meter

Microchips

Piezoelectric Biosensor
Chitosan film

Crystal
Electrodo Quartz disk
Gold electrode

Oscilator
Frequency measure Power Suppy

QCM System for Leukemia

0,4 0,3
(F1-F2)/F1 0,2 ssDNA dsDNA Sem DNA Imobilizao Cristal Padro

0,1 0

Frequency measure

Crystal

Expresso gnica e Macro/microarrays

Regina Maki Sasahara, PhD

Product Specialist Genomics GE Healthcare Biosciences

Gene Expression Analysis

What is it ?

Northern Blotting, DNAarrays, RT PCR, reporter gene assay

Western Blotting, imunofluorescence , imunoprecipitation , 2D

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Gene Expression Analysis

- Regulation of gene expression is a fundamental cellular control mechanism that has relevance in development, carcinogenisis, cell cycle control, drug responses, etc;

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Cells and Gene Expression

Red blood cells cell Gene A Gene B Gene C

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Neuron cell

Skin

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Why are DNA arrays Important?

DNA array can be used to examine the expression of hundreds or thousands of genes at once.

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The Microarray Workflow

Probe Preparation & Deposition Target Preparation & Hybridization Scanning & Data Analysis

Array Spotter

ID

Array
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Scanne 38r

Array WoRx microarray scanner (Applied Precision)

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System of information Mobile sensor network architecture

Goals: Development of an architecture for a wireless body area network (BAN) of intelligent sensors. The individual sensors (monitors) will be integrated into a distributed wireless system for monitoring of vital physiological human signals.

Remote sensor

Mobile sensor network architecture

Hospital
sensors

Personal Server (PDA, Cell phome)

Local Server

Hospital information system (HIS) Mobile intelligent network

Wireless communication

Mobile sensor network architecture

Applications (Personal Well Assistant)

Tele-homecare Hospital patient monitoring Real-time personal monitor

Smart shirt sensor

Applications of Smart shirt

IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE MAY/JUNE 2003 47

FUTURE???

Muito Obrigado !

Venha conhecer o Recife !

OBRIGADO!!!!

Laboratrio de Imunopatologia Keizo Asami - LIKA, UFPE Diretoria@lika.ufpe.br, Tel. (0 xx 81) 2126-8484