TAS2012 Proceeding Presentation

Nanobiotechnology Fusion Systems

2013-01-08

5312FG02-2 Usama Imtiaz

Department of Integrative Bioscience and Biomedical Engineering WB Group, Takanishi Laboratory usama@fuji.waseda.jp 1

Contents
1.

2.

MANIPULATING SINGLE PARTICLES USING STANDING SURFACE ACOUSTIC WAVES 3D OMNIDIECTIONAL CONTOLLABLE ELASTIC IPMC TWEEZER WITH SELF-SENSING AND ADJUSTABLE CLAMPING FORCE ABILITIES FOR BIOMEDICAL APPLICATIONS

Paper Introduction
MANIPULATING SINGLE PARTICLES USING STANDING SURFACE ACOUSTIC WAVES
Xiaoyun Ding, Sz-Chin Steven Lin, Sixing Li, Lin Wang, and Tony Jun Huang
The Pennsylvania State University, USA

Introduction

Manipulation of Micro/nano Objects

e.g cells, bacteria, viruses, DNA etc.

Existing Manipulators and drawbacks:

Optical Tweezers

High power laser, Complex, Expensive, Large

Magnetic Tweezers

Targets must be pre-labeled with Magnetic Material

Dependent on polarizability of particles,. Can adversely affect cell physiology

Electrophoresis based Tweezers

Acoustic-based particle manipulation

Advantages:

Non-invasive Work for a wider range of microparticles regardless of their optical/electrical/magnetic properties. Focusing, Separation, Sorting and Patterning have been realized by acoutic methods before. Authors have fabricated acoustic tweezers capable to precisely control single microparticles in twodimensions

Significance of current work:

Design Overview

A PDMS channel is bonded to a Lithium Niobate peizoelectric substrate. Two pairs of chirped Interdigital transducers IDTs are also present on the substrate.

Working

IDT pairs are independently fed RF signals to generate surface acoustic waves (SAWs), which interfere to form standing waves. Acoustic radiation force drives particles to nodes or anti-nodes depending on their elastic properties, density, compressibility etc. Position of nodes depend on the acoustic wavelength xn=n/2

Particle/cell manipulation in 2D

Particle Manipulation

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Physiological Effect on Sample

HeLa cells were exposed to high power standing SAW fields for 6s, 1 min, 10 min. The results indicate that there was no signifant physiological damage induced to the cell

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Multiple particles

Simultaneously large number of particles can be manipulated. Clusters of particles can be arranged on a single node or multiple nodes.

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Conclusion

Small, Portable, inexpensive particlemanipulation system. Can also be used for sorting (if combined with flow-based microfluidics) Can dexterously manipulate single particles Can handle multiple particles simultaneously, grouped or individually. Have no adverse physiological effect on samples

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Second Paper Introduction

3D OMNIDIECTIONAL CONTOLLABLE ELASTIC IPMC TWEEZER WITH SELF-SENSING AND ADJUSTABLE CLAMPING FORCE ABILITIES FOR BIOMEDICAL APPLICATIONS
Guo-Hua Feng* and Jen-Wei Tsai
National Chung Cheng University, Chiayi, Taiwan

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Introduction

3D omnidirectional accessible IPMC elastic tweezers Can move multi-directionally Adjustable clasping force Self-sensing force by feedback current

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Issues

Flexible Ionic polymer metal composites (IPMCs) have great potential as soft robotic actuators. Conventional Preparation:

Ionic polymer formation Compositing Surface electroding

Usually prepared in large scale and simple shaped IPMC Transducers: Planar IPMC structure with Nafion sandwiched between plated metal electrodes. (PolyTetraFluroEthylene and Perfluorinated vinyl ether sulfonate)

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Fabrication

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Electrode plating

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Experimental setup

IPMC Tweezer can be inserted in the fixture Force balance

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Characterization Front-back movement

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Characterization Sideways

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3D IMPC tweezer capabilities

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Tweezer force and current relationship

Current flowing through the transducer can be calibrated to gauge the tweezers dynamic force. Can determine if touching/holding the object and to sense/adjust clamping force

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Conclusion

3D omnidirectional IPMC tweezer is demonstrated IPMC Transducer conmprises:

Motion element Clamping head

Can move multidirectionally Can provide adjustable clasping force Has many applications