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4-Hb. Estimation
4-Hb. Estimation
4-Hb. Estimation
4th Lab.
Estimation of
Haemoglobin
(Hb. Estimation)
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Lab. no. 4: Hb. Estimation
Procedure:
• In the graduated tube of the Sahliś
haemoglobinometer add (N/10 HCl) up to (2 g%)
mark. Clean the ball of the left ring finger with spirit
& let it dry.
• Give a moderately deep prick with a sterilized
lancet, let a large drop of blood collect over the
finger and then suck in the pipette of the
haemometer up to 20 mm3 (= 20 μl ) mark.
• Wipe the tip of the pipette and immediately transfer
the blood into the graduated tube. Mix the blood and
the HCl & let it remain for (5-10) min.
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Lab. no. 4: Hb. Estimation
3.Brownish coloured acid haematin is formed by the
action of HCl on Hb.
4.Dilute it with D.W mixing it with a stirrer till the colour
matches with the standard colour (coloured plates)
of the haemoglobinometer.
5.Near the end point take three readings: when the
colour of the mixture is slightly darker, when the
colour is exactly similar and when the colour is just
slightly fainter.
The mean of the last 3 readings is taken as the true
reading of haemoglobin concentration.
-Note: the reading is taken on ( g % ) scale(=g/dl).
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Lab. no. 4: Hb. Estimation
Normal ranges:
Just after birth (16-22) g %.
adult male (14-18) g %.
adult female (12-15) g %.
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Lab. no. 4: Hb. Estimation
Photometric method:
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