GUIDE Dr.DINESH K.P.B. WORK BY SINJULA.C.S.

SREE NARAYANA GURU COLLEGE COIMBATORE

 Liver is the largest organ in the human body. It is

involved in major metabolisms and excretion. It is necessary to maintain a healthy liver for well being of human. The major functions of liver are carbohydrate, lipid, protein metabolism. Detoxification, storage of vitamins and secretion of bile are other important functions. There are several diseases which will effect the liver. Some of them are fatal. The severe diseases are hepatitis, cirrhosis, alcohol- liver disease. The major causes of these diseases are toxic chemicals, alcohol consumption, infections and autoimmune disorders.

 Liver intoxication has increased due to exposure of

environmental toxins. There are several hepatotoxicants including carbon tetra chloride, nitrosamines, paracetamol, alcohol, thiocetamide . Most of the hepatotoxic chemicals damage the liver cells by inducing lipid peroxidation and other oxidative damages Nanomaterials are nanoscale particles (1-100nm) , recent studies showed that such particles will also cause damages to vital organs.

 Exposure to nanoparticles leads irritation of skin,

eyes, acute and chronic damages to lungs, liver, kidney etc... Several nanomaterials are commercially available now, some of them are indium, aluminium, tin, silver, gold. Constant exposure to such particles leads to toxicity. Several hepatoprotctive drugs are available which are used in treatments (silymarin,Liv 52,Livergen). Researches are still going on to derive more herbal drugs.

 Quinolines are heterocyclic organic compounds which are

colourless and soluble in organic solvent. Sources of quinolines are petroleum, coal processing, wood preservation ,production and use facilities. Many derivatives of quinolines are still under preparation . Some new varieties are pyrimido quinolines, triazapino quinolines, pyrazolo quinolines. Pharmacological applications of the compounds:Antibacterial, antimycobacterial, antifungal, Antioxidant, anticancer and antiinflammatory actions.

 A series of novel pyrimido quinoline derivatives have

prepared. Antioxidant activities of some of them have checked through FRAP, TEAC, super oxide and nitric oxide scavenging methods. The free radical scavenging activity is due to the presence of hydroxyl groups or imines unit in the quinoline.

 The purpose of this study is to detect the

hepatoprotective action of pyrimido quinoline in liver damage induced by indium titonate nanoparticles in animal model. To study the in vivo biochemical and antioxidant actions of pyrimido quinoline.

 Drugs and chemicals

Collected the chemicals from Karpagam university and standard drug sylimarin from medical store near Palakkad.

Selection of animals
Selected 30 male rats for the study. Then the rats were allowed to adapt under standard condition for a week.

Grouping protocol
The rats were divided in to 5 groups of 6.

 Head

Body
Tail Head and body

Body and tail

Not marked.

Each group had rats with these six different marks.

Each group will receive different dose of drugs. group 1:- control (DMSO) group2:-negative control (Indium titonate) group3:-inducer + standard (indium titonate+ sylimarin) group4:-inducer+ quinolines(first concentration)

group5:-inducer + quinolines(second concentration)

 Control 5 ml/kg body wt of rat (5% DMSO) Standard drug sylimarin Compound quinoline (stock 8mg/ml of DMSO) Inducer nanomaterial (50mg/kg body wt of rat)

Providing all the drugs continuously for 7 days.

On 8th day the rats will be sacrificed.

 Biochemical assay
SGOT/AST(serum glutamic oxalic transaminase)
SGPT/ALT (serum glutamate pyruvic transaminase) Alkaline phosphatase Bilirubin

 Antioxidant assay
Super oxide dismutase (SOD) Reduced glutathion (GSH) Catalase Thiobarbituric acid reactive substances Glutathion peroxidase

Histopathological studies
Viewing the difference in the morphology of liver cells, between control, standard and the test.