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Eukaryotic DNA Replication Blok9
Eukaryotic DNA Replication Blok9
Eukaryotic DNA Replication Blok9
DNA Polymerase
involved in initiation synthesizes an RNA primer then adds dNTPs a complex of four subunits 50-kD and 60-kD are primase subunits;180-kD subunit DNA polymerase synthesizes 8-10 nt RNA primers, then adds DNA to the RNA primers low processivity of DNA synthesis (200 nt) has no 3 -5 exonuclease activity (proofreading), yet has high fidelity
DNA Polymerase
the principal DNA polymerase in eukaryotic DNA replication has 3-5 exonuclease activity consists of a 125 kdal and a ~50 kdal subunit the 50 kd subunit interacts with PCNA (Proliferating Cell Nuclear Antigen) is highly processive when in association with PCNA
DNA Polymerase -required for replication, but its role is unclear -may substitute for DNA polymerase in lagging strand synthesis
DNA Polymerase -role in DNA repair (doesnt participate in replication)
PCNA
Pol
RPA
RFC
7. 8.