Plant antibiotic production

through plant tissue culture

Call us cul tures deri ved fr om oni on DUSHYANT KUMAR

BSA-08-613
 Antibiotics

These are the chemical compounds which are

having the property act against the biological
disease producing agents likely bacteria, virus and
fungus.
Example of antibiotics
Antibiotic plant spp.
Quinine cinchona
Barberin Papaver somniferum
Artemisin Artimesia spp.
Ajmalicin Cartharanthus roseus
 Steps of production

Production strategy consist of two distinct phase

Growth phase for cell biomass accumulation
Production phase for biosynthesis and accumulation
of biochemical (antibiotic)
 Nutrient medium

Linsmaier and skoog medium (LS medium).

It contain Basal salt mixture containing micro and
macro elements with vitamins .
All high value biochemicals from cultured plant
cells are secondary metabolities.
Biochemicals production by plant cells is markedly
influenced by the constituent of culture medium &
temp.,light,inoculum size.
Improving bio chemicals production

Suitable culture medium & conditions.

Development of high producing cultures.
Use of elicitors.
Use of organ cultures.
Biochemical content of cell cultures depends on
the plant species the genotype or strain of the
species .
Cell cultures are highly heterogeneous for
biochemical production in that different cells
shows different levels of production .
This variation is used to advantage by screening a
large nu. Of clones for isolation of high producing
clones
Often the high yielding clones so isolated exhibit a
decline in production levels on being maintained by
serial subculture but in several cases stable high
producing clones have been successfully isolated.
For production of biochemicals from plant cell
cultures since it greatly reduces the cost of
production.
 PREPARATION OF PLANT TISSUE
CULTURE MEDIUM

Measure approximately 90% of the required

volume of the de ionized-distilled water in a
flask/container of double the size of the required
volume.
Add the dehydrated medium into the water and
stir to dissolve the medium completely. Gentle
heating of the solution may be required to bring
powder into solution
Add desired heat stable supplements to the medium
solution.
Add additional de ionized-distilled water to the
medium solution to obtain the final required volume.
Set the desired pH with NaOH or HCl.
Dispense the medium into culture vessels
Sterilize the medium by autoclaving at 15 psi (121°C)
for appropriate time period. Higher temperature
may result in poor cell growth.
Add heat labile supplements after autoclaving.