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Determination of Tobacco Specific Haemoglobin Adducts in Smoking Mothers and New Born Babies by Mass Spectrometry
Determination of Tobacco Specific Haemoglobin Adducts in Smoking Mothers and New Born Babies by Mass Spectrometry
Blood samples are centrifuged at 3000 rpm for 10 min to generate packed
RBCs
RBCs were washed thrice with 0.9% saline & lysed by addition of 15 ml ice
cold deionized water with vigorous shaking
Sample preparation for HPB-HB adduct
assay by GC/MS
100l lysed RBC was placed in 7 ml borosilicate glass tube
Analysis of samples
Helium carrier gas was used with flow rate 1.3 ml/min
Injection of sample with split less injection mode with injection port
temperature at 280 C
• Ogawa,M., Oyama, T., Isse, T., Yamaguchi, T., Murakami, T., Endo, Y. and
Kayamoto, T. (2006) Hemoglobin adducts as a marker of exposure to chemical
substances, especially PRTR class I designated chemical substances. J. Occup.
Health., 48(5):314-328
• Schaffler G., Betz C., and Richter E.(1993) Mass spectrometric analysis of tobacco-
specific haemoglobin adducts. Environmental Health Perspectives, 99:187-189
• Shah, T., Sullivan, K. And Carter, J.(2006) Sudden infant death syndrome and
reported maternal smoking during pregnancy. Am. J. Public. Health., 96(10):1757-
1759
• Wagenknecht, L.E., Burke, G.L., Perkins, L.L., Haley, N.J. and Friedman G.D.
(1992) Misclassification of smoking status in the CARDIA study: a comparison of
self-report with serum cotinine levels. Am. J. Public. Health, 82(1):33-36
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