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Bone-06 11
Bone-06 11
Bone-06 11
apatite needles
lie alongside the fibrils.
3. Cells
Osteogenitor
cells
Osteoblasts
Osteocytes
Osteoclasts
A. Osteogenitor cell: stem cell
Location:
In periosteum &
endosteum
L.M.:
Small, spindle shape,
weak basophilic
Function:
Stem cell, differentiate into osteoblast
B. Osteoblast
Location:
located on the
surface of bone
tissue in a layer;
LM:
Pear-shaped,
strong basophilic,
large pale nucleus
EM:
short processes;
rich in rER & Gol.
Function:
osteoblast fibers
g.s osteoid
embedded osteoblasts
Osteoblasts
Function
Ⅰ.Synthesize bone collagen fiber and
ground substance--osteoid
Ⅱ.Release matrix vesicle:
0.1µm in diameter
membrane-coated
function: promote calcification
C. Osteocyte
---structure:
flattened cell with multiple
long thin processes
located in bone lacuna
and bone canaliculus
basophilic cytoplasm
adjacent cells connect in
bone canaliculus by gap
junctions
---function:
Maintain bone matrix
regulate the balance of
calcium and phosphonium
EM: processes
gap junction
between connected
processes
Function:
* intercellular
communication
* molecules are
passed via GJ from
cell to cell.
D. Osteoclast
Location:
located at peripheral
part of bone
LM:
Multinuclear large cell
30-100µm
5-50 nuclei
Acidophilic cytoplasm
Bone matrix
EM:
Mv.(ruffled border)--
processes
clear zone:
under the ruffled
border
microfilament
primary lysosome, Ob.
pinosome and
secondary lysosome
RER, mito. and Golgi
Function:
Clear zone: attached the bone surface to form a
microenvironment
Osteoclasts release lysosomal enzymes
(proteinases, carbonic anhydrase), & acids
(citric acid, lactic acid) to dissolve the bone
matrix
Mv.: increase the absorptive area & absorb
the dissolved products
* belong to the mononuclear phagocyte system
4. Bone architecture
long bone
diaphyses ( )
--the cylindrical part, is
almost totally composed
of compact bone , with a
small component of
spongy bone on its inner
surface around the bone
marrow.
epiphyses ( )
--the bulbous ends, are
composed of sponge
bone covered by a thin
layer of compact bone.
A. Spongy bone
Location: at the end &
inner surface of a
long bone
Structure:
---trabeculae:
formed by parallelly-
arranged lamella
form a spongy-liked
network
---Bone marrow:
hemopoietic tissue
endochondral ossification
Highlights