Other organism

Mycoplasma
 Bacterial without cell wall
 Pleomorphic
 Motile
 Pharynx, oropharynx
 vagina
 Mycoplasma pneumoniae
 Primary atypical pneumoniae
 Walking pneumonia
 Rosette configuration/epithelial cell surrounded by
PMN cell
 Hayflick’s medium
 Mycoplasma hominis
 Oropharynx, genital
 Postpartum sepsis, neonatal abscess
 Fried egg appearance colony
“Fried Egg” Colonies of
Mycoplasmas
 Ureaplasma urealyticum
 Urease
 STD
 Amnionitis, fetal wastage, congenital pneumoniae
 Golden brown colony
 Treatment and prevention
 Treatment
 Tetracycline or erythromycin
 Prevention
 Abstinence or barrier protection
 No vaccine
Rickettsia
 Pleomorphic
 Gm negative
 Obligate intracellular
 Both DNA and RNA are present
 Weil- Felix test- OX-K, OX-2, OX-19
 Typhus fever group
 Rickettsia prowasekii var. prowaseki
 Agent of epidemic typhus
 Body louse
 Agent of Brill-Zinsser’s disease
 Rickettsia prowazekii
 Epidemic typhus
 Brill-Zinsser disease

Fluorescent-Ab staining Vector - Louse

From: G. Wistreich, Microbiology Perspectives, Prentice Hall
 Rickettsia prowasekii var mooseri
 Rickettsial typhi
 Agent of endemic typhus
 Rat flea ( xenopsylla cheopis ) ( rat typhus )

 Rochalimea quintana
 Agent of trench fever
 Body louse
 Shine bone fever/ Quintana fever
Spotted fever group
 Rickettsia rickettsii
 Agent of RMSF
 Tick ( tick-borne typhus )
 Rickettsia conori
 Agent of Mediterranean spotted fever
 Boutonneuse fever ( tick-borne )
 Rickettsia akari
 Rickettsial pox
 mites
 Rickettsia rickettsii
 Rocky Mountain spotted fever

Fluorescent Ab staining Vector - Tick

From: G. Wistreich, Microbiology Perspectives, Prentice Hall
Rash of Rocky Mountain Spotted Fever
Scrub typhus group
 Rickettsia tsutsugamushi
 Agent of scrub typhus
 AKA rickettsia orientalis
 Chigger mites

 Coxiella burnetti
 Q- fever
 Ingestion, inhalation, direct contact
Chlamydiae
 Gram negative
 Intracellular
 Non motile
 With DNA and RNA
 Chlamyia psittaci
 Psitacosis- pneumonia like disease
 Psittacine birds
 Presence of elementary bodies AKA
Lavinthal-Colle- Lille body ( infected cell )
 Chlamydophilia (Chlamydia)
psittaci
 Psittacosis (Parrot fever)
 Ornithosis
 Pathogenesis - C. psittaci
 Inhalation of organisms in bird droppings
 Person to person transmission is rare
 Hematogenous spread to spleen and liver
 Local necrosis of tissue
 Hematogenous spread to lungs and other organs
 Lymphocytic inflammatory response
 Edema, infiltration of macrophages, necrosis and
occasionally hemorrhage
 Mucus plugs may develop in alveoli
 Cyanosis and anoxia
 Epidemiology - C. psittaci

 50 - 100 cases per year in USA

 Organisms present in birds (symptomatic
or asymptomatic)
 Tissue, feces, feathers
 Primarily an occupational disease
 Veterinarians, poultry workers, zoo keepers,
pet shop workers
Treatment and Prevention - C. psittaci
 Tetracycline or erythromycin
 Quarantine of imported birds
 Control of bird infection
 Antibiotic supplementation of food
 Chlamydia trachomatis
 Trachoma- conjunctivitis
 Lymphogranuloma venereum-
characterized by buboes
 Inclusion conjunctivitis
 Unchlorinated swimming pool
 Birth passage
Clinical Syndrome -Trachoma

From: G. Wistreich, Microbiology Perspectives, Prentice Hall

Clinical Syndrome - Lymphogranuloma
Venereum
C. trachomatis (biovar: LGV)
 First stage
 Small painless vesicular lesion at infection site
 Fever, headache and myalgia
 Second stage
 Inflammation of draining lymph nodes
 Fever, headache and myalgia
 Buboes (rupture and drain)
 Proctitis
 Ulcers or Elephantiasis
 Patient with LGV
Bilateral inguinal buboes (arrows)