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Nervous Tissue-Lab
Nervous Tissue-Lab
lab
Nervous system
Central nervous system (CNS)
brain
spinal cord
Nervous tissue
Cellularity :
neurons
supporting cells different in CNS and PNS
Neurons
soma
processes
Dendrites
Axon
Axon hillock
Terminal arborization
Sinaptic buttons
PNS
Neuronal bodies form
nuclei
Axons form nerves
The neuron
Nucleus: centrally , eucromatic with visible
nucleolus
Basophilic granules = Nissl bodies RER
and ribosomes
Only in perykarion
Lipophuscin
Methods of study in OM
H&E staining
Form of perykarion
Little information about axon
Silver staining
good observation of processes
Toluidine blue
highlights the Nissle bodies
www.kumc.edu/instruction/medicine/anatomy/histoweb/nervous/nervous.htm
www.biodidac.bio.uottawa.ca
www.meddean.luc.edu/lumen/MedEd/Histo/frames/h_frame6.html
Types of neurons
Shape of cell body: pyramidal, star-shaped,
granular
Size
large > 100m,
small 10 m
No. of processes
unipolar
bipolar
multipolar
Unipolar neuron
Bipolar neuron
Multipolar neuron
Myelin sheath
synthesized by schwann cell/
oligodendrocyte
lipoproteic structure not obvious on HE
stainig
osmium staining - black
Astrocytes
Satellite Cells
Microglia
Schwann Cells
Ependymal Cells
Oligodendrocytes
oligodendrocytes
Found in both white and gray matter
Form myelin sheath of axons in CNS
Small round, nuclei, with processes
surrounding several axons
Majority of CNS cellularity
Astrocytes
Star-shaped with many processes
Link between neurons and capillaries
Two types:
protoplasmatic: mostly in gray matter,
numerous processes, short and ramified
fibrous: mostly in white matter, fewer
processes and longer
Microglia
In both white and gray
matter
Small, elongated cells
Short, irregular
processes
Dendritic spines
Schwann cells
Form myelin
sheath in PNS
Several
Schwann cells/
1 axon
Satellite cells
In PNS
Small cells
surrounding
neuronal bodies
in ganglia
CONTROL TISSUE :
spinal cord
MAIN
DISTRIBUTION:
astrocytes
STAINING:
Cytoplasm
APPLICATION:
Astrocytic tumors.
Neuropil
It refers mainly to the gray matter between cell bodies.
With conventional stains, this area is not stained
Other preparations demonstrate the numerous neuronal and
glial cell processes that compose the neuropil.
http://courseweb.edteched.uottawa.ca/medicine-histology/English/SS_BasicTissues/ Nervous_Tissue.html