Henno, A., Blacher, S., Lambert, C.

,
Deroanne, C., Noel, A., Lapiere, C., Colige,
A. (2010). Histological and transcriptional
study of angiogenesis and lymphangiogenesis
in uninvolved skin, acute pinpoint lesions and
established psoriasis plaques:
An approach of vascular development
chronology in psoriasis. Journal of
Dermatological Science, 57, 162-169.
doi:10.1016/j.jdermsci.2009.12.006
Anne Bernadette So Kaw

Psoriasis

Lifelong disease
Silvery, flaky areas of dead skin build up on the
Autoiummune disease
surface of the plaques before being shed. The
Plaque psoriasis/ Psoriasis vulgaris skin layer underneath (dermis), which contains the
nerves and blood and lymphatic vessels, becomes
Most common type
red and swollen.
Affects the basal layer of the epidermis (stratum basale/germinativum)
where keratinocytes are made.
Keratinocytes multiply very rapidly and travel from the basal layer to the surface
The skin cannot shed these cells quickly enough, so they build up, leading to
thick, dry patches, or plaques.

Methodology
17 Adult patients with
moderate to severe
plaque-type
psoriasis
RNA extraction
phenol/chloroform
extraction

glass-fiber filter
purification

RT-PCR

RT-PCR amplifications

VEGF-A and PlGF

skin punch biopsy in

plaque lesion (PSO),
pinpoint lesions
(PP) and in the
distant uninvolved
skin (N)

embedded in OCT and

kept at 80 8C.
total volume of tissue used for
extracting RNA = number of sections
multiplied by their surface
and thickness.

amplification products were

separated by polyacrylamide gel
electrophoresis, stained
and quantified

discrimination of
the various splice variants
on the basis of the size of
their
amplification product

Fixed in 3% formalin
And embedded in
paraffin
immunohistochemistr
y
Ki-67
von Willebrand factor
anti-VEGFR3

blood and lymphatic

vessels expansion
measured by
computer-assisted
morphometry

Results
Blood vasculature expanded in PP to
an extent similar to PSO.
lymphatic vascular network in PP
suggests that lymphatic vessel
development is posterior to the blood
vessels expansion in psoriasis.

The expression of
VEGF-A 121, 165
and 189 was
significantly
increased in PP
and PSO as
to N.to N,
compared
As compared

VEGFR2, NRP-1,
Acanthosis and papillomatosis scores were
PlGF-1 and PIGF-2
evaluated for the three types of lesions (N,
and VEGF-C and
PP and PSO)
NRP-2a were
Increased in PP as compared to N
increased in PP and
Papillomatosis and acanthosis scores in Immunostaining of cycling cells (Ki-67) and lymphatic vesselsin
(D2-40)
in early psoriasis skin
PSO
lesion (PP) and psoriasis plaque (PSO). (a) and (b) Illustrate representative KiPP lie between N and PSO indicating
67 staining of PP and PSO, respectively showing positive blood endothelial cells in dermal
papillae (arrows). Serial sections in an early psoriasis lesion (c, d, g) and in a psoriasis
that these PP are evolving lesions
plaque (e, f, h)were also stainedwithD2-40 (c and e) and Ki-67 (d, f, g, h) in order to identify
endothelial proliferating lymphatic cells (arrowheads in g and h)
towards plaques
The average surface of individual blood
VEGFR3 was
vessels, identified by their positivity for von
increased in PSO
Willebrand factor in PP was significantly
when compared to N
higher than in N but was however
VEGFR-3 and marker
significantly smaller than in PSO
prox-1 showed
The percentage of superficial dermis
intermediate values in
area
PP
immunostaining of blood and lymphatic
occupied by lymphatic vessels was
vessels in uninvolved psoriatic skin (N),
early psoriasis skin lesion (PP) and psoriasis
significantly higher in PP than in N and
plaque (PSO) stained with anti von

Conclusion

Pinpoint lesion. This

photograph shows psoriasis
lesions including a pinpoint
lesion (arrow).

Psoriasis
plaques

Expansion of lymphatic vessels occurs after blood vascular

development in psoriasis.
Expansion of BV in PP could be followed by vessel
enlargement during progression to PSO, in parallel with a
decreased VEGF-A 189/VEGF-A 121 balance in plaques.