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BVB
BVB
Methods:
Macro method using wintrobe method which
is no longer used in routine use b/c it takes
big amount of blood & more time to PPT.
Normal range:
Men:
5 + 0.5 x 106 cells /L
5 + 0.5 x 1012 cells /L
method:
20 L from EDTA blood
+
4mL from diluent(formal citrate)=4000L
20L of blood
1
4000L of diluent
200
N
?
0.02L
1L
= 5X106 Cell/L
=5X1012 Cell/L
Used for:
Diagnosis and classification of anaemia.
MCV = PCV% x 10
RBC
0.45L/L
5X1012 /L
Simplified calculation:
MCV=PCV % X10=45X10= 90 FL
RBC W/O 10
5
12
Unit:Picogram (Pg)
MCH=Hb g/dlx10
RBC
=150
=30=30X10-12 g=30 Pg
5X1012
1012
Simplified calculation:
MCH=Hb g/dl
X10=15x10=30 pg
RBC W/O 10
5
12