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8-Metabolism
8-Metabolism
An Introduction into
Metabolism
Remember that cells (and organisms)
must obey the physical laws of the
universe
The problem
Cells and organisms are ordered
and must maintain that order.
Ie: This can not
happen
indefinitely
Free-energy change
(G) of reactions tells
us whether a metabolic
reaction occurs
spontaneously
final state
-G
initial state
-G
G = G
final state
-G
initial state
+G
Uphill Reaction
Coupled Reactions
Exergonic
Energy
Released
&
Harveste
d!
Endergonic
Energy
Required
Coupled reactions
Exergonic and Endergonic reactions are
coupled
NRG
er
go
ni
cR
ea
ct
io
ns
ADP +
ATP
Pi
End
e
rgo
n
ic R
eac
tion
s
Overall (couple
d)
Transport Work
Chemical Work
Review: Metabolism
The sum of all chemical reactions in the
body.
A chemical reaction
dissected
Reactants/Products
Reactant
s
Products
A chemical reaction
dissected
Look at free NRG (G)
of
Reactants
Products
Transition state
Transition state is a
higher energy state
Activation energy
EA
Enzyme
Products
Coenzymes
Organic molecule:
most vitamins
External Inhibitors
From outside the body
Like a toxin/poison
Allosteric regulators
Purposeful regulation
Allosteric site
Reversible or Irreversible
Attachment
Allosteric
regulation
Active form
Oscillation
- Noncompetitive
regulation
- Usually involves multiple
subunit enzymes
Inactive form
- Reversible
Activation
Allosteric activator
Inhibition
Allosteric inhibitor
Cooperativity
Substrate
(not really allosteric
control)
Regulation by
Localization
Localization of enzymes used in
common pathway
- Increases speed
- Greater control
Self-quiz
In this branched metabolic pathway, a red arrow
with a minus sign symbolizes inhibition of a
metabolic step by an end product (feedback
inhibition):