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Bprocess Principles-Module
Bprocess Principles-Module
Bprocess Principles-Module
PRINCIPLES
Stages in an Industrial
Bioprocess
Strain selection
Laboratory process development
Pilot Scale up
Industrial Scale up
Fermentation Media Preperation
Downstream process development
Product packaging techniques
Other Commercial consideration
Waste Disposal
March 26, 2015
Stages in an Industrial
Bioprocess
Stages in an Industrial
Bioprocess
An Ideal STRAIN
Genetic Stability
Efficient production of target product
Limited need for vitamins and growth
factors
Utilization of wide range of low cost
substrates
Safety, GRAS
Ready harvesting
Limited by products production
Easy genetic amenability
March 26, 2015
Stages in an Industrial
Bioprocess
Strain Selection
How?
Purchase
Stages in an Industrial
Bioprocess
Stages in an Industrial
Bioprocess
Stages in an Industrial
Bioprocess
Screening
What is screening?
Primary Screening
Secondary Screening
ENRICHMENT : Anearobes in N2
Atmospheres, Acidophiles in Extreme pH
values (2-4)
TEST SYTEMS: For Amylases the test
system would be agar plates with starch,
selection of colonies after staining with
iodine
Stages in an Industrial
Bioprocess
Stages in an Industrial
Bioprocess
Genetic
Engineering
Incorporation
into
artificial plasmids of
genes from a wide
variety of sources has
made possible the
transfer of genetic
material
across
virtually any species
barrier.
Mutation
Via chemical
or physical,
and biological
means
Stages in an Industrial
Bioprocess
Stages in an Industrial
Bioprocess
Scale Up
L)
Stages in an Industrial
Bioprocess
Stages in an Industrial
Bioprocess
F&I10
Traditional Bioprocess
DSP of Yeast Biomass
collect cells
wash cells
dewater cells
achieve desired consistency
package
store until shipped
Stages in an Industrial
Bioprocess