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What is the infective stage of the

Plasmodium spp?

a.
b.
c.
d.

Merozoite
Hypnozoite
Sporozoite
Gametocyte

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Page 1

HRP (Histidine rich protein) is


associated with what protozoan
parasite?
Plasmodium falciparum

COPT or cirumoval precipitin test is


the definitive method of choice for the
detection of what parasite?
Schistosoma or S. japonicum
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Page 2

Jack and Jill were admitted in the hospital


because both were experiencing fever and
severe headache. History reveals both
spent their summer in the hotsprings of
Mambucal. Unfortunately, Jack died 72
hours after admission. Brain biopsy
revealed focal hemorrhages and tissue
softening
consistent
with
amebae
infiltration. What is the most probable
causative agent that are consistent with
the findings?
a.
b.
c.
d.

Acanthamoeba
Cyclospora
Isospora belli
NaegleriaTemplates
fowleri
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The ONPG test allows organisms to be


classified as lactose fermenters by the
presence of what?
a.
b.
c.
d.

Permease
B-galactosidase
Beta lactamase
Phosphatase

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What is the stain in Aman medium?

a.
b.
c.
d.

Cotton blue
Methylene blue
Prussian blue
Bromthymol blue

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Page 6

Which of the following media identifies


species of Aspergillus?

a.
b.
c.
d.

Urease medium
Rice agar
Czapeks agar
Ascospore

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A presumptive identification of
Candida albicans can be made by
checking the ability of the organism to
produce what structure in serum?
a.
b.
c.
d.

Blastospores
Chlamydospores
Germ tube
Pseudohyphae

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Page 8

A yeast form with a single broad-based


bud would identify an organism as?

a.
b.
c.
d.

Blastomyces
Coccidioides
Histoplasma
Sporothrix

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Blastomyces dermatitidis Broad


based yeast
Paracoccidioides brasiliensis
Mariners wheel
Histoplasma capsulatom small
budding yeast with MACROPHAGES
Coccidioides immitis SPHERULE
with endospore

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Page 10

The Taxo P disc is used for the


identification of?

a.
b.
c.
d.

Haemophilus influenzae
Group A streptococci
Streptococcus pneumoniae
Streptococcus agalactiae

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Children who have infections with Bhemolytic streptococci can develop?

a.
b.
c.
d.

Chronic glomerulonephritis
Acute glomreulonephritis
Acute pyelonephritis
Nephrosis

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Page 12

What do you call the cytoplasmic


granules produced by C. diptheriae?

a.
b.
c.
d.

Babes Ernst granules


Much granules
Glycogen granules
NOTA

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Bacteria that grow favorably with cold


enrichment?

L. Monocytogenes
Y. eneterocolitica

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Colonies!
a. Mercury droplet colonies Bordetelle
pertussis
b. Bulls eye colonies in CIN Y. enterocolitica
c. Dew drop colonies/Satellitism H. influenzae
d. Medusa head colonies B. anthracis
e. Molar tooth colonies Actinomyces
f.

Fried egg Mycoplasma pneumoniae


(Atypical pneumonia)
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Page 15

Mueller Hinton media for Susceptibility


testing (MF)
Depth 4 mm
pH 7.2 to 7.4

Enterobacteriaciae!
All ferment glucose
All reduce nitrates to nitrites
All except SKY are motile.
H2s (+): SPACEd) Salmonella,
Proteus, Arizona, Citrobacter,
Edwardsiella)
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What microorganism can be identified


as having bipolar bodies or be
described as having a safety pin
appearance?

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All cocci are gram (+) except:


Neisseria
Branhamella
Veilonella
All bacilli are gram (-) except:
Bacillus
Clostridium
Mycobacterium
Listeria
Corynebacterium
Erysopelothrix
Lactobacillus
Fungus like: Nocardia and
Actinomyces
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Viridans streptococci Subacute


Bacterial endocarditis
Neisseria spp are identified through
carbohydrage fermentation on CTA
(Cystine Trypticase Agar)
N. Gonorrheae
N. Mengitidis
N. lactamica

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Bacteria motility!
Tumbling/ Umbrella Listeria
Darting - Campylobacter
Gliding - Capnocytophaga

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H. Influenzae Pfeifers bacillus


H. Aegypticus Koch-Weeks bacillus
(pink eye)
H. Ducreyi smallest pathogenic
bacteria; school of fish arrangement
H. Pylori peptic ulcer

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A five-year old child was brought to the hospital


upon the persistence of swelling (edema),
particularly around they eyes and in the extremities
and unexplained weight gain. Upon routine
urinalysis, it was revealed that the specimen had a
4+ protein, 4+ RBC parameters. Microscopic
examination revealed oval fat bodies, moderate
hematuria and some renal tubular cells. These are
most consistent with?

a.
b.
c.
d.

Acute glomerulonephritis
Goodpasture syndrome
Acute pyelonephritis
Nephrotic syndrome

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Page 23

The Clinitest is used to confirm the presence of


what chemical constituent in the urine?

a. Glucose and lactose only


b. Glucose, lactose and fructose only
c. Glucose, lactose, fructose and maltose
only
d. Glucose, lactose, fructose, maltose,
galactose only
e. Galactose, lactose, fructose, maltose,
galactose and sucrose.

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Page 24

What microscopic artifact found in synovial fluid


which is described as macroscopically resembles
polished rice and microscopically shows collagen
and fibrin?

a.
b.
c.
d.

Cartilage cells
Rice bodies
LE cells
RA cells

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Page 25

CSF protein levels is approximately how many


percent of the serum protein?

a.
b.
c.
d.

Less than 1%
Less than 10%
Less than 30%
Less than 40%

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Page 26

P-dimethyaminobenzaldehyde is the reagent used


in?

a.
b.
c.
d.

Hoesch test
Watson-Schwartz
Ehrilich Tube Test
Blondheims test

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Page 27

Curschmann's spirals refers to a finding in


the sputum of spiral shaped mucus plugs. They are
usually associated with what condition?

a.
b.
c.
d.

Bronchitis
Asthma
Pneumonia
Pharyngitis

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Page 28

If in doubt, which of the following microscopic urine


constituents can differentiate acute cystitis from
acute pyelonephritis?

a.
b.
c.
d.

RBC casts
Hyaline casts
WBC casts
Renal Tubular Epithelial cell casts

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Page 29

What is the 11th parameter in the urine strip?

a.
b.
c.
d.

Tammhorsfall protein
Ascorbic acid
Renal Tubular epithelial cells
Trichomonas vaginalis

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Page 30

One afternoon, a semen sample is submitted to the


laboratory. The specimen looks grossly normal,
however the amount submitted measured only 1
mL. Moreover, the patient claims that he has
abstained for three days. What should the medical
technologist do?

a. Proceed with the usual routine


procedures, but dilute the specimen when
performing sperm count to achieve even
distribution.
b. Process the specimen as any normal
semen is processed
c. Call the attention of the patient and ask for
a repeat collection of the sample and tell
him not to lie about the required
abstinence.
d. Call the patients physician and inform him
of the samples volume and proceed to
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examination
upon approval of physician.
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Which IgG subclass could not cross the placenta?

a.
b.
c.
d.

IgG1
IgG2
IgG3
IgG4

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First to appear

a. IgM

Best in agglutination

b. IgM

Best in precipitation

c. IgG

Ab found as dimer in secretions

d. IgA2

Least abundant Ab

e. IgE

2nd Ab to appear on surface of


lymph

f.

Measure TOTAL IgE

g. RIST

Measure IgE specific allergen

h. RAST

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IgD

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Serological Identity
(Ouchterlony DD)

a. Smooth curve

Nonidentity

b. Crossed lines

Partial identity
c. Spur formation
Aggln rxn where ANTIGEN is
attached to a carrier particle d. Passive Agglutination
Aggln rxn where ANTIBODY
is attached to a carrier
e. Reverse Passive
particle
Agglutination
Aggln rxn where bacteria is
used as carrier particle
f.

Coagglutination

(+) result for


g. No Agg. (+) viral Ab
hemagglutination inhibition
(B-HCG, Ab to influenza and
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rubella)
Page 35

Staining pattern of IFA


Homogenous

a. Anti DNP,
Rheumatoid

Peripheral

b. ACTIVE stage of
SLE; anti ds DNA

Speckled/ Mottled

c. Anti Smith for SLE,


anti RNP for MCTD,
RA, SLE

Nucleolar

d. Abs to nucleolar
RNA; (+)
Scleroderma

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Page 36

HBV Ag indicator of active


infection; first to appear

a. HBsAg

HBV Ag that indicates high


degree of infectivity

b. HBeAg

HBV Ag that is not detectable in c.


serum

HBcAg

HBV Ab indicator of current or


recent infection

d. Anti HBc IgM

HBV Ab marker of
convalescence

e. Anti Hbe IgG

Marker of immunity

f.

Anti HBsAg

HIV confirmatory
Western blot
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Page 37

ABO phenotype with the greatest


amount of H Antigen; strongest
reaction to anti H

a. O
b. A1B

With the least amout of H


antigen;
ABO phenotype that has anti
A,B, H

c.

Do distinguish True Group O


from an H null, what lectin is
used?

a. Ulex europaeus

Auto anti P is found in patients


with what condition?

b. PCH

Pathologic anti I is found in


patients with what condition?

c.

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Bombay

CAD

Page 38

McLeod phenotype cells are


associated with what antigen?

a. Kell antigen
(CGD)

Marker for African Black Race

b. Duffy

Served as a tool for tracing


Mongolian ancestry

c. Diego

CuSO4; drop method


Blood : Anticoagulant ratio in
blood bag

a. 1 Acg: 7 blood
(63 mL:450 mL)

CPDA1, how many days?


C Ag
P inc ATP
D energy
A improved RBC survival
SAGM and CPDA2 42 days

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Page 40

What is the allowable CV for TAG?

a.
b.
c.
d.

3%
4%
5%
6%

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Page 41

Which of the following checks the balance between


pituitary and ovary respectively?

FSH and estrogen


Hyperthyroidism dec cholesterol
Hypothroidism inc cholesterol
Creatinine clearance as a test is?
a.Reliable
b.Very reliable
c.Not reliable
d.Slighly reliable
Lpa sinking pre B lipoprotein
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Page 42

Ability of an analytical method toa. Sensitivity


measure smallest concentration
of analyte
To measure only the analyte of b. Specificity
interest
To give repeated results on the c. Precision
same sample
Degree by which it is easily
repeated

Practicability

Ability of the test to detect the Diagnostic sensitivity


proportion of the population with
the disease and who results
positively with the test
Without the disease and results Diagnostic specificity
are negative with the test
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Page 43

Error of precision ; indeterminate


error

a. Random error

Error of accuracy

b. Systematic error

What is the expected shape of a


Gaussian curve given that the
values are evenly distributed?

c.

Westgard Control Rules

d. Odd Random
(12s,13s, R4s)
Even Systematic
(22s, 41s,10x,
trend,shift)

Bell shaped curve

Is the commonly used patient


e. Delta check
based QC technique. Confirming
patient results with previous history
of the same test.
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Page 44

Gold standard for drug testing;


Confirmatory

GC-MS

Ion Selective electrodes for potassium,


oxygen, CO2

Valinomycin
Clark
Severinghaus

The only hormone that decreases


glucose levels

Insulin`

Reference method for glucose


Determination

Hexokinase

Used to monitor glucose levels for the


past 2-3 weeks

Fructosamine (glycated
albumin)

What lipoprotein is found in obstructive Lipoprotein X


jaundice and LCAT deficiency?
Also known as floating
B lipoprotein
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dysbetalipoproteinemi

State Beers Law.. :D


Determines concentration by measuring
light emitted by an electron that has
been raised a higher energy state and
has returned to its original state after
exposure to flame
Involves measurement of light
transmitted by a solution to measure
solute concentration
Measures electromagnetic radiation
emitted by atoms and not merely
molecules.
Determines amount of light blocked py
a particulate matter in a solution

AAS
Spectrophotometry
FEP
Nephelometry
Turbidimetry

Determines amount of scattered light by


particualte matter in a solution
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Page 46

Reference method for lipoprotein


quantitation

Ultracentrifugation

Fearon Rxn is used in the measurement


of?

Urea (DAM)

What do you call the method used to


measure creatinine in the presence of
alkaline picrate?

Jaffe reaction

Most commonly used method for


protein determination; reference
method

Biuret method; Kjeldahl


method

Electrophoresis pattern!
Gamma spike
B-G bridging
A2-globulin spike
Spikes in A1, A2 and B globulin
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Multiple myeloma
Hepatic cirrhosis
Nephrotic syndrome
inflammation
Page 47

It expresses acid-base relationship and


relates the pH of a solution to the
dissociation properties of the weak
acid.

Henderson Hasselbalch
Equation

For each degree of FEVER in patient,


pO2 will fall by 7% and pCO2 will rise
3%

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Page 49

Recall na lang!
Aperture plugs Most common cause of error in
automation; positive error
Angle of slides when making smears 30 -40
Other name of blood smear wedge smear, push smear
Hct tube length, bore, spin time and speed
WBC and RBC count
Extrinsic and Intrinsic factors
Most abundant coagulation factor in tissue
Excess heparin in anti thrombolytic therapy = Bleeding
Plt clumping = Inc RBC, dec plt
RBC clumping = dec RBC, inc WBC
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Westergren

Wintrobe

Capillary tube

Length

30 cm (300 mm)

11.5 cm (115 mm)

7 cm

Bore

2.5 mm

3.0

1 mm

LEFT
ESR
RED
RigHt
White
Hct

0
100
100
0

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