Professional Documents
Culture Documents
Culture Media
Culture Media
Culture Media
HOSPITAL KARACHI
Definition, purpose/importance
History of culture media
Growth pattern of bacteria
Classification of culture media
Microbiological culture
Method of cultivating microbial
organisms by letting them
reproduce in predetermined
culture media under controlled
laboratory conditions.
Bacteria
Continued.
Agar
Used for preparing solid medium
Obtained from seaweeds.
No nutritive value
Not affected by the growth of the
bacteria.
Melts at 98oC & sets at 42oC
2% agar is employed in solid medium
1. Lag phase
This is period of intense physiologic adjustment
involving the induction of new enzymes and the
synthesis and assembly of ribosome. In lag phase
and during this phase there occur
1. increase in size of cells
2. increase in metabolic rate
3. adaptation to new environment and
necessary enzymes.
Continued.
3. Stationary phase
In stationary phase after some time a stage
comes when rate of multiplication and death
becomes almost equal it may be due to
a. depletion of nutrient
b. accumulation of toxic products and
sporulation may occur during this stage.
II.
Special media
Enriched media
Enrichment media
Selective media
Indicator media
Differential media
Transport media
III. Based
on Oxygen requirement
- Aerobic media
- Anaerobic media
Complex media
Media other than basal media.
They have added ingredients.
Provide special nutrients
Synthetic or defined media
Media prepared from pure chemical
substances and its exact composition
is known
Eg: peptone water 1% peptone +
0.5% NaCl in water
Enriched media
Blood agar
Chocolate agar
Enrichment media
Liquid media used to isolate
pathogens from a mixed
culture.
Media is incorporated with
inhibitory substances to
suppress the unwanted
organism.
Eg:
Selective media
The inhibitory substance is added to a
solid media.
Eg:
Mac Conkeys medium for gram
negative bacteria
TCBS for V.cholerae
LJ medium M.tuberculosis
Wilson and Blair medium S.typhi
Potassium tellurite medium
Diphtheria bacilli
TCBS
LJ media
Indicator media
These media contain an indicator
which changes its colour when a
bacterium grows in them.
Eg:
Blood agar
Mac Conkeys medium
Christensens urease medium
Urease medium
Differential media
A media which has substances
incorporated in it enabling it to
distinguish between bacteria.
Eg: Mac Conkeys medium
Distinguish between lactose
fermenters & non lactose fermenters.
Transport media
Media used for transporting
the samples.
Delicate organisms may not
survive the time taken for
transporting the specimen
without a transport media.
Eg:
Anaerobic media