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Biokimia Karbohidrat, Metabolisme Energi, Dan Ketegenesis
Biokimia Karbohidrat, Metabolisme Energi, Dan Ketegenesis
Departme
An Overview of Metabolism
Summary of Metabolism
DIET
Carbohydrates
Fats
Proteins
Lipogenesis
Fat
stores
Lipogenesis
Excess glucose
Glucose
Glycogenesis
Glycogen
stores
Lipolysis
Urine
Protein
synthesis
Body
protein
Glycogenolysis
Glucose pool
Free fatty
acid pool
Excess nutrients
Amino
acids
Metabolism in
most tissues
Gluconeogenesis
Range of normal
plasma glucose
Amino acid
pool
Brain
metabolism
Figure 22-2
Summary of Metabolism
DIET
Carbohydrates
Fat
stores
Lipogenesis
Excess glucose
Glucose
Glycogenesis
Glycogen
stores
Urine
Glycogenolysis
Glucose pool
Range of normal
plasma glucose
Metabolism in
most tissues
Copyright 2007 Pearson Education, Inc., publishing as Benjamin Cummings
Brain
metabolism
Figure 22-2 (1 of 4)
Metabolism
Summary of biochemical pathways for energy production
Glycogen
Glucose
Glucose 6phosphate
Liver only
G
L
Y
C
O
L
Y
S
I
S
Glycerol
Some
amino
acids
NH3
Cytoplasm
2 ATP
Anaerobic conditions
Pyruvate
Lactate
Aerobic conditions
Pyruvate
CoA
Electron transport
system
O2
Acetyl CoA
Citric
acid
cycle
NH3
ATP + H2O
Mitochondria
Fatty
acids
Ketone
CO2
bodies
(in liver)
2 ATP
Some
amino
acids
Figure 22-3
Metabolism
Push-pull control of metabolism
Figure 22-4
Metabolism
Carbohydrate Metabolism
Primarily glucose
Fates of Glucose
Fed state
Storage as glycogen
Storage as lipids
Liver
Skeletal muscle
Adipose tissue
Fasted state
Synthesis
Synthesis and
and
breakdown
breakdown occur
occur at
at
all
all times
times
regardless
regardless of
of state...
state...
The
The relative
relative rates
rates of
of
synthesis
synthesis and
and
breakdown
breakdown change
change
Muscle
Insulin:
Glucagon
Glucose absorbed
Glycogen
Glucose absorbed
Adipose
Cells
Glucose absorbed
Glucose Metabolism
Glucose Utilization
Energy
Stores
Pentose
Phosphate
Pathway
Glycolysis
Glucose Utilization
Energy
Stores
Pentose
Phosphate
Pathway
Glycolysis
Glycogenesis
Liver
Glycogenesis
Skeletal muscle
1% of wet weight
Glucose Utilization
Energy
Stores
Pentose
Phosphate
Pathway
Glycolysis
Glycolysis
Lactate (animals)
Glucose Pyruvate Acetyl-CoA (TCA cycle)
Ethanol (yeast)
Glycolysis
Glucose + 2 ADP + 2 Pi
2 Lactate + 2 ATP + 2 H2O
Glycolysis - Summary
Glucose
2 ATP
4 ADP
2 ADP
4 ATP
2 NAD
2 NADH + H
2 Pyruvate
carbohydrate metabolism
Pyruvate Metabolism
Anaerobic Metabolism
of Pyruvate
Problem:
Anaerobic Metabolism
of Pyruvate
Solution:
NAD+
COO
C
CH3
Pyruvate
(oxidized)
COO
Lactatedehydrogenase
HC
OH
CH3
Lactate
(reduced)
Anaerobic Metabolism
of Pyruvate
ATP yield
Pyruvate
Lactate
NADH
NAD+
Cori Cycle
Pyruvate Metabolism
Pyruvate metabolism
Ketoglutarate
COO
C
CH3
Pyruvate
Keto acid
COO
Alanineaminotransferase
(AAT)
HC
NH3+
CH3
Alanine
Amino acid
Pyruvate Metabolism
Glycolysis
Pyruvate Dehydrogenase
Complex (PDH)
Aerobic Conditions
Electron
Transport
Chain
TCA Cycle
PDH - Summary
Pyruvate
2 NAD
2 NADH + H
CO2
Acetyl CoA
TCA Cycle
Dalam kondisi aerobik Link siklus TCA piruvat
untuk fosforilasi oksidatif
Terjadi pada mitokondria
Menghasilkan 90% dari energi yang
dilepaskan dari makanan
Metabolizes carbohydrate, protein, and fat
Strateginya adalah untuk mengoksidasi
asetil-KoA menjadi CO2 dan menangkap
energi NADH (FADH2) dan ATP
1 ATP
3 NAD
1 ADP
3 NADH + H
1 FAD
1 FADH2
Oxidative Phosphorylation
Oxidative Phosphorylation
and the Electron Transport
System
NADH + H+
FADH2
Glucose Utilization
Adipose
Energy
Stores
Glycogen
Glucose
Pentose
Phosphate
Pathway
Ribose-5-phosphate
Glycolysis
Pyruvate
Produces NADPH
Similar to NADH
Required for fatty acid synthesis
Ribose
Used for synthesis of nucleic acids
6-Phosphogluconolactone
6-Phosphogluconate
D-Ribulose5-phosphate
RNA or DNA
D-Ribose5-phosphate
Glucose Utilization
Adipose
Energy
Stores
Glycogen
Glucose
Pentose
Phosphate
Pathway
Ribose-5-phosphate
Glycolysis
Pyruvate
Energy Storage
Fates of Glucose
Fed state
Storage as glycogen
Storage as lipids
Liver
Skeletal muscle
Adipose tissue
Fasted state
Synthesis
Synthesis and
and
breakdown
breakdown occur
occur at
at
all
all times
times
regardless
regardless of
of state...
state...
The
The relative
relative rates
rates of
of
synthesis
synthesis and
and
breakdown
breakdown change
change
Gluconeogenesis
Necessary process
Fasting Situation
Glycogenolysis
Kerusakan atau mobilisasi glikogen yang disimpan oleh glukagon
Glukagon - hormon yang disekresi oleh pankreas selama masa puasa
Muscle
Insulin:
Glucagon
Glycogen
Glucose released
Adipose
Cells
Glycerol, fatty acids released
Fasted-State Metabolism
FASTED-STATE METABOLISM
Liver glycogen becomes glucose.
Liver
glycogen
stores
Glycogenolysis
Energy
production
Energy production
Glucose
Glycogen
Gluconeogenesis
Pyruvate
or
Lactate
Glucose
Energy production
Figure 22-7 (2 of 5)
Gluconeogenesis
Glycerol
Amino acids
Lactate
Supply carbon skeleton
Pyruvate
Propionate
There is no glucose synthesis from fatty acids
Fasted-State Metabolism
Adipose lipids
become free
fatty acids and
glycerol that
enter blood.
FASTED-STATE METABOLISM
Liver glycogen becomes glucose.
Liver
glycogen
stores
Glycogenolysis
Energy
production
Glucose
Free fatty
acids
-oxidation
Triglyceride stores
Free fatty
Glycerol
acids
Gluconeogenesis
Ketone
bodies
Energy production
Glycogen
Gluconeogenesis
Proteins
Pyruvate
or
Lactate
Glucose
Ketone
bodies
Energy production
Brain can
use only glucose
and ketones for energy.
Amino
acids
Figure 22-7
Fasted-State Metabolism
FASTED-STATE METABOLISM
Liver glycogen becomes glucose.
Liver
glycogen
stores
Glycogenolysis
Energy
production
Glucose
Glucose
Energy production
Figure 22-7 (1 of 5)
Fasted-State Metabolism
FASTED-STATE METABOLISM
Liver glycogen becomes glucose.
Liver
glycogen
stores
Glycogenolysis
Energy
production
Energy production
Glucose
Glycogen
Gluconeogenesis
Proteins
Pyruvate
or
Lactate
Glucose
Amino
acids
Energy production
Figure 22-7 (3 of 5)
Fasted-State Metabolism
Adipose lipids
become free
fatty acids and
glycerol that
enter blood.
FASTED-STATE METABOLISM
Liver glycogen becomes glucose.
Liver
glycogen
stores
Free fatty
acids
Glycogenolysis
Energy
production
Triglyceride stores
Free fatty
Glycerol
acids
Gluconeogenesis
Energy production
Glucose
Glycogen
Gluconeogenesis
Proteins
Pyruvate
or
Lactate
Glucose
Energy production
Amino
acids
Figure 22-7 (4 of 5)
Fasted-State Metabolism
Adipose lipids
become free
fatty acids and
glycerol that
enter blood.
FASTED-STATE METABOLISM
Liver glycogen becomes glucose.
Liver
glycogen
stores
Glycogenolysis
Energy
production
Glucose
Free fatty
acids
-oxidation
Triglyceride stores
Free fatty
Glycerol
acids
Gluconeogenesis
Ketone
bodies
Energy production
Glycogen
Gluconeogenesis
Proteins
Pyruvate
or
Lactate
Glucose
Ketone
bodies
Energy production
Brain can
use only glucose
and ketones for energy.
Amino
acids
Figure 22-7 (5 of 5)
K ETO G EN ESIS
Reactions of Ketogenesis
Ketone bodies
74
K ETO G EN ESIS
Badan keton diproduksi terutama di
mitokondria hepatosit.
Sintesis terjadi sebagai respons terhadap
kadar glukosa rendah dalam darah, dan
setelah kehabisan simpanan karbohidrat
seluler, seperti glikogen.
Produksi badan keton kemudian
berinisiatif untuk membuat energi
tersedia yang disimpan sebagai asam
lemak.
K ETO G EN ESIS
Namun, jika jumlah asetil-KoA yang
K ETO G EN ESIS
REVIEW!
Asam lemak menjalani
-oxidation to form
acetyl-CoA.
Biasanya, asetil-CoA
selanjutnya teroksidasi
dan energinya ditransfer
sebagai elektron untuk
NADH, FADH2, dan GTP
dalam siklus Krebs.
K ETO G EN ESIS
K ETO G EN ESIS
The three ketone bodies are:
Acetoacetate - if not oxidized to form usable
acetyl-CoA molecules.
K ETO G EN ESIS
Ketogenesis mungkin atau tidak mungkin
K ETO G EN ESIS
Ketika tubuh tidak memiliki karbohidrat bebas
K ETO G EN ESIS
K ETO G EN ESIS
K ETO G EN ESIS
Badan keton dibuat pada tingkat moderat
K ETO G EN ESIS
Konsentrasi tinggi abnormal badan keton
Ketosis
Ketosis occurs:
In diabetes, diets high in
fat, and starvation.
As ketone bodies
accumulate.
When acidic ketone
bodies lowers blood pH
below 7.4 (acidosis).
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