Professional Documents
Culture Documents
Thanh (Tom Tat Luan Van)
Thanh (Tom Tat Luan Van)
NGUYN TH THANH
Ngi hng dn 1
PGS.TS.NGUYN TH L ANH
Ngi hng dn 2
TS.NGUYN THANH HI
H NI - 2015
NI DUNG
PHN 1. M U
PHN 4:
PHN I: M U
1. t vn
Vit Nam nn y hc c truyn rt pht trin, nhiu cy dc liu qu
c s dng trong c cy a hong. Cy c ngun gc t Trung
Quc c khong cch y 2000 nm trc n nm 1958 nhp trng
nc ta, hin nay c pht trin trng nhiu ni t Bc n
Nam.R a hong thu hi v, ra nhanh, phn loi c to, nh ring,
em sy t t n khi c mm ri phi hay sy nhanh n kh. Loi c
to dng ngay (dng sng), tn thuc l sinh a. Dc liu sau khi c
ch bin c tn thuc l thc a.
Theo cc nghin cu gn y, a hong c rt nhiu tc dng tt c y
hc hin i quan tm. Nghin cu cng b, dch chit, c chit
t c a hong ti theo phng php hin i, dch chit ny c tc
dng cm mu, c ch t bo ung th, chng lot d dy, bo v gan,
tng cng min dch cho c th, iu chnh ni tit v chuyn ha.
2. Mc ch v yu cu
Mc ch
Xc nh c yu t k thut lm c s cho vic xy dng quy
trnh nhn nhanh v to c in vitro cy a hong phc v cho
nhu cu sn sut cy a hong nc ta
Yu cu
Xc nh c ch kh trng mu to ngun nguyn liu cho
nui cy
Xc nh c mi trng nui cy nhn nhanh in vitro
Xc nh c mi trng to r cho cc chi in vitro vn m
Xc nh mi trng to c in vitro
Xc nh c gi th v ch dinh dng cho cy in vitro vn
m
nh gi c sinh trng, pht trin v nng sut ca cy ging in
vitro trong iu kin t nhin
y.
3 KT QU V THO LUN
3.1.nh hng ca HgCl2 0,1% kt hp Sodium dichloroisocyanuric axit
(NaDCC 0.5%) n hiu qu kh trng.
Bng 3.1. : nh hng ca HgCl2 0,1% kt hp Sodiumdichloroisocyanuric axit
(NaDCC 0.5%) n hiu qu kh trng.
Cng
Thi gian
T l
T l mu
thc
(pht)
mu
sch(%)
T l mu sch
HgCl2
NaDC
nhim
T l mu
T l mu
0,1%
C 0.5%
(%)
sng(%)
cht (%)
CT1
70,2
29,8
80,5
19,5
CT2
10
34,3
65,7
63,2
36,8
CT3
15
21,7
78,3
31,6
68,4
BA
(mg/l)
T l
Chiu cao S l trung
S chi ti
mu to sinh(chi/mu)
TB
bnh(l/mu
chi(%)
chi(cm)
)
CT1
(C)
10
1,0
3,21
7,43
++
CT2
0,5
100
3,85
3,35
8,32
+ ++
CT3
1,5
100
3,50
4,16
5,85
+ ++
CT4
100
6,87
5,65
7,97
+ ++
CT5
100
2,42
3,47
4,17
+ ++
Cht
lng
chi
CT1:C MS
CT3:MS + 1mg BA
BA
NAA
T l mu
H s nhn
Chiu
S l TB
Cht
(mg/l)
(mg/l)
to
(chi/mu)
cao chi
(l/mu)
lng
chi(%)
CT1(C)
TB (cm)
chi
10
0,5
5,76
7,5
++
0,1
100
4,78
2,57
5,27
+ ++
0,3
100
6,53
3,38
6,9
+ ++
0,5
90
3,07
1,94
5,73
+++
0
CT2
CT3
CT4
CT3
CT2
CT1:C
Cng thc
CT1(C)
BA
(mg/l)
CT2
CT3
CT4
CV(%)
LSD0.05
Nc
da
(mg/l)
T l
mu to
chi (%)
H s nhn
(chi/mu)
Chiu
cao
chi
TB
(cm)
S l TB
(l/mu)
7,43c
1,95b
10,77a
50
8,00b
1,96b
11,17a
100
8,23b
1,97a
b
11,43a
150
8,77a
2,02a
11,57a
2,31
1,4
4,4
0,35
0,05
0,93
Cht
ln
g
chi
Cng thc
H s nhn
(chi/mu)
Chiu cao
chi TB (cm)
S l
TB(l/mu)
7.43b
1,95b
10,77b
0,5
6,9c
1,91b
10,77b
1,0
6,9c
1,94b
10,77b
1,5
8,43a
1,84c
11,4ab
2,0
8,77a
2,02a
11,57a
CV(%)
3,1
1,26
3,8
LSD0.05
0,44
0,04
0.76
CT1(
C)
BA
(mg/l)
CT2
CT3
CT4
CT5
Pepton
g/l)
T l mu
to chi (%)
Cht
lng
chi
CT1(C)
BA
Manito
(mg/l)
(g/l)
T l mu
to
chi(%)
H s nhn
(chi/mu)
Chiu cao
chi TB
(cm)
T l
S l
cy b
TB(l/mu)
thy
tinh
CT2
CT3
10
CT4
15
Cht
lng
chi
T l
T
Cng thc
BA
Thiosunfat Bac
(mg/l)
(M/l)
H s
mu
nhn
to
(chi/mu)
chi(%)
Chiu
cy
cao
S`l TB
khng
chi TB
(l/mu)
b thy
(cm)
tinh(%
)
CT1(C)
85,5
2,73a
3,30bc
7,50a
4,5
CT2
10
100
2,23b
3,50b
6,70b
22,4
20
93,2
2,80a
3,93a
6,67b
31,2
30
100
2,20b
3,13bc
7,03ab
26,3
CV(%)
4,78
4,93
4,34
LSD0.05
0,22
0,32
0,57
CT3
CT4
Cng thc
BA
T l mu
H s nhn
Chiu
S l
T l cy b
Cht
(mg/l)
to chi
(chi/mu)
cao chi
TB(l/mu)
Thy tinh
lng
(%)
CT1:n
TB (cm)
chi
thng
CT2: n
tm
Cng thc
MS0+30g/l
saccaroza
MS0+30g/l
saccaroza
MS0+30g/l
saccaroza
MS0+30g/l
saccaroza
CV(%)
LSD0.05
-NAA
(mg/l)
T l ra r
(%)
Chiu
cao TB
(cm)
S l TB
(l/cy)
S`r
TB(r/cy)
Chiu
di`r TB
(cm)
94,5
3,57c
6,40ab
5,00c
2,23d
0,1
100
3,77c
6,37ab
5,33c
2,57c
0,3
100
5,47a
6,57a
6,30a
3,57a
0,5
100
4,27b
6,13b
5,77b
3,13b
4,74
0,38
2,60
0,31
3,30
0,35
5,59
0,30
CT1
CT3
Cng thc
IBA (mg/l)
CT1 (C)
CT2
CT3
CT4
CV(%)
LSD0.05
0
0,1
0,3
0,5
Chiu
T l ra
cao TB
r (%)
(cm)
90,20
95,74
100
100
3,53b
3,80b
4,77a
3,70b
4,13
0,31
S l
trung bnh
(l/cy)
S r TB
(r/cy)
Chiu di
r TB (cm)
6,50b
6,47b
7,27a
5,87c
2,76
0,34
2,30c
3,67b
4,53a
4,77a
4,72
0,34
3,47d
4,63c
5,90a
5,43b
3,25
0,30
Cng thc
MS0 + 30g /l
saccaroza + 0mg/l
-NAA
MS0 + 30g /l
saccaroza +0,5
mg/l -NAA
MS0 + 30g /l
saccaroza 1mg/l NAA
MS0 + 30g /l
saccaroza 1,5
mg/l -NAA
MS0 + 30g /l
saccaroza 2 mg/l
-NAA
CV(%)
T l ra r Chiu cao
(%)
TB (cm)
S l
trung
bnh
(l/cy)
S c TB
(c/cy)
Chiu
di c
TB (cm)
Khi lng
TB c(g)
Khi lng
ti
100
3,10c
5,33b
3,20d
2,13d
0,87c
100
3,40b
5,57b
3,70c
2,60c
1,23b
100
4,53a
5,60b
4,27b
3,63a
1,33b
100
4,43a
5,93a
4,60a
3,20b
1,54a
100
3,53b
5,47b
3,53c
2,80c
1,22b
3,11
2,81
3,60
4,02
4,66
Cng thc
Chiu
cao TB
(cm)
S l
trung
bnh
(l/cy)
S c TB
(c/cy)
Chiu di
c TB
(cm)
Khi lng TB c
(g)
Khi
lng
ti
Khi
lng
kh
5,50b
2,50b
1,12c
0,19b
5,90a
2,77a
1,54a
0,26a
5,63ab
2,70ab
1,34b
0,20b
CV(%)
LSD0.05
2,75
0,31
3,97
0,21
1,66
0,04
5,37
0,02
3,82
0,23
1,87
0,27
Cng thc
S c
Chiu
TB(c/cy di c
)
TB (cm)
Chiu cao
TB (cm)
S l trung
bnh(l/cy)
3,33d
5,37b
3,10d
3,60c
5,70a
4,83a
Khi lng TB
c(g)
Khi
lng
ti
Khi
lng
kh
2,30d
0,93d
0,10d
3,57c
2,70c
1,33c
0,17c
5,67a
4,17b
3,77a
1,60a
0,24a
4,53b
5,87a
4,70a
3,37b
1,47b
0,22b
2,45
1,98
2,78
3,56
5,30
5,10
1,19
0,21
0,20
0,20
0.13
0,18
T l sng
Chiu cao TB
S l trung
(ngy)
(%)
(cm)
bnh(l/cy)
t ph sa
86,67
2,75b
7,37b
93,33
2,98a
8,13a
t ph sa + mn da ( 1:1)
92,00
2,94a
8,20a
96,67
3,01a
8,37a
CV(%)
2,82
4,37
LSD0.05
0,16
0,66
Cng thc
T l sng
Chiu cao
S l trung
trng (ngy)
(%)
TB (cm)
bnh(l/cy)
C( phun nc l)
93,33
13,77c
6,03d
Yogen 30-10-10
96,67
13,50b
7,17b
Atonik
96,67
17,47a
7,73a
u tru 502
93,33
14,40c
6,73c
CV(%)
2,35
2,77
LSD0.05
0,72
0,38
Cng thc
4. KT LUN V NGH
4.1. Kt lun
i vi mu chi a hong, cht kh trng thch hp l HgCl 2 0,1% thi gian kh trng l 5
pht kt hp NaDDC 10 pht vi t l mu sch sng l 63,2 %.
- Mi trng nui cy khi ng thch hp to chi t mu cy ban u mi trng MS
b sung 1mg/l BA tt nht cho kh nng nhn nhanh chi invitro cy a hong.
- Vi mi trng MS b sung 1mg/l BA kt hp vi 15% nc da cho h s nhn chi
cao nht t 5,43 chi/mu.
- Cht khng Ethylene ( Thiosunfat Bc ) vi hm lng 20 M/l khi cho MS + 1BA c h
s nhn chi cao nht l 93,2%, chi khng b thy tinh l 31,5%
- Gi th gim cy thch hp nht cho cy a hong invitro l gi th tru hun + mn da
1:1. Trn gi th ny t l sng t 96,67%, chiu cao cy 3,01 cm, s l 8,37 l/cy.
- S dng phn bn l atonik thch hp cho s sinh trng cy a hong invitro ngoi
vn m. Kt qu so vi i chng th khi phun phun bn l Atonik chiu cao t
17,47cm.
4.2. ngh:
Tip tc nghin cu hon thin quy trnh to c invitro cy a hong, nghin cu cc bin
php chng hin tng thy tinh ha cy a hong invitro.