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Collagen Presentation: Biochemistry
Collagen Presentation: Biochemistry
C O L L A G E N P R E S E N TAT I O N
GROUP MEMBERS:
NATASHA ALLADIN
DAVENDRA CARTER
JONELLE EUROPE
LATIFA HENRY
THERESA
JAIKISHUN
TOSNAUSHA
LOGAN
AZALIA LUKE
SHARICE RAZACK
TAMARA WILLIAMS
OBJECTIVES
1. Outline the structure and biochemical function of collagen
Types
Structure
Function and Biosynthesis
INTRODUCTION
body
It functions to provide mechanical support and structure
most abundant
TYPES OF COLLAGEN
Type
Tissue distribution
Fibril forming
ii
iii
iv
Basement membrane
vii
ix
xii
Cartilage
Tendon ligaments
1. Fibril-forming collagens:
Types I, II, and III are the fibrillar collagens; Have
TYPE II
Cartilaginous
structures
TYPE III
Distensible tissues
e.g. Blood vessels
2. Network-forming collagens:
Types IV and VII form a three-dimensional mesh,
STRUCTURE OF COLLAGEN
Amino acid sequence
Each polypeptide chain has precisely 1050 amino acid
residues.
The primary structure of collagen is rich in glycine and
proline.
Proline facilitates the helical conformation of each alpha
The -chains are then twisted around one another in a rope-like manner to produce the
overall tightly packed triple-helical form of the molecule.
The main bonds associated are inter hydrogen bonds and covalent cross links.
About 1/3 of the AA composition of collagen is gly. It is the smallest AA which allows
three strands to sit closer to each other to form a tight coil.
Collagen has a repetitive sequence of gly-x-y . For each gly-x-y triplet a hydrogen bond
if formed between the amide H atom of gly and the carbonyl O atom of residue X in at
adjacent chain.
Proline allows the formation of a tightly wound helical conformation, because its ring
structure causes kinks in the polypeptide chain.
HYDROXYPROLINE AND
HY DRO XY LYCI NE
Hydroxyproline is essential in stabilizing the
BIOSYNTHESIS
Preprocollagen
Hydroxylation
Glycosylation
Procollagen
Triple Helix
Cleavage
Tropocollagen
Collagen
Cross Links
Functions of collagen
Strength
Bone
Blood
Vessels
Flexibility / Elasticity
Support/ Structure
Bone is made up of a mixture of collagen and a mineral called hydroxyapatite. The two work
together to form the structure, flexibility and strength of your bones.
Collagen makes up the walls of the veins, arteries and capillaries of the body. This gives the
vessels strength, structure and flexibility, all of which are needed to effectively transport blood
throughout your entire body
Collagen fibers provide your muscles with the strength and structure needed to move and
Muscles
function throughout the day. Collagen fibers makes up your skeletal muscle fibers, smooth
muscles) and cardiac muscles.
Skin
Collagen makes up
approximately 80 percent of
the dry weight of your skin. It
provides structure to the skin
D E G R A D ATI O N
Causes of collagen to break down
Collagenase -enzyme
Too much sugar in the diet -increase glycation
Sunlight - damages the fibers and induces irregular elastin
S AVE TH E C O L L A G EN
reconstructive surgeries
Collagen as bone substitutes e.g. cosmetic
surgeries
Collagen as bioengineered tissues
COLLAGEN DISEASES/
DISORDERS
1. Collagen biosynthesis defects
. The best-known is Scurvy, results from a dietary deficiency of
C O L L A G E N VAS C U L A R D I S E A S E S
Rheumatoid arthritis (RA) - the immune system attacks the thin membrane
(called the synovium) lining the joints, causing pain, stiffness, warmth and
swelling of the joints, and inflammation throughout the body.
Polymyositis and dermatomyositis - two related diseases in which there is
GENETIC DISORDERS OF
COLLAGEN BIOSYNTHESIS
collagen.
Potentially lethal vascular problems occur.
Patients also show defects in collagen type I fibril, which result in
type I collagen.
The structurally abnormal chains prevent folding of the protein into
triple-helical conformation.
REFERENCES
Jones D, Hosalkar H, Jones S. The orthopaedic management of
osteogenesis imperfecta. Clin Orthop. 2002. 16:374-88.
Zeitlin L, Fassier F, Glorieux FH. Modern approach to children with
osteogenesis imperfecta. J Pediatr Orthop B. 2003 Mar. 12(2):77-87.
[Medline].
Forin V. [Paediatric osteogenesis imperfecta: medical and physical
treatment]. Arch Pediatr. 2008 Jun. 15(5):792-3. [Medline].
Esposito P, Plotkin H. Surgical treatment of osteogenesis imperfecta:
current concepts. Curr Opin Pediatr. 2008 Feb. 20(1):52-7. [Medline ].
http://onlinelibrary.wiley.com/doi/10.1002/bip.360210507/abstract
http://www.biochemj.org/bj/316/0001/3160001.pdf