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High Performance Liquid Chromatography
High Performance Liquid Chromatography
Liquid Chromatography
Chem. 331
Introduction
HPLC system
Partition chromatography
Adsorption, or liquid-solid
chromatography
COMPOSITION OF A LIQUID
CHROMATOGRAPH SYSTEM
Solvent
Solvent Delivery System (Pump)
Injector
Sample
Column
Detectors (Diode Array)
Waste Collector
Recorder (Data Collection)
Uses of HPLC
HPLC columns
Instrument Parameters
Column Material
Deactivation
Stationary Phase
Coating Material
Temperature
Flow
Signal
Sample Sensitivity
Detector
Concentration
Matrix
Solvent Effect
Sample Effect
phase
Reverse
Size
Ion
phase
exclusion
exchange
Normal phase
In
Reverse phase
In
Size exclusion
In
Ion exchange
In
Selectivity Factor
K values
GLC
GSC
SFC (Supercritical
Fluid)
Types of Detectors
Absorbance (UV
with Filters, UV with
Monochromators)
Evaporative Light
Scattering Detector
(ELSD)
Electrochemical
IR Absorbance
Fluorescence
MassSpectrometric
Refractive-Index
Photo-Diode Array
EVALUATION PARAMETERS
EFFICIENCY
RESOLUTION
INERTNESS
RETENTION INDEX
COLUMN BLEED
CAPACITY FACTOR
References
http://192.215.107.101/ebn/942/tech/techfocus/1071main.html
http://www.chem.usu.edu/~sbialk/Classes/565/opamps/opamps.ht
ml
Skoog, Holler, and Neiman. Principles of Instrumental Analysis.
5th ed. Orlando: Harcourt Brace & Co., 1998.
http://weather.nmsu.edu
http://elchem.kaist.ac.kr/vt/chem-ed/sep/lc/hplc.htm
http://www.chemistry.nmsu.edu/Instrumentation/Lqd_Chroma.html
http://weather.nmsu.edu/Teaching_Material/SOIL698/Student_Mat
erial/HPLCHP1090/HPLCINJ.HTM
http://testequipment.globalspec.com/LearnMore/Labware_Scientific_Instru
ments/Analytical_Instruments/Chromatographs/HPLC_Columns
http://www.chemistry.adelaide.edu.au/external/soc-rel/content/lccol.htm