There are three sections to this course: @ AU5800 Hardware Component Overview @ISE Unit Overview @ Software OverviewAt the end of this course you will be able to: @ Identify the major hardware components of the AUS5800 and know what they do. ® Identify the components of the ISE unit and understand how it operates. ® Identify the elements of the Home Screen of the AU5800 Software and understand how to navigate through it. —s Follow this link to download er | AU5800 product documentation: {a https://techdocs.beckmancoulter.comAU5800 Model Numbers Model Number: AU58XY AU58 = 5800 Series X = Number of Analyzer Units Y = Number of ISE Flowcells 1 analyzer unit +2 ISE flowcells = AU5812Rack Feeder Unit ISE Unit Analyzer UnitLicht tla I eS ‘Touchscreen Monitor Keyboard Wash Solution Mouse Tank1, Rack Set Unit Rack set trays are placed here. This is where routine samples are loaded (on the analyzer. 2 & 3. Set Trays Set trays are used to load many sample racks at once on the analyzer. Each rack holds ten samples, and each rack set tray holds twenty racks. This gives a capacity of 400 samples when both sot trays are filed. 4. Rack Buffer Unit This area temporarily holds up to 23 sample racks before transferring them fo the Rack Transfer Unit, Racks may be held here before either the intial analysis or repeat analysis. 5. Priority Rack Set Unit This area allows the loading of one or two Priority racks. Racks loaded here will be procassed ahead of racks loaded in the Rack Set Area. 6. Vertical Rack Transfer Unit This device moves racks between th Rack Set Unit (1), Rack Butfer (4), and Rack Collection Unit (8). 7. Rack Transfer Unit ‘This unit has three lanes that are used fo move racks fo the chemistry analysis modules. Lane "a" is the primary lane and handles routine racks; Lane "b"is the bypass lane, ‘and Lane "e"is the return lane. 8. Rack Collection Unit This area holds two rack collection trays. Sample racks are retumed here when testing is complete. 9 & 10. Collection Trays Each collection tray holds up to 20 sample racks. Racks that are ready {or removal will collect here.Rack Set UnitsRack Collection UnitsPriority LoadingClick a number to see a description of that component1. Sample Probes S1 and S2 In conjunetion with the sample syringes, these two probes aspirate sample from the sample container and dispense the sample into a cuvette. 1 dalivers sample to the inner cuvette wheel and S2 delivers sample to the outer cuvette wheel 2. Cuvette Wheels The inner and outer wheels hold a total of 408 glass cuvettes. Reactions occur and absorbance readings are taken here. 3&4. Reagent Refrigerators These reftigerated compartments hold the R1 (#3) and R2 (#4) reagents. There are 54 positions in each refrigerator. 5. R1 Reagent Probes In conjunction with a reagent syringe, these probes aspirate R1 reagents from the R1 reagent refrigerator and dispense them into cuvettes. R1-1 delivers to the inner cuvette wheel, and R1-2 delivers to the outer cuvette wheel 6. R2 Reagent Probes In conjunction with a reagent syringe, these probes aspirate R2 reagents from the R2 reagent refrigerator and dispense them into cuvettes. R2-1 delivers to the inner cuvette wheel, and R2-2 delivers to the outer cuvette, wheel 7. R1/S Mix U Mix bars on this unit mix the contents of the cuvelte after the addition of reagent R1 and again after the addition of sample. The mix bars on this unit are spiral shaped.8. R2 Mix Unit Mix bars on this unit mix the contents of the cuvette after the addition of reagent R2. The mix bars on this unit are L-shaped. 9. Wash Nozzle Unit This mechanism washes, rinses, and cries the cuvettes before and after sample analysis,1. R1 Syringes The R1 syringes work with the R1 reagent probes to aspirate reagent and deliver it to the cuvettes in the cuvette wheel. R1-1 delivers reagent to the inner wheel; R1-2 delivers reagent to the outer wheel. 2. R2 Reagent Syringes The R2 syringes work with the R2 reagent probes to aspirate reagent and deliver it to the cuvettes in the cuvette wheel. R2-1 delivers reagent to the inner wheel; R2-2 delivers reagent to the outer wheel. 3. Sample Syringes (not shown) The sample syringes are located on the opposite side of the Analyzer Unit. Working with the sample probes, these syringes aspirate sample from sample containers and deliver it to cuvettes in the cuvette wheel. 4. Analyzer Tank Storage The tanks showh here hold deionized water, wash solution concentrate, and cilute wash solution used throughout the system to wash cuvettes and mix bars.WASHING RINSING DRYING‘Tho Mixture Aspiration Rallor Pump pulls ISE Reference Solution from the bottle, past the Reference Electrode where measurements are taken, and then oul fo waste. ASPIRATION REFERENCE SOLUTION SOLUTIONFLOWCELL qi Hf || SAMPLE POT Cia) <2 i VALVES fabsvanoano wipstavoaro sesurrer SYRINGE ‘SOLUTION. SOLUTION‘The Mixture Aspiration Roller Pump pulls ISE Reference Solution from the botile, past the Reference Electrode where measurements are taken, and then out to waste. lk, ChISE Buffer Is delivered to the Sample Pot by the ISE Buffer Syringe. areal ASPIRATION REFERENCE,ISE Buffer is delivered ta the Sample Pot by tho ISE Buffer Syringe. oun fE_MIDSTANDARD MIDSTANDARD SE BUFFER ASPRATION REFERENCE SOLUTION. SOLUTION OLLER PUMP. ROLLER PUMP‘Sample is aspirated by tne Sample Probe and dispensed into the Sample Pot where the sample and ISE Buffer are mixed. ASPIRATION REFERENCE SOLUTION SOLUTION one pone ROLLER DUMPSample is aspirated by the Sample Probe and dispensed info the Sample Pot where the sample ‘and ISE Butter are mixed wverure SE __NIDSTANDARD MID STANDARD ISE BUFFER ROLLER PUN? ROLLER PUMPThe Mixture Aspiration Roller Pump draws the samplefbuffer mixture through the Flowoell where measurements are laken. Excess fluid in the Sample Potis pulled through the Bypass Tubing by the Mixture Aspiration Roller Pump and sent to waste. Ise BUFFER SYRINGE‘miter eau Unie Sarriprs, we 1SE BuNIsr Synge venUS IDE Buffer fo the Sample Pot. The ISE Buffer is pulled through the flowoell by the Mixture Aspiration Roller Pump to rinse. the flowoell. This stop is omitted if the sample type is, serum,‘Tho Mid Standard Solution Roller Pump pulls Mid Standard Solution from the boitle and delivers it to the Sample Pot, ‘The Mixture Aspiration Roller pump draws the Mid Standard Solution through the Flowcell where measurements are taken. Excess fluid in the Sample Pot is pulled through the bypass tubing by the Mixture Aspiration Roller Pump and sent to waste. ASPIRATION REFERENCE SOLUTION. SOLUTIONISE SampleSample Pots) Floweells Pinch Valves Mixture Aspiration Roller Pumps Mid Standard Solution Roller Pumps. Reference ElectrodesSample Pots (Flowcells) Pinch Valves Mixture Aspiration Roller Pumps Mid Standard Solution Roller Pumps Reference ElectrodesSample Pots Flowcells (Pinch Valves Mixture Aspiration Roller Pumps Mid Standard Solution Roller Pumps Reference ElectrodesSample Pots Flowcells Pinch Valves. Mixture Aspiration Roller Pumps Mid Standard Solution Roller Pumps Reference ElectrodesGRD cid. NEC bo Contra Sample Pots Flowcells Pinch Valves Mixture Aspiration Roller Pumps Mid Standard Solution} Roller Pumps. Reference ElectrodesSample Pots Flowcells Pinch Valves Mixture Aspiration Roller Pumps Mid Standard Solution Roller Pumps Reference Electrodes)True is Incorrect. The ISE Unit measures Na, K, and Cl, but not Ca or COz.MID Standard Solution What reagent is used to dilute the sample before analysis? (Click your choice) That is correct! Mid Standard is drawn through the flow cell between samples. Click to continue ©‘Touchscreen monitor Rack Feeder Unit‘Touchscreen monitor Keyboard Mouse Rack Feeder Unit Data Processing Unitaad ‘&@l STANDBY t Home Return to the Home ScreenSTANDBY co qj ———— t t Menu List Display the Menu.List screen"| OG t User Menu Display the User Menu List (configured by the user)BBE eos ME. —=9 O08 Ia t Mode Display Indicates the current system mode and may indicate time remaining@GSOSd la 4 Measure Start Start or restart analysis(OO © NOMEHZ t Pause Pause sampling (select Start to resume)@ = MOMENI 4 Feeder Stop Stop the Rack Feeder unit01/16/2013 15:21 4 Stop / Standby Stop analysis -allin progress data will be lost Return to Standby -from a Stop conditionZi 4 Onboard Help Access the AU5800 User's Guide and Maintenance Videos01/16/2013 21 4 Log Out Log out the current user01/16/2013 15:21 4 End Process Shut down the system and turn off the auxilliary power supply‘Current hardware component status Current temperature values\ de Jump Buttons Start Condition ene Cee ee PoE \ eeReagent Irae eee Serge ea) Management Maintenance Create a data index, which includes setting the index date and time, start sample number, group of tests and operator nameStart Condition i Rack Requisitio Check detailed R1 and R2 reagent status, calibration information, volume of reagents available and cleaning solution status for ISE and analyzer unitsAnalyzer Start Condition Mana Maintenance Access the analyzer and ISE maintenance menus and perform maintenance operationscr art Condition Peetearns Use to requisition calibrations, quality control or patient samplesStart Condition Peercaens Maintenance View, print and batch transfer to host computer reagent blank, calibration, QC and sample dataDieranpe ov i6a01 o> Me vicaars 10 | cme [I 5 SE Reece Slain Shor CSE sans fame or INDEX (POE TRATED Power Foire De Samp RTPeeRereE ovcccrs cero Pe ovnecors race ate Level | lari tmColoration > ROUTINE Basic operation menus for performing tests. includes setting start conditions, managing reagents, assigning racks, viewing sample status. programming samples/calibrations/QC, repeating results, and monitoring data.lease Q Sate > CALIBRATION Display and manage calibration results and histories.ac Display and edit Quality Control (ac) results and histories.@ i Caltrain ae # : i va sa a System > PARAMETERS alysis parameters (how the Systern'e configured), eludes common and specific. repeat parameters, calibration parameters, and QC parameters. parameters, LsMAINTENANCE Perform maintenance, view generated maintenance alarms, perform diagnostics.SYSTEM Customize the system setup. Includes: online communications, format reports, comments, system conditions, user menu creation, and external data management.oO © loli’: STAND Eernenae—" | & © © Teale:—“6 00s Iai]