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Micro - Antimicrobial Susceptibility Test
Micro - Antimicrobial Susceptibility Test
SUSCEPTIBILITY TEST:
Diffusion test procedure
GROUP 4
PURPOSE:
To show in-vitro sensitivity of
different bacteria to selected
antibiotics.
MATERIALS:
1.
2.
3.
4.
5.
6.
7.
a) It gives satisfactory
growth of the majority
of rapidly growing
pathogens
b) It is not antagonistic to
the major antimicrobial
agents to be tested
c) It is isotonic with blood,
can be used to perform
susceptibility tests on
certain fastidious
organisms that cannot
grow on
unsupplemented
medium
Organism:
1. Escherichia coli
2. Staphylococcus aureus
3. Salmonella typhi
4. Shigella dysnteriae
5. Pseudomonas aeroginosa
6. Proteus vulgaris
7. Klebsiella pneumoniae
8. Salmonella paratyphi A
9. Enterobacter cloacae
10. Salmonella enteritidis
Antibiotic used:
1.
2.
3.
4.
5.
Ampicillin
Chloramphenicol
Gentamicin
Penicillin G
Trimenthoprimsulfamethoxazole
(SXT)
PROCEDURE
1. Using an inoculating
loop, obtain an
inoculum from the
stock culture and
suspend directly into
a small volume of
sterile NSS. Repeat
this produce until the
turbidity matches
that of the BaSO4
comparison
standard.
Susceptibility
- This category indicates that the antimicrobial
agent in question maybe an appropriate choice for
the infection caused by the bacterial isolate tested
Intermediate
- The antimicrobial agent may still be effective
against the tested isolate but usually at higher
concentration
Resistant
- This category indicates that the microbial agent
in question may not be an appropriate choice for
treating the infection caused by the bacterial
isolate tested